MD02G1274100.v1.1

Belongs to the glycosyl hydrolase 31 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0018783
Physical Location & Seq
Reverse (-)
32864834 .. 32871575
6742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1274100.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGATCTCCGGAATGTGGGCAACGAGGCTTACGCGCACTCTGTTCTGCTGCTCAACAGCAATGGCATCGATGTGTTCTACAGAGGGACTTCTCTCACCTACAAGGTCATTGGGGGTGTTTTCGACTTTTACTTCTTTGCCGGGCCGACACCGTTGGGCGTTGTCAACCAGTACACGGCGTTCATCGGCAGACCGGCCCCGATGCCCTACTGGTCTCTTGTGAGGGAGTGGTGGCTTACAGTGTGCAAAACGAGGAGGGAATCATCACCGAACTCTACATCTTGGGAAAATGTTCGGAGACGAACAAATGATCACCAAGAAGGTGTGATAAAGGATGGAGGGAATGAAGTATTGTTTAATGACCAAATCATGAAGCGGAGGACAACATGGAGTGGTTCTGATTCTCATACCCTGCCACATGCTCAAACACATCAGACTCAGAGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_31

Protein Analysis

150

Amino Acids

17.01

Weight (kDa)

8.82

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018783)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21220 FvH4_1g21220 FvH4_1g28641 FvH4_1g28641 FvH4_2g24510
malus_domestica MD01G1098000.v1.1 MD02G1274100.v1.1 MD17G1274400.v1.1
pyrus_communis pycom17g27260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 35
AccIII TCCGGA 1 cut(s) 9
AciI CCGC 1 cut(s) 376
AclWI GGATC 1 cut(s) 12
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 2 cut(s) 16, 175
Alw26I GTCTC 2 cut(s) 219, 292
AlwI GGATC 1 cut(s) 12
Aor13HI TCCGGA 1 cut(s) 9
AoxI GGCC 2 cut(s) 143, 195
ApeKI GCWGC 1 cut(s) 49
Asp700I GAANNNNTTC 1 cut(s) 291
AspLEI GCGC 1 cut(s) 37
AspS9I GGNCC 2 cut(s) 143, 196
AsuC2I CCSGG 1 cut(s) 142
AsuHPI GGTGA 3 cut(s) 88, 258, 305
BbvI GCAGC 1 cut(s) 36
BccI CCATC 1 cut(s) 329
BceAI ACGGC 1 cut(s) 192
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BclI TGATCA 1 cut(s) 310
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 219, 292
BfmI CTRYAG 1 cut(s) 79
BisI GCNGC 1 cut(s) 50
BlsI GCNGC 1 cut(s) 51
Bme1390I CCNGG 1 cut(s) 142
BmgT120I GGNCC 2 cut(s) 143, 196
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 75, 192
BpuMI CCSGG 1 cut(s) 142
Bsa29I ATCGAT 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 219
BsaWI WCCGGW 1 cut(s) 9
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 175
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 2 cut(s) 169, 215
Bse3DI GCAATG 1 cut(s) 67
BseAI TCCGGA 1 cut(s) 9
BseCI ATCGAT 1 cut(s) 69
BseGI GGATG 1 cut(s) 340
BseLI CCNNNNNNNGG 2 cut(s) 16, 175
BseMI GCAATG 1 cut(s) 67
BseNI ACTGG 2 cut(s) 169, 215
BseRI GAGGAG 1 cut(s) 268
BseXI GCAGC 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 35
BshFI GGCC 2 cut(s) 145, 197
BshVI ATCGAT 1 cut(s) 69
BsiSI CCGG 3 cut(s) 10, 141, 194
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 2 cut(s) 16, 175
BsmAI GTCTC 2 cut(s) 219, 292
BsmBI CGTCTC 1 cut(s) 292
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 2 cut(s) 145, 197
Bso31I GGTCTC 1 cut(s) 219
Bsp13I TCCGGA 1 cut(s) 9
Bsp143I GATC 2 cut(s) 4, 310
BspACI CCGC 1 cut(s) 376
BspANI GGCC 2 cut(s) 145, 197
BspDI ATCGAT 1 cut(s) 69
BspEI TCCGGA 1 cut(s) 9
BspFNI CGCG 1 cut(s) 35
BspHI TCATGA 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 198
BspPI GGATC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 219
BsrDI GCAATG 1 cut(s) 67
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 2 cut(s) 169, 215
BssAI RCCGGY 1 cut(s) 193
BssMI GATC 2 cut(s) 4, 310
Bst4CI ACNGT 2 cut(s) 153, 241
BstDEI CTNAG 1 cut(s) 438
BstF5I GGATG 1 cut(s) 340
BstFNI CGCG 1 cut(s) 35
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 2 cut(s) 7, 313
BstMAI GTCTC 2 cut(s) 219, 292
BstMBI GATC 2 cut(s) 4, 310
BstNSI RCATGY 1 cut(s) 422
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 79
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu15I ATCGAT 1 cut(s) 69
BsuRI GGCC 2 cut(s) 145, 197
BsuTUI ATCGAT 1 cut(s) 69
BtsCI GGATG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 246
CciI TCATGA 1 cut(s) 369
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 143, 196
ClaI ATCGAT 1 cut(s) 69
Csp6I GTAC 1 cut(s) 172
CviAII CATG 3 cut(s) 370, 387, 419
CviJI RGCY 4 cut(s) 29, 145, 197, 235
CviKI_1 RGCY 4 cut(s) 29, 145, 197, 235
CviQI GTAC 1 cut(s) 172
DdeI CTNAG 1 cut(s) 438
DpnI GATC 2 cut(s) 6, 312
DpnII GATC 2 cut(s) 4, 310
Eco31I GGTCTC 1 cut(s) 219
Esp3I CGTCTC 1 cut(s) 292
FaeI CATG 3 cut(s) 373, 390, 422
FaiI YATR 4 cut(s) 371, 388, 408, 420
FaqI GGGAC 1 cut(s) 100
FatI CATG 3 cut(s) 369, 386, 418
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 50
FokI GGATG 1 cut(s) 347
Fsp4HI GCNGC 1 cut(s) 50
GlaI GCGC 1 cut(s) 36
GluI GCNGC 1 cut(s) 50
HaeIII GGCC 2 cut(s) 145, 197
HapII CCGG 3 cut(s) 10, 141, 194
HhaI GCGC 1 cut(s) 37
Hin1II CATG 3 cut(s) 373, 390, 422
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 3 cut(s) 260, 401, 436
HpaII CCGG 3 cut(s) 10, 141, 194
HphI GGTGA 3 cut(s) 88, 258, 305
Hpy166II GTNNAC 2 cut(s) 166, 174
Hpy188I TCNGA 4 cut(s) 297, 400, 435, 441
Hpy188III TCNNGA 2 cut(s) 10, 370
Hpy8I GTNNAC 2 cut(s) 166, 174
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 2 cut(s) 153, 241
HpyCH4V TGCA 1 cut(s) 246
HpyF3I CTNAG 1 cut(s) 438
Hsp92II CATG 3 cut(s) 373, 390, 422
HspAI GCGC 1 cut(s) 35
Kpn2I TCCGGA 1 cut(s) 9
Ksp22I TGATCA 1 cut(s) 310
Kzo9I GATC 2 cut(s) 4, 310
LpnPI CCDG 6 cut(s) 23, 154, 182, 196, 207, 425
Lsp1109I GCAGC 1 cut(s) 36
LweI GCATC 2 cut(s) 75, 192
MalI GATC 2 cut(s) 6, 312
MboI GATC 2 cut(s) 4, 310
MflI RGATCY 1 cut(s) 4
MluCI AATT 1 cut(s) 445
MlyI GAGTC 1 cut(s) 430
MnlI CCTC 7 cut(s) 19, 77, 216, 246, 249, 332, 372
MroI TCCGGA 1 cut(s) 9
MroXI GAANNNNTTC 1 cut(s) 291
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 71
MspI CCGG 3 cut(s) 10, 141, 194
MspR9I CCNGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 35
NciI CCSGG 1 cut(s) 142
NdeII GATC 2 cut(s) 4, 310
NlaIII CATG 3 cut(s) 373, 390, 422
NlaIV GGNNCC 1 cut(s) 198
NspI RCATGY 1 cut(s) 422
PagI TCATGA 1 cut(s) 369
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 2 cut(s) 260, 401
PkrI GCNGC 1 cut(s) 51
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 2 cut(s) 143, 196
PsrI GAACNNNNNNTAC 2 cut(s) 164, 196
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 1 cut(s) 50
Sau3AI GATC 2 cut(s) 4, 310
Sau96I GGNCC 2 cut(s) 143, 196
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 1 cut(s) 142
SetI ASST 3 cut(s) 101, 108, 325
SfaNI GCATC 2 cut(s) 75, 192
SfcI CTRYAG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 71
Sse9I AATT 1 cut(s) 445
SsiI CCGC 1 cut(s) 376
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 2 cut(s) 153, 241
TaqI TCGA 2 cut(s) 69, 123
TasI AATT 1 cut(s) 445
TatI WGTACW 1 cut(s) 171
TfiI GAWTC 2 cut(s) 260, 401
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 1 cut(s) 246
TseI GCWGC 1 cut(s) 49
TspDTI ATGAA 3 cut(s) 172, 360, 386
TspRI CASTG 1 cut(s) 246
XceI RCATGY 1 cut(s) 422
XmnI GAANNNNTTC 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.