Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0012714

Physical Location & Seq

Genomic Coordinates Forward (+)

37112131 .. 37115219

Gene Structure 3089 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1316600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3039 bp

ATGAAGCTGGTAGTAGAAGTGGTAGATGCTCATGATCTTATGCCCAAAGACGGCAAAGGATCTGCTATTCCATTTGTACAAGTCGATTTTGTGAACAAGCTTAGCCGAACCAAGACTATTCCGAAAAATCTCAACCCCATTTGGAACCAGAAACTTTTCTTTGATATTGACCAAACCAAATCCATCACCAAGCCATTGAAGTATCTGTTGAGAATTCCTTGCTTAAATATTGTCCAGAAAAGTGAGGAAGCTTATCAAAGATTCCAACTTGAAAACAAGTGGTTTTTCTCATCTGTCAAGGGTGAGACTGGCCTAAAAATCTATATTTCTCTAGAATCTGAACCGAAAGCTCCTCCATATTCTCCACCACAACCCATAGAAGCCTCTCCTTCCAAGTCTCAGCAACAAACATCCGAAAACTCAAGTTCTAGTCCCTTTGCTTCTCCAAATACAGAAAACACAAAATCCAATAGCAAAGTATTGGCTGCTATTCCAAAAGAAAAAGAGTTCAGACAAAAATCAGCTGAAGTAAGATCAACTGAAACATCCCACCACGTACACAAGCATCAAGTTCTGCAGCAACCAGGCATATCGGTAGCAACACAACCTCAAGGTTTCCAACTAATCATGCGGCCAGCTCACCTGGAAGCGCATCGTAGTCATAACCAGCAAGACGACTATGACTTGAAGGACACCAGCCCACAGCTTGGCGAAAGGTGGCCAAATGGTGGAGAGTACGGAGGAAGAGGATGGATGAGCGGTGGTGAAAGATTTACAAGTACTTATGACCTTGTTGAGCAGATGTTTTATCTGTATGTTCGAGTTGTGAAAGCCAAAGACCTCCCTCCCAGCTCCATTACCGGAAGCTGTGATCCTTACGTGGAAGTAAAGCTGGGGAATTACAAGGGAAGAACAAAGCATTTTGAGAGGAAGATGAATCCAGAGTGGAACCAGGTGTTTGCTTTCTCAAAAGACCGAATCCAGTCATCGGTGGTGGAAGTTTTTGTCAAAGATAAAGAGATGATTGGAAGAGATGATTATATTGGAAGGGTGGTTCTTGACTTGAATGAGGTTCCGAGCAGAGTTCCACCTGACAGCCCGCTCGCTTCTCAGTGGTACAGGCTGGAGCACCGCCGCGGGGAAGGGAAGGTTAGAGGTGAGATCATGCTTGCAGTCTGGATGGGAACACAGGCTGATGAAGCCTTTCCGGATGCATGGCATACGGATGCTGTAGCTGTCTATGGAGAGGGTGTGTTAAATATTCGATCCAAGGTATACGTATCACCAAAACTTTGGTACCTGAGGGTGAATGTGATTGAAGCTCAGGATGTGCTGCCTAACGACAGAAGCCGCCTCCCAGAAGTCTTTGTCAAAGCTCAGGTTGGGAACCAAGTGCTCAGAACCAAGCTATGCCCAACTCGAACTGCGAATCCATTGTGGAATGAAGATTTAGTCTTTGTTGCAGCTGAGCCTTTTGAGGAGCAGCTGGTTGTTACAGTTGAGGATCGAGTCCACCCTTTGAAAGACGAGGTGTTGGGTAAGATAAGCATGCCAATTGATGCGTTTGAGAAGCGGCTTGACCACAGGCCAGTTCACTCCCGCTGGTTCAATCTCGAGAAGTATGGTTTTGGTGTGTTGGAGCCTGATAGGAGGAATGAGCTAAAGTTTTCTAGTAGAATTCACCTGAGAGTTTGTCTTGAAGGTGGATACCATGTGCTAGATGAATCGACCATGTATATAAGTGATCAGAGGCCAACGGCAAGGCAGCTTTGGAAGCAGCCTGTTGGGATTTTGGAAGTTGGCATTCTAAGTGCACAAGGGCTTCTTCCAATGAAGATGAAGGACGGCCGAGGGAGCACAGACCCTTACTGTGTGGTTAGGTATGGCCAGAAATGGGTCCGTACCAGAACAATTATCGACACATTTAGTCCTAAGTGGAATGAGCAATACACATGGGAAGTGTACGATCCGTGCACCGTGATCACATTGGGAGTTTTTGACAACTGCCACCTAGGTGGAAGTGAGAAGCAACCACCAACTGCAGGCAGCGGAGCAAGAGATTCTCGAATTGGAAAGGTGCGTATTCGACTATCAACTCTTGAAGCTCATCGGATGTATACACATTCGTATCCTCTCCTTGTTCTACAGCCTAATGGAGTCAAGAAAATGGGCGAACTCCAACTAGCTGTTCGATTCACAACCCTCTCCATAGCTAACATGATATATGTTTATGGGCACCCCATGTTGCCAAAAATGCATTACTTGCATCCTTTCACAGTGAACCAAGTCGATAATCTACGATACCAAGCAATGAATATCGTAGCAGTAAGACTTGGCAGAGCTGAGCCGCCTCTGAGAAAAGAAGTGGTGGAGTACATGCTAGATGTTGATTCCCACATGTGGAGTATGAGGAGAAGCAAGGCCAACTTCTTCAGAATTATGTCATTGCTTTCTGGTATGTTTTCCATGAGCCGGTGGTTTGCTGATGTGTGCAACTGGAAGAACGGCGTCACCACTGTTCTTGTTCACATTCTCTTTCTGATACTGATCTGGTATCCAGAGTTGATACTTCCAACTCTTTTTGTCTACATGTTCCTCATCGGAATGTGGAATTACAGGTTCAGGCCTAGATATCCTCCTCATATGGATATCAAGCTTTCATGGGCAGAGACAGTTCACCCAGACGAGCTTGATGAAGAGTTTGACACATTTCCGAGCTCTAGACCCCACGATATAGTTCGAATGAGGTACGACAGGATCAGAAGTGTAGCAGGGAGGATACAGACAGTTGTCGGTGACATAGCAACGCAAGGGGAGAGATTTCAGTCGCTACTTAGCTGGAGAGACCCGAGAGCAACCAGCCTTTTCATATTGTTCTGCCTTTGTGCAGCTGTGGTGCTTTACGTGACTCCGTTCAGGGTGGTTTCTTTGGTTGCAGGGTTGTACAACTTGAGGCACCCCAAATTCCGGAGCAAGCTGCCTTCCGCACCCCACAATTTCTTCAAAAGACTGCCCGCTCAGACCGACAGCTTACTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005783 GO:0005829 GO:0012505 GO:0016020 GO:0016021 GO:0031224 GO:0031974 GO:0031981 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0070013

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 PRT_C

Protein Analysis

1013

Amino Acids

116.43

Weight (kDa)

9.19

Isoelectric Point (pI)

48.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	2 - 58	1.1e-11	C2 domain
C2	PF00168	270 - 375	5.5e-23	C2 domain
C2	PF00168	432 - 538	4.2e-19	C2 domain
C2	PF00168	596 - 712	2.7e-21	C2 domain
PRT_C	PF08372	857 - 1012	5e-80	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012714)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48060
fragaria_vesca	FvH4_7g00550
malus_domestica	MD02G1316600.v1.1 MD07G1005400.v1.1 MD14G1009800.v1.1
prunus_persica	Prupe.2G007600_v2.0.a1
pyrus_communis	pycom02g26530 pycom07g00440
rosa_chinensis	RchiOBHm_Chr1g0314341
rosa_laevigata	RLG00000030765
rosa_multiflora	Rmu_sc0000343.1_g000010 Rmu_sc0002833.1_g000008
rosa_roxburghii	Rroxscaffold_4G00331940
rosa_rugosa	Rorug01G0001200
rosa_samantha	Rh1AG010300 Rh1BG003200 Rh1DG005700
rosa_wichuraiana	Rw1G000480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1296
AccB1I	GGYRCC	3 cut(s)	1296, 2235, 2955
AccB7I	CCANNNNNTGG	2 cut(s)	707, 728
AccBSI	CCGCTC	3 cut(s)	759, 1102, 3017
AccI	GTMKAC	3 cut(s)	1275, 2117, 2586
AccII	CGCG	1 cut(s)	1137
AccIII	TCCGGA	2 cut(s)	1207, 2967
AclWI	GGATC	6 cut(s)	67, 866, 1260, 1512, 1961, 2765
AcoI	YGGCCR	4 cut(s)	632, 719, 1846, 1885
AcsI	RAATTY	3 cut(s)	213, 1677, 2963
AcuI	CTGAAG	2 cut(s)	546, 2416
AcyI	GRCGYC	1 cut(s)	2508
AflIII	ACRYGT	2 cut(s)	2397, 2589
AjnI	CCWGG	3 cut(s)	583, 642, 951
AjuI	GAANNNNNNNTTGG	2 cut(s)	2172, 2204
AleI	CACNNNNGTG	1 cut(s)	2013
AloI	GAACNNNNNNTCC	4 cut(s)	1038, 1070, 1414, 1446
Alw21I	GWGCWC	6 cut(s)	1131, 1398, 1816, 1859, 1976, 2720
Alw26I	GTCTC	4 cut(s)	299, 402, 2663, 2838
Alw44I	GTGCAC	2 cut(s)	1812, 1972
AlwI	GGATC	6 cut(s)	67, 866, 1260, 1512, 1961, 2765
Ama87I	CYCGRG	2 cut(s)	1613, 2848
Aor13HI	TCCGGA	2 cut(s)	1207, 2967
AoxI	GGCC	9 cut(s)	310, 632, 719, 1586, 1751, 1846, 1885, 2421, 2624
ApaLI	GTGCAC	2 cut(s)	1812, 1972
ApoI	RAATTY	3 cut(s)	213, 1677, 2963
Asp700I	GAANNNNTTC	2 cut(s)	155, 1203
Asp718I	GGTACC	1 cut(s)	1296
AspA2I	CCTAGG	1 cut(s)	2011
AspLEI	GCGC	1 cut(s)	652
AspS9I	GGNCC	1 cut(s)	1897
AsuII	TTCGAA	1 cut(s)	2740
AvaI	CYCGRG	2 cut(s)	1613, 2848
AvaII	GGWCC	1 cut(s)	1897
AvrII	CCTAGG	1 cut(s)	2011
AxyI	CCTNAGG	1 cut(s)	1301
BaeGI	GKGCMC	3 cut(s)	1816, 1976, 2238
BaeI	ACNNNNGTAYC	4 cut(s)	1280, 1313, 2111, 2144
BalI	TGGCCA	2 cut(s)	721, 1887
BanI	GGYRCC	3 cut(s)	1296, 2235, 2955
BanII	GRGCYC	1 cut(s)	2720
Bbv12I	GWGCWC	6 cut(s)	1131, 1398, 1816, 1859, 1976, 2720
BccI	CCATC	3 cut(s)	191, 744, 1174
BceAI	ACGGC	4 cut(s)	67, 1773, 1861, 2521
BciT130I	CCWGG	3 cut(s)	585, 644, 953
BciVI	GTATCC	4 cut(s)	1700, 2139, 2565, 2772
BclI	TGATCA	2 cut(s)	1744, 1980
BcoDI	GTCTC	4 cut(s)	299, 402, 2663, 2838
BfaI	CTAG	9 cut(s)	332, 429, 1671, 1718, 2012, 2183, 2381, 2628, 2721
BfmI	CTRYAG	4 cut(s)	575, 1230, 2040, 2144
BfuI	GTATCC	4 cut(s)	1700, 2139, 2565, 2772
BlnI	CCTAGG	1 cut(s)	2011
BlpI	GCTNAGC	3 cut(s)	101, 1467, 2343
BmcAI	AGTACT	1 cut(s)	781
Bme1390I	CCNGG	3 cut(s)	585, 644, 953
Bme18I	GGWCC	1 cut(s)	1897
BmeT110I	CYCGRG	2 cut(s)	1613, 2848
BmgT120I	GGNCC	1 cut(s)	1897
BmiI	GGNNCC	9 cut(s)	146, 950, 1074, 1298, 1388, 1641, 1898, 2237, 2957
BmrFI	CCNGG	3 cut(s)	585, 644, 953
BmsI	GCATC	7 cut(s)	16, 574, 661, 1201, 1216, 1549, 2275
BoxI	GACNNNNGTC	1 cut(s)	2789
BpmI	CTGGAG	2 cut(s)	1145, 2860
Bpu10I	CCTNAGC	2 cut(s)	1323, 1377
Bpu1102I	GCTNAGC	3 cut(s)	101, 1467, 2343
Bpu14I	TTCGAA	1 cut(s)	2740
BpuEI	CTTGAG	3 cut(s)	406, 594, 2971
BsaAI	YACGTR	4 cut(s)	556, 880, 1279, 2905
BsaBI	GATNNNNATC	1 cut(s)	2546
BsaHI	GRCGYC	1 cut(s)	2508
BsaI	GGTCTC	1 cut(s)	2838
BsaJI	CCNNGG	4 cut(s)	1135, 1269, 1849, 2011
BsaWI	WCCGGW	3 cut(s)	860, 1207, 2967
BsaXI	ACNNNNNCTCC	2 cut(s)	1236, 1266
Bse118I	RCCGGY	1 cut(s)	2472
Bse1I	ACTGG	4 cut(s)	313, 982, 1589, 2501
Bse21I	CCTNAGG	1 cut(s)	1301
Bse3DI	GCAATG	2 cut(s)	2316, 2444
Bse8I	GATNNNNATC	1 cut(s)	2546
BseAI	TCCGGA	2 cut(s)	1207, 2967
BseBI	CCWGG	3 cut(s)	585, 644, 953
BseDI	CCNNGG	4 cut(s)	1135, 1269, 1849, 2011
BseJI	GATNNNNATC	1 cut(s)	2546
BseMI	GCAATG	2 cut(s)	2316, 2444
BseNI	ACTGG	4 cut(s)	313, 982, 1589, 2501
BseRI	GAGGAG	4 cut(s)	342, 1493, 2425, 2628
BseSI	GKGCMC	3 cut(s)	1816, 1976, 2238
BseX3I	CGGCCG	1 cut(s)	1846
BseYI	CCCAGC	2 cut(s)	848, 892
BsgI	GTGCAG	1 cut(s)	2907
Bsh1236I	CGCG	1 cut(s)	1137
Bsh1285I	CGRYCG	1 cut(s)	1849
BshFI	GGCC	9 cut(s)	312, 634, 721, 1588, 1753, 1848, 1887, 2423, 2626
BshNI	GGYRCC	3 cut(s)	1296, 2235, 2955
BsiEI	CGRYCG	1 cut(s)	1849
BsiHKAI	GWGCWC	6 cut(s)	1131, 1398, 1816, 1859, 1976, 2720
BsiHKCI	CYCGRG	2 cut(s)	1613, 2848
BsiSI	CCGG	4 cut(s)	861, 1208, 2473, 2968
BslFI	GGGAC	1 cut(s)	417
BsmAI	GTCTC	4 cut(s)	299, 402, 2663, 2838
BsmFI	GGGAC	1 cut(s)	417
BsmI	GAATGC	1 cut(s)	1803
BsnI	GGCC	9 cut(s)	312, 634, 721, 1588, 1753, 1848, 1887, 2423, 2626
Bso31I	GGTCTC	1 cut(s)	2838
BsoBI	CYCGRG	2 cut(s)	1613, 2848
Bsp119I	TTCGAA	1 cut(s)	2740
Bsp1286I	GDGCHC	7 cut(s)	1131, 1398, 1816, 1859, 1976, 2238, 2720
Bsp13I	TCCGGA	2 cut(s)	1207, 2967
Bsp1407I	TGTACA	2 cut(s)	76, 2943
Bsp1720I	GCTNAGC	3 cut(s)	101, 1467, 2343
BspANI	GGCC	9 cut(s)	312, 634, 721, 1588, 1753, 1848, 1887, 2423, 2626
BspEI	TCCGGA	2 cut(s)	1207, 2967
BspFNI	CGCG	1 cut(s)	1137
BspHI	TCATGA	1 cut(s)	31
BspLI	GGNNCC	9 cut(s)	146, 950, 1074, 1298, 1388, 1641, 1898, 2237, 2957
BspMAI	CTGCAG	2 cut(s)	579, 2044
BspPI	GGATC	6 cut(s)	67, 866, 1260, 1512, 1961, 2765
BspT104I	TTCGAA	1 cut(s)	2740
BspT107I	GGYRCC	3 cut(s)	1296, 2235, 2955
BspTNI	GGTCTC	1 cut(s)	2838
BsrBI	CCGCTC	3 cut(s)	759, 1102, 3017
BsrDI	GCAATG	2 cut(s)	2316, 2444
BsrFI	RCCGGY	1 cut(s)	2472
BsrGI	TGTACA	2 cut(s)	76, 2943
BsrI	ACTGG	4 cut(s)	313, 982, 1589, 2501
BssAI	RCCGGY	1 cut(s)	2472
BssECI	CCNNGG	4 cut(s)	1135, 1269, 1849, 2011
BssNAI	GTATAC	2 cut(s)	1276, 2118
BssNI	GRCGYC	1 cut(s)	2508
BssT1I	CCWWGG	2 cut(s)	1269, 2011
Bst1107I	GTATAC	2 cut(s)	1276, 2118
Bst2UI	CCWGG	3 cut(s)	585, 644, 953
Bst4CI	ACNGT	7 cut(s)	1498, 1871, 1978, 2278, 2518, 2674, 2788
Bst6I	CTCTTC	3 cut(s)	739, 1024, 2691
BstACI	GRCGYC	1 cut(s)	2508
BstAPI	GCANNNNNTGC	1 cut(s)	2263
BstAUI	TGTACA	2 cut(s)	76, 2943
BstBAI	YACGTR	4 cut(s)	556, 880, 1279, 2905
BstBI	TTCGAA	1 cut(s)	2740
BstC8I	GCNNGC	8 cut(s)	636, 1100, 1104, 1170, 1550, 2044, 2975, 3015
BstDSI	CCRYGG	1 cut(s)	1135
BstFNI	CGCG	1 cut(s)	1137
BstHHI	GCGC	1 cut(s)	652
BstMAI	GTCTC	4 cut(s)	299, 402, 2663, 2838
BstMCI	CGRYCG	1 cut(s)	1849
BstMWI	GCNNNNNNNGC	5 cut(s)	1199, 1774, 1854, 2254, 2263
BstNI	CCWGG	3 cut(s)	585, 644, 953
BstNSI	RCATGY	4 cut(s)	1552, 2380, 2401, 2593
BstPAI	GACNNNNGTC	1 cut(s)	2789
BstSCI	CCNGG	3 cut(s)	583, 642, 951
BstSFI	CTRYAG	4 cut(s)	575, 1230, 2040, 2144
BstSLI	GKGCMC	3 cut(s)	1816, 1976, 2238
BstSNI	TACGTA	1 cut(s)	1279
BstUI	CGCG	1 cut(s)	1137
BstX2I	RGATCY	1 cut(s)	59
BstXI	CCANNNNNNTGG	2 cut(s)	1293, 2015
BstYI	RGATCY	1 cut(s)	59
BstZ17I	GTATAC	2 cut(s)	1276, 2118
BstZI	CGGCCG	1 cut(s)	1846
Bsu36I	CCTNAGG	1 cut(s)	1301
BsuI	GTATCC	4 cut(s)	1700, 2139, 2565, 2772
BsuRI	GGCC	9 cut(s)	312, 634, 721, 1588, 1753, 1848, 1887, 2423, 2626
BtgI	CCRYGG	1 cut(s)	1135
BtsIMutI	CAGTG	3 cut(s)	1118, 2283, 2514
Cac8I	GCNNGC	8 cut(s)	636, 1100, 1104, 1170, 1550, 2044, 2975, 3015
CciI	TCATGA	1 cut(s)	31
CfoI	GCGC	1 cut(s)	652
Cfr10I	RCCGGY	1 cut(s)	2472
Cfr13I	GGNCC	1 cut(s)	1897
Cfr42I	CCGCGG	1 cut(s)	1138
CseI	GACGC	1 cut(s)	2497
CsiI	ACCWGGT	1 cut(s)	951
CspCI	CAANNNNNGTGG	2 cut(s)	2503, 2538
EaeI	YGGCCR	4 cut(s)	632, 719, 1846, 1885
EagI	CGGCCG	1 cut(s)	1846
Eam1104I	CTCTTC	3 cut(s)	739, 1024, 2691
EarI	CTCTTC	3 cut(s)	739, 1024, 2691
Ecl136II	GAGCTC	1 cut(s)	2718
EclXI	CGGCCG	1 cut(s)	1846
Eco105I	TACGTA	1 cut(s)	1279
Eco130I	CCWWGG	2 cut(s)	1269, 2011
Eco147I	AGGCCT	1 cut(s)	2626
Eco24I	GRGCYC	1 cut(s)	2720
Eco31I	GGTCTC	1 cut(s)	2838
Eco32I	GATATC	2 cut(s)	2633, 2650
Eco47I	GGWCC	1 cut(s)	1897
Eco52I	CGGCCG	1 cut(s)	1846
Eco53kI	GAGCTC	1 cut(s)	2718
Eco57I	CTGAAG	2 cut(s)	546, 2416
Eco81I	CCTNAGG	1 cut(s)	1301
Eco88I	CYCGRG	2 cut(s)	1613, 2848
EcoICRI	GAGCTC	1 cut(s)	2718
EcoRI	GAATTC	2 cut(s)	213, 1677
EcoRII	CCWGG	3 cut(s)	583, 642, 951
EcoRV	GATATC	2 cut(s)	2633, 2650
EcoT14I	CCWWGG	2 cut(s)	1269, 2011
EcoT22I	ATGCAT	2 cut(s)	1216, 2259
EcoT38I	GRGCYC	1 cut(s)	2720
ErhI	CCWWGG	2 cut(s)	1269, 2011
FalI	AAGNNNNNCTT	4 cut(s)	1809, 1841, 2411, 2443
FaqI	GGGAC	1 cut(s)	417
FauI	CCCGC	4 cut(s)	1107, 1130, 1607, 3022
FauNDI	CATATG	1 cut(s)	2643
FbaI	TGATCA	2 cut(s)	1744, 1980
FblI	GTMKAC	3 cut(s)	1275, 2117, 2586
FriOI	GRGCYC	1 cut(s)	2720
FspBI	CTAG	9 cut(s)	332, 429, 1671, 1718, 2012, 2183, 2381, 2628, 2721
GlaI	GCGC	1 cut(s)	651
GsaI	CCCAGC	2 cut(s)	852, 896
GsuI	CTGGAG	2 cut(s)	1145, 2860
HaeIII	GGCC	9 cut(s)	312, 634, 721, 1588, 1753, 1848, 1887, 2423, 2626
HapII	CCGG	4 cut(s)	861, 1208, 2473, 2968
HgaI	GACGC	1 cut(s)	2497
HhaI	GCGC	1 cut(s)	652
Hin1I	GRCGYC	1 cut(s)	2508
Hin6I	GCGC	1 cut(s)	650
HinP1I	GCGC	1 cut(s)	650
HindIII	AAGCTT	3 cut(s)	98, 249, 2654
HpaII	CCGG	4 cut(s)	861, 1208, 2473, 2968
HpyAV	CCTTC	8 cut(s)	399, 682, 1041, 1136, 1141, 1694, 1834, 2991
HpyCH4III	ACNGT	7 cut(s)	1498, 1871, 1978, 2278, 2518, 2674, 2788
HpyCH4IV	ACGT	4 cut(s)	555, 879, 1278, 2904
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1199, 1774, 1854, 2254, 2263
HpySE526I	ACGT	4 cut(s)	555, 879, 1278, 2904
Hsp92I	GRCGYC	1 cut(s)	2508
HspAI	GCGC	1 cut(s)	650
Kpn2I	TCCGGA	2 cut(s)	1207, 2967
KpnI	GGTACC	1 cut(s)	1300
Ksp22I	TGATCA	2 cut(s)	1744, 1980
KspI	CCGCGG	1 cut(s)	1138
LmnI	GCTCC	8 cut(s)	355, 857, 1126, 1480, 1639, 1854, 2051, 2970
LweI	GCATC	7 cut(s)	16, 574, 661, 1201, 1216, 1549, 2275
MabI	ACCWGGT	1 cut(s)	951
MaeI	CTAG	9 cut(s)	332, 429, 1671, 1718, 2012, 2183, 2381, 2628, 2721
MaeII	ACGT	4 cut(s)	555, 879, 1278, 2904
MaeIII	GTNAC	4 cut(s)	1492, 2509, 2795, 2905
MbiI	CCGCTC	3 cut(s)	759, 1102, 3017
MfeI	CAATTG	1 cut(s)	1554
MflI	RGATCY	1 cut(s)	59
MhlI	GDGCHC	7 cut(s)	1131, 1398, 1816, 1859, 1976, 2238, 2720
MlsI	TGGCCA	2 cut(s)	721, 1887
MluNI	TGGCCA	2 cut(s)	721, 1887
MlyI	GAGTC	3 cut(s)	1518, 2166, 2902
MmeI	TCCRAC	5 cut(s)	289, 643, 1617, 2203, 2597
Mox20I	TGGCCA	2 cut(s)	721, 1887
Mph1103I	ATGCAT	2 cut(s)	1216, 2259
MroI	TCCGGA	2 cut(s)	1207, 2967
MroXI	GAANNNNTTC	2 cut(s)	155, 1203
MscI	TGGCCA	2 cut(s)	721, 1887
MseI	TTAA	2 cut(s)	224, 1256
MslI	CAYNNNNRTG	3 cut(s)	1224, 2013, 2603
Msp20I	TGGCCA	2 cut(s)	721, 1887
MspA1I	CMGCKG	7 cut(s)	524, 1137, 1466, 1486, 1602, 2049, 2891
MspI	CCGG	4 cut(s)	861, 1208, 2473, 2968
MspR9I	CCNGG	3 cut(s)	585, 644, 953
MunI	CAATTG	1 cut(s)	1554
Mva1269I	GAATGC	1 cut(s)	1803
MvaI	CCWGG	3 cut(s)	585, 644, 953
MvnI	CGCG	1 cut(s)	1137
MwoI	GCNNNNNNNGC	5 cut(s)	1199, 1774, 1854, 2254, 2263
NdeI	CATATG	1 cut(s)	2643
NlaIV	GGNNCC	9 cut(s)	146, 950, 1074, 1298, 1388, 1641, 1898, 2237, 2957
NmeAIII	GCCGAG	1 cut(s)	1874
NmuCI	GTSAC	3 cut(s)	2509, 2795, 2905
NsiI	ATGCAT	2 cut(s)	1216, 2259
NspI	RCATGY	4 cut(s)	1552, 2380, 2401, 2593
NspV	TTCGAA	1 cut(s)	2740
OliI	CACNNNNGTG	1 cut(s)	2013
PaeI	GCATGC	1 cut(s)	1552
PaeR7I	CTCGAG	1 cut(s)	1613
PagI	TCATGA	1 cut(s)	31
PceI	AGGCCT	1 cut(s)	2626
PciI	ACATGT	2 cut(s)	2397, 2589
PctI	GAATGC	1 cut(s)	1803
PdmI	GAANNNNTTC	2 cut(s)	155, 1203
PfeI	GAWTC	9 cut(s)	261, 335, 937, 978, 1429, 1724, 2060, 2193, 2390
PflMI	CCANNNNNTGG	2 cut(s)	707, 728
PleI	GAGTC	3 cut(s)	1517, 2165, 2902
PpsI	GAGTC	3 cut(s)	1517, 2165, 2902
Ppu21I	YACGTR	4 cut(s)	556, 880, 1279, 2905
PscI	ACATGT	2 cut(s)	2397, 2589
PshAI	GACNNNNGTC	1 cut(s)	2789
Psp124BI	GAGCTC	1 cut(s)	2720
Psp6I	CCWGG	3 cut(s)	583, 642, 951
PspFI	CCCAGC	2 cut(s)	848, 892
PspGI	CCWGG	3 cut(s)	583, 642, 951
PspN4I	GGNNCC	9 cut(s)	146, 950, 1074, 1298, 1388, 1641, 1898, 2237, 2957
PspPI	GGNCC	1 cut(s)	1897
PstI	CTGCAG	2 cut(s)	579, 2044
PsuI	RGATCY	1 cut(s)	59
PvuII	CAGCTG	4 cut(s)	524, 1466, 1486, 2891
RseI	CAYNNNNRTG	3 cut(s)	1224, 2013, 2603
SacI	GAGCTC	1 cut(s)	2720
SacII	CCGCGG	1 cut(s)	1138
SaqAI	TTAA	2 cut(s)	224, 1256
Sau96I	GGNCC	1 cut(s)	1897
ScaI	AGTACT	1 cut(s)	781
SchI	GAGTC	3 cut(s)	1518, 2166, 2902
ScrFI	CCNGG	3 cut(s)	585, 644, 953
SduI	GDGCHC	7 cut(s)	1131, 1398, 1816, 1859, 1976, 2238, 2720
SexAI	ACCWGGT	1 cut(s)	951
SfaNI	GCATC	7 cut(s)	16, 574, 661, 1201, 1216, 1549, 2275
SfcI	CTRYAG	4 cut(s)	575, 1230, 2040, 2144
Sfr274I	CTCGAG	1 cut(s)	1613
Sfr303I	CCGCGG	1 cut(s)	1138
SfuI	TTCGAA	1 cut(s)	2740
SgrBI	CCGCGG	1 cut(s)	1138
SinI	GGWCC	1 cut(s)	1897
SlaI	CTCGAG	1 cut(s)	1613
SmiMI	CAYNNNNRTG	3 cut(s)	1224, 2013, 2603
SmlI	CTYRAG	4 cut(s)	421, 609, 1613, 2950
SmoI	CTYRAG	4 cut(s)	421, 609, 1613, 2950
SnaBI	TACGTA	1 cut(s)	1279
SphI	GCATGC	1 cut(s)	1552
SseBI	AGGCCT	1 cut(s)	2626
SspI	AATATT	2 cut(s)	229, 1261
SspMI	CTAG	9 cut(s)	332, 429, 1671, 1718, 2012, 2183, 2381, 2628, 2721
SstI	GAGCTC	1 cut(s)	2720
StuI	AGGCCT	1 cut(s)	2626
StyD4I	CCNGG	3 cut(s)	583, 642, 951
StyI	CCWWGG	2 cut(s)	1269, 2011
TaaI	ACNGT	7 cut(s)	1498, 1871, 1978, 2278, 2518, 2674, 2788
TaiI	ACGT	4 cut(s)	558, 882, 1281, 2907
TaqII	GACCGA	2 cut(s)	990, 3038
TatI	WGTACW	4 cut(s)	76, 779, 2373, 2943
TauI	GCSGC	5 cut(s)	634, 1137, 1353, 1576, 2350
TfiI	GAWTC	9 cut(s)	261, 335, 937, 978, 1429, 1724, 2060, 2193, 2390
Tru1I	TTAA	2 cut(s)	224, 1256
Tru9I	TTAA	2 cut(s)	224, 1256
TscAI	CASTG	3 cut(s)	1118, 2283, 2521
TseFI	GTSAC	3 cut(s)	2509, 2795, 2905
Tsp45I	GTSAC	3 cut(s)	2509, 2795, 2905
TspGWI	ACGGA	5 cut(s)	753, 1238, 1889, 1959, 2901
TspRI	CASTG	3 cut(s)	1118, 2283, 2521
Van91I	CCANNNNNTGG	2 cut(s)	707, 728
VneI	GTGCAC	2 cut(s)	1812, 1972
VpaK11BI	GGWCC	1 cut(s)	1897
XapI	RAATTY	3 cut(s)	213, 1677, 2963
XbaI	TCTAGA	2 cut(s)	331, 2720
XceI	RCATGY	4 cut(s)	1552, 2380, 2401, 2593
XhoI	CTCGAG	1 cut(s)	1613
XmaJI	CCTAGG	1 cut(s)	2011
XmiI	GTMKAC	3 cut(s)	1275, 2117, 2586
XmnI	GAANNNNTTC	2 cut(s)	155, 1203
XspI	CTAG	9 cut(s)	332, 429, 1671, 1718, 2012, 2183, 2381, 2628, 2721
ZrmI	AGTACT	1 cut(s)	781
Zsp2I	ATGCAT	2 cut(s)	1216, 2259

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.