Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012016

Physical Location & Seq

Genomic Coordinates Reverse (-)

231648 .. 236246

Gene Structure 4599 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1003300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1797 bp

ATGGATGCGCTGAAGCTTTGGAGCCTATCCTCGCAGCCATTGAAGCACCTCTTCCGCGTTGCTTCATCCACCACCACGACGAATCTCAGACGACCCTTTTCTCATTCTTCTTCGTATTCTAGAGTTGTCCTCCGCTGTTGCTCTTCCACCTCCATCTCCACCACTCCCGCCAACGCCGCCAGAGCCGGCGGCCGCAACCGGCGGGCATCCACATCTTCCTCCACCTCCGATAGAGACGCTGTTCGAGCCATTCGTTTGAAGAAGGTAGAAGAACTGAGGAGCAAAGGTTTGGAGCCATATGCTTATGGGTGGGAAAGGACTCACACTGCTAATCAGCTGCAAGATATATACAAGGATTTAGCGGACGGTGAAGAGGCAAAAGCCGAGAGGGATGCTGTGTCTATTGCAGGAAGAATTGTGGCTCGCAGAGCGTTCGGAAAGCTTGCATTTTTGACTCTGAGGGACGATTCCGGCACAATTCAGCTTTACTGTGAAAAGGAACGGCTTGCAGACGAACAGTTCGATCAGTTAAAGGCATTTGTTGATATTGGTGATATAGTTGGTGCTAGTGGATCCATCAAACGGACAGAGAAAGGGGAGCTTTCAATTTATGTGAATTCTTTTGCAATTCTTACAAAATCTCTACTCCCGCTGCCGGACAAGTATCATGGTCTAACAGATGTGGACAAGCGCTACCGTCAGCGGTATGTAGATATGATTGCTAATCCTGAGGTAGCTGATGTATTCCGGAAAAGAGCAAAGATTATATCGGAGATACGAAAGGCAGTGGAGTCTGTTGGTTTCGTTGAGGTTGAAACTCCAGTTCTGCAGGGAGCAGCTGGTGGTGCAGAAGCTAGGCCATTTGTTACACACCATAATTCACTTGGAAGGGACCTGTACTTGAGAATAGCAACTGAGCTCCACTTAAAGAGAATGCTGGTTGGTGGGTTTGAAAAAGTGTATGAGATTGGTCGAATATTCAGGAATGAAGGCATTTCAACTCGTCATAATCCTGAATTTACCACTATAGAGATGTATGAAGCATATTCAGACTATGAAAGCATGATGAACATGGCGGAGGAAATTGTTACACGATGTGCGCTTGCAGTTCATGGGACTCTTACTGTTGATTATCAGGGGGTGGAGATACATCTTGAGCGGCCTTGGAGGAGGGAAACCATGCACAATCTTGTAAAAGAATCCGCTGAGATTGATTTCGGTGAGTTGAGCAATGATCTTAAAGTGGCTAAAGAAGTTACTCTGAAAACCCTCGGAGCTGACCTTGATCACAAAGACAAAGTTTCTATTGAAGCATGCACATCTATTGGCCACCTTCTTAATGAGGTTTTTGAGATTGCTGTAGAACCAAAGCTTCTGCAACCCACATTTGTATTAGACTATCCTATTGAGATATCTCCTCTGGCCAAGCCACACCGAAGAAATGCAGGGTTGACTGAGAGATTTGAGCTTTTCATCTGTGGTCGTGAGCTGGCCAATGCTTTCTCTGAACTGACCGATCCTATGGATCAGAGAGGACGCTTGGAAGAGCAAGTGAGGCAGCACAATGAGAAGAAAGCAGCAGCTATTTCCGAAACAGATGGTGCAAATGATAAAAGAAAGGAAAATGATGACTCGTATGAGGTGACTCTTGATGATGATTTCCTTACAGCTTTGGAATATGGGATGCCTCCAGCTTCAGGAATGGGACTTGGAATTGACAGGCTAGTCATGCTTTTAACAAACTCGGCTAGCATTAGAGATGTAATTGCCTTCCCAGTTCTTAAAGTTCAGCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0007275 GO:0008150 GO:0009507 GO:0009536 GO:0009791 GO:0009908 GO:0022414 GO:0032501 GO:0032502 GO:0035670 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0061458 GO:0090567 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_2 tRNA_anti-codon

Protein Analysis

599

Amino Acids

67.08

Weight (kDa)

6.08

Isoelectric Point (pI)

40.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA_anti-codon	PF01336	133 - 210	4.1e-18	OB-fold nucleic acid binding domain
tRNA-synt_2	PF00152	228 - 593	3.4e-84	tRNA synthetases class II (D, K and N)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012016)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13490
fragaria_vesca	FvH4_3g45770 FvH4_3g45770
malus_domestica	MD03G1003300.v1.1 MD11G1003100.v1.1
prunus_persica	Prupe.6G002900_v2.0.a1
pyrus_communis	pycom11g00280
rosa_chinensis	RchiOBHm_Chr5g0083841
rosa_laevigata	RLG00000037057
rosa_multiflora	Rmu_sc0001287.1_g000040 Rmu_sc0024007.1_g000006
rosa_roxburghii	Rroxscaffold_1G00000750
rosa_rugosa	Rorug05G0486400 Rorug05G0486500
rosa_samantha	Rh5AG539600 Rh5BG564700 Rh5CG586600 Rh5DG573200
rosa_wichuraiana	Rw5G050170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1724
AccBSI	CCGCTC	1 cut(s)	1159
AccII	CGCG	1 cut(s)	57
AccIII	TCCGGA	1 cut(s)	747
AclWI	GGATC	4 cut(s)	567, 580, 1511, 1533
AcoI	YGGCCR	4 cut(s)	190, 1327, 1422, 1491
AcsI	RAATTY	2 cut(s)	616, 1016
AcuI	CTGAAG	2 cut(s)	32, 1680
AdeI	CACNNNGTG	1 cut(s)	1097
AfaI	GTAC	1 cut(s)	899
AfeI	AGCGCT	1 cut(s)	692
AfiI	CCNNNNNNNGG	3 cut(s)	582, 655, 1697
AgsI	TTSAA	7 cut(s)	43, 259, 606, 815, 953, 999, 1310
Alw21I	GWGCWC	1 cut(s)	921
Alw26I	GTCTC	1 cut(s)	228
AlwI	GGATC	4 cut(s)	567, 580, 1511, 1533
Aor13HI	TCCGGA	1 cut(s)	747
Aor51HI	AGCGCT	1 cut(s)	692
AoxI	GGCC	6 cut(s)	190, 857, 1160, 1327, 1422, 1491
ApeKI	GCWGC	7 cut(s)	34, 337, 652, 836, 1558, 1577, 1580
ApoI	RAATTY	2 cut(s)	616, 1016
ArsI	GACNNNNNNTTYG	2 cut(s)	503, 535
AspLEI	GCGC	3 cut(s)	10, 693, 1102
AspS9I	GGNCC	1 cut(s)	892
AsuHPI	GGTGA	4 cut(s)	380, 563, 1232, 1654
AsuNHI	GCTAGC	1 cut(s)	1748
AvaII	GGWCC	1 cut(s)	892
AxyI	CCTNAGG	1 cut(s)	729
BalI	TGGCCA	3 cut(s)	1329, 1424, 1493
BamHI	GGATCC	1 cut(s)	572
BanII	GRGCYC	1 cut(s)	921
Bbv12I	GWGCWC	1 cut(s)	921
BbvI	GCAGC	7 cut(s)	46, 324, 639, 848, 1570, 1589, 1592
BccI	CCATC	3 cut(s)	161, 584, 1592
BceAI	ACGGC	1 cut(s)	518
BcgI	CGANNNNNNTGC	4 cut(s)	374, 408, 415, 449
BclI	TGATCA	1 cut(s)	1285
BcoDI	GTCTC	1 cut(s)	228
BfaI	CTAG	5 cut(s)	120, 567, 855, 1724, 1749
BfmI	CTRYAG	3 cut(s)	827, 1026, 1359
BfoI	RGCGCY	1 cut(s)	694
Bme18I	GGWCC	1 cut(s)	892
BmgT120I	GGNCC	1 cut(s)	892
BmiI	GGNNCC	4 cut(s)	23, 294, 574, 893
BmrI	ACTGGG	1 cut(s)	1769
BmsI	GCATC	3 cut(s)	215, 382, 1674
BmtI	GCTAGC	1 cut(s)	1752
BmuI	ACTGGG	1 cut(s)	1769
BplI	GAGNNNNNCTC	2 cut(s)	114, 146
BpmI	CTGGAG	2 cut(s)	804, 1674
BpuEI	CTTGAG	2 cut(s)	922, 1175
BsaJI	CCNNGG	2 cut(s)	1163, 1270
BsaWI	WCCGGW	1 cut(s)	747
BsaXI	ACNNNNNCTCC	2 cut(s)	271, 301
Bsc4I	CCNNNNNNNGG	3 cut(s)	582, 655, 1697
Bse118I	RCCGGY	2 cut(s)	185, 198
Bse1I	ACTGG	2 cut(s)	821, 1775
Bse21I	CCTNAGG	1 cut(s)	729
Bse3DI	GCAATG	1 cut(s)	1237
BseAI	TCCGGA	1 cut(s)	747
BseDI	CCNNGG	2 cut(s)	1163, 1270
BseGI	GGATG	5 cut(s)	10, 65, 206, 397, 1689
BseLI	CCNNNNNNNGG	3 cut(s)	582, 655, 1697
BseMI	GCAATG	1 cut(s)	1237
BseMII	CTCAG	7 cut(s)	100, 266, 449, 720, 906, 1197, 1446
BseNI	ACTGG	2 cut(s)	821, 1775
BseRI	GAGGAG	3 cut(s)	292, 1183, 1407
BseX3I	CGGCCG	1 cut(s)	190
BseXI	GCAGC	7 cut(s)	46, 324, 639, 848, 1570, 1589, 1592
BsgI	GTGCAG	1 cut(s)	867
Bsh1236I	CGCG	1 cut(s)	57
Bsh1285I	CGRYCG	1 cut(s)	193
BshFI	GGCC	6 cut(s)	192, 859, 1162, 1329, 1424, 1493
BsiEI	CGRYCG	1 cut(s)	193
BsiHKAI	GWGCWC	1 cut(s)	921
BsiSI	CCGG	5 cut(s)	186, 199, 471, 656, 748
BslFI	GGGAC	4 cut(s)	476, 905, 1129, 1719
BslI	CCNNNNNNNGG	3 cut(s)	582, 655, 1697
BsmAI	GTCTC	1 cut(s)	228
BsmBI	CGTCTC	1 cut(s)	228
BsmFI	GGGAC	4 cut(s)	476, 905, 1129, 1719
BsmI	GAATGC	1 cut(s)	939
BsnI	GGCC	6 cut(s)	192, 859, 1162, 1329, 1424, 1493
Bsp1286I	GDGCHC	1 cut(s)	921
Bsp13I	TCCGGA	1 cut(s)	747
Bsp143I	GATC	6 cut(s)	523, 572, 1234, 1285, 1516, 1525
BspANI	GGCC	6 cut(s)	192, 859, 1162, 1329, 1424, 1493
BspCNI	CTCAG	7 cut(s)	99, 267, 450, 721, 907, 1198, 1447
BspEI	TCCGGA	1 cut(s)	747
BspFNI	CGCG	1 cut(s)	57
BspLI	GGNNCC	4 cut(s)	23, 294, 574, 893
BspMAI	CTGCAG	1 cut(s)	831
BspOI	GCTAGC	1 cut(s)	1752
BspPI	GGATC	4 cut(s)	567, 580, 1511, 1533
BspQI	GCTCTTC	2 cut(s)	148, 1539
BsrBI	CCGCTC	1 cut(s)	1159
BsrDI	GCAATG	1 cut(s)	1237
BsrFI	RCCGGY	2 cut(s)	185, 198
BsrI	ACTGG	2 cut(s)	821, 1775
BssAI	RCCGGY	2 cut(s)	185, 198
BssECI	CCNNGG	2 cut(s)	1163, 1270
BssMI	GATC	6 cut(s)	523, 572, 1234, 1285, 1516, 1525
BssT1I	CCWWGG	1 cut(s)	1163
Bst4CI	ACNGT	5 cut(s)	368, 491, 519, 698, 1126
Bst6I	CTCTTC	4 cut(s)	56, 148, 366, 1539
BstC8I	GCNNGC	9 cut(s)	187, 204, 424, 444, 507, 1104, 1315, 1491, 1750
BstDEI	CTNAG	7 cut(s)	86, 275, 458, 729, 915, 1206, 1455
BstF5I	GGATG	5 cut(s)	10, 65, 206, 397, 1689
BstFNI	CGCG	1 cut(s)	57
BstH2I	RGCGCY	1 cut(s)	694
BstHHI	GCGC	3 cut(s)	10, 693, 1102
BstKTI	GATC	6 cut(s)	526, 575, 1237, 1288, 1519, 1528
BstMAI	GTCTC	1 cut(s)	228
BstMBI	GATC	6 cut(s)	523, 572, 1234, 1285, 1516, 1525
BstMCI	CGRYCG	1 cut(s)	193
BstMWI	GCNNNNNNNGC	8 cut(s)	43, 176, 182, 245, 428, 845, 1555, 1729
BstNSI	RCATGY	1 cut(s)	1317
BstSFI	CTRYAG	3 cut(s)	827, 1026, 1359
BstUI	CGCG	1 cut(s)	57
BstV1I	GCAGC	7 cut(s)	46, 324, 639, 848, 1570, 1589, 1592
BstX2I	RGATCY	1 cut(s)	572
BstYI	RGATCY	1 cut(s)	572
BstZI	CGGCCG	1 cut(s)	190
Bsu36I	CCTNAGG	1 cut(s)	729
BsuRI	GGCC	6 cut(s)	192, 859, 1162, 1329, 1424, 1493
BtsCI	GGATG	5 cut(s)	10, 65, 206, 397, 1689
BtsI	GCAGTG	2 cut(s)	324, 792
BtsIMutI	CAGTG	2 cut(s)	324, 792
Cac8I	GCNNGC	9 cut(s)	187, 204, 424, 444, 507, 1104, 1315, 1491, 1750
CciNI	GCGGCCGC	1 cut(s)	190
CfoI	GCGC	3 cut(s)	10, 693, 1102
Cfr10I	RCCGGY	2 cut(s)	185, 198
Cfr13I	GGNCC	1 cut(s)	892
CseI	GACGC	2 cut(s)	245, 1545
Csp6I	GTAC	1 cut(s)	898
CviAII	CATG	7 cut(s)	668, 1063, 1072, 1112, 1180, 1314, 1729
CviQI	GTAC	1 cut(s)	898
DdeI	CTNAG	7 cut(s)	86, 275, 458, 729, 915, 1206, 1455
DpnI	GATC	6 cut(s)	525, 574, 1236, 1287, 1518, 1527
DpnII	GATC	6 cut(s)	523, 572, 1234, 1285, 1516, 1525
DraIII	CACNNNGTG	1 cut(s)	1097
DrdI	GACNNNNNNGTC	1 cut(s)	1724
DseDI	GACNNNNNNGTC	1 cut(s)	1724
EaeI	YGGCCR	4 cut(s)	190, 1327, 1422, 1491
EagI	CGGCCG	1 cut(s)	190
Eam1104I	CTCTTC	4 cut(s)	56, 148, 366, 1539
EarI	CTCTTC	4 cut(s)	56, 148, 366, 1539
EciI	GGCGGA	1 cut(s)	1091
Ecl136II	GAGCTC	1 cut(s)	919
EclXI	CGGCCG	1 cut(s)	190
Eco130I	CCWWGG	1 cut(s)	1163
Eco24I	GRGCYC	1 cut(s)	921
Eco32I	GATATC	1 cut(s)	1413
Eco47I	GGWCC	1 cut(s)	892
Eco47III	AGCGCT	1 cut(s)	692
Eco52I	CGGCCG	1 cut(s)	190
Eco53kI	GAGCTC	1 cut(s)	919
Eco57I	CTGAAG	2 cut(s)	32, 1680
Eco81I	CCTNAGG	1 cut(s)	729
EcoICRI	GAGCTC	1 cut(s)	919
EcoO109I	RGGNCCY	1 cut(s)	892
EcoRI	GAATTC	1 cut(s)	616
EcoRV	GATATC	1 cut(s)	1413
EcoT14I	CCWWGG	1 cut(s)	1163
EcoT38I	GRGCYC	1 cut(s)	921
ErhI	CCWWGG	1 cut(s)	1163
Esp3I	CGTCTC	1 cut(s)	228
FaeI	CATG	7 cut(s)	671, 1066, 1075, 1115, 1183, 1317, 1732
FalI	AAGNNNNNCTT	4 cut(s)	35, 67, 585, 617
FaqI	GGGAC	4 cut(s)	476, 905, 1129, 1719
FatI	CATG	7 cut(s)	667, 1062, 1071, 1111, 1179, 1313, 1728
FauI	CCCGC	3 cut(s)	175, 195, 657
FauNDI	CATATG	1 cut(s)	298
FbaI	TGATCA	1 cut(s)	1285
FokI	GGATG	5 cut(s)	17, 52, 193, 404, 1696
FriOI	GRGCYC	1 cut(s)	921
FspBI	CTAG	5 cut(s)	120, 567, 855, 1724, 1749
GlaI	GCGC	3 cut(s)	9, 692, 1101
GsuI	CTGGAG	2 cut(s)	804, 1674
HaeII	RGCGCY	1 cut(s)	694
HaeIII	GGCC	6 cut(s)	192, 859, 1162, 1329, 1424, 1493
HapII	CCGG	5 cut(s)	186, 199, 471, 656, 748
HgaI	GACGC	2 cut(s)	245, 1545
HhaI	GCGC	3 cut(s)	10, 693, 1102
Hin1II	CATG	7 cut(s)	671, 1066, 1075, 1115, 1183, 1317, 1732
Hin6I	GCGC	3 cut(s)	8, 691, 1100
HinP1I	GCGC	3 cut(s)	8, 691, 1100
HincII	GTYRAC	1 cut(s)	1452
HindII	GTYRAC	1 cut(s)	1452
HindIII	AAGCTT	3 cut(s)	14, 440, 1370
HinfI	GANTC	9 cut(s)	82, 319, 454, 467, 791, 1117, 1199, 1631, 1645
HpaII	CCGG	5 cut(s)	186, 199, 471, 656, 748
HphI	GGTGA	4 cut(s)	380, 563, 1232, 1654
Hpy166II	GTNNAC	2 cut(s)	685, 1452
Hpy188III	TCNNGA	9 cut(s)	120, 728, 748, 982, 1013, 1154, 1484, 1649, 1698
Hpy8I	GTNNAC	2 cut(s)	685, 1452
Hpy99I	CGWCG	1 cut(s)	82
HpyAV	CCTTC	5 cut(s)	256, 882, 983, 1343, 1780
HpyCH4III	ACNGT	5 cut(s)	368, 491, 519, 698, 1126
HpyF10VI	GCNNNNNNNGC	8 cut(s)	43, 176, 182, 245, 428, 845, 1555, 1729
HpyF3I	CTNAG	7 cut(s)	86, 275, 458, 729, 915, 1206, 1455
Hsp92II	CATG	7 cut(s)	671, 1066, 1075, 1115, 1183, 1317, 1732
HspAI	GCGC	3 cut(s)	8, 691, 1100
Kpn2I	TCCGGA	1 cut(s)	747
KroI	GCCGGC	1 cut(s)	185
KroNI	GCCGGC	1 cut(s)	187
Ksp22I	TGATCA	1 cut(s)	1285
Kzo9I	GATC	6 cut(s)	523, 572, 1234, 1285, 1516, 1525
LguI	GCTCTTC	2 cut(s)	148, 1539
LmnI	GCTCC	7 cut(s)	21, 279, 292, 598, 833, 924, 1274
Lsp1109I	GCAGC	7 cut(s)	46, 324, 639, 848, 1570, 1589, 1592
LweI	GCATC	3 cut(s)	215, 382, 1674
MaeI	CTAG	5 cut(s)	120, 567, 855, 1724, 1749
MaeIII	GTNAC	4 cut(s)	865, 1087, 1255, 1642
MalI	GATC	6 cut(s)	525, 574, 1236, 1287, 1518, 1527
MbiI	CCGCTC	1 cut(s)	1159
MboI	GATC	6 cut(s)	523, 572, 1234, 1285, 1516, 1525
MflI	RGATCY	1 cut(s)	572
MhlI	GDGCHC	1 cut(s)	921
MlsI	TGGCCA	3 cut(s)	1329, 1424, 1493
MluNI	TGGCCA	3 cut(s)	1329, 1424, 1493
MlyI	GAGTC	6 cut(s)	313, 448, 800, 1111, 1625, 1639
Mox20I	TGGCCA	3 cut(s)	1329, 1424, 1493
MroI	TCCGGA	1 cut(s)	747
MroNI	GCCGGC	1 cut(s)	185
MscI	TGGCCA	3 cut(s)	1329, 1424, 1493
MseI	TTAA	6 cut(s)	530, 926, 1239, 1338, 1736, 1782
Msp20I	TGGCCA	3 cut(s)	1329, 1424, 1493
MspA1I	CMGCKG	6 cut(s)	135, 337, 652, 703, 839, 1205
MspI	CCGG	5 cut(s)	186, 199, 471, 656, 748
Mva1269I	GAATGC	1 cut(s)	939
MvnI	CGCG	1 cut(s)	57
MwoI	GCNNNNNNNGC	8 cut(s)	43, 176, 182, 245, 428, 845, 1555, 1729
NaeI	GCCGGC	1 cut(s)	187
NdeI	CATATG	1 cut(s)	298
NdeII	GATC	6 cut(s)	523, 572, 1234, 1285, 1516, 1525
NgoMIV	GCCGGC	1 cut(s)	185
NheI	GCTAGC	1 cut(s)	1748
NlaIII	CATG	7 cut(s)	671, 1066, 1075, 1115, 1183, 1317, 1732
NlaIV	GGNNCC	4 cut(s)	23, 294, 574, 893
NmeAIII	GCCGAG	2 cut(s)	409, 1724
NmuCI	GTSAC	1 cut(s)	1642
NotI	GCGGCCGC	1 cut(s)	190
NspI	RCATGY	1 cut(s)	1317
PaeI	GCATGC	1 cut(s)	1317
PciSI	GCTCTTC	2 cut(s)	148, 1539
PcsI	WCGNNNNNNNCGW	2 cut(s)	250, 519
PctI	GAATGC	1 cut(s)	939
PdiI	GCCGGC	1 cut(s)	187
PfeI	GAWTC	3 cut(s)	82, 467, 1199
PleI	GAGTC	6 cut(s)	313, 448, 799, 1111, 1625, 1639
PpsI	GAGTC	6 cut(s)	313, 448, 799, 1111, 1625, 1639
PpuMI	RGGWCCY	1 cut(s)	892
Psp124BI	GAGCTC	1 cut(s)	921
Psp5II	RGGWCCY	1 cut(s)	892
PspN4I	GGNNCC	4 cut(s)	23, 294, 574, 893
PspPI	GGNCC	1 cut(s)	892
PspPPI	RGGWCCY	1 cut(s)	892
PstI	CTGCAG	1 cut(s)	831
PsuI	RGATCY	1 cut(s)	572
PvuII	CAGCTG	2 cut(s)	337, 839
RsaI	GTAC	1 cut(s)	899
RsaNI	GTAC	1 cut(s)	898
SacI	GAGCTC	1 cut(s)	921
SapI	GCTCTTC	2 cut(s)	148, 1539
SaqAI	TTAA	6 cut(s)	530, 926, 1239, 1338, 1736, 1782
Sau3AI	GATC	6 cut(s)	523, 572, 1234, 1285, 1516, 1525
Sau96I	GGNCC	1 cut(s)	892
SchI	GAGTC	6 cut(s)	313, 448, 800, 1111, 1625, 1639
SduI	GDGCHC	1 cut(s)	921
SfaNI	GCATC	3 cut(s)	215, 382, 1674
SfcI	CTRYAG	3 cut(s)	827, 1026, 1359
SinI	GGWCC	1 cut(s)	892
SmlI	CTYRAG	2 cut(s)	901, 1154
SmoI	CTYRAG	2 cut(s)	901, 1154
SphI	GCATGC	1 cut(s)	1317
SspI	AATATT	1 cut(s)	978
SspMI	CTAG	5 cut(s)	120, 567, 855, 1724, 1749
SstI	GAGCTC	1 cut(s)	921
StyI	CCWWGG	1 cut(s)	1163
TaaI	ACNGT	5 cut(s)	368, 491, 519, 698, 1126
TaqI	TCGA	3 cut(s)	244, 522, 973
TaqII	GACCGA	1 cut(s)	1529
TatI	WGTACW	1 cut(s)	897
TauI	GCSGC	4 cut(s)	179, 192, 195, 1162
TfiI	GAWTC	3 cut(s)	82, 467, 1199
Tru1I	TTAA	6 cut(s)	530, 926, 1239, 1338, 1736, 1782
Tru9I	TTAA	6 cut(s)	530, 926, 1239, 1338, 1736, 1782
TscAI	CASTG	2 cut(s)	331, 792
TseFI	GTSAC	1 cut(s)	1642
TseI	GCWGC	7 cut(s)	34, 337, 652, 836, 1558, 1577, 1580
Tsp45I	GTSAC	1 cut(s)	1642
TspDTI	ATGAA	7 cut(s)	54, 1002, 1053, 1071, 1082, 1100, 1462
TspGWI	ACGGA	1 cut(s)	598
TspRI	CASTG	2 cut(s)	331, 792
VpaK11BI	GGWCC	1 cut(s)	892
XapI	RAATTY	2 cut(s)	616, 1016
XbaI	TCTAGA	1 cut(s)	119
XceI	RCATGY	1 cut(s)	1317
XcmI	CCANNNNNNNNNTGG	1 cut(s)	881
XspI	CTAG	5 cut(s)	120, 567, 855, 1724, 1749

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.