Rorug05G0486500

Lysyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012016
Physical Location & Seq
Reverse (-)
67009572 .. 67010578
1007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0486500.1

Sequence Viewer

Length: 861 bp
ATGGCTTCCTTCAACTACCCTGACGAATCTCCAGAACAATTGATACAGAGTCTGAAATCGGAAGGAAAGCTCGGCTGCTTTCTCCGTCGAGACCAAATGTCAAAGAAGCCAGTGTACGTAGAACTCGACGTTCAAAAATTCTGGAACACCGTCAACGACACCCTCACCGCAGTGATTTCCCGCGCTCTTAACTTGAGCCCCGAATTCCAAAAACTCGACCATATTAAGCAACAAGCCACCTACTTCGCCACTGAAATCACAAAGGGAGTCTGCGTTCTTACTTATCTCAAGCTAAGAGCTGTTAATTTCTCTCTCGACTTCGGCCTAGGCGCTTCTAGATTCTTCAAGAAGCCGATGGTTCATTCCCCCTTTGAGGTCCCACAACCATTTGGGGTTGCTATCTCTGGACTAGGGTTTGTACGAGTCACCAGTCTGCCGTTTGAGCAGATCATATGCCCCACTCTTTCGGAGGTTAACGCCACCGGGCTTTGCTTGCCGTCGGATCAAAGCTGGTCGTTTCATAGATATTCTCAAGCTGTGGAATTTGCAAAAGGGATTGGTCTTAAGTTTTCGACCGTTGATTTGTCTGTGAACTTAGGTTCGAGCTGGTGGCTGTACCGGCCGGTTGTGGATGGAGGCATCTTTCGACTGCAGTGTATCCTACCTCCAGATAACTACAGTAAAGAGAGTGCGGTGGTGAGAACTTTGTTTATGAGTTCGAGTGTGGATAATATTGCCGGACACGAGATCTTTGATCTGTCCAGTGTCGAGAACCATGACTATGGTTCTATGCTTCGAAATCCACACGAAGGCATCAACGTTTCCACTTATCTTGCATTTGAAGAAGTTGCTGAGAATTGA

Protein Analysis

286

Amino Acids

32.04

Weight (kDa)

5.71

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13490
fragaria_vesca FvH4_3g45770 FvH4_3g45770
malus_domestica MD03G1003300.v1.1 MD11G1003100.v1.1
prunus_persica Prupe.6G002900_v2.0.a1
pyrus_communis pycom11g00280
rosa_chinensis RchiOBHm_Chr5g0083841
rosa_laevigata RLG00000037057
rosa_multiflora Rmu_sc0001287.1_g000040 Rmu_sc0024007.1_g000006
rosa_roxburghii Rroxscaffold_1G00000750
rosa_rugosa Rorug05G0486400 Rorug05G0486500
rosa_samantha Rh5AG539600 Rh5BG564700 Rh5CG586600 Rh5DG573200
rosa_wichuraiana Rw5G050170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 183
AciI CCGC 3 cut(s) 168, 181, 692
AclI AACGTT 1 cut(s) 819
AclWI GGATC 1 cut(s) 510
AcoI YGGCCR 1 cut(s) 620
AcsI RAATTY 3 cut(s) 137, 203, 542
AfaI GTAC 3 cut(s) 116, 420, 617
AfiI CCNNNNNNNGG 2 cut(s) 373, 809
AflII CTTAAG 1 cut(s) 563
AgsI TTSAA 4 cut(s) 13, 134, 346, 842
AhdI GACNNNNNGTC 1 cut(s) 97
AleI CACNNNNGTG 1 cut(s) 170
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 6 cut(s) 70, 292, 299, 510, 536, 606
AluI AGCT 6 cut(s) 70, 292, 299, 510, 536, 606
Alw26I GTCTC 1 cut(s) 84
AlwI GGATC 1 cut(s) 510
AlwNI CAGNNNCTG 1 cut(s) 52
AoxI GGCC 2 cut(s) 322, 620
ApeKI GCWGC 1 cut(s) 75
ApoI RAATTY 3 cut(s) 137, 203, 542
ArsI GACNNNNNNTTYG 2 cut(s) 565, 597
AspA2I CCTAGG 1 cut(s) 325
AspLEI GCGC 2 cut(s) 185, 332
AspS9I GGNCC 1 cut(s) 376
AsuC2I CCSGG 1 cut(s) 484
AsuHPI GGTGA 3 cut(s) 157, 418, 709
AsuII TTCGAA 1 cut(s) 796
AvaII GGWCC 1 cut(s) 376
AvrII CCTAGG 1 cut(s) 325
BanII GRGCYC 1 cut(s) 200
BarI GAAGNNNNNNTAC 2 cut(s) 98, 130
BauI CACGAG 1 cut(s) 743
BbvI GCAGC 1 cut(s) 62
BccI CCATC 2 cut(s) 349, 626
BceAI ACGGC 2 cut(s) 421, 481
BciVI GTATCC 1 cut(s) 668
BcnI CCSGG 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 84
BfaI CTAG 3 cut(s) 326, 336, 410
BfmI CTRYAG 2 cut(s) 650, 676
BfoI RGCGCY 1 cut(s) 333
BfrI CTTAAG 1 cut(s) 563
BfuI GTATCC 1 cut(s) 668
BglII AGATCT 1 cut(s) 747
BisI GCNGC 1 cut(s) 76
BlnI CCTAGG 1 cut(s) 325
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 1 cut(s) 484
Bme18I GGWCC 1 cut(s) 376
BmeRI GACNNNNNGTC 1 cut(s) 97
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 378
BmrFI CCNGG 1 cut(s) 484
BmsI GCATC 2 cut(s) 648, 822
BpmI CTGGAG 2 cut(s) 15, 651
Bpu14I TTCGAA 1 cut(s) 796
BpuEI CTTGAG 3 cut(s) 214, 272, 516
BpuMI CCSGG 1 cut(s) 484
BsaAI YACGTR 1 cut(s) 118
BsaI GGTCTC 1 cut(s) 84
BsaJI CCNNGG 1 cut(s) 325
BsaXI ACNNNNNCTCC 2 cut(s) 258, 288
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 809
Bse118I RCCGGY 2 cut(s) 618, 622
Bse1I ACTGG 3 cut(s) 110, 429, 762
BseDI CCNNGG 1 cut(s) 325
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 2 cut(s) 373, 809
BseMII CTCAG 1 cut(s) 843
BseNI ACTGG 3 cut(s) 110, 429, 762
BseX3I CGGCCG 1 cut(s) 620
BseXI GCAGC 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 183
Bsh1285I CGRYCG 2 cut(s) 576, 623
BshFI GGCC 2 cut(s) 324, 622
BsiEI CGRYCG 2 cut(s) 576, 623
BsiSI CCGG 4 cut(s) 483, 619, 623, 738
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 2 cut(s) 373, 809
BsmAI GTCTC 1 cut(s) 84
BsmFI GGGAC 1 cut(s) 362
BsnI GGCC 2 cut(s) 324, 622
Bso31I GGTCTC 1 cut(s) 84
Bsp119I TTCGAA 1 cut(s) 796
Bsp1286I GDGCHC 1 cut(s) 200
Bsp143I GATC 4 cut(s) 447, 502, 747, 754
BspACI CCGC 3 cut(s) 168, 181, 692
BspANI GGCC 2 cut(s) 324, 622
BspCNI CTCAG 1 cut(s) 844
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 378
BspMAI CTGCAG 1 cut(s) 654
BspPI GGATC 1 cut(s) 510
BspT104I TTCGAA 1 cut(s) 796
BspTI CTTAAG 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 84
BsrFI RCCGGY 2 cut(s) 618, 622
BsrI ACTGG 3 cut(s) 110, 429, 762
BssAI RCCGGY 2 cut(s) 618, 622
BssECI CCNNGG 1 cut(s) 325
BssMI GATC 4 cut(s) 447, 502, 747, 754
BssSI CACGAG 1 cut(s) 743
BssT1I CCWWGG 1 cut(s) 325
Bst2BI CACGAG 1 cut(s) 743
Bst4CI ACNGT 3 cut(s) 151, 577, 680
BstAFI CTTAAG 1 cut(s) 563
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 1 cut(s) 796
BstC8I GCNNGC 1 cut(s) 494
BstDEI CTNAG 3 cut(s) 293, 595, 852
BstF5I GGATG 1 cut(s) 637
BstFNI CGCG 1 cut(s) 183
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 2 cut(s) 185, 332
BstKTI GATC 4 cut(s) 450, 505, 750, 757
BstMAI GTCTC 1 cut(s) 84
BstMBI GATC 4 cut(s) 447, 502, 747, 754
BstMCI CGRYCG 2 cut(s) 576, 623
BstMWI GCNNNNNNNGC 3 cut(s) 442, 493, 619
BstSCI CCNGG 1 cut(s) 482
BstSFI CTRYAG 2 cut(s) 650, 676
BstSNI TACGTA 1 cut(s) 118
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 62
BstX2I RGATCY 1 cut(s) 747
BstXI CCANNNNNNTGG 1 cut(s) 782
BstYI RGATCY 1 cut(s) 747
BstZI CGGCCG 1 cut(s) 620
BsuI GTATCC 1 cut(s) 668
BsuRI GGCC 2 cut(s) 324, 622
BtsCI GGATG 1 cut(s) 637
BtsI GCAGTG 2 cut(s) 177, 659
BtsIMutI CAGTG 5 cut(s) 117, 177, 249, 659, 769
Cac8I GCNNGC 1 cut(s) 494
CaiI CAGNNNCTG 1 cut(s) 52
CfoI GCGC 2 cut(s) 185, 332
Cfr10I RCCGGY 2 cut(s) 618, 622
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 3 cut(s) 115, 419, 616
CspCI CAANNNNNGTGG 2 cut(s) 814, 849
CviAII CATG 1 cut(s) 776
CviQI GTAC 3 cut(s) 115, 419, 616
DdeI CTNAG 3 cut(s) 293, 595, 852
DpnI GATC 4 cut(s) 449, 504, 749, 756
DpnII GATC 4 cut(s) 447, 502, 747, 754
DriI GACNNNNNGTC 1 cut(s) 97
EaeI YGGCCR 1 cut(s) 620
EagI CGGCCG 1 cut(s) 620
Eam1105I GACNNNNNGTC 1 cut(s) 97
EclXI CGGCCG 1 cut(s) 620
Eco105I TACGTA 1 cut(s) 118
Eco130I CCWWGG 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 200
Eco31I GGTCTC 1 cut(s) 84
Eco47I GGWCC 1 cut(s) 376
Eco52I CGGCCG 1 cut(s) 620
EcoO109I RGGNCCY 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 203
EcoT14I CCWWGG 1 cut(s) 325
EcoT38I GRGCYC 1 cut(s) 200
ErhI CCWWGG 1 cut(s) 325
FaeI CATG 1 cut(s) 779
FaiI YATR 8 cut(s) 222, 452, 454, 522, 713, 777, 783, 791
FaqI GGGAC 1 cut(s) 362
FatI CATG 1 cut(s) 775
FauI CCCGC 1 cut(s) 188
FauNDI CATATG 1 cut(s) 452
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 1 cut(s) 644
FriOI GRGCYC 1 cut(s) 200
Fsp4HI GCNGC 1 cut(s) 76
FspBI CTAG 3 cut(s) 326, 336, 410
GlaI GCGC 2 cut(s) 184, 331
GluI GCNGC 1 cut(s) 76
GsuI CTGGAG 2 cut(s) 15, 651
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 2 cut(s) 324, 622
HapII CCGG 4 cut(s) 483, 619, 623, 738
HhaI GCGC 2 cut(s) 185, 332
Hin1II CATG 1 cut(s) 779
Hin6I GCGC 2 cut(s) 183, 330
HinP1I GCGC 2 cut(s) 183, 330
HincII GTYRAC 2 cut(s) 154, 475
HindII GTYRAC 2 cut(s) 154, 475
HinfI GANTC 5 cut(s) 26, 49, 267, 339, 423
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 4 cut(s) 483, 619, 623, 738
HphI GGTGA 3 cut(s) 157, 418, 709
Hpy166II GTNNAC 4 cut(s) 115, 154, 475, 592
Hpy188I TCNGA 4 cut(s) 54, 61, 469, 502
Hpy188III TCNNGA 9 cut(s) 32, 89, 142, 314, 336, 346, 405, 668, 769
Hpy8I GTNNAC 4 cut(s) 115, 154, 475, 592
Hpy99I CGWCG 3 cut(s) 90, 131, 502
HpyAV CCTTC 3 cut(s) 19, 56, 803
HpyCH4III ACNGT 3 cut(s) 151, 577, 680
HpyCH4IV ACGT 3 cut(s) 117, 129, 819
HpyCH4V TGCA 3 cut(s) 548, 652, 836
HpyF10VI GCNNNNNNNGC 3 cut(s) 442, 493, 619
HpyF3I CTNAG 3 cut(s) 293, 595, 852
HpySE526I ACGT 3 cut(s) 117, 129, 819
Hsp92II CATG 1 cut(s) 779
HspAI GCGC 2 cut(s) 183, 330
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 4 cut(s) 447, 502, 747, 754
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 2 cut(s) 648, 822
MaeI CTAG 3 cut(s) 326, 336, 410
MaeII ACGT 3 cut(s) 117, 129, 819
MaeIII GTNAC 1 cut(s) 424
MalI GATC 4 cut(s) 449, 504, 749, 756
MboI GATC 4 cut(s) 447, 502, 747, 754
MboII GAAGA 2 cut(s) 334, 854
MfeI CAATTG 1 cut(s) 38
MflI RGATCY 1 cut(s) 747
MhlI GDGCHC 1 cut(s) 200
MluCI AATT 6 cut(s) 38, 137, 203, 304, 542, 856
MlyI GAGTC 3 cut(s) 58, 276, 432
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 5 cut(s) 173, 367, 463, 629, 675
MseI TTAA 5 cut(s) 189, 225, 303, 474, 564
MslI CAYNNNNRTG 2 cut(s) 170, 780
MspCI CTTAAG 1 cut(s) 563
MspI CCGG 4 cut(s) 483, 619, 623, 738
MspR9I CCNGG 1 cut(s) 484
MunI CAATTG 1 cut(s) 38
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 3 cut(s) 442, 493, 619
NciI CCSGG 1 cut(s) 484
NdeI CATATG 1 cut(s) 452
NdeII GATC 4 cut(s) 447, 502, 747, 754
NlaIII CATG 1 cut(s) 779
NlaIV GGNNCC 1 cut(s) 378
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 424
NspV TTCGAA 1 cut(s) 796
OliI CACNNNNGTG 1 cut(s) 170
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 2 cut(s) 26, 339
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 3 cut(s) 57, 275, 431
PpsI GAGTC 3 cut(s) 57, 275, 431
Ppu21I YACGTR 1 cut(s) 118
PpuMI RGGWCCY 1 cut(s) 376
Psp1406I AACGTT 1 cut(s) 819
Psp5II RGGWCCY 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 378
PspPI GGNCC 1 cut(s) 376
PspPPI RGGWCCY 1 cut(s) 376
PsrI GAACNNNNNNTAC 2 cut(s) 27, 59
PstI CTGCAG 1 cut(s) 654
PstNI CAGNNNCTG 1 cut(s) 52
PsuI RGATCY 1 cut(s) 747
RsaI GTAC 3 cut(s) 116, 420, 617
RsaNI GTAC 3 cut(s) 115, 419, 616
RseI CAYNNNNRTG 2 cut(s) 170, 780
SaqAI TTAA 5 cut(s) 189, 225, 303, 474, 564
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 4 cut(s) 447, 502, 747, 754
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 3 cut(s) 58, 276, 432
ScrFI CCNGG 1 cut(s) 484
SduI GDGCHC 1 cut(s) 200
SfaNI GCATC 2 cut(s) 648, 822
SfcI CTRYAG 2 cut(s) 650, 676
SfuI TTCGAA 1 cut(s) 796
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 170, 780
SmlI CTYRAG 4 cut(s) 193, 287, 531, 563
SmoI CTYRAG 4 cut(s) 193, 287, 531, 563
SnaBI TACGTA 1 cut(s) 118
Sse9I AATT 6 cut(s) 38, 137, 203, 304, 542, 856
SsiI CCGC 3 cut(s) 168, 181, 692
SspI AATATT 1 cut(s) 733
SspMI CTAG 3 cut(s) 326, 336, 410
StyD4I CCNGG 1 cut(s) 482
StyI CCWWGG 1 cut(s) 325
TaaI ACNGT 3 cut(s) 151, 577, 680
TaiI ACGT 3 cut(s) 120, 132, 822
TasI AATT 6 cut(s) 38, 137, 203, 304, 542, 856
TfiI GAWTC 2 cut(s) 26, 339
Tru1I TTAA 5 cut(s) 189, 225, 303, 474, 564
Tru9I TTAA 5 cut(s) 189, 225, 303, 474, 564
TscAI CASTG 5 cut(s) 117, 177, 256, 659, 769
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 1 cut(s) 75
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 350, 509
TspGWI ACGGA 1 cut(s) 74
TspRI CASTG 5 cut(s) 117, 177, 256, 659, 769
Vha464I CTTAAG 1 cut(s) 563
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 3 cut(s) 137, 203, 542
XbaI TCTAGA 1 cut(s) 335
XmaJI CCTAGG 1 cut(s) 325
XspI CTAG 3 cut(s) 326, 336, 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.