MD11G1003100.v1.1

Lysyl-tRNA synthetase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012016
Physical Location & Seq
Reverse (-)
254384 .. 258871
4488 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1003100.v1.1.491

Sequence Viewer

Length: 1800 bp
ATGGATGCGGTGAAGCTATGGAGCCTATCCTCGCAGCCACTGAAGCACCTCTTCCGCGTTGCTTGCACCACCACCACCACCACTCTCAGACGACCATTTTCTCATTCTTCTTCGTATTCTAGAGTTGTCCTCCGCTGTTGCTCTTCCACCTCCACCACCACCACCACTCCTGCCAACGCCGCCAGAGCCGGCGGCCGCAACCGGCGCGCATCGACATCTTCCTCCACCTCCGATAAAGACGCTGTTCGAGCCATTCGTTTGAAGAAGGTAGAAGAACTGAGGAGTAAAGGTTTGGAGCCATATGCTTATGGGTGGGAAAGGACTCACTCTGCTAATCAGCTGCAAGATATATACAAGGATTTAGCGGACGGTGAAGAGGCAAAAGGCGAGAAGGATGCTGTGTCCATCGCAGGAAGAATCGTGGCTCGCAGAGCGTTTGGAAAGCTTGCATTTTTGACACTGAGGGACGATTCCGGCACAATTCAGCTTTATTGTGAAAAGGAACGGCTTGCAGACGAACAATTTGATCAGCTAAAAGCACTTGTTGATATTGGTGATATAGTTGGTGCTAGTGGATCTATCAAACGGACAGAGAAAGGGGAGCTTTCGATATATGTGAATTCTTTTGCAATTCTTACAAAATCTCTACTCCCGCTGCCAGACAAGTATCATGGTCTAACAGATGTGGACAAGCGCTACCGTCAGCGGTATGTAGATATGATTGCTAATCCTGAGGTAGCTGATGTATTCCGGAAAAGAGCAAAGATTATATCGGAGATACGGAAGGCAGTGGAGTCTGTTGGTTTCATTGAGGTTGAAACTCCAGTTCTGCAGGGAGCAGCTGGTGGAGCGGAAGCTAGGCCATTTGTAACACACCATAATTCACTTGGAAGGGACCTGTATTTGAGAATAGCAACCGAGCTCCACTTAAAGAGAATGCTGGTTGGTGGGTTTGAAAAAGTGTATGAGATTGGTCGAATATTCAGGAATGAAGGCATTTCAACTCGTCATAATCCTGAATTTACCACAATAGAGATGTATGAGGCATATTCAGACTATGAAAGCATGATGAACATGGCAGAGGAAATTGTTACACAATGTGCGCTTGCAGTTCATGGGACTCTCACTGTTGATTATCAGGGGGCGGAGATACATCTTGAGCGGCCTTGGAGGAGGGAAACCATGCACAATCTTGTAAAAGAATCCACTGGGATTGATTTTGGTGAGTTGAGCAATGATCTTAAAGTGGCTAAAGAAGTTACTTTGAAAACCCTCAGAGTTGACCTTGATCACAAAGACAAAGTTTCTATTGAAGCATGCACATCTACTGGCCACCTTCTTAATGAGGTTTTTGAGTTTGCTGTAGAACCAAAGCTTCTGCAACCCACATTTGTTTTAGACTATCCTATTGAGATATCTCCTCTGGCCAAGCCACACCGAAGAAATGCAGGGTTGACTGAGAGATTTGAGCTTTTCATCTGTGGTCGTGAGCTGGCCAATGCTTTCTCTGAACTAACTGATCCTATGGATCAGAGAGGACGGTTGGAAGAGCAAGTGAGGCAGCACAATGAGAAGAAAGCAGCAGGTACTTCCGAAACAGATGGTGCAGATGATAAAGGAAAGGAAAATGATGACTTGTATGAGGTGACTGTTGATGATGATTTCCTTACAGCTTTGGAATACGGGATGCCTCCAGCTTCAGGAATGGGACTTGGAATTGACAGGCTAGTCATGCTTTTAACAAACTCGGCGAGCATTAGAGATGTAATTGCTTTCCCGGTTCTTAAAGTTCATCAATAA

Protein Analysis

600

Amino Acids

67.03

Weight (kDa)

6.04

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_anti-codon PF01336 134 - 211 1.8e-18 OB-fold nucleic acid binding domain
tRNA-synt_2 PF00152 229 - 594 3.2e-84 tRNA synthetases class II (D, K and N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012016)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13490
fragaria_vesca FvH4_3g45770 FvH4_3g45770
malus_domestica MD03G1003300.v1.1 MD11G1003100.v1.1
prunus_persica Prupe.6G002900_v2.0.a1
pyrus_communis pycom11g00280
rosa_chinensis RchiOBHm_Chr5g0083841
rosa_laevigata RLG00000037057
rosa_multiflora Rmu_sc0001287.1_g000040 Rmu_sc0024007.1_g000006
rosa_roxburghii Rroxscaffold_1G00000750
rosa_rugosa Rorug05G0486400 Rorug05G0486500
rosa_samantha Rh5AG539600 Rh5BG564700 Rh5CG586600 Rh5DG573200
rosa_wichuraiana Rw5G050170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1727
Acc36I ACCTGC 1 cut(s) 1574
AccBSI CCGCTC 2 cut(s) 851, 1162
AccII CGCG 2 cut(s) 57, 207
AccIII TCCGGA 1 cut(s) 750
AclWI GGATC 3 cut(s) 583, 1514, 1536
AcoI YGGCCR 4 cut(s) 193, 1330, 1425, 1494
AcsI RAATTY 2 cut(s) 619, 1019
AcuI CTGAAG 2 cut(s) 62, 1683
AdeI CACNNNGTG 1 cut(s) 1100
AfaI GTAC 1 cut(s) 1588
AfeI AGCGCT 1 cut(s) 695
AfiI CCNNNNNNNGG 1 cut(s) 1700
AgsI TTSAA 6 cut(s) 262, 818, 956, 1002, 1267, 1313
Alw21I GWGCWC 1 cut(s) 924
AlwI GGATC 3 cut(s) 583, 1514, 1536
AlwNI CAGNNNCTG 1 cut(s) 40
Aor13HI TCCGGA 1 cut(s) 750
Aor51HI AGCGCT 1 cut(s) 695
AoxI GGCC 6 cut(s) 193, 860, 1163, 1330, 1425, 1494
ApeKI GCWGC 6 cut(s) 34, 340, 655, 839, 1561, 1580
ApoI RAATTY 2 cut(s) 619, 1019
ArsI GACNNNNNNTTYG 2 cut(s) 506, 538
AspLEI GCGC 4 cut(s) 207, 209, 696, 1105
AspS9I GGNCC 1 cut(s) 895
AsuC2I CCSGG 1 cut(s) 1778
AsuHPI GGTGA 5 cut(s) 22, 383, 566, 1235, 1657
AvaII GGWCC 1 cut(s) 895
AxyI CCTNAGG 1 cut(s) 732
BalI TGGCCA 3 cut(s) 1332, 1427, 1496
BanII GRGCYC 1 cut(s) 924
Bbv12I GWGCWC 1 cut(s) 924
BbvI GCAGC 6 cut(s) 46, 327, 642, 851, 1573, 1592
BccI CCATC 2 cut(s) 413, 1595
BceAI ACGGC 1 cut(s) 521
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BclI TGATCA 2 cut(s) 526, 1288
BcnI CCSGG 1 cut(s) 1778
BfaI CTAG 4 cut(s) 120, 570, 858, 1727
BfmI CTRYAG 2 cut(s) 830, 1362
BfoI RGCGCY 1 cut(s) 697
BfuAI ACCTGC 1 cut(s) 1574
Bme1390I CCNGG 1 cut(s) 1778
Bme18I GGWCC 1 cut(s) 895
BmgT120I GGNCC 1 cut(s) 895
BmiI GGNNCC 3 cut(s) 23, 297, 896
BmrFI CCNGG 1 cut(s) 1778
BmrI ACTGGG 1 cut(s) 1218
BmsI GCATC 3 cut(s) 218, 385, 1677
BmuI ACTGGG 1 cut(s) 1218
BplI GAGNNNNNCTC 2 cut(s) 114, 146
BpmI CTGGAG 2 cut(s) 807, 1677
BpuEI CTTGAG 1 cut(s) 1178
BpuMI CCSGG 1 cut(s) 1778
BsaJI CCNNGG 1 cut(s) 1166
BsaWI WCCGGW 1 cut(s) 750
BsaXI ACNNNNNCTCC 4 cut(s) 151, 181, 274, 304
Bsc4I CCNNNNNNNGG 1 cut(s) 1700
Bse118I RCCGGY 2 cut(s) 188, 201
Bse1I ACTGG 3 cut(s) 824, 1213, 1333
Bse21I CCTNAGG 1 cut(s) 732
Bse3DI GCAATG 1 cut(s) 1240
BseAI TCCGGA 1 cut(s) 750
BseDI CCNNGG 1 cut(s) 1166
BseGI GGATG 3 cut(s) 10, 400, 1692
BseLI CCNNNNNNNGG 1 cut(s) 1700
BseMI GCAATG 1 cut(s) 1240
BseMII CTCAG 6 cut(s) 100, 269, 452, 723, 1288, 1449
BseNI ACTGG 3 cut(s) 824, 1213, 1333
BsePI GCGCGC 1 cut(s) 205
BseRI GAGGAG 3 cut(s) 295, 1186, 1410
BseX3I CGGCCG 1 cut(s) 193
BseXI GCAGC 6 cut(s) 46, 327, 642, 851, 1573, 1592
BsgI GTGCAG 1 cut(s) 1626
Bsh1236I CGCG 2 cut(s) 57, 207
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 6 cut(s) 195, 862, 1165, 1332, 1427, 1496
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 924
BsiSI CCGG 5 cut(s) 189, 202, 474, 751, 1778
BslFI GGGAC 4 cut(s) 479, 908, 1132, 1722
BslI CCNNNNNNNGG 1 cut(s) 1700
BsmFI GGGAC 4 cut(s) 479, 908, 1132, 1722
BsmI GAATGC 1 cut(s) 942
BsnI GGCC 6 cut(s) 195, 862, 1165, 1332, 1427, 1496
Bsp1286I GDGCHC 1 cut(s) 924
Bsp13I TCCGGA 1 cut(s) 750
Bsp143I GATC 6 cut(s) 526, 575, 1237, 1288, 1519, 1528
BspANI GGCC 6 cut(s) 195, 862, 1165, 1332, 1427, 1496
BspCNI CTCAG 6 cut(s) 99, 270, 453, 724, 1287, 1450
BspEI TCCGGA 1 cut(s) 750
BspFNI CGCG 2 cut(s) 57, 207
BspLI GGNNCC 3 cut(s) 23, 297, 896
BspMAI CTGCAG 1 cut(s) 834
BspMI ACCTGC 1 cut(s) 1574
BspPI GGATC 3 cut(s) 583, 1514, 1536
BspQI GCTCTTC 2 cut(s) 148, 1542
BsrBI CCGCTC 2 cut(s) 851, 1162
BsrDI GCAATG 1 cut(s) 1240
BsrFI RCCGGY 2 cut(s) 188, 201
BsrI ACTGG 3 cut(s) 824, 1213, 1333
BssAI RCCGGY 2 cut(s) 188, 201
BssECI CCNNGG 1 cut(s) 1166
BssHII GCGCGC 1 cut(s) 205
BssMI GATC 6 cut(s) 526, 575, 1237, 1288, 1519, 1528
BssT1I CCWWGG 1 cut(s) 1166
Bst4CI ACNGT 5 cut(s) 371, 701, 1129, 1542, 1651
Bst6I CTCTTC 4 cut(s) 56, 148, 369, 1542
BstDEI CTNAG 6 cut(s) 86, 278, 461, 732, 1274, 1458
BstF5I GGATG 3 cut(s) 10, 400, 1692
BstFNI CGCG 2 cut(s) 57, 207
BstH2I RGCGCY 1 cut(s) 697
BstHHI GCGC 4 cut(s) 207, 209, 696, 1105
BstKTI GATC 6 cut(s) 529, 578, 1240, 1291, 1522, 1531
BstMBI GATC 6 cut(s) 526, 575, 1237, 1288, 1519, 1528
BstMCI CGRYCG 1 cut(s) 196
BstNSI RCATGY 1 cut(s) 1320
BstSCI CCNGG 1 cut(s) 1776
BstSFI CTRYAG 2 cut(s) 830, 1362
BstUI CGCG 2 cut(s) 57, 207
BstV1I GCAGC 6 cut(s) 46, 327, 642, 851, 1573, 1592
BstX2I RGATCY 1 cut(s) 575
BstYI RGATCY 1 cut(s) 575
BstZI CGGCCG 1 cut(s) 193
Bsu36I CCTNAGG 1 cut(s) 732
BsuRI GGCC 6 cut(s) 195, 862, 1165, 1332, 1427, 1496
BtgZI GCGATG 1 cut(s) 391
BtsCI GGATG 3 cut(s) 10, 400, 1692
BtsI GCAGTG 1 cut(s) 795
BtsIMutI CAGTG 5 cut(s) 38, 458, 795, 1125, 1206
BveI ACCTGC 1 cut(s) 1574
CaiI CAGNNNCTG 1 cut(s) 40
CciNI GCGGCCGC 1 cut(s) 193
CfoI GCGC 4 cut(s) 207, 209, 696, 1105
Cfr10I RCCGGY 2 cut(s) 188, 201
Cfr13I GGNCC 1 cut(s) 895
CseI GACGC 1 cut(s) 248
Csp6I GTAC 1 cut(s) 1587
CspCI CAANNNNNGTGG 2 cut(s) 1195, 1230
CviAII CATG 7 cut(s) 671, 1066, 1075, 1115, 1183, 1317, 1732
CviQI GTAC 1 cut(s) 1587
DdeI CTNAG 6 cut(s) 86, 278, 461, 732, 1274, 1458
DpnI GATC 6 cut(s) 528, 577, 1239, 1290, 1521, 1530
DpnII GATC 6 cut(s) 526, 575, 1237, 1288, 1519, 1528
DraIII CACNNNGTG 1 cut(s) 1100
DrdI GACNNNNNNGTC 1 cut(s) 1727
DseDI GACNNNNNNGTC 1 cut(s) 1727
EaeI YGGCCR 4 cut(s) 193, 1330, 1425, 1494
EagI CGGCCG 1 cut(s) 193
Eam1104I CTCTTC 4 cut(s) 56, 148, 369, 1542
EarI CTCTTC 4 cut(s) 56, 148, 369, 1542
EciI GGCGGA 1 cut(s) 1160
Ecl136II GAGCTC 1 cut(s) 922
EclXI CGGCCG 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 1166
Eco24I GRGCYC 1 cut(s) 924
Eco32I GATATC 1 cut(s) 1416
Eco47I GGWCC 1 cut(s) 895
Eco47III AGCGCT 1 cut(s) 695
Eco52I CGGCCG 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 922
Eco57I CTGAAG 2 cut(s) 62, 1683
Eco81I CCTNAGG 1 cut(s) 732
EcoICRI GAGCTC 1 cut(s) 922
EcoO109I RGGNCCY 1 cut(s) 895
EcoRI GAATTC 1 cut(s) 619
EcoRV GATATC 1 cut(s) 1416
EcoT14I CCWWGG 1 cut(s) 1166
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 1 cut(s) 1166
FaeI CATG 7 cut(s) 674, 1069, 1078, 1118, 1186, 1320, 1735
FalI AAGNNNNNCTT 4 cut(s) 35, 67, 588, 620
FaqI GGGAC 4 cut(s) 479, 908, 1132, 1722
FatI CATG 7 cut(s) 670, 1065, 1074, 1114, 1182, 1316, 1731
FauI CCCGC 1 cut(s) 660
FauNDI CATATG 1 cut(s) 301
FbaI TGATCA 2 cut(s) 526, 1288
FokI GGATG 3 cut(s) 17, 407, 1699
FriOI GRGCYC 1 cut(s) 924
FspBI CTAG 4 cut(s) 120, 570, 858, 1727
GlaI GCGC 4 cut(s) 206, 208, 695, 1104
GsuI CTGGAG 2 cut(s) 807, 1677
HaeII RGCGCY 1 cut(s) 697
HaeIII GGCC 6 cut(s) 195, 862, 1165, 1332, 1427, 1496
HapII CCGG 5 cut(s) 189, 202, 474, 751, 1778
HgaI GACGC 1 cut(s) 248
HhaI GCGC 4 cut(s) 207, 209, 696, 1105
Hin1II CATG 7 cut(s) 674, 1069, 1078, 1118, 1186, 1320, 1735
Hin6I GCGC 4 cut(s) 205, 207, 694, 1103
HinP1I GCGC 4 cut(s) 205, 207, 694, 1103
HincII GTYRAC 2 cut(s) 1282, 1455
HindII GTYRAC 2 cut(s) 1282, 1455
HindIII AAGCTT 2 cut(s) 443, 1373
HinfI GANTC 6 cut(s) 322, 417, 470, 794, 1120, 1202
HpaII CCGG 5 cut(s) 189, 202, 474, 751, 1778
HphI GGTGA 5 cut(s) 22, 383, 566, 1235, 1657
Hpy166II GTNNAC 3 cut(s) 688, 1282, 1455
Hpy188I TCNGA 8 cut(s) 89, 232, 775, 1054, 1277, 1510, 1533, 1594
Hpy188III TCNNGA 8 cut(s) 120, 731, 751, 985, 1016, 1157, 1487, 1701
Hpy8I GTNNAC 3 cut(s) 688, 1282, 1455
HpyAV CCTTC 6 cut(s) 259, 385, 778, 885, 986, 1346
HpyCH4III ACNGT 5 cut(s) 371, 701, 1129, 1542, 1651
HpyF3I CTNAG 6 cut(s) 86, 278, 461, 732, 1274, 1458
Hsp92II CATG 7 cut(s) 674, 1069, 1078, 1118, 1186, 1320, 1735
HspAI GCGC 4 cut(s) 205, 207, 694, 1103
Kpn2I TCCGGA 1 cut(s) 750
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 2 cut(s) 526, 1288
Kzo9I GATC 6 cut(s) 526, 575, 1237, 1288, 1519, 1528
LguI GCTCTTC 2 cut(s) 148, 1542
LmnI GCTCC 6 cut(s) 21, 295, 601, 836, 848, 927
Lsp1109I GCAGC 6 cut(s) 46, 327, 642, 851, 1573, 1592
LweI GCATC 3 cut(s) 218, 385, 1677
MaeI CTAG 4 cut(s) 120, 570, 858, 1727
MaeIII GTNAC 4 cut(s) 868, 1090, 1258, 1645
MalI GATC 6 cut(s) 528, 577, 1239, 1290, 1521, 1530
MbiI CCGCTC 2 cut(s) 851, 1162
MboI GATC 6 cut(s) 526, 575, 1237, 1288, 1519, 1528
MflI RGATCY 1 cut(s) 575
MhlI GDGCHC 1 cut(s) 924
MlsI TGGCCA 3 cut(s) 1332, 1427, 1496
MluCI AATT 9 cut(s) 480, 521, 619, 630, 880, 1019, 1086, 1716, 1767
MluNI TGGCCA 3 cut(s) 1332, 1427, 1496
MlyI GAGTC 3 cut(s) 316, 803, 1114
MmeI TCCRAC 1 cut(s) 1524
Mox20I TGGCCA 3 cut(s) 1332, 1427, 1496
MroI TCCGGA 1 cut(s) 750
MroNI GCCGGC 1 cut(s) 188
MscI TGGCCA 3 cut(s) 1332, 1427, 1496
MseI TTAA 5 cut(s) 929, 1242, 1341, 1739, 1785
Msp20I TGGCCA 3 cut(s) 1332, 1427, 1496
MspA1I CMGCKG 5 cut(s) 135, 340, 655, 706, 842
MspI CCGG 5 cut(s) 189, 202, 474, 751, 1778
MspR9I CCNGG 1 cut(s) 1778
Mva1269I GAATGC 1 cut(s) 942
MvnI CGCG 2 cut(s) 57, 207
NaeI GCCGGC 1 cut(s) 190
NciI CCSGG 1 cut(s) 1778
NdeI CATATG 1 cut(s) 301
NdeII GATC 6 cut(s) 526, 575, 1237, 1288, 1519, 1528
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 7 cut(s) 674, 1069, 1078, 1118, 1186, 1320, 1735
NlaIV GGNNCC 3 cut(s) 23, 297, 896
NmeAIII GCCGAG 1 cut(s) 1727
NmuCI GTSAC 1 cut(s) 1645
NotI GCGGCCGC 1 cut(s) 193
NspI RCATGY 1 cut(s) 1320
PaeI GCATGC 1 cut(s) 1320
PauI GCGCGC 1 cut(s) 205
PciSI GCTCTTC 2 cut(s) 148, 1542
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PctI GAATGC 1 cut(s) 942
PdiI GCCGGC 1 cut(s) 190
PfeI GAWTC 3 cut(s) 417, 470, 1202
PleI GAGTC 3 cut(s) 316, 802, 1114
PpsI GAGTC 3 cut(s) 316, 802, 1114
PpuMI RGGWCCY 1 cut(s) 895
Psp124BI GAGCTC 1 cut(s) 924
Psp5II RGGWCCY 1 cut(s) 895
PspN4I GGNNCC 3 cut(s) 23, 297, 896
PspPI GGNCC 1 cut(s) 895
PspPPI RGGWCCY 1 cut(s) 895
PstI CTGCAG 1 cut(s) 834
PstNI CAGNNNCTG 1 cut(s) 40
PsuI RGATCY 1 cut(s) 575
PteI GCGCGC 1 cut(s) 205
PvuII CAGCTG 2 cut(s) 340, 842
RsaI GTAC 1 cut(s) 1588
RsaNI GTAC 1 cut(s) 1587
SacI GAGCTC 1 cut(s) 924
SapI GCTCTTC 2 cut(s) 148, 1542
SaqAI TTAA 5 cut(s) 929, 1242, 1341, 1739, 1785
Sau3AI GATC 6 cut(s) 526, 575, 1237, 1288, 1519, 1528
Sau96I GGNCC 1 cut(s) 895
SchI GAGTC 3 cut(s) 316, 803, 1114
ScrFI CCNGG 1 cut(s) 1778
SduI GDGCHC 1 cut(s) 924
SfaNI GCATC 3 cut(s) 218, 385, 1677
SfcI CTRYAG 2 cut(s) 830, 1362
SinI GGWCC 1 cut(s) 895
SmlI CTYRAG 1 cut(s) 1157
SmoI CTYRAG 1 cut(s) 1157
SphI GCATGC 1 cut(s) 1320
Sse9I AATT 9 cut(s) 480, 521, 619, 630, 880, 1019, 1086, 1716, 1767
SspI AATATT 1 cut(s) 981
SspMI CTAG 4 cut(s) 120, 570, 858, 1727
SstI GAGCTC 1 cut(s) 924
StyD4I CCNGG 1 cut(s) 1776
StyI CCWWGG 1 cut(s) 1166
TaaI ACNGT 5 cut(s) 371, 701, 1129, 1542, 1651
TaqI TCGA 4 cut(s) 212, 247, 608, 976
TasI AATT 9 cut(s) 480, 521, 619, 630, 880, 1019, 1086, 1716, 1767
TauI GCSGC 4 cut(s) 182, 195, 198, 1165
TfiI GAWTC 3 cut(s) 417, 470, 1202
Tru1I TTAA 5 cut(s) 929, 1242, 1341, 1739, 1785
Tru9I TTAA 5 cut(s) 929, 1242, 1341, 1739, 1785
TscAI CASTG 5 cut(s) 45, 465, 795, 1132, 1213
TseFI GTSAC 1 cut(s) 1645
TseI GCWGC 6 cut(s) 34, 340, 655, 839, 1561, 1580
Tsp45I GTSAC 1 cut(s) 1645
TspDTI ATGAA 7 cut(s) 796, 1005, 1074, 1085, 1103, 1465, 1781
TspGWI ACGGA 2 cut(s) 601, 796
TspRI CASTG 5 cut(s) 45, 465, 795, 1132, 1213
VpaK11BI GGWCC 1 cut(s) 895
XapI RAATTY 2 cut(s) 619, 1019
XbaI TCTAGA 1 cut(s) 119
XceI RCATGY 1 cut(s) 1320
XcmI CCANNNNNNNNNTGG 1 cut(s) 884
XspI CTAG 4 cut(s) 120, 570, 858, 1727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.