Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0015027

Physical Location & Seq

Genomic Coordinates Forward (+)

4364593 .. 4365969

Gene Structure 1377 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1054700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGATGCCGGGGAGCGAAGAACGACAGCCGCTGCTGGCGCCTGCGCCGCCGCAGCAGCAGCGTATCCCCGACAATGCCCTGTTCGGGAAGTACGAGCTCGGCAAGCTCCTCGGGTGCGGCGCCTTCGCCAAAGTCTACCACGCCCGCAACGTCCTCACTGGCCAGAGTGTCGCCGTGAAAATCATCAACAAGAAGAAGCTCACCGGCACCAACCTCATGTCCAACATCAAGCGCGAGATCACCATCATGCGCCGCCTCCGCCACCCCAACATCGTCAAGCTCTACGAGGTCCTCGCCTCCAAGACCAAGATCTACTTCGTCGTGGAGTTCGTCAAGGGTGGAGAGCTTTTCGCCAAGGTGTCGAAGGGGCGGTTCTCAGAGAATCTCAGCCGGAAGTACTTCCAGCAGCTCATCTCCGCCGTCGGGTACTGCCACTCCCGCGGGATCTACCACCGCGATTTGAAACCCGAGAATTTGTTAATTGACGAGAGCGGGAATTTGAAGGTTTCGGATTTCGGGCTCAGCGCCGTGGCCGACCAGATCCGACCCGACGGGCTCCTCCACACGCTTTGCGGGACCCCTGCTTACGTGGCGCCGGAGATTTTGACGAAGAAGGGATACGACGGCGCGAAGGTGGATGTGTGGTCGTGCGGCGTGATACTGTACGTTTTGAACGCCGGGTATTTGCCGTTCAACGACCCCAATTTGATGGCGATGTATAAGAAGATTTACAAGGGCGAGTTCCGGTGCCCGAAATGGATGTCGCCGGATCTGAAGCGGTTTTTGGGTCGGCTCATGGATACCAACCCGGCGACCCGGATTACGATCGACGAGATTTTGAAGGACCCGTGGTTCAGAAAAGGCGGGTGCAAGGAAATCAAATTCTACGACGACGATTGTTCGATGGACGACGCCAAAATCGGCGGCGGCGAGGAGGAACAGAAGGGCGTGACGAGCCTGAACGCGTTCGATTTGATTTCGTTCTCGGCCGGGTTGGACCTGTCCGGGTTGTTCGAGTCGTCGAGCGGCAGCCCTGCGGAGGACGGCGAGCGCTTTATATCGGAGGAGACGGTGGAGAAGGTGATGGAGAGAGTGGCTGAGTTTGCCAAGGCGGAGAAGTTGAGGGTGAGGAGGAAGAAGGATTGGGGACTGGAGATGGAAGGGCAGAATGGAAATTTATCAATCGCTGTGGAGGTTTACAGATTGACTGATAATCTGGTGGTGGTGGAGGCCAAGAGGACGGGCGGAGACGCCGCGCCGTTCAAGGAGATGTGGAAGAACAAGCTCAGGCCTTATCTGATTTGCCCAAATGATCAAAGCATCTCATCGTCCATGTCTTCTTCCTCCTCGCTCGCTTGCCCAGTCAACGAATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009268 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04113 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04113 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

459

Amino Acids

51.16

Weight (kDa)

8.79

Isoelectric Point (pI)

45.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	31 - 285	3.7e-75	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	32 - 281	6.1e-39	Protein tyrosine and serine/threonine kinase
NAF	PF03822	318 - 377	1.2e-19	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015027)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30360
fragaria_vesca	FvH4_3g40260
malus_domestica	MD03G1054700.v1.1 MD11G1056100.v1.1
prunus_persica	Prupe.6G043300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0072521
rosa_laevigata	RLG00000036309
rosa_multiflora	Rmu_co8287111.1_g000001 Rmu_co8453861.1_g000001 Rmu_sc0007873.1_g000002
rosa_roxburghii	Rroxscaffold_1G00008620
rosa_rugosa	Rorug05G0418900
rosa_samantha	Rh5AG475200 Rh5BG495500 Rh5CG519300
rosa_wichuraiana	Rw5G044150

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	5 cut(s)	37, 119, 206, 592, 747
AccBSI	CCGCTC	2 cut(s)	492, 1026
AccI	GTMKAC	1 cut(s)	135
AccII	CGCG	6 cut(s)	234, 441, 456, 629, 965, 1256
AclWI	GGATC	3 cut(s)	452, 535, 777
AcoI	YGGCCR	3 cut(s)	160, 531, 987
AcsI	RAATTY	4 cut(s)	472, 496, 881, 1174
AcuI	CTGAAG	1 cut(s)	794
AcyI	GRCGYC	5 cut(s)	38, 120, 593, 912, 1251
AfaI	GTAC	4 cut(s)	92, 398, 428, 665
AfeI	AGCGCT	1 cut(s)	1052
AfiI	CCNNNNNNNGG	3 cut(s)	84, 423, 1039
AflIII	ACRYGT	1 cut(s)	963
AgsI	TTSAA	6 cut(s)	463, 502, 673, 694, 841, 1264
AjuI	GAANNNNNNNTTGG	4 cut(s)	299, 331, 767, 799
AloI	GAACNNNNNNTCC	2 cut(s)	836, 868
AluBI	AGCT	7 cut(s)	97, 106, 199, 280, 346, 409, 1285
AluI	AGCT	7 cut(s)	97, 106, 199, 280, 346, 409, 1285
Alw21I	GWGCWC	1 cut(s)	99
Alw26I	GTCTC	2 cut(s)	1061, 1242
AlwI	GGATC	3 cut(s)	452, 535, 777
AlwNI	CAGNNNCTG	1 cut(s)	31
Ama87I	CYCGRG	2 cut(s)	110, 467
Aor51HI	AGCGCT	1 cut(s)	1052
AoxI	GGCC	5 cut(s)	160, 531, 987, 1230, 1289
ApeKI	GCWGC	6 cut(s)	31, 52, 55, 58, 406, 1029
ApoI	RAATTY	4 cut(s)	472, 496, 881, 1174
ArsI	GACNNNNNNTTYG	2 cut(s)	746, 778
Asp700I	GAANNNNTTC	2 cut(s)	398, 965
AspS9I	GGNCC	4 cut(s)	289, 576, 844, 997
AsuC2I	CCSGG	6 cut(s)	9, 679, 809, 817, 991, 1006
AsuHPI	GGTGA	4 cut(s)	193, 232, 1093, 1138
AvaI	CYCGRG	2 cut(s)	110, 467
AvaII	GGWCC	4 cut(s)	289, 576, 844, 997
BaeGI	GKGCMC	1 cut(s)	752
BalI	TGGCCA	1 cut(s)	162
BanI	GGYRCC	5 cut(s)	37, 119, 206, 592, 747
BanII	GRGCYC	3 cut(s)	99, 522, 558
BarI	GAAGNNNNNNTAC	2 cut(s)	602, 634
BbsI	GAAGAC	1 cut(s)	1329
Bbv12I	GWGCWC	1 cut(s)	99
BbvI	GCAGC	6 cut(s)	18, 64, 67, 70, 418, 1041
BccI	CCATC	5 cut(s)	251, 703, 898, 1078, 1150
BceAI	ACGGC	7 cut(s)	158, 404, 512, 640, 673, 1060, 1243
BcgI	CGANNNNNNTGC	2 cut(s)	91, 125
BciVI	GTATCC	3 cut(s)	74, 611, 793
BclI	TGATCA	1 cut(s)	1312
BcnI	CCSGG	6 cut(s)	9, 679, 809, 817, 991, 1006
BcoDI	GTCTC	2 cut(s)	1061, 1242
BfoI	RGCGCY	5 cut(s)	41, 123, 528, 596, 1054
BfuI	GTATCC	3 cut(s)	74, 611, 793
BglII	AGATCT	1 cut(s)	309
BlpI	GCTNAGC	1 cut(s)	521
BmcAI	AGTACT	1 cut(s)	398
Bme1390I	CCNGG	6 cut(s)	9, 679, 809, 817, 991, 1006
Bme18I	GGWCC	4 cut(s)	289, 576, 844, 997
BmeT110I	CYCGRG	2 cut(s)	110, 467
BmgT120I	GGNCC	4 cut(s)	289, 576, 844, 997
BmiI	GGNNCC	9 cut(s)	39, 121, 208, 557, 577, 578, 594, 749, 846
BmrFI	CCNGG	6 cut(s)	9, 679, 809, 817, 991, 1006
BmrI	ACTGGG	1 cut(s)	1355
BmsI	GCATC	1 cut(s)	1329
BmuI	ACTGGG	1 cut(s)	1355
BpiI	GAAGAC	1 cut(s)	1329
BpmI	CTGGAG	1 cut(s)	1172
Bpu10I	CCTNAGC	1 cut(s)	1286
Bpu1102I	GCTNAGC	1 cut(s)	521
BpuMI	CCSGG	6 cut(s)	9, 679, 809, 817, 991, 1006
BsaAI	YACGTR	1 cut(s)	589
BsaBI	GATNNNNATC	2 cut(s)	242, 824
BsaHI	GRCGYC	5 cut(s)	38, 120, 593, 912, 1251
BsaJI	CCNNGG	7 cut(s)	8, 109, 354, 439, 528, 848, 1107
BsaWI	WCCGGW	1 cut(s)	744
BsaXI	ACNNNNNCTCC	2 cut(s)	419, 449
Bsc4I	CCNNNNNNNGG	3 cut(s)	84, 423, 1039
Bse118I	RCCGGY	1 cut(s)	203
Bse1I	ACTGG	3 cut(s)	163, 1155, 1361
Bse8I	GATNNNNATC	2 cut(s)	242, 824
BseDI	CCNNGG	7 cut(s)	8, 109, 354, 439, 528, 848, 1107
BseGI	GGATG	2 cut(s)	643, 765
BseJI	GATNNNNATC	2 cut(s)	242, 824
BseLI	CCNNNNNNNGG	3 cut(s)	84, 423, 1039
BseMII	CTCAG	5 cut(s)	390, 400, 535, 1089, 1300
BseNI	ACTGG	3 cut(s)	163, 1155, 1361
BseRI	GAGGAG	6 cut(s)	98, 548, 947, 1079, 1144, 1336
BseSI	GKGCMC	1 cut(s)	752
BseX3I	CGGCCG	1 cut(s)	987
BseXI	GCAGC	6 cut(s)	18, 64, 67, 70, 418, 1041
Bsh1236I	CGCG	6 cut(s)	234, 441, 456, 629, 965, 1256
Bsh1285I	CGRYCG	2 cut(s)	828, 990
BshFI	GGCC	5 cut(s)	162, 533, 989, 1232, 1291
BshNI	GGYRCC	5 cut(s)	37, 119, 206, 592, 747
BsiEI	CGRYCG	2 cut(s)	828, 990
BsiHKAI	GWGCWC	1 cut(s)	99
BsiHKCI	CYCGRG	2 cut(s)	110, 467
BslFI	GGGAC	2 cut(s)	589, 1161
BslI	CCNNNNNNNGG	3 cut(s)	84, 423, 1039
BsmAI	GTCTC	2 cut(s)	1061, 1242
BsmBI	CGTCTC	2 cut(s)	1061, 1242
BsmFI	GGGAC	2 cut(s)	589, 1161
BsmI	GAATGC	1 cut(s)	1376
BsnI	GGCC	5 cut(s)	162, 533, 989, 1232, 1291
BsoBI	CYCGRG	2 cut(s)	110, 467
Bsp1286I	GDGCHC	4 cut(s)	99, 522, 558, 752
Bsp143I	GATC	7 cut(s)	237, 309, 444, 540, 769, 825, 1312
Bsp1720I	GCTNAGC	1 cut(s)	521
BspANI	GGCC	5 cut(s)	162, 533, 989, 1232, 1291
BspCNI	CTCAG	5 cut(s)	389, 399, 534, 1090, 1299
BspFNI	CGCG	6 cut(s)	234, 441, 456, 629, 965, 1256
BspLI	GGNNCC	9 cut(s)	39, 121, 208, 557, 577, 578, 594, 749, 846
BspPI	GGATC	3 cut(s)	452, 535, 777
BspT107I	GGYRCC	5 cut(s)	37, 119, 206, 592, 747
BsrBI	CCGCTC	2 cut(s)	492, 1026
BsrFI	RCCGGY	1 cut(s)	203
BsrI	ACTGG	3 cut(s)	163, 1155, 1361
BssAI	RCCGGY	1 cut(s)	203
BssECI	CCNNGG	7 cut(s)	8, 109, 354, 439, 528, 848, 1107
BssMI	GATC	7 cut(s)	237, 309, 444, 540, 769, 825, 1312
BssNI	GRCGYC	5 cut(s)	38, 120, 593, 912, 1251
BssT1I	CCWWGG	2 cut(s)	354, 1107
Bst4CI	ACNGT	2 cut(s)	663, 1072
BstACI	GRCGYC	5 cut(s)	38, 120, 593, 912, 1251
BstBAI	YACGTR	1 cut(s)	589
BstC8I	GCNNGC	7 cut(s)	36, 42, 104, 145, 1049, 1353, 1357
BstDEI	CTNAG	5 cut(s)	376, 386, 521, 1098, 1286
BstDSI	CCRYGG	3 cut(s)	439, 528, 848
BstF5I	GGATG	2 cut(s)	643, 765
BstFNI	CGCG	6 cut(s)	234, 441, 456, 629, 965, 1256
BstH2I	RGCGCY	5 cut(s)	41, 123, 528, 596, 1054
BstKTI	GATC	7 cut(s)	240, 312, 447, 543, 772, 828, 1315
BstMAI	GTCTC	2 cut(s)	1061, 1242
BstMBI	GATC	7 cut(s)	237, 309, 444, 540, 769, 825, 1312
BstMCI	CGRYCG	2 cut(s)	828, 990
BstSCI	CCNGG	6 cut(s)	7, 677, 807, 815, 989, 1004
BstSLI	GKGCMC	1 cut(s)	752
BstUI	CGCG	6 cut(s)	234, 441, 456, 629, 965, 1256
BstV1I	GCAGC	6 cut(s)	18, 64, 67, 70, 418, 1041
BstV2I	GAAGAC	1 cut(s)	1329
BstX2I	RGATCY	4 cut(s)	309, 444, 540, 769
BstXI	CCANNNNNNTGG	1 cut(s)	709
BstYI	RGATCY	4 cut(s)	309, 444, 540, 769
BstZI	CGGCCG	1 cut(s)	987
BsuI	GTATCC	3 cut(s)	74, 611, 793
BsuRI	GGCC	5 cut(s)	162, 533, 989, 1232, 1291
BtgI	CCRYGG	3 cut(s)	439, 528, 848
BtgZI	GCGATG	1 cut(s)	728
BtsCI	GGATG	2 cut(s)	643, 765
BtsIMutI	CAGTG	1 cut(s)	156
Cac8I	GCNNGC	7 cut(s)	36, 42, 104, 145, 1049, 1353, 1357
CaiI	CAGNNNCTG	1 cut(s)	31
Cfr10I	RCCGGY	1 cut(s)	203
Cfr13I	GGNCC	4 cut(s)	289, 576, 844, 997
Cfr42I	CCGCGG	1 cut(s)	442
CseI	GACGC	2 cut(s)	920, 1259
Csp6I	GTAC	4 cut(s)	91, 397, 427, 664
CspCI	CAANNNNNGTGG	4 cut(s)	551, 586, 1170, 1205
CviAII	CATG	4 cut(s)	217, 247, 796, 1333
CviQI	GTAC	4 cut(s)	91, 397, 427, 664
DdeI	CTNAG	5 cut(s)	376, 386, 521, 1098, 1286
DinI	GGCGCC	3 cut(s)	39, 121, 594
DpnI	GATC	7 cut(s)	239, 311, 446, 542, 771, 827, 1314
DpnII	GATC	7 cut(s)	237, 309, 444, 540, 769, 825, 1312
EaeI	YGGCCR	3 cut(s)	160, 531, 987
EagI	CGGCCG	1 cut(s)	987
EciI	GGCGGA	4 cut(s)	248, 406, 1127, 1260
Ecl136II	GAGCTC	1 cut(s)	97
EclXI	CGGCCG	1 cut(s)	987
Eco130I	CCWWGG	2 cut(s)	354, 1107
Eco147I	AGGCCT	1 cut(s)	1291
Eco24I	GRGCYC	3 cut(s)	99, 522, 558
Eco47I	GGWCC	4 cut(s)	289, 576, 844, 997
Eco47III	AGCGCT	1 cut(s)	1052
Eco52I	CGGCCG	1 cut(s)	987
Eco53kI	GAGCTC	1 cut(s)	97
Eco57I	CTGAAG	1 cut(s)	794
Eco88I	CYCGRG	2 cut(s)	110, 467
EcoICRI	GAGCTC	1 cut(s)	97
EcoO109I	RGGNCCY	3 cut(s)	289, 576, 844
EcoT14I	CCWWGG	2 cut(s)	354, 1107
EcoT38I	GRGCYC	3 cut(s)	99, 522, 558
EgeI	GGCGCC	3 cut(s)	39, 121, 594
EheI	GGCGCC	3 cut(s)	39, 121, 594
ErhI	CCWWGG	2 cut(s)	354, 1107
Esp3I	CGTCTC	2 cut(s)	1061, 1242
FaeI	CATG	4 cut(s)	220, 250, 799, 1336
FaiI	YATR	6 cut(s)	218, 248, 720, 797, 1058, 1334
FalI	AAGNNNNNCTT	2 cut(s)	299, 331
FaqI	GGGAC	2 cut(s)	589, 1161
FatI	CATG	4 cut(s)	216, 246, 795, 1332
FauI	CCCGC	6 cut(s)	152, 434, 446, 485, 566, 857
FbaI	TGATCA	1 cut(s)	1312
FblI	GTMKAC	1 cut(s)	135
FokI	GGATG	2 cut(s)	650, 772
FriOI	GRGCYC	3 cut(s)	99, 522, 558
GsuI	CTGGAG	1 cut(s)	1172
HaeII	RGCGCY	5 cut(s)	41, 123, 528, 596, 1054
HaeIII	GGCC	5 cut(s)	162, 533, 989, 1232, 1291
HgaI	GACGC	2 cut(s)	920, 1259
Hin1I	GRCGYC	5 cut(s)	38, 120, 593, 912, 1251
Hin1II	CATG	4 cut(s)	220, 250, 799, 1336
HincII	GTYRAC	1 cut(s)	1366
HindII	GTYRAC	1 cut(s)	1366
HinfI	GANTC	2 cut(s)	382, 1016
HphI	GGTGA	4 cut(s)	193, 232, 1093, 1138
Hpy166II	GTNNAC	3 cut(s)	136, 1198, 1366
Hpy188I	TCNGA	7 cut(s)	379, 511, 545, 774, 857, 1063, 1299
Hpy188III	TCNNGA	1 cut(s)	85
Hpy8I	GTNNAC	3 cut(s)	136, 1198, 1366
Hpy99I	CGWCG	9 cut(s)	323, 425, 554, 626, 833, 893, 896, 914, 1024
HpyCH4III	ACNGT	2 cut(s)	663, 1072
HpyCH4IV	ACGT	3 cut(s)	150, 588, 666
HpyCH4V	TGCA	1 cut(s)	870
HpyF3I	CTNAG	5 cut(s)	376, 386, 521, 1098, 1286
HpySE526I	ACGT	3 cut(s)	150, 588, 666
Hsp92I	GRCGYC	5 cut(s)	38, 120, 593, 912, 1251
Hsp92II	CATG	4 cut(s)	220, 250, 799, 1336
KasI	GGCGCC	3 cut(s)	37, 119, 592
KflI	GGGWCCC	1 cut(s)	576
Ksp22I	TGATCA	1 cut(s)	1312
KspI	CCGCGG	1 cut(s)	442
Kzo9I	GATC	7 cut(s)	237, 309, 444, 540, 769, 825, 1312
LmnI	GCTCC	3 cut(s)	12, 111, 561
Lsp1109I	GCAGC	6 cut(s)	18, 64, 67, 70, 418, 1041
LweI	GCATC	1 cut(s)	1329
MaeII	ACGT	3 cut(s)	150, 588, 666
MaeIII	GTNAC	1 cut(s)	949
MalI	GATC	7 cut(s)	239, 311, 446, 542, 771, 827, 1314
MbiI	CCGCTC	2 cut(s)	492, 1026
MboI	GATC	7 cut(s)	237, 309, 444, 540, 769, 825, 1312
MboII	GAAGA	8 cut(s)	29, 205, 622, 736, 1147, 1288, 1329, 1332
MflI	RGATCY	4 cut(s)	309, 444, 540, 769
MhlI	GDGCHC	4 cut(s)	99, 522, 558, 752
MlsI	TGGCCA	1 cut(s)	162
MluCI	AATT	6 cut(s)	472, 480, 496, 703, 881, 1174
MluI	ACGCGT	1 cut(s)	963
MluNI	TGGCCA	1 cut(s)	162
Mly113I	GGCGCC	3 cut(s)	38, 120, 593
MlyI	GAGTC	1 cut(s)	1025
MmeI	TCCRAC	3 cut(s)	246, 568, 975
Mox20I	TGGCCA	1 cut(s)	162
MroXI	GAANNNNTTC	2 cut(s)	398, 965
MscI	TGGCCA	1 cut(s)	162
MseI	TTAA	1 cut(s)	479
MslI	CAYNNNNRTG	1 cut(s)	245
Msp20I	TGGCCA	1 cut(s)	162
MspA1I	CMGCKG	2 cut(s)	31, 441
MspR9I	CCNGG	6 cut(s)	9, 679, 809, 817, 991, 1006
Mva1269I	GAATGC	1 cut(s)	1376
MvnI	CGCG	6 cut(s)	234, 441, 456, 629, 965, 1256
NarI	GGCGCC	3 cut(s)	38, 120, 593
NciI	CCSGG	6 cut(s)	9, 679, 809, 817, 991, 1006
NdeII	GATC	7 cut(s)	237, 309, 444, 540, 769, 825, 1312
NlaIII	CATG	4 cut(s)	220, 250, 799, 1336
NlaIV	GGNNCC	9 cut(s)	39, 121, 208, 557, 577, 578, 594, 749, 846
NmeAIII	GCCGAG	2 cut(s)	78, 965
NmuCI	GTSAC	1 cut(s)	949
PceI	AGGCCT	1 cut(s)	1291
PcsI	WCGNNNNNNNCGW	4 cut(s)	90, 147, 327, 927
PctI	GAATGC	1 cut(s)	1376
PdmI	GAANNNNTTC	2 cut(s)	398, 965
PfeI	GAWTC	1 cut(s)	382
Ple19I	CGATCG	1 cut(s)	828
PleI	GAGTC	1 cut(s)	1024
PluTI	GGCGCC	3 cut(s)	41, 123, 596
PpsI	GAGTC	1 cut(s)	1024
Ppu21I	YACGTR	1 cut(s)	589
PpuMI	RGGWCCY	3 cut(s)	289, 576, 844
Psp124BI	GAGCTC	1 cut(s)	99
Psp5II	RGGWCCY	3 cut(s)	289, 576, 844
PspN4I	GGNNCC	9 cut(s)	39, 121, 208, 557, 577, 578, 594, 749, 846
PspPI	GGNCC	4 cut(s)	289, 576, 844, 997
PspPPI	RGGWCCY	3 cut(s)	289, 576, 844
PsrI	GAACNNNNNNTAC	2 cut(s)	674, 706
PstNI	CAGNNNCTG	1 cut(s)	31
PsuI	RGATCY	4 cut(s)	309, 444, 540, 769
PvuI	CGATCG	1 cut(s)	828
RsaI	GTAC	4 cut(s)	92, 398, 428, 665
RsaNI	GTAC	4 cut(s)	91, 397, 427, 664
RseI	CAYNNNNRTG	1 cut(s)	245
SacI	GAGCTC	1 cut(s)	99
SacII	CCGCGG	1 cut(s)	442
SaqAI	TTAA	1 cut(s)	479
Sau3AI	GATC	7 cut(s)	237, 309, 444, 540, 769, 825, 1312
Sau96I	GGNCC	4 cut(s)	289, 576, 844, 997
ScaI	AGTACT	1 cut(s)	398
SchI	GAGTC	1 cut(s)	1025
ScrFI	CCNGG	6 cut(s)	9, 679, 809, 817, 991, 1006
SduI	GDGCHC	4 cut(s)	99, 522, 558, 752
SfaNI	GCATC	1 cut(s)	1329
SfoI	GGCGCC	3 cut(s)	39, 121, 594
Sfr303I	CCGCGG	1 cut(s)	442
SgrBI	CCGCGG	1 cut(s)	442
SinI	GGWCC	4 cut(s)	289, 576, 844, 997
SmiMI	CAYNNNNRTG	1 cut(s)	245
Sse9I	AATT	6 cut(s)	472, 480, 496, 703, 881, 1174
SseBI	AGGCCT	1 cut(s)	1291
SspDI	GGCGCC	3 cut(s)	37, 119, 592
SstI	GAGCTC	1 cut(s)	99
StuI	AGGCCT	1 cut(s)	1291
StyD4I	CCNGG	6 cut(s)	7, 677, 807, 815, 989, 1004
StyI	CCWWGG	2 cut(s)	354, 1107
TaaI	ACNGT	2 cut(s)	663, 1072
TaiI	ACGT	3 cut(s)	153, 591, 669
TaqI	TCGA	6 cut(s)	362, 828, 902, 969, 1014, 1022
TasI	AATT	6 cut(s)	472, 480, 496, 703, 881, 1174
TatI	WGTACW	1 cut(s)	396
TfiI	GAWTC	1 cut(s)	382
Tru1I	TTAA	1 cut(s)	479
Tru9I	TTAA	1 cut(s)	479
TscAI	CASTG	1 cut(s)	163
TseFI	GTSAC	1 cut(s)	949
TseI	GCWGC	6 cut(s)	31, 52, 55, 58, 406, 1029
Tsp45I	GTSAC	1 cut(s)	949
TspRI	CASTG	1 cut(s)	163
VpaK11BI	GGWCC	4 cut(s)	289, 576, 844, 997
XapI	RAATTY	4 cut(s)	472, 496, 881, 1174
XmiI	GTMKAC	1 cut(s)	135
XmnI	GAANNNNTTC	2 cut(s)	398, 965
ZrmI	AGTACT	1 cut(s)	398

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.