MD03G1058000.v1.1

28S ribosomal protein S29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011212
Physical Location & Seq
Forward (+)
4669035 .. 4672456
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1058000.v1.1.491

Sequence Viewer

Length: 1359 bp
ATGTTTCGGACCCTTCTCCGACGGGCATCAGGGTCGGGCGGCCCAAGACCCGACCCATGGAGGCTGACGGCGACGATGAGCTACTCGACATCCAAGGACACCATGGCCAAGAAGGCGAAGAAGACCGGCAAATCCAAAAACGATCCCGCAGCAGCCGCCGACGACGCCTTATCGGAGGTCGACGCGGCTCTCTCCGGCCAAAATTCCCGCGCCCGCCAGCTCCAAGCCGACGAGAAGGACCGGTCGCTCGATGTGGGACCCAACGGCCGTCCTCTGTTTACTTCCACTGCGACACTCTCTCAGCTCAGCCGCAAGGACACATGCTCCTACTTCAACCTCAAGATGGAGGAATTGAACAATGTTTTGCCCGAGGGGTTGCCATTGGGGATGGTGAAGGAGTTCGAGGACGCAATGCAGAGTGCAGTGATCGTCCGCCAGAGCTTTCTCGATCTTCGTGACAATTTTCGAAGGATTGTGGACCCGCCGTTGGACTCGGTTAGCAGCAAAGGTACAAGAGCTCAAAAGCAGATTGTCTTGGACGGTCCTGTAAGCTGCGGAAAGAGTATTACTCTTGCAATGTTAGTTCAATGGGCTCGTGAGGAAGGTTGGTTGGTTTTATATGTTCCAAGAGGCAGGGAGTGGACTCATGGAGGATTCTTCTATAAAAACCCACAAACAGGTCTTTGGGACACACCTTTTCAGGCTGAAAATATTCTGAAGGATTTCCTGAAATATAACGAATCCCATTTAAAACAATTGTCGTGCCAAATATTTGATCCTATTCCCTTGGGAGAAGGTGCTGGTGTCGGGTGGATGAGAGATGGAACTGATTCCATGGCAATGCCTGAAGGTTCAACTCTATATGACCTAGTTAATTCAGGAATCAGGCACACTCATGCAGCTGTTGGAGTGGTAGTTCGTTTGAGGAAGGAATTATCTCTTGTGAAAAATATTCCCGTGCTTATTGCAATTGATCAGTATAATAACTGGTTTACATTCAGCGAGTATGAAGAGCCAGTAACTGTCCGTTCTACTCGACCAATACATGCTAAAGAACTCGCTACGGTGAAGGCTTTTAGATCTATGCGGCATGATGACATGATGGTGGGCGCATTTTCACATTCAACGGCAGTAGGAAAGCTTCGTCAAGACTTGCCGGATGTGCCTGTAGATGCTCGTGTCAACCTTTCACGCTACACTTTAGATGAAGCAGCAGCTGTTTGCCATTACTACCTTAGACAGAGGCTTATTCGCCGTGAAGCATTCACAGAGAACAACTGGAAGAAAGTATACTACTTGTCGAATGGAAATGGAGCAGAAATGAGATGGCTAGTTCCTTTGATGCGGGCTGATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAP3

Protein Analysis

453

Amino Acids

50.79

Weight (kDa)

9.05

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAP3 PF10236 169 - 371 3.3e-31 Mitochondrial ribosomal death-associated protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16870
fragaria_vesca FvH4_3g39930 FvH4_3g39930
malus_domestica MD03G1058000.v1.1 MD11G1060300.v1.1
prunus_persica Prupe.6G046100_v2.0.a1
pyrus_communis pycom03g04630 pycom11g04980
rosa_chinensis RchiOBHm_Chr5g0071911 RchiOBHm_Chr5g0071921
rosa_laevigata RLG00000036262
rosa_multiflora Rmu_sc0002149.1_g000018
rosa_roxburghii Rroxscaffold_1G00009260
rosa_rugosa Rorug05G0415600 Rorug05G0415700
rosa_samantha Rh5AG471600 Rh5BG491500 Rh5CG515400 Rh5DG503000
rosa_wichuraiana Rw5G043830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 180, 1290
AccII CGCG 2 cut(s) 185, 210
AclWI GGATC 2 cut(s) 137, 770
AcoI YGGCCR 3 cut(s) 105, 196, 265
AcsI RAATTY 1 cut(s) 202
AcuI CTGAAG 2 cut(s) 737, 867
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 511
AfiI CCNNNNNNNGG 4 cut(s) 57, 343, 487, 677
AgeI ACCGGT 1 cut(s) 240
AgsI TTSAA 5 cut(s) 334, 355, 587, 855, 1125
AloI GAACNNNNNNTCC 2 cut(s) 900, 932
AluBI AGCT 9 cut(s) 81, 220, 304, 441, 518, 552, 902, 1141, 1217
AluI AGCT 9 cut(s) 81, 220, 304, 441, 518, 552, 902, 1141, 1217
Alw21I GWGCWC 1 cut(s) 520
AlwI GGATC 2 cut(s) 137, 770
AlwNI CAGNNNCTG 2 cut(s) 1022, 1217
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 4 cut(s) 40, 105, 196, 265
ApeKI GCWGC 7 cut(s) 149, 152, 501, 552, 899, 1211, 1214
ApoI RAATTY 1 cut(s) 202
AsiGI ACCGGT 1 cut(s) 240
Asp700I GAANNNNTTC 4 cut(s) 398, 711, 722, 829
AspLEI GCGC 2 cut(s) 212, 1112
AspS9I GGNCC 6 cut(s) 9, 41, 238, 257, 478, 542
AsuHPI GGTGA 2 cut(s) 403, 1078
AsuII TTCGAA 1 cut(s) 466
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 5 cut(s) 9, 238, 257, 478, 542
BalI TGGCCA 1 cut(s) 107
BanII GRGCYC 2 cut(s) 520, 595
BarI GAAGNNNNNNTAC 4 cut(s) 1125, 1157, 1274, 1306
BauI CACGAG 2 cut(s) 594, 1176
BbsI GAAGAC 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 7 cut(s) 161, 164, 513, 539, 911, 1223, 1226
BccI CCATC 5 cut(s) 337, 382, 815, 1096, 1320
BceAI ACGGC 6 cut(s) 84, 252, 280, 469, 1143, 1239
BcgI CGANNNNNNTGC 2 cut(s) 15, 49
BclI TGATCA 1 cut(s) 973
BfaI CTAG 2 cut(s) 869, 1331
BfmI CTRYAG 1 cut(s) 1167
BglI GCCNNNNNGGC 1 cut(s) 113
BglII AGATCT 1 cut(s) 1079
BlpI GCTNAGC 1 cut(s) 305
Bme18I GGWCC 5 cut(s) 9, 238, 257, 478, 542
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 6 cut(s) 9, 41, 238, 257, 478, 542
BmiI GGNNCC 4 cut(s) 11, 258, 259, 480
BmsI GCATC 3 cut(s) 35, 1162, 1332
BpiI GAAGAC 1 cut(s) 128
BplI GAGNNNNNCTC 2 cut(s) 553, 585
Bpu1102I GCTNAGC 1 cut(s) 305
Bpu14I TTCGAA 1 cut(s) 466
BpuEI CTTGAG 1 cut(s) 323
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 6 cut(s) 56, 93, 102, 369, 786, 834
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 4 cut(s) 308, 338, 900, 930
Bsc4I CCNNNNNNNGG 4 cut(s) 57, 343, 487, 677
Bse118I RCCGGY 2 cut(s) 125, 240
Bse1I ACTGG 3 cut(s) 992, 1016, 1283
Bse3DI GCAATG 3 cut(s) 417, 582, 846
BseDI CCNNGG 6 cut(s) 56, 93, 102, 369, 786, 834
BseGI GGATG 4 cut(s) 89, 393, 819, 1165
BseLI CCNNNNNNNGG 4 cut(s) 57, 343, 487, 677
BseMI GCAATG 3 cut(s) 417, 582, 846
BseMII CTCAG 2 cut(s) 314, 319
BseNI ACTGG 3 cut(s) 992, 1016, 1283
BseX3I CGGCCG 1 cut(s) 265
BseXI GCAGC 7 cut(s) 161, 164, 513, 539, 911, 1223, 1226
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 2 cut(s) 185, 210
Bsh1285I CGRYCG 2 cut(s) 245, 268
BshFI GGCC 4 cut(s) 42, 107, 198, 267
BshTI ACCGGT 1 cut(s) 240
BsiEI CGRYCG 2 cut(s) 245, 268
BsiHKAI GWGCWC 1 cut(s) 520
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 4 cut(s) 126, 195, 241, 1157
BslFI GGGAC 2 cut(s) 270, 701
BslI CCNNNNNNNGG 4 cut(s) 57, 343, 487, 677
BsmFI GGGAC 2 cut(s) 270, 701
BsmI GAATGC 1 cut(s) 1262
BsnI GGCC 4 cut(s) 42, 107, 198, 267
BsoBI CYCGRG 1 cut(s) 368
Bsp119I TTCGAA 1 cut(s) 466
Bsp1286I GDGCHC 2 cut(s) 520, 595
Bsp143I GATC 6 cut(s) 142, 426, 448, 775, 973, 1079
Bsp1720I GCTNAGC 1 cut(s) 305
Bsp19I CCATGG 3 cut(s) 56, 102, 834
BspANI GGCC 4 cut(s) 42, 107, 198, 267
BspCNI CTCAG 2 cut(s) 313, 318
BspFNI CGCG 2 cut(s) 185, 210
BspLI GGNNCC 4 cut(s) 11, 258, 259, 480
BspPI GGATC 2 cut(s) 137, 770
BspQI GCTCTTC 1 cut(s) 1005
BspT104I TTCGAA 1 cut(s) 466
BsrDI GCAATG 3 cut(s) 417, 582, 846
BsrFI RCCGGY 2 cut(s) 125, 240
BsrI ACTGG 3 cut(s) 992, 1016, 1283
BssAI RCCGGY 2 cut(s) 125, 240
BssECI CCNNGG 6 cut(s) 56, 93, 102, 369, 786, 834
BssMI GATC 6 cut(s) 142, 426, 448, 775, 973, 1079
BssNAI GTATAC 1 cut(s) 1291
BssNI GRCGYC 1 cut(s) 165
BssSI CACGAG 2 cut(s) 594, 1176
BssT1I CCWWGG 5 cut(s) 56, 93, 102, 786, 834
Bst1107I GTATAC 1 cut(s) 1291
Bst2BI CACGAG 2 cut(s) 594, 1176
Bst4CI ACNGT 3 cut(s) 542, 1024, 1066
Bst6I CTCTTC 1 cut(s) 1005
BstACI GRCGYC 1 cut(s) 165
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 3 cut(s) 214, 218, 1347
BstDEI CTNAG 3 cut(s) 300, 305, 1235
BstDSI CCRYGG 3 cut(s) 56, 102, 834
BstF5I GGATG 4 cut(s) 89, 393, 819, 1165
BstFNI CGCG 2 cut(s) 185, 210
BstHHI GCGC 2 cut(s) 212, 1112
BstKTI GATC 6 cut(s) 145, 429, 451, 778, 976, 1082
BstMBI GATC 6 cut(s) 142, 426, 448, 775, 973, 1079
BstMCI CGRYCG 2 cut(s) 245, 268
BstMWI GCNNNNNNNGC 4 cut(s) 113, 155, 164, 1162
BstNSI RCATGY 2 cut(s) 324, 1049
BstSFI CTRYAG 1 cut(s) 1167
BstUI CGCG 2 cut(s) 185, 210
BstV1I GCAGC 7 cut(s) 161, 164, 513, 539, 911, 1223, 1226
BstV2I GAAGAC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 1079
BstYI RGATCY 1 cut(s) 1079
BstZ17I GTATAC 1 cut(s) 1291
BstZI CGGCCG 1 cut(s) 265
BsuRI GGCC 4 cut(s) 42, 107, 198, 267
BtgI CCRYGG 3 cut(s) 56, 102, 834
BtsCI GGATG 4 cut(s) 89, 393, 819, 1165
BtsI GCAGTG 2 cut(s) 285, 429
BtsIMutI CAGTG 2 cut(s) 285, 429
Cac8I GCNNGC 3 cut(s) 214, 218, 1347
CaiI CAGNNNCTG 2 cut(s) 1022, 1217
CfoI GCGC 2 cut(s) 212, 1112
Cfr10I RCCGGY 2 cut(s) 125, 240
Cfr13I GGNCC 6 cut(s) 9, 41, 238, 257, 478, 542
CseI GACGC 3 cut(s) 173, 191, 416
Csp6I GTAC 1 cut(s) 510
CspAI ACCGGT 1 cut(s) 240
CviAII CATG 9 cut(s) 57, 103, 321, 647, 835, 896, 1046, 1091, 1099
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 3 cut(s) 300, 305, 1235
DpnI GATC 6 cut(s) 144, 428, 450, 777, 975, 1081
DpnII GATC 6 cut(s) 142, 426, 448, 775, 973, 1079
DraI TTTAAA 1 cut(s) 750
EaeI YGGCCR 3 cut(s) 105, 196, 265
EagI CGGCCG 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 1005
EarI CTCTTC 1 cut(s) 1005
EciI GGCGGA 1 cut(s) 422
Ecl136II GAGCTC 1 cut(s) 518
EclXI CGGCCG 1 cut(s) 265
Eco130I CCWWGG 5 cut(s) 56, 93, 102, 786, 834
Eco24I GRGCYC 2 cut(s) 520, 595
Eco47I GGWCC 5 cut(s) 9, 238, 257, 478, 542
Eco52I CGGCCG 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 518
Eco57I CTGAAG 2 cut(s) 737, 867
Eco88I CYCGRG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 518
EcoO109I RGGNCCY 1 cut(s) 257
EcoT14I CCWWGG 5 cut(s) 56, 93, 102, 786, 834
EcoT38I GRGCYC 2 cut(s) 520, 595
ErhI CCWWGG 5 cut(s) 56, 93, 102, 786, 834
FaeI CATG 9 cut(s) 60, 106, 324, 650, 838, 899, 1049, 1094, 1102
FaqI GGGAC 2 cut(s) 270, 701
FatI CATG 9 cut(s) 56, 102, 320, 646, 834, 895, 1045, 1090, 1098
FauI CCCGC 5 cut(s) 154, 215, 221, 489, 1338
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 2 cut(s) 180, 1290
FokI GGATG 4 cut(s) 76, 400, 826, 1172
FriOI GRGCYC 2 cut(s) 520, 595
FspBI CTAG 2 cut(s) 869, 1331
GlaI GCGC 2 cut(s) 211, 1111
HaeIII GGCC 4 cut(s) 42, 107, 198, 267
HapII CCGG 4 cut(s) 126, 195, 241, 1157
HgaI GACGC 3 cut(s) 173, 191, 416
HhaI GCGC 2 cut(s) 212, 1112
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 9 cut(s) 60, 106, 324, 650, 838, 899, 1049, 1094, 1102
Hin6I GCGC 2 cut(s) 210, 1110
HinP1I GCGC 2 cut(s) 210, 1110
HincII GTYRAC 2 cut(s) 181, 1183
HindII GTYRAC 2 cut(s) 181, 1183
HindIII AAGCTT 1 cut(s) 1139
HinfI GANTC 6 cut(s) 491, 643, 654, 740, 830, 882
HpaII CCGG 4 cut(s) 126, 195, 241, 1157
HphI GGTGA 2 cut(s) 403, 1078
Hpy166II GTNNAC 7 cut(s) 181, 279, 478, 642, 993, 1183, 1291
Hpy188I TCNGA 4 cut(s) 9, 20, 175, 717
Hpy188III TCNNGA 7 cut(s) 340, 446, 455, 596, 727, 879, 1148
Hpy8I GTNNAC 7 cut(s) 181, 279, 478, 642, 993, 1183, 1291
Hpy99I CGWCG 6 cut(s) 24, 76, 164, 167, 185, 233
HpyCH4III ACNGT 3 cut(s) 542, 1024, 1066
HpyCH4V TGCA 5 cut(s) 415, 422, 575, 899, 968
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 155, 164, 1162
HpyF3I CTNAG 3 cut(s) 300, 305, 1235
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 9 cut(s) 60, 106, 324, 650, 838, 899, 1049, 1094, 1102
HspAI GCGC 2 cut(s) 210, 1110
KflI GGGWCCC 1 cut(s) 257
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 6 cut(s) 142, 426, 448, 775, 973, 1079
LguI GCTCTTC 1 cut(s) 1005
LmnI GCTCC 3 cut(s) 225, 329, 1313
Lsp1109I GCAGC 7 cut(s) 161, 164, 513, 539, 911, 1223, 1226
LweI GCATC 3 cut(s) 35, 1162, 1332
MaeI CTAG 2 cut(s) 869, 1331
MaeIII GTNAC 2 cut(s) 455, 1018
MalI GATC 6 cut(s) 144, 428, 450, 777, 975, 1081
MboI GATC 6 cut(s) 142, 426, 448, 775, 973, 1079
MboII GAAGA 6 cut(s) 130, 133, 443, 649, 1022, 1294
MfeI CAATTG 2 cut(s) 755, 969
MflI RGATCY 1 cut(s) 1079
MhlI GDGCHC 2 cut(s) 520, 595
MlsI TGGCCA 1 cut(s) 107
MluCI AATT 7 cut(s) 202, 350, 460, 755, 874, 932, 969
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 2 cut(s) 485, 637
MmeI TCCRAC 3 cut(s) 43, 468, 886
Mox20I TGGCCA 1 cut(s) 107
MroXI GAANNNNTTC 4 cut(s) 398, 711, 722, 829
MscI TGGCCA 1 cut(s) 107
MseI TTAA 2 cut(s) 749, 873
MslI CAYNNNNRTG 3 cut(s) 839, 894, 1103
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 2 cut(s) 902, 1217
MspI CCGG 4 cut(s) 126, 195, 241, 1157
MunI CAATTG 2 cut(s) 755, 969
Mva1269I GAATGC 1 cut(s) 1262
MvnI CGCG 2 cut(s) 185, 210
MwoI GCNNNNNNNGC 4 cut(s) 113, 155, 164, 1162
NcoI CCATGG 3 cut(s) 56, 102, 834
NdeII GATC 6 cut(s) 142, 426, 448, 775, 973, 1079
NlaIII CATG 9 cut(s) 60, 106, 324, 650, 838, 899, 1049, 1094, 1102
NlaIV GGNNCC 4 cut(s) 11, 258, 259, 480
NmuCI GTSAC 1 cut(s) 455
NspI RCATGY 2 cut(s) 324, 1049
NspV TTCGAA 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 1005
PctI GAATGC 1 cut(s) 1262
PdmI GAANNNNTTC 4 cut(s) 398, 711, 722, 829
PfeI GAWTC 4 cut(s) 654, 740, 830, 882
PinAI ACCGGT 1 cut(s) 240
PleI GAGTC 2 cut(s) 485, 637
PpsI GAGTC 2 cut(s) 485, 637
PpuMI RGGWCCY 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 520
Psp5II RGGWCCY 1 cut(s) 257
PspN4I GGNNCC 4 cut(s) 11, 258, 259, 480
PspPI GGNCC 6 cut(s) 9, 41, 238, 257, 478, 542
PspPPI RGGWCCY 1 cut(s) 257
PstNI CAGNNNCTG 2 cut(s) 1022, 1217
PsuI RGATCY 1 cut(s) 1079
PvuII CAGCTG 2 cut(s) 902, 1217
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
RseI CAYNNNNRTG 3 cut(s) 839, 894, 1103
SacI GAGCTC 1 cut(s) 520
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 1005
SaqAI TTAA 2 cut(s) 749, 873
Sau3AI GATC 6 cut(s) 142, 426, 448, 775, 973, 1079
Sau96I GGNCC 6 cut(s) 9, 41, 238, 257, 478, 542
SchI GAGTC 2 cut(s) 485, 637
SduI GDGCHC 2 cut(s) 520, 595
SfaNI GCATC 3 cut(s) 35, 1162, 1332
SfcI CTRYAG 1 cut(s) 1167
SfuI TTCGAA 1 cut(s) 466
SinI GGWCC 5 cut(s) 9, 238, 257, 478, 542
SmiMI CAYNNNNRTG 3 cut(s) 839, 894, 1103
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 7 cut(s) 202, 350, 460, 755, 874, 932, 969
SspI AATATT 3 cut(s) 712, 771, 952
SspMI CTAG 2 cut(s) 869, 1331
SstI GAGCTC 1 cut(s) 520
StyI CCWWGG 5 cut(s) 56, 93, 102, 786, 834
TaaI ACNGT 3 cut(s) 542, 1024, 1066
TaqI TCGA 8 cut(s) 86, 180, 249, 402, 447, 466, 1036, 1301
TasI AATT 7 cut(s) 202, 350, 460, 755, 874, 932, 969
TauI GCSGC 5 cut(s) 42, 158, 188, 312, 1090
TfiI GAWTC 4 cut(s) 654, 740, 830, 882
Tru1I TTAA 2 cut(s) 749, 873
Tru9I TTAA 2 cut(s) 749, 873
TscAI CASTG 2 cut(s) 292, 429
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 7 cut(s) 149, 152, 501, 552, 899, 1211, 1214
Tsp45I GTSAC 1 cut(s) 455
TspDTI ATGAA 2 cut(s) 1023, 1221
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 2 cut(s) 292, 429
VpaK11BI GGWCC 5 cut(s) 9, 238, 257, 478, 542
XapI RAATTY 1 cut(s) 202
XceI RCATGY 2 cut(s) 324, 1049
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 2 cut(s) 180, 1290
XmnI GAANNNNTTC 4 cut(s) 398, 711, 722, 829
XspI CTAG 2 cut(s) 869, 1331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.