Prupe.6G046100_v2.0.a1

28S ribosomal protein S29

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011212
Physical Location & Seq
Reverse (-)
3334384 .. 3337899
3516 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G046100.1

Sequence Viewer

Length: 1374 bp
ATGTTGCGGACCCTTCTTCGACGGGCAACGGGGTCGGGTTGCCCAAGGCATGACCCGTGGAGGCTCACGGCGACGATGAGCTACTCGTCCAAGGACAAGAAATCGACGGTGAAGAAGGCGAAGAAGGGCAAGGACAAAAACGACCCTGCAGCCGCAACAGACGACGCCGTTTCGGACGTCGACGCGGCTCTCTTCGACGAAAAGGCTCGTGCTCGCCGGCTACAAGCCGATGAAAATGATCCGTCGCTCGATGTGGGGCCCAATGGCCGGCCTCTGTTCACTGCCACTCCGACACTCTCTCAGCTCACGCGCAAGGACGCCTGCTCCTACTTCAAGCTCAAGATGGAGGAATTGAACAATGTGTTGCCCGAGGGGTTGCCGTTGGGGATGGTGAAGGAGTTTGAGGACGCAATGCAGAGCGCCGTGCTAGTTCGCCAGAGCTTTCTTGATCTCCGAGATAATTTTCGATGGATCGTGGATCCTCCATTGCACTCTTCTACCACCAAAGGTACAAAAGTTAGAAAGCAGATTGTGTTGGACGGTCCTGTAAGTTGTGGAAAGAGTATTGCTCTTTCAATGTTAGTTCAATGGGCTCGCGGGGAAGGTTGGTTGGTGTTATATGTCCCAAGAGGCAGGGACTGGACTTATGGAGGCTTTTTCTATAAAAACCCACAAACAGGTCTTTGGGACACACCTGTTCAGGCTGAAAATATTCTGAAGGACTTCCTGAAATTTAATGAATCCCGTTTAAAACAATTGTTCTGCCAAATATTTGATCCTATTCCATTGGGAGAGGGTGCTGGTGTTGGATGGATGAAAGATGGAATTGATACCATGGCAATGCCTGATGGTTCAACTCTGTATGACCTGGTTGATACAGGAATCAAGCACACTCATGCAGCTGTTGGAGTGGTAGTTCGTTTGAGGAAGGAGTTATCTCTTGTGAAAGATATCCCCGTGCTTATTGCAATTGATCAGTATAATAGCTGGTTTACGTTCAGTGAGTATGAAGAGCCAGTAACTGTTCGTTCTACTCGACCAATACATGCTAAAGAACTTGCTACGGTGAAGGCTTTTAGATCTATGCGACATGATGACTTGATGGTGGGCGCGTTTTCTCATTCAACAGCAGTAGGAAAGCTTCGTCAAGACTTGCCAGAAGTGCCTACAGATGCTCGCGTGAACTTTCCTCGCTACACTTTAGATGAAGCAGCAGCTGTTTGCCATTACTACCTTAGACAGAGGCTTATACGCCGTGAAGCATTCACGGAGGAGAACTGGAAGAAAATATACTATTTGTCTAATGGAAATGGAGCAGAAATTAGATGGTTAGTGCCTTTGATGCGGGGAGATGGTATGCAGGCAGATGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DAP3

Protein Analysis

458

Amino Acids

51.5

Weight (kDa)

8.85

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011212)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16870
fragaria_vesca FvH4_3g39930 FvH4_3g39930
malus_domestica MD03G1058000.v1.1 MD11G1060300.v1.1
prunus_persica Prupe.6G046100_v2.0.a1
pyrus_communis pycom03g04630 pycom11g04980
rosa_chinensis RchiOBHm_Chr5g0071911 RchiOBHm_Chr5g0071921
rosa_laevigata RLG00000036262
rosa_multiflora Rmu_sc0002149.1_g000018
rosa_roxburghii Rroxscaffold_1G00009260
rosa_rugosa Rorug05G0415600 Rorug05G0415700
rosa_samantha Rh5AG471600 Rh5BG491500 Rh5CG515400 Rh5DG503000
rosa_wichuraiana Rw5G043830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AccI GTMKAC 1 cut(s) 180
AccII CGCG 5 cut(s) 185, 310, 597, 1112, 1179
AciI CCGC 5 cut(s) 7, 153, 185, 597, 1345
AclWI GGATC 5 cut(s) 233, 473, 479, 486, 770
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 1 cut(s) 737
AcyI GRCGYC 3 cut(s) 165, 177, 318
AfaI GTAC 1 cut(s) 511
AfiI CCNNNNNNNGG 2 cut(s) 267, 677
AgsI TTSAA 6 cut(s) 334, 355, 576, 587, 855, 1125
AjnI CCWGG 1 cut(s) 867
AloI GAACNNNNNNTCC 2 cut(s) 900, 932
AluBI AGCT 8 cut(s) 81, 304, 337, 441, 902, 987, 1141, 1217
AluI AGCT 8 cut(s) 81, 304, 337, 441, 902, 987, 1141, 1217
Alw21I GWGCWC 1 cut(s) 214
AlwI GGATC 5 cut(s) 233, 473, 479, 486, 770
AlwNI CAGNNNCTG 3 cut(s) 639, 1022, 1217
Ama87I CYCGRG 1 cut(s) 368
AoxI GGCC 3 cut(s) 257, 265, 269
ApaI GGGCCC 1 cut(s) 261
ApeKI GCWGC 4 cut(s) 149, 899, 1211, 1214
ApoI RAATTY 1 cut(s) 731
Asp700I GAANNNNTTC 2 cut(s) 711, 722
AspLEI GCGC 3 cut(s) 312, 422, 1112
AspS9I GGNCC 4 cut(s) 9, 257, 258, 542
AsuHPI GGTGA 3 cut(s) 121, 403, 1078
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 2 cut(s) 9, 542
BaeGI GKGCMC 1 cut(s) 261
BaeI ACNNNNGTAYC 2 cut(s) 501, 534
BamHI GGATCC 1 cut(s) 478
BanII GRGCYC 2 cut(s) 261, 595
BarI GAAGNNNNNNTAC 4 cut(s) 1125, 1157, 1274, 1306
BauI CACGAG 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 4 cut(s) 161, 911, 1223, 1226
BceAI ACGGC 5 cut(s) 84, 152, 364, 407, 1239
BcgI CGANNNNNNTGC 2 cut(s) 15, 49
BciT130I CCWGG 1 cut(s) 869
BclI TGATCA 1 cut(s) 973
BfaI CTAG 1 cut(s) 428
BfmI CTRYAG 2 cut(s) 147, 1167
BfoI RGCGCY 1 cut(s) 423
BglII AGATCT 1 cut(s) 1079
BisI GCNGC 6 cut(s) 150, 153, 186, 900, 1212, 1215
BlsI GCNGC 6 cut(s) 151, 154, 187, 901, 1213, 1216
Bme1390I CCNGG 1 cut(s) 869
Bme18I GGWCC 2 cut(s) 9, 542
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 4 cut(s) 9, 257, 258, 542
BmiI GGNNCC 4 cut(s) 11, 258, 259, 480
BmrFI CCNGG 1 cut(s) 869
BmsI GCATC 2 cut(s) 1162, 1332
BplI GAGNNNNNCTC 2 cut(s) 553, 585
BpuEI CTTGAG 1 cut(s) 323
BsaHI GRCGYC 3 cut(s) 165, 177, 318
BsaJI CCNNGG 5 cut(s) 44, 56, 90, 369, 834
BsaXI ACNNNNNCTCC 6 cut(s) 271, 301, 308, 338, 900, 930
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 677
Bse118I RCCGGY 2 cut(s) 216, 267
Bse1I ACTGG 3 cut(s) 644, 1016, 1283
Bse3DI GCAATG 3 cut(s) 417, 485, 846
BseBI CCWGG 1 cut(s) 869
BseDI CCNNGG 5 cut(s) 44, 56, 90, 369, 834
BseGI GGATG 3 cut(s) 393, 815, 819
BseLI CCNNNNNNNGG 2 cut(s) 267, 677
BseMI GCAATG 3 cut(s) 417, 485, 846
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 3 cut(s) 644, 1016, 1283
BseRI GAGGAG 1 cut(s) 1286
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 4 cut(s) 161, 911, 1223, 1226
Bsh1236I CGCG 5 cut(s) 185, 310, 597, 1112, 1179
BshFI GGCC 3 cut(s) 259, 267, 271
BsiHKAI GWGCWC 1 cut(s) 214
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 2 cut(s) 217, 268
BslFI GGGAC 3 cut(s) 608, 650, 701
BslI CCNNNNNNNGG 2 cut(s) 267, 677
BsmFI GGGAC 3 cut(s) 608, 650, 701
BsmI GAATGC 1 cut(s) 1262
BsnI GGCC 3 cut(s) 259, 267, 271
BsoBI CYCGRG 1 cut(s) 368
Bsp120I GGGCCC 1 cut(s) 257
Bsp1286I GDGCHC 3 cut(s) 214, 261, 595
Bsp143I GATC 7 cut(s) 238, 448, 471, 478, 775, 973, 1079
Bsp19I CCATGG 1 cut(s) 834
BspACI CCGC 5 cut(s) 7, 153, 185, 597, 1345
BspANI GGCC 3 cut(s) 259, 267, 271
BspCNI CTCAG 1 cut(s) 313
BspFNI CGCG 5 cut(s) 185, 310, 597, 1112, 1179
BspLI GGNNCC 4 cut(s) 11, 258, 259, 480
BspMAI CTGCAG 1 cut(s) 151
BspPI GGATC 5 cut(s) 233, 473, 479, 486, 770
BspQI GCTCTTC 1 cut(s) 1005
BsrDI GCAATG 3 cut(s) 417, 485, 846
BsrFI RCCGGY 2 cut(s) 216, 267
BsrI ACTGG 3 cut(s) 644, 1016, 1283
BssAI RCCGGY 2 cut(s) 216, 267
BssECI CCNNGG 5 cut(s) 44, 56, 90, 369, 834
BssMI GATC 7 cut(s) 238, 448, 471, 478, 775, 973, 1079
BssNI GRCGYC 3 cut(s) 165, 177, 318
BssSI CACGAG 1 cut(s) 207
BssT1I CCWWGG 3 cut(s) 44, 90, 834
Bst2BI CACGAG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 869
Bst4CI ACNGT 4 cut(s) 109, 542, 1024, 1066
Bst6I CTCTTC 3 cut(s) 197, 499, 1005
BstACI GRCGYC 3 cut(s) 165, 177, 318
BstC8I GCNNGC 7 cut(s) 214, 218, 269, 322, 595, 1177, 1362
BstDEI CTNAG 2 cut(s) 300, 1235
BstDSI CCRYGG 2 cut(s) 56, 834
BstF5I GGATG 3 cut(s) 393, 815, 819
BstFNI CGCG 5 cut(s) 185, 310, 597, 1112, 1179
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 3 cut(s) 312, 422, 1112
BstKTI GATC 7 cut(s) 241, 451, 474, 481, 778, 976, 1082
BstMBI GATC 7 cut(s) 238, 448, 471, 478, 775, 973, 1079
BstMWI GCNNNNNNNGC 2 cut(s) 1162, 1342
BstNI CCWGG 1 cut(s) 869
BstNSI RCATGY 1 cut(s) 1049
BstSCI CCNGG 1 cut(s) 867
BstSFI CTRYAG 2 cut(s) 147, 1167
BstSLI GKGCMC 1 cut(s) 261
BstUI CGCG 5 cut(s) 185, 310, 597, 1112, 1179
BstV1I GCAGC 4 cut(s) 161, 911, 1223, 1226
BstX2I RGATCY 2 cut(s) 478, 1079
BstYI RGATCY 2 cut(s) 478, 1079
BsuRI GGCC 3 cut(s) 259, 267, 271
BtgI CCRYGG 2 cut(s) 56, 834
BtsCI GGATG 3 cut(s) 393, 815, 819
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 279, 1006
Cac8I GCNNGC 7 cut(s) 214, 218, 269, 322, 595, 1177, 1362
CaiI CAGNNNCTG 3 cut(s) 639, 1022, 1217
CfoI GCGC 3 cut(s) 312, 422, 1112
Cfr10I RCCGGY 2 cut(s) 216, 267
Cfr13I GGNCC 4 cut(s) 9, 257, 258, 542
CseI GACGC 4 cut(s) 173, 191, 326, 416
CsiI ACCWGGT 1 cut(s) 867
Csp6I GTAC 1 cut(s) 510
CviAII CATG 5 cut(s) 50, 835, 896, 1046, 1091
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 2 cut(s) 300, 1235
DpnI GATC 7 cut(s) 240, 450, 473, 480, 777, 975, 1081
DpnII GATC 7 cut(s) 238, 448, 471, 478, 775, 973, 1079
DraI TTTAAA 1 cut(s) 750
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 3 cut(s) 197, 499, 1005
EarI CTCTTC 3 cut(s) 197, 499, 1005
Eco130I CCWWGG 3 cut(s) 44, 90, 834
Eco24I GRGCYC 2 cut(s) 261, 595
Eco32I GATATC 1 cut(s) 952
Eco47I GGWCC 2 cut(s) 9, 542
Eco57I CTGAAG 1 cut(s) 737
Eco88I CYCGRG 1 cut(s) 368
EcoO109I RGGNCCY 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 867
EcoRV GATATC 1 cut(s) 952
EcoT14I CCWWGG 3 cut(s) 44, 90, 834
EcoT38I GRGCYC 2 cut(s) 261, 595
ErhI CCWWGG 3 cut(s) 44, 90, 834
FaeI CATG 5 cut(s) 53, 838, 899, 1049, 1094
FaqI GGGAC 3 cut(s) 608, 650, 701
FatI CATG 5 cut(s) 49, 834, 895, 1045, 1090
FauI CCCGC 2 cut(s) 590, 1338
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 6 cut(s) 150, 153, 186, 900, 1212, 1215
FokI GGATG 3 cut(s) 400, 822, 826
FriOI GRGCYC 2 cut(s) 261, 595
FseI GGCCGGCC 1 cut(s) 271
Fsp4HI GCNGC 6 cut(s) 150, 153, 186, 900, 1212, 1215
FspBI CTAG 1 cut(s) 428
GlaI GCGC 3 cut(s) 311, 421, 1111
GluI GCNGC 6 cut(s) 150, 153, 186, 900, 1212, 1215
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 3 cut(s) 259, 267, 271
HapII CCGG 2 cut(s) 217, 268
HgaI GACGC 4 cut(s) 173, 191, 326, 416
HhaI GCGC 3 cut(s) 312, 422, 1112
Hin1I GRCGYC 3 cut(s) 165, 177, 318
Hin1II CATG 5 cut(s) 53, 838, 899, 1049, 1094
Hin6I GCGC 3 cut(s) 310, 420, 1110
HinP1I GCGC 3 cut(s) 310, 420, 1110
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HindIII AAGCTT 1 cut(s) 1139
HinfI GANTC 2 cut(s) 740, 882
HpaII CCGG 2 cut(s) 217, 268
HphI GGTGA 3 cut(s) 121, 403, 1078
Hpy166II GTNNAC 4 cut(s) 181, 279, 993, 1183
Hpy188I TCNGA 4 cut(s) 175, 291, 455, 717
Hpy188III TCNNGA 4 cut(s) 340, 446, 727, 1148
Hpy8I GTNNAC 4 cut(s) 181, 279, 993, 1183
Hpy99I CGWCG 8 cut(s) 24, 76, 109, 167, 182, 185, 200, 247
HpyAV CCTTC 8 cut(s) 23, 109, 118, 388, 596, 712, 922, 1063
HpyCH4III ACNGT 4 cut(s) 109, 542, 1024, 1066
HpyCH4IV ACGT 2 cut(s) 177, 995
HpyCH4V TGCA 6 cut(s) 149, 415, 490, 899, 968, 1360
HpyF10VI GCNNNNNNNGC 2 cut(s) 1162, 1342
HpyF3I CTNAG 2 cut(s) 300, 1235
HpySE526I ACGT 2 cut(s) 177, 995
Hsp92I GRCGYC 3 cut(s) 165, 177, 318
Hsp92II CATG 5 cut(s) 53, 838, 899, 1049, 1094
HspAI GCGC 3 cut(s) 310, 420, 1110
KroI GCCGGC 2 cut(s) 216, 267
KroNI GCCGGC 2 cut(s) 218, 269
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 7 cut(s) 238, 448, 471, 478, 775, 973, 1079
LguI GCTCTTC 1 cut(s) 1005
LmnI GCTCC 2 cut(s) 329, 1313
Lsp1109I GCAGC 4 cut(s) 161, 911, 1223, 1226
LweI GCATC 2 cut(s) 1162, 1332
MabI ACCWGGT 1 cut(s) 867
MaeI CTAG 1 cut(s) 428
MaeII ACGT 2 cut(s) 177, 995
MaeIII GTNAC 1 cut(s) 1018
MalI GATC 7 cut(s) 240, 450, 473, 480, 777, 975, 1081
MboI GATC 7 cut(s) 238, 448, 471, 478, 775, 973, 1079
MboII GAAGA 7 cut(s) 8, 124, 133, 184, 486, 1022, 1294
MfeI CAATTG 2 cut(s) 755, 969
MflI RGATCY 2 cut(s) 478, 1079
MhlI GDGCHC 3 cut(s) 214, 261, 595
MluCI AATT 7 cut(s) 350, 460, 731, 755, 825, 969, 1320
MmeI TCCRAC 4 cut(s) 314, 516, 787, 886
MroNI GCCGGC 2 cut(s) 216, 267
MroXI GAANNNNTTC 2 cut(s) 711, 722
MseI TTAA 2 cut(s) 735, 749
MslI CAYNNNNRTG 2 cut(s) 839, 894
MspA1I CMGCKG 2 cut(s) 902, 1217
MspI CCGG 2 cut(s) 217, 268
MspR9I CCNGG 1 cut(s) 869
MunI CAATTG 2 cut(s) 755, 969
Mva1269I GAATGC 1 cut(s) 1262
MvaI CCWGG 1 cut(s) 869
MvnI CGCG 5 cut(s) 185, 310, 597, 1112, 1179
MwoI GCNNNNNNNGC 2 cut(s) 1162, 1342
NaeI GCCGGC 2 cut(s) 218, 269
NcoI CCATGG 1 cut(s) 834
NdeII GATC 7 cut(s) 238, 448, 471, 478, 775, 973, 1079
NgoMIV GCCGGC 2 cut(s) 216, 267
NlaIII CATG 5 cut(s) 53, 838, 899, 1049, 1094
NlaIV GGNNCC 4 cut(s) 11, 258, 259, 480
NspI RCATGY 1 cut(s) 1049
PciSI GCTCTTC 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 1 cut(s) 1033
PctI GAATGC 1 cut(s) 1262
PdiI GCCGGC 2 cut(s) 218, 269
PdmI GAANNNNTTC 2 cut(s) 711, 722
PfeI GAWTC 2 cut(s) 740, 882
PkrI GCNGC 6 cut(s) 151, 154, 187, 901, 1213, 1216
Psp6I CCWGG 1 cut(s) 867
PspGI CCWGG 1 cut(s) 867
PspN4I GGNNCC 4 cut(s) 11, 258, 259, 480
PspOMI GGGCCC 1 cut(s) 257
PspPI GGNCC 4 cut(s) 9, 257, 258, 542
PstI CTGCAG 1 cut(s) 151
PstNI CAGNNNCTG 3 cut(s) 639, 1022, 1217
PsuI RGATCY 2 cut(s) 478, 1079
PvuII CAGCTG 2 cut(s) 902, 1217
RigI GGCCGGCC 1 cut(s) 271
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
RseI CAYNNNNRTG 2 cut(s) 839, 894
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 1005
SaqAI TTAA 2 cut(s) 735, 749
SatI GCNGC 6 cut(s) 150, 153, 186, 900, 1212, 1215
Sau3AI GATC 7 cut(s) 238, 448, 471, 478, 775, 973, 1079
Sau96I GGNCC 4 cut(s) 9, 257, 258, 542
ScrFI CCNGG 1 cut(s) 869
SduI GDGCHC 3 cut(s) 214, 261, 595
SexAI ACCWGGT 1 cut(s) 867
SfaNI GCATC 2 cut(s) 1162, 1332
SfcI CTRYAG 2 cut(s) 147, 1167
SgrDI CGTCGACG 1 cut(s) 179
SinI GGWCC 2 cut(s) 9, 542
SmiMI CAYNNNNRTG 2 cut(s) 839, 894
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 7 cut(s) 350, 460, 731, 755, 825, 969, 1320
SsiI CCGC 5 cut(s) 7, 153, 185, 597, 1345
SspI AATATT 2 cut(s) 712, 771
SspMI CTAG 1 cut(s) 428
StyD4I CCNGG 1 cut(s) 867
StyI CCWWGG 3 cut(s) 44, 90, 834
TaaI ACNGT 4 cut(s) 109, 542, 1024, 1066
TaiI ACGT 2 cut(s) 180, 998
TaqI TCGA 7 cut(s) 19, 104, 180, 195, 249, 466, 1036
TasI AATT 7 cut(s) 350, 460, 731, 755, 825, 969, 1320
TauI GCSGC 2 cut(s) 155, 188
TfiI GAWTC 2 cut(s) 740, 882
Tru1I TTAA 2 cut(s) 735, 749
Tru9I TTAA 2 cut(s) 735, 749
TscAI CASTG 2 cut(s) 286, 1006
TseI GCWGC 4 cut(s) 149, 899, 1211, 1214
TspDTI ATGAA 5 cut(s) 246, 753, 830, 1023, 1221
TspGWI ACGGA 2 cut(s) 231, 1283
TspRI CASTG 2 cut(s) 286, 1006
VpaK11BI GGWCC 2 cut(s) 9, 542
XapI RAATTY 1 cut(s) 731
XceI RCATGY 1 cut(s) 1049
XmiI GTMKAC 1 cut(s) 180
XmnI GAANNNNTTC 2 cut(s) 711, 722
XspI CTAG 1 cut(s) 428
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.