MD03G1095000.v1.1

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0020367
Physical Location & Seq
Forward (+)
8057427 .. 8057789
363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1095000.v1.1.491

Sequence Viewer

Length: 363 bp
ATGTTACAAGATTATTTGCGTATTGATATCCGGTATTTCCCTGTCTGCGTGCAGCCTAAATGGAACAATGCACCTCTAGAGGCGTTCCAAATCGACTACATAGGGACTTCCCCATACATCACTTGTTCGCCATCTCTCCACTACCACAGATTAGGTCCCAAAGACAGGTTTTTGATATTATCCTCCGATGGATTGTATCAATACTTTACCAATGAAGAAGCTGTTTCTGAAGTTGAACTTTTCATCACATTGCAACCTGAAGGAGATCCGGCGCAGCATCCGGTTGATGAAGTGCTGTTTCATGCTGCAAAAAAGGCCGGAAAGTTGCGGTCAAAATTAGAGAAAACATTGTTCTTAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0016020 GO:0032501 GO:0032502 GO:0044464 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0071944 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

121

Amino Acids

13.86

Weight (kDa)

5.75

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 37 - 84 4.1e-08 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020367)

Species Orthologous Gene IDs
malus_domestica MD03G1095000.v1.1
prunus_persica Prupe.5G040800_v2.0.a1
rosa_multiflora Rmu_co8174702.1_g000001 Rmu_sc0009521.1_g000002
rosa_samantha Rh6AG120300 Rh6BG113600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 328
AclWI GGATC 1 cut(s) 260
AcuI CTGAAG 2 cut(s) 249, 279
AfiI CCNNNNNNNGG 1 cut(s) 165
AgsI TTSAA 1 cut(s) 236
AluBI AGCT 1 cut(s) 221
AluI AGCT 1 cut(s) 221
AlwI GGATC 1 cut(s) 260
AoxI GGCC 1 cut(s) 315
ApeKI GCWGC 3 cut(s) 52, 274, 305
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 1 cut(s) 155
AvaII GGWCC 1 cut(s) 155
BbvI GCAGC 3 cut(s) 64, 286, 292
BccI CCATC 2 cut(s) 139, 182
BfaI CTAG 2 cut(s) 77, 361
BisI GCNGC 3 cut(s) 53, 275, 306
BlsI GCNGC 3 cut(s) 54, 276, 307
Bme18I GGWCC 1 cut(s) 155
BmgT120I GGNCC 1 cut(s) 155
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 1 cut(s) 286
BsaWI WCCGGW 2 cut(s) 30, 280
Bsc4I CCNNNNNNNGG 1 cut(s) 165
Bse3DI GCAATG 1 cut(s) 248
BseGI GGATG 1 cut(s) 277
BseLI CCNNNNNNNGG 1 cut(s) 165
BseMI GCAATG 1 cut(s) 248
BseXI GCAGC 3 cut(s) 64, 286, 292
BsgI GTGCAG 1 cut(s) 71
BshFI GGCC 1 cut(s) 317
BsiSI CCGG 4 cut(s) 31, 269, 281, 318
BslFI GGGAC 2 cut(s) 118, 141
BslI CCNNNNNNNGG 1 cut(s) 165
BsmFI GGGAC 2 cut(s) 118, 141
BsnI GGCC 1 cut(s) 317
Bsp143I GATC 1 cut(s) 265
BspACI CCGC 1 cut(s) 328
BspANI GGCC 1 cut(s) 317
BspLI GGNNCC 1 cut(s) 157
BspPI GGATC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 248
BssMI GATC 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 50
BstF5I GGATG 1 cut(s) 277
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 1 cut(s) 268
BstMBI GATC 1 cut(s) 265
BstMWI GCNNNNNNNGC 1 cut(s) 314
BstV1I GCAGC 3 cut(s) 64, 286, 292
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 1 cut(s) 317
BtsCI GGATG 1 cut(s) 277
Cac8I GCNNGC 1 cut(s) 50
CfoI GCGC 1 cut(s) 274
Cfr13I GGNCC 1 cut(s) 155
CviAII CATG 1 cut(s) 302
CviJI RGCY 3 cut(s) 55, 221, 317
CviKI_1 RGCY 3 cut(s) 55, 221, 317
DpnI GATC 1 cut(s) 267
DpnII GATC 1 cut(s) 265
Eco32I GATATC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 155
Eco57I CTGAAG 2 cut(s) 249, 279
EcoO109I RGGNCCY 1 cut(s) 155
EcoRV GATATC 1 cut(s) 28
FaeI CATG 1 cut(s) 305
FaiI YATR 3 cut(s) 101, 115, 303
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FaqI GGGAC 2 cut(s) 118, 141
FatI CATG 1 cut(s) 301
Fnu4HI GCNGC 3 cut(s) 53, 275, 306
FokI GGATG 1 cut(s) 264
Fsp4HI GCNGC 3 cut(s) 53, 275, 306
FspBI CTAG 2 cut(s) 77, 361
GlaI GCGC 1 cut(s) 273
GluI GCNGC 3 cut(s) 53, 275, 306
HaeIII GGCC 1 cut(s) 317
HapII CCGG 4 cut(s) 31, 269, 281, 318
HhaI GCGC 1 cut(s) 274
Hin1II CATG 1 cut(s) 305
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HpaII CCGG 4 cut(s) 31, 269, 281, 318
Hpy188I TCNGA 2 cut(s) 187, 229
Hpy188III TCNNGA 1 cut(s) 77
HpyAV CCTTC 1 cut(s) 254
HpyCH4V TGCA 4 cut(s) 52, 71, 253, 308
HpyF10VI GCNNNNNNNGC 1 cut(s) 314
Hsp92II CATG 1 cut(s) 305
HspAI GCGC 1 cut(s) 272
Kzo9I GATC 1 cut(s) 265
LpnPI CCDG 7 cut(s) 44, 54, 151, 270, 282, 294, 331
Lsp1109I GCAGC 3 cut(s) 64, 286, 292
LweI GCATC 1 cut(s) 286
MaeI CTAG 2 cut(s) 77, 361
MaeIII GTNAC 1 cut(s) 3
MalI GATC 1 cut(s) 267
MboI GATC 1 cut(s) 265
MboII GAAGA 1 cut(s) 227
MflI RGATCY 1 cut(s) 265
MluCI AATT 1 cut(s) 335
MnlI CCTC 3 cut(s) 73, 84, 193
MseI TTAA 1 cut(s) 356
MspI CCGG 4 cut(s) 31, 269, 281, 318
MwoI GCNNNNNNNGC 1 cut(s) 314
NdeII GATC 1 cut(s) 265
NlaIII CATG 1 cut(s) 305
NlaIV GGNNCC 1 cut(s) 157
PkrI GCNGC 3 cut(s) 54, 276, 307
PpuMI RGGWCCY 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 155
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 1 cut(s) 155
PspPPI RGGWCCY 1 cut(s) 155
PsuI RGATCY 1 cut(s) 265
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 3 cut(s) 53, 275, 306
Sau3AI GATC 1 cut(s) 265
Sau96I GGNCC 1 cut(s) 155
SetI ASST 5 cut(s) 76, 157, 170, 223, 259
SfaNI GCATC 1 cut(s) 286
SinI GGWCC 1 cut(s) 155
Sse9I AATT 1 cut(s) 335
SsiI CCGC 1 cut(s) 328
SspMI CTAG 2 cut(s) 77, 361
TaqI TCGA 1 cut(s) 93
TasI AATT 1 cut(s) 335
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TseI GCWGC 3 cut(s) 52, 274, 305
TspDTI ATGAA 4 cut(s) 228, 232, 290, 303
VpaK11BI GGWCC 1 cut(s) 155
XbaI TCTAGA 1 cut(s) 76
XspI CTAG 2 cut(s) 77, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.