Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010178

Physical Location & Seq

Genomic Coordinates Forward (+)

8171283 .. 8177186

Gene Structure 5904 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1096300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2016 bp

ATGGCCGAGATCGGTGGCGACCCAGCTATGGAGGAAGACAGGGGTGCTCTGGTGCAGAGCCTGATCGACACGATCAACGAAATAGCTTCGATCTCCGACTACAGGTGTGTGGTCAAGAAGCAGTACTGCAATTTGGCGAGGAGGTTGAAGCTGCTGACCCCAATGTTCGAGGAGATTAGGGATAGCAAGGAGGAGGTCTCTCAGGACACTCTGAAGGCCTTGCTTTCGTTCATGGAAGCTCTGGAATCGGCCAAGGAGCTGCTCAGATTCGGAGGCGAGAGCAGCAAGATTTACCTGGTTTTGGAAAGGGAGCAAATTATGATGAAGTTTCATGAGGTGACAGCTCGATTGGAGCAAGCTTTAAGTGGACTTTCCTATGAAAATCTTGACATATCTGATGAAGTTAAGGAACAGGTTGAGCTTGTTCTTGCTCAGTTTAGAAGAGCCAAAGGAAGGTTCGATTCGCCTGATGTTGAGCTCTATGAAGATCTCTTGTCCATTTACTGTAAAAATGAGGCAGCAACAGATCCAACTGTCCTAAGAAGATTGGTCGATAAGTTAGAATTGACAGGGATAGCTGAACTGACACAAGAATCACTAGCCTTGCATGAGATGGTTACTTTCAGTGGTGGGGATCCTGGGGAGAGCATCGAGAAGATGTCAATGCTACTGAAAAAAATTAAGGATTTTGTACAAACAGAAAACCCCGACATGGATGCTCCTGCAGCAGAAAATAATATATTAGCAAGTTGCAGTGGGCAAGCATCCACTGATAAGAATCACAAAGTCCCCCCAGTTATACCAGATGATTTTCGTTGTCCAATATCTTTGGAGTTAATGAAGGATCCCGTCATTGTTTCAACAGGGCAGACATATGATCGTTCCTGTATTGAGAGGTGGCTGGAAGCAGGTCATGGGACATGTCCAAAAACACAACAGAGCCTCTCTAACACCCTCACTCCGAACTATGCTTTACGTAGCCTCATTGCCCAGTGGTGCGAGGCAAATGGCATTGAACCACCAAAAAGACCCAATTCTCGACCTAATAAAACTGCATCCGCCTGCTCGCCAGCAGAACACACTAAGATTGAAATTCTCCTCCACAAGCTGATGTCAGTAAACCCCGAAGACCAAAGGTCTGCCGCTGGTGAAATCCGCCTTCTTGCCAAACGTAATGCAGATAATCGTGTGGCTATTGCTGAAGCTGGTGCAATACCCCTGCTTGTAGGCCTCCTTTCAACCCCTGACTCCCGCACCCAAGAGCATGCTGTGACAGCACTCCTCAACCTTTCCATATGTGAGGATAACAAAGGAAGCATCATCTCATCTGGAGCAATTCCTGGTATTGTTCATGTCCTCAAGAATGGAGGGATGGAAGCACGGGAAAATGCAGCTGCGACCCTTTTCAGCCTTTCTGTTGTGGATGAAAATAAGGTTAGAATTGGTGCTTCAGGAGCTATTCCGCCACTTGTTAAATTGCTGAGTGAAGGTACCCAAAGGGGGAAGAAAGATGCTGCAACTGCACTTTTTAACTTGTGCATTTATCAAGGTAACAAGGGGAAGGCAGTAAGGGCCGGTGTTGTTTCGACCCTAATGAAGCTGCTGACAGAACCTGGAGGTGGAATGGTGGATGAAGCACTTGCCATTCTAGCAATATTGTCTAGCCACCCTGAAGGGAAAGCGGCCATTGGAGCTGCTGAGGCTGTGCCTGTTTTGGTTGAAGTTATTGGGACTGGATCTCCCCGAAACAGAGAAAATGCAGCTGCAGTTTTGGTGCATCTTTGTTCTGGAGACCAACAACATATAATAGAGGCTCAGAAACTCGGGGTGATGAGTTTGTTATTGGAGTTGGCACAAAATGGCACAGATAGAGGCAAGAGAAAGGCTGCGCAGTTACTTGAGCGAGTGAATCGGTTTGCTGAGCAGCAGATGCAGGCGCAGGCCCAGGCACAAGCCGAGGCTCAGCCTGAGACCCAGTCACATCCACCCACTACTACAAATGCCATTGACAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0003824 GO:0004842 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0015934 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019843 GO:0022625 GO:0022626 GO:0031347 GO:0031348 GO:0032446 GO:0032991 GO:0033612 GO:0036211 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048586 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0060548 GO:0065007 GO:0070647 GO:0070696 GO:0071704 GO:0080134 GO:0097159 GO:0098542 GO:0140096 GO:1901363 GO:1901564 GO:1990904 GO:2000026 GO:2000028 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

672

Amino Acids

72.86

Weight (kDa)

5.34

Isoelectric Point (pI)

41.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB2_N	PF25240	18 - 124	1e-06	PUB2-like, N-terminal domain
MLKL_N	PF22215	38 - 125	9.6e-08	Mixed lineage kinase domain-like N-terminal domain
PUB10_N	PF25368	39 - 150	1.3e-38	PUB10-like, N-terminal domain
U-box	PF04564	267 - 336	1.1e-20	U-box domain
ARM_PUB	PF25598	363 - 639	1e-27	Plant U-box protein ARM repeat region
ARM_ARMC5	PF24768	374 - 537	8.2e-10	ARMC5
Arm	PF00514	392 - 431	2.4e-10	Armadillo/beta-catenin-like repeat
ARM_ARMC5	PF24768	427 - 621	9.8e-11	ARMC5
Arm	PF00514	475 - 513	2.5e-07	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010178)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46510
fragaria_vesca	FvH4_3g35800 FvH4_3g35800 FvH4_3g35800
malus_domestica	MD03G1096300.v1.1 MD11G1111000.v1.1
prunus_persica	Prupe.6G079400_v2.0.a1 Prupe.6G079400_v2.0.a1 Prupe.6G079400_v2.0.a1
pyrus_communis	pycom03g07890 pycom11g09180
rosa_chinensis	RchiOBHm_Chr5g0063991
rosa_laevigata	RLG00000035696
rosa_multiflora	Rmu_sc0008881.1_g000007
rosa_roxburghii	Rroxscaffold_1G00016810
rosa_rugosa	Rorug05G0361600 Rorug05G0361700
rosa_samantha	Rh5BG435800 Rh5CG458900 Rh5DG449000
rosa_wichuraiana	Rw5G039470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1890
Acc36I	ACCTGC	1 cut(s)	899
Acc65I	GGTACC	1 cut(s)	1490
AccB1I	GGYRCC	1 cut(s)	1490
AciI	CCGC	6 cut(s)	1059, 1143, 1156, 1252, 1463, 1682
AclWI	GGATC	6 cut(s)	521, 629, 642, 839, 852, 1744
AcoI	YGGCCR	3 cut(s)	3, 249, 1683
AcsI	RAATTY	1 cut(s)	1092
AcuI	CTGAAG	4 cut(s)	233, 1221, 1434, 1692
AfaI	GTAC	3 cut(s)	125, 693, 1492
AfiI	CCNNNNNNNGG	7 cut(s)	28, 102, 301, 453, 712, 1500, 1619
AflIII	ACRYGT	1 cut(s)	920
AgsI	TTSAA	6 cut(s)	148, 861, 1016, 1091, 1239, 1721
AhdI	GACNNNNNGTC	1 cut(s)	1135
AjnI	CCWGG	5 cut(s)	294, 637, 1339, 1612, 1944
AjuI	GAANNNNNNNTTGG	2 cut(s)	440, 472
Alw21I	GWGCWC	2 cut(s)	49, 480
Alw26I	GTCTC	3 cut(s)	202, 1785, 1964
AlwI	GGATC	6 cut(s)	521, 629, 642, 839, 852, 1744
AlwNI	CAGNNNCTG	2 cut(s)	61, 1613
Ama87I	CYCGRG	1 cut(s)	1823
AoxI	GGCC	7 cut(s)	3, 216, 249, 1228, 1572, 1683, 1941
ApoI	RAATTY	1 cut(s)	1092
Asp718I	GGTACC	1 cut(s)	1490
AspLEI	GCGC	2 cut(s)	1891, 1939
AspS9I	GGNCC	2 cut(s)	1572, 1942
AsuHPI	GGTGA	3 cut(s)	349, 1160, 1840
AvaI	CYCGRG	1 cut(s)	1823
BamHI	GGATCC	2 cut(s)	634, 844
BanI	GGYRCC	1 cut(s)	1490
BanII	GRGCYC	1 cut(s)	480
BbsI	GAAGAC	2 cut(s)	42, 1134
Bbv12I	GWGCWC	2 cut(s)	49, 480
BbvCI	CCTCAGC	1 cut(s)	1698
BccI	CCATC	2 cut(s)	607, 1366
BcgI	CGANNNNNNTGC	2 cut(s)	698, 732
BciT130I	CCWGG	5 cut(s)	296, 639, 1341, 1614, 1946
BcoDI	GTCTC	3 cut(s)	202, 1785, 1964
BfaI	CTAG	3 cut(s)	599, 1649, 1662
BfmI	CTRYAG	3 cut(s)	100, 723, 1764
BfuAI	ACCTGC	1 cut(s)	899
BglII	AGATCT	1 cut(s)	487
BlpI	GCTNAGC	2 cut(s)	1920, 1962
BmcAI	AGTACT	1 cut(s)	125
Bme1390I	CCNGG	5 cut(s)	296, 639, 1341, 1614, 1946
BmeRI	GACNNNNNGTC	1 cut(s)	1135
BmeT110I	CYCGRG	1 cut(s)	1823
BmgT120I	GGNCC	2 cut(s)	1572, 1942
BmiI	GGNNCC	3 cut(s)	636, 846, 1492
BmrFI	CCNGG	5 cut(s)	296, 639, 1341, 1614, 1946
BmrI	ACTGGG	3 cut(s)	788, 985, 1969
BmsI	GCATC	8 cut(s)	657, 706, 773, 1064, 1326, 1501, 1786, 1920
BmuI	ACTGGG	3 cut(s)	788, 985, 1969
BpiI	GAAGAC	2 cut(s)	42, 1134
BplI	GAGNNNNNCTC	2 cut(s)	182, 214
BpmI	CTGGAG	3 cut(s)	1350, 1635, 1809
Bpu10I	CCTNAGC	1 cut(s)	1698
Bpu1102I	GCTNAGC	2 cut(s)	1920, 1962
BpuEI	CTTGAG	2 cut(s)	1343, 1919
BsaAI	YACGTR	1 cut(s)	977
BsaBI	GATNNNNATC	1 cut(s)	777
BsaI	GGTCTC	3 cut(s)	202, 1785, 1964
BsaJI	CCNNGG	4 cut(s)	252, 638, 1944, 1956
BsaXI	ACNNNNNCTCC	4 cut(s)	943, 973, 1723, 1753
Bsc4I	CCNNNNNNNGG	7 cut(s)	28, 102, 301, 453, 712, 1500, 1619
Bse118I	RCCGGY	1 cut(s)	1574
Bse1I	ACTGG	4 cut(s)	794, 991, 1738, 1975
Bse3DI	GCAATG	1 cut(s)	984
Bse8I	GATNNNNATC	1 cut(s)	777
BseBI	CCWGG	5 cut(s)	296, 639, 1341, 1614, 1946
BseDI	CCNNGG	4 cut(s)	252, 638, 1944, 1956
BseGI	GGATG	7 cut(s)	721, 764, 1055, 1377, 1429, 1636, 1981
BseJI	GATNNNNATC	1 cut(s)	777
BseLI	CCNNNNNNNGG	7 cut(s)	28, 102, 301, 453, 712, 1500, 1619
BseMI	GCAATG	1 cut(s)	984
BseMII	CTCAG	9 cut(s)	215, 277, 446, 1472, 1689, 1829, 1911, 1959, 1976
BseNI	ACTGG	4 cut(s)	794, 991, 1738, 1975
BseRI	GAGGAG	5 cut(s)	154, 185, 206, 1088, 1271
BseYI	CCCAGC	1 cut(s)	22
BsgI	GTGCAG	2 cut(s)	74, 1506
BshFI	GGCC	7 cut(s)	5, 218, 251, 1230, 1574, 1685, 1943
BshNI	GGYRCC	1 cut(s)	1490
BsiHKAI	GWGCWC	2 cut(s)	49, 480
BsiHKCI	CYCGRG	1 cut(s)	1823
BsiSI	CCGG	1 cut(s)	1575
BslFI	GGGAC	3 cut(s)	773, 931, 1744
BslI	CCNNNNNNNGG	7 cut(s)	28, 102, 301, 453, 712, 1500, 1619
BsmAI	GTCTC	3 cut(s)	202, 1785, 1964
BsmFI	GGGAC	3 cut(s)	773, 931, 1744
BsnI	GGCC	7 cut(s)	5, 218, 251, 1230, 1574, 1685, 1943
Bso31I	GGTCTC	3 cut(s)	202, 1785, 1964
BsoBI	CYCGRG	1 cut(s)	1823
Bsp1286I	GDGCHC	2 cut(s)	49, 480
Bsp1407I	TGTACA	1 cut(s)	691
Bsp1720I	GCTNAGC	2 cut(s)	1920, 1962
BspACI	CCGC	6 cut(s)	1059, 1143, 1156, 1252, 1463, 1682
BspANI	GGCC	7 cut(s)	5, 218, 251, 1230, 1574, 1685, 1943
BspCNI	CTCAG	9 cut(s)	214, 276, 445, 1473, 1690, 1828, 1912, 1960, 1975
BspHI	TCATGA	1 cut(s)	331
BspLI	GGNNCC	3 cut(s)	636, 846, 1492
BspMAI	CTGCAG	2 cut(s)	727, 1768
BspMI	ACCTGC	1 cut(s)	899
BspPI	GGATC	6 cut(s)	521, 629, 642, 839, 852, 1744
BspQI	GCTCTTC	1 cut(s)	436
BspT107I	GGYRCC	1 cut(s)	1490
BspTNI	GGTCTC	3 cut(s)	202, 1785, 1964
BsrDI	GCAATG	1 cut(s)	984
BsrFI	RCCGGY	1 cut(s)	1574
BsrGI	TGTACA	1 cut(s)	691
BsrI	ACTGG	4 cut(s)	794, 991, 1738, 1975
BssAI	RCCGGY	1 cut(s)	1574
BssECI	CCNNGG	4 cut(s)	252, 638, 1944, 1956
BssT1I	CCWWGG	1 cut(s)	252
Bst2UI	CCWGG	5 cut(s)	296, 639, 1341, 1614, 1946
Bst4CI	ACNGT	2 cut(s)	506, 535
Bst6I	CTCTTC	1 cut(s)	436
BstAPI	GCANNNNNTGC	1 cut(s)	1930
BstAUI	TGTACA	1 cut(s)	691
BstBAI	YACGTR	1 cut(s)	977
BstC8I	GCNNGC	8 cut(s)	357, 762, 1063, 1067, 1071, 1266, 1935, 1941
BstF5I	GGATG	7 cut(s)	721, 764, 1055, 1377, 1429, 1636, 1981
BstHHI	GCGC	2 cut(s)	1891, 1939
BstMAI	GTCTC	3 cut(s)	202, 1785, 1964
BstNI	CCWGG	5 cut(s)	296, 639, 1341, 1614, 1946
BstNSI	RCATGY	2 cut(s)	924, 1268
BstSCI	CCNGG	5 cut(s)	294, 637, 1339, 1612, 1944
BstSFI	CTRYAG	3 cut(s)	100, 723, 1764
BstSNI	TACGTA	1 cut(s)	977
BstV2I	GAAGAC	2 cut(s)	42, 1134
BstX2I	RGATCY	5 cut(s)	487, 526, 634, 844, 1736
BstYI	RGATCY	5 cut(s)	487, 526, 634, 844, 1736
BsuRI	GGCC	7 cut(s)	5, 218, 251, 1230, 1574, 1685, 1943
BtsCI	GGATG	7 cut(s)	721, 764, 1055, 1377, 1429, 1636, 1981
BtsI	GCAGTG	1 cut(s)	760
BtsIMutI	CAGTG	4 cut(s)	631, 760, 768, 998
BveI	ACCTGC	1 cut(s)	899
Cac8I	GCNNGC	8 cut(s)	357, 762, 1063, 1067, 1071, 1266, 1935, 1941
CaiI	CAGNNNCTG	2 cut(s)	61, 1613
CciI	TCATGA	1 cut(s)	331
CfoI	GCGC	2 cut(s)	1891, 1939
Cfr10I	RCCGGY	1 cut(s)	1574
Cfr13I	GGNCC	2 cut(s)	1572, 1942
CsiI	ACCWGGT	1 cut(s)	294
Csp6I	GTAC	3 cut(s)	124, 692, 1491
CviAII	CATG	8 cut(s)	232, 332, 608, 712, 914, 921, 1265, 1352
CviQI	GTAC	3 cut(s)	124, 692, 1491
DriI	GACNNNNNGTC	1 cut(s)	1135
EaeI	YGGCCR	3 cut(s)	3, 249, 1683
Eam1104I	CTCTTC	1 cut(s)	436
Eam1105I	GACNNNNNGTC	1 cut(s)	1135
EarI	CTCTTC	1 cut(s)	436
EciI	GGCGGA	3 cut(s)	1048, 1145, 1452
Ecl136II	GAGCTC	1 cut(s)	478
Eco105I	TACGTA	1 cut(s)	977
Eco130I	CCWWGG	1 cut(s)	252
Eco147I	AGGCCT	2 cut(s)	218, 1230
Eco24I	GRGCYC	1 cut(s)	480
Eco31I	GGTCTC	3 cut(s)	202, 1785, 1964
Eco53kI	GAGCTC	1 cut(s)	478
Eco57I	CTGAAG	4 cut(s)	233, 1221, 1434, 1692
Eco88I	CYCGRG	1 cut(s)	1823
EcoICRI	GAGCTC	1 cut(s)	478
EcoRII	CCWGG	5 cut(s)	294, 637, 1339, 1612, 1944
EcoT14I	CCWWGG	1 cut(s)	252
EcoT38I	GRGCYC	1 cut(s)	480
ErhI	CCWWGG	1 cut(s)	252
FaeI	CATG	8 cut(s)	235, 335, 611, 715, 917, 924, 1268, 1355
FaqI	GGGAC	3 cut(s)	773, 931, 1744
FatI	CATG	8 cut(s)	231, 331, 607, 711, 913, 920, 1264, 1351
FauI	CCCGC	1 cut(s)	1259
FauNDI	CATATG	2 cut(s)	874, 1295
FokI	GGATG	7 cut(s)	728, 751, 1042, 1384, 1436, 1643, 1968
FriOI	GRGCYC	1 cut(s)	480
FspBI	CTAG	3 cut(s)	599, 1649, 1662
FspI	TGCGCA	1 cut(s)	1890
GlaI	GCGC	2 cut(s)	1890, 1938
GsaI	CCCAGC	1 cut(s)	26
GsuI	CTGGAG	3 cut(s)	1350, 1635, 1809
HaeIII	GGCC	7 cut(s)	5, 218, 251, 1230, 1574, 1685, 1943
HapII	CCGG	1 cut(s)	1575
HhaI	GCGC	2 cut(s)	1891, 1939
Hin1II	CATG	8 cut(s)	235, 335, 611, 715, 917, 924, 1268, 1355
Hin6I	GCGC	2 cut(s)	1889, 1937
HinP1I	GCGC	2 cut(s)	1889, 1937
HindIII	AAGCTT	1 cut(s)	357
HinfI	GANTC	7 cut(s)	245, 267, 461, 593, 778, 1247, 1909
HpaII	CCGG	1 cut(s)	1575
HphI	GGTGA	3 cut(s)	349, 1160, 1840
Hpy166II	GTNNAC	2 cut(s)	368, 1120
Hpy188I	TCNGA	7 cut(s)	97, 213, 266, 272, 397, 963, 1818
Hpy8I	GTNNAC	2 cut(s)	368, 1120
HpyAV	CCTTC	7 cut(s)	208, 447, 835, 1169, 1481, 1555, 1667
HpyCH4III	ACNGT	2 cut(s)	506, 535
HpyCH4IV	ACGT	2 cut(s)	976, 1171
HpySE526I	ACGT	2 cut(s)	976, 1171
Hsp92II	CATG	8 cut(s)	235, 335, 611, 715, 917, 924, 1268, 1355
HspAI	GCGC	2 cut(s)	1889, 1937
KpnI	GGTACC	1 cut(s)	1494
LguI	GCTCTTC	1 cut(s)	436
LmnI	GCTCC	7 cut(s)	256, 310, 352, 724, 1331, 1454, 1691
LweI	GCATC	8 cut(s)	657, 706, 773, 1064, 1326, 1501, 1786, 1920
MabI	ACCWGGT	1 cut(s)	294
MaeI	CTAG	3 cut(s)	599, 1649, 1662
MaeII	ACGT	2 cut(s)	976, 1171
MaeIII	GTNAC	6 cut(s)	337, 616, 1270, 1550, 1893, 1977
MboII	GAAGA	7 cut(s)	47, 453, 497, 555, 667, 1139, 1516
MflI	RGATCY	5 cut(s)	487, 526, 634, 844, 1736
MhlI	GDGCHC	2 cut(s)	49, 480
MluCI	AATT	9 cut(s)	130, 315, 563, 678, 1033, 1092, 1335, 1440, 1475
MlyI	GAGTC	1 cut(s)	1241
MmeI	TCCRAC	2 cut(s)	120, 554
MseI	TTAA	6 cut(s)	362, 405, 681, 836, 1473, 1530
MspA1I	CMGCKG	3 cut(s)	1145, 1394, 1763
MspI	CCGG	1 cut(s)	1575
MspR9I	CCNGG	5 cut(s)	296, 639, 1341, 1614, 1946
MvaI	CCWGG	5 cut(s)	296, 639, 1341, 1614, 1946
NdeI	CATATG	2 cut(s)	874, 1295
NlaIII	CATG	8 cut(s)	235, 335, 611, 715, 917, 924, 1268, 1355
NlaIV	GGNNCC	3 cut(s)	636, 846, 1492
NmeAIII	GCCGAG	2 cut(s)	31, 1981
NmuCI	GTSAC	3 cut(s)	337, 1270, 1977
NsbI	TGCGCA	1 cut(s)	1890
NspI	RCATGY	2 cut(s)	924, 1268
PaeI	GCATGC	1 cut(s)	1268
PagI	TCATGA	1 cut(s)	331
PceI	AGGCCT	2 cut(s)	218, 1230
PciI	ACATGT	1 cut(s)	920
PciSI	GCTCTTC	1 cut(s)	436
PfeI	GAWTC	6 cut(s)	245, 267, 461, 593, 778, 1909
PflFI	GACNNNGTC	1 cut(s)	1975
PleI	GAGTC	1 cut(s)	1241
PpsI	GAGTC	1 cut(s)	1241
Ppu21I	YACGTR	1 cut(s)	977
PscI	ACATGT	1 cut(s)	920
Psp124BI	GAGCTC	1 cut(s)	480
Psp6I	CCWGG	5 cut(s)	294, 637, 1339, 1612, 1944
PspFI	CCCAGC	1 cut(s)	22
PspGI	CCWGG	5 cut(s)	294, 637, 1339, 1612, 1944
PspN4I	GGNNCC	3 cut(s)	636, 846, 1492
PspPI	GGNCC	2 cut(s)	1572, 1942
PstI	CTGCAG	2 cut(s)	727, 1768
PstNI	CAGNNNCTG	2 cut(s)	61, 1613
PsuI	RGATCY	5 cut(s)	487, 526, 634, 844, 1736
PsyI	GACNNNGTC	1 cut(s)	1975
PvuII	CAGCTG	2 cut(s)	1394, 1763
RsaI	GTAC	3 cut(s)	125, 693, 1492
RsaNI	GTAC	3 cut(s)	124, 692, 1491
SacI	GAGCTC	1 cut(s)	480
SapI	GCTCTTC	1 cut(s)	436
SaqAI	TTAA	6 cut(s)	362, 405, 681, 836, 1473, 1530
Sau96I	GGNCC	2 cut(s)	1572, 1942
ScaI	AGTACT	1 cut(s)	125
SchI	GAGTC	1 cut(s)	1241
ScrFI	CCNGG	5 cut(s)	296, 639, 1341, 1614, 1946
SduI	GDGCHC	2 cut(s)	49, 480
SexAI	ACCWGGT	1 cut(s)	294
SfaNI	GCATC	8 cut(s)	657, 706, 773, 1064, 1326, 1501, 1786, 1920
SfcI	CTRYAG	3 cut(s)	100, 723, 1764
SmlI	CTYRAG	2 cut(s)	1358, 1898
SmoI	CTYRAG	2 cut(s)	1358, 1898
SnaBI	TACGTA	1 cut(s)	977
SphI	GCATGC	1 cut(s)	1268
Sse9I	AATT	9 cut(s)	130, 315, 563, 678, 1033, 1092, 1335, 1440, 1475
SseBI	AGGCCT	2 cut(s)	218, 1230
SsiI	CCGC	6 cut(s)	1059, 1143, 1156, 1252, 1463, 1682
SspI	AATATT	1 cut(s)	1656
SspMI	CTAG	3 cut(s)	599, 1649, 1662
SstI	GAGCTC	1 cut(s)	480
StuI	AGGCCT	2 cut(s)	218, 1230
StyD4I	CCNGG	5 cut(s)	294, 637, 1339, 1612, 1944
StyI	CCWWGG	1 cut(s)	252
TaaI	ACNGT	2 cut(s)	506, 535
TaiI	ACGT	2 cut(s)	979, 1174
TaqI	TCGA	9 cut(s)	66, 89, 168, 346, 459, 552, 651, 1039, 1586
TasI	AATT	9 cut(s)	130, 315, 563, 678, 1033, 1092, 1335, 1440, 1475
TatI	WGTACW	2 cut(s)	123, 691
TauI	GCSGC	2 cut(s)	1145, 1685
TfiI	GAWTC	6 cut(s)	245, 267, 461, 593, 778, 1909
Tru1I	TTAA	6 cut(s)	362, 405, 681, 836, 1473, 1530
Tru9I	TTAA	6 cut(s)	362, 405, 681, 836, 1473, 1530
TscAI	CASTG	4 cut(s)	631, 760, 775, 998
TseFI	GTSAC	3 cut(s)	337, 1270, 1977
Tsp45I	GTSAC	3 cut(s)	337, 1270, 1977
TspRI	CASTG	4 cut(s)	631, 760, 775, 998
Tth111I	GACNNNGTC	1 cut(s)	1975
XapI	RAATTY	1 cut(s)	1092
XceI	RCATGY	2 cut(s)	924, 1268
XspI	CTAG	3 cut(s)	599, 1649, 1662
ZrmI	AGTACT	1 cut(s)	125

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.