Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0010178

Physical Location & Seq

Genomic Coordinates Forward (+)

20889567 .. 20895030

Gene Structure 5464 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00016810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGGAGGAAGATAAAGGTGCTCTGGTGCAGAACCTGATTGAGACAGTCAACGAAATAGCTTCGATCTCCGACTACAGATGCACGGTGAAGAAGCAGTACTGTAATTTGGCCCGGAGGTTGAAGCTATTGACTCCAATGTTCGAGGAGATTAGGGATTTGAAAGAGGCGGTGCTGCCTGAGGACACTGTGAAAGCTTTGGTTTCGTTCAGGGAAGCGCTGGAATCGGCTAAGGAGTTGCTCAGATTCGGCAGCGAAAGCAGCAAGATTTACCTGGTCCTGGAAAGAGAGCAAACTATGAATAAGTTCCATGAGGTGACAGCTCGATTGGAACAAACTTTAAGTGGAATTTCCTACGAAGAGCTTGATATATCAGATGAAGTGAAGGAACAGGTTGAGCTTGTGCTTGCTCAGTTTAGAAGGGCCAAAGGAAGAGTTGATTTACCCGATGTTGAGCTATATGAGGATCTCTCATACCTTTACAATAGAAATGATGCAGCAACAGATCCAGCAGTCTTTAGACGACTGGTTGAAAAGTTACAATTGGCAGGGATAGGTGAACTTACACAAGAGTCACTTGCATTGCATGAGATGGTTGCCTCTAGTGGTGGGGATCCTGGAGCAAGCATTGAGAAAATGTCAATGCTATTGAAAAAGATTAAGGATTTTGTACAAACAGAAAATCCCAACATGGATGCTCCTGCTACAGAAAAGAGTGTCGTTGCAACTTCCAGTGGACAAGCATCCACTGACAGGAATCGCAAGGCCCCAGTTATACCTGATGATTTTCGATGTCCAATATCATTGGAATTGATGAAGGATCCAGTCATTGTTTCAACGGGGCAGACATATGAACGTTCCTGTATTGAGAAGTGGCTGGAACAAGGGCATGTTACATGTCCGAAAACACAACAGACCCTCTCTAGCACCAGTCTCACACCGAACTATGTTTTACGTAGCCTTATAGCCCAGTGGTGTGAGGCAAATGGTATTGAAGTCCCAAAAAGGCCCAATCGACCCAATAAAACTGCATCTGCTTGCTCGCCCGCGGAACGTACTAAGATTGAAAATCTTCTTCACAAACTAACATCAGCAAACCCTGAAGACCAAAGGTCTGCTGCTGGTGAAATCCGCCTTCTTGCCAAGCGTAATGCAGATAATCGTGTGGCAATTGCTGAAGCTGGTGCAATCCCCCTACTTGTAGGGCTGCTTTCAACCCCTGATTCCCGCACCCAAGAGCATGCTGTGACGGCGCTTCTCAACCTTTCAATATGTGAGGATAACAAAGGAAGCATCATTTCCTGTGGAGCAGTTCCTGGTATTGTTCATGTGCTCAAGCACGGTGGCATGGAAGCACGGGAAAATGCAGCGGCAACCCTTTTCAGCCTTTCTGTTGTGGATGAAAACAAGGTTAGAATTGGTGCTTCGGGAGCCATTCCGCCACTTGTTACACTACTGAGTGAAGGTACTCAAAGGGGGAAAAAGGATGCTGCAACAGCACTATTTAACTTGTGCATTTATCAGGGGAACAAGGGGAAAGCAGTAAGGGCTGGTGTTGTTTCAACACTAATGGAGCTGCTAACTGAAACAGGAGGGGGAATGGTGGATGAAGCACTGGCCATTCTAGCAATACTAGCTAGCCATCCTGAAGGGAAAGCAGCCATTGGAGCTGCCGAGGCAGTGCCGGTTTTGGTTGAAGTCATCGGGACTGGATCTCCCCGAAACCGAGAAAATGCAGCTGCAGTTTTGGTGCACCTTTGTTCTGGGGACCCACAACATTTGGTGGATGCTCAGGAACTAGGAGTGATGGCTTCATTATTGGATTTGGCACAAAATGGCACAGACAGAGGCAAGAGAAAGGCTGCACAGTTGCTTGAGAGAATGAATCGGTTTATTGAGCAGCAAGCTCAAGCTGAGGCCGAAGTTGAGACTGAGACTGAGGTCGAGGTTGAGGCTGTGATTGAACCTCGACCACTGGCACAATCTGAGACTCAGCAGCCACAGCCACTGTCTGCAGTGAACGCTGATGACAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003735 GO:0003824 GO:0004842 GO:0005102 GO:0005198 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005840 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0010941 GO:0015934 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019843 GO:0022625 GO:0022626 GO:0031347 GO:0031348 GO:0032446 GO:0032991 GO:0033612 GO:0036211 GO:0042742 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044391 GO:0044422 GO:0044424 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0048586 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0060548 GO:0065007 GO:0070647 GO:0070696 GO:0071704 GO:0080134 GO:0097159 GO:0098542 GO:0140096 GO:1901363 GO:1901564 GO:1990904 GO:2000026 GO:2000028 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

677

Amino Acids

73.54

Weight (kDa)

5.12

Isoelectric Point (pI)

39.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MLKL_N	PF22215	28 - 117	6.5e-09	Mixed lineage kinase domain-like N-terminal domain
PUB10_N	PF25368	30 - 142	5.2e-38	PUB10-like, N-terminal domain
U-box	PF04564	258 - 328	6.8e-22	U-box domain
zf-RING_UBOX	PF13445	264 - 300	4e-06	RING-type zinc-finger
ARM_PUB	PF25598	354 - 634	2.3e-29	Plant U-box protein ARM repeat region
ARM_ARMC5	PF24768	364 - 528	3.5e-10	ARMC5
Arm	PF00514	383 - 422	2.4e-10	Armadillo/beta-catenin-like repeat
ARM_ARMC5	PF24768	420 - 612	1.6e-09	ARMC5
Arm	PF00514	466 - 504	3.9e-07	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010178)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46510
fragaria_vesca	FvH4_3g35800 FvH4_3g35800 FvH4_3g35800
malus_domestica	MD03G1096300.v1.1 MD11G1111000.v1.1
prunus_persica	Prupe.6G079400_v2.0.a1 Prupe.6G079400_v2.0.a1 Prupe.6G079400_v2.0.a1
pyrus_communis	pycom03g07890 pycom11g09180
rosa_chinensis	RchiOBHm_Chr5g0063991
rosa_laevigata	RLG00000035696
rosa_multiflora	Rmu_sc0008881.1_g000007
rosa_roxburghii	Rroxscaffold_1G00016810
rosa_rugosa	Rorug05G0361600 Rorug05G0361700
rosa_samantha	Rh5BG435800 Rh5CG458900 Rh5DG449000
rosa_wichuraiana	Rw5G039470

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	1046
AciI	CCGC	7 cut(s)	167, 1044, 1046, 1129, 1225, 1367, 1436
AclI	AACGTT	1 cut(s)	853
AclWI	GGATC	7 cut(s)	471, 497, 605, 618, 812, 825, 1717
AcoI	YGGCCR	1 cut(s)	1614
AcsI	RAATTY	1 cut(s)	345
AcuI	CTGAAG	3 cut(s)	1119, 1194, 1665
AfaI	GTAC	4 cut(s)	98, 669, 1054, 1465
AfeI	AGCGCT	1 cut(s)	216
AfiI	CCNNNNNNNGG	2 cut(s)	277, 750
AflIII	ACRYGT	1 cut(s)	893
AhdI	GACNNNNNGTC	1 cut(s)	1108
AjnI	CCWGG	4 cut(s)	270, 276, 613, 1312
Alw21I	GWGCWC	3 cut(s)	22, 1332, 1752
Alw26I	GTCTC	5 cut(s)	35, 935, 1919, 1925, 1979
Alw44I	GTGCAC	1 cut(s)	1748
AlwI	GGATC	7 cut(s)	471, 497, 605, 618, 812, 825, 1717
AlwNI	CAGNNNCTG	3 cut(s)	34, 1313, 2005
Aor51HI	AGCGCT	1 cut(s)	216
AoxI	GGCC	6 cut(s)	108, 420, 762, 1004, 1614, 1914
ApaLI	GTGCAC	1 cut(s)	1748
ApoI	RAATTY	1 cut(s)	345
Asp700I	GAANNNNTTC	2 cut(s)	302, 1068
AspLEI	GCGC	2 cut(s)	217, 1252
AspS9I	GGNCC	6 cut(s)	109, 274, 420, 763, 1005, 1765
AsuC2I	CCSGG	1 cut(s)	112
AsuHPI	GGTGA	4 cut(s)	97, 325, 566, 1133
AsuNHI	GCTAGC	1 cut(s)	1634
AvaII	GGWCC	2 cut(s)	274, 1765
AxyI	CCTNAGG	1 cut(s)	177
BaeGI	GKGCMC	1 cut(s)	1752
BalI	TGGCCA	1 cut(s)	1616
BamHI	GGATCC	2 cut(s)	610, 817
BarI	GAAGNNNNNNTAC	2 cut(s)	80, 112
BbsI	GAAGAC	1 cut(s)	1107
Bbv12I	GWGCWC	3 cut(s)	22, 1332, 1752
BbvCI	CCTCAGC	1 cut(s)	1911
BccI	CCATC	3 cut(s)	583, 1647, 1798
BceAI	ACGGC	1 cut(s)	1263
BciT130I	CCWGG	4 cut(s)	272, 278, 615, 1314
BcnI	CCSGG	1 cut(s)	112
BcoDI	GTCTC	5 cut(s)	35, 935, 1919, 1925, 1979
BfaI	CTAG	6 cut(s)	600, 921, 1622, 1631, 1635, 1796
BfmI	CTRYAG	4 cut(s)	73, 702, 1737, 2010
BfoI	RGCGCY	2 cut(s)	218, 1253
BmcAI	AGTACT	1 cut(s)	98
Bme1390I	CCNGG	5 cut(s)	112, 272, 278, 615, 1314
Bme18I	GGWCC	2 cut(s)	274, 1765
BmeRI	GACNNNNNGTC	1 cut(s)	1108
BmgT120I	GGNCC	6 cut(s)	109, 274, 420, 763, 1005, 1765
BmiI	GGNNCC	6 cut(s)	612, 765, 819, 1429, 1766, 1767
BmrFI	CCNGG	5 cut(s)	112, 272, 278, 615, 1314
BmrI	ACTGGG	2 cut(s)	761, 961
BmsI	GCATC	8 cut(s)	68, 481, 682, 749, 1037, 1299, 1474, 1774
BmtI	GCTAGC	1 cut(s)	1638
BmuI	ACTGGG	2 cut(s)	761, 961
BoxI	GACNNNNGTC	1 cut(s)	1937
BpiI	GAAGAC	1 cut(s)	1107
BplI	GAGNNNNNCTC	2 cut(s)	452, 484
BpmI	CTGGAG	1 cut(s)	636
Bpu10I	CCTNAGC	3 cut(s)	228, 1788, 1911
BpuEI	CTTGAG	3 cut(s)	1316, 1890, 1892
BpuMI	CCSGG	1 cut(s)	112
BsaAI	YACGTR	1 cut(s)	953
BsaJI	CCNNGG	2 cut(s)	1044, 1671
BsaXI	ACNNNNNCTCC	2 cut(s)	1696, 1726
Bsc4I	CCNNNNNNNGG	2 cut(s)	277, 750
Bse118I	RCCGGY	1 cut(s)	1681
Bse1I	ACTGG	9 cut(s)	528, 729, 767, 821, 927, 967, 1617, 1711, 1977
Bse21I	CCTNAGG	1 cut(s)	177
Bse3DI	GCAATG	1 cut(s)	578
BseBI	CCWGG	4 cut(s)	272, 278, 615, 1314
BseDI	CCNNGG	2 cut(s)	1044, 1671
BseGI	GGATG	7 cut(s)	697, 740, 1402, 1489, 1609, 1639, 1789
BseLI	CCNNNNNNNGG	2 cut(s)	277, 750
BseMI	GCAATG	1 cut(s)	578
BseNI	ACTGG	9 cut(s)	528, 729, 767, 821, 927, 967, 1617, 1711, 1977
BseRI	GAGGAG	1 cut(s)	158
BseSI	GKGCMC	1 cut(s)	1752
BsgI	GTGCAG	2 cut(s)	47, 1845
Bsh1236I	CGCG	1 cut(s)	1046
BshFI	GGCC	6 cut(s)	110, 422, 764, 1006, 1616, 1916
BsiHKAI	GWGCWC	3 cut(s)	22, 1332, 1752
BsiSI	CCGG	2 cut(s)	112, 1682
BslFI	GGGAC	3 cut(s)	980, 1717, 1778
BslI	CCNNNNNNNGG	2 cut(s)	277, 750
BsmAI	GTCTC	5 cut(s)	35, 935, 1919, 1925, 1979
BsmFI	GGGAC	3 cut(s)	980, 1717, 1778
BsnI	GGCC	6 cut(s)	110, 422, 764, 1006, 1616, 1916
Bsp1286I	GDGCHC	3 cut(s)	22, 1332, 1752
Bsp1407I	TGTACA	1 cut(s)	667
Bsp143I	GATC	6 cut(s)	63, 463, 502, 610, 817, 1709
BspACI	CCGC	7 cut(s)	167, 1044, 1046, 1129, 1225, 1367, 1436
BspANI	GGCC	6 cut(s)	110, 422, 764, 1006, 1616, 1916
BspFNI	CGCG	1 cut(s)	1046
BspLI	GGNNCC	6 cut(s)	612, 765, 819, 1429, 1766, 1767
BspMAI	CTGCAG	2 cut(s)	1741, 2014
BspOI	GCTAGC	1 cut(s)	1638
BspPI	GGATC	7 cut(s)	471, 497, 605, 618, 812, 825, 1717
BspQI	GCTCTTC	1 cut(s)	351
BsrDI	GCAATG	1 cut(s)	578
BsrFI	RCCGGY	1 cut(s)	1681
BsrGI	TGTACA	1 cut(s)	667
BsrI	ACTGG	9 cut(s)	528, 729, 767, 821, 927, 967, 1617, 1711, 1977
BssAI	RCCGGY	1 cut(s)	1681
BssECI	CCNNGG	2 cut(s)	1044, 1671
BssMI	GATC	6 cut(s)	63, 463, 502, 610, 817, 1709
Bst2UI	CCWGG	4 cut(s)	272, 278, 615, 1314
Bst4CI	ACNGT	7 cut(s)	46, 85, 101, 187, 1340, 1866, 2007
Bst6I	CTCTTC	2 cut(s)	351, 424
BstAUI	TGTACA	1 cut(s)	667
BstBAI	YACGTR	1 cut(s)	953
BstC8I	GCNNGC	8 cut(s)	405, 622, 1036, 1040, 1044, 1239, 1636, 1902
BstDSI	CCRYGG	1 cut(s)	1044
BstF5I	GGATG	7 cut(s)	697, 740, 1402, 1489, 1609, 1639, 1789
BstFNI	CGCG	1 cut(s)	1046
BstH2I	RGCGCY	2 cut(s)	218, 1253
BstHHI	GCGC	2 cut(s)	217, 1252
BstKTI	GATC	6 cut(s)	66, 466, 505, 613, 820, 1712
BstMAI	GTCTC	5 cut(s)	35, 935, 1919, 1925, 1979
BstMBI	GATC	6 cut(s)	63, 463, 502, 610, 817, 1709
BstNI	CCWGG	4 cut(s)	272, 278, 615, 1314
BstNSI	RCATGY	3 cut(s)	890, 897, 1241
BstPAI	GACNNNNGTC	1 cut(s)	1937
BstSCI	CCNGG	5 cut(s)	110, 270, 276, 613, 1312
BstSFI	CTRYAG	4 cut(s)	73, 702, 1737, 2010
BstSLI	GKGCMC	1 cut(s)	1752
BstSNI	TACGTA	1 cut(s)	953
BstUI	CGCG	1 cut(s)	1046
BstV2I	GAAGAC	1 cut(s)	1107
BstX2I	RGATCY	5 cut(s)	463, 502, 610, 817, 1709
BstYI	RGATCY	5 cut(s)	463, 502, 610, 817, 1709
Bsu36I	CCTNAGG	1 cut(s)	177
BsuRI	GGCC	6 cut(s)	110, 422, 764, 1006, 1616, 1916
BtgI	CCRYGG	1 cut(s)	1044
BtsCI	GGATG	7 cut(s)	697, 740, 1402, 1489, 1609, 1639, 1789
BtsI	GCAGTG	2 cut(s)	1683, 2019
BtsIMutI	CAGTG	9 cut(s)	183, 736, 744, 974, 1610, 1683, 1970, 2003, 2019
Cac8I	GCNNGC	8 cut(s)	405, 622, 1036, 1040, 1044, 1239, 1636, 1902
CaiI	CAGNNNCTG	3 cut(s)	34, 1313, 2005
CfoI	GCGC	2 cut(s)	217, 1252
Cfr10I	RCCGGY	1 cut(s)	1681
Cfr13I	GGNCC	6 cut(s)	109, 274, 420, 763, 1005, 1765
Cfr42I	CCGCGG	1 cut(s)	1047
CsiI	ACCWGGT	1 cut(s)	270
Csp6I	GTAC	4 cut(s)	97, 668, 1053, 1464
CspCI	CAANNNNNGTGG	4 cut(s)	1321, 1356, 1758, 1793
CviAII	CATG	8 cut(s)	308, 584, 688, 887, 894, 1238, 1325, 1345
CviQI	GTAC	4 cut(s)	97, 668, 1053, 1464
DpnI	GATC	6 cut(s)	65, 465, 504, 612, 819, 1711
DpnII	GATC	6 cut(s)	63, 463, 502, 610, 817, 1709
DriI	GACNNNNNGTC	1 cut(s)	1108
EaeI	YGGCCR	1 cut(s)	1614
Eam1104I	CTCTTC	2 cut(s)	351, 424
Eam1105I	GACNNNNNGTC	1 cut(s)	1108
EarI	CTCTTC	2 cut(s)	351, 424
EciI	GGCGGA	2 cut(s)	1118, 1425
Eco105I	TACGTA	1 cut(s)	953
Eco47I	GGWCC	2 cut(s)	274, 1765
Eco47III	AGCGCT	1 cut(s)	216
Eco57I	CTGAAG	3 cut(s)	1119, 1194, 1665
Eco81I	CCTNAGG	1 cut(s)	177
EcoO109I	RGGNCCY	2 cut(s)	763, 1765
EcoRII	CCWGG	4 cut(s)	270, 276, 613, 1312
FaeI	CATG	8 cut(s)	311, 587, 691, 890, 897, 1241, 1328, 1348
FalI	AAGNNNNNCTT	2 cut(s)	558, 590
FaqI	GGGAC	3 cut(s)	980, 1717, 1778
FatI	CATG	8 cut(s)	307, 583, 687, 886, 893, 1237, 1324, 1344
FauI	CCCGC	2 cut(s)	1051, 1232
FauNDI	CATATG	1 cut(s)	847
FokI	GGATG	7 cut(s)	704, 727, 1409, 1496, 1616, 1626, 1796
FspBI	CTAG	6 cut(s)	600, 921, 1622, 1631, 1635, 1796
GlaI	GCGC	2 cut(s)	216, 1251
GsuI	CTGGAG	1 cut(s)	636
HaeII	RGCGCY	2 cut(s)	218, 1253
HaeIII	GGCC	6 cut(s)	110, 422, 764, 1006, 1616, 1916
HapII	CCGG	2 cut(s)	112, 1682
HhaI	GCGC	2 cut(s)	217, 1252
Hin1II	CATG	8 cut(s)	311, 587, 691, 890, 897, 1241, 1328, 1348
Hin6I	GCGC	2 cut(s)	215, 1250
HinP1I	GCGC	2 cut(s)	215, 1250
HincII	GTYRAC	1 cut(s)	49
HindII	GTYRAC	1 cut(s)	49
HindIII	AAGCTT	1 cut(s)	192
HinfI	GANTC	8 cut(s)	130, 221, 243, 569, 754, 1220, 1882, 1987
HpaII	CCGG	2 cut(s)	112, 1682
HphI	GGTGA	4 cut(s)	97, 325, 566, 1133
Hpy166II	GTNNAC	5 cut(s)	49, 557, 734, 1750, 2017
Hpy188I	TCNGA	5 cut(s)	70, 242, 373, 900, 1984
Hpy188III	TCNNGA	4 cut(s)	1425, 1643, 1702, 1790
Hpy8I	GTNNAC	5 cut(s)	49, 557, 734, 1750, 2017
HpyAV	CCTTC	6 cut(s)	376, 411, 808, 1142, 1454, 1640
HpyCH4III	ACNGT	7 cut(s)	46, 85, 101, 187, 1340, 1866, 2007
HpyCH4IV	ACGT	3 cut(s)	853, 952, 1051
HpySE526I	ACGT	3 cut(s)	853, 952, 1051
Hsp92II	CATG	8 cut(s)	311, 587, 691, 890, 897, 1241, 1328, 1348
HspAI	GCGC	2 cut(s)	215, 1250
KflI	GGGWCCC	1 cut(s)	1765
KspI	CCGCGG	1 cut(s)	1047
Kzo9I	GATC	6 cut(s)	63, 463, 502, 610, 817, 1709
LguI	GCTCTTC	1 cut(s)	351
LmnI	GCTCC	6 cut(s)	617, 700, 1304, 1427, 1570, 1664
LweI	GCATC	8 cut(s)	68, 481, 682, 749, 1037, 1299, 1474, 1774
MabI	ACCWGGT	1 cut(s)	270
MaeI	CTAG	6 cut(s)	600, 921, 1622, 1631, 1635, 1796
MaeII	ACGT	3 cut(s)	853, 952, 1051
MaeIII	GTNAC	6 cut(s)	313, 534, 570, 889, 1243, 1444
MalI	GATC	6 cut(s)	65, 465, 504, 612, 819, 1711
MboI	GATC	6 cut(s)	63, 463, 502, 610, 817, 1709
MboII	GAAGA	7 cut(s)	20, 100, 368, 441, 1061, 1064, 1112
MfeI	CAATTG	2 cut(s)	539, 1167
MflI	RGATCY	5 cut(s)	463, 502, 610, 817, 1709
MhlI	GDGCHC	3 cut(s)	22, 1332, 1752
MlsI	TGGCCA	1 cut(s)	1616
MluCI	AATT	6 cut(s)	103, 345, 539, 806, 1167, 1413
MluNI	TGGCCA	1 cut(s)	1616
MlyI	GAGTC	3 cut(s)	124, 578, 1981
MmeI	TCCRAC	1 cut(s)	93
Mox20I	TGGCCA	1 cut(s)	1616
MroXI	GAANNNNTTC	2 cut(s)	302, 1068
MscI	TGGCCA	1 cut(s)	1616
MseI	TTAA	3 cut(s)	338, 657, 1503
Msp20I	TGGCCA	1 cut(s)	1616
MspA1I	CMGCKG	4 cut(s)	1046, 1367, 1736, 2031
MspI	CCGG	2 cut(s)	112, 1682
MspR9I	CCNGG	5 cut(s)	112, 272, 278, 615, 1314
MunI	CAATTG	2 cut(s)	539, 1167
MvaI	CCWGG	4 cut(s)	272, 278, 615, 1314
MvnI	CGCG	1 cut(s)	1046
NciI	CCSGG	1 cut(s)	112
NdeI	CATATG	1 cut(s)	847
NdeII	GATC	6 cut(s)	63, 463, 502, 610, 817, 1709
NheI	GCTAGC	1 cut(s)	1634
NlaIII	CATG	8 cut(s)	311, 587, 691, 890, 897, 1241, 1328, 1348
NlaIV	GGNNCC	6 cut(s)	612, 765, 819, 1429, 1766, 1767
NmeAIII	GCCGAG	1 cut(s)	1696
NmuCI	GTSAC	3 cut(s)	313, 570, 1243
NspI	RCATGY	3 cut(s)	890, 897, 1241
PaeI	GCATGC	1 cut(s)	1241
PciI	ACATGT	1 cut(s)	893
PciSI	GCTCTTC	1 cut(s)	351
PdmI	GAANNNNTTC	2 cut(s)	302, 1068
PfeI	GAWTC	5 cut(s)	221, 243, 754, 1220, 1882
PfoI	TCCNGGA	2 cut(s)	276, 613
PleI	GAGTC	3 cut(s)	124, 577, 1981
PpsI	GAGTC	3 cut(s)	124, 577, 1981
Ppu21I	YACGTR	1 cut(s)	953
PpuMI	RGGWCCY	1 cut(s)	1765
PscI	ACATGT	1 cut(s)	893
PshAI	GACNNNNGTC	1 cut(s)	1937
Psp1406I	AACGTT	1 cut(s)	853
Psp5II	RGGWCCY	1 cut(s)	1765
Psp6I	CCWGG	4 cut(s)	270, 276, 613, 1312
PspGI	CCWGG	4 cut(s)	270, 276, 613, 1312
PspN4I	GGNNCC	6 cut(s)	612, 765, 819, 1429, 1766, 1767
PspPI	GGNCC	6 cut(s)	109, 274, 420, 763, 1005, 1765
PspPPI	RGGWCCY	1 cut(s)	1765
PstI	CTGCAG	2 cut(s)	1741, 2014
PstNI	CAGNNNCTG	3 cut(s)	34, 1313, 2005
PsuI	RGATCY	5 cut(s)	463, 502, 610, 817, 1709
PvuII	CAGCTG	2 cut(s)	1736, 2031
RsaI	GTAC	4 cut(s)	98, 669, 1054, 1465
RsaNI	GTAC	4 cut(s)	97, 668, 1053, 1464
SacII	CCGCGG	1 cut(s)	1047
SapI	GCTCTTC	1 cut(s)	351
SaqAI	TTAA	3 cut(s)	338, 657, 1503
Sau3AI	GATC	6 cut(s)	63, 463, 502, 610, 817, 1709
Sau96I	GGNCC	6 cut(s)	109, 274, 420, 763, 1005, 1765
ScaI	AGTACT	1 cut(s)	98
SchI	GAGTC	3 cut(s)	124, 578, 1981
ScrFI	CCNGG	5 cut(s)	112, 272, 278, 615, 1314
SduI	GDGCHC	3 cut(s)	22, 1332, 1752
SexAI	ACCWGGT	1 cut(s)	270
SfaNI	GCATC	8 cut(s)	68, 481, 682, 749, 1037, 1299, 1474, 1774
SfcI	CTRYAG	4 cut(s)	73, 702, 1737, 2010
Sfr303I	CCGCGG	1 cut(s)	1047
SgrBI	CCGCGG	1 cut(s)	1047
SinI	GGWCC	2 cut(s)	274, 1765
SmlI	CTYRAG	3 cut(s)	1331, 1871, 1905
SmoI	CTYRAG	3 cut(s)	1331, 1871, 1905
SnaBI	TACGTA	1 cut(s)	953
SphI	GCATGC	1 cut(s)	1241
Sse9I	AATT	6 cut(s)	103, 345, 539, 806, 1167, 1413
SsiI	CCGC	7 cut(s)	167, 1044, 1046, 1129, 1225, 1367, 1436
SspMI	CTAG	6 cut(s)	600, 921, 1622, 1631, 1635, 1796
StyD4I	CCNGG	5 cut(s)	110, 270, 276, 613, 1312
TaaI	ACNGT	7 cut(s)	46, 85, 101, 187, 1340, 1866, 2007
TaiI	ACGT	3 cut(s)	856, 955, 1054
TaqI	TCGA	7 cut(s)	62, 141, 322, 787, 1012, 1941, 1966
TasI	AATT	6 cut(s)	103, 345, 539, 806, 1167, 1413
TatI	WGTACW	2 cut(s)	96, 667
TauI	GCSGC	1 cut(s)	1370
TfiI	GAWTC	5 cut(s)	221, 243, 754, 1220, 1882
Tru1I	TTAA	3 cut(s)	338, 657, 1503
Tru9I	TTAA	3 cut(s)	338, 657, 1503
TscAI	CASTG	9 cut(s)	190, 736, 751, 974, 1617, 1683, 1977, 2010, 2019
TseFI	GTSAC	3 cut(s)	313, 570, 1243
Tsp45I	GTSAC	3 cut(s)	313, 570, 1243
TspDTI	ATGAA	9 cut(s)	311, 390, 827, 864, 1313, 1413, 1620, 1800, 1895
TspRI	CASTG	9 cut(s)	190, 736, 751, 974, 1617, 1683, 1977, 2010, 2019
VneI	GTGCAC	1 cut(s)	1748
VpaK11BI	GGWCC	2 cut(s)	274, 1765
XapI	RAATTY	1 cut(s)	345
XceI	RCATGY	3 cut(s)	890, 897, 1241
XmnI	GAANNNNTTC	2 cut(s)	302, 1068
XspI	CTAG	6 cut(s)	600, 921, 1622, 1631, 1635, 1796
ZrmI	AGTACT	1 cut(s)	98

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.