Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007637

Physical Location & Seq

Genomic Coordinates Reverse (-)

17338682 .. 17344926

Gene Structure 6245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1152700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGTCGGGCTCGCGGGCTCCAAACCCGCCGGCGCAACCGCCACAGCCGGTCATGCAGCAACCCTTGAAGCGGCAACTCCCCTTCTCCTCCATGAGGCCGCCGTTTTCAGCTGCTGCAGACTATCACGGCTTCCTTTCCGACCCCCGCCAAACCACTGATCAAGAAACCTACGGCATAACCGTTAAGACCCCTCTAAAAAGGAAGAGTGATGCAGTAGATTGCGAAGCCGAATCTAGTGATAGGACAACTGCTCCTGGATACACTGACGTAGGTAACAGCCCCCTCCAGACTCCTATGTCAACCAAGGTTGGAAAGGCGAACAAAACATCAAGGCTTACAAAGTGCAGCAAATCTGGACCGCAGACTCCGGCTTCCAATGTGGGTTCTCCTTCTGGCGCTAATGTTACTCCGGCTGGTCCATGTCGTTTTGACAGCTCCCTAGGTCTCTTAACAAAGAAGTTTATCAATCTGATCAAACATGCGGAAGATGGTATTCTAGATCTCAATAAAGCTGCTGATACTTTAGAGGTCCAAAAGAGGCGGATATATGATATAACAAATGTTCTCGAAGGAATTGGTCTCATAGAGAAGAAGCTCAAAAATAGAATTCAGTGGAAGGGACTTGATGTCTCGAGGACAGGAGACGTGGACGAAAATTATCCTAGTTTACAGGCACAAGTTGAAAACCTATCTATTGAGGAGCACAGATTAGATCAGCAAATAAGAGAAATGCAGGAAAGGTTGAGGGACCTAAGCGACAATGAAAGCAATAAGAAATGGCTTTTTGTCACCGAAGAAGACATTAAGGGCTTACCTTGCTTGCAGAATGAAACCTTAATAGCAATAAAAGCTCCACATGGCACCACTCTTGAAGTCCCAGATCCTGACGAGGCTGTTGACTATCCCCAAAGGAGATACAGGATGGTTTTGAGAAGCACAATGGGTCCTATAGATGTTTACCTTGTCAGTCAATTTGAGGAGAAGTTTGAGGAGATTAATGGTGTCGAAGTACCTACAAACATCGCTGCAACTTCAGGTGCTAATGAAAACCCAGCTACAACAATGGTTTCAGAGGATAGAGGGAAGGATGTTGAACTGCAGGTACCAGATGATCATACAATGTCTGCTGATCCTACGGCTGCCCAGGACTTAACTGGTGGGATTATGAAGATTGTTCCCTCAGATGTTGATAGTGATGCAGATTACTGGCTTTTATCAGATGCTGATATTAGTATCACAGACTTGTGGAGAACAGAACCTGGGGTTTTGTGGAATGATTTGGGTGCACTTGATCATGATTATCTGTTGGGTAATGTCAGCACACCACCTCGGCCCCAAACTCCGCCATCCAGCTTAATTGAAGTACCTTCTACTACCAGCAACCCTACGAAGACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0008150 GO:0008284 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010033 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0032989 GO:0042127 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045787 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051302 GO:0051445 GO:0051446 GO:0051726 GO:0060255 GO:0065007 GO:0071840 GO:0080090 GO:0090558 GO:0090626 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2000241 GO:2000243 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko01522 ko03018 ko04110 ko04218 ko04934 ko05161 ko05166 ko05167 ko05200 ko05206 ko05212 ko05214 ko05215 ko05218 ko05219 ko05220 ko05222 ko05223 ko05224 ko05225 ko05226 map01522 map03018 map04110 map04218 map04934 map05161 map05166 map05167 map05200 map05206 map05212 map05214 map05215 map05218 map05219 map05220 map05222 map05223 map05224 map05225 map05226

Pfam Domains

Protein Families

E2F_CC-MB E2F_TDP

Protein Analysis

466

Amino Acids

51.11

Weight (kDa)

4.86

Isoelectric Point (pI)

48.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WHD_E2F_TDP	PF02319	144 - 207	2.5e-27	E2F/DP family winged-helix DNA-binding domain
E2F_CC-MB	PF16421	223 - 323	8.7e-35	E2F transcription factor CC-MB domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007637)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22220 AT5G22220
fragaria_vesca	FvH4_3g25990 FvH4_3g25990 FvH4_3g25990 FvH4_3g25990 FvH4_3g25990
malus_domestica	MD03G1152700.v1.1 MD11G1172700.v1.1
prunus_persica	Prupe.6G139900_v2.0.a1 Prupe.6G139900_v2.0.a1
pyrus_communis	pycom03g10660 pycom11g14570
rosa_chinensis	RchiOBHm_Chr5g0047281
rosa_laevigata	RLG00000034486
rosa_multiflora	Rmu_sc0003154.1_g000003 Rmu_sc0003441.1_g000098
rosa_roxburghii	Rroxscaffold_1G00034080
rosa_rugosa	Rorug05G0235400
rosa_samantha	Rh5AG314400 Rh5BG323100 Rh5CG350300 Rh5DG333900
rosa_wichuraiana	Rw5G029390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	295
Acc36I	ACCTGC	1 cut(s)	1090
Acc65I	GGTACC	1 cut(s)	1102
AccB1I	GGYRCC	2 cut(s)	860, 1102
AccII	CGCG	1 cut(s)	13
AclWI	GGATC	2 cut(s)	875, 1124
AcsI	RAATTY	1 cut(s)	606
AcuI	CTGAAG	1 cut(s)	1017
AfaI	GTAC	3 cut(s)	1011, 1104, 1365
AfiI	CCNNNNNNNGG	2 cut(s)	69, 93
AgsI	TTSAA	5 cut(s)	67, 683, 872, 1094, 1361
AhdI	GACNNNNNGTC	1 cut(s)	626
AjiI	CACGTC	1 cut(s)	646
AjnI	CCWGG	3 cut(s)	253, 1143, 1258
AluBI	AGCT	7 cut(s)	110, 435, 512, 595, 851, 1055, 1353
AluI	AGCT	7 cut(s)	110, 435, 512, 595, 851, 1055, 1353
Alw21I	GWGCWC	2 cut(s)	705, 1288
Alw26I	GTCTC	4 cut(s)	449, 584, 634, 636
Alw44I	GTGCAC	1 cut(s)	1284
AlwI	GGATC	2 cut(s)	875, 1124
AlwNI	CAGNNNCTG	4 cut(s)	113, 884, 1223, 1259
Ama87I	CYCGRG	1 cut(s)	631
AoxI	GGCC	2 cut(s)	95, 1331
ApaLI	GTGCAC	1 cut(s)	1284
ApeKI	GCWGC	7 cut(s)	55, 110, 113, 345, 512, 1025, 1139
ApoI	RAATTY	1 cut(s)	606
AseI	ATTAAT	1 cut(s)	996
Asp718I	GGTACC	1 cut(s)	1102
AspA2I	CCTAGG	1 cut(s)	439
AspLEI	GCGC	2 cut(s)	34, 398
AspS9I	GGNCC	6 cut(s)	356, 416, 529, 748, 944, 1332
AsuHPI	GGTGA	1 cut(s)	781
AvaI	CYCGRG	1 cut(s)	631
AvaII	GGWCC	5 cut(s)	356, 416, 529, 748, 944
AvrII	CCTAGG	1 cut(s)	439
BaeGI	GKGCMC	1 cut(s)	1288
BanI	GGYRCC	2 cut(s)	860, 1102
BanII	GRGCYC	2 cut(s)	11, 19
BbsI	GAAGAC	1 cut(s)	804
Bbv12I	GWGCWC	2 cut(s)	705, 1288
BbvI	GCAGC	7 cut(s)	67, 97, 100, 357, 499, 1012, 1126
BccI	CCATC	3 cut(s)	482, 916, 1354
BceAI	ACGGC	4 cut(s)	85, 142, 187, 1152
BciT130I	CCWGG	3 cut(s)	255, 1145, 1260
BciVI	GTATCC	1 cut(s)	251
BclI	TGATCA	4 cut(s)	157, 471, 1111, 1291
BcoDI	GTCTC	4 cut(s)	449, 584, 634, 636
BfaI	CTAG	4 cut(s)	234, 440, 497, 663
BfmI	CTRYAG	3 cut(s)	114, 948, 1097
BfoI	RGCGCY	1 cut(s)	399
BfuAI	ACCTGC	1 cut(s)	1090
BfuI	GTATCC	1 cut(s)	251
BglII	AGATCT	1 cut(s)	499
BisI	GCNGC	9 cut(s)	56, 71, 98, 111, 114, 346, 513, 1026, 1140
BlnI	CCTAGG	1 cut(s)	439
BlsI	GCNGC	9 cut(s)	57, 72, 99, 112, 115, 347, 514, 1027, 1141
Bme1390I	CCNGG	3 cut(s)	255, 1145, 1260
Bme18I	GGWCC	5 cut(s)	356, 416, 529, 748, 944
BmeRI	GACNNNNNGTC	1 cut(s)	626
BmeT110I	CYCGRG	1 cut(s)	631
BmgBI	CACGTC	1 cut(s)	646
BmgT120I	GGNCC	6 cut(s)	356, 416, 529, 748, 944, 1332
BmiI	GGNNCC	6 cut(s)	18, 749, 862, 945, 1104, 1334
BmrFI	CCNGG	3 cut(s)	255, 1145, 1260
BmsI	GCATC	3 cut(s)	199, 1186, 1210
BpiI	GAAGAC	1 cut(s)	804
BpmI	CTGGAG	1 cut(s)	269
Bpu10I	CCTNAGC	1 cut(s)	752
BsaI	GGTCTC	2 cut(s)	449, 584
BsaJI	CCNNGG	5 cut(s)	303, 439, 1143, 1259, 1328
BsaXI	ACNNNNNCTCC	2 cut(s)	235, 265
Bsc4I	CCNNNNNNNGG	2 cut(s)	69, 93
Bse118I	RCCGGY	2 cut(s)	28, 46
Bse1I	ACTGG	2 cut(s)	1159, 1211
BseBI	CCWGG	3 cut(s)	255, 1145, 1260
BseDI	CCNNGG	5 cut(s)	303, 439, 1143, 1259, 1328
BseGI	GGATG	3 cut(s)	927, 1093, 1346
BseLI	CCNNNNNNNGG	2 cut(s)	69, 93
BseMII	CTCAG	1 cut(s)	1194
BseNI	ACTGG	2 cut(s)	1159, 1211
BseRI	GAGGAG	4 cut(s)	76, 713, 992, 1004
BseSI	GKGCMC	1 cut(s)	1288
BseXI	GCAGC	7 cut(s)	67, 97, 100, 357, 499, 1012, 1126
BseYI	CCCAGC	1 cut(s)	1051
BsgI	GTGCAG	1 cut(s)	364
Bsh1236I	CGCG	1 cut(s)	13
BshFI	GGCC	2 cut(s)	97, 1333
BshNI	GGYRCC	2 cut(s)	860, 1102
BsiHKAI	GWGCWC	2 cut(s)	705, 1288
BsiHKCI	CYCGRG	1 cut(s)	631
BsiSI	CCGG	4 cut(s)	29, 47, 368, 410
BslFI	GGGAC	3 cut(s)	633, 761, 860
BslI	CCNNNNNNNGG	2 cut(s)	69, 93
BsmAI	GTCTC	4 cut(s)	449, 584, 634, 636
BsmBI	CGTCTC	1 cut(s)	636
BsmFI	GGGAC	3 cut(s)	633, 761, 860
BsnI	GGCC	2 cut(s)	97, 1333
Bso31I	GGTCTC	2 cut(s)	449, 584
BsoBI	CYCGRG	1 cut(s)	631
Bsp1286I	GDGCHC	4 cut(s)	11, 19, 705, 1288
Bsp143I	GATC	8 cut(s)	157, 471, 499, 712, 880, 1111, 1129, 1291
BspANI	GGCC	2 cut(s)	97, 1333
BspCNI	CTCAG	1 cut(s)	1193
BspFNI	CGCG	1 cut(s)	13
BspHI	TCATGA	1 cut(s)	1294
BspLI	GGNNCC	6 cut(s)	18, 749, 862, 945, 1104, 1334
BspMAI	CTGCAG	2 cut(s)	118, 1101
BspMI	ACCTGC	1 cut(s)	1090
BspPI	GGATC	2 cut(s)	875, 1124
BspT107I	GGYRCC	2 cut(s)	860, 1102
BspTNI	GGTCTC	2 cut(s)	449, 584
BsrFI	RCCGGY	2 cut(s)	28, 46
BsrI	ACTGG	2 cut(s)	1159, 1211
BssAI	RCCGGY	2 cut(s)	28, 46
BssECI	CCNNGG	5 cut(s)	303, 439, 1143, 1259, 1328
BssMI	GATC	8 cut(s)	157, 471, 499, 712, 880, 1111, 1129, 1291
BssT1I	CCWWGG	2 cut(s)	303, 439
Bst2UI	CCWGG	3 cut(s)	255, 1145, 1260
Bst4CI	ACNGT	1 cut(s)	181
Bst6I	CTCTTC	1 cut(s)	197
BstC8I	GCNNGC	4 cut(s)	11, 15, 30, 821
BstDEI	CTNAG	2 cut(s)	752, 1180
BstENI	CCTNNNNNAGG	1 cut(s)	91
BstF5I	GGATG	3 cut(s)	927, 1093, 1346
BstFNI	CGCG	1 cut(s)	13
BstH2I	RGCGCY	1 cut(s)	399
BstHHI	GCGC	2 cut(s)	34, 398
BstKTI	GATC	8 cut(s)	160, 474, 502, 715, 883, 1114, 1132, 1294
BstMAI	GTCTC	4 cut(s)	449, 584, 634, 636
BstMBI	GATC	8 cut(s)	157, 471, 499, 712, 880, 1111, 1129, 1291
BstMWI	GCNNNNNNNGC	3 cut(s)	52, 816, 848
BstNI	CCWGG	3 cut(s)	255, 1145, 1260
BstNSI	RCATGY	1 cut(s)	482
BstSCI	CCNGG	3 cut(s)	253, 1143, 1258
BstSFI	CTRYAG	3 cut(s)	114, 948, 1097
BstSLI	GKGCMC	1 cut(s)	1288
BstUI	CGCG	1 cut(s)	13
BstV1I	GCAGC	7 cut(s)	67, 97, 100, 357, 499, 1012, 1126
BstV2I	GAAGAC	1 cut(s)	804
BstX2I	RGATCY	2 cut(s)	499, 880
BstYI	RGATCY	2 cut(s)	499, 880
BsuI	GTATCC	1 cut(s)	251
BsuRI	GGCC	2 cut(s)	97, 1333
BtgZI	GCGATG	1 cut(s)	1006
BtrI	CACGTC	1 cut(s)	646
BtsCI	GGATG	3 cut(s)	927, 1093, 1346
BtsIMutI	CAGTG	3 cut(s)	153, 261, 617
BveI	ACCTGC	1 cut(s)	1090
Cac8I	GCNNGC	4 cut(s)	11, 15, 30, 821
CaiI	CAGNNNCTG	4 cut(s)	113, 884, 1223, 1259
CciI	TCATGA	1 cut(s)	1294
CfoI	GCGC	2 cut(s)	34, 398
Cfr10I	RCCGGY	2 cut(s)	28, 46
Cfr13I	GGNCC	6 cut(s)	356, 416, 529, 748, 944, 1332
Csp6I	GTAC	3 cut(s)	1010, 1103, 1364
CviAII	CATG	6 cut(s)	52, 91, 420, 479, 857, 1295
CviQI	GTAC	3 cut(s)	1010, 1103, 1364
DdeI	CTNAG	2 cut(s)	752, 1180
DpnI	GATC	8 cut(s)	159, 473, 501, 714, 882, 1113, 1131, 1293
DpnII	GATC	8 cut(s)	157, 471, 499, 712, 880, 1111, 1129, 1291
DrdI	GACNNNNNNGTC	1 cut(s)	295
DriI	GACNNNNNGTC	1 cut(s)	626
DseDI	GACNNNNNNGTC	1 cut(s)	295
Eam1104I	CTCTTC	1 cut(s)	197
Eam1105I	GACNNNNNGTC	1 cut(s)	626
EarI	CTCTTC	1 cut(s)	197
EciI	GGCGGA	2 cut(s)	556, 1332
Eco130I	CCWWGG	2 cut(s)	303, 439
Eco24I	GRGCYC	2 cut(s)	11, 19
Eco31I	GGTCTC	2 cut(s)	449, 584
Eco47I	GGWCC	5 cut(s)	356, 416, 529, 748, 944
Eco57I	CTGAAG	1 cut(s)	1017
Eco88I	CYCGRG	1 cut(s)	631
EcoNI	CCTNNNNNAGG	1 cut(s)	91
EcoO109I	RGGNCCY	2 cut(s)	748, 944
EcoRI	GAATTC	1 cut(s)	606
EcoRII	CCWGG	3 cut(s)	253, 1143, 1258
EcoT14I	CCWWGG	2 cut(s)	303, 439
EcoT38I	GRGCYC	2 cut(s)	11, 19
ErhI	CCWWGG	2 cut(s)	303, 439
Esp3I	CGTCTC	1 cut(s)	636
FaeI	CATG	6 cut(s)	55, 94, 423, 482, 860, 1298
FaqI	GGGAC	3 cut(s)	633, 761, 860
FatI	CATG	6 cut(s)	51, 90, 419, 478, 856, 1294
FauI	CCCGC	3 cut(s)	6, 33, 152
FbaI	TGATCA	4 cut(s)	157, 471, 1111, 1291
Fnu4HI	GCNGC	9 cut(s)	56, 71, 98, 111, 114, 346, 513, 1026, 1140
FokI	GGATG	3 cut(s)	934, 1100, 1333
FriOI	GRGCYC	2 cut(s)	11, 19
Fsp4HI	GCNGC	9 cut(s)	56, 71, 98, 111, 114, 346, 513, 1026, 1140
FspBI	CTAG	4 cut(s)	234, 440, 497, 663
GlaI	GCGC	2 cut(s)	33, 397
GluI	GCNGC	9 cut(s)	56, 71, 98, 111, 114, 346, 513, 1026, 1140
GsaI	CCCAGC	1 cut(s)	1055
GsuI	CTGGAG	1 cut(s)	269
HaeII	RGCGCY	1 cut(s)	399
HaeIII	GGCC	2 cut(s)	97, 1333
HapII	CCGG	4 cut(s)	29, 47, 368, 410
HhaI	GCGC	2 cut(s)	34, 398
Hin1II	CATG	6 cut(s)	55, 94, 423, 482, 860, 1298
Hin6I	GCGC	2 cut(s)	32, 396
HinP1I	GCGC	2 cut(s)	32, 396
HincII	GTYRAC	2 cut(s)	300, 898
HindII	GTYRAC	2 cut(s)	300, 898
HinfI	GANTC	3 cut(s)	230, 289, 364
HpaII	CCGG	4 cut(s)	29, 47, 368, 410
HphI	GGTGA	1 cut(s)	781
Hpy166II	GTNNAC	6 cut(s)	300, 649, 668, 898, 958, 1286
Hpy188I	TCNGA	5 cut(s)	139, 471, 1072, 1183, 1219
Hpy188III	TCNNGA	9 cut(s)	161, 286, 354, 497, 566, 631, 869, 884, 1295
Hpy8I	GTNNAC	6 cut(s)	300, 649, 668, 898, 958, 1286
HpyAV	CCTTC	6 cut(s)	91, 399, 563, 610, 1078, 1377
HpyCH4III	ACNGT	1 cut(s)	181
HpyCH4IV	ACGT	2 cut(s)	267, 645
HpyF10VI	GCNNNNNNNGC	3 cut(s)	52, 816, 848
HpyF3I	CTNAG	2 cut(s)	752, 1180
HpySE526I	ACGT	2 cut(s)	267, 645
Hsp92II	CATG	6 cut(s)	55, 94, 423, 482, 860, 1298
HspAI	GCGC	2 cut(s)	32, 396
KpnI	GGTACC	1 cut(s)	1106
KroI	GCCGGC	1 cut(s)	28
KroNI	GCCGGC	1 cut(s)	30
Ksp22I	TGATCA	4 cut(s)	157, 471, 1111, 1291
Kzo9I	GATC	8 cut(s)	157, 471, 499, 712, 880, 1111, 1129, 1291
LmnI	GCTCC	5 cut(s)	22, 256, 440, 700, 856
Lsp1109I	GCAGC	7 cut(s)	67, 97, 100, 357, 499, 1012, 1126
LweI	GCATC	3 cut(s)	199, 1186, 1210
MaeI	CTAG	4 cut(s)	234, 440, 497, 663
MaeII	ACGT	2 cut(s)	267, 645
MaeIII	GTNAC	3 cut(s)	272, 403, 787
MalI	GATC	8 cut(s)	159, 473, 501, 714, 882, 1113, 1131, 1293
MboI	GATC	8 cut(s)	157, 471, 499, 712, 880, 1111, 1129, 1291
MboII	GAAGA	6 cut(s)	214, 497, 601, 806, 809, 1180
MflI	RGATCY	2 cut(s)	499, 880
MhlI	GDGCHC	4 cut(s)	11, 19, 705, 1288
MluCI	AATT	5 cut(s)	573, 606, 655, 971, 1356
MlyI	GAGTC	2 cut(s)	283, 358
MmeI	TCCRAC	2 cut(s)	162, 289
MreI	CGCCGGCG	1 cut(s)	28
MroNI	GCCGGC	1 cut(s)	28
MseI	TTAA	7 cut(s)	183, 449, 804, 836, 996, 1151, 1355
MspA1I	CMGCKG	1 cut(s)	110
MspI	CCGG	4 cut(s)	29, 47, 368, 410
MspR9I	CCNGG	3 cut(s)	255, 1145, 1260
MvaI	CCWGG	3 cut(s)	255, 1145, 1260
MvnI	CGCG	1 cut(s)	13
MwoI	GCNNNNNNNGC	3 cut(s)	52, 816, 848
NaeI	GCCGGC	1 cut(s)	30
NdeII	GATC	8 cut(s)	157, 471, 499, 712, 880, 1111, 1129, 1291
NgoMIV	GCCGGC	1 cut(s)	28
NlaIII	CATG	6 cut(s)	55, 94, 423, 482, 860, 1298
NlaIV	GGNNCC	6 cut(s)	18, 749, 862, 945, 1104, 1334
NmeAIII	GCCGAG	1 cut(s)	1309
NmuCI	GTSAC	1 cut(s)	787
NspI	RCATGY	1 cut(s)	482
PaeR7I	CTCGAG	1 cut(s)	631
PagI	TCATGA	1 cut(s)	1294
PcsI	WCGNNNNNNNCGW	1 cut(s)	177
PdiI	GCCGGC	1 cut(s)	30
PfeI	GAWTC	1 cut(s)	230
PfoI	TCCNGGA	1 cut(s)	253
PkrI	GCNGC	9 cut(s)	57, 72, 99, 112, 115, 347, 514, 1027, 1141
PleI	GAGTC	2 cut(s)	283, 358
PpsI	GAGTC	2 cut(s)	283, 358
PpuMI	RGGWCCY	2 cut(s)	748, 944
PshBI	ATTAAT	1 cut(s)	996
Psp5II	RGGWCCY	2 cut(s)	748, 944
Psp6I	CCWGG	3 cut(s)	253, 1143, 1258
PspFI	CCCAGC	1 cut(s)	1051
PspGI	CCWGG	3 cut(s)	253, 1143, 1258
PspN4I	GGNNCC	6 cut(s)	18, 749, 862, 945, 1104, 1334
PspPI	GGNCC	6 cut(s)	356, 416, 529, 748, 944, 1332
PspPPI	RGGWCCY	2 cut(s)	748, 944
PsrI	GAACNNNNNNTAC	2 cut(s)	1086, 1118
PstI	CTGCAG	2 cut(s)	118, 1101
PstNI	CAGNNNCTG	4 cut(s)	113, 884, 1223, 1259
PsuI	RGATCY	2 cut(s)	499, 880
PvuII	CAGCTG	1 cut(s)	110
RsaI	GTAC	3 cut(s)	1011, 1104, 1365
RsaNI	GTAC	3 cut(s)	1010, 1103, 1364
SaqAI	TTAA	7 cut(s)	183, 449, 804, 836, 996, 1151, 1355
SatI	GCNGC	9 cut(s)	56, 71, 98, 111, 114, 346, 513, 1026, 1140
Sau3AI	GATC	8 cut(s)	157, 471, 499, 712, 880, 1111, 1129, 1291
Sau96I	GGNCC	6 cut(s)	356, 416, 529, 748, 944, 1332
SchI	GAGTC	2 cut(s)	283, 358
ScrFI	CCNGG	3 cut(s)	255, 1145, 1260
SduI	GDGCHC	4 cut(s)	11, 19, 705, 1288
SfaNI	GCATC	3 cut(s)	199, 1186, 1210
SfcI	CTRYAG	3 cut(s)	114, 948, 1097
Sfr274I	CTCGAG	1 cut(s)	631
SgrAI	CRCCGGYG	1 cut(s)	28
SinI	GGWCC	5 cut(s)	356, 416, 529, 748, 944
SlaI	CTCGAG	1 cut(s)	631
SmlI	CTYRAG	1 cut(s)	631
SmoI	CTYRAG	1 cut(s)	631
Sse9I	AATT	5 cut(s)	573, 606, 655, 971, 1356
SspMI	CTAG	4 cut(s)	234, 440, 497, 663
StyD4I	CCNGG	3 cut(s)	253, 1143, 1258
StyI	CCWWGG	2 cut(s)	303, 439
TaaI	ACNGT	1 cut(s)	181
TaiI	ACGT	2 cut(s)	270, 648
TaqI	TCGA	3 cut(s)	567, 632, 1005
TasI	AATT	5 cut(s)	573, 606, 655, 971, 1356
TauI	GCSGC	2 cut(s)	73, 100
TfiI	GAWTC	1 cut(s)	230
Tru1I	TTAA	7 cut(s)	183, 449, 804, 836, 996, 1151, 1355
Tru9I	TTAA	7 cut(s)	183, 449, 804, 836, 996, 1151, 1355
TscAI	CASTG	3 cut(s)	160, 268, 617
TseFI	GTSAC	1 cut(s)	787
TseI	GCWGC	7 cut(s)	55, 110, 113, 345, 512, 1025, 1139
Tsp45I	GTSAC	1 cut(s)	787
TspDTI	ATGAA	4 cut(s)	777, 843, 1059, 1181
TspRI	CASTG	3 cut(s)	160, 268, 617
VneI	GTGCAC	1 cut(s)	1284
VpaK11BI	GGWCC	5 cut(s)	356, 416, 529, 748, 944
VspI	ATTAAT	1 cut(s)	996
XagI	CCTNNNNNAGG	1 cut(s)	91
XapI	RAATTY	1 cut(s)	606
XbaI	TCTAGA	1 cut(s)	496
XceI	RCATGY	1 cut(s)	482
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1151
XhoI	CTCGAG	1 cut(s)	631
XmaJI	CCTAGG	1 cut(s)	439
XspI	CTAG	4 cut(s)	234, 440, 497, 663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.