MD03G1154400.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0020368
Physical Location & Seq
Forward (+)
17711659 .. 17712618
960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1154400.v1.1.491

Sequence Viewer

Length: 960 bp
ATGTGTGTTCCTCCACCTCGCGGCCTCATACTATTCTACGCCACTCTACTCGACGCCTGCAGTTCGTCGAAGAACCTCCAAACCCTCACGCAGCTCCACGCTAAAACCATCAAACTCGGCATTTCCCGCCACGACTTCATCCGAACCAAGCTCCTCAGCTCCTACGCAGCCGCTGCCCAACTGAAGCAATGCAACCTGCTTTTCTCCTTCTGTACTCGCCGACCCACCTTCCTCTTCAACACCCTCATCAGAGCCCACTCTTCCCAAGGCCTCTTCTCTCAGTCTCTGTCCATTTTTCTCCGTATGCTTGCCGCCAATAAACCCTGGGACCGCCATACCTTGCCTGCGGTACTCAAATCTTGTGCCGGGTTATCGGCGTTGAGGCTTGGAAAACAAATGCATGGAGCTGTTTTGGTTAATGGGTTTGGCTTCGATTTGGCGAACTCAAATGCGTTGATTAGTATGTATGCGAAGTGTGGTGATTTGGTGGGTGCCCGTAAAGTGTTTGACGGAATGCTGATGAGGAATGAGATTTCTTGGTCGGCAATTATGGCGGGGTATGGGATGCATGGAGTATTTGGCGAGGTGTTTGAGCTGTTTGATAGGATGGTGGAGGCAGGAGAGACGCCGGATGGAATGACTTTCACTACGATTTTGACAGCGTGTAGTCATGGAGGGCTGACAGAGAAGGGGAGGGAGTATTTTGAAATGATGGAGTGGAGGTTCGGGGTGATGCCCGGGCTGGAGCATTATACCTGTATGGTAGATTTGTTGGGTAGGGTGGGACGGGTAGAGGAAGCAGAGGAGTTGGTTTTGGGGATGGCAGTGGAGCCGGATGAGGCATTATGGGGCGCATTGTTGGGTGCTTGTAGGATTCATGGGCAGGTAGAGGTGGCTGAGAGAGTGGAAGCGATGCTTTATGGAAAGCGACTTGGTGTAGCATCTCTGCGAGGCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR

Protein Analysis

320

Amino Acids

35.11

Weight (kDa)

8.35

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 176 - 223 1.7e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0020368)

Species Orthologous Gene IDs
malus_domestica MD00G1049400.v1.1 MD00G1071500.v1.1 MD03G1154400.v1.1
prunus_persica Prupe.6G143600_v2.0.a1
pyrus_communis pycom03g10820 pycom11g14670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 204, 874
AccB1I GGYRCC 1 cut(s) 491
AccII CGCG 1 cut(s) 21
AciI CCGC 7 cut(s) 21, 127, 171, 312, 331, 347, 554
AcuI CTGAAG 1 cut(s) 203
AcyI GRCGYC 2 cut(s) 54, 626
AfaI GTAC 2 cut(s) 214, 351
AfiI CCNNNNNNNGG 1 cut(s) 20
AgsI TTSAA 2 cut(s) 238, 707
AjnI CCWGG 1 cut(s) 323
AloI GAACNNNNNNTCC 2 cut(s) 707, 739
AluBI AGCT 5 cut(s) 94, 151, 159, 407, 595
AluI AGCT 5 cut(s) 94, 151, 159, 407, 595
Alw26I GTCTC 2 cut(s) 288, 617
AlwNI CAGNNNCTG 2 cut(s) 173, 286
Ama87I CYCGRG 1 cut(s) 737
AoxI GGCC 2 cut(s) 22, 268
ApeKI GCWGC 3 cut(s) 91, 167, 173
AspLEI GCGC 1 cut(s) 854
AspS9I GGNCC 1 cut(s) 328
AsuC2I CCSGG 3 cut(s) 367, 738, 739
AsuHPI GGTGA 2 cut(s) 491, 742
AvaI CYCGRG 1 cut(s) 737
AvaII GGWCC 1 cut(s) 328
BaeGI GKGCMC 1 cut(s) 496
BanI GGYRCC 1 cut(s) 491
BanII GRGCYC 1 cut(s) 256
BbvCI CCTCAGC 1 cut(s) 155
BbvI GCAGC 3 cut(s) 103, 160, 179
BccI CCATC 5 cut(s) 116, 601, 626, 706, 814
BciT130I CCWGG 1 cut(s) 325
BcnI CCSGG 3 cut(s) 367, 738, 739
BcoDI GTCTC 2 cut(s) 288, 617
BfmI CTRYAG 1 cut(s) 58
BfuAI ACCTGC 2 cut(s) 204, 874
BisI GCNGC 6 cut(s) 22, 92, 168, 171, 174, 312
BlsI GCNGC 6 cut(s) 23, 93, 169, 172, 175, 313
Bme1390I CCNGG 4 cut(s) 325, 367, 738, 739
Bme18I GGWCC 1 cut(s) 328
BmeT110I CYCGRG 1 cut(s) 737
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 3 cut(s) 329, 493, 831
BmrFI CCNGG 4 cut(s) 325, 367, 738, 739
BmsI GCATC 4 cut(s) 555, 723, 903, 950
BpmI CTGGAG 1 cut(s) 764
Bpu10I CCTNAGC 1 cut(s) 155
BpuMI CCSGG 3 cut(s) 367, 738, 739
BsaHI GRCGYC 2 cut(s) 54, 626
BsaJI CCNNGG 4 cut(s) 265, 323, 324, 737
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 1 cut(s) 20
Bse3DI GCAATG 1 cut(s) 194
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 4 cut(s) 265, 323, 324, 737
BseGI GGATG 6 cut(s) 138, 570, 612, 637, 825, 841
BseLI CCNNNNNNNGG 1 cut(s) 20
BseMI GCAATG 1 cut(s) 194
BseMII CTCAG 3 cut(s) 169, 293, 888
BseRI GAGGAG 2 cut(s) 143, 818
BseSI GKGCMC 1 cut(s) 496
BseXI GCAGC 3 cut(s) 103, 160, 179
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 2 cut(s) 24, 270
BshNI GGYRCC 1 cut(s) 491
BsiHKCI CYCGRG 1 cut(s) 737
BsiSI CCGG 4 cut(s) 366, 629, 738, 833
BslFI GGGAC 2 cut(s) 341, 798
BslI CCNNNNNNNGG 1 cut(s) 20
BsmAI GTCTC 2 cut(s) 288, 617
BsmBI CGTCTC 1 cut(s) 617
BsmFI GGGAC 2 cut(s) 341, 798
BsmI GAATGC 1 cut(s) 519
BsnI GGCC 2 cut(s) 24, 270
BsoBI CYCGRG 1 cut(s) 737
Bsp1286I GDGCHC 2 cut(s) 256, 496
BspACI CCGC 7 cut(s) 21, 127, 171, 312, 331, 347, 554
BspANI GGCC 2 cut(s) 24, 270
BspCNI CTCAG 3 cut(s) 168, 292, 889
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 3 cut(s) 329, 493, 831
BspMAI CTGCAG 1 cut(s) 62
BspMI ACCTGC 2 cut(s) 204, 874
BspT107I GGYRCC 1 cut(s) 491
BsrDI GCAATG 1 cut(s) 194
BssECI CCNNGG 4 cut(s) 265, 323, 324, 737
BssNI GRCGYC 2 cut(s) 54, 626
BssT1I CCWWGG 1 cut(s) 265
Bst2UI CCWGG 1 cut(s) 325
Bst6I CTCTTC 3 cut(s) 239, 265, 278
BstACI GRCGYC 2 cut(s) 54, 626
BstAPI GCANNNNNTGC 1 cut(s) 173
BstC8I GCNNGC 3 cut(s) 58, 309, 345
BstDEI CTNAG 3 cut(s) 155, 279, 897
BstF5I GGATG 6 cut(s) 138, 570, 612, 637, 825, 841
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 1 cut(s) 854
BstMAI GTCTC 2 cut(s) 288, 617
BstMWI GCNNNNNNNGC 3 cut(s) 126, 173, 551
BstNI CCWGG 1 cut(s) 325
BstSCI CCNGG 4 cut(s) 323, 365, 736, 737
BstSFI CTRYAG 1 cut(s) 58
BstSLI GKGCMC 1 cut(s) 496
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 3 cut(s) 103, 160, 179
BsuRI GGCC 2 cut(s) 24, 270
BtgZI GCGATG 1 cut(s) 926
BtsCI GGATG 6 cut(s) 138, 570, 612, 637, 825, 841
BtsI GCAGTG 1 cut(s) 831
BtsIMutI CAGTG 1 cut(s) 831
BveI ACCTGC 2 cut(s) 204, 874
Cac8I GCNNGC 3 cut(s) 58, 309, 345
CaiI CAGNNNCTG 2 cut(s) 173, 286
CfoI GCGC 1 cut(s) 854
Cfr13I GGNCC 1 cut(s) 328
Cfr9I CCCGGG 1 cut(s) 737
CseI GACGC 2 cut(s) 62, 634
Csp6I GTAC 2 cut(s) 213, 350
CviAII CATG 4 cut(s) 401, 569, 671, 878
CviQI GTAC 2 cut(s) 213, 350
DdeI CTNAG 3 cut(s) 155, 279, 897
Eam1104I CTCTTC 3 cut(s) 239, 265, 278
EarI CTCTTC 3 cut(s) 239, 265, 278
Eco130I CCWWGG 1 cut(s) 265
Eco147I AGGCCT 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 256
Eco47I GGWCC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 203
Eco88I CYCGRG 1 cut(s) 737
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 265
EcoT22I ATGCAT 2 cut(s) 402, 570
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 617
FaeI CATG 4 cut(s) 404, 572, 674, 881
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FaqI GGGAC 2 cut(s) 341, 798
FatI CATG 4 cut(s) 400, 568, 670, 877
FauI CCCGC 2 cut(s) 134, 547
Fnu4HI GCNGC 6 cut(s) 22, 92, 168, 171, 174, 312
FokI GGATG 6 cut(s) 125, 577, 619, 644, 832, 848
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 6 cut(s) 22, 92, 168, 171, 174, 312
GlaI GCGC 1 cut(s) 853
GluI GCNGC 6 cut(s) 22, 92, 168, 171, 174, 312
GsuI CTGGAG 1 cut(s) 764
HaeIII GGCC 2 cut(s) 24, 270
HapII CCGG 4 cut(s) 366, 629, 738, 833
HgaI GACGC 2 cut(s) 62, 634
HhaI GCGC 1 cut(s) 854
Hin1I GRCGYC 2 cut(s) 54, 626
Hin1II CATG 4 cut(s) 404, 572, 674, 881
Hin6I GCGC 1 cut(s) 852
HinP1I GCGC 1 cut(s) 852
HinfI GANTC 1 cut(s) 874
HpaII CCGG 4 cut(s) 366, 629, 738, 833
HphI GGTGA 2 cut(s) 491, 742
Hpy188I TCNGA 2 cut(s) 143, 251
Hpy99I CGWCG 2 cut(s) 56, 70
HpyAV CCTTC 3 cut(s) 217, 238, 682
HpyCH4V TGCA 4 cut(s) 60, 192, 400, 568
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 173, 551
HpyF3I CTNAG 3 cut(s) 155, 279, 897
Hsp92I GRCGYC 2 cut(s) 54, 626
Hsp92II CATG 4 cut(s) 404, 572, 674, 881
HspAI GCGC 1 cut(s) 852
LmnI GCTCC 6 cut(s) 99, 156, 164, 404, 745, 829
Lsp1109I GCAGC 3 cut(s) 103, 160, 179
LweI GCATC 4 cut(s) 555, 723, 903, 950
MboII GAAGA 4 cut(s) 82, 226, 252, 265
MhlI GDGCHC 2 cut(s) 256, 496
MluCI AATT 1 cut(s) 546
Mph1103I ATGCAT 2 cut(s) 402, 570
MseI TTAA 2 cut(s) 417, 958
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 4 cut(s) 366, 629, 738, 833
MspR9I CCNGG 4 cut(s) 325, 367, 738, 739
Mva1269I GAATGC 1 cut(s) 519
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 126, 173, 551
NciI CCSGG 3 cut(s) 367, 738, 739
NlaIII CATG 4 cut(s) 404, 572, 674, 881
NlaIV GGNNCC 3 cut(s) 329, 493, 831
NmeAIII GCCGAG 1 cut(s) 96
NsiI ATGCAT 2 cut(s) 402, 570
PasI CCCWGGG 1 cut(s) 324
PceI AGGCCT 1 cut(s) 270
PctI GAATGC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 874
PkrI GCNGC 6 cut(s) 23, 93, 169, 172, 175, 313
Psp6I CCWGG 1 cut(s) 323
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 3 cut(s) 329, 493, 831
PspPI GGNCC 1 cut(s) 328
PstI CTGCAG 1 cut(s) 62
PstNI CAGNNNCTG 2 cut(s) 173, 286
RsaI GTAC 2 cut(s) 214, 351
RsaNI GTAC 2 cut(s) 213, 350
SaqAI TTAA 2 cut(s) 417, 958
SatI GCNGC 6 cut(s) 22, 92, 168, 171, 174, 312
Sau96I GGNCC 1 cut(s) 328
ScrFI CCNGG 4 cut(s) 325, 367, 738, 739
SduI GDGCHC 2 cut(s) 256, 496
SfaNI GCATC 4 cut(s) 555, 723, 903, 950
SfcI CTRYAG 1 cut(s) 58
SinI GGWCC 1 cut(s) 328
SmaI CCCGGG 1 cut(s) 739
SrfI GCCCGGGC 1 cut(s) 739
Sse9I AATT 1 cut(s) 546
SseBI AGGCCT 1 cut(s) 270
SsiI CCGC 7 cut(s) 21, 127, 171, 312, 331, 347, 554
StuI AGGCCT 1 cut(s) 270
StyD4I CCNGG 4 cut(s) 323, 365, 736, 737
StyI CCWWGG 1 cut(s) 265
TaqI TCGA 3 cut(s) 51, 68, 432
TasI AATT 1 cut(s) 546
TatI WGTACW 1 cut(s) 212
TauI GCSGC 3 cut(s) 24, 173, 314
TfiI GAWTC 1 cut(s) 874
Tru1I TTAA 2 cut(s) 417, 958
Tru9I TTAA 2 cut(s) 417, 958
TscAI CASTG 1 cut(s) 831
TseI GCWGC 3 cut(s) 91, 167, 173
TspDTI ATGAA 2 cut(s) 127, 866
TspGWI ACGGA 2 cut(s) 290, 525
TspMI CCCGGG 1 cut(s) 737
TspRI CASTG 1 cut(s) 831
VpaK11BI GGWCC 1 cut(s) 328
XmaI CCCGGG 1 cut(s) 737
Zsp2I ATGCAT 2 cut(s) 402, 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.