MD03G1185900.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011543
Physical Location & Seq
Reverse (-)
25338234 .. 25341703
3470 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1185900.v1.1.491

Sequence Viewer

Length: 2484 bp
ATGGAAATCTTTACAATGCTTTTCGTTTTATCATCTTTCCTCTTCTCTCCAAGCATCTCCGCTGCAAAAGATATCATCACTCCGACAGTATCGCTCAGAGATGGAGAGTCCCTTGTTTCCTCCGGCGGAACATTCGAACTAGGATTCTTTAGCCCAGGTAATTCGAAGAATCGATACGTTGGAATATGGTACAATGGAATATCTCCTCAAATTGTTGTGTGGGTGGCCAACAGAGAAACTCCACTTACTGATCATTCTGGAGCTTTGAACATCACTGATGGAGGAGTTCTTGTTCTTTTCAGTGACAAAAATGGCAGTCTGTGGTCATCTAATTTAACTTCAAGAACCCTAAGGAGCCCGGTTGCTCAGCTCCTGGAGTCCGGCAACCTTGTTGTGAAAGACGGAAATGCTGACAACTCGGAGGGCAACCTTGTGTGGCAGAGTTTTGATTATCCAGGCAACATCTTACTACCAGGAATGAAGCTTGGATGGAACTTAGCGACAGGTAAAAACAGGTTTTTGTCTTCGTGGAAAGCCGCAGAGGATCCATCTCCAGGTGACTTCTCGTATTCCATAGATCATAGCGGATACCCCCAACTAGTCCTTCGGAAAGGTCGTGTTCCACACTATAGGTTGGGGTCATGGAATGGCCTTGGTTTTACTGGGAGTCCTGAACTTAGTCCTAATGAAGCATTTAAAATCGAATTTGTGTCCAATGATAGTGAGGCCTATTACAAATTCGACCTCATGCGCGATTCGATGCATTCGAGATTGGTACTCAACCTGTCTGGTGGTTTGCAGCGGTTCTTGTGGATTGATAAAACACATAGCGGGAACATGATTTACTCTGCTCCAGCAGATGGTTGTGACACTTACGATTTGTGTGGTGCATTCGCTTTCTGCAAGCTTGATTCTGTTCCTTTATGTTCATGCTTGCAAGGTTTTGTACCAAAATCTCCAAGAGACTACTGGACTATGAAAAACACGTCTTCAGGATGTCTTCGTAAAAGTCCATTGAATTGTACGAATCAAGATGAATTTCAGAAGTTCATACGGGTGAAATTGCCAGACACATCTTCGTCACGGATCAATAATACCATGTCTCTCGAGGAATGTAAGAAAATGTGTTTGACAAACTGCTCTTGCACAGCATATGCAAATTTGGATGTTAGGGGAGGAGGAACTGGCTGTTTGCTTTGGTTCGGCGACCTCATTGATATAAAAGAATTCGACTCTGTTGGGCAAGATCTTTTTGTCCGGATGCCCCCCTCCGAATTAGATGGCGCTAAGAGAAGCAAGCACTCTGGTGTACAAAGGAAAGTGGCAATCATAGTCTTCTCTGGGGCAGTAGTCATGGGAATGCTAATACTAGGATTGGTTTTCTACATACAGAAGAAGAAATCGAAAGCGCGAGGAAACATGAAAAACATACGTGAAAAGAACTTTGGTTTCGAAGGTGGAAGCCAGGACATGGAGTTGATAACATTTGATTTGGCCACTGTATCTAGAGCCACCGATAACTTCTCAGACAATAACAAGCTGGGACAAGGTGGTTTCGGACCAGTGTACAAGGGAATCCTGAAGGAAGGGCAAAATATAGCAGTGAAAAGGCTTTCGAAATATTCTGGGCAAGGAACCAAAGAATTCATGAATGAAGTAGTATTAATTGCTAAACTTCAACACCGAAATCTTGTAAAGCTTCTTGGTTGCTGTATTGAAGGGGATGAAAAGATGTTGATCTATGAATATATGCCAAACAAAAGCTTGGACTACTTCATATTTGACGATACAAGAAGTAAATTCCTCAATTGGGATCAGCTCATCAACATTATCGGAGGAATTGCTCGAGGACTTCTCTATCTTCATCAAGATTCTAGACTGAGGATCATCCATAGGGATCTCAAAACCAGTAACGTTTTACTAGATAAAGATATGAACCCAAAAATATCTGATTTTGGCACGGCTAGAGAATTTGGGGGAGATCAAACTGAGGAAAATACTAATAGAGTGGTTGGAACATATGGCTACATGTCTCCTGAGTACGTGGTCGATGGACTCTTCTCAGTCAAATCGGATGTTTATAGCTTCGGTGTGATGGTGCTGGAGATACTTAGCGGGAAGAAGAACAGGGGATTTCATCATCCAGACCACCAGCTTAACCTTCTAGGACATGCTTGGATACTTTGGATTGAAGGAAGGCCAACAGAAATAATGGATGAGAAGTTAGATGGATCGTGTGCGCCACCAGAAGTGTCACGATGCATTCATATAGCTCTACTGTGTGTGCAGCAACAACCAGAAGATAGGCCAAACATGGCATCTGTGGTCCTAATGTTGGGTGGGGAGGGTTCATTGCCTATACCCAAGCAACCTGGTTTCTTTACTGAAAGGAACTCGGTTGAAGCAAATGCTTCTTCGCCAACCAAGTACGAATCCCATTCCATAAATGAGATGAGTCTAACGCTGTTAGAAGCAAGGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

828

Amino Acids

91.86

Weight (kDa)

6.46

Isoelectric Point (pI)

48.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 3.3e-35 D-mannose binding lectin
S_locus_glycop PF00954 210 - 319 2.4e-28 S-locus glycoprotein domain
PAN_2 PF08276 341 - 407 6.1e-22 PAN-like domain
Pkinase PF00069 509 - 775 5.8e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 511 - 776 1.2e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 783 - 826 3.9e-10 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 860, 1471
AccII CGCG 2 cut(s) 753, 1411
AccIII TCCGGA 1 cut(s) 1257
AciI CCGC 7 cut(s) 60, 126, 537, 585, 802, 831, 2115
AclI AACGTT 1 cut(s) 1914
AclWI GGATC 7 cut(s) 539, 552, 1094, 1821, 1892, 1905, 2239
AcoI YGGCCR 2 cut(s) 225, 1494
AcsI RAATTY 8 cut(s) 704, 737, 1037, 1159, 1226, 1643, 1799, 1970
AcuI CTGAAG 2 cut(s) 975, 1601
AfaI GTAC 8 cut(s) 191, 777, 948, 1024, 1311, 1568, 2042, 2429
AfiI CCNNNNNNNGG 5 cut(s) 554, 860, 1471, 1810, 2335
AflIII ACRYGT 2 cut(s) 984, 2028
AgsI TTSAA 7 cut(s) 268, 342, 1018, 1679, 1718, 2192, 2402
AhlI ACTAGT 1 cut(s) 598
AjiI CACGTC 1 cut(s) 987
AjnI CCWGG 7 cut(s) 154, 372, 454, 472, 553, 1464, 2371
AjuI GAANNNNNNNTTGG 4 cut(s) 588, 620, 1428, 1460
AleI CACNNNNGTG 1 cut(s) 1305
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
Alw26I GTCTC 3 cut(s) 957, 1107, 2037
AlwI GGATC 7 cut(s) 539, 552, 1094, 1821, 1892, 1905, 2239
AlwNI CAGNNNCTG 1 cut(s) 373
Ama87I CYCGRG 2 cut(s) 1106, 1845
Aor13HI TCCGGA 1 cut(s) 1257
AoxI GGCC 6 cut(s) 225, 649, 726, 1494, 2198, 2306
ApeKI GCWGC 3 cut(s) 62, 799, 2287
ApoI RAATTY 8 cut(s) 704, 737, 1037, 1159, 1226, 1643, 1799, 1970
AseI ATTAAT 1 cut(s) 1664
AspLEI GCGC 4 cut(s) 753, 1286, 1411, 2242
AspS9I GGNCC 2 cut(s) 1559, 2326
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 2 cut(s) 569, 1069
AsuII TTCGAA 4 cut(s) 135, 164, 1452, 1616
AvaI CYCGRG 2 cut(s) 1106, 1845
AvaII GGWCC 2 cut(s) 1559, 2326
AxyI CCTNAGG 1 cut(s) 350
BalI TGGCCA 2 cut(s) 227, 1496
BamHI GGATCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 359
BarI GAAGNNNNNNTAC 2 cut(s) 158, 190
BbsI GAAGAC 4 cut(s) 516, 981, 992, 1327
BbvI GCAGC 3 cut(s) 49, 811, 2299
BccI CCATC 9 cut(s) 95, 272, 483, 556, 854, 1274, 2045, 2089, 2222
BceAI ACGGC 1 cut(s) 1977
BciT130I CCWGG 7 cut(s) 156, 374, 456, 474, 555, 1466, 2373
BciVI GTATCC 2 cut(s) 581, 2172
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 3 cut(s) 957, 1107, 2037
BcuI ACTAGT 1 cut(s) 598
BfaI CTAG 8 cut(s) 140, 599, 1370, 1506, 1875, 1922, 1965, 2165
BfmI CTRYAG 1 cut(s) 628
BfoI RGCGCY 1 cut(s) 1287
BfuI GTATCC 2 cut(s) 581, 2172
BglII AGATCT 1 cut(s) 1246
BisI GCNGC 4 cut(s) 63, 537, 800, 2288
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 4 cut(s) 64, 538, 801, 2289
Bme1390I CCNGG 8 cut(s) 156, 359, 374, 456, 474, 555, 1466, 2373
Bme18I GGWCC 2 cut(s) 1559, 2326
BmeT110I CYCGRG 2 cut(s) 1106, 1845
BmgBI CACGTC 1 cut(s) 987
BmgT120I GGNCC 2 cut(s) 1559, 2326
BmiI GGNNCC 3 cut(s) 356, 546, 1636
BmrFI CCNGG 8 cut(s) 156, 359, 374, 456, 474, 555, 1466, 2373
BmrI ACTGGG 1 cut(s) 672
BmsI GCATC 5 cut(s) 63, 750, 1251, 2249, 2327
BmuI ACTGGG 1 cut(s) 672
BpiI GAAGAC 4 cut(s) 516, 981, 992, 1327
BplI GAGNNNNNCTC 2 cut(s) 1839, 1871
BpmI CTGGAG 5 cut(s) 279, 395, 537, 837, 2123
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 4 cut(s) 135, 164, 1452, 1616
BpuMI CCSGG 1 cut(s) 359
Bsa29I ATCGAT 1 cut(s) 172
BsaAI YACGTR 2 cut(s) 1433, 2044
BsaBI GATNNNNATC 1 cut(s) 1736
BsaJI CCNNGG 2 cut(s) 154, 652
BsaWI WCCGGW 1 cut(s) 1257
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 5 cut(s) 554, 860, 1471, 1810, 2335
Bse1I ACTGG 5 cut(s) 667, 974, 1189, 1562, 1908
Bse21I CCTNAGG 1 cut(s) 350
Bse3DI GCAATG 1 cut(s) 2351
Bse8I GATNNNNATC 1 cut(s) 1736
BseAI TCCGGA 1 cut(s) 1257
BseBI CCWGG 7 cut(s) 156, 374, 456, 474, 555, 1466, 2373
BseCI ATCGAT 1 cut(s) 172
BseDI CCNNGG 2 cut(s) 154, 652
BseGI GGATG 9 cut(s) 494, 1001, 1171, 1266, 1729, 1887, 2080, 2140, 2221
BseJI GATNNNNATC 1 cut(s) 1736
BseLI CCNNNNNNNGG 5 cut(s) 554, 860, 1471, 1810, 2335
BseMI GCAATG 1 cut(s) 2351
BseMII CTCAG 7 cut(s) 109, 380, 1539, 1871, 1980, 2028, 2076
BseNI ACTGG 5 cut(s) 667, 974, 1189, 1562, 1908
BseRI GAGGAG 3 cut(s) 195, 297, 1191
BseXI GCAGC 3 cut(s) 49, 811, 2299
BseYI CCCAGC 1 cut(s) 1540
BsgI GTGCAG 1 cut(s) 2306
Bsh1236I CGCG 2 cut(s) 753, 1411
BshFI GGCC 6 cut(s) 227, 651, 728, 1496, 2200, 2308
BshVI ATCGAT 1 cut(s) 172
BsiHKCI CYCGRG 2 cut(s) 1106, 1845
BsiSI CCGG 4 cut(s) 123, 359, 381, 1258
BslFI GGGAC 2 cut(s) 94, 1557
BslI CCNNNNNNNGG 5 cut(s) 554, 860, 1471, 1810, 2335
BsmAI GTCTC 3 cut(s) 957, 1107, 2037
BsmFI GGGAC 2 cut(s) 94, 1557
BsmI GAATGC 4 cut(s) 763, 890, 1365, 2262
BsnI GGCC 6 cut(s) 227, 651, 728, 1496, 2200, 2308
BsoBI CYCGRG 2 cut(s) 1106, 1845
Bsp119I TTCGAA 4 cut(s) 135, 164, 1452, 1616
Bsp1286I GDGCHC 1 cut(s) 359
Bsp13I TCCGGA 1 cut(s) 1257
Bsp1407I TGTACA 2 cut(s) 1309, 1566
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 7 cut(s) 60, 126, 537, 585, 802, 831, 2115
BspANI GGCC 6 cut(s) 227, 651, 728, 1496, 2200, 2308
BspCNI CTCAG 7 cut(s) 108, 379, 1538, 1872, 1981, 2029, 2075
BspDI ATCGAT 1 cut(s) 172
BspEI TCCGGA 1 cut(s) 1257
BspFNI CGCG 2 cut(s) 753, 1411
BspHI TCATGA 1 cut(s) 1647
BspLI GGNNCC 3 cut(s) 356, 546, 1636
BspPI GGATC 7 cut(s) 539, 552, 1094, 1821, 1892, 1905, 2239
BspT104I TTCGAA 4 cut(s) 135, 164, 1452, 1616
BsrDI GCAATG 1 cut(s) 2351
BsrGI TGTACA 2 cut(s) 1309, 1566
BsrI ACTGG 5 cut(s) 667, 974, 1189, 1562, 1908
BssECI CCNNGG 2 cut(s) 154, 652
BssT1I CCWWGG 1 cut(s) 652
Bst2UI CCWGG 7 cut(s) 156, 374, 456, 474, 555, 1466, 2373
Bst4CI ACNGT 3 cut(s) 88, 1501, 2280
Bst6I CTCTTC 2 cut(s) 47, 2063
BstAUI TGTACA 2 cut(s) 1309, 1566
BstBAI YACGTR 2 cut(s) 1433, 2044
BstBI TTCGAA 4 cut(s) 135, 164, 1452, 1616
BstC8I GCNNGC 3 cut(s) 905, 935, 1298
BstF5I GGATG 9 cut(s) 494, 1001, 1171, 1266, 1729, 1887, 2080, 2140, 2221
BstFNI CGCG 2 cut(s) 753, 1411
BstH2I RGCGCY 1 cut(s) 1287
BstHHI GCGC 4 cut(s) 753, 1286, 1411, 2242
BstMAI GTCTC 3 cut(s) 957, 1107, 2037
BstNI CCWGG 7 cut(s) 156, 374, 456, 474, 555, 1466, 2373
BstNSI RCATGY 2 cut(s) 2032, 2174
BstSCI CCNGG 8 cut(s) 154, 357, 372, 454, 472, 553, 1464, 2371
BstSFI CTRYAG 1 cut(s) 628
BstUI CGCG 2 cut(s) 753, 1411
BstV1I GCAGC 3 cut(s) 49, 811, 2299
BstV2I GAAGAC 4 cut(s) 516, 981, 992, 1327
BstX2I RGATCY 3 cut(s) 544, 1246, 1897
BstYI RGATCY 3 cut(s) 544, 1246, 1897
Bsu15I ATCGAT 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 350
BsuI GTATCC 2 cut(s) 581, 2172
BsuRI GGCC 6 cut(s) 227, 651, 728, 1496, 2200, 2308
BsuTUI ATCGAT 1 cut(s) 172
BtrI CACGTC 1 cut(s) 987
BtsCI GGATG 9 cut(s) 494, 1001, 1171, 1266, 1729, 1887, 2080, 2140, 2221
BtsI GCAGTG 1 cut(s) 1608
BtsIMutI CAGTG 5 cut(s) 273, 307, 1497, 1569, 1608
Cac8I GCNNGC 3 cut(s) 905, 935, 1298
CaiI CAGNNNCTG 1 cut(s) 373
CciI TCATGA 1 cut(s) 1647
CfoI GCGC 4 cut(s) 753, 1286, 1411, 2242
Cfr13I GGNCC 2 cut(s) 1559, 2326
ClaI ATCGAT 1 cut(s) 172
CsiI ACCWGGT 1 cut(s) 2371
Csp6I GTAC 8 cut(s) 190, 776, 947, 1023, 1310, 1567, 2041, 2428
CviQI GTAC 8 cut(s) 190, 776, 947, 1023, 1310, 1567, 2041, 2428
DraI TTTAAA 1 cut(s) 697
EaeI YGGCCR 2 cut(s) 225, 1494
Eam1104I CTCTTC 2 cut(s) 47, 2063
EarI CTCTTC 2 cut(s) 47, 2063
EciI GGCGGA 1 cut(s) 141
Eco130I CCWWGG 1 cut(s) 652
Eco147I AGGCCT 1 cut(s) 728
Eco24I GRGCYC 1 cut(s) 359
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 1559, 2326
Eco57I CTGAAG 2 cut(s) 975, 1601
Eco81I CCTNAGG 1 cut(s) 350
Eco88I CYCGRG 2 cut(s) 1106, 1845
EcoRI GAATTC 2 cut(s) 1226, 1643
EcoRII CCWGG 7 cut(s) 154, 372, 454, 472, 553, 1464, 2371
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 1 cut(s) 652
EcoT22I ATGCAT 2 cut(s) 765, 2264
EcoT38I GRGCYC 1 cut(s) 359
ErhI CCWWGG 1 cut(s) 652
FaqI GGGAC 2 cut(s) 94, 1557
FauI CCCGC 2 cut(s) 824, 2108
FauNDI CATATG 2 cut(s) 1153, 2020
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 4 cut(s) 63, 537, 800, 2288
FokI GGATG 9 cut(s) 501, 1008, 1178, 1273, 1736, 1874, 2087, 2127, 2228
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 4 cut(s) 63, 537, 800, 2288
FspBI CTAG 8 cut(s) 140, 599, 1370, 1506, 1875, 1922, 1965, 2165
GlaI GCGC 4 cut(s) 752, 1285, 1410, 2241
GluI GCNGC 4 cut(s) 63, 537, 800, 2288
GsaI CCCAGC 1 cut(s) 1544
GsuI CTGGAG 5 cut(s) 279, 395, 537, 837, 2123
HaeII RGCGCY 1 cut(s) 1287
HaeIII GGCC 6 cut(s) 227, 651, 728, 1496, 2200, 2308
HapII CCGG 4 cut(s) 123, 359, 381, 1258
HhaI GCGC 4 cut(s) 753, 1286, 1411, 2242
Hin6I GCGC 4 cut(s) 751, 1284, 1409, 2240
HinP1I GCGC 4 cut(s) 751, 1284, 1409, 2240
HindIII AAGCTT 4 cut(s) 482, 905, 1697, 1762
HpaII CCGG 4 cut(s) 123, 359, 381, 1258
HphI GGTGA 2 cut(s) 569, 1069
Hpy166II GTNNAC 2 cut(s) 1310, 1567
Hpy8I GTNNAC 2 cut(s) 1310, 1567
HpyAV CCTTC 8 cut(s) 614, 1448, 1576, 1580, 1712, 2171, 2186, 2190
HpyCH4III ACNGT 3 cut(s) 88, 1501, 2280
HpyCH4IV ACGT 5 cut(s) 177, 986, 1432, 1914, 2043
HpySE526I ACGT 5 cut(s) 177, 986, 1432, 1914, 2043
HspAI GCGC 4 cut(s) 751, 1284, 1409, 2240
Kpn2I TCCGGA 1 cut(s) 1257
Ksp22I TGATCA 1 cut(s) 250
LmnI GCTCC 4 cut(s) 260, 354, 375, 856
Lsp1109I GCAGC 3 cut(s) 49, 811, 2299
LweI GCATC 5 cut(s) 63, 750, 1251, 2249, 2327
MabI ACCWGGT 1 cut(s) 2371
MaeI CTAG 8 cut(s) 140, 599, 1370, 1506, 1875, 1922, 1965, 2165
MaeII ACGT 5 cut(s) 177, 986, 1432, 1914, 2043
MaeIII GTNAC 6 cut(s) 302, 557, 866, 1080, 1910, 2253
MfeI CAATTG 1 cut(s) 1807
MflI RGATCY 3 cut(s) 544, 1246, 1897
MhlI GDGCHC 1 cut(s) 359
MlsI TGGCCA 2 cut(s) 227, 1496
MluNI TGGCCA 2 cut(s) 227, 1496
MlyI GAGTC 6 cut(s) 116, 386, 676, 1226, 2049, 2464
MmeI TCCRAC 3 cut(s) 107, 160, 1993
Mox20I TGGCCA 2 cut(s) 227, 1496
Mph1103I ATGCAT 2 cut(s) 765, 2264
MroI TCCGGA 1 cut(s) 1257
MscI TGGCCA 2 cut(s) 227, 1496
MseI TTAA 5 cut(s) 335, 696, 1664, 2157, 2482
MslI CAYNNNNRTG 3 cut(s) 1055, 1305, 1358
Msp20I TGGCCA 2 cut(s) 227, 1496
MspA1I CMGCKG 2 cut(s) 62, 802
MspI CCGG 4 cut(s) 123, 359, 381, 1258
MspR9I CCNGG 8 cut(s) 156, 359, 374, 456, 474, 555, 1466, 2373
MunI CAATTG 1 cut(s) 1807
Mva1269I GAATGC 4 cut(s) 763, 890, 1365, 2262
MvaI CCWGG 7 cut(s) 156, 374, 456, 474, 555, 1466, 2373
MvnI CGCG 2 cut(s) 753, 1411
NciI CCSGG 1 cut(s) 359
NdeI CATATG 2 cut(s) 1153, 2020
NlaIV GGNNCC 3 cut(s) 356, 546, 1636
NmuCI GTSAC 5 cut(s) 302, 557, 866, 1080, 2253
NsiI ATGCAT 2 cut(s) 765, 2264
NspI RCATGY 2 cut(s) 2032, 2174
NspV TTCGAA 4 cut(s) 135, 164, 1452, 1616
OliI CACNNNNGTG 1 cut(s) 1305
PaeR7I CTCGAG 2 cut(s) 1106, 1845
PagI TCATGA 1 cut(s) 1647
PceI AGGCCT 1 cut(s) 728
PciI ACATGT 1 cut(s) 2028
PcsI WCGNNNNNNNCGW 2 cut(s) 613, 764
PctI GAATGC 4 cut(s) 763, 890, 1365, 2262
PfeI GAWTC 8 cut(s) 144, 169, 755, 911, 1027, 1575, 1871, 2432
PflMI CCANNNNNTGG 2 cut(s) 860, 1471
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 4 cut(s) 64, 538, 801, 2289
PleI GAGTC 6 cut(s) 115, 385, 675, 1226, 2049, 2463
PpsI GAGTC 6 cut(s) 115, 385, 675, 1226, 2049, 2463
Ppu21I YACGTR 2 cut(s) 1433, 2044
PscI ACATGT 1 cut(s) 2028
PshBI ATTAAT 1 cut(s) 1664
Psp1406I AACGTT 1 cut(s) 1914
Psp6I CCWGG 7 cut(s) 154, 372, 454, 472, 553, 1464, 2371
PspFI CCCAGC 1 cut(s) 1540
PspGI CCWGG 7 cut(s) 154, 372, 454, 472, 553, 1464, 2371
PspN4I GGNNCC 3 cut(s) 356, 546, 1636
PspPI GGNCC 2 cut(s) 1559, 2326
PspXI VCTCGAGB 1 cut(s) 1845
PsrI GAACNNNNNNTAC 2 cut(s) 827, 859
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 3 cut(s) 544, 1246, 1897
RsaI GTAC 8 cut(s) 191, 777, 948, 1024, 1311, 1568, 2042, 2429
RsaNI GTAC 8 cut(s) 190, 776, 947, 1023, 1310, 1567, 2041, 2428
RseI CAYNNNNRTG 3 cut(s) 1055, 1305, 1358
SaqAI TTAA 5 cut(s) 335, 696, 1664, 2157, 2482
SatI GCNGC 4 cut(s) 63, 537, 800, 2288
Sau96I GGNCC 2 cut(s) 1559, 2326
SchI GAGTC 6 cut(s) 116, 386, 676, 1226, 2049, 2464
ScrFI CCNGG 8 cut(s) 156, 359, 374, 456, 474, 555, 1466, 2373
SduI GDGCHC 1 cut(s) 359
SexAI ACCWGGT 1 cut(s) 2371
SfaNI GCATC 5 cut(s) 63, 750, 1251, 2249, 2327
SfcI CTRYAG 1 cut(s) 628
Sfr274I CTCGAG 2 cut(s) 1106, 1845
SfuI TTCGAA 4 cut(s) 135, 164, 1452, 1616
SinI GGWCC 2 cut(s) 1559, 2326
SlaI CTCGAG 2 cut(s) 1106, 1845
SmiMI CAYNNNNRTG 3 cut(s) 1055, 1305, 1358
SmlI CTYRAG 2 cut(s) 1106, 1845
SmoI CTYRAG 2 cut(s) 1106, 1845
SpeI ACTAGT 1 cut(s) 598
SseBI AGGCCT 1 cut(s) 728
SsiI CCGC 7 cut(s) 60, 126, 537, 585, 802, 831, 2115
SspI AATATT 1 cut(s) 1622
SspMI CTAG 8 cut(s) 140, 599, 1370, 1506, 1875, 1922, 1965, 2165
StuI AGGCCT 1 cut(s) 728
StyD4I CCNGG 8 cut(s) 154, 357, 372, 454, 472, 553, 1464, 2371
StyI CCWWGG 1 cut(s) 652
TaaI ACNGT 3 cut(s) 88, 1501, 2280
TaiI ACGT 5 cut(s) 180, 989, 1435, 1917, 2046
TatI WGTACW 2 cut(s) 1309, 1566
TauI GCSGC 1 cut(s) 539
TfiI GAWTC 8 cut(s) 144, 169, 755, 911, 1027, 1575, 1871, 2432
Tru1I TTAA 5 cut(s) 335, 696, 1664, 2157, 2482
Tru9I TTAA 5 cut(s) 335, 696, 1664, 2157, 2482
TscAI CASTG 5 cut(s) 280, 307, 1504, 1569, 1608
TseFI GTSAC 5 cut(s) 302, 557, 866, 1080, 2253
TseI GCWGC 3 cut(s) 62, 799, 2287
Tsp45I GTSAC 5 cut(s) 302, 557, 866, 1080, 2253
TspGWI ACGGA 2 cut(s) 417, 1099
TspRI CASTG 5 cut(s) 280, 307, 1504, 1569, 1608
Van91I CCANNNNNTGG 2 cut(s) 860, 1471
VpaK11BI GGWCC 2 cut(s) 1559, 2326
VspI ATTAAT 1 cut(s) 1664
XapI RAATTY 8 cut(s) 704, 737, 1037, 1159, 1226, 1643, 1799, 1970
XbaI TCTAGA 2 cut(s) 1505, 1874
XceI RCATGY 2 cut(s) 2032, 2174
XcmI CCANNNNNNNNNTGG 1 cut(s) 966
XhoI CTCGAG 2 cut(s) 1106, 1845
XspI CTAG 8 cut(s) 140, 599, 1370, 1506, 1875, 1922, 1965, 2165
Zsp2I ATGCAT 2 cut(s) 765, 2264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.