Rh5BG250400

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011543
Physical Location & Seq
Forward (+)
30745115 .. 30748862
3748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG250400.1

Sequence Viewer

Length: 2475 bp
ATGGAAATCTTTCCAATGCTTGTTGTTGTCTCAGCATTTCTCTTCTCCCTTCTAAGTACCTCCACTACCAGTATACAAGACATCATTACCATTAATCCAAGAGTATCAATCAAAGATGGAGGGAGTACCTTAGTTTCAGCTAAAGGAACAGCTGGAGGAATCTTCAAATTGGGATTCTTTAGCCCAGGTAATTCGAAGAATCGATACGTGGGAATATGGTACAATGACATATCTCCTCAGAAAGTTGTATGGGTGTGCAACAAAGAAACTCCCCTTACTGATCACTCGGGAACTTTGAATCTCACTAGCCAAGGAGTTCTCGTTCTGTTCAATGGCACAAATAGTATTGTCTGGTCATCATCCAACAAGACTTCAAGCAACATAAGTAACCCAGTTGCTCAACTCTTGGAGTCGGGCAATTTCGTTGTGAAAGATGGATCAAATGAGGACTCAAAAGTTCTGTGGCAGAGCTTTGACTATCCAAGTGACACCTTACTACCAGGAATGAAGATCGGATGGGACTTAAAAACTGGCCTAAACAGGTTTCTGTCATCTTGGAAAGAAGCAGATGATCCTGCTCCAGGACAATTTTCATTTTCATTAGATCGTAGTGGGTATCCCCAACTAGTTCTTAGGAATGGTTCCATAGCACATTACAGGTTGGGGTCATGGAATGGCCTTGGCTTCACAGGAAGTCCTCAACTTAGATCTCAGAACCAGTTTTTCAAACTTGACTTTGTGTCAAATGAGAATGAAGTATACTACAAATATGAGCTCCTGAGCACTTCACTTGAATCAAGATTAGTGCTCAACAGGTCAGGCAATTTGCAACGCTTCATGTGGAGTGGCACAAAAAATATCAATCGCGTCGTTTACTCAGCTCCAGCAGATCAGTGTGACGCTTATAATGTGTGTGGTGCATTTGCTCTCTGCAGTGTTGATTCTCCTCAATTATGTGCATGCTTACAAGGTTTTGCACCAAATTCTTCAACGTCCACCAGGTGTCTTCGTAGAACTCCATTGAGTTGCAATGATTACCAAGATGGCTTCAAGAAGTTCACAAATGTGAAATTGCCGGACACATCTTTCTCCTGGTTTAACTTGACAATGAGCCTTGAGGACTGTAAGAAAATTTGTTTGAAGAACTGCAATTGCACTGCTTATACAAATGTGGATCTAAGGGAAGGAGGAAGTGGCTGTTTGCTCTGGTTTGATGAACTCAATGACATACACGAATTCGTTACTGGTGGGCAAGACCTATTTGTAAAGATGGCTACCTCAGAATTAGGTGACATAAAGACAAACAAGAGATCTAGCATGAAGAAGCGGGTGGCTATCATAGTCAGCTTTGGGTTATTAGTCATGGGAGTGCTTATACTAGGATTGGTTTTCTACATACGAAAGAAGAAATTAACAGTATATATGAAGAACATCCATGGAAACACTGAAGGTGGTAATGAAGATATAGAGTTGCTAAAATTTGATTTGGGCACCATATCTAAAGCAACGGATAACTTCTCAGACAATTATAAGCTGGGAGAAGGTGGTTTCGGACCAGTATACAAGGGCACGTTGAAAGAAGGGGAAGACATAGCAGTGAAAAGGCTTTCAAAATGTTCTGGACAAGGAATAAAAGAATTCATGAATGAAGTAATGTTGATTGCTAAACTCCAGCACAGAAATCTTGTAAAGCTTCTTGGTTGCTGCGTTGAAGGAGATGAAAAAATGTTAATTTATGAATACATGCCCAATAGAAGCTTGGACTACTTCATATTTGATGATACAAGGAGTAAATTGCTTGGTTGGGATAAACGCATCAACATTATTGGTGGAATAGCTCGAGGCCTTCTCTATCTTCATCAAGATTCTAGACTGAGGATCATCCATAGAGATCTTAAAACTGGTAATGTTTTACTAGATAAAGATATGAATCCAAAAATATCTGATTTTGGGACGGCTAGAGCATTCGTAGGAGATCAAACATCGGAAAATACTAAGACCGTGGTTGGGACATATGGTTATATGTCTCCTGAGTACGTGGTTGATGGACTCTTCTCAATCAAATCTGATGTCTATAGCTTCGGTGTCATGGTATTGGAGATAGTGAGTGGGAAGAAGAACAGGGGATTCATTCATCCAGACCACCAACTTAACCTTATAGGACATGTATGGACTCTTTGGACTGAAGGAAGATCATTGGAAGTAATGGATAAGAAGTTGGATGGCTCCTATGCTCTAACAGAAGTGTCACGATGCATTCACATAGCTCTGTTGTGTGTGCAACAACAACCAGAAGATAGGCCAAACATGGCATCTGTGGTCCTAATGTTGGGCGGTGAGGGATCATTGCCTGTGCCAAAGCAACCTGGTTTCTTCACTGAAAGGAATCCGTTTGAAGCAGAGAACTCTTCATCAAGCAAATTGGAATCATATTCAATAAATGACATGAGTGTAACAGAGTTAGAGGCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

824

Amino Acids

91.73

Weight (kDa)

7.44

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 82 - 187 3.3e-37 D-mannose binding lectin
S_locus_glycop PF00954 219 - 328 7.5e-28 S-locus glycoprotein domain
PAN_2 PF08276 343 - 410 3.2e-20 PAN-like domain
Pkinase PF00069 508 - 773 1.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 774 7.8e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 781 - 824 8.5e-11 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 906, 1530
AccB1I GGYRCC 1 cut(s) 1490
AccI GTMKAC 3 cut(s) 73, 759, 1560
AccII CGCG 1 cut(s) 867
AciI CCGC 2 cut(s) 1327, 2334
AclWI GGATC 5 cut(s) 445, 566, 1182, 1886, 2350
AcsI RAATTY 5 cut(s) 982, 1131, 1235, 1478, 1637
AcuI CTGAAG 2 cut(s) 1467, 2205
AdeI CACNNNGTG 1 cut(s) 1002
AfaI GTAC 4 cut(s) 58, 127, 221, 2036
AfiI CCNNNNNNNGG 3 cut(s) 581, 2007, 2329
AflIII ACRYGT 1 cut(s) 2164
AhdI GACNNNNNGTC 1 cut(s) 739
AhlI ACTAGT 1 cut(s) 625
AjnI CCWGG 6 cut(s) 184, 499, 580, 998, 1091, 2365
AleI CACNNNNGTG 1 cut(s) 1064
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
Alw21I GWGCWC 3 cut(s) 777, 785, 810
Alw26I GTCTC 2 cut(s) 34, 2031
AlwI GGATC 5 cut(s) 445, 566, 1182, 1886, 2350
Ama87I CYCGRG 2 cut(s) 286, 1839
AoxI GGCC 4 cut(s) 532, 676, 1843, 2300
ApeKI GCWGC 1 cut(s) 1704
ApoI RAATTY 5 cut(s) 982, 1131, 1235, 1478, 1637
ArsI GACNNNNNNTTYG 2 cut(s) 2055, 2087
AseI ATTAAT 1 cut(s) 93
Asp700I GAANNNNTTC 1 cut(s) 9
AspS9I GGNCC 2 cut(s) 1553, 2320
AsuHPI GGTGA 2 cut(s) 1301, 2348
AsuII TTCGAA 1 cut(s) 194
AvaI CYCGRG 2 cut(s) 286, 1839
AvaII GGWCC 2 cut(s) 1553, 2320
BaeGI GKGCMC 2 cut(s) 1493, 1571
BanI GGYRCC 1 cut(s) 1490
BanII GRGCYC 1 cut(s) 777
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BbsI GAAGAC 2 cut(s) 998, 1593
Bbv12I GWGCWC 3 cut(s) 777, 785, 810
BbvI GCAGC 1 cut(s) 1691
BccI CCATC 7 cut(s) 110, 428, 510, 1037, 1264, 2039, 2216
BceAI ACGGC 1 cut(s) 1971
BciT130I CCWGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
BciVI GTATCC 1 cut(s) 627
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 34, 2031
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 7 cut(s) 306, 626, 1314, 1379, 1869, 1916, 1959
BfmI CTRYAG 2 cut(s) 931, 2074
BfuI GTATCC 1 cut(s) 627
BglII AGATCT 3 cut(s) 707, 1310, 1891
BisI GCNGC 1 cut(s) 1705
BlsI GCNGC 1 cut(s) 1706
Bme1390I CCNGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
Bme18I GGWCC 2 cut(s) 1553, 2320
BmeRI GACNNNNNGTC 1 cut(s) 739
BmeT110I CYCGRG 2 cut(s) 286, 1839
BmgT120I GGNCC 2 cut(s) 1553, 2320
BmiI GGNNCC 3 cut(s) 643, 1492, 2227
BmrFI CCNGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
BmrI ACTGGG 1 cut(s) 386
BmsI GCATC 3 cut(s) 1824, 2243, 2321
BmuI ACTGGG 1 cut(s) 386
BpiI GAAGAC 2 cut(s) 998, 1593
BplI GAGNNNNNCTC 2 cut(s) 1833, 1865
BpmI CTGGAG 4 cut(s) 174, 564, 867, 1655
Bpu10I CCTNAGC 1 cut(s) 779
Bpu14I TTCGAA 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 1136
Bsa29I ATCGAT 1 cut(s) 202
BsaAI YACGTR 2 cut(s) 208, 2038
BsaBI GATNNNNATC 1 cut(s) 1929
BsaJI CCNNGG 5 cut(s) 184, 310, 679, 1435, 2001
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 2007, 2329
Bse1I ACTGG 7 cut(s) 69, 392, 535, 718, 1249, 1556, 1906
Bse3DI GCAATG 2 cut(s) 1036, 2345
Bse8I GATNNNNATC 1 cut(s) 1929
BseBI CCWGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
BseCI ATCGAT 1 cut(s) 202
BseDI CCNNGG 5 cut(s) 184, 310, 679, 1435, 2001
BseGI GGATG 6 cut(s) 359, 521, 1431, 1881, 2134, 2227
BseJI GATNNNNATC 1 cut(s) 1929
BseLI CCNNNNNNNGG 3 cut(s) 581, 2007, 2329
BseMI GCAATG 2 cut(s) 1036, 2345
BseMII CTCAG 9 cut(s) 45, 251, 725, 770, 891, 1293, 1533, 1865, 2022
BseNI ACTGG 7 cut(s) 69, 392, 535, 718, 1249, 1556, 1906
BseRI GAGGAG 2 cut(s) 225, 936
BseSI GKGCMC 2 cut(s) 1493, 1571
BseXI GCAGC 1 cut(s) 1691
BseYI CCCAGC 1 cut(s) 1534
Bsh1236I CGCG 1 cut(s) 867
BshFI GGCC 4 cut(s) 534, 678, 1845, 2302
BshNI GGYRCC 1 cut(s) 1490
BshVI ATCGAT 1 cut(s) 202
BsiHKAI GWGCWC 3 cut(s) 777, 785, 810
BsiHKCI CYCGRG 2 cut(s) 286, 1839
BsiSI CCGG 1 cut(s) 1076
BslFI GGGAC 3 cut(s) 533, 1966, 2023
BslI CCNNNNNNNGG 3 cut(s) 581, 2007, 2329
BsmAI GTCTC 2 cut(s) 34, 2031
BsmFI GGGAC 3 cut(s) 533, 1966, 2023
BsmI GAATGC 2 cut(s) 1964, 2256
BsnI GGCC 4 cut(s) 534, 678, 1845, 2302
BsoBI CYCGRG 2 cut(s) 286, 1839
Bsp119I TTCGAA 1 cut(s) 194
Bsp1286I GDGCHC 5 cut(s) 777, 785, 810, 1493, 1571
Bsp19I CCATGG 1 cut(s) 1435
BspACI CCGC 2 cut(s) 1327, 2334
BspANI GGCC 4 cut(s) 534, 678, 1845, 2302
BspCNI CTCAG 9 cut(s) 44, 250, 724, 771, 890, 1292, 1532, 1866, 2023
BspDI ATCGAT 1 cut(s) 202
BspFNI CGCG 1 cut(s) 867
BspHI TCATGA 1 cut(s) 1641
BspLI GGNNCC 3 cut(s) 643, 1492, 2227
BspMAI CTGCAG 1 cut(s) 935
BspPI GGATC 5 cut(s) 445, 566, 1182, 1886, 2350
BspT104I TTCGAA 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 1490
BsrDI GCAATG 2 cut(s) 1036, 2345
BsrI ACTGG 7 cut(s) 69, 392, 535, 718, 1249, 1556, 1906
BssECI CCNNGG 5 cut(s) 184, 310, 679, 1435, 2001
BssNAI GTATAC 3 cut(s) 74, 760, 1561
BssT1I CCWWGG 3 cut(s) 310, 679, 1435
Bst1107I GTATAC 3 cut(s) 74, 760, 1561
Bst2UI CCWGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
Bst4CI ACNGT 3 cut(s) 1124, 1417, 2002
Bst6I CTCTTC 3 cut(s) 47, 2057, 2413
BstBAI YACGTR 2 cut(s) 208, 2038
BstBI TTCGAA 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 961
BstDSI CCRYGG 2 cut(s) 1435, 2001
BstENI CCTNNNNNAGG 1 cut(s) 579
BstF5I GGATG 6 cut(s) 359, 521, 1431, 1881, 2134, 2227
BstFNI CGCG 1 cut(s) 867
BstMAI GTCTC 2 cut(s) 34, 2031
BstNI CCWGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
BstNSI RCATGY 3 cut(s) 963, 1747, 2168
BstSCI CCNGG 6 cut(s) 184, 499, 580, 998, 1091, 2365
BstSFI CTRYAG 2 cut(s) 931, 2074
BstSLI GKGCMC 2 cut(s) 1493, 1571
BstUI CGCG 1 cut(s) 867
BstV1I GCAGC 1 cut(s) 1691
BstV2I GAAGAC 2 cut(s) 998, 1593
BstX2I RGATCY 4 cut(s) 707, 1174, 1310, 1891
BstYI RGATCY 4 cut(s) 707, 1174, 1310, 1891
BstZ17I GTATAC 3 cut(s) 74, 760, 1561
Bsu15I ATCGAT 1 cut(s) 202
BsuI GTATCC 1 cut(s) 627
BsuRI GGCC 4 cut(s) 534, 678, 1845, 2302
BsuTUI ATCGAT 1 cut(s) 202
BtgI CCRYGG 2 cut(s) 1435, 2001
BtsCI GGATG 6 cut(s) 359, 521, 1431, 1881, 2134, 2227
BtsI GCAGTG 3 cut(s) 940, 1155, 1602
BtsIMutI CAGTG 6 cut(s) 899, 940, 1155, 1443, 1602, 2376
Cac8I GCNNGC 1 cut(s) 961
CciI TCATGA 1 cut(s) 1641
Cfr13I GGNCC 2 cut(s) 1553, 2320
ClaI ATCGAT 1 cut(s) 202
CseI GACGC 2 cut(s) 856, 908
CsiI ACCWGGT 2 cut(s) 998, 2365
Csp6I GTAC 4 cut(s) 57, 126, 220, 2035
CviQI GTAC 4 cut(s) 57, 126, 220, 2035
DraIII CACNNNGTG 1 cut(s) 1002
DriI GACNNNNNGTC 1 cut(s) 739
Eam1104I CTCTTC 3 cut(s) 47, 2057, 2413
Eam1105I GACNNNNNGTC 1 cut(s) 739
EarI CTCTTC 3 cut(s) 47, 2057, 2413
Ecl136II GAGCTC 1 cut(s) 775
Eco130I CCWWGG 3 cut(s) 310, 679, 1435
Eco147I AGGCCT 1 cut(s) 1845
Eco24I GRGCYC 1 cut(s) 777
Eco47I GGWCC 2 cut(s) 1553, 2320
Eco53kI GAGCTC 1 cut(s) 775
Eco57I CTGAAG 2 cut(s) 1467, 2205
Eco88I CYCGRG 2 cut(s) 286, 1839
EcoICRI GAGCTC 1 cut(s) 775
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoRI GAATTC 2 cut(s) 1235, 1637
EcoRII CCWGG 6 cut(s) 184, 499, 580, 998, 1091, 2365
EcoT14I CCWWGG 3 cut(s) 310, 679, 1435
EcoT22I ATGCAT 1 cut(s) 2258
EcoT38I GRGCYC 1 cut(s) 777
ErhI CCWWGG 3 cut(s) 310, 679, 1435
FaqI GGGAC 3 cut(s) 533, 1966, 2023
FauI CCCGC 1 cut(s) 1320
FauNDI CATATG 1 cut(s) 2014
FbaI TGATCA 1 cut(s) 280
FblI GTMKAC 3 cut(s) 73, 759, 1560
Fnu4HI GCNGC 1 cut(s) 1705
FokI GGATG 6 cut(s) 346, 528, 1418, 1868, 2121, 2234
FriOI GRGCYC 1 cut(s) 777
Fsp4HI GCNGC 1 cut(s) 1705
FspBI CTAG 7 cut(s) 306, 626, 1314, 1379, 1869, 1916, 1959
GluI GCNGC 1 cut(s) 1705
GsaI CCCAGC 1 cut(s) 1538
GsuI CTGGAG 4 cut(s) 174, 564, 867, 1655
HaeIII GGCC 4 cut(s) 534, 678, 1845, 2302
HapII CCGG 1 cut(s) 1076
HgaI GACGC 2 cut(s) 856, 908
HindIII AAGCTT 2 cut(s) 1691, 1756
HpaII CCGG 1 cut(s) 1076
HphI GGTGA 2 cut(s) 1301, 2348
Hpy166II GTNNAC 6 cut(s) 74, 760, 874, 996, 1059, 1561
Hpy188I TCNGA 9 cut(s) 240, 515, 714, 1282, 1522, 1553, 1945, 1987, 2068
Hpy8I GTNNAC 6 cut(s) 74, 760, 874, 996, 1059, 1561
Hpy99I CGWCG 1 cut(s) 872
HpyAV CCTTC 8 cut(s) 59, 1178, 1442, 1535, 1574, 1706, 1856, 2180
HpyCH4III ACNGT 3 cut(s) 1124, 1417, 2002
HpyCH4IV ACGT 4 cut(s) 207, 992, 1571, 2037
HpySE526I ACGT 4 cut(s) 207, 992, 1571, 2037
Ksp22I TGATCA 1 cut(s) 280
LmnI GCTCC 4 cut(s) 583, 780, 886, 2231
Lsp1109I GCAGC 1 cut(s) 1691
LweI GCATC 3 cut(s) 1824, 2243, 2321
MabI ACCWGGT 2 cut(s) 998, 2365
MaeI CTAG 7 cut(s) 306, 626, 1314, 1379, 1869, 1916, 1959
MaeII ACGT 4 cut(s) 207, 992, 1571, 2037
MaeIII GTNAC 7 cut(s) 386, 485, 896, 1240, 1289, 2247, 2452
MfeI CAATTG 1 cut(s) 1150
MflI RGATCY 4 cut(s) 707, 1174, 1310, 1891
MhlI GDGCHC 5 cut(s) 777, 785, 810, 1493, 1571
MlyI GAGTC 4 cut(s) 419, 443, 2043, 2167
MmeI TCCRAC 2 cut(s) 387, 2199
Mph1103I ATGCAT 1 cut(s) 2258
MroXI GAANNNNTTC 1 cut(s) 9
MseI TTAA 7 cut(s) 93, 524, 1098, 1412, 1730, 1896, 2151
MslI CAYNNNNRTG 3 cut(s) 1064, 1367, 1595
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 1 cut(s) 1076
MspR9I CCNGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
MunI CAATTG 1 cut(s) 1150
Mva1269I GAATGC 2 cut(s) 1964, 2256
MvaI CCWGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
MvnI CGCG 1 cut(s) 867
NcoI CCATGG 1 cut(s) 1435
NdeI CATATG 1 cut(s) 2014
NlaIV GGNNCC 3 cut(s) 643, 1492, 2227
NmuCI GTSAC 4 cut(s) 485, 896, 1289, 2247
NsiI ATGCAT 1 cut(s) 2258
NspI RCATGY 3 cut(s) 963, 1747, 2168
NspV TTCGAA 1 cut(s) 194
OliI CACNNNNGTG 1 cut(s) 1064
PaeI GCATGC 1 cut(s) 963
PaeR7I CTCGAG 1 cut(s) 1839
PagI TCATGA 1 cut(s) 1641
PceI AGGCCT 1 cut(s) 1845
PciI ACATGT 1 cut(s) 2164
PctI GAATGC 2 cut(s) 1964, 2256
PdmI GAANNNNTTC 1 cut(s) 9
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 1 cut(s) 1706
PleI GAGTC 4 cut(s) 418, 443, 2043, 2167
PpsI GAGTC 4 cut(s) 418, 443, 2043, 2167
Ppu21I YACGTR 2 cut(s) 208, 2038
PscI ACATGT 1 cut(s) 2164
PshBI ATTAAT 1 cut(s) 93
PsiI TTATAA 2 cut(s) 906, 1530
Psp124BI GAGCTC 1 cut(s) 777
Psp6I CCWGG 6 cut(s) 184, 499, 580, 998, 1091, 2365
PspFI CCCAGC 1 cut(s) 1534
PspGI CCWGG 6 cut(s) 184, 499, 580, 998, 1091, 2365
PspN4I GGNNCC 3 cut(s) 643, 1492, 2227
PspPI GGNCC 2 cut(s) 1553, 2320
PspXI VCTCGAGB 1 cut(s) 1839
PstI CTGCAG 1 cut(s) 935
PsuI RGATCY 4 cut(s) 707, 1174, 1310, 1891
PvuII CAGCTG 1 cut(s) 152
RsaI GTAC 4 cut(s) 58, 127, 221, 2036
RsaNI GTAC 4 cut(s) 57, 126, 220, 2035
RseI CAYNNNNRTG 3 cut(s) 1064, 1367, 1595
SacI GAGCTC 1 cut(s) 777
SaqAI TTAA 7 cut(s) 93, 524, 1098, 1412, 1730, 1896, 2151
SatI GCNGC 1 cut(s) 1705
Sau96I GGNCC 2 cut(s) 1553, 2320
SchI GAGTC 4 cut(s) 419, 443, 2043, 2167
ScrFI CCNGG 6 cut(s) 186, 501, 582, 1000, 1093, 2367
SduI GDGCHC 5 cut(s) 777, 785, 810, 1493, 1571
SexAI ACCWGGT 2 cut(s) 998, 2365
SfaNI GCATC 3 cut(s) 1824, 2243, 2321
SfcI CTRYAG 2 cut(s) 931, 2074
Sfr274I CTCGAG 1 cut(s) 1839
SfuI TTCGAA 1 cut(s) 194
SinI GGWCC 2 cut(s) 1553, 2320
SlaI CTCGAG 1 cut(s) 1839
SmiMI CAYNNNNRTG 3 cut(s) 1064, 1367, 1595
SmlI CTYRAG 2 cut(s) 1115, 1839
SmoI CTYRAG 2 cut(s) 1115, 1839
SpeI ACTAGT 1 cut(s) 625
SphI GCATGC 1 cut(s) 963
SseBI AGGCCT 1 cut(s) 1845
SsiI CCGC 2 cut(s) 1327, 2334
SspMI CTAG 7 cut(s) 306, 626, 1314, 1379, 1869, 1916, 1959
SstI GAGCTC 1 cut(s) 777
StuI AGGCCT 1 cut(s) 1845
StyD4I CCNGG 6 cut(s) 184, 499, 580, 998, 1091, 2365
StyI CCWWGG 3 cut(s) 310, 679, 1435
TaaI ACNGT 3 cut(s) 1124, 1417, 2002
TaiI ACGT 4 cut(s) 210, 995, 1574, 2040
TaqI TCGA 3 cut(s) 194, 202, 1840
Tru1I TTAA 7 cut(s) 93, 524, 1098, 1412, 1730, 1896, 2151
Tru9I TTAA 7 cut(s) 93, 524, 1098, 1412, 1730, 1896, 2151
TscAI CASTG 6 cut(s) 899, 940, 1162, 1450, 1602, 2383
TseFI GTSAC 4 cut(s) 485, 896, 1289, 2247
TseI GCWGC 1 cut(s) 1704
Tsp45I GTSAC 4 cut(s) 485, 896, 1289, 2247
TspGWI ACGGA 2 cut(s) 1523, 2379
TspRI CASTG 6 cut(s) 899, 940, 1162, 1450, 1602, 2383
VpaK11BI GGWCC 2 cut(s) 1553, 2320
VspI ATTAAT 1 cut(s) 93
XagI CCTNNNNNAGG 1 cut(s) 579
XapI RAATTY 5 cut(s) 982, 1131, 1235, 1478, 1637
XbaI TCTAGA 1 cut(s) 1868
XceI RCATGY 3 cut(s) 963, 1747, 2168
XcmI CCANNNNNNNNNTGG 1 cut(s) 1756
XhoI CTCGAG 1 cut(s) 1839
XmiI GTMKAC 3 cut(s) 73, 759, 1560
XmnI GAANNNNTTC 1 cut(s) 9
XspI CTAG 7 cut(s) 306, 626, 1314, 1379, 1869, 1916, 1959
Zsp2I ATGCAT 1 cut(s) 2258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.