pycom03g16560

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0011543
Physical Location & Seq
Reverse (-)
17710398 .. 17713529
3132 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g16560.1

Sequence Viewer

Length: 2325 bp
ATGGAAATCTTTACAATGCTTTTCGTTTTATCATCTTTCCTCTTCTCTCCAAGCATCTCCGCTACAAAAGATATCGTCACTCCGACAGTATCGCTCAGAGATGGAGAGTCCCTTGTTTCCTCCGGCGGAACATTCGAGCTAGGATTCTTTAGCCCAGGTAATTCGAAGAATCGATACCTTGGAATATGGTTCAATGGAATATCTCCTCAAATTGTTGTGTGGGTGGCCAACAGAGAAACTCCACTTACTGATCACTCTGGAGCTTTGAACATCACTGATGGAGGAGTTCTTGTTCTTTTCAGTGACAAAAATAGCAGTATATGGTCATCTAATTTCACTTCAAGAACCCTAAGGAGCCCGGTTGCTCAGCTCCTGGAATCCGGCAACCTTGTTGTGAAAGACGAAAATGATGACAACTCGGAGAGCACCCTTGTGTGGCAGAGTTTTGATTATCCAGGCAACACCTTACTACCAGGAATGAAGCTTGGATGGAACTCAGCGACAGGTATAAACAGGTCTTTGTCTTCGTGGAAAGCCGCAGAAGATCCATCTCCAGGTGAATTCTCGTATTCCATAGATCATGGCGGATACCCCCAACTAGTCCTACGGAAAGGTCGTGTTCCACACTATAGGTTGGGGTCTTGGAATGGCCTTGGTTTTACTGGGATTCCTGAACTTACTCCTAATGAAGCATTATTTAAAATCGAATTTGTGTCCAATGATAGTGAGGCCTATTACAAATTCGACCTCATGAAAGATACGATGCATTCGAGATTGGTACTCAACCTGTCTGGTGGTTTGCAGCGGTTCTTGTGGATTGATAAAACACATAGCGGGAACATGATTTACTCCGCTCCAGCAGATCGTTGTGACACTTACGATTTGTGTGGTGCATTCGCTTTCTGCAAGCTTGATTCTGATCCTCTATGTTCATGCTTGCAAGGTTTTGTACCAAAATCTCCAAGAGACTACGGGACTTTGAAAAACACGTCTTCAGGATGTCTTCGTAAAGGTCCCTCGGGTTGTACGAATCAAGATGAATTTCAGAAGTTCATACGGGTGAAATTGCCTGACACATCATCGTCACGGATCAATAAAACCATGTCCCTTGAGGAATGTAAGAAAATGTGTTTGACAAACTGCTCTTGCACAGCATATGCAAATTTGGATGTTAGGGGAGGAGGAACTGGCTGTTTGCTTTGGTTCGGCAACCTCACTGATATAAAAGAATTCGACTCTGGTGGGCAAGATCTTTTTGTGCGGATACCCCCCTCCGAATTAGATGGCATTAAGACAAGCAAGCTCTCTGGTGTAAAAAGGAAAGTGGCAATCATAGTCAGCTGTGGGGCAGTAGTCACGGGAGTGCTAATACTAGGATTGGTTTTCTACATACGGAAGAAGAAATTGAAAGCACGAGGAAACATGAAAAACATACGTGAAAAGAACTTTGGTTTCGAAGGTGGAAGCGAGGACATGGAGTTGATAACATTTGATTTGGCCACTGTATCTAGAGCCACTGATAACTTCTCAAACAATAACAAGCTGGGACAAGGTGGTTTCGGACCAGTGTACAAGGTATTAAAAAAGGGGAAGATTCAACTAACATTTATGATTCATTGGTTAGACGATACAAAAAGTAAACTCCTCAATTGGGATCAGCGCATCAACATTATCGGAGGAATTGCTCGAGGACTTCTCTATCTTCATCAGGATTCTAGACTGAGGATCATCCATAGGGATCTTAAAACCAGTAACGTTTTACTAGATAAAGATATGAACCCAAAAATATCTGATTTTGGCACGGCTAGAGCATTCGGGGGAGATCAAACTGAGGAAAATACTAATCGAGTGGTTGGAACATATGGCTACATGTCTCCTGAGTACGTGGTCGATGGACTCTTCTCAGTCAAATCGGATGTTTATAGCTTCGGTGTGATGATGCTGGAGATACTTAGCGGGAAGAAGAATAGGGGATTTCATCATCCAGACCACCAGCTTAACCTTCTAGGACATGCTTGGATGCTTTGGATTGAAGGAAGACCAACAGAAATAATGGATGAGAAGTTAGATGGATCATGTGCGCTGCCAGAAGTGTCACGATGCATTCATATAGCTCTACTGTGTGTGCAGCAACAACCAGAAGATAGGCCAAACATGGCGTCTGTGGTCCTAATGTTGGGTGGGGAGGGTTCATTGCCTATACCCAAGCAACCTGGTTTCTTTACTGAAAGGAACTCGGTTGAAGCAAAGGCTTCTTCGCCAAGCAAGTACGAATCCCATTCCATAAATGAGATGAGTCTAACGCTGTTAGAAGCAAGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

775

Amino Acids

85.69

Weight (kDa)

7.5

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 72 - 178 4.8e-36 D-mannose binding lectin
S_locus_glycop PF00954 210 - 320 2.5e-28 S-locus glycoprotein domain
PAN_2 PF08276 342 - 408 2.2e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 544 - 723 5.5e-26 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 546 - 722 3.3e-27 Protein kinase domain
DUF3403 PF11883 730 - 773 1.2e-10 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 854
AciI CCGC 9 cut(s) 60, 126, 537, 585, 805, 834, 852, 1261, 1956
AclI AACGTT 1 cut(s) 1755
AclWI GGATC 7 cut(s) 539, 914, 1097, 1662, 1733, 1746, 2080
AcoI YGGCCR 2 cut(s) 225, 1497
AcsI RAATTY 6 cut(s) 560, 707, 740, 1040, 1162, 1229
AcuI CTGAAG 1 cut(s) 978
AcyI GRCGYC 1 cut(s) 2159
AfaI GTAC 6 cut(s) 780, 951, 1027, 1571, 1883, 2270
AfiI CCNNNNNNNGG 4 cut(s) 435, 554, 1651, 2176
AflIII ACRYGT 2 cut(s) 987, 1869
AgsI TTSAA 8 cut(s) 193, 268, 342, 982, 1408, 1598, 2033, 2243
AhlI ACTAGT 1 cut(s) 598
AjiI CACGTC 1 cut(s) 990
AjnI CCWGG 6 cut(s) 154, 372, 454, 472, 553, 2212
AjuI GAANNNNNNNTTGG 2 cut(s) 1431, 1463
AleI CACNNNNGTG 2 cut(s) 431, 1361
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
Alw21I GWGCWC 1 cut(s) 428
Alw26I GTCTC 2 cut(s) 960, 1878
AlwI GGATC 7 cut(s) 539, 914, 1097, 1662, 1733, 1746, 2080
AlwNI CAGNNNCTG 1 cut(s) 373
Ama87I CYCGRG 2 cut(s) 1018, 1686
AoxI GGCC 5 cut(s) 225, 649, 729, 1497, 2147
ApeKI GCWGC 3 cut(s) 802, 2083, 2128
ApoI RAATTY 6 cut(s) 560, 707, 740, 1040, 1162, 1229
ArsI GACNNNNNNTTYG 2 cut(s) 2144, 2176
AspLEI GCGC 2 cut(s) 1662, 2083
AspS9I GGNCC 3 cut(s) 1013, 1562, 2167
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 2 cut(s) 569, 1072
AsuII TTCGAA 2 cut(s) 164, 1455
AvaI CYCGRG 2 cut(s) 1018, 1686
AvaII GGWCC 3 cut(s) 1013, 1562, 2167
AxyI CCTNAGG 1 cut(s) 350
BalI TGGCCA 2 cut(s) 227, 1499
BanII GRGCYC 1 cut(s) 359
BarI GAAGNNNNNNTAC 2 cut(s) 158, 190
BauI CACGAG 1 cut(s) 1413
BbsI GAAGAC 4 cut(s) 516, 984, 995, 2044
Bbv12I GWGCWC 1 cut(s) 428
BbvI GCAGC 3 cut(s) 814, 2070, 2140
BccI CCATC 7 cut(s) 95, 272, 483, 556, 1277, 1886, 2063
BceAI ACGGC 1 cut(s) 1818
BciT130I CCWGG 6 cut(s) 156, 374, 456, 474, 555, 2214
BciVI GTATCC 2 cut(s) 581, 1257
BclI TGATCA 1 cut(s) 250
BcnI CCSGG 1 cut(s) 359
BcoDI GTCTC 2 cut(s) 960, 1878
BcuI ACTAGT 1 cut(s) 598
BfaI CTAG 8 cut(s) 140, 599, 1373, 1509, 1716, 1763, 1806, 2006
BfmI CTRYAG 1 cut(s) 628
BfuI GTATCC 2 cut(s) 581, 1257
BglII AGATCT 1 cut(s) 1249
BisI GCNGC 4 cut(s) 537, 803, 2084, 2129
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 4 cut(s) 538, 804, 2085, 2130
Bme1390I CCNGG 7 cut(s) 156, 359, 374, 456, 474, 555, 2214
Bme18I GGWCC 3 cut(s) 1013, 1562, 2167
BmeT110I CYCGRG 2 cut(s) 1018, 1686
BmgBI CACGTC 1 cut(s) 990
BmgT120I GGNCC 3 cut(s) 1013, 1562, 2167
BmiI GGNNCC 2 cut(s) 356, 1015
BmrFI CCNGG 7 cut(s) 156, 359, 374, 456, 474, 555, 2214
BmrI ACTGGG 1 cut(s) 672
BmsI GCATC 6 cut(s) 63, 753, 1671, 1929, 2010, 2090
BmuI ACTGGG 1 cut(s) 672
BpiI GAAGAC 4 cut(s) 516, 984, 995, 2044
BplI GAGNNNNNCTC 2 cut(s) 1680, 1712
BpmI CTGGAG 4 cut(s) 279, 537, 840, 1964
Bpu1102I GCTNAGC 1 cut(s) 366
Bpu14I TTCGAA 2 cut(s) 164, 1455
BpuEI CTTGAG 1 cut(s) 1130
BpuMI CCSGG 1 cut(s) 359
Bsa29I ATCGAT 1 cut(s) 172
BsaAI YACGTR 2 cut(s) 1436, 1885
BsaBI GATNNNNATC 1 cut(s) 918
BsaHI GRCGYC 1 cut(s) 2159
BsaJI CCNNGG 4 cut(s) 154, 178, 652, 1017
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 4 cut(s) 435, 554, 1651, 2176
Bse1I ACTGG 4 cut(s) 667, 1192, 1565, 1749
Bse21I CCTNAGG 1 cut(s) 350
Bse3DI GCAATG 1 cut(s) 2192
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 6 cut(s) 156, 374, 456, 474, 555, 2214
BseCI ATCGAT 1 cut(s) 172
BseDI CCNNGG 4 cut(s) 154, 178, 652, 1017
BseGI GGATG 8 cut(s) 494, 1004, 1174, 1728, 1921, 1981, 2025, 2062
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 4 cut(s) 435, 554, 1651, 2176
BseMI GCAATG 1 cut(s) 2192
BseMII CTCAG 7 cut(s) 109, 380, 510, 1712, 1821, 1869, 1917
BseNI ACTGG 4 cut(s) 667, 1192, 1565, 1749
BseRI GAGGAG 4 cut(s) 195, 297, 1194, 1634
BseXI GCAGC 3 cut(s) 814, 2070, 2140
BseYI CCCAGC 1 cut(s) 1543
BsgI GTGCAG 1 cut(s) 2147
BshFI GGCC 5 cut(s) 227, 651, 731, 1499, 2149
BshVI ATCGAT 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 428
BsiHKCI CYCGRG 2 cut(s) 1018, 1686
BsiSI CCGG 3 cut(s) 123, 359, 381
BslFI GGGAC 5 cut(s) 94, 988, 999, 1090, 1560
BslI CCNNNNNNNGG 4 cut(s) 435, 554, 1651, 2176
BsmAI GTCTC 2 cut(s) 960, 1878
BsmFI GGGAC 5 cut(s) 94, 988, 999, 1090, 1560
BsmI GAATGC 4 cut(s) 766, 893, 1811, 2103
BsnI GGCC 5 cut(s) 227, 651, 731, 1499, 2149
BsoBI CYCGRG 2 cut(s) 1018, 1686
Bsp119I TTCGAA 2 cut(s) 164, 1455
Bsp1286I GDGCHC 2 cut(s) 359, 428
Bsp1407I TGTACA 1 cut(s) 1569
Bsp1720I GCTNAGC 1 cut(s) 366
BspACI CCGC 9 cut(s) 60, 126, 537, 585, 805, 834, 852, 1261, 1956
BspANI GGCC 5 cut(s) 227, 651, 731, 1499, 2149
BspCNI CTCAG 7 cut(s) 108, 379, 509, 1713, 1822, 1870, 1916
BspDI ATCGAT 1 cut(s) 172
BspHI TCATGA 1 cut(s) 750
BspLI GGNNCC 2 cut(s) 356, 1015
BspPI GGATC 7 cut(s) 539, 914, 1097, 1662, 1733, 1746, 2080
BspT104I TTCGAA 2 cut(s) 164, 1455
BsrBI CCGCTC 1 cut(s) 854
BsrDI GCAATG 1 cut(s) 2192
BsrGI TGTACA 1 cut(s) 1569
BsrI ACTGG 4 cut(s) 667, 1192, 1565, 1749
BssECI CCNNGG 4 cut(s) 154, 178, 652, 1017
BssNI GRCGYC 1 cut(s) 2159
BssSI CACGAG 1 cut(s) 1413
BssT1I CCWWGG 2 cut(s) 178, 652
Bst2BI CACGAG 1 cut(s) 1413
Bst2UI CCWGG 6 cut(s) 156, 374, 456, 474, 555, 2214
Bst4CI ACNGT 3 cut(s) 88, 1504, 2121
Bst6I CTCTTC 2 cut(s) 47, 1904
BstACI GRCGYC 1 cut(s) 2159
BstAUI TGTACA 1 cut(s) 1569
BstBAI YACGTR 2 cut(s) 1436, 1885
BstBI TTCGAA 2 cut(s) 164, 1455
BstC8I GCNNGC 3 cut(s) 908, 938, 1301
BstDEI CTNAG 9 cut(s) 95, 350, 366, 496, 1721, 1830, 1878, 1903, 1952
BstF5I GGATG 8 cut(s) 494, 1004, 1174, 1728, 1921, 1981, 2025, 2062
BstHHI GCGC 2 cut(s) 1662, 2083
BstMAI GTCTC 2 cut(s) 960, 1878
BstNI CCWGG 6 cut(s) 156, 374, 456, 474, 555, 2214
BstNSI RCATGY 2 cut(s) 1873, 2015
BstSCI CCNGG 7 cut(s) 154, 357, 372, 454, 472, 553, 2212
BstSFI CTRYAG 1 cut(s) 628
BstV1I GCAGC 3 cut(s) 814, 2070, 2140
BstV2I GAAGAC 4 cut(s) 516, 984, 995, 2044
BstX2I RGATCY 3 cut(s) 544, 1249, 1738
BstYI RGATCY 3 cut(s) 544, 1249, 1738
Bsu15I ATCGAT 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 350
BsuI GTATCC 2 cut(s) 581, 1257
BsuRI GGCC 5 cut(s) 227, 651, 731, 1499, 2149
BsuTUI ATCGAT 1 cut(s) 172
BtrI CACGTC 1 cut(s) 990
BtsCI GGATG 8 cut(s) 494, 1004, 1174, 1728, 1921, 1981, 2025, 2062
BtsIMutI CAGTG 6 cut(s) 273, 307, 1215, 1500, 1515, 1572
Cac8I GCNNGC 3 cut(s) 908, 938, 1301
CaiI CAGNNNCTG 1 cut(s) 373
CciI TCATGA 1 cut(s) 750
CfoI GCGC 2 cut(s) 1662, 2083
Cfr13I GGNCC 3 cut(s) 1013, 1562, 2167
ClaI ATCGAT 1 cut(s) 172
CseI GACGC 1 cut(s) 2148
CsiI ACCWGGT 1 cut(s) 2212
Csp6I GTAC 6 cut(s) 779, 950, 1026, 1570, 1882, 2269
CviQI GTAC 6 cut(s) 779, 950, 1026, 1570, 1882, 2269
DdeI CTNAG 9 cut(s) 95, 350, 366, 496, 1721, 1830, 1878, 1903, 1952
DraI TTTAAA 1 cut(s) 700
EaeI YGGCCR 2 cut(s) 225, 1497
Eam1104I CTCTTC 2 cut(s) 47, 1904
EarI CTCTTC 2 cut(s) 47, 1904
EciI GGCGGA 2 cut(s) 141, 600
Eco130I CCWWGG 2 cut(s) 178, 652
Eco147I AGGCCT 1 cut(s) 731
Eco24I GRGCYC 1 cut(s) 359
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 3 cut(s) 1013, 1562, 2167
Eco57I CTGAAG 1 cut(s) 978
Eco81I CCTNAGG 1 cut(s) 350
Eco88I CYCGRG 2 cut(s) 1018, 1686
EcoO109I RGGNCCY 1 cut(s) 1013
EcoRI GAATTC 2 cut(s) 560, 1229
EcoRII CCWGG 6 cut(s) 154, 372, 454, 472, 553, 2212
EcoRV GATATC 1 cut(s) 73
EcoT14I CCWWGG 2 cut(s) 178, 652
EcoT22I ATGCAT 2 cut(s) 768, 2105
EcoT38I GRGCYC 1 cut(s) 359
ErhI CCWWGG 2 cut(s) 178, 652
FaqI GGGAC 5 cut(s) 94, 988, 999, 1090, 1560
FauI CCCGC 2 cut(s) 827, 1949
FauNDI CATATG 2 cut(s) 1156, 1861
FbaI TGATCA 1 cut(s) 250
Fnu4HI GCNGC 4 cut(s) 537, 803, 2084, 2129
FokI GGATG 8 cut(s) 501, 1011, 1181, 1715, 1928, 1968, 2032, 2069
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 4 cut(s) 537, 803, 2084, 2129
FspBI CTAG 8 cut(s) 140, 599, 1373, 1509, 1716, 1763, 1806, 2006
GlaI GCGC 2 cut(s) 1661, 2082
GluI GCNGC 4 cut(s) 537, 803, 2084, 2129
GsaI CCCAGC 1 cut(s) 1547
GsuI CTGGAG 4 cut(s) 279, 537, 840, 1964
HaeIII GGCC 5 cut(s) 227, 651, 731, 1499, 2149
HapII CCGG 3 cut(s) 123, 359, 381
HgaI GACGC 1 cut(s) 2148
HhaI GCGC 2 cut(s) 1662, 2083
Hin1I GRCGYC 1 cut(s) 2159
Hin6I GCGC 2 cut(s) 1660, 2081
HinP1I GCGC 2 cut(s) 1660, 2081
HindIII AAGCTT 2 cut(s) 482, 908
HpaII CCGG 3 cut(s) 123, 359, 381
HphI GGTGA 2 cut(s) 569, 1072
Hpy166II GTNNAC 2 cut(s) 1570, 1640
Hpy8I GTNNAC 2 cut(s) 1570, 1640
HpyAV CCTTC 3 cut(s) 1451, 2012, 2027
HpyCH4III ACNGT 3 cut(s) 88, 1504, 2121
HpyCH4IV ACGT 4 cut(s) 989, 1435, 1755, 1884
HpyCH4V TGCA 9 cut(s) 766, 802, 893, 906, 940, 1149, 1160, 2103, 2128
HpyF3I CTNAG 9 cut(s) 95, 350, 366, 496, 1721, 1830, 1878, 1903, 1952
HpySE526I ACGT 4 cut(s) 989, 1435, 1755, 1884
Hsp92I GRCGYC 1 cut(s) 2159
HspAI GCGC 2 cut(s) 1660, 2081
Ksp22I TGATCA 1 cut(s) 250
LmnI GCTCC 4 cut(s) 260, 354, 375, 859
Lsp1109I GCAGC 3 cut(s) 814, 2070, 2140
LweI GCATC 6 cut(s) 63, 753, 1671, 1929, 2010, 2090
MabI ACCWGGT 1 cut(s) 2212
MaeI CTAG 8 cut(s) 140, 599, 1373, 1509, 1716, 1763, 1806, 2006
MaeII ACGT 4 cut(s) 989, 1435, 1755, 1884
MaeIII GTNAC 7 cut(s) 76, 302, 869, 1083, 1354, 1751, 2094
MbiI CCGCTC 1 cut(s) 854
MfeI CAATTG 1 cut(s) 1648
MflI RGATCY 3 cut(s) 544, 1249, 1738
MhlI GDGCHC 2 cut(s) 359, 428
MlsI TGGCCA 2 cut(s) 227, 1499
MluNI TGGCCA 2 cut(s) 227, 1499
MlyI GAGTC 4 cut(s) 116, 1229, 1890, 2305
MmeI TCCRAC 2 cut(s) 107, 1834
Mox20I TGGCCA 2 cut(s) 227, 1499
Mph1103I ATGCAT 2 cut(s) 768, 2105
MscI TGGCCA 2 cut(s) 227, 1499
MseI TTAA 6 cut(s) 699, 1290, 1580, 1743, 1998, 2323
MslI CAYNNNNRTG 3 cut(s) 431, 1058, 1361
Msp20I TGGCCA 2 cut(s) 227, 1499
MspA1I CMGCKG 2 cut(s) 805, 1341
MspI CCGG 3 cut(s) 123, 359, 381
MspR9I CCNGG 7 cut(s) 156, 359, 374, 456, 474, 555, 2214
MunI CAATTG 1 cut(s) 1648
Mva1269I GAATGC 4 cut(s) 766, 893, 1811, 2103
MvaI CCWGG 6 cut(s) 156, 374, 456, 474, 555, 2214
NciI CCSGG 1 cut(s) 359
NdeI CATATG 2 cut(s) 1156, 1861
NlaIV GGNNCC 2 cut(s) 356, 1015
NmuCI GTSAC 6 cut(s) 76, 302, 869, 1083, 1354, 2094
NsiI ATGCAT 2 cut(s) 768, 2105
NspI RCATGY 2 cut(s) 1873, 2015
NspV TTCGAA 2 cut(s) 164, 1455
OliI CACNNNNGTG 2 cut(s) 431, 1361
PaeR7I CTCGAG 1 cut(s) 1686
PagI TCATGA 1 cut(s) 750
PceI AGGCCT 1 cut(s) 731
PciI ACATGT 1 cut(s) 1869
PcsI WCGNNNNNNNCGW 3 cut(s) 613, 767, 1025
PctI GAATGC 4 cut(s) 766, 893, 1811, 2103
PfoI TCCNGGA 1 cut(s) 372
PkrI GCNGC 4 cut(s) 538, 804, 2085, 2130
PleI GAGTC 4 cut(s) 115, 1229, 1890, 2304
PpsI GAGTC 4 cut(s) 115, 1229, 1890, 2304
Ppu21I YACGTR 2 cut(s) 1436, 1885
PpuMI RGGWCCY 1 cut(s) 1013
PscI ACATGT 1 cut(s) 1869
Psp1406I AACGTT 1 cut(s) 1755
Psp5II RGGWCCY 1 cut(s) 1013
Psp6I CCWGG 6 cut(s) 154, 372, 454, 472, 553, 2212
PspFI CCCAGC 1 cut(s) 1543
PspGI CCWGG 6 cut(s) 154, 372, 454, 472, 553, 2212
PspN4I GGNNCC 2 cut(s) 356, 1015
PspPI GGNCC 3 cut(s) 1013, 1562, 2167
PspPPI RGGWCCY 1 cut(s) 1013
PspXI VCTCGAGB 1 cut(s) 1686
PsrI GAACNNNNNNTAC 2 cut(s) 830, 862
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 3 cut(s) 544, 1249, 1738
PvuII CAGCTG 1 cut(s) 1341
RsaI GTAC 6 cut(s) 780, 951, 1027, 1571, 1883, 2270
RsaNI GTAC 6 cut(s) 779, 950, 1026, 1570, 1882, 2269
RseI CAYNNNNRTG 3 cut(s) 431, 1058, 1361
SaqAI TTAA 6 cut(s) 699, 1290, 1580, 1743, 1998, 2323
SatI GCNGC 4 cut(s) 537, 803, 2084, 2129
Sau96I GGNCC 3 cut(s) 1013, 1562, 2167
SchI GAGTC 4 cut(s) 116, 1229, 1890, 2305
ScrFI CCNGG 7 cut(s) 156, 359, 374, 456, 474, 555, 2214
SduI GDGCHC 2 cut(s) 359, 428
SexAI ACCWGGT 1 cut(s) 2212
SfaNI GCATC 6 cut(s) 63, 753, 1671, 1929, 2010, 2090
SfcI CTRYAG 1 cut(s) 628
Sfr274I CTCGAG 1 cut(s) 1686
SfuI TTCGAA 2 cut(s) 164, 1455
SinI GGWCC 3 cut(s) 1013, 1562, 2167
SlaI CTCGAG 1 cut(s) 1686
SmiMI CAYNNNNRTG 3 cut(s) 431, 1058, 1361
SmlI CTYRAG 2 cut(s) 1109, 1686
SmoI CTYRAG 2 cut(s) 1109, 1686
SpeI ACTAGT 1 cut(s) 598
SseBI AGGCCT 1 cut(s) 731
SsiI CCGC 9 cut(s) 60, 126, 537, 585, 805, 834, 852, 1261, 1956
SspMI CTAG 8 cut(s) 140, 599, 1373, 1509, 1716, 1763, 1806, 2006
StuI AGGCCT 1 cut(s) 731
StyD4I CCNGG 7 cut(s) 154, 357, 372, 454, 472, 553, 2212
StyI CCWWGG 2 cut(s) 178, 652
TaaI ACNGT 3 cut(s) 88, 1504, 2121
TaiI ACGT 4 cut(s) 992, 1438, 1758, 1887
TatI WGTACW 1 cut(s) 1569
TauI GCSGC 1 cut(s) 539
Tru1I TTAA 6 cut(s) 699, 1290, 1580, 1743, 1998, 2323
Tru9I TTAA 6 cut(s) 699, 1290, 1580, 1743, 1998, 2323
TscAI CASTG 6 cut(s) 280, 307, 1222, 1507, 1522, 1572
TseFI GTSAC 6 cut(s) 76, 302, 869, 1083, 1354, 2094
TseI GCWGC 3 cut(s) 802, 2083, 2128
Tsp45I GTSAC 6 cut(s) 76, 302, 869, 1083, 1354, 2094
TspGWI ACGGA 3 cut(s) 622, 1102, 1408
TspRI CASTG 6 cut(s) 280, 307, 1222, 1507, 1522, 1572
VpaK11BI GGWCC 3 cut(s) 1013, 1562, 2167
XapI RAATTY 6 cut(s) 560, 707, 740, 1040, 1162, 1229
XbaI TCTAGA 2 cut(s) 1508, 1715
XceI RCATGY 2 cut(s) 1873, 2015
XhoI CTCGAG 1 cut(s) 1686
XspI CTAG 8 cut(s) 140, 599, 1373, 1509, 1716, 1763, 1806, 2006
Zsp2I ATGCAT 2 cut(s) 768, 2105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.