Rw5G022880

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011543
Physical Location & Seq
Forward (+)
31884627 .. 31888118
3492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022880.1

Sequence Viewer

Length: 2463 bp
ATGGAAATCTTCCCAATGCATTTTGTTTTCTCAGCAATTATCTTCTCCCTTCTAAGTACCTACACTACCAGTATACAAGACATCATTACCATTAATCCAAGAGTATCAATCAAAGATGGAGGGAGTACCCTAGTTTCAGCTGGAGGAAGCTTCGAATTGGGATTCTTTAGCCCAGGTAATTCGAAGAGTCGATACGTGGGAATCTGGTACAATAACATATCTCCTCAGAAACTTGTATGGGTGTGCAACAAAGAAACTCCCCTTACTGATCACTCGGGAGCTTTGAATCTCACTAGCCAAGGAGTTCTCGTTCTGTTCAATGGCAAAAATAGTATTATCTGGTCATCATCCAACGAGACTTCAAGCAACATAAGTAACCCAGTTGCTCAACTCTTGGAGTCGGGCAATTTCGTGGTGAAAGATGGATCAGAGGAGGACTCAAAAGTTCTGTGGCAGAGCTTTGACTATCCAAGTGACACCTTACTACCAGGAATGAAGATCGGATGGGACTTAAAAACCGGCCTAAACAGGTTTCTGTCATCTTGGAAAGAACCAGAAGATCCTGCTCCAGGACAATTTTCATTCTCTTTAGATCGTAGTGGGTATCCCCAACTAGTTGTTAGGAATGGTTCCATAGCACATTACAGGTTGGGGTCATGGAATGGCCTTGGCTTCACAGGAACTCCTCAACTTGGATCTCTGAACCAGTTGTTCAGACTTGACTTTGTGTCAAATGAGAATGAAGTATACTACAAATATGAGCTCCGGAGCACTTCAGTTGAATCAAGATTAGTGCTCAACAGATCAGGCGGTTTGCAACGCTTCATGTGGAGTGGCACAACAAATATCAATCGCGTCATTTACTCAGCTCCGGTAGATAAGTGTGACGCTTATAATGTGTGCGGTGCATTTTCTCTCTGCAGTGTTGATTCTCCTCGATTATGTGCATGCTTACAAGGTTTCGGACCAAATTCTTCAACGTCCACCAGGTGTCTTCGTAGAACTCCATTGAGTTGCAATGATTACCAAGATGGCTTCAAGAAGTTCACAAGGGTGAAATTGCCGGACACATCTTCGTCCTGGTTTGACTTGACAATGACCCTTGAGGACTGTGAGAAAACTTGTTTGAAGAACTGCACTTGCACTGCTTATGCAAATTTGGATGTAAGGGAAGGAGGAAGTGGCTGTTTGCTCTGGTTTGATGAACTCAATGACATACACGAATTTGATACTGGTGGGCAAGACCTCTTTGTAAAGATGGCTACCTCAGAATTAGGTGACATAAAGACAAACAAGAGATCTAGCATGAAGAAGCGGCTGGCTATCATAGTCAGCTTTGGGTTATTAGTCATGGGAGTGCTTATACTAGGATTGGTTTTCTACATACGAAAGAAGAAATTAACAGTATATATGAAGAACATCCATGGAAACACTGAAGGTGGTAATGAAGATATAGAGTTGCTAAAATTTGATTTGGGCACCATATCTAAAGCAACGGATAACTTCTCAGACAATTATAAGCTAGGAGAAGGTGGTTTCGGACCAGTATACAAGGGCACGTTGAAAGAAGGGGAAGACATAGCAGTGAAAAGGCTTTCAAAATGTTCTGGACAAGGAATAAAAGAATTCATGAATGAAGTAATGTTGATTGCTAAACTCCAGCACAGAAATCTTGTAAAGCTTCTTGGTTGCTGCGTTGAAGGAGATGAAAAAATGTTAATTTATGAATACATGCCCAATAGAAGCTTGGACTACTTCATATTTGGTGATACAAGAAGTAAATTGCTTGGTTGGGATCAACGCATCAACATTATTGGTGGAATAGCTCGAGGCCTTCTCTATCTTCATCAAGATTCTAGACTGAGGATCATCCATAGAGATCTTAAAACTGGTAATGTTTTACTAGATAAAGACATGAACCCAAAAATATCTGATTTTGGGACGGCTAGAGCATTCGTAGGAGATCAAACTGCGGAAAATACTAAGACCGTGGTTGGGACATATGGTTATATGTCTCCTGAGTACATGGTTGATGGACTCTTCTCAATCAAATCTGATGTCTATAGCTTCGGTGTCATGGTATTGGAGATAGTGAGTGGGAAGAAGAACAGGGGATTCATTCATCCGGACCACCAACTTAACCTTATAGGATATGTATGGACTCTATGGACTGAAGGAAGATCATTGGAAGTAATGGATAAGAAGTTAGATGGCTCCTATGCTCTAACAGAAGTGTCACGATGCATTCACATAGCTCTGTTGTGTGTGCAACAACAACCAGAAGATAGACCAAACATGGCATCTGTGGTCTTAATGTTGGGCGGTGAGGGATCATTGCCTGTGCCAAAGCAACCTGGTTTCTTCACTGAAAGGAATCCGTTTGAAGCAGAGAACTCTTCATCAAGCAAATTGGAATCATATTCAATAAATGACATGAGTATAACAGAGTTAGAGGCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

820

Amino Acids

91.43

Weight (kDa)

6.21

Isoelectric Point (pI)

43.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 78 - 183 5.9e-37 D-mannose binding lectin
S_locus_glycop PF00954 215 - 324 2.6e-26 S-locus glycoprotein domain
PAN_2 PF08276 339 - 406 1.9e-21 PAN-like domain
Pkinase PF00069 504 - 769 5.4e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 506 - 770 9.4e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 777 - 820 8e-11 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 894, 1518
AccB1I GGYRCC 1 cut(s) 1478
AccI GTMKAC 3 cut(s) 73, 747, 1548
AccII CGCG 1 cut(s) 855
AccIII TCCGGA 2 cut(s) 765, 2125
AciI CCGC 5 cut(s) 810, 903, 1315, 1973, 2322
AclWI GGATC 6 cut(s) 433, 554, 703, 1803, 1874, 2338
AcsI RAATTY 5 cut(s) 970, 1156, 1223, 1466, 1625
AcuI CTGAAG 3 cut(s) 759, 1455, 2193
AdeI CACNNNGTG 1 cut(s) 990
AfaI GTAC 4 cut(s) 58, 127, 209, 2024
AfiI CCNNNNNNNGG 3 cut(s) 569, 692, 1995
AhdI GACNNNNNGTC 1 cut(s) 727
AhlI ACTAGT 1 cut(s) 613
AjnI CCWGG 6 cut(s) 172, 487, 568, 986, 1079, 2353
AleI CACNNNNGTG 1 cut(s) 1052
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
Alw21I GWGCWC 3 cut(s) 765, 773, 798
Alw26I GTCTC 2 cut(s) 350, 2019
AlwI GGATC 6 cut(s) 433, 554, 703, 1803, 1874, 2338
Ama87I CYCGRG 2 cut(s) 274, 1827
Aor13HI TCCGGA 2 cut(s) 765, 2125
AoxI GGCC 3 cut(s) 520, 664, 1831
ApeKI GCWGC 1 cut(s) 1692
ApoI RAATTY 5 cut(s) 970, 1156, 1223, 1466, 1625
ArsI GACNNNNNNTTYG 2 cut(s) 2043, 2075
AseI ATTAAT 1 cut(s) 93
AspS9I GGNCC 3 cut(s) 965, 1541, 2128
AsuHPI GGTGA 5 cut(s) 427, 1066, 1289, 1778, 2336
AsuII TTCGAA 2 cut(s) 153, 182
AvaI CYCGRG 2 cut(s) 274, 1827
AvaII GGWCC 3 cut(s) 965, 1541, 2128
BaeGI GKGCMC 2 cut(s) 1481, 1559
BanI GGYRCC 1 cut(s) 1478
BanII GRGCYC 1 cut(s) 765
BarI GAAGNNNNNNTAC 2 cut(s) 176, 208
BbsI GAAGAC 2 cut(s) 986, 1581
Bbv12I GWGCWC 3 cut(s) 765, 773, 798
BbvI GCAGC 1 cut(s) 1679
BccI CCATC 7 cut(s) 110, 416, 498, 1025, 1252, 2027, 2204
BceAI ACGGC 1 cut(s) 1959
BciT130I CCWGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
BciVI GTATCC 1 cut(s) 615
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 350, 2019
BcuI ACTAGT 1 cut(s) 613
BfaI CTAG 9 cut(s) 131, 294, 614, 1302, 1367, 1523, 1857, 1904, 1947
BfmI CTRYAG 2 cut(s) 919, 2062
BfuI GTATCC 1 cut(s) 615
BglII AGATCT 2 cut(s) 1298, 1879
BisI GCNGC 2 cut(s) 1316, 1693
BlsI GCNGC 2 cut(s) 1317, 1694
Bme1390I CCNGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
Bme18I GGWCC 3 cut(s) 965, 1541, 2128
BmeRI GACNNNNNGTC 1 cut(s) 727
BmeT110I CYCGRG 2 cut(s) 274, 1827
BmgT120I GGNCC 3 cut(s) 965, 1541, 2128
BmiI GGNNCC 3 cut(s) 631, 1480, 2215
BmrFI CCNGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
BmrI ACTGGG 1 cut(s) 374
BmsI GCATC 3 cut(s) 1812, 2231, 2309
BmuI ACTGGG 1 cut(s) 374
BpiI GAAGAC 2 cut(s) 986, 1581
BplI GAGNNNNNCTC 4 cut(s) 422, 454, 1821, 1853
BpmI CTGGAG 3 cut(s) 162, 552, 1643
Bpu14I TTCGAA 2 cut(s) 153, 182
BpuEI CTTGAG 1 cut(s) 1124
BsaAI YACGTR 1 cut(s) 196
BsaJI CCNNGG 5 cut(s) 172, 298, 667, 1423, 1989
BsaWI WCCGGW 3 cut(s) 765, 871, 2125
BsaXI ACNNNNNCTCC 4 cut(s) 294, 324, 667, 697
Bsc4I CCNNNNNNNGG 3 cut(s) 569, 692, 1995
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 6 cut(s) 69, 380, 706, 1237, 1544, 1894
Bse3DI GCAATG 2 cut(s) 1024, 2333
BseAI TCCGGA 2 cut(s) 765, 2125
BseBI CCWGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
BseDI CCNNGG 5 cut(s) 172, 298, 667, 1423, 1989
BseGI GGATG 6 cut(s) 347, 509, 1168, 1419, 1869, 2122
BseLI CCNNNNNNNGG 3 cut(s) 569, 692, 1995
BseMI GCAATG 2 cut(s) 1024, 2333
BseMII CTCAG 7 cut(s) 45, 239, 879, 1281, 1521, 1853, 2010
BseNI ACTGG 6 cut(s) 69, 380, 706, 1237, 1544, 1894
BseRI GAGGAG 4 cut(s) 213, 446, 675, 924
BseSI GKGCMC 2 cut(s) 1481, 1559
BseXI GCAGC 1 cut(s) 1679
BsgI GTGCAG 1 cut(s) 1120
Bsh1236I CGCG 1 cut(s) 855
BshFI GGCC 3 cut(s) 522, 666, 1833
BshNI GGYRCC 1 cut(s) 1478
BsiHKAI GWGCWC 3 cut(s) 765, 773, 798
BsiHKCI CYCGRG 2 cut(s) 274, 1827
BsiSI CCGG 5 cut(s) 519, 766, 872, 1064, 2126
BslFI GGGAC 3 cut(s) 521, 1954, 2011
BslI CCNNNNNNNGG 3 cut(s) 569, 692, 1995
BsmAI GTCTC 2 cut(s) 350, 2019
BsmFI GGGAC 3 cut(s) 521, 1954, 2011
BsmI GAATGC 2 cut(s) 1952, 2244
BsnI GGCC 3 cut(s) 522, 666, 1833
BsoBI CYCGRG 2 cut(s) 274, 1827
Bsp119I TTCGAA 2 cut(s) 153, 182
Bsp1286I GDGCHC 5 cut(s) 765, 773, 798, 1481, 1559
Bsp13I TCCGGA 2 cut(s) 765, 2125
Bsp19I CCATGG 1 cut(s) 1423
BspACI CCGC 5 cut(s) 810, 903, 1315, 1973, 2322
BspANI GGCC 3 cut(s) 522, 666, 1833
BspCNI CTCAG 7 cut(s) 44, 238, 878, 1280, 1520, 1854, 2011
BspEI TCCGGA 2 cut(s) 765, 2125
BspFNI CGCG 1 cut(s) 855
BspHI TCATGA 1 cut(s) 1629
BspLI GGNNCC 3 cut(s) 631, 1480, 2215
BspMAI CTGCAG 1 cut(s) 923
BspPI GGATC 6 cut(s) 433, 554, 703, 1803, 1874, 2338
BspT104I TTCGAA 2 cut(s) 153, 182
BspT107I GGYRCC 1 cut(s) 1478
BsrDI GCAATG 2 cut(s) 1024, 2333
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 6 cut(s) 69, 380, 706, 1237, 1544, 1894
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 5 cut(s) 172, 298, 667, 1423, 1989
BssNAI GTATAC 3 cut(s) 74, 748, 1549
BssT1I CCWWGG 3 cut(s) 298, 667, 1423
Bst1107I GTATAC 3 cut(s) 74, 748, 1549
Bst2UI CCWGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
Bst4CI ACNGT 3 cut(s) 1112, 1405, 1990
Bst6I CTCTTC 3 cut(s) 179, 2045, 2401
BstBAI YACGTR 1 cut(s) 196
BstBI TTCGAA 2 cut(s) 153, 182
BstC8I GCNNGC 2 cut(s) 949, 1320
BstDEI CTNAG 9 cut(s) 31, 53, 225, 865, 1267, 1507, 1862, 1983, 2019
BstDSI CCRYGG 2 cut(s) 1423, 1989
BstENI CCTNNNNNAGG 1 cut(s) 567
BstF5I GGATG 6 cut(s) 347, 509, 1168, 1419, 1869, 2122
BstFNI CGCG 1 cut(s) 855
BstMAI GTCTC 2 cut(s) 350, 2019
BstNI CCWGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
BstNSI RCATGY 2 cut(s) 951, 1735
BstSCI CCNGG 6 cut(s) 172, 487, 568, 986, 1079, 2353
BstSFI CTRYAG 2 cut(s) 919, 2062
BstSLI GKGCMC 2 cut(s) 1481, 1559
BstUI CGCG 1 cut(s) 855
BstV1I GCAGC 1 cut(s) 1679
BstV2I GAAGAC 2 cut(s) 986, 1581
BstX2I RGATCY 4 cut(s) 559, 695, 1298, 1879
BstYI RGATCY 4 cut(s) 559, 695, 1298, 1879
BstZ17I GTATAC 3 cut(s) 74, 748, 1549
BsuI GTATCC 1 cut(s) 615
BsuRI GGCC 3 cut(s) 522, 666, 1833
BtgI CCRYGG 2 cut(s) 1423, 1989
BtsCI GGATG 6 cut(s) 347, 509, 1168, 1419, 1869, 2122
BtsI GCAGTG 3 cut(s) 928, 1143, 1590
BtsIMutI CAGTG 5 cut(s) 928, 1143, 1431, 1590, 2364
Cac8I GCNNGC 2 cut(s) 949, 1320
CciI TCATGA 1 cut(s) 1629
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 3 cut(s) 965, 1541, 2128
CseI GACGC 2 cut(s) 844, 896
CsiI ACCWGGT 2 cut(s) 986, 2353
Csp6I GTAC 4 cut(s) 57, 126, 208, 2023
CviQI GTAC 4 cut(s) 57, 126, 208, 2023
DdeI CTNAG 9 cut(s) 31, 53, 225, 865, 1267, 1507, 1862, 1983, 2019
DraIII CACNNNGTG 1 cut(s) 990
DriI GACNNNNNGTC 1 cut(s) 727
Eam1104I CTCTTC 3 cut(s) 179, 2045, 2401
Eam1105I GACNNNNNGTC 1 cut(s) 727
EarI CTCTTC 3 cut(s) 179, 2045, 2401
Ecl136II GAGCTC 1 cut(s) 763
Eco130I CCWWGG 3 cut(s) 298, 667, 1423
Eco147I AGGCCT 1 cut(s) 1833
Eco24I GRGCYC 1 cut(s) 765
Eco47I GGWCC 3 cut(s) 965, 1541, 2128
Eco53kI GAGCTC 1 cut(s) 763
Eco57I CTGAAG 3 cut(s) 759, 1455, 2193
Eco88I CYCGRG 2 cut(s) 274, 1827
EcoICRI GAGCTC 1 cut(s) 763
EcoNI CCTNNNNNAGG 1 cut(s) 567
EcoRI GAATTC 1 cut(s) 1625
EcoRII CCWGG 6 cut(s) 172, 487, 568, 986, 1079, 2353
EcoT14I CCWWGG 3 cut(s) 298, 667, 1423
EcoT22I ATGCAT 2 cut(s) 21, 2246
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 3 cut(s) 298, 667, 1423
FaqI GGGAC 3 cut(s) 521, 1954, 2011
FauNDI CATATG 1 cut(s) 2002
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 3 cut(s) 73, 747, 1548
Fnu4HI GCNGC 2 cut(s) 1316, 1693
FokI GGATG 6 cut(s) 334, 516, 1175, 1406, 1856, 2109
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 2 cut(s) 1316, 1693
FspBI CTAG 9 cut(s) 131, 294, 614, 1302, 1367, 1523, 1857, 1904, 1947
GluI GCNGC 2 cut(s) 1316, 1693
GsuI CTGGAG 3 cut(s) 162, 552, 1643
HaeIII GGCC 3 cut(s) 522, 666, 1833
HapII CCGG 5 cut(s) 519, 766, 872, 1064, 2126
HgaI GACGC 2 cut(s) 844, 896
HindIII AAGCTT 3 cut(s) 148, 1679, 1744
HpaII CCGG 5 cut(s) 519, 766, 872, 1064, 2126
HphI GGTGA 5 cut(s) 427, 1066, 1289, 1778, 2336
Hpy166II GTNNAC 5 cut(s) 74, 748, 984, 1047, 1549
Hpy8I GTNNAC 5 cut(s) 74, 748, 984, 1047, 1549
HpyAV CCTTC 8 cut(s) 59, 1166, 1430, 1523, 1562, 1694, 1844, 2168
HpyCH4III ACNGT 3 cut(s) 1112, 1405, 1990
HpyCH4IV ACGT 3 cut(s) 195, 980, 1559
HpyF3I CTNAG 9 cut(s) 31, 53, 225, 865, 1267, 1507, 1862, 1983, 2019
HpySE526I ACGT 3 cut(s) 195, 980, 1559
Kpn2I TCCGGA 2 cut(s) 765, 2125
Ksp22I TGATCA 1 cut(s) 268
LmnI GCTCC 6 cut(s) 278, 571, 768, 768, 874, 2219
Lsp1109I GCAGC 1 cut(s) 1679
LweI GCATC 3 cut(s) 1812, 2231, 2309
MabI ACCWGGT 2 cut(s) 986, 2353
MaeI CTAG 9 cut(s) 131, 294, 614, 1302, 1367, 1523, 1857, 1904, 1947
MaeII ACGT 3 cut(s) 195, 980, 1559
MaeIII GTNAC 5 cut(s) 374, 473, 884, 1277, 2235
MflI RGATCY 4 cut(s) 559, 695, 1298, 1879
MhlI GDGCHC 5 cut(s) 765, 773, 798, 1481, 1559
MlyI GAGTC 5 cut(s) 196, 407, 431, 2031, 2155
MmeI TCCRAC 1 cut(s) 375
Mph1103I ATGCAT 2 cut(s) 21, 2246
MroI TCCGGA 2 cut(s) 765, 2125
MseI TTAA 7 cut(s) 93, 512, 1400, 1718, 1884, 2139, 2312
MslI CAYNNNNRTG 3 cut(s) 1052, 1355, 1583
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 5 cut(s) 519, 766, 872, 1064, 2126
MspR9I CCNGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
Mva1269I GAATGC 2 cut(s) 1952, 2244
MvaI CCWGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
MvnI CGCG 1 cut(s) 855
NcoI CCATGG 1 cut(s) 1423
NdeI CATATG 1 cut(s) 2002
NlaIV GGNNCC 3 cut(s) 631, 1480, 2215
NmuCI GTSAC 4 cut(s) 473, 884, 1277, 2235
NsiI ATGCAT 2 cut(s) 21, 2246
NspI RCATGY 2 cut(s) 951, 1735
NspV TTCGAA 2 cut(s) 153, 182
OliI CACNNNNGTG 1 cut(s) 1052
PaeI GCATGC 1 cut(s) 951
PaeR7I CTCGAG 1 cut(s) 1827
PagI TCATGA 1 cut(s) 1629
PceI AGGCCT 1 cut(s) 1833
PctI GAATGC 2 cut(s) 1952, 2244
PfeI GAWTC 9 cut(s) 162, 201, 286, 782, 929, 1853, 2115, 2374, 2414
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 2 cut(s) 1317, 1694
PleI GAGTC 5 cut(s) 195, 406, 431, 2031, 2155
PpsI GAGTC 5 cut(s) 195, 406, 431, 2031, 2155
Ppu21I YACGTR 1 cut(s) 196
PshBI ATTAAT 1 cut(s) 93
PsiI TTATAA 2 cut(s) 894, 1518
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 6 cut(s) 172, 487, 568, 986, 1079, 2353
PspGI CCWGG 6 cut(s) 172, 487, 568, 986, 1079, 2353
PspN4I GGNNCC 3 cut(s) 631, 1480, 2215
PspPI GGNCC 3 cut(s) 965, 1541, 2128
PspXI VCTCGAGB 1 cut(s) 1827
PstI CTGCAG 1 cut(s) 923
PsuI RGATCY 4 cut(s) 559, 695, 1298, 1879
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 4 cut(s) 58, 127, 209, 2024
RsaNI GTAC 4 cut(s) 57, 126, 208, 2023
RseI CAYNNNNRTG 3 cut(s) 1052, 1355, 1583
SacI GAGCTC 1 cut(s) 765
SaqAI TTAA 7 cut(s) 93, 512, 1400, 1718, 1884, 2139, 2312
SatI GCNGC 2 cut(s) 1316, 1693
Sau96I GGNCC 3 cut(s) 965, 1541, 2128
SchI GAGTC 5 cut(s) 196, 407, 431, 2031, 2155
ScrFI CCNGG 6 cut(s) 174, 489, 570, 988, 1081, 2355
SduI GDGCHC 5 cut(s) 765, 773, 798, 1481, 1559
SexAI ACCWGGT 2 cut(s) 986, 2353
SfaNI GCATC 3 cut(s) 1812, 2231, 2309
SfcI CTRYAG 2 cut(s) 919, 2062
Sfr274I CTCGAG 1 cut(s) 1827
SfuI TTCGAA 2 cut(s) 153, 182
SinI GGWCC 3 cut(s) 965, 1541, 2128
SlaI CTCGAG 1 cut(s) 1827
SmiMI CAYNNNNRTG 3 cut(s) 1052, 1355, 1583
SmlI CTYRAG 2 cut(s) 1103, 1827
SmoI CTYRAG 2 cut(s) 1103, 1827
SpeI ACTAGT 1 cut(s) 613
SphI GCATGC 1 cut(s) 951
SseBI AGGCCT 1 cut(s) 1833
SsiI CCGC 5 cut(s) 810, 903, 1315, 1973, 2322
SspMI CTAG 9 cut(s) 131, 294, 614, 1302, 1367, 1523, 1857, 1904, 1947
SstI GAGCTC 1 cut(s) 765
StuI AGGCCT 1 cut(s) 1833
StyD4I CCNGG 6 cut(s) 172, 487, 568, 986, 1079, 2353
StyI CCWWGG 3 cut(s) 298, 667, 1423
TaaI ACNGT 3 cut(s) 1112, 1405, 1990
TaiI ACGT 3 cut(s) 198, 983, 1562
TaqI TCGA 5 cut(s) 153, 182, 190, 937, 1828
TatI WGTACW 1 cut(s) 2022
TauI GCSGC 1 cut(s) 1318
TfiI GAWTC 9 cut(s) 162, 201, 286, 782, 929, 1853, 2115, 2374, 2414
Tru1I TTAA 7 cut(s) 93, 512, 1400, 1718, 1884, 2139, 2312
Tru9I TTAA 7 cut(s) 93, 512, 1400, 1718, 1884, 2139, 2312
TscAI CASTG 5 cut(s) 928, 1150, 1438, 1590, 2371
TseFI GTSAC 4 cut(s) 473, 884, 1277, 2235
TseI GCWGC 1 cut(s) 1692
Tsp45I GTSAC 4 cut(s) 473, 884, 1277, 2235
TspGWI ACGGA 2 cut(s) 1511, 2367
TspRI CASTG 5 cut(s) 928, 1150, 1438, 1590, 2371
VpaK11BI GGWCC 3 cut(s) 965, 1541, 2128
VspI ATTAAT 1 cut(s) 93
XagI CCTNNNNNAGG 1 cut(s) 567
XapI RAATTY 5 cut(s) 970, 1156, 1223, 1466, 1625
XbaI TCTAGA 1 cut(s) 1856
XceI RCATGY 2 cut(s) 951, 1735
XcmI CCANNNNNNNNNTGG 1 cut(s) 1744
XhoI CTCGAG 1 cut(s) 1827
XmiI GTMKAC 3 cut(s) 73, 747, 1548
XspI CTAG 9 cut(s) 131, 294, 614, 1302, 1367, 1523, 1857, 1904, 1947
Zsp2I ATGCAT 2 cut(s) 21, 2246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.