Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0012976

Physical Location & Seq

Genomic Coordinates Reverse (-)

26969063 .. 26976059

Gene Structure 6997 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1197500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2979 bp

ATGCAAATGCTTATTCCACAAGTCACAAACCTCCGTCTCCTCTTCTTTTCCCCTTTCCCTTGTCTCTCCTCCCTCTCTCCTCTCACCATTAAACCCTATAAACCCCACTCTCTCTTCACCGCCCTCGCTTCCTCCTACCGAAAACGACACCGTTCCCTCAACCAACCTCCCAACCTCCGCGCCAGGAACAAAAGCACTCTCAGAGAGAGCGGAGGAGGCAAAGACAAATCAATGGAGGAGGCCACGGAGGCTAAGAAGGCTGACACTGCTGGGTTCAATAAGCGAAGAGCTGAGGGCAACGACAAGAGTGATAGGCCGAAGAAGAACCTTCAGCGGAAAGTGCGCTCCCTTAATCCCATCAATACCCTATCTTATGTTCAGATACTAGGGACTGGAATGGATACTCATGATACATCTCCTTCAGTGTTGCTATTCTTTGACAAACAGAGATTTATATTTAATGCTGGAGAGGGTTTGCAAAGGTTCTGCACAGAGCATAAGATTAAGTTGTCAAAGATTGATCACATTTTTCTCTCTCGTGTCTGCTCAGAGACTGCAGGTGGACTTCCAGGTTTGCTGTTAACTTTGGCTGGCATGGGTGAAGAAGGAATGTCTGTCAATGTATGGGGTCCGTCAGATCTCAAATATCTGGTAGATGCCATGAGGTGTTTCATCCCAAATGCTGCCATGGTTCACACTCGGAGCTTTGGGCCGACTCTCGGTTCTGTGGGGGGTTTTATGGATGATCAAACTAAGTTTAAAGAGCCGATTGTTCTTGTTAATGATGAGGTTGTCAATATATCAGCCATTCTTCTACTCCCAAATTTCTCAAAAGGGACTGGGCTCCCAAATGAAGTAACCAGTACACAGAATCCGACAGAAAAGGTTCTTGATGATAGGATTGGTCATGCTTCACAACCCTTTAGACTAAATGGCAACAGTAGACCTAATGCAAAGCCTGGTGACATGTCTGTCGTTTACATCTGTGAATTACCTGAGATTAAAGGGAAATTTGACCCTGAAAAAGCTAAGGCTTTGGGTCTTAAACCAGGGTCAAAATATCGGGAACTGCAACTTGGAAATTCAGTGCACTCAGATTTTCAAAATATCACGGTTCATCCAAGTGATGTAATGGAACCCTCCATTCCTGGTCCAATTGTATTACTTGTAGACTGCCCAACGGAATCTCATTTGGAGGAACTGGTATCCATAAAATGTCTCAGTCGCTATTATGTGGATTTCTCAGGCCCGCCTGAGAATGCTAAGGGCGTGACTTGTTTCATTCATTTGGGTCCTGCATCTCTAATAAGCAGTCCGAACTACCAGAGCTGGATGAAGAGATTTGGTTTAGCCCAACATATCATGGCTGGGAATGAAAGGAAGAATGTGGAGATTCCAATCCTAAGAGCAAGTGCAAGAATTGCAGCTCGACTTAACTATCTATGTCCACAGTTCTTTCCAGCTCCAGGTTTTTGGTCTCTTCAGAACCTTGATTGTTTGGCACCAGAATCCACTGCTTCAAGTGAGGGTTCCATTTCAAAGTCTTGTGAAGGCATTTCCGCTGAAAACCTCCTCAAGTTCACGTTGCGTCCATATGCTCGCCTTGGATTAGATAGGTCTGTTATTCCAAGTCAAGTGGCTTCCTCAGAAATCATTGATGAGTTAATTTCAGAGATTCCGGAGGTTGTAGATGCAGCCCATTGTGTTAGTCGGTTCTGGCACCAGTCTACAGAAACAAACGGAGAGATAAGTTTAACTGAAGGTGATGAAGTTTTGGTTGAAGAGCCATGGTATAATGAGAATACGCTTCCTAGTTGTTTGGAAAATATAAGGAGAGATGACTTGGAGATAGTTCTTTTAGGGACTGGATCATCTCAGCCATCTAAATATCGCAATGTCACTTCTATCCATATCAATCTTTTCTCCAAAGGAGGTTTACTTTTAGATTGCGGTGAAGGAACCCTGGGACAGCTGAAAAGAAGATATGGTGTAGAGGGTGCTGATAATGCTGTGAGAGGTCTTAGCTGTATATGGATTTCTCATATTCATGCTGATCACCATACTGGGTTGGCAAGAATACTTACTCTACGACGTGATTTGTTGAAGGGGGTGCCTCACGAGCCATTACTTGTTGTTGGACCGAGAAAGCTTAAGTTCTTTTTGGATGCATACCAGAGACTCGAAAATTTAGATATGCAGTTCCTTGATTGTAAGCACACCACAGAAGCTTCTTTACTTGCTTTTGAGGGTGTTACTGAACCCAATAGGGATTACTCTTCTCCAGCAAGTCCAACCCGTTTTGAGGACATGAGCATTAAAAATACAGAGAGGCAATTAGCGGAGAGAGTTGATTCTACTCTATTTGCTAAAGGATCCCGTATGCAGAGCTATTGGAAGAGGCCGGGTAGTCCTATTGACAACAGTGTAGTTCCAATTGTAAAGAGCTTGCAAAAAGTGCTCAATGAAACTGGTTTGGAAGCCTTGATTAGTTTTCCTGTCATCCACTGTCCGCAGGCATTTGGTGTTGTCTTGCAAGCTTCAGAGAGATTGAATAGTGTTGGAAAAATGATTCCAGGTTGGAAGGTTGTATACTCAGGTGACACTAGGCCCTGTCCGGAACTTATAGACGCATCTCGTGGTGCAACAGTTCTAATACATGAGGCAACTTTTGAGGATGGCATGGTGGACGAGGCTGTAGCAAGAAACCATAGCACAACAAAGGAAGCCATAGAAGTTGGGAACTCTGCTGGAGTTTATCGTATCATCCTCACTCACTTCAGCCAGCGATATCCTAAAATCCCAGTACTTGATGATACACACATGCATAAGACATGCATTGGATTTGACATGATGAGTATTAACATTGCTGATTTGCCTGTGGCCCCAAAGGTACTTCCATACTTGAAACTGCTTTTTAGAGACGAGATGATTGTTGACGAGGAAGATGAGTTGATAGAGGCTGCAAGTGTGGCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0000963 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0031123 GO:0034414 GO:0034470 GO:0034641 GO:0034660 GO:0042779 GO:0042780 GO:0042781 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043628 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0072684 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140053 GO:0140098 GO:1901360 GO:1905267

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Lactamase_B_2 Lactamase_B_4

Protein Analysis

993

Amino Acids

109.8

Weight (kDa)

6.48

Isoelectric Point (pI)

46.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B_4	PF13691	132 - 188	3.4e-18	tRNase Z endonuclease
Anti-Pycsar_Apyc1	PF23023	616 - 695	1.1e-06	Anti-Pycsar protein Apyc1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16260 AT3G16260 AT3G16260
fragaria_vesca	FvH4_6g31990 FvH4_6g31990
malus_domestica	MD03G1197500.v1.1
prunus_persica	Prupe.3G063700_v2.0.a1
pyrus_communis	pycom03g14880
rosa_chinensis	RchiOBHm_Chr2g0139581
rosa_laevigata	RLG00000019820
rosa_multiflora	Rmu_sc0003427.1_g000004
rosa_roxburghii	Rroxscaffold_2G00105380
rosa_rugosa	Rorug02G0358400
rosa_samantha	Rh2AG408300 Rh2BG418900 Rh2CG394300 Rh2DG428000
rosa_wichuraiana	Rw2G033530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	548
AasI	GACNNNNNNGTC	1 cut(s)	971
Acc36I	ACCTGC	1 cut(s)	548
AccB1I	GGYRCC	3 cut(s)	1501, 1719, 2110
AccBSI	CCGCTC	1 cut(s)	210
AccI	GTMKAC	4 cut(s)	943, 1170, 1727, 2589
AccII	CGCG	1 cut(s)	180
AccIII	TCCGGA	2 cut(s)	1678, 2614
AciI	CCGC	9 cut(s)	120, 178, 210, 334, 1250, 1560, 1950, 2339, 2510
AclWI	GGATC	3 cut(s)	1876, 2367, 2380
AcsI	RAATTY	4 cut(s)	823, 1010, 1081, 2185
AcuI	CTGAAG	6 cut(s)	314, 405, 1466, 1779, 2523, 2761
AfaI	GTAC	3 cut(s)	865, 2805, 2892
AfiI	CCNNNNNNNGG	3 cut(s)	719, 1466, 2302
AflII	CTTAAG	1 cut(s)	2150
AflIII	ACRYGT	1 cut(s)	966
AgsI	TTSAA	8 cut(s)	277, 1103, 1521, 1539, 1781, 2104, 2551, 2905
AjiI	CACGTC	1 cut(s)	2093
AjnI	CCWGG	8 cut(s)	182, 568, 958, 1048, 1147, 1465, 1962, 2572
AjuI	GAANNNNNNNTTGG	2 cut(s)	1059, 1091
Alw21I	GWGCWC	2 cut(s)	1092, 2460
Alw26I	GTCTC	7 cut(s)	41, 68, 545, 1223, 1482, 2170, 2913
Alw44I	GTGCAC	1 cut(s)	1088
AlwI	GGATC	3 cut(s)	1876, 2367, 2380
AlwNI	CAGNNNCTG	1 cut(s)	554
Aor13HI	TCCGGA	2 cut(s)	1678, 2614
AoxI	GGCC	7 cut(s)	240, 314, 710, 1246, 2399, 2606, 2880
ApaLI	GTGCAC	1 cut(s)	1088
ApeKI	GCWGC	4 cut(s)	683, 1424, 1694, 2960
ApoI	RAATTY	4 cut(s)	823, 1010, 1081, 2185
Asp700I	GAANNNNTTC	1 cut(s)	885
AspLEI	GCGC	2 cut(s)	182, 345
AspS9I	GGNCC	8 cut(s)	629, 710, 1151, 1247, 1292, 2138, 2607, 2881
AsuC2I	CCSGG	1 cut(s)	2403
AsuHPI	GGTGA	8 cut(s)	76, 109, 611, 974, 1775, 1964, 2048, 2609
AvaII	GGWCC	4 cut(s)	629, 1151, 1292, 2138
BaeGI	GKGCMC	1 cut(s)	1092
BamHI	GGATCC	1 cut(s)	2372
BanI	GGYRCC	3 cut(s)	1501, 1719, 2110
BanII	GRGCYC	1 cut(s)	846
BarI	GAAGNNNNNNTAC	4 cut(s)	2217, 2249, 2573, 2605
BauI	CACGAG	3 cut(s)	537, 2117, 2634
Bbv12I	GWGCWC	2 cut(s)	1092, 2460
BbvCI	CCTCAGC	1 cut(s)	291
BbvI	GCAGC	4 cut(s)	670, 1436, 1706, 2947
BccI	CCATC	3 cut(s)	365, 1888, 2669
BciT130I	CCWGG	8 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2574
BciVI	GTATCC	2 cut(s)	394, 1216
BclI	TGATCA	3 cut(s)	520, 745, 2053
BcnI	CCSGG	1 cut(s)	2403
BcoDI	GTCTC	7 cut(s)	41, 68, 545, 1223, 1482, 2170, 2913
BfaI	CTAG	3 cut(s)	386, 1812, 2604
BfmI	CTRYAG	3 cut(s)	555, 1728, 2694
BfrI	CTTAAG	1 cut(s)	2150
BfuAI	ACCTGC	1 cut(s)	548
BfuI	GTATCC	2 cut(s)	394, 1216
BglI	GCCNNNNNGGC	1 cut(s)	248
BglII	AGATCT	1 cut(s)	637
BisI	GCNGC	4 cut(s)	684, 1425, 1695, 2961
BlsI	GCNGC	4 cut(s)	685, 1426, 1696, 2962
BmcAI	AGTACT	1 cut(s)	2805
Bme1390I	CCNGG	9 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2403, 2574
Bme18I	GGWCC	4 cut(s)	629, 1151, 1292, 2138
BmgBI	CACGTC	1 cut(s)	2093
BmgT120I	GGNCC	8 cut(s)	629, 710, 1151, 1247, 1292, 2138, 2607, 2881
BmrFI	CCNGG	9 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2403, 2574
BmrI	ACTGGG	3 cut(s)	849, 2073, 2795
BmsI	GCATC	5 cut(s)	646, 1307, 1681, 2155, 2639
BmuI	ACTGGG	3 cut(s)	849, 2073, 2795
BpmI	CTGGAG	4 cut(s)	486, 1449, 2265, 2769
Bpu10I	CCTNAGC	3 cut(s)	291, 1029, 1263
BpuEI	CTTGAG	1 cut(s)	1559
BpuMI	CCSGG	1 cut(s)	2403
BsaBI	GATNNNNATC	1 cut(s)	1397
BsaI	GGTCTC	1 cut(s)	1482
BsaJI	CCNNGG	7 cut(s)	243, 687, 1049, 1603, 1787, 1962, 1963
BsaWI	WCCGGW	2 cut(s)	1678, 2614
Bsc4I	CCNNNNNNNGG	3 cut(s)	719, 1466, 2302
Bse1I	ACTGG	9 cut(s)	397, 844, 861, 1206, 1723, 1870, 2068, 2473, 2801
Bse3DI	GCAATG	2 cut(s)	1900, 2862
Bse8I	GATNNNNATC	1 cut(s)	1397
BseAI	TCCGGA	2 cut(s)	1678, 2614
BseBI	CCWGG	8 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2574
BseDI	CCNNGG	7 cut(s)	243, 687, 1049, 1603, 1787, 1962, 1963
BseGI	GGATG	8 cut(s)	672, 748, 1117, 1338, 2170, 2499, 2680, 2763
BseJI	GATNNNNATC	1 cut(s)	1397
BseLI	CCNNNNNNNGG	3 cut(s)	719, 1466, 2302
BseMI	GCAATG	2 cut(s)	1900, 2862
BseNI	ACTGG	9 cut(s)	397, 844, 861, 1206, 1723, 1870, 2068, 2473, 2801
BseRI	GAGGAG	6 cut(s)	29, 58, 69, 228, 251, 1562
BseSI	GKGCMC	1 cut(s)	1092
BseXI	GCAGC	4 cut(s)	670, 1436, 1706, 2947
BseYI	CCCAGC	2 cut(s)	269, 1367
BsgI	GTGCAG	1 cut(s)	472
Bsh1236I	CGCG	1 cut(s)	180
BshFI	GGCC	7 cut(s)	242, 316, 712, 1248, 2401, 2608, 2882
BshNI	GGYRCC	3 cut(s)	1501, 1719, 2110
BsiHKAI	GWGCWC	2 cut(s)	1092, 2460
BsiSI	CCGG	3 cut(s)	1679, 2402, 2615
BslFI	GGGAC	4 cut(s)	403, 850, 1876, 1980
BslI	CCNNNNNNNGG	3 cut(s)	719, 1466, 2302
BsmAI	GTCTC	7 cut(s)	41, 68, 545, 1223, 1482, 2170, 2913
BsmBI	CGTCTC	2 cut(s)	41, 2913
BsmFI	GGGAC	4 cut(s)	403, 850, 1876, 1980
BsmI	GAATGC	1 cut(s)	1264
BsnI	GGCC	7 cut(s)	242, 316, 712, 1248, 2401, 2608, 2882
Bso31I	GGTCTC	1 cut(s)	1482
Bsp1286I	GDGCHC	3 cut(s)	846, 1092, 2460
Bsp13I	TCCGGA	2 cut(s)	1678, 2614
Bsp143I	GATC	6 cut(s)	520, 637, 745, 1868, 2053, 2372
Bsp19I	CCATGG	2 cut(s)	687, 1787
BspACI	CCGC	9 cut(s)	120, 178, 210, 334, 1250, 1560, 1950, 2339, 2510
BspANI	GGCC	7 cut(s)	242, 316, 712, 1248, 2401, 2608, 2882
BspEI	TCCGGA	2 cut(s)	1678, 2614
BspFNI	CGCG	1 cut(s)	180
BspHI	TCATGA	1 cut(s)	406
BspMAI	CTGCAG	1 cut(s)	559
BspMI	ACCTGC	1 cut(s)	548
BspPI	GGATC	3 cut(s)	1876, 2367, 2380
BspQI	GCTCTTC	2 cut(s)	280, 1776
BspT107I	GGYRCC	3 cut(s)	1501, 1719, 2110
BspTI	CTTAAG	1 cut(s)	2150
BspTNI	GGTCTC	1 cut(s)	1482
BsrBI	CCGCTC	1 cut(s)	210
BsrDI	GCAATG	2 cut(s)	1900, 2862
BsrI	ACTGG	9 cut(s)	397, 844, 861, 1206, 1723, 1870, 2068, 2473, 2801
BssECI	CCNNGG	7 cut(s)	243, 687, 1049, 1603, 1787, 1962, 1963
BssMI	GATC	6 cut(s)	520, 637, 745, 1868, 2053, 2372
BssNAI	GTATAC	1 cut(s)	2590
BssSI	CACGAG	3 cut(s)	537, 2117, 2634
BssT1I	CCWWGG	3 cut(s)	687, 1603, 1787
Bst1107I	GTATAC	1 cut(s)	2590
Bst2BI	CACGAG	3 cut(s)	537, 2117, 2634
Bst2UI	CCWGG	8 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2574
Bst4CI	ACNGT	7 cut(s)	152, 941, 1114, 1452, 2423, 2507, 2647
Bst6I	CTCTTC	8 cut(s)	47, 119, 280, 1331, 1485, 1776, 2281, 2390
BstAFI	CTTAAG	1 cut(s)	2150
BstAPI	GCANNNNNTGC	2 cut(s)	1421, 2455
BstC8I	GCNNGC	7 cut(s)	592, 1250, 1600, 2447, 2514, 2535, 2783
BstDSI	CCRYGG	3 cut(s)	243, 687, 1787
BstF5I	GGATG	8 cut(s)	672, 748, 1117, 1338, 2170, 2499, 2680, 2763
BstFNI	CGCG	1 cut(s)	180
BstHHI	GCGC	2 cut(s)	182, 345
BstKTI	GATC	6 cut(s)	523, 640, 748, 1871, 2056, 2375
BstMAI	GTCTC	7 cut(s)	41, 68, 545, 1223, 1482, 2170, 2913
BstMBI	GATC	6 cut(s)	520, 637, 745, 1868, 2053, 2372
BstNI	CCWGG	8 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2574
BstNSI	RCATGY	3 cut(s)	970, 2824, 2835
BstSCI	CCNGG	9 cut(s)	182, 568, 958, 1048, 1147, 1465, 1962, 2401, 2572
BstSFI	CTRYAG	3 cut(s)	555, 1728, 2694
BstSLI	GKGCMC	1 cut(s)	1092
BstUI	CGCG	1 cut(s)	180
BstV1I	GCAGC	4 cut(s)	670, 1436, 1706, 2947
BstX2I	RGATCY	2 cut(s)	637, 2372
BstXI	CCANNNNNNTGG	1 cut(s)	1473
BstYI	RGATCY	2 cut(s)	637, 2372
BstZ17I	GTATAC	1 cut(s)	2590
BsuI	GTATCC	2 cut(s)	394, 1216
BsuRI	GGCC	7 cut(s)	242, 316, 712, 1248, 2401, 2608, 2882
BtgI	CCRYGG	3 cut(s)	243, 687, 1787
BtrI	CACGTC	1 cut(s)	2093
BtsCI	GGATG	8 cut(s)	672, 748, 1117, 1338, 2170, 2499, 2680, 2763
BtsI	GCAGTG	2 cut(s)	264, 1512
BtsIMutI	CAGTG	6 cut(s)	264, 429, 1092, 1512, 2428, 2503
BveI	ACCTGC	1 cut(s)	548
Cac8I	GCNNGC	7 cut(s)	592, 1250, 1600, 2447, 2514, 2535, 2783
CaiI	CAGNNNCTG	1 cut(s)	554
CciI	TCATGA	1 cut(s)	406
CfoI	GCGC	2 cut(s)	182, 345
Cfr13I	GGNCC	8 cut(s)	629, 710, 1151, 1247, 1292, 2138, 2607, 2881
CseI	GACGC	2 cut(s)	1577, 2636
Csp6I	GTAC	3 cut(s)	864, 2804, 2891
CspCI	CAANNNNNGTGG	2 cut(s)	1617, 1652
CviQI	GTAC	3 cut(s)	864, 2804, 2891
DpnI	GATC	6 cut(s)	522, 639, 747, 1870, 2055, 2374
DpnII	GATC	6 cut(s)	520, 637, 745, 1868, 2053, 2372
DraI	TTTAAA	1 cut(s)	760
DrdI	GACNNNNNNGTC	1 cut(s)	971
DseDI	GACNNNNNNGTC	1 cut(s)	971
Eam1104I	CTCTTC	8 cut(s)	47, 119, 280, 1331, 1485, 1776, 2281, 2390
EarI	CTCTTC	8 cut(s)	47, 119, 280, 1331, 1485, 1776, 2281, 2390
Eco130I	CCWWGG	3 cut(s)	687, 1603, 1787
Eco24I	GRGCYC	1 cut(s)	846
Eco31I	GGTCTC	1 cut(s)	1482
Eco32I	GATATC	1 cut(s)	2789
Eco47I	GGWCC	4 cut(s)	629, 1151, 1292, 2138
Eco57I	CTGAAG	6 cut(s)	314, 405, 1466, 1779, 2523, 2761
EcoO109I	RGGNCCY	2 cut(s)	1292, 2607
EcoRII	CCWGG	8 cut(s)	182, 568, 958, 1048, 1147, 1465, 1962, 2572
EcoRV	GATATC	1 cut(s)	2789
EcoT14I	CCWWGG	3 cut(s)	687, 1603, 1787
EcoT22I	ATGCAT	3 cut(s)	2170, 2826, 2837
EcoT38I	GRGCYC	1 cut(s)	846
ErhI	CCWWGG	3 cut(s)	687, 1603, 1787
Esp3I	CGTCTC	2 cut(s)	41, 2913
FalI	AAGNNNNNCTT	1 cut(s)	2956
FaqI	GGGAC	4 cut(s)	403, 850, 1876, 1980
FauI	CCCGC	1 cut(s)	1257
FauNDI	CATATG	1 cut(s)	1594
FbaI	TGATCA	3 cut(s)	520, 745, 2053
FblI	GTMKAC	4 cut(s)	943, 1170, 1727, 2589
Fnu4HI	GCNGC	4 cut(s)	684, 1425, 1695, 2961
FokI	GGATG	8 cut(s)	659, 755, 1104, 1345, 2177, 2486, 2687, 2750
FriOI	GRGCYC	1 cut(s)	846
Fsp4HI	GCNGC	4 cut(s)	684, 1425, 1695, 2961
FspBI	CTAG	3 cut(s)	386, 1812, 2604
GlaI	GCGC	2 cut(s)	181, 344
GluI	GCNGC	4 cut(s)	684, 1425, 1695, 2961
GsaI	CCCAGC	2 cut(s)	273, 1371
GsuI	CTGGAG	4 cut(s)	486, 1449, 2265, 2769
HaeIII	GGCC	7 cut(s)	242, 316, 712, 1248, 2401, 2608, 2882
HapII	CCGG	3 cut(s)	1679, 2402, 2615
HgaI	GACGC	2 cut(s)	1577, 2636
HhaI	GCGC	2 cut(s)	182, 345
Hin6I	GCGC	2 cut(s)	180, 343
HinP1I	GCGC	2 cut(s)	180, 343
HincII	GTYRAC	2 cut(s)	582, 2935
HindII	GTYRAC	2 cut(s)	582, 2935
HindIII	AAGCTT	3 cut(s)	2147, 2226, 2535
HinfI	GANTC	9 cut(s)	715, 871, 1184, 1393, 1508, 1675, 2178, 2351, 2569
HpaI	GTTAAC	1 cut(s)	582
HpaII	CCGG	3 cut(s)	1679, 2402, 2615
HphI	GGTGA	8 cut(s)	76, 109, 611, 974, 1775, 1964, 2048, 2609
Hpy188III	TCNNGA	7 cut(s)	407, 890, 1064, 1679, 2117, 2615, 2976
Hpy99I	CGWCG	1 cut(s)	2094
HpyAV	CCTTC	9 cut(s)	250, 338, 429, 599, 1544, 1754, 1949, 2098, 2575
HpyCH4III	ACNGT	7 cut(s)	152, 941, 1114, 1452, 2423, 2507, 2647
HpyCH4IV	ACGT	2 cut(s)	1583, 2092
HpySE526I	ACGT	2 cut(s)	1583, 2092
HspAI	GCGC	2 cut(s)	180, 343
Kpn2I	TCCGGA	2 cut(s)	1678, 2614
Ksp22I	TGATCA	3 cut(s)	520, 745, 2053
KspAI	GTTAAC	1 cut(s)	582
Kzo9I	GATC	6 cut(s)	520, 637, 745, 1868, 2053, 2372
LguI	GCTCTTC	2 cut(s)	280, 1776
LmnI	GCTCC	4 cut(s)	350, 702, 849, 1468
Lsp1109I	GCAGC	4 cut(s)	670, 1436, 1706, 2947
LweI	GCATC	5 cut(s)	646, 1307, 1681, 2155, 2639
MaeI	CTAG	3 cut(s)	386, 1812, 2604
MaeII	ACGT	2 cut(s)	1583, 2092
MaeIII	GTNAC	7 cut(s)	22, 856, 962, 1270, 1897, 2251, 2597
MalI	GATC	6 cut(s)	522, 639, 747, 1870, 2055, 2374
MbiI	CCGCTC	1 cut(s)	210
MboI	GATC	6 cut(s)	520, 637, 745, 1868, 2053, 2372
MfeI	CAATTG	2 cut(s)	1155, 2433
MflI	RGATCY	2 cut(s)	637, 2372
MhlI	GDGCHC	3 cut(s)	846, 1092, 2460
MlyI	GAGTC	2 cut(s)	709, 2172
MmeI	TCCRAC	5 cut(s)	899, 2116, 2315, 2539, 2558
Mph1103I	ATGCAT	3 cut(s)	2170, 2826, 2837
MroI	TCCGGA	2 cut(s)	1678, 2614
MroXI	GAANNNNTTC	1 cut(s)	885
MslI	CAYNNNNRTG	2 cut(s)	1122, 2046
MspA1I	CMGCKG	3 cut(s)	334, 1562, 1972
MspCI	CTTAAG	1 cut(s)	2150
MspI	CCGG	3 cut(s)	1679, 2402, 2615
MspR9I	CCNGG	9 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2403, 2574
MunI	CAATTG	2 cut(s)	1155, 2433
Mva1269I	GAATGC	1 cut(s)	1264
MvaI	CCWGG	8 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2574
MvnI	CGCG	1 cut(s)	180
NciI	CCSGG	1 cut(s)	2403
NcoI	CCATGG	2 cut(s)	687, 1787
NdeI	CATATG	1 cut(s)	1594
NdeII	GATC	6 cut(s)	520, 637, 745, 1868, 2053, 2372
NmuCI	GTSAC	5 cut(s)	22, 962, 1270, 1897, 2597
NsiI	ATGCAT	3 cut(s)	2170, 2826, 2837
NspI	RCATGY	3 cut(s)	970, 2824, 2835
PagI	TCATGA	1 cut(s)	406
PaqCI	CACCTGC	1 cut(s)	548
PasI	CCCWGGG	1 cut(s)	1963
PciI	ACATGT	1 cut(s)	966
PciSI	GCTCTTC	2 cut(s)	280, 1776
PctI	GAATGC	1 cut(s)	1264
PdmI	GAANNNNTTC	1 cut(s)	885
PfeI	GAWTC	7 cut(s)	871, 1184, 1393, 1508, 1675, 2351, 2569
PkrI	GCNGC	4 cut(s)	685, 1426, 1696, 2962
PleI	GAGTC	2 cut(s)	709, 2172
PpsI	GAGTC	2 cut(s)	709, 2172
PpuMI	RGGWCCY	1 cut(s)	1292
PscI	ACATGT	1 cut(s)	966
Psp5II	RGGWCCY	1 cut(s)	1292
Psp6I	CCWGG	8 cut(s)	182, 568, 958, 1048, 1147, 1465, 1962, 2572
PspFI	CCCAGC	2 cut(s)	269, 1367
PspGI	CCWGG	8 cut(s)	182, 568, 958, 1048, 1147, 1465, 1962, 2572
PspPI	GGNCC	8 cut(s)	629, 710, 1151, 1247, 1292, 2138, 2607, 2881
PspPPI	RGGWCCY	1 cut(s)	1292
PstI	CTGCAG	1 cut(s)	559
PstNI	CAGNNNCTG	1 cut(s)	554
PsuI	RGATCY	2 cut(s)	637, 2372
PvuII	CAGCTG	1 cut(s)	1972
RsaI	GTAC	3 cut(s)	865, 2805, 2892
RsaNI	GTAC	3 cut(s)	864, 2804, 2891
RseI	CAYNNNNRTG	2 cut(s)	1122, 2046
SapI	GCTCTTC	2 cut(s)	280, 1776
SatI	GCNGC	4 cut(s)	684, 1425, 1695, 2961
Sau3AI	GATC	6 cut(s)	520, 637, 745, 1868, 2053, 2372
Sau96I	GGNCC	8 cut(s)	629, 710, 1151, 1247, 1292, 2138, 2607, 2881
ScaI	AGTACT	1 cut(s)	2805
SchI	GAGTC	2 cut(s)	709, 2172
ScrFI	CCNGG	9 cut(s)	184, 570, 960, 1050, 1149, 1467, 1964, 2403, 2574
SduI	GDGCHC	3 cut(s)	846, 1092, 2460
SfaNI	GCATC	5 cut(s)	646, 1307, 1681, 2155, 2639
SfcI	CTRYAG	3 cut(s)	555, 1728, 2694
SinI	GGWCC	4 cut(s)	629, 1151, 1292, 2138
SmiMI	CAYNNNNRTG	2 cut(s)	1122, 2046
SmlI	CTYRAG	2 cut(s)	1574, 2150
SmoI	CTYRAG	2 cut(s)	1574, 2150
SsiI	CCGC	9 cut(s)	120, 178, 210, 334, 1250, 1560, 1950, 2339, 2510
SspMI	CTAG	3 cut(s)	386, 1812, 2604
StyD4I	CCNGG	9 cut(s)	182, 568, 958, 1048, 1147, 1465, 1962, 2401, 2572
StyI	CCWWGG	3 cut(s)	687, 1603, 1787
TaaI	ACNGT	7 cut(s)	152, 941, 1114, 1452, 2423, 2507, 2647
TaiI	ACGT	2 cut(s)	1586, 2095
TaqI	TCGA	2 cut(s)	1429, 2181
TaqII	GACCGA	1 cut(s)	2155
TatI	WGTACW	2 cut(s)	863, 2803
TfiI	GAWTC	7 cut(s)	871, 1184, 1393, 1508, 1675, 2351, 2569
TscAI	CASTG	6 cut(s)	271, 429, 1092, 1519, 2428, 2510
TseFI	GTSAC	5 cut(s)	22, 962, 1270, 1897, 2597
TseI	GCWGC	4 cut(s)	683, 1424, 1694, 2960
Tsp45I	GTSAC	5 cut(s)	22, 962, 1270, 1897, 2597
TspGWI	ACGGA	5 cut(s)	23, 260, 621, 1196, 1755
TspRI	CASTG	6 cut(s)	271, 429, 1092, 1519, 2428, 2510
Vha464I	CTTAAG	1 cut(s)	2150
VneI	GTGCAC	1 cut(s)	1088
VpaK11BI	GGWCC	4 cut(s)	629, 1151, 1292, 2138
XapI	RAATTY	4 cut(s)	823, 1010, 1081, 2185
XceI	RCATGY	3 cut(s)	970, 2824, 2835
XmiI	GTMKAC	4 cut(s)	943, 1170, 1727, 2589
XmnI	GAANNNNTTC	1 cut(s)	885
XspI	CTAG	3 cut(s)	386, 1812, 2604
ZrmI	AGTACT	1 cut(s)	2805
Zsp2I	ATGCAT	3 cut(s)	2170, 2826, 2837

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.