Rorug02G0358400

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012976
Physical Location & Seq
Reverse (-)
45259630 .. 45271564
11935 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0358400.1

Sequence Viewer

Length: 1320 bp
ATGGCGATCAACGTGAGCGTGCGGGAGTCGACGTTTGTGAAGCCATCGGAGGAGACGCCTAGGCGTGCGCTTTGGATTTCCAACCTGGACGCCGTTGTGCTCAACACTCACATGGGCAGTGTTTACTTCTACAGGAGGAAAACCGGCACGCCGAGTAACTTCTTCGACCCAGAAGTGCTCAAGGAAGCCCTCAGCAAGGCACTCGTGTCTTTCTACCCCATGGCAGGCCGGCTTAAGAAGAAAATCCACGAAGGCCGCAGCCGTGTCGAAATCGATTGCAATGGGGAGGGGGTGCTCTTTGTTGTAGCGGAGACCAGCTCTGTGGTGGATGACTTTGGCGACTTTGCTCCCACTCTAGAGTTCCGGCGGCTCATCCCTGCCGTTGATTACTCCGCTGGGATATCATCTTATCCCCTTTTGGTCTTACAGGTTACATACTTCAAATGTGGTGGAGTGTCACTTGGTGTTGGCATGGAACACCGGGTAGCAGATGGATATTCTGGTCTCCATTTCATAAATACATGGTCTGATATCACTCGTGGCCTCAACCTCACAATTCCACCATTCATCGACCGCACATTACTGCGTGCTCGAGATCCGCCCCGGCCTGCATTCCCCCACATCGAATACCAACCCGGTCCGTCTATGAAAACTCCTCTTGATGCTGCTGAGATGACACCAACAATGGTCTCCATTTTCAAGCTGACACCAGACCAGCTCAACACCTTGAAAGCTAAGTTTAAACAAGATGGGAACTACACGATCAAGTTCAGCTTATATGAAATGTTGGCAGGTCATATATGGCGATGTGCATGCAAGGCACGCCGACTCCCCGATGATCAAGAGACCAAACTGCATATCGCCACTGATGGAAGGTCCAGACTGAAGCCTCCACTCCCTCCCGGTTACTTTGGCAACGTGATTTTCTCAGCCGGGCCATTCGCTACAGCAGGGGATCTCCAATCAAAACCGATATGGTATGCTTCAAGCTGTATACAAAATACTTTGGCACGTATGGACGATGAGTATCTTAGATCAGCAATCGACTATCTTGAGCTTCAGCCGGATCTTTCGGCACTTGTTCGCGGGGCTCATAGTTTTCGCTGCCCAAAAATGGGGATTACTAGTTGGGCTAGGCTGCCGATCCATGATGCTGATTTCGGCTGGGGTCGACCCATTTTCATGGGACCTGGAGGGATTGCATTTGAAGGCTTGTGCTTCATTTTACCGAGTGCAAGTAAAGACGGGAGCTTATCAGTGGCCATTTCACTCCAATCTGAACATATGAAATCGTTTTCGCAGTTCTTATATGACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

439

Amino Acids

48.87

Weight (kDa)

8.35

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 4 - 434 2e-110 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012976)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16260 AT3G16260 AT3G16260
fragaria_vesca FvH4_6g31990 FvH4_6g31990
malus_domestica MD03G1197500.v1.1
prunus_persica Prupe.3G063700_v2.0.a1
pyrus_communis pycom03g14880
rosa_chinensis RchiOBHm_Chr2g0139581
rosa_laevigata RLG00000019820
rosa_multiflora Rmu_sc0003427.1_g000004
rosa_roxburghii Rroxscaffold_2G00105380
rosa_rugosa Rorug02G0358400
rosa_samantha Rh2AG408300 Rh2BG418900 Rh2CG394300 Rh2DG428000
rosa_wichuraiana Rw2G033530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 782
AccI GTMKAC 3 cut(s) 29, 994, 1171
AccII CGCG 1 cut(s) 1086
AciI CCGC 8 cut(s) 22, 256, 308, 367, 393, 574, 599, 1086
AclWI GGATC 4 cut(s) 590, 963, 1074, 1138
AcoI YGGCCR 1 cut(s) 1260
AcuI CTGAAG 2 cut(s) 905, 1043
AcyI GRCGYC 2 cut(s) 56, 90
AdeI CACNNNGTG 1 cut(s) 464
AfiI CCNNNNNNNGG 4 cut(s) 196, 224, 1114, 1115
AflII CTTAAG 1 cut(s) 233
AgsI TTSAA 5 cut(s) 442, 700, 730, 987, 1208
AhlI ACTAGT 1 cut(s) 1124
AjnI CCWGG 2 cut(s) 84, 1189
AluBI AGCT 8 cut(s) 318, 703, 718, 734, 774, 990, 1057, 1251
AluI AGCT 8 cut(s) 318, 703, 718, 734, 774, 990, 1057, 1251
Alw21I GWGCWC 4 cut(s) 102, 180, 297, 592
Alw26I GTCTC 5 cut(s) 47, 305, 509, 694, 839
AlwI GGATC 4 cut(s) 590, 963, 1074, 1138
Ama87I CYCGRG 1 cut(s) 591
AoxI GGCC 6 cut(s) 226, 253, 541, 605, 935, 1260
ApeKI GCWGC 4 cut(s) 258, 665, 1104, 1138
AspA2I CCTAGG 1 cut(s) 59
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 4 cut(s) 638, 876, 935, 1187
AsuC2I CCSGG 5 cut(s) 482, 604, 636, 903, 934
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 3 cut(s) 638, 876, 1187
AvrII CCTAGG 1 cut(s) 59
BalI TGGCCA 1 cut(s) 1262
BanII GRGCYC 1 cut(s) 1093
BauI CACGAG 2 cut(s) 203, 537
Bbv12I GWGCWC 4 cut(s) 102, 180, 297, 592
BbvCI CCTCAGC 1 cut(s) 191
BbvI GCAGC 4 cut(s) 270, 652, 1091, 1125
BccI CCATC 4 cut(s) 52, 485, 743, 863
BceAI ACGGC 3 cut(s) 77, 246, 365
BcgI CGANNNNNNTGC 6 cut(s) 184, 218, 247, 281, 795, 829
BciT130I CCWGG 2 cut(s) 86, 1191
BclI TGATCA 1 cut(s) 838
BcnI CCSGG 5 cut(s) 482, 604, 636, 903, 934
BcoDI GTCTC 5 cut(s) 47, 305, 509, 694, 839
BcuI ACTAGT 1 cut(s) 1124
BfaI CTAG 4 cut(s) 60, 356, 1125, 1134
BfmI CTRYAG 2 cut(s) 130, 945
BfrI CTTAAG 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 782
BisI GCNGC 6 cut(s) 256, 259, 368, 666, 1105, 1139
BlnI CCTAGG 1 cut(s) 59
BlsI GCNGC 6 cut(s) 257, 260, 369, 667, 1106, 1140
Bme1390I CCNGG 7 cut(s) 86, 482, 604, 636, 903, 934, 1191
Bme18I GGWCC 3 cut(s) 638, 876, 1187
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 4 cut(s) 638, 876, 935, 1187
BmiI GGNNCC 1 cut(s) 1188
BmrFI CCNGG 7 cut(s) 86, 482, 604, 636, 903, 934, 1191
BmsI GCATC 2 cut(s) 652, 1141
BplI GAGNNNNNCTC 2 cut(s) 302, 334
BpmI CTGGAG 1 cut(s) 1212
Bpu10I CCTNAGC 1 cut(s) 191
BpuEI CTTGAG 2 cut(s) 164, 1073
BpuMI CCSGG 5 cut(s) 482, 604, 636, 903, 934
Bsa29I ATCGAT 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 1013
BsaBI GATNNNNATC 1 cut(s) 1026
BsaHI GRCGYC 2 cut(s) 56, 90
BsaI GGTCTC 4 cut(s) 305, 509, 694, 839
BsaJI CCNNGG 3 cut(s) 59, 219, 602
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 813, 843
Bsc4I CCNNNNNNNGG 4 cut(s) 196, 224, 1114, 1115
Bse118I RCCGGY 2 cut(s) 143, 228
Bse3DI GCAATG 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 1026
BseBI CCWGG 2 cut(s) 86, 1191
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 3 cut(s) 59, 219, 602
BseGI GGATG 2 cut(s) 334, 372
BseJI GATNNNNATC 1 cut(s) 1026
BseLI CCNNNNNNNGG 4 cut(s) 196, 224, 1114, 1115
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 3 cut(s) 205, 660, 942
BseRI GAGGAG 2 cut(s) 65, 645
BseXI GCAGC 4 cut(s) 270, 652, 1091, 1125
BseYI CCCAGC 2 cut(s) 395, 1164
Bsh1236I CGCG 1 cut(s) 1086
Bsh1285I CGRYCG 1 cut(s) 574
BshFI GGCC 6 cut(s) 228, 255, 543, 607, 937, 1262
BshVI ATCGAT 1 cut(s) 273
BsiEI CGRYCG 1 cut(s) 574
BsiHKAI GWGCWC 4 cut(s) 102, 180, 297, 592
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 9 cut(s) 144, 229, 364, 481, 604, 636, 903, 933, 1064
BslFI GGGAC 1 cut(s) 1200
BslI CCNNNNNNNGG 4 cut(s) 196, 224, 1114, 1115
BsmAI GTCTC 5 cut(s) 47, 305, 509, 694, 839
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 1200
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 6 cut(s) 228, 255, 543, 607, 937, 1262
Bso31I GGTCTC 4 cut(s) 305, 509, 694, 839
BsoBI CYCGRG 1 cut(s) 591
Bsp1286I GDGCHC 5 cut(s) 102, 180, 297, 592, 1093
Bsp143I GATC 8 cut(s) 6, 595, 762, 838, 955, 1034, 1066, 1143
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 8 cut(s) 22, 256, 308, 367, 393, 574, 599, 1086
BspANI GGCC 6 cut(s) 228, 255, 543, 607, 937, 1262
BspCNI CTCAG 3 cut(s) 204, 661, 941
BspDI ATCGAT 1 cut(s) 273
BspFNI CGCG 1 cut(s) 1086
BspLI GGNNCC 1 cut(s) 1188
BspMI ACCTGC 1 cut(s) 782
BspPI GGATC 4 cut(s) 590, 963, 1074, 1138
BspTI CTTAAG 1 cut(s) 233
BspTNI GGTCTC 4 cut(s) 305, 509, 694, 839
BsrDI GCAATG 1 cut(s) 286
BsrFI RCCGGY 2 cut(s) 143, 228
BssAI RCCGGY 2 cut(s) 143, 228
BssECI CCNNGG 3 cut(s) 59, 219, 602
BssMI GATC 8 cut(s) 6, 595, 762, 838, 955, 1034, 1066, 1143
BssNAI GTATAC 1 cut(s) 995
BssNI GRCGYC 2 cut(s) 56, 90
BssSI CACGAG 2 cut(s) 203, 537
BssT1I CCWWGG 2 cut(s) 59, 219
Bst1107I GTATAC 1 cut(s) 995
Bst2BI CACGAG 2 cut(s) 203, 537
Bst2UI CCWGG 2 cut(s) 86, 1191
BstACI GRCGYC 2 cut(s) 56, 90
BstAFI CTTAAG 1 cut(s) 233
BstBAI YACGTR 1 cut(s) 1013
BstC8I GCNNGC 9 cut(s) 20, 66, 149, 226, 230, 588, 609, 814, 823
BstDEI CTNAG 5 cut(s) 191, 669, 735, 928, 1031
BstDSI CCRYGG 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 194
BstF5I GGATG 2 cut(s) 334, 372
BstFNI CGCG 1 cut(s) 1086
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 8 cut(s) 9, 598, 765, 841, 958, 1037, 1069, 1146
BstMAI GTCTC 5 cut(s) 47, 305, 509, 694, 839
BstMBI GATC 8 cut(s) 6, 595, 762, 838, 955, 1034, 1066, 1143
BstMCI CGRYCG 1 cut(s) 574
BstMWI GCNNNNNNNGC 2 cut(s) 818, 822
BstNI CCWGG 2 cut(s) 86, 1191
BstNSI RCATGY 1 cut(s) 816
BstSCI CCNGG 7 cut(s) 84, 480, 602, 634, 901, 932, 1189
BstSFI CTRYAG 2 cut(s) 130, 945
BstUI CGCG 1 cut(s) 1086
BstV1I GCAGC 4 cut(s) 270, 652, 1091, 1125
BstX2I RGATCY 3 cut(s) 595, 955, 1066
BstXI CCANNNNNNTGG 2 cut(s) 322, 1183
BstYI RGATCY 3 cut(s) 595, 955, 1066
BstZ17I GTATAC 1 cut(s) 995
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 6 cut(s) 228, 255, 543, 607, 937, 1262
BsuTUI ATCGAT 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 219
BtgZI GCGATG 1 cut(s) 820
BtsCI GGATG 2 cut(s) 334, 372
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 3 cut(s) 124, 864, 1263
BveI ACCTGC 1 cut(s) 782
Cac8I GCNNGC 9 cut(s) 20, 66, 149, 226, 230, 588, 609, 814, 823
CfoI GCGC 1 cut(s) 70
Cfr10I RCCGGY 2 cut(s) 143, 228
Cfr13I GGNCC 4 cut(s) 638, 876, 935, 1187
ClaI ATCGAT 1 cut(s) 273
CpoI CGGWCCG 1 cut(s) 638
CseI GACGC 2 cut(s) 64, 98
CspCI CAANNNNNGTGG 2 cut(s) 430, 465
CspI CGGWCCG 1 cut(s) 638
CviAII CATG 7 cut(s) 112, 220, 472, 522, 813, 1148, 1183
DdeI CTNAG 5 cut(s) 191, 669, 735, 928, 1031
DpnI GATC 8 cut(s) 8, 597, 764, 840, 957, 1036, 1068, 1145
DpnII GATC 8 cut(s) 6, 595, 762, 838, 955, 1034, 1066, 1143
DraI TTTAAA 1 cut(s) 742
DraIII CACNNNGTG 1 cut(s) 464
EaeI YGGCCR 1 cut(s) 1260
EciI GGCGGA 1 cut(s) 588
Eco130I CCWWGG 2 cut(s) 59, 219
Eco24I GRGCYC 1 cut(s) 1093
Eco31I GGTCTC 4 cut(s) 305, 509, 694, 839
Eco32I GATATC 2 cut(s) 402, 532
Eco47I GGWCC 3 cut(s) 638, 876, 1187
Eco57I CTGAAG 2 cut(s) 905, 1043
Eco88I CYCGRG 1 cut(s) 591
EcoNI CCTNNNNNAGG 1 cut(s) 194
EcoO109I RGGNCCY 1 cut(s) 1187
EcoRII CCWGG 2 cut(s) 84, 1189
EcoRV GATATC 2 cut(s) 402, 532
EcoT14I CCWWGG 2 cut(s) 59, 219
EcoT38I GRGCYC 1 cut(s) 1093
ErhI CCWWGG 2 cut(s) 59, 219
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 7 cut(s) 115, 223, 475, 525, 816, 1151, 1186
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FaqI GGGAC 1 cut(s) 1200
FatI CATG 7 cut(s) 111, 219, 471, 521, 812, 1147, 1182
FauI CCCGC 2 cut(s) 15, 1079
FauNDI CATATG 1 cut(s) 1284
FbaI TGATCA 1 cut(s) 838
FblI GTMKAC 3 cut(s) 29, 994, 1171
Fnu4HI GCNGC 6 cut(s) 256, 259, 368, 666, 1105, 1139
FokI GGATG 2 cut(s) 341, 359
FriOI GRGCYC 1 cut(s) 1093
Fsp4HI GCNGC 6 cut(s) 256, 259, 368, 666, 1105, 1139
FspBI CTAG 4 cut(s) 60, 356, 1125, 1134
GlaI GCGC 1 cut(s) 69
GluI GCNGC 6 cut(s) 256, 259, 368, 666, 1105, 1139
GsaI CCCAGC 2 cut(s) 399, 1168
GsuI CTGGAG 1 cut(s) 1212
HaeIII GGCC 6 cut(s) 228, 255, 543, 607, 937, 1262
HapII CCGG 9 cut(s) 144, 229, 364, 481, 604, 636, 903, 933, 1064
HgaI GACGC 2 cut(s) 64, 98
HhaI GCGC 1 cut(s) 70
Hin1I GRCGYC 2 cut(s) 56, 90
Hin1II CATG 7 cut(s) 115, 223, 475, 525, 816, 1151, 1186
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HincII GTYRAC 2 cut(s) 30, 1172
HindII GTYRAC 2 cut(s) 30, 1172
HinfI GANTC 2 cut(s) 26, 828
HpaII CCGG 9 cut(s) 144, 229, 364, 481, 604, 636, 903, 933, 1064
Hpy166II GTNNAC 4 cut(s) 30, 124, 995, 1172
Hpy188I TCNGA 3 cut(s) 49, 529, 1279
Hpy188III TCNNGA 6 cut(s) 356, 593, 659, 842, 879, 1052
Hpy8I GTNNAC 4 cut(s) 30, 124, 995, 1172
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 3 cut(s) 245, 867, 1202
HpyCH4IV ACGT 4 cut(s) 12, 32, 918, 1012
HpyCH4V TGCA 7 cut(s) 279, 611, 812, 816, 856, 1202, 1235
HpyF10VI GCNNNNNNNGC 2 cut(s) 818, 822
HpyF3I CTNAG 5 cut(s) 191, 669, 735, 928, 1031
HpySE526I ACGT 4 cut(s) 12, 32, 918, 1012
Hsp92I GRCGYC 2 cut(s) 56, 90
Hsp92II CATG 7 cut(s) 115, 223, 475, 525, 816, 1151, 1186
HspAI GCGC 1 cut(s) 68
KroI GCCGGC 1 cut(s) 228
KroNI GCCGGC 1 cut(s) 230
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 8 cut(s) 6, 595, 762, 838, 955, 1034, 1066, 1143
LmnI GCTCC 2 cut(s) 352, 1248
Lsp1109I GCAGC 4 cut(s) 270, 652, 1091, 1125
LweI GCATC 2 cut(s) 652, 1141
MaeI CTAG 4 cut(s) 60, 356, 1125, 1134
MaeII ACGT 4 cut(s) 12, 32, 918, 1012
MaeIII GTNAC 4 cut(s) 155, 430, 456, 905
MalI GATC 8 cut(s) 8, 597, 764, 840, 957, 1036, 1068, 1145
MboI GATC 8 cut(s) 6, 595, 762, 838, 955, 1034, 1066, 1143
MboII GAAGA 2 cut(s) 154, 250
MflI RGATCY 3 cut(s) 595, 955, 1066
MhlI GDGCHC 5 cut(s) 102, 180, 297, 592, 1093
MlsI TGGCCA 1 cut(s) 1262
MluCI AATT 1 cut(s) 555
MluNI TGGCCA 1 cut(s) 1262
MlyI GAGTC 2 cut(s) 35, 822
MmeI TCCRAC 1 cut(s) 105
Mox20I TGGCCA 1 cut(s) 1262
MroNI GCCGGC 1 cut(s) 228
MscI TGGCCA 1 cut(s) 1262
MseI TTAA 2 cut(s) 234, 741
MslI CAYNNNNRTG 2 cut(s) 110, 1181
Msp20I TGGCCA 1 cut(s) 1262
MspA1I CMGCKG 1 cut(s) 395
MspCI CTTAAG 1 cut(s) 233
MspI CCGG 9 cut(s) 144, 229, 364, 481, 604, 636, 903, 933, 1064
MspR9I CCNGG 7 cut(s) 86, 482, 604, 636, 903, 934, 1191
MssI GTTTAAAC 1 cut(s) 742
Mva1269I GAATGC 1 cut(s) 611
MvaI CCWGG 2 cut(s) 86, 1191
MvnI CGCG 1 cut(s) 1086
MwoI GCNNNNNNNGC 2 cut(s) 818, 822
NaeI GCCGGC 1 cut(s) 230
NciI CCSGG 5 cut(s) 482, 604, 636, 903, 934
NcoI CCATGG 1 cut(s) 219
NdeI CATATG 1 cut(s) 1284
NdeII GATC 8 cut(s) 6, 595, 762, 838, 955, 1034, 1066, 1143
NgoMIV GCCGGC 1 cut(s) 228
NlaIII CATG 7 cut(s) 115, 223, 475, 525, 816, 1151, 1186
NlaIV GGNNCC 1 cut(s) 1188
NmeAIII GCCGAG 1 cut(s) 177
NmuCI GTSAC 1 cut(s) 456
NspI RCATGY 1 cut(s) 816
PaeI GCATGC 1 cut(s) 816
PaeR7I CTCGAG 1 cut(s) 591
PctI GAATGC 1 cut(s) 611
PdiI GCCGGC 1 cut(s) 230
PkrI GCNGC 6 cut(s) 257, 260, 369, 667, 1106, 1140
PleI GAGTC 2 cut(s) 34, 822
PmeI GTTTAAAC 1 cut(s) 742
PpsI GAGTC 2 cut(s) 34, 822
Ppu21I YACGTR 1 cut(s) 1013
PpuMI RGGWCCY 1 cut(s) 1187
Psp5II RGGWCCY 1 cut(s) 1187
Psp6I CCWGG 2 cut(s) 84, 1189
PspFI CCCAGC 2 cut(s) 395, 1164
PspGI CCWGG 2 cut(s) 84, 1189
PspN4I GGNNCC 1 cut(s) 1188
PspPI GGNCC 4 cut(s) 638, 876, 935, 1187
PspPPI RGGWCCY 1 cut(s) 1187
PsuI RGATCY 3 cut(s) 595, 955, 1066
RseI CAYNNNNRTG 2 cut(s) 110, 1181
Rsr2I CGGWCCG 1 cut(s) 638
RsrII CGGWCCG 1 cut(s) 638
SalI GTCGAC 2 cut(s) 28, 1170
SaqAI TTAA 2 cut(s) 234, 741
SatI GCNGC 6 cut(s) 256, 259, 368, 666, 1105, 1139
Sau3AI GATC 8 cut(s) 6, 595, 762, 838, 955, 1034, 1066, 1143
Sau96I GGNCC 4 cut(s) 638, 876, 935, 1187
SchI GAGTC 2 cut(s) 35, 822
ScrFI CCNGG 7 cut(s) 86, 482, 604, 636, 903, 934, 1191
SduI GDGCHC 5 cut(s) 102, 180, 297, 592, 1093
SfaNI GCATC 2 cut(s) 652, 1141
SfcI CTRYAG 2 cut(s) 130, 945
Sfr274I CTCGAG 1 cut(s) 591
SinI GGWCC 3 cut(s) 638, 876, 1187
SlaI CTCGAG 1 cut(s) 591
SmiMI CAYNNNNRTG 2 cut(s) 110, 1181
SmlI CTYRAG 4 cut(s) 179, 233, 591, 1052
SmoI CTYRAG 4 cut(s) 179, 233, 591, 1052
SpeI ACTAGT 1 cut(s) 1124
SphI GCATGC 1 cut(s) 816
Sse9I AATT 1 cut(s) 555
SsiI CCGC 8 cut(s) 22, 256, 308, 367, 393, 574, 599, 1086
SspMI CTAG 4 cut(s) 60, 356, 1125, 1134
StyD4I CCNGG 7 cut(s) 84, 480, 602, 634, 901, 932, 1189
StyI CCWWGG 2 cut(s) 59, 219
TaiI ACGT 4 cut(s) 15, 35, 921, 1015
TaqI TCGA 9 cut(s) 29, 165, 267, 273, 570, 592, 624, 1044, 1171
TasI AATT 1 cut(s) 555
TauI GCSGC 2 cut(s) 258, 370
Tru1I TTAA 2 cut(s) 234, 741
Tru9I TTAA 2 cut(s) 234, 741
TscAI CASTG 3 cut(s) 124, 871, 1263
TseFI GTSAC 1 cut(s) 456
TseI GCWGC 4 cut(s) 258, 665, 1104, 1138
Tsp45I GTSAC 1 cut(s) 456
TspDTI ATGAA 7 cut(s) 502, 556, 662, 795, 1171, 1210, 1301
TspGWI ACGGA 1 cut(s) 630
TspRI CASTG 3 cut(s) 124, 871, 1263
Vha464I CTTAAG 1 cut(s) 233
VpaK11BI GGWCC 3 cut(s) 638, 876, 1187
XagI CCTNNNNNAGG 1 cut(s) 194
XbaI TCTAGA 1 cut(s) 355
XceI RCATGY 1 cut(s) 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 591
XmaJI CCTAGG 1 cut(s) 59
XmiI GTMKAC 3 cut(s) 29, 994, 1171
XspI CTAG 4 cut(s) 60, 356, 1125, 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.