Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0012976

Physical Location & Seq

Genomic Coordinates Reverse (-)

54852585 .. 54858239

Gene Structure 5655 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G033530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2910 bp

ATGCAAGTCTCAAACCTTCGTCTCCTCTTGTTTAGCCCCTTCGTCCCTCCTCTCTCACTCTCACCCCTTAAACCCTCTTTCTCTCTCTTCCCGAAATCAAAGCCTTACTCTCTCCTCACCGCCCTCGCCTCCGCCTCCTCCTCCCACCCAAAACGACGCCGTCCCATTCCCTACAGGTCTCCAGGACCCAGAAGCAAAAGCACGCTCAGACACCGAGACAAAGCAATGGAGGATAAGACCGAGTCCGTTTCGTTCAACAAGAGAAGAGCGGAGGGTCACGACAAGAATGAAAGACCCAAGAAGAATCTCCAGAGGAAAGTCCGCGCTCTCAATCCTACCAATACCATAGCTTATGTCCAGTTTCTAGGGACTGGAATGGATACTCTGGATACATCTCCTTCAGTGTTGCTATTCTTTGACAAGCAAAGATTTATTTTCAATGCTGGAGAGGGATTGCAAAGGTTCTGCACAGAGCATAAGATTAAGTTGTCAAAGATAGATCACATATTTCTCTCTCGTGTCTGCTCAGAGACTGCCGGTGGAATTCCAGGTCTGCTGTTGACTTTGGCTGGCATGGGTGAAGAAGGAATGTCTGTTAATGTGTGGGGTCCATCAGATCTGAAGTATTTGGTTGATGCCATGAAGTGTTTCATTCCAAATGCTGCCATGGTTCACACTAGGAGCTTTGGGCCCACACATGGTTCTGTGGGGGGTCCTATGGCTGATGAAACTCAGTTTACAGATCCCATTGTTCTTGTTGACGACGAAGTTGTCAAATTATCAGCCATTCTTCTACGACCAAGTTTCTTGGAAGGGTCTCAGCTCCCGAATGGAGTACCCATCATGCAGAATCCTAGAGAAAAGGTCATTGATGATAGTATGGATCATGCTTCAGAACCCATTAGTCCAAATGGCAGAAGTATGCTTACTGGAAAGCCTGGTGACATGTCCGTAATCTATGTCTGTGAATTACCTGACATCAAAGGGAAATTTGACCCTGCGAAAGCTGTGGCTTTAGGTCTTAGACCAGGGTCAAAATATCGGGAACTGCAACTTGGAAACTCGGTGAAGTCAGATTACCAGAACATCATGGTTCATCCAAGTGATGTAATGGGGCCCTCTGTTCCTGGTCCTATTGTACTCCTCGTCGACTGCCCAACTGAATCTCATTTGCAGGAGTTGTTATCCGTACAATGTCTCAGTAGCTATTATGCAGATTTCTCAGGCCCACCTGAGAATGCTAAGGTTGTCACTTGTGTGATTCATTTGAGTCCTGCATCTTTAATAAGCAGTTCTAATTACCAGAGCTGGATGAAAAGATTTGGTTCAGCCCAACATATCATGGCTGGACATGAAAGGAAGAATGTGGAGATTCCAATTCTAAAGTCAAGTGCAAGAATTGCAGCTCGACTCAACTATCTATGTCCACAGTTCTTTCCAGCTCCAGGTTTCTGGTCTCTTCAGCAGAGTGATTGCTTAGCACCAGAGTCCACTGCTTCTAGTGAGGGTTCTGTTTGTGAAAGCATTTCCGCTGAAAACCTCCTCAAGTTCACTTTGCGGCCATATGCTCATCTTGGATTAGATCGGTCTGTTATTCCAAGTAAGGTGGCTGCCGCAGATGTTATTCATGACTTGCTTTCAGAGAATCCAGAGGTTGTAGATGCTGCCCAATGTGTTAGCCAGTTCTGGCGTCAGTCCACAGAAACAAGGGAAGAGGTAAATTTTGTGCAAGAAGATACAGTAATGGTTGAAGAGCCATGGTTTAGTGAGAATACACTTCCTAGTTGCTTGGAGAATATAAGGAGGGATGATTTGGAGATAGTTCTTCTAGGAACTGGTTCTTCTCAGCCATCTAAATATCGCAATGTCAGTGCTATCCATATCAACCTTTTCTCCAATGGAGGTTTGCTTTTAGATTGTGGCGAAGGAACCCTAGGACAGCTGAAAAGAAGATATGGTGTAGAGGGTGCTGATAATGCTGTGAGAGGTCTTCGATGTATATGGATTTCTCATATTCATGCTGATCACCATACGGGATTGGCAAGAATACTTGCTCTGCGACGTACTTTGTTGAAGGGAGTGCCTCATGAACCACTACTTGTAGTTGGACCAAGACAGCTAAAGAGATATTTGGATGCATACCAAAGACTTGAAGATTTAGATATGCAGTTCCTTGATTGTAAGCACACCACAGAAGCGTCTTTAAATGCTTTTGAGGGTGCTACTGAATTAAATAAACGTCACTCCTCTCCCGGAAAAGATAGGCAACAGAAAGTTGATTCTACTCTATTTGCTAAAGGATCTCGTATGGAGAGCTATTGGAAGAGACCTAATAGTCCAGTTGACGATGGTGTGGTTTTTCCAATTCTAAAGAGTTTGCAAAAATTGCTTAGCGAAGCTGGACTGGAAGCCTTGATTAGTGTTCCTGTCATGCACTGCCCACAGGCATTTGGTGTTGTCTTGAAGGCTTCAGAGAGAATGAATAGTGTTGGAAAAGTGATACCAGGGTGGAAGCTTGTGTACTCTGGTGATACTAGGCCCTGCCCGGCACTTACAGAAGCATCTCGTGGTGCAACAATTCTTATACATGAGGCAACGTTCGAGGATGGCATGGTGGATGAGGCCATAGCAAGAAACCACAGCACAACCAAGGAAGCCATAGAAGTAGGGAACTCTGCAGGTGTTTATCGCATCATCCTTACCCACTTCAGCCAGCGATACCCAAAAATCCCAGTATTTGACGAGACACACATGCATAAGACATGCATTGCATTTGACCTGATGAGTATTAACATAGCTGATTTGCCAGTTCTACCAAAAGTACTTCCATACTTGAAAATGCTCTTTAAAAACGAGATGATAGTCGATGAATCAGATGAGATGTTCGATGCTGCAAGTGTTGCTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0000963 GO:0003674 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006399 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0016891 GO:0016893 GO:0031123 GO:0034414 GO:0034470 GO:0034641 GO:0034660 GO:0042779 GO:0042780 GO:0042781 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043628 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0072684 GO:0090304 GO:0090305 GO:0090501 GO:0090502 GO:0140053 GO:0140098 GO:1901360 GO:1905267

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Lactamase_B_2 Lactamase_B_4

Protein Analysis

969

Amino Acids

107.06

Weight (kDa)

7.0

Isoelectric Point (pI)

49.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B_4	PF13691	125 - 181	4.4e-18	tRNase Z endonuclease
Anti-Pycsar_Apyc1	PF23023	606 - 686	1.2e-07	Anti-Pycsar protein Apyc1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012976)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G16260 AT3G16260 AT3G16260
fragaria_vesca	FvH4_6g31990 FvH4_6g31990
malus_domestica	MD03G1197500.v1.1
prunus_persica	Prupe.3G063700_v2.0.a1
pyrus_communis	pycom03g14880
rosa_chinensis	RchiOBHm_Chr2g0139581
rosa_laevigata	RLG00000019820
rosa_multiflora	Rmu_sc0003427.1_g000004
rosa_roxburghii	Rroxscaffold_2G00105380
rosa_rugosa	Rorug02G0358400
rosa_samantha	Rh2AG408300 Rh2BG418900 Rh2CG394300 Rh2DG428000
rosa_wichuraiana	Rw2G033530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2669
AasI	GACNNNNNNGTC	2 cut(s)	770, 2334
Acc36I	ACCTGC	1 cut(s)	2669
AccBSI	CCGCTC	1 cut(s)	269
AccI	GTMKAC	1 cut(s)	1149
AccII	CGCG	1 cut(s)	324
AciI	CCGC	7 cut(s)	120, 132, 269, 322, 1530, 1558, 1614
AclI	AACGTT	1 cut(s)	2597
AclWI	GGATC	3 cut(s)	737, 891, 2308
AcoI	YGGCCR	1 cut(s)	1559
AcsI	RAATTY	3 cut(s)	543, 989, 1720
AcuI	CTGAAG	6 cut(s)	384, 641, 876, 1445, 2454, 2692
AcyI	GRCGYC	2 cut(s)	157, 1690
AfaI	GTAC	6 cut(s)	837, 1140, 1191, 2065, 2522, 2823
AfiI	CCNNNNNNNGG	2 cut(s)	698, 1445
AflIII	ACRYGT	1 cut(s)	945
AgsI	TTSAA	7 cut(s)	256, 439, 1751, 2074, 2153, 2464, 2836
AjnI	CCWGG	7 cut(s)	181, 547, 937, 1027, 1126, 1444, 2503
AjuI	GAANNNNNNNTTGG	2 cut(s)	1038, 1070
AleI	CACNNNNGTG	1 cut(s)	1256
AlwI	GGATC	3 cut(s)	737, 891, 2308
AlwNI	CAGNNNCTG	1 cut(s)	533
AoxI	GGCC	6 cut(s)	689, 1115, 1225, 1559, 2537, 2622
ApaI	GGGCCC	2 cut(s)	693, 1119
ApeKI	GCWGC	5 cut(s)	662, 1403, 1610, 1664, 2891
ApoI	RAATTY	3 cut(s)	543, 989, 1720
Asp700I	GAANNNNTTC	2 cut(s)	647, 1837
AspA2I	CCTAGG	1 cut(s)	1933
AspLEI	GCGC	1 cut(s)	326
AsuC2I	CCSGG	2 cut(s)	2253, 2546
AsuHPI	GGTGA	7 cut(s)	54, 109, 590, 953, 1078, 2018, 2540
AvaII	GGWCC	5 cut(s)	185, 608, 713, 1130, 2108
AvrII	CCTAGG	1 cut(s)	1933
BaeGI	GKGCMC	2 cut(s)	693, 1119
BaeI	ACNNNNGTAYC	2 cut(s)	2492, 2525
BanII	GRGCYC	2 cut(s)	693, 1119
BarI	GAAGNNNNNNTAC	6 cut(s)	910, 942, 1061, 1093, 2504, 2536
BauI	CACGAG	2 cut(s)	516, 2565
BbsI	GAAGAC	1 cut(s)	1982
BbvI	GCAGC	5 cut(s)	649, 1415, 1597, 1651, 2878
BccI	CCATC	5 cut(s)	619, 848, 1858, 2342, 2600
BceAI	ACGGC	1 cut(s)	144
BciT130I	CCWGG	7 cut(s)	183, 549, 939, 1029, 1128, 1446, 2505
BciVI	GTATCC	2 cut(s)	373, 382
BclI	TGATCA	1 cut(s)	2023
BcnI	CCSGG	2 cut(s)	2253, 2546
BfaI	CTAG	8 cut(s)	365, 678, 855, 1500, 1782, 1829, 1934, 2535
BfmI	CTRYAG	2 cut(s)	172, 2676
BfuAI	ACCTGC	1 cut(s)	2669
BfuI	GTATCC	2 cut(s)	373, 382
BglII	AGATCT	1 cut(s)	616
BisI	GCNGC	7 cut(s)	663, 1404, 1559, 1611, 1614, 1665, 2892
BlnI	CCTAGG	1 cut(s)	1933
BlpI	GCTNAGC	2 cut(s)	1477, 2390
BlsI	GCNGC	7 cut(s)	664, 1405, 1560, 1612, 1615, 1666, 2893
BmcAI	AGTACT	1 cut(s)	2823
Bme1390I	CCNGG	9 cut(s)	183, 549, 939, 1029, 1128, 1446, 2253, 2505, 2546
Bme18I	GGWCC	5 cut(s)	185, 608, 713, 1130, 2108
BmiI	GGNNCC	7 cut(s)	187, 609, 691, 714, 1116, 1117, 1930
BmrFI	CCNGG	9 cut(s)	183, 549, 939, 1029, 1128, 1446, 2253, 2505, 2546
BmrI	ACTGGG	1 cut(s)	2726
BmsI	GCATC	7 cut(s)	625, 1286, 1651, 2125, 2570, 2700, 2878
BmuI	ACTGGG	1 cut(s)	2726
BoxI	GACNNNNGTC	1 cut(s)	1030
BpiI	GAAGAC	1 cut(s)	1982
BpmI	CTGGAG	4 cut(s)	165, 293, 465, 1428
Bpu10I	CCTNAGC	1 cut(s)	1242
Bpu1102I	GCTNAGC	2 cut(s)	1477, 2390
BpuEI	CTTGAG	1 cut(s)	1529
BpuMI	CCSGG	2 cut(s)	2253, 2546
BsaHI	GRCGYC	2 cut(s)	157, 1690
BsaI	GGTCTC	4 cut(s)	183, 822, 1461, 2320
BsaJI	CCNNGG	6 cut(s)	666, 1028, 1757, 1933, 2504, 2649
BsaXI	ACNNNNNCTCC	2 cut(s)	1877, 1907
Bsc4I	CCNNNNNNNGG	2 cut(s)	698, 1445
Bse118I	RCCGGY	1 cut(s)	536
Bse1I	ACTGG	9 cut(s)	358, 376, 934, 1681, 1840, 2339, 2409, 2732, 2807
Bse3DI	GCAATG	3 cut(s)	231, 1870, 2766
BseBI	CCWGG	7 cut(s)	183, 549, 939, 1029, 1128, 1446, 2505
BseDI	CCNNGG	6 cut(s)	666, 1028, 1757, 1933, 2504, 2649
BseGI	GGATG	7 cut(s)	1096, 1317, 1813, 2140, 2611, 2623, 2694
BseLI	CCNNNNNNNGG	2 cut(s)	698, 1445
BseMI	GCAATG	3 cut(s)	231, 1870, 2766
BseMII	CTCAG	8 cut(s)	220, 540, 746, 833, 1213, 1224, 1236, 1859
BseNI	ACTGG	9 cut(s)	358, 376, 934, 1681, 1840, 2339, 2409, 2732, 2807
BseRI	GAGGAG	8 cut(s)	14, 39, 104, 127, 130, 1133, 1532, 2236
BseSI	GKGCMC	2 cut(s)	693, 1119
BseXI	GCAGC	5 cut(s)	649, 1415, 1597, 1651, 2878
BsgI	GTGCAG	1 cut(s)	451
Bsh1236I	CGCG	1 cut(s)	324
BshFI	GGCC	6 cut(s)	691, 1117, 1227, 1561, 2539, 2624
BsiSI	CCGG	3 cut(s)	537, 2253, 2546
BslFI	GGGAC	3 cut(s)	29, 147, 382
BslI	CCNNNNNNNGG	2 cut(s)	698, 1445
BsmBI	CGTCTC	1 cut(s)	26
BsmFI	GGGAC	3 cut(s)	29, 147, 382
BsmI	GAATGC	1 cut(s)	1243
BsnI	GGCC	6 cut(s)	691, 1117, 1227, 1561, 2539, 2624
Bso31I	GGTCTC	4 cut(s)	183, 822, 1461, 2320
Bsp120I	GGGCCC	2 cut(s)	689, 1115
Bsp1286I	GDGCHC	2 cut(s)	693, 1119
Bsp143I	GATC	7 cut(s)	499, 616, 742, 883, 1582, 2023, 2300
Bsp1720I	GCTNAGC	2 cut(s)	1477, 2390
Bsp19I	CCATGG	2 cut(s)	666, 1757
BspACI	CCGC	7 cut(s)	120, 132, 269, 322, 1530, 1558, 1614
BspANI	GGCC	6 cut(s)	691, 1117, 1227, 1561, 2539, 2624
BspCNI	CTCAG	8 cut(s)	219, 539, 745, 832, 1212, 1225, 1235, 1858
BspFNI	CGCG	1 cut(s)	324
BspHI	TCATGA	2 cut(s)	1627, 2086
BspLI	GGNNCC	7 cut(s)	187, 609, 691, 714, 1116, 1117, 1930
BspMAI	CTGCAG	1 cut(s)	2680
BspMI	ACCTGC	1 cut(s)	2669
BspPI	GGATC	3 cut(s)	737, 891, 2308
BspQI	GCTCTTC	2 cut(s)	259, 1746
BspTNI	GGTCTC	4 cut(s)	183, 822, 1461, 2320
BsrBI	CCGCTC	1 cut(s)	269
BsrDI	GCAATG	3 cut(s)	231, 1870, 2766
BsrFI	RCCGGY	1 cut(s)	536
BsrI	ACTGG	9 cut(s)	358, 376, 934, 1681, 1840, 2339, 2409, 2732, 2807
BssAI	RCCGGY	1 cut(s)	536
BssECI	CCNNGG	6 cut(s)	666, 1028, 1757, 1933, 2504, 2649
BssMI	GATC	7 cut(s)	499, 616, 742, 883, 1582, 2023, 2300
BssNI	GRCGYC	2 cut(s)	157, 1690
BssSI	CACGAG	2 cut(s)	516, 2565
BssT1I	CCWWGG	4 cut(s)	666, 1757, 1933, 2649
Bst2BI	CACGAG	2 cut(s)	516, 2565
Bst2UI	CCWGG	7 cut(s)	183, 549, 939, 1029, 1128, 1446, 2505
Bst4CI	ACNGT	2 cut(s)	1431, 1741
Bst6I	CTCTTC	6 cut(s)	92, 259, 1464, 1707, 1746, 2318
BstACI	GRCGYC	2 cut(s)	157, 1690
BstAPI	GCANNNNNTGC	3 cut(s)	1400, 2386, 2900
BstC8I	GCNNGC	3 cut(s)	203, 571, 2714
BstDSI	CCRYGG	2 cut(s)	666, 1757
BstF5I	GGATG	7 cut(s)	1096, 1317, 1813, 2140, 2611, 2623, 2694
BstFNI	CGCG	1 cut(s)	324
BstHHI	GCGC	1 cut(s)	326
BstKTI	GATC	7 cut(s)	502, 619, 745, 886, 1585, 2026, 2303
BstMBI	GATC	7 cut(s)	499, 616, 742, 883, 1582, 2023, 2300
BstMWI	GCNNNNNNNGC	4 cut(s)	1400, 1976, 2386, 2900
BstNI	CCWGG	7 cut(s)	183, 549, 939, 1029, 1128, 1446, 2505
BstNSI	RCATGY	3 cut(s)	949, 2755, 2766
BstPAI	GACNNNNGTC	1 cut(s)	1030
BstSCI	CCNGG	9 cut(s)	181, 547, 937, 1027, 1126, 1444, 2251, 2503, 2544
BstSFI	CTRYAG	2 cut(s)	172, 2676
BstSLI	GKGCMC	2 cut(s)	693, 1119
BstUI	CGCG	1 cut(s)	324
BstV1I	GCAGC	5 cut(s)	649, 1415, 1597, 1651, 2878
BstV2I	GAAGAC	1 cut(s)	1982
BstX2I	RGATCY	3 cut(s)	616, 742, 2300
BstXI	CCANNNNNNTGG	1 cut(s)	1452
BstYI	RGATCY	3 cut(s)	616, 742, 2300
BsuI	GTATCC	2 cut(s)	373, 382
BsuRI	GGCC	6 cut(s)	691, 1117, 1227, 1561, 2539, 2624
BtgI	CCRYGG	2 cut(s)	666, 1757
BtsCI	GGATG	7 cut(s)	1096, 1317, 1813, 2140, 2611, 2623, 2694
BtsI	GCAGTG	2 cut(s)	1491, 2434
BtsIMutI	CAGTG	4 cut(s)	408, 1491, 1876, 2434
BveI	ACCTGC	1 cut(s)	2669
Cac8I	GCNNGC	3 cut(s)	203, 571, 2714
CaiI	CAGNNNCTG	1 cut(s)	533
CciI	TCATGA	2 cut(s)	1627, 2086
CfoI	GCGC	1 cut(s)	326
Cfr10I	RCCGGY	1 cut(s)	536
CseI	GACGC	3 cut(s)	165, 1679, 2187
Csp6I	GTAC	6 cut(s)	836, 1139, 1190, 2064, 2521, 2822
CspCI	CAANNNNNGTGG	2 cut(s)	1587, 1622
CviQI	GTAC	6 cut(s)	836, 1139, 1190, 2064, 2521, 2822
DpnI	GATC	7 cut(s)	501, 618, 744, 885, 1584, 2025, 2302
DpnII	GATC	7 cut(s)	499, 616, 742, 883, 1582, 2023, 2300
DraI	TTTAAA	2 cut(s)	2205, 2848
DrdI	GACNNNNNNGTC	2 cut(s)	770, 2334
DseDI	GACNNNNNNGTC	2 cut(s)	770, 2334
EaeI	YGGCCR	1 cut(s)	1559
Eam1104I	CTCTTC	6 cut(s)	92, 259, 1464, 1707, 1746, 2318
EarI	CTCTTC	6 cut(s)	92, 259, 1464, 1707, 1746, 2318
EciI	GGCGGA	1 cut(s)	121
Eco130I	CCWWGG	4 cut(s)	666, 1757, 1933, 2649
Eco24I	GRGCYC	2 cut(s)	693, 1119
Eco31I	GGTCTC	4 cut(s)	183, 822, 1461, 2320
Eco47I	GGWCC	5 cut(s)	185, 608, 713, 1130, 2108
Eco57I	CTGAAG	6 cut(s)	384, 641, 876, 1445, 2454, 2692
EcoO109I	RGGNCCY	5 cut(s)	185, 713, 1115, 1116, 2538
EcoRI	GAATTC	1 cut(s)	543
EcoRII	CCWGG	7 cut(s)	181, 547, 937, 1027, 1126, 1444, 2503
EcoT14I	CCWWGG	4 cut(s)	666, 1757, 1933, 2649
EcoT22I	ATGCAT	3 cut(s)	2140, 2757, 2768
EcoT38I	GRGCYC	2 cut(s)	693, 1119
ErhI	CCWWGG	4 cut(s)	666, 1757, 1933, 2649
Esp3I	CGTCTC	1 cut(s)	26
FalI	AAGNNNNNCTT	1 cut(s)	2887
FaqI	GGGAC	3 cut(s)	29, 147, 382
FauNDI	CATATG	1 cut(s)	1564
FbaI	TGATCA	1 cut(s)	2023
FblI	GTMKAC	1 cut(s)	1149
Fnu4HI	GCNGC	7 cut(s)	663, 1404, 1559, 1611, 1614, 1665, 2892
FokI	GGATG	7 cut(s)	1083, 1324, 1820, 2147, 2618, 2630, 2681
FriOI	GRGCYC	2 cut(s)	693, 1119
Fsp4HI	GCNGC	7 cut(s)	663, 1404, 1559, 1611, 1614, 1665, 2892
FspBI	CTAG	8 cut(s)	365, 678, 855, 1500, 1782, 1829, 1934, 2535
GlaI	GCGC	1 cut(s)	325
GluI	GCNGC	7 cut(s)	663, 1404, 1559, 1611, 1614, 1665, 2892
GsuI	CTGGAG	4 cut(s)	165, 293, 465, 1428
HaeIII	GGCC	6 cut(s)	691, 1117, 1227, 1561, 2539, 2624
HapII	CCGG	3 cut(s)	537, 2253, 2546
HgaI	GACGC	3 cut(s)	165, 1679, 2187
HhaI	GCGC	1 cut(s)	326
Hin1I	GRCGYC	2 cut(s)	157, 1690
Hin6I	GCGC	1 cut(s)	324
HinP1I	GCGC	1 cut(s)	324
HincII	GTYRAC	4 cut(s)	561, 760, 1150, 2344
HindII	GTYRAC	4 cut(s)	561, 760, 1150, 2344
HindIII	AAGCTT	1 cut(s)	2513
HpaII	CCGG	3 cut(s)	537, 2253, 2546
HphI	GGTGA	7 cut(s)	54, 109, 590, 953, 1078, 2018, 2540
Hpy188I	TCNGA	9 cut(s)	209, 529, 616, 621, 895, 1075, 1642, 2473, 2875
Hpy99I	CGWCG	4 cut(s)	159, 767, 1151, 2064
HpyAV	CCTTC	8 cut(s)	26, 49, 408, 578, 806, 1919, 2068, 2458
HpyCH4III	ACNGT	2 cut(s)	1431, 1741
HpyCH4IV	ACGT	3 cut(s)	2062, 2239, 2597
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1400, 1976, 2386, 2900
HpySE526I	ACGT	3 cut(s)	2062, 2239, 2597
Hsp92I	GRCGYC	2 cut(s)	157, 1690
HspAI	GCGC	1 cut(s)	324
Ksp22I	TGATCA	1 cut(s)	2023
Kzo9I	GATC	7 cut(s)	499, 616, 742, 883, 1582, 2023, 2300
LguI	GCTCTTC	2 cut(s)	259, 1746
LmnI	GCTCC	3 cut(s)	681, 828, 1447
Lsp1109I	GCAGC	5 cut(s)	649, 1415, 1597, 1651, 2878
LweI	GCATC	7 cut(s)	625, 1286, 1651, 2125, 2570, 2700, 2878
MaeI	CTAG	8 cut(s)	365, 678, 855, 1500, 1782, 1829, 1934, 2535
MaeII	ACGT	3 cut(s)	2062, 2239, 2597
MaeIII	GTNAC	4 cut(s)	275, 941, 1249, 2240
MalI	GATC	7 cut(s)	501, 618, 744, 885, 1584, 2025, 2302
MbiI	CCGCTC	1 cut(s)	269
MboI	GATC	7 cut(s)	499, 616, 742, 883, 1582, 2023, 2300
MflI	RGATCY	3 cut(s)	616, 742, 2300
MhlI	GDGCHC	2 cut(s)	693, 1119
MlyI	GAGTC	4 cut(s)	251, 1279, 1404, 1496
MmeI	TCCRAC	2 cut(s)	2086, 2470
Mph1103I	ATGCAT	3 cut(s)	2140, 2757, 2768
MroXI	GAANNNNTTC	2 cut(s)	647, 1837
MseI	TTAA	8 cut(s)	69, 483, 597, 1283, 2204, 2231, 2790, 2847
MslI	CAYNNNNRTG	3 cut(s)	1101, 1256, 2016
MspA1I	CMGCKG	2 cut(s)	1532, 1942
MspI	CCGG	3 cut(s)	537, 2253, 2546
MspR9I	CCNGG	9 cut(s)	183, 549, 939, 1029, 1128, 1446, 2253, 2505, 2546
Mva1269I	GAATGC	1 cut(s)	1243
MvaI	CCWGG	7 cut(s)	183, 549, 939, 1029, 1128, 1446, 2505
MvnI	CGCG	1 cut(s)	324
MwoI	GCNNNNNNNGC	4 cut(s)	1400, 1976, 2386, 2900
NciI	CCSGG	2 cut(s)	2253, 2546
NcoI	CCATGG	2 cut(s)	666, 1757
NdeI	CATATG	1 cut(s)	1564
NdeII	GATC	7 cut(s)	499, 616, 742, 883, 1582, 2023, 2300
NlaIV	GGNNCC	7 cut(s)	187, 609, 691, 714, 1116, 1117, 1930
NmuCI	GTSAC	4 cut(s)	275, 941, 1249, 2240
NsiI	ATGCAT	3 cut(s)	2140, 2757, 2768
NspI	RCATGY	3 cut(s)	949, 2755, 2766
OliI	CACNNNNGTG	1 cut(s)	1256
PagI	TCATGA	2 cut(s)	1627, 2086
PaqCI	CACCTGC	1 cut(s)	2669
PciI	ACATGT	1 cut(s)	945
PciSI	GCTCTTC	2 cut(s)	259, 1746
PctI	GAATGC	1 cut(s)	1243
PdmI	GAANNNNTTC	2 cut(s)	647, 1837
PfeI	GAWTC	8 cut(s)	304, 850, 1163, 1261, 1372, 1645, 2279, 2870
PflFI	GACNNNGTC	2 cut(s)	159, 241
PfoI	TCCNGGA	2 cut(s)	181, 2251
PkrI	GCNGC	7 cut(s)	664, 1405, 1560, 1612, 1615, 1666, 2893
PleI	GAGTC	4 cut(s)	250, 1278, 1404, 1495
PpsI	GAGTC	4 cut(s)	250, 1278, 1404, 1495
PpuMI	RGGWCCY	2 cut(s)	185, 713
PscI	ACATGT	1 cut(s)	945
PshAI	GACNNNNGTC	1 cut(s)	1030
Psp1406I	AACGTT	1 cut(s)	2597
Psp5II	RGGWCCY	2 cut(s)	185, 713
Psp6I	CCWGG	7 cut(s)	181, 547, 937, 1027, 1126, 1444, 2503
PspGI	CCWGG	7 cut(s)	181, 547, 937, 1027, 1126, 1444, 2503
PspN4I	GGNNCC	7 cut(s)	187, 609, 691, 714, 1116, 1117, 1930
PspOMI	GGGCCC	2 cut(s)	689, 1115
PspPPI	RGGWCCY	2 cut(s)	185, 713
PstI	CTGCAG	1 cut(s)	2680
PstNI	CAGNNNCTG	1 cut(s)	533
PsuI	RGATCY	3 cut(s)	616, 742, 2300
PsyI	GACNNNGTC	2 cut(s)	159, 241
PvuII	CAGCTG	1 cut(s)	1942
RsaI	GTAC	6 cut(s)	837, 1140, 1191, 2065, 2522, 2823
RsaNI	GTAC	6 cut(s)	836, 1139, 1190, 2064, 2521, 2822
RseI	CAYNNNNRTG	3 cut(s)	1101, 1256, 2016
SalI	GTCGAC	1 cut(s)	1148
SapI	GCTCTTC	2 cut(s)	259, 1746
SaqAI	TTAA	8 cut(s)	69, 483, 597, 1283, 2204, 2231, 2790, 2847
SatI	GCNGC	7 cut(s)	663, 1404, 1559, 1611, 1614, 1665, 2892
Sau3AI	GATC	7 cut(s)	499, 616, 742, 883, 1582, 2023, 2300
ScaI	AGTACT	1 cut(s)	2823
SchI	GAGTC	4 cut(s)	251, 1279, 1404, 1496
ScrFI	CCNGG	9 cut(s)	183, 549, 939, 1029, 1128, 1446, 2253, 2505, 2546
SduI	GDGCHC	2 cut(s)	693, 1119
SfaNI	GCATC	7 cut(s)	625, 1286, 1651, 2125, 2570, 2700, 2878
SfcI	CTRYAG	2 cut(s)	172, 2676
SinI	GGWCC	5 cut(s)	185, 608, 713, 1130, 2108
SmiMI	CAYNNNNRTG	3 cut(s)	1101, 1256, 2016
SmlI	CTYRAG	1 cut(s)	1544
SmoI	CTYRAG	1 cut(s)	1544
SsiI	CCGC	7 cut(s)	120, 132, 269, 322, 1530, 1558, 1614
SspMI	CTAG	8 cut(s)	365, 678, 855, 1500, 1782, 1829, 1934, 2535
StyD4I	CCNGG	9 cut(s)	181, 547, 937, 1027, 1126, 1444, 2251, 2503, 2544
StyI	CCWWGG	4 cut(s)	666, 1757, 1933, 2649
TaaI	ACNGT	2 cut(s)	1431, 1741
TaiI	ACGT	3 cut(s)	2065, 2242, 2600
TaqI	TCGA	6 cut(s)	1149, 1408, 1993, 2601, 2865, 2886
TaqII	GACCGA	2 cut(s)	254, 1575
TatI	WGTACW	3 cut(s)	1138, 2520, 2821
TauI	GCSGC	2 cut(s)	1561, 1616
TfiI	GAWTC	8 cut(s)	304, 850, 1163, 1261, 1372, 1645, 2279, 2870
Tru1I	TTAA	8 cut(s)	69, 483, 597, 1283, 2204, 2231, 2790, 2847
Tru9I	TTAA	8 cut(s)	69, 483, 597, 1283, 2204, 2231, 2790, 2847
TscAI	CASTG	4 cut(s)	408, 1498, 1876, 2441
TseFI	GTSAC	4 cut(s)	275, 941, 1249, 2240
TseI	GCWGC	5 cut(s)	662, 1403, 1610, 1664, 2891
Tsp45I	GTSAC	4 cut(s)	275, 941, 1249, 2240
TspGWI	ACGGA	3 cut(s)	235, 940, 1177
TspRI	CASTG	4 cut(s)	408, 1498, 1876, 2441
Tth111I	GACNNNGTC	2 cut(s)	159, 241
VpaK11BI	GGWCC	5 cut(s)	185, 608, 713, 1130, 2108
XapI	RAATTY	3 cut(s)	543, 989, 1720
XceI	RCATGY	3 cut(s)	949, 2755, 2766
XmaJI	CCTAGG	1 cut(s)	1933
XmiI	GTMKAC	1 cut(s)	1149
XmnI	GAANNNNTTC	2 cut(s)	647, 1837
XspI	CTAG	8 cut(s)	365, 678, 855, 1500, 1782, 1829, 1934, 2535
ZrmI	AGTACT	1 cut(s)	2823
Zsp2I	ATGCAT	3 cut(s)	2140, 2757, 2768

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.