MD03G1198300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016214
Physical Location & Seq
Forward (+)
27135502 .. 27136273
772 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1198300.v1.1.491

Sequence Viewer

Length: 480 bp
ATGCAGGTGAAGACAATCAATTCAATTCTCTTTTATTTTTCCCAGCCAATAAACATTATCCAAAACAAAATCTCCCCAATTGGCCTGTTGAAACCACCTTTTCTGCTAAGTAGTTGGGATTTCACGATGAGGTGCATCCTGTTTCACCCAGCACTGCTTCCTCTGCACACCCAGCTAAACCAAAGACAGCAGCTGCTGGGTACGCATTCCACTAAACTTGCTGCTGCACCTGCACCACATAGGCAGCAGCACCTGCTCTCGTTAAGAACCAGGGCCATGCAAAACGCTGTTACTTTTCTCGGTGTCGGTCCCTCTGCCAAATCCGGTGGAGACATTTCTGTCCTCCTCCAAACTGGCGGGGTGCTTCTTTTGGTTTACTTGTTTTCCAACTTCGTTGTGCCGGTTCTCGTTTCGAAATTTTTCGGGTTCGATAAGGTGGGTGAAGAAGATGAAGACGATAACGACCTATTTAACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

17.73

Weight (kDa)

9.25

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50732
fragaria_vesca FvH4_3g23030
malus_domestica MD03G1198300.v1.1
prunus_persica Prupe.4G216700_v2.0.a1
pyrus_communis pycom03g14990
rosa_laevigata RLG00000033985
rosa_roxburghii Rroxscaffold_1G00040140 Rroxscaffold_1G00040870
rosa_rugosa Rorug05G0185400 Rorug05G0185500
rosa_samantha Rh5AG272300 Rh5BG276300 Rh5CG309000 Rh5DG284800
rosa_wichuraiana Rw5G025500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 238, 261
AasI GACNNNNNNGTC 1 cut(s) 338
Acc36I ACCTGC 2 cut(s) 238, 261
AciI CCGC 1 cut(s) 357
AcsI RAATTY 1 cut(s) 416
AfaI GTAC 1 cut(s) 202
AgsI TTSAA 2 cut(s) 24, 91
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 2 cut(s) 175, 193
AluI AGCT 2 cut(s) 175, 193
Alw26I GTCTC 1 cut(s) 324
AlwNI CAGNNNCTG 3 cut(s) 193, 196, 253
AoxI GGCC 2 cut(s) 82, 273
ApeKI GCWGC 6 cut(s) 190, 193, 221, 224, 244, 247
ApoI RAATTY 1 cut(s) 416
Asp700I GAANNNNTTC 1 cut(s) 419
AspS9I GGNCC 2 cut(s) 273, 308
AsuHPI GGTGA 3 cut(s) 19, 137, 452
AsuII TTCGAA 1 cut(s) 413
AvaII GGWCC 1 cut(s) 308
BbsI GAAGAC 2 cut(s) 17, 459
BbvI GCAGC 6 cut(s) 180, 202, 208, 211, 256, 259
BciT130I CCWGG 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 324
BfuAI ACCTGC 2 cut(s) 238, 261
BisI GCNGC 6 cut(s) 191, 194, 222, 225, 245, 248
BlsI GCNGC 6 cut(s) 192, 195, 223, 226, 246, 249
Bme1390I CCNGG 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 273, 308
BmiI GGNNCC 1 cut(s) 310
BmrFI CCNGG 1 cut(s) 271
BmsI GCATC 1 cut(s) 144
BpiI GAAGAC 2 cut(s) 17, 459
Bpu14I TTCGAA 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 323
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bse118I RCCGGY 1 cut(s) 400
Bse1I ACTGG 1 cut(s) 358
BseBI CCWGG 1 cut(s) 271
BseDI CCNNGG 1 cut(s) 270
BseGI GGATG 1 cut(s) 135
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 1 cut(s) 335
BseXI GCAGC 6 cut(s) 180, 202, 208, 211, 256, 259
BseYI CCCAGC 4 cut(s) 42, 148, 171, 196
BsgI GTGCAG 3 cut(s) 149, 210, 216
BshFI GGCC 2 cut(s) 84, 275
BsiSI CCGG 2 cut(s) 324, 401
BslFI GGGAC 1 cut(s) 294
BsmAI GTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 294
BsmI GAATGC 1 cut(s) 205
BsnI GGCC 2 cut(s) 84, 275
Bsp119I TTCGAA 1 cut(s) 413
BspACI CCGC 1 cut(s) 357
BspANI GGCC 2 cut(s) 84, 275
BspLI GGNNCC 1 cut(s) 310
BspMI ACCTGC 2 cut(s) 238, 261
BspT104I TTCGAA 1 cut(s) 413
BsrFI RCCGGY 1 cut(s) 400
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 1 cut(s) 400
BssECI CCNNGG 1 cut(s) 270
Bst2UI CCWGG 1 cut(s) 271
BstAPI GCANNNNNTGC 1 cut(s) 253
BstBI TTCGAA 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 107
BstF5I GGATG 1 cut(s) 135
BstMAI GTCTC 1 cut(s) 324
BstMWI GCNNNNNNNGC 5 cut(s) 163, 172, 202, 230, 253
BstNI CCWGG 1 cut(s) 271
BstSCI CCNGG 1 cut(s) 269
BstV1I GCAGC 6 cut(s) 180, 202, 208, 211, 256, 259
BstV2I GAAGAC 2 cut(s) 17, 459
BsuRI GGCC 2 cut(s) 84, 275
BtsCI GGATG 1 cut(s) 135
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 1 cut(s) 152
BveI ACCTGC 2 cut(s) 238, 261
CaiI CAGNNNCTG 3 cut(s) 193, 196, 253
Cfr10I RCCGGY 1 cut(s) 400
Cfr13I GGNCC 2 cut(s) 273, 308
Csp6I GTAC 1 cut(s) 201
CspCI CAANNNNNGTGG 4 cut(s) 199, 234, 307, 342
CviAII CATG 1 cut(s) 277
CviJI RGCY 5 cut(s) 46, 84, 175, 193, 275
CviKI_1 RGCY 5 cut(s) 46, 84, 175, 193, 275
CviQI GTAC 1 cut(s) 201
DdeI CTNAG 1 cut(s) 107
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
Eco47I GGWCC 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 269
FaeI CATG 1 cut(s) 280
FaiI YATR 2 cut(s) 240, 278
FaqI GGGAC 1 cut(s) 294
FatI CATG 1 cut(s) 276
FauI CCCGC 1 cut(s) 350
Fnu4HI GCNGC 6 cut(s) 191, 194, 222, 225, 245, 248
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 6 cut(s) 191, 194, 222, 225, 245, 248
GluI GCNGC 6 cut(s) 191, 194, 222, 225, 245, 248
GsaI CCCAGC 4 cut(s) 46, 152, 175, 200
HaeIII GGCC 2 cut(s) 84, 275
HapII CCGG 2 cut(s) 324, 401
Hin1II CATG 1 cut(s) 280
HpaII CCGG 2 cut(s) 324, 401
HphI GGTGA 3 cut(s) 19, 137, 452
Hpy166II GTNNAC 1 cut(s) 376
Hpy188III TCNNGA 1 cut(s) 124
Hpy8I GTNNAC 1 cut(s) 376
HpyCH4V TGCA 6 cut(s) 4, 135, 166, 227, 233, 280
HpyF10VI GCNNNNNNNGC 5 cut(s) 163, 172, 202, 230, 253
HpyF3I CTNAG 1 cut(s) 107
Hsp92II CATG 1 cut(s) 280
Lsp1109I GCAGC 6 cut(s) 180, 202, 208, 211, 256, 259
LweI GCATC 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 289
MboII GAAGA 4 cut(s) 22, 455, 458, 464
MfeI CAATTG 1 cut(s) 78
MluCI AATT 4 cut(s) 19, 24, 78, 416
MmeI TCCRAC 1 cut(s) 411
MnlI CCTC 5 cut(s) 123, 171, 322, 353, 356
MroXI GAANNNNTTC 1 cut(s) 419
MseI TTAA 2 cut(s) 263, 471
MspA1I CMGCKG 1 cut(s) 193
MspI CCGG 2 cut(s) 324, 401
MspR9I CCNGG 1 cut(s) 271
MunI CAATTG 1 cut(s) 78
Mva1269I GAATGC 1 cut(s) 205
MvaI CCWGG 1 cut(s) 271
MwoI GCNNNNNNNGC 5 cut(s) 163, 172, 202, 230, 253
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 310
NspV TTCGAA 1 cut(s) 413
PaqCI CACCTGC 2 cut(s) 238, 261
PctI GAATGC 1 cut(s) 205
PdmI GAANNNNTTC 1 cut(s) 419
PkrI GCNGC 6 cut(s) 192, 195, 223, 226, 246, 249
Psp6I CCWGG 1 cut(s) 269
PspFI CCCAGC 4 cut(s) 42, 148, 171, 196
PspGI CCWGG 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 310
PspPI GGNCC 2 cut(s) 273, 308
PstNI CAGNNNCTG 3 cut(s) 193, 196, 253
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 1 cut(s) 202
RsaNI GTAC 1 cut(s) 201
SaqAI TTAA 2 cut(s) 263, 471
SatI GCNGC 6 cut(s) 191, 194, 222, 225, 245, 248
Sau96I GGNCC 2 cut(s) 273, 308
ScrFI CCNGG 1 cut(s) 271
SfaNI GCATC 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 1 cut(s) 308
Sse9I AATT 4 cut(s) 19, 24, 78, 416
SsiI CCGC 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 269
TaqI TCGA 2 cut(s) 413, 429
TaqII GACCGA 1 cut(s) 296
TasI AATT 4 cut(s) 19, 24, 78, 416
Tru1I TTAA 2 cut(s) 263, 471
Tru9I TTAA 2 cut(s) 263, 471
TscAI CASTG 1 cut(s) 159
TseI GCWGC 6 cut(s) 190, 193, 221, 224, 244, 247
TspDTI ATGAA 1 cut(s) 465
TspRI CASTG 1 cut(s) 159
VpaK11BI GGWCC 1 cut(s) 308
XapI RAATTY 1 cut(s) 416
XmnI GAANNNNTTC 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.