Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005489

Physical Location & Seq

Genomic Coordinates Reverse (-)

27600259 .. 27605867

Gene Structure 5609 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1201700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2259 bp

ATGAGTGTGGTGGGGTTTGATGTTGGAAATGAGAATTGTGTAATTGCGGTGGTGAAGCAACGGGGAGTTGATGTTTTGTTGAATGATGAATCGAAACGTGAAACCCCAGCTGTGGTTTGTTTTGGTGAGAAGCAGAGGTTTCTGGGGTCTGCTGGTGCTGCCTCTGCAATGATGAACCCGAAATCTACGGTGTCTCAGGTGAAGAGACTGATCGGTAGAAAATTTACTGAGCCTGATGTTCAACGTGACCTTCGAATTTTGCCCTTTGAAACTTCTGAAGCTCCAGATGGTGGCATTTTGATTCATTTGAAGTACTTGGGCGCGACTCATACATTTACGCCAGTTCAAGTTACAGCAATGCTTTTTGCACATTTGAAAGATCTAATAGAGAAGAATCAGGAAATGCCCATTTCAGATTGTGTGATTGGAATTCCATCGTACTTTACAGACTTCCAAAGACGTGCATATTTGGATGCTGCCACAGTTGCTGGGTTGAAGCCTTTGAGATTGATGCATGACTGTACTGCAACTGCTCATAGTTATGGAGTTTACAAAACAGATTTCTCAACTTCTGGTCCAACTTATGTGGCATTTATTGACATTGGTCACTGTGATACACAGGTCTCTATTGCGTCATTTGAGGCTTGGCAAATGAAGATACTATCTCATACTTTTGACAGAAGCTTGGGAGGGAGAGACTTCGATGAGGTCTTGTTTAATCATTTTGCCACACAGTTCAAGGAGCAGTACAGGATTGATGTGTACTCTAATGTGAAGGCAAGTATCAGGCTACGGGCAGCATGTGAGAAGCTGAAGAAAGTGTTGAGTGCGAATACGGAGGCACCTCTTAATATTGAGTGCTTAATGGATGAAAAAGATGTCAGGGGATTTATTAAAAGGGAAGACTTTGAGATGCTGGCTTCAGGTTTGTTGGAGAGAATTGGTGTTCCTTGTAGCAAAGCTCTATCTGATGCAGGTTTGACTGCTGATAAGATTCATTCTGTGGAGCTTGTCGGGTCAGGGTCTAGGATTCCAGCGGTTGCTAGAATATTAGCTTCTGTGTTCAGGAAAGAACCTGGCCGGACGCTGAATGCGAGTGAGTGTGTAGCACGTGGATGCGCTCTTCAATGTGCAATGCTCAGTCCTGTTTTTCGAGTCAGAGAACATGAGGTTCAAGACTCAATTCCTTTCTCAATAGCATTCTTAATCGATGAAGCTCCAATTTGCACAGGGACAAGTGGCATCCTGTTTCCAAAAGGCCAACCCATTCCAAGTGTTAAAGAACTGATGTTTCGACGAAGCAGTTTATTTCATTTGAAAGCATTCTATGCTAATCCCAGCGAAGTGCCTGCTGGCACTTCTCCTGACATTTGTTGTTTTACTATTGGCCCTTTCCAATGCTCCCATGGCGAAAAAACAAGGGTTAAAGTTAAAGTTATGTTAGATCTCCACGGTGTTGTCTCTGTTGAATCAGCTATGGTGATGGAAGATCACGGAGATGACTCTTCTACAGGGGGTCTTGCCGGTTCAAAAATGGAACCAATGGACACTGATTATGTAACTGCTTCTGGTTCTACTGAGGCAGTTGCAGATGGATTTGAAGAAAGCAGTATGCAGCACAACTCTTCACATACTGGTGGTGAGGCTAAAAGAAACAAAAAAGCCACCAGGACGCTAGAGATACCTGTTAGTGAAAGTATATATGGTGGAATGACCAAGGCTGAACTTTCAGAAGCTCAAGAAAAAGAACTCCAGTTAGCTCAACAGGACAAAATTATGGAACAAACCAAAGACAAAAAGAATGCGTTGGAGTCTTACGTCTATGAGATGCAAAATAAGCTTCTCAACACATATCGAAGCTTTGCAAGTTATCAAGAGATGGAGGGCATCTCCAGGAGCCTGCAGCAGACAGAGGAATGGCTTTATGATGATGGAGAAGATGAAACAGAAAATGCTTATACTTCAAGGCTGGAAGATCTTCAGAAGCTGGTAAATCCAATTGAGAACCGATATAAAGACAAGGAAGCAAGATTGCAAGCTACAAGAGATCTATTAAAGTGCATTGGGGATTACCGGATGGCTGCGAATTCAGTTTTTAATGAGTGCTATAAAGTGGAGCGGTGGCTTAGGGAGAAAAACCAACAACAAGACTCGTTGCCAAAGAATGTTGACCCAGTCTTATGGTCAAATGATATTAAGAGCAGGAATGAGGAATTGAAGTCGAGATGCAACCATGTATTCAGATCCAGGGCTCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04530 ko04612 map04530 map04612

Pfam Domains

Protein Families

HSP70

Protein Analysis

753

Amino Acids

83.9

Weight (kDa)

5.83

Isoelectric Point (pI)

43.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	3 - 671	4e-139	Hsp70 protein
MreB_Mbl	PF06723	116 - 375	2.6e-06	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005489)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11660 AT1G11660 AT1G11660 AT1G11660
fragaria_vesca	FvH4_3g22620 FvH4_3g22620 FvH4_3g22620 FvH4_3g22620 FvH4_3g22620
malus_domestica	MD03G1201700.v1.1 MD03G1201800.v1.1 MD11G1218900.v1.1
prunus_persica	Prupe.4G212200_v2.0.a1
pyrus_communis	pycom03g15300 pycom03g15320 pycom11g19290
rosa_chinensis	RchiOBHm_Chr5g0039371
rosa_laevigata	RLG00000033908
rosa_multiflora	Rmu_co8453851.1_g000001 Rmu_sc0006937.1_g000008 Rmu_sc0007310.1_g000013
rosa_roxburghii	Rroxscaffold_1G00040520 Rroxscaffold_1G00041830
rosa_rugosa	Rorug05G0178400
rosa_samantha	Rh5AG264600 Rh5BG269600 Rh5CG302600 Rh5DG277400
rosa_wichuraiana	Rw5G024820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	965
AccB1I	GGYRCC	1 cut(s)	841
AccB7I	CCANNNNNTGG	1 cut(s)	290
AccBSI	CCGCTC	1 cut(s)	2119
AccII	CGCG	1 cut(s)	323
AciI	CCGC	3 cut(s)	47, 1037, 2119
AclWI	GGATC	1 cut(s)	2238
AcoI	YGGCCR	1 cut(s)	1078
AcsI	RAATTY	4 cut(s)	221, 255, 429, 2086
AcuI	CTGAAG	4 cut(s)	297, 833, 906, 1964
AcvI	CACGTG	1 cut(s)	1112
AfaI	GTAC	5 cut(s)	314, 440, 523, 749, 764
AfiI	CCNNNNNNNGG	2 cut(s)	112, 290
AjiI	CACGTC	1 cut(s)	461
AjnI	CCWGG	4 cut(s)	1075, 1667, 1892, 2246
Alw26I	GTCTC	5 cut(s)	198, 199, 628, 690, 1465
AlwI	GGATC	1 cut(s)	2238
AlwNI	CAGNNNCTG	2 cut(s)	488, 1987
AoxI	GGCC	3 cut(s)	1078, 1258, 1387
ApeKI	GCWGC	6 cut(s)	158, 476, 797, 1615, 1903, 2081
ApoI	RAATTY	4 cut(s)	221, 255, 429, 2086
Asp700I	GAANNNNTTC	2 cut(s)	1322, 1977
AspLEI	GCGC	2 cut(s)	323, 1121
AspS9I	GGNCC	2 cut(s)	575, 1388
AsuHPI	GGTGA	5 cut(s)	64, 137, 211, 1492, 1652
AsuII	TTCGAA	1 cut(s)	253
AvaII	GGWCC	1 cut(s)	575
BaeI	ACNNNNGTAYC	2 cut(s)	606, 639
BanI	GGYRCC	1 cut(s)	841
BanII	GRGCYC	1 cut(s)	2254
BbrPI	CACGTG	1 cut(s)	1112
BbsI	GAAGAC	1 cut(s)	909
BbvI	GCAGC	6 cut(s)	145, 463, 809, 1627, 1915, 2068
BccI	CCATC	7 cut(s)	281, 442, 1477, 1586, 1873, 1925, 2071
BcgI	CGANNNNNNTGC	2 cut(s)	1331, 1365
BciT130I	CCWGG	4 cut(s)	1077, 1669, 1894, 2248
BcoDI	GTCTC	5 cut(s)	198, 199, 628, 690, 1465
BfaI	CTAG	3 cut(s)	1026, 1044, 1676
BfmI	CTRYAG	2 cut(s)	1509, 1901
BfuAI	ACCTGC	1 cut(s)	965
BglII	AGATCT	4 cut(s)	379, 1444, 1975, 2047
BisI	GCNGC	6 cut(s)	159, 477, 798, 1616, 1904, 2082
BlsI	GCNGC	6 cut(s)	160, 478, 799, 1617, 1905, 2083
BmcAI	AGTACT	1 cut(s)	314
Bme1390I	CCNGG	4 cut(s)	1077, 1669, 1894, 2248
Bme18I	GGWCC	1 cut(s)	575
BmgBI	CACGTC	1 cut(s)	461
BmgT120I	GGNCC	2 cut(s)	575, 1388
BmiI	GGNNCC	3 cut(s)	843, 1539, 1898
BmrFI	CCNGG	4 cut(s)	1077, 1669, 1894, 2248
BmrI	ACTGGG	1 cut(s)	2168
BmsI	GCATC	9 cut(s)	463, 501, 903, 961, 1106, 1251, 1818, 1896, 2216
BmuI	ACTGGG	1 cut(s)	2168
BoxI	GACNNNNGTC	1 cut(s)	603
BpiI	GAAGAC	1 cut(s)	909
BplI	GAGNNNNNCTC	2 cut(s)	1874, 1906
BpmI	CTGGAG	3 cut(s)	267, 1736, 1876
Bpu10I	CCTNAGC	1 cut(s)	2126
Bpu14I	TTCGAA	1 cut(s)	253
BpuEI	CTTGAG	1 cut(s)	1722
Bsa29I	ATCGAT	1 cut(s)	1209
BsaAI	YACGTR	1 cut(s)	1112
BsaI	GGTCTC	1 cut(s)	628
BsaJI	CCNNGG	4 cut(s)	1405, 1450, 1716, 2247
BsaWI	WCCGGW	1 cut(s)	2073
BsaXI	ACNNNNNCTCC	2 cut(s)	57, 87
Bsc4I	CCNNNNNNNGG	2 cut(s)	112, 290
Bse118I	RCCGGY	1 cut(s)	1523
Bse1I	ACTGG	4 cut(s)	341, 1639, 1753, 2174
Bse3DI	GCAATG	3 cut(s)	174, 363, 1140
BseBI	CCWGG	4 cut(s)	1077, 1669, 1894, 2248
BseCI	ATCGAT	1 cut(s)	1209
BseDI	CCNNGG	4 cut(s)	1405, 1450, 1716, 2247
BseGI	GGATG	5 cut(s)	478, 874, 1121, 1242, 2082
BseLI	CCNNNNNNNGG	2 cut(s)	112, 290
BseMI	GCAATG	3 cut(s)	174, 363, 1140
BseMII	CTCAG	4 cut(s)	209, 219, 1153, 1569
BseNI	ACTGG	4 cut(s)	341, 1639, 1753, 2174
BseXI	GCAGC	6 cut(s)	145, 463, 809, 1627, 1915, 2068
BseYI	CCCAGC	3 cut(s)	106, 488, 1337
Bsh1236I	CGCG	1 cut(s)	323
BshFI	GGCC	3 cut(s)	1080, 1260, 1389
BshNI	GGYRCC	1 cut(s)	841
BshVI	ATCGAT	1 cut(s)	1209
BsiSI	CCGG	3 cut(s)	1081, 1524, 2074
BslFI	GGGAC	1 cut(s)	1246
BslI	CCNNNNNNNGG	2 cut(s)	112, 290
BsmAI	GTCTC	5 cut(s)	198, 199, 628, 690, 1465
BsmFI	GGGAC	1 cut(s)	1246
BsmI	GAATGC	4 cut(s)	1096, 1199, 1322, 1807
BsnI	GGCC	3 cut(s)	1080, 1260, 1389
Bso31I	GGTCTC	1 cut(s)	628
Bsp119I	TTCGAA	1 cut(s)	253
Bsp1286I	GDGCHC	1 cut(s)	2254
Bsp143I	GATC	7 cut(s)	210, 379, 1444, 1489, 1975, 2047, 2243
Bsp19I	CCATGG	1 cut(s)	1405
BspACI	CCGC	3 cut(s)	47, 1037, 2119
BspANI	GGCC	3 cut(s)	1080, 1260, 1389
BspCNI	CTCAG	4 cut(s)	208, 220, 1152, 1570
BspDI	ATCGAT	1 cut(s)	1209
BspFNI	CGCG	1 cut(s)	323
BspLI	GGNNCC	3 cut(s)	843, 1539, 1898
BspMAI	CTGCAG	1 cut(s)	1905
BspMI	ACCTGC	1 cut(s)	965
BspPI	GGATC	1 cut(s)	2238
BspQI	GCTCTTC	1 cut(s)	1128
BspT104I	TTCGAA	1 cut(s)	253
BspT107I	GGYRCC	1 cut(s)	841
BspTNI	GGTCTC	1 cut(s)	628
BsrBI	CCGCTC	1 cut(s)	2119
BsrDI	GCAATG	3 cut(s)	174, 363, 1140
BsrFI	RCCGGY	1 cut(s)	1523
BsrI	ACTGG	4 cut(s)	341, 1639, 1753, 2174
BssAI	RCCGGY	1 cut(s)	1523
BssECI	CCNNGG	4 cut(s)	1405, 1450, 1716, 2247
BssMI	GATC	7 cut(s)	210, 379, 1444, 1489, 1975, 2047, 2243
BssT1I	CCWWGG	2 cut(s)	1405, 1716
Bst2UI	CCWGG	4 cut(s)	1077, 1669, 1894, 2248
Bst4CI	ACNGT	6 cut(s)	190, 484, 521, 611, 735, 1454
Bst6I	CTCTTC	4 cut(s)	197, 1128, 1510, 1630
BstAPI	GCANNNNNTGC	1 cut(s)	1328
BstBAI	YACGTR	1 cut(s)	1112
BstBI	TTCGAA	1 cut(s)	253
BstC8I	GCNNGC	5 cut(s)	918, 1350, 1354, 1901, 2037
BstDEI	CTNAG	5 cut(s)	195, 228, 1139, 1578, 2126
BstDSI	CCRYGG	2 cut(s)	1405, 1450
BstF5I	GGATG	5 cut(s)	478, 874, 1121, 1242, 2082
BstFNI	CGCG	1 cut(s)	323
BstHHI	GCGC	2 cut(s)	323, 1121
BstKTI	GATC	7 cut(s)	213, 382, 1447, 1492, 1978, 2050, 2246
BstMAI	GTCTC	5 cut(s)	198, 199, 628, 690, 1465
BstMBI	GATC	7 cut(s)	210, 379, 1444, 1489, 1975, 2047, 2243
BstMWI	GCNNNNNNNGC	6 cut(s)	158, 164, 485, 1328, 1407, 1837
BstNI	CCWGG	4 cut(s)	1077, 1669, 1894, 2248
BstNSI	RCATGY	1 cut(s)	804
BstPAI	GACNNNNGTC	1 cut(s)	603
BstSCI	CCNGG	4 cut(s)	1075, 1667, 1892, 2246
BstSFI	CTRYAG	2 cut(s)	1509, 1901
BstUI	CGCG	1 cut(s)	323
BstV1I	GCAGC	6 cut(s)	145, 463, 809, 1627, 1915, 2068
BstV2I	GAAGAC	1 cut(s)	909
BstX2I	RGATCY	5 cut(s)	379, 1444, 1975, 2047, 2243
BstXI	CCANNNNNNTGG	1 cut(s)	2181
BstYI	RGATCY	5 cut(s)	379, 1444, 1975, 2047, 2243
Bsu15I	ATCGAT	1 cut(s)	1209
BsuRI	GGCC	3 cut(s)	1080, 1260, 1389
BsuTUI	ATCGAT	1 cut(s)	1209
BtgI	CCRYGG	2 cut(s)	1405, 1450
BtrI	CACGTC	1 cut(s)	461
BtsCI	GGATG	5 cut(s)	478, 874, 1121, 1242, 2082
BtsIMutI	CAGTG	2 cut(s)	607, 1548
BveI	ACCTGC	1 cut(s)	965
Cac8I	GCNNGC	5 cut(s)	918, 1350, 1354, 1901, 2037
CaiI	CAGNNNCTG	2 cut(s)	488, 1987
CfoI	GCGC	2 cut(s)	323, 1121
Cfr10I	RCCGGY	1 cut(s)	1523
Cfr13I	GGNCC	2 cut(s)	575, 1388
ClaI	ATCGAT	1 cut(s)	1209
CseI	GACGC	3 cut(s)	621, 1093, 1681
Csp6I	GTAC	5 cut(s)	313, 439, 522, 748, 763
CspCI	CAANNNNNGTGG	2 cut(s)	567, 602
CviAII	CATG	5 cut(s)	515, 801, 1166, 1406, 2234
CviQI	GTAC	5 cut(s)	313, 439, 522, 748, 763
DdeI	CTNAG	5 cut(s)	195, 228, 1139, 1578, 2126
DpnI	GATC	7 cut(s)	212, 381, 1446, 1491, 1977, 2049, 2245
DpnII	GATC	7 cut(s)	210, 379, 1444, 1489, 1975, 2047, 2243
EaeI	YGGCCR	1 cut(s)	1078
Eam1104I	CTCTTC	4 cut(s)	197, 1128, 1510, 1630
EarI	CTCTTC	4 cut(s)	197, 1128, 1510, 1630
Eco130I	CCWWGG	2 cut(s)	1405, 1716
Eco24I	GRGCYC	1 cut(s)	2254
Eco31I	GGTCTC	1 cut(s)	628
Eco47I	GGWCC	1 cut(s)	575
Eco57I	CTGAAG	4 cut(s)	297, 833, 906, 1964
Eco72I	CACGTG	1 cut(s)	1112
EcoRI	GAATTC	2 cut(s)	429, 2086
EcoRII	CCWGG	4 cut(s)	1075, 1667, 1892, 2246
EcoT14I	CCWWGG	2 cut(s)	1405, 1716
EcoT22I	ATGCAT	1 cut(s)	516
EcoT38I	GRGCYC	1 cut(s)	2254
ErhI	CCWWGG	2 cut(s)	1405, 1716
FaeI	CATG	5 cut(s)	518, 804, 1169, 1409, 2237
FaqI	GGGAC	1 cut(s)	1246
FatI	CATG	5 cut(s)	514, 800, 1165, 1405, 2233
Fnu4HI	GCNGC	6 cut(s)	159, 477, 798, 1616, 1904, 2082
FokI	GGATG	5 cut(s)	485, 881, 1128, 1229, 2089
FriOI	GRGCYC	1 cut(s)	2254
Fsp4HI	GCNGC	6 cut(s)	159, 477, 798, 1616, 1904, 2082
FspBI	CTAG	3 cut(s)	1026, 1044, 1676
GlaI	GCGC	2 cut(s)	322, 1120
GluI	GCNGC	6 cut(s)	159, 477, 798, 1616, 1904, 2082
GsaI	CCCAGC	3 cut(s)	110, 492, 1341
GsuI	CTGGAG	3 cut(s)	267, 1736, 1876
HaeIII	GGCC	3 cut(s)	1080, 1260, 1389
HapII	CCGG	3 cut(s)	1081, 1524, 2074
HgaI	GACGC	3 cut(s)	621, 1093, 1681
HhaI	GCGC	2 cut(s)	323, 1121
Hin1II	CATG	5 cut(s)	518, 804, 1169, 1409, 2237
Hin6I	GCGC	2 cut(s)	321, 1119
HinP1I	GCGC	2 cut(s)	321, 1119
HincII	GTYRAC	1 cut(s)	2170
HindII	GTYRAC	1 cut(s)	2170
HindIII	AAGCTT	3 cut(s)	682, 1838, 1858
HpaII	CCGG	3 cut(s)	1081, 1524, 2074
HphI	GGTGA	5 cut(s)	64, 137, 211, 1492, 1652
Hpy166II	GTNNAC	3 cut(s)	550, 763, 2170
Hpy188I	TCNGA	7 cut(s)	277, 415, 970, 1160, 1732, 1983, 2243
Hpy188III	TCNNGA	8 cut(s)	284, 398, 1066, 1175, 1364, 1739, 1874, 2223
Hpy8I	GTNNAC	3 cut(s)	550, 763, 2170
Hpy99I	CGWCG	1 cut(s)	1299
HpyAV	CCTTC	2 cut(s)	260, 769
HpyCH4III	ACNGT	6 cut(s)	190, 484, 521, 611, 735, 1454
HpyCH4IV	ACGT	5 cut(s)	97, 244, 460, 1111, 1818
HpyF10VI	GCNNNNNNNGC	6 cut(s)	158, 164, 485, 1328, 1407, 1837
HpyF3I	CTNAG	5 cut(s)	195, 228, 1139, 1578, 2126
HpySE526I	ACGT	5 cut(s)	97, 244, 460, 1111, 1818
Hsp92II	CATG	5 cut(s)	518, 804, 1169, 1409, 2237
HspAI	GCGC	2 cut(s)	321, 1119
Kzo9I	GATC	7 cut(s)	210, 379, 1444, 1489, 1975, 2047, 2243
LguI	GCTCTTC	1 cut(s)	1128
LmnI	GCTCC	7 cut(s)	286, 742, 1006, 1222, 1406, 1896, 2116
Lsp1109I	GCAGC	6 cut(s)	145, 463, 809, 1627, 1915, 2068
LweI	GCATC	9 cut(s)	463, 501, 903, 961, 1106, 1251, 1818, 1896, 2216
MaeI	CTAG	3 cut(s)	1026, 1044, 1676
MaeII	ACGT	5 cut(s)	97, 244, 460, 1111, 1818
MaeIII	GTNAC	4 cut(s)	245, 349, 605, 1558
MalI	GATC	7 cut(s)	212, 381, 1446, 1491, 1977, 2049, 2245
MbiI	CCGCTC	1 cut(s)	2119
MboI	GATC	7 cut(s)	210, 379, 1444, 1489, 1975, 2047, 2243
MfeI	CAATTG	1 cut(s)	1998
MflI	RGATCY	5 cut(s)	379, 1444, 1975, 2047, 2243
MhlI	GDGCHC	1 cut(s)	2254
MlyI	GAGTC	6 cut(s)	319, 1164, 1172, 1496, 1820, 2144
MmeI	TCCRAC	4 cut(s)	4, 602, 912, 1788
Mph1103I	ATGCAT	1 cut(s)	516
MroXI	GAANNNNTTC	2 cut(s)	1322, 1977
MslI	CAYNNNNRTG	4 cut(s)	540, 1114, 1497, 1635
MspA1I	CMGCKG	2 cut(s)	110, 1037
MspI	CCGG	3 cut(s)	1081, 1524, 2074
MspR9I	CCNGG	4 cut(s)	1077, 1669, 1894, 2248
MunI	CAATTG	1 cut(s)	1998
Mva1269I	GAATGC	4 cut(s)	1096, 1199, 1322, 1807
MvaI	CCWGG	4 cut(s)	1077, 1669, 1894, 2248
MvnI	CGCG	1 cut(s)	323
MwoI	GCNNNNNNNGC	6 cut(s)	158, 164, 485, 1328, 1407, 1837
NcoI	CCATGG	1 cut(s)	1405
NdeII	GATC	7 cut(s)	210, 379, 1444, 1489, 1975, 2047, 2243
NlaIII	CATG	5 cut(s)	518, 804, 1169, 1409, 2237
NlaIV	GGNNCC	3 cut(s)	843, 1539, 1898
NmuCI	GTSAC	2 cut(s)	245, 605
NsiI	ATGCAT	1 cut(s)	516
NspI	RCATGY	1 cut(s)	804
NspV	TTCGAA	1 cut(s)	253
PciSI	GCTCTTC	1 cut(s)	1128
PcsI	WCGNNNNNNNCGW	2 cut(s)	250, 1091
PctI	GAATGC	4 cut(s)	1096, 1199, 1322, 1807
PdmI	GAANNNNTTC	2 cut(s)	1322, 1977
PfeI	GAWTC	6 cut(s)	89, 301, 394, 994, 1030, 1469
PflFI	GACNNNGTC	1 cut(s)	2174
PflMI	CCANNNNNTGG	1 cut(s)	290
PfoI	TCCNGGA	1 cut(s)	1892
PkrI	GCNGC	6 cut(s)	160, 478, 799, 1617, 1905, 2083
PleI	GAGTC	6 cut(s)	319, 1163, 1172, 1496, 1819, 2144
PmaCI	CACGTG	1 cut(s)	1112
PmlI	CACGTG	1 cut(s)	1112
PpsI	GAGTC	6 cut(s)	319, 1163, 1172, 1496, 1819, 2144
Ppu21I	YACGTR	1 cut(s)	1112
PshAI	GACNNNNGTC	1 cut(s)	603
Psp6I	CCWGG	4 cut(s)	1075, 1667, 1892, 2246
PspCI	CACGTG	1 cut(s)	1112
PspFI	CCCAGC	3 cut(s)	106, 488, 1337
PspGI	CCWGG	4 cut(s)	1075, 1667, 1892, 2246
PspN4I	GGNNCC	3 cut(s)	843, 1539, 1898
PspPI	GGNCC	2 cut(s)	575, 1388
PstI	CTGCAG	1 cut(s)	1905
PstNI	CAGNNNCTG	2 cut(s)	488, 1987
PsuI	RGATCY	5 cut(s)	379, 1444, 1975, 2047, 2243
PsyI	GACNNNGTC	1 cut(s)	2174
PvuII	CAGCTG	1 cut(s)	110
RsaI	GTAC	5 cut(s)	314, 440, 523, 749, 764
RsaNI	GTAC	5 cut(s)	313, 439, 522, 748, 763
RseI	CAYNNNNRTG	4 cut(s)	540, 1114, 1497, 1635
SapI	GCTCTTC	1 cut(s)	1128
SatI	GCNGC	6 cut(s)	159, 477, 798, 1616, 1904, 2082
Sau3AI	GATC	7 cut(s)	210, 379, 1444, 1489, 1975, 2047, 2243
Sau96I	GGNCC	2 cut(s)	575, 1388
ScaI	AGTACT	1 cut(s)	314
SchI	GAGTC	6 cut(s)	319, 1164, 1172, 1496, 1820, 2144
ScrFI	CCNGG	4 cut(s)	1077, 1669, 1894, 2248
SduI	GDGCHC	1 cut(s)	2254
SfaNI	GCATC	9 cut(s)	463, 501, 903, 961, 1106, 1251, 1818, 1896, 2216
SfcI	CTRYAG	2 cut(s)	1509, 1901
SfuI	TTCGAA	1 cut(s)	253
SinI	GGWCC	1 cut(s)	575
SmiMI	CAYNNNNRTG	4 cut(s)	540, 1114, 1497, 1635
SmlI	CTYRAG	1 cut(s)	1737
SmoI	CTYRAG	1 cut(s)	1737
SsiI	CCGC	3 cut(s)	47, 1037, 2119
SspI	AATATT	2 cut(s)	853, 1050
SspMI	CTAG	3 cut(s)	1026, 1044, 1676
StyD4I	CCNGG	4 cut(s)	1075, 1667, 1892, 2246
StyI	CCWWGG	2 cut(s)	1405, 1716
TaaI	ACNGT	6 cut(s)	190, 484, 521, 611, 735, 1454
TaiI	ACGT	5 cut(s)	100, 247, 463, 1114, 1821
TaqI	TCGA	8 cut(s)	92, 253, 702, 1153, 1209, 1294, 1855, 2222
TatI	WGTACW	4 cut(s)	312, 521, 747, 762
TfiI	GAWTC	6 cut(s)	89, 301, 394, 994, 1030, 1469
TscAI	CASTG	2 cut(s)	614, 1555
TseFI	GTSAC	2 cut(s)	245, 605
TseI	GCWGC	6 cut(s)	158, 476, 797, 1615, 1903, 2081
Tsp45I	GTSAC	2 cut(s)	245, 605
TspDTI	ATGAA	9 cut(s)	102, 188, 293, 668, 885, 986, 1227, 1301, 1956
TspGWI	ACGGA	2 cut(s)	851, 1509
TspRI	CASTG	2 cut(s)	614, 1555
Tth111I	GACNNNGTC	1 cut(s)	2174
Van91I	CCANNNNNTGG	1 cut(s)	290
VpaK11BI	GGWCC	1 cut(s)	575
XapI	RAATTY	4 cut(s)	221, 255, 429, 2086
XceI	RCATGY	1 cut(s)	804
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1403
XmnI	GAANNNNTTC	2 cut(s)	1322, 1977
XspI	CTAG	3 cut(s)	1026, 1044, 1676
ZrmI	AGTACT	1 cut(s)	314
Zsp2I	ATGCAT	1 cut(s)	516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.