Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005489

Physical Location & Seq

Genomic Coordinates Reverse (-)

27611916 .. 27617753

Gene Structure 5838 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1201800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGAGTGTGGTGGGGTTTGATGTTGGAAATGAGAATTGTGTAATTGCGGTGGTGAAGCAACGGGGAGTTGATGTTTTGTTGAATGATGAATCGAAACGTGAAACCCCCGCTGTGGTTTGTTTTGGTGAGAAGCAGAGGTTTCTGGGGTCTGCTGGTGCTGCCTCTGCAATGATGAACCCGAAATCTACGGTGTCTCAGGTGAAGAGACTGATTGGTAGAAAATTTACTGAGCCTGATGTTCAACGTGACCTTCAAATTTTACCCCTTGATACTTCTGAAGCTCCAGATGGTGGCATTTTGATTCATTTGAAGTACTTGGGGGCGACTCATACATTTACGCCCGTTCAAGTTACAGCAATGCTTTTTGCACATTTGAAAGATCTAATAGAGAAGAATCAGGAAATGCCCATTTCAGATTGTGTGATTGGAATTCCATCGTACTTTACAGACTTGCAAAGACGTGCATATTTGGATGCTGCCACAGTTGCTGGGTTGAAGCCTTTGAGATTGATGCATGACTGTACTGCAACTGCTCTTAGTTATGGGATTTACAAAACAGATTTCTCAACTTCTGGTCCAACTTATGTGGCATTTATTGACATTGGTCACTGTGATACACAGGTCTCTATTGCGTCATTTGAGGCTGGGGAAATGAAGATACTATCTCATACTTTTGATAGAAGCTTGGGAGGGAGAGACTTCGATGAGATCTTGTTTAATCATTTTGCTGCACAGTTCAAGGAGCAGTACAGAATTGATGTTTACTCTAATGTCAAGGCAAGTATCAGGTTACGGGCAGCATGTGAGAAGCTGAAGAAAGTGTTGAGTGCGAATGCGGAGGCACCTCTTAATATTGAGTGCTTAATGGATGAAATAGATGTCAGGGGATTTATTAAAAGGGAAGACTTTGAGATGCTGGCTTCAGGTTTGTTGGAGAGGATTGGTATTCCTTGTAGCAAAGCTTTAGCTGATGCAGGTTTGACTGCTGATAAGATTCATTCTGTGGAGCTTGTCGGGTCAGGGTCTAGGATTCCAGCGGTTGCTAGAATATTAGCTTCTGTGTTCAGGAAAGAACCCGGCCGGACGCTGAATGCGAGTGAGTGTGTAGCACGTGGATGCGCTCTTCAATGTGCAATGCTCAGTCCTGTTTTTCGAGTCAGAGAATATGAGGTTCAAGACTCAATTCCTTTCTCGGTAGCATTCTTAATAGATGAAGCTCCAATTTGCACAGGGACAAGTGGCATCCTGTTTCCAAAAGGCCAACCCATTCCAAGTGTTAAAGTACTGACGTTTCGACGAAGCAGTTTATTTCATTTGAAAGCATTCTATGCTAATCCCAGTGAAGTGCCTGCTGGCACTTCTCCTGACATTTGTTGTTTTAAGATTGGCCCTTTCCAATGCTCCCATGGCGAAAAAACAAGGGTTAAAGTTAAAGTTAAAGTTATGTTGGATCTCCACGGTGTTGTCTCTGTTGAATCAGCTATGGTGATGGAAGATCACGGAGATGACTCTTCTACAAGGGGTCTTGCCGGTTCAAAAATGGAACCAATGGACACTGATTATGTAACTGCTTCTGGTTCTACTGAGGCAGTTGCAGATGGATTTGAAGAAAGCAGCATGCAGCACAACTCTTCACATACTGGTGGTGAGGCTAAAAGAAACAAAAAACCCACCAGGCGGCTAGAGATACCTGTTAGTGAAAGTATATATGGTCGAATGACCAAGGCTGAACTTTCAGAAGCTCAAGAAAAAGAACTCCAGTTAGCTCAACAGGACAGAATTATGGAACAAACCAAAGACAAAAAGAATGCGTTGGAGTCTTACGTCTATGAGATGCGAAATAAGCTTCTCAACACATATCGAAGCTTTGCAAGTGATCAAGAGAGGGAGGGCATCTCCAGGAGCCTGCAGCAGACAGAGGAATGGCTTTATGATGATGGAGAAGATGAAACAGAAAATGCTTATACTTCAAAGCTGGAAGATCTTCAGAAGCTGGTAGATCCAATTGAGAACCGATATAAAGATGAGGAAGCAAGATTACAAGCTACAAGAGATCTGTTGAAGTGCACTGGGGATTACCGGATGGCTGTGAATTCACTTCCACTCATGGATAGAGAATCGGTTGTTAATGAGTGCTATAAAGTGGAGCAGTGGCTTAGGGACAAAAACCAACAACAAGACTCATTGCCAAAGAATGTTGACCCAGTCTTATGGTCAAATGATATTAGGAGCAGGAATGAGGAATTGAAGTCGAGATGCAACCATGTATTCAGATCCAGGGCTCCTAATCGCGAGGACCACAAGGGCTCTAACCAACATGACACTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04530 ko04612 map04530 map04612

Pfam Domains

Protein Families

HSP70

Protein Analysis

777

Amino Acids

86.61

Weight (kDa)

5.72

Isoelectric Point (pI)

44.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	3 - 673	1.3e-141	Hsp70 protein
MreB_Mbl	PF06723	116 - 375	1e-07	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005489)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11660 AT1G11660 AT1G11660 AT1G11660
fragaria_vesca	FvH4_3g22620 FvH4_3g22620 FvH4_3g22620 FvH4_3g22620 FvH4_3g22620
malus_domestica	MD03G1201700.v1.1 MD03G1201800.v1.1 MD11G1218900.v1.1
prunus_persica	Prupe.4G212200_v2.0.a1
pyrus_communis	pycom03g15300 pycom03g15320 pycom11g19290
rosa_chinensis	RchiOBHm_Chr5g0039371
rosa_laevigata	RLG00000033908
rosa_multiflora	Rmu_co8453851.1_g000001 Rmu_sc0006937.1_g000008 Rmu_sc0007310.1_g000013
rosa_roxburghii	Rroxscaffold_1G00040520 Rroxscaffold_1G00041830
rosa_rugosa	Rorug05G0178400
rosa_samantha	Rh5AG264600 Rh5BG269600 Rh5CG302600 Rh5DG277400
rosa_wichuraiana	Rw5G024820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	965
AccB1I	GGYRCC	1 cut(s)	841
AccB7I	CCANNNNNTGG	1 cut(s)	290
AccII	CGCG	1 cut(s)	2292
AciI	CCGC	5 cut(s)	47, 108, 836, 1037, 1678
AclWI	GGATC	3 cut(s)	1458, 1994, 2268
AcoI	YGGCCR	1 cut(s)	1078
AcsI	RAATTY	4 cut(s)	221, 255, 429, 2092
AcuI	CTGAAG	4 cut(s)	297, 833, 906, 1970
AcvI	CACGTG	1 cut(s)	1112
AfaI	GTAC	5 cut(s)	314, 440, 523, 749, 1284
AfiI	CCNNNNNNNGG	3 cut(s)	112, 290, 1677
AjiI	CACGTC	1 cut(s)	461
AjnI	CCWGG	3 cut(s)	1673, 1898, 2276
Alw21I	GWGCWC	1 cut(s)	2069
Alw26I	GTCTC	5 cut(s)	198, 199, 628, 690, 1471
Alw44I	GTGCAC	1 cut(s)	2065
AlwI	GGATC	3 cut(s)	1458, 1994, 2268
AlwNI	CAGNNNCTG	2 cut(s)	488, 1993
AoxI	GGCC	3 cut(s)	1078, 1258, 1387
ApaLI	GTGCAC	1 cut(s)	2065
ApeKI	GCWGC	7 cut(s)	158, 476, 728, 797, 1614, 1621, 1909
ApoI	RAATTY	4 cut(s)	221, 255, 429, 2092
Asp700I	GAANNNNTTC	2 cut(s)	1322, 1983
AspLEI	GCGC	1 cut(s)	1121
AspS9I	GGNCC	3 cut(s)	575, 1388, 2296
AsuC2I	CCSGG	1 cut(s)	1077
AsuHPI	GGTGA	5 cut(s)	64, 137, 211, 1498, 1658
AvaII	GGWCC	2 cut(s)	575, 2296
BaeGI	GKGCMC	1 cut(s)	2069
BaeI	ACNNNNGTAYC	2 cut(s)	606, 639
BanI	GGYRCC	1 cut(s)	841
BanII	GRGCYC	2 cut(s)	2284, 2309
BarI	GAAGNNNNNNTAC	2 cut(s)	2022, 2054
BbrPI	CACGTG	1 cut(s)	1112
BbsI	GAAGAC	1 cut(s)	909
Bbv12I	GWGCWC	1 cut(s)	2069
BbvI	GCAGC	7 cut(s)	145, 463, 715, 809, 1626, 1633, 1921
BccI	CCATC	6 cut(s)	281, 442, 1483, 1592, 1931, 2077
BciT130I	CCWGG	3 cut(s)	1675, 1900, 2278
BclI	TGATCA	1 cut(s)	1876
BcnI	CCSGG	1 cut(s)	1077
BcoDI	GTCTC	5 cut(s)	198, 199, 628, 690, 1471
BfaI	CTAG	3 cut(s)	1026, 1044, 1682
BfmI	CTRYAG	1 cut(s)	1907
BfuAI	ACCTGC	1 cut(s)	965
BglII	AGATCT	4 cut(s)	379, 708, 1981, 2053
BisI	GCNGC	8 cut(s)	159, 477, 729, 798, 1615, 1622, 1679, 1910
BlsI	GCNGC	8 cut(s)	160, 478, 730, 799, 1616, 1623, 1680, 1911
BmcAI	AGTACT	2 cut(s)	314, 1284
Bme1390I	CCNGG	4 cut(s)	1077, 1675, 1900, 2278
Bme18I	GGWCC	2 cut(s)	575, 2296
BmgBI	CACGTC	1 cut(s)	461
BmgT120I	GGNCC	3 cut(s)	575, 1388, 2296
BmiI	GGNNCC	4 cut(s)	843, 1545, 1904, 2283
BmrFI	CCNGG	4 cut(s)	1077, 1675, 1900, 2278
BmrI	ACTGGG	3 cut(s)	1332, 2079, 2198
BmsI	GCATC	9 cut(s)	463, 501, 903, 961, 1106, 1251, 1824, 1902, 2246
BmuI	ACTGGG	3 cut(s)	1332, 2079, 2198
BoxI	GACNNNNGTC	1 cut(s)	603
BpiI	GAAGAC	1 cut(s)	909
BplI	GAGNNNNNCTC	2 cut(s)	1880, 1912
BpmI	CTGGAG	3 cut(s)	267, 1742, 1882
Bpu10I	CCTNAGC	1 cut(s)	2156
BpuEI	CTTGAG	1 cut(s)	1728
BpuMI	CCSGG	1 cut(s)	1077
BsaAI	YACGTR	1 cut(s)	1112
BsaI	GGTCTC	1 cut(s)	628
BsaJI	CCNNGG	4 cut(s)	1405, 1456, 1722, 2277
BsaWI	WCCGGW	1 cut(s)	2079
BsaXI	ACNNNNNCTCC	2 cut(s)	57, 87
Bsc4I	CCNNNNNNNGG	3 cut(s)	112, 290, 1677
Bse118I	RCCGGY	1 cut(s)	1529
Bse1I	ACTGG	5 cut(s)	1338, 1645, 1759, 2074, 2204
Bse3DI	GCAATG	4 cut(s)	174, 363, 1140, 2183
BseBI	CCWGG	3 cut(s)	1675, 1900, 2278
BseDI	CCNNGG	4 cut(s)	1405, 1456, 1722, 2277
BseGI	GGATG	5 cut(s)	478, 874, 1121, 1242, 2088
BseLI	CCNNNNNNNGG	3 cut(s)	112, 290, 1677
BseMI	GCAATG	4 cut(s)	174, 363, 1140, 2183
BseMII	CTCAG	4 cut(s)	209, 219, 1153, 1575
BseNI	ACTGG	5 cut(s)	1338, 1645, 1759, 2074, 2204
BseSI	GKGCMC	1 cut(s)	2069
BseX3I	CGGCCG	1 cut(s)	1078
BseXI	GCAGC	7 cut(s)	145, 463, 715, 809, 1626, 1633, 1921
BseYI	CCCAGC	2 cut(s)	488, 644
BsgI	GTGCAG	1 cut(s)	714
Bsh1236I	CGCG	1 cut(s)	2292
Bsh1285I	CGRYCG	1 cut(s)	1081
BshFI	GGCC	3 cut(s)	1080, 1260, 1389
BshNI	GGYRCC	1 cut(s)	841
BsiEI	CGRYCG	1 cut(s)	1081
BsiHKAI	GWGCWC	1 cut(s)	2069
BsiSI	CCGG	4 cut(s)	1077, 1081, 1530, 2080
BslFI	GGGAC	2 cut(s)	1246, 2174
BslI	CCNNNNNNNGG	3 cut(s)	112, 290, 1677
BsmAI	GTCTC	5 cut(s)	198, 199, 628, 690, 1471
BsmFI	GGGAC	2 cut(s)	1246, 2174
BsmI	GAATGC	5 cut(s)	838, 1096, 1199, 1322, 1813
BsnI	GGCC	3 cut(s)	1080, 1260, 1389
Bso31I	GGTCTC	1 cut(s)	628
Bsp1286I	GDGCHC	3 cut(s)	2069, 2284, 2309
Bsp143I	GATC	9 cut(s)	379, 708, 1450, 1495, 1876, 1981, 1999, 2053, 2273
Bsp19I	CCATGG	1 cut(s)	1405
Bsp68I	TCGCGA	1 cut(s)	2292
BspACI	CCGC	5 cut(s)	47, 108, 836, 1037, 1678
BspANI	GGCC	3 cut(s)	1080, 1260, 1389
BspCNI	CTCAG	4 cut(s)	208, 220, 1152, 1576
BspFNI	CGCG	1 cut(s)	2292
BspLI	GGNNCC	4 cut(s)	843, 1545, 1904, 2283
BspMAI	CTGCAG	1 cut(s)	1911
BspMI	ACCTGC	1 cut(s)	965
BspPI	GGATC	3 cut(s)	1458, 1994, 2268
BspQI	GCTCTTC	1 cut(s)	1128
BspT107I	GGYRCC	1 cut(s)	841
BspTNI	GGTCTC	1 cut(s)	628
BsrDI	GCAATG	4 cut(s)	174, 363, 1140, 2183
BsrFI	RCCGGY	1 cut(s)	1529
BsrI	ACTGG	5 cut(s)	1338, 1645, 1759, 2074, 2204
BssAI	RCCGGY	1 cut(s)	1529
BssECI	CCNNGG	4 cut(s)	1405, 1456, 1722, 2277
BssMI	GATC	9 cut(s)	379, 708, 1450, 1495, 1876, 1981, 1999, 2053, 2273
BssT1I	CCWWGG	2 cut(s)	1405, 1722
Bst2UI	CCWGG	3 cut(s)	1675, 1900, 2278
Bst4CI	ACNGT	6 cut(s)	190, 484, 521, 611, 735, 1460
Bst6I	CTCTTC	4 cut(s)	197, 1128, 1516, 1636
BstAPI	GCANNNNNTGC	1 cut(s)	1328
BstBAI	YACGTR	1 cut(s)	1112
BstC8I	GCNNGC	5 cut(s)	918, 1350, 1354, 1619, 1907
BstDEI	CTNAG	6 cut(s)	195, 228, 536, 1139, 1584, 2156
BstDSI	CCRYGG	2 cut(s)	1405, 1456
BstF5I	GGATG	5 cut(s)	478, 874, 1121, 1242, 2088
BstFNI	CGCG	1 cut(s)	2292
BstHHI	GCGC	1 cut(s)	1121
BstKTI	GATC	9 cut(s)	382, 711, 1453, 1498, 1879, 1984, 2002, 2056, 2276
BstMAI	GTCTC	5 cut(s)	198, 199, 628, 690, 1471
BstMBI	GATC	9 cut(s)	379, 708, 1450, 1495, 1876, 1981, 1999, 2053, 2273
BstMCI	CGRYCG	1 cut(s)	1081
BstMWI	GCNNNNNNNGC	6 cut(s)	158, 164, 485, 1328, 1407, 1843
BstNI	CCWGG	3 cut(s)	1675, 1900, 2278
BstNSI	RCATGY	2 cut(s)	804, 1621
BstPAI	GACNNNNGTC	1 cut(s)	603
BstSCI	CCNGG	4 cut(s)	1075, 1673, 1898, 2276
BstSFI	CTRYAG	1 cut(s)	1907
BstSLI	GKGCMC	1 cut(s)	2069
BstUI	CGCG	1 cut(s)	2292
BstV1I	GCAGC	7 cut(s)	145, 463, 715, 809, 1626, 1633, 1921
BstV2I	GAAGAC	1 cut(s)	909
BstX2I	RGATCY	7 cut(s)	379, 708, 1450, 1981, 1999, 2053, 2273
BstXI	CCANNNNNNTGG	1 cut(s)	2211
BstYI	RGATCY	7 cut(s)	379, 708, 1450, 1981, 1999, 2053, 2273
BstZI	CGGCCG	1 cut(s)	1078
BsuRI	GGCC	3 cut(s)	1080, 1260, 1389
BtgI	CCRYGG	2 cut(s)	1405, 1456
BtrI	CACGTC	1 cut(s)	461
BtsCI	GGATG	5 cut(s)	478, 874, 1121, 1242, 2088
BtsI	GCAGTG	1 cut(s)	2156
BtsIMutI	CAGTG	5 cut(s)	607, 1345, 1554, 2067, 2156
BtuMI	TCGCGA	1 cut(s)	2292
BveI	ACCTGC	1 cut(s)	965
Cac8I	GCNNGC	5 cut(s)	918, 1350, 1354, 1619, 1907
CaiI	CAGNNNCTG	2 cut(s)	488, 1993
CfoI	GCGC	1 cut(s)	1121
Cfr10I	RCCGGY	1 cut(s)	1529
Cfr13I	GGNCC	3 cut(s)	575, 1388, 2296
CseI	GACGC	2 cut(s)	621, 1093
Csp6I	GTAC	5 cut(s)	313, 439, 522, 748, 1283
CspCI	CAANNNNNGTGG	2 cut(s)	567, 602
CviAII	CATG	7 cut(s)	515, 801, 1406, 1618, 2107, 2264, 2318
CviQI	GTAC	5 cut(s)	313, 439, 522, 748, 1283
DdeI	CTNAG	6 cut(s)	195, 228, 536, 1139, 1584, 2156
DpnI	GATC	9 cut(s)	381, 710, 1452, 1497, 1878, 1983, 2001, 2055, 2275
DpnII	GATC	9 cut(s)	379, 708, 1450, 1495, 1876, 1981, 1999, 2053, 2273
EaeI	YGGCCR	1 cut(s)	1078
EagI	CGGCCG	1 cut(s)	1078
Eam1104I	CTCTTC	4 cut(s)	197, 1128, 1516, 1636
EarI	CTCTTC	4 cut(s)	197, 1128, 1516, 1636
EclXI	CGGCCG	1 cut(s)	1078
Eco130I	CCWWGG	2 cut(s)	1405, 1722
Eco24I	GRGCYC	2 cut(s)	2284, 2309
Eco31I	GGTCTC	1 cut(s)	628
Eco47I	GGWCC	2 cut(s)	575, 2296
Eco52I	CGGCCG	1 cut(s)	1078
Eco57I	CTGAAG	4 cut(s)	297, 833, 906, 1970
Eco72I	CACGTG	1 cut(s)	1112
EcoRI	GAATTC	2 cut(s)	429, 2092
EcoRII	CCWGG	3 cut(s)	1673, 1898, 2276
EcoT14I	CCWWGG	2 cut(s)	1405, 1722
EcoT22I	ATGCAT	1 cut(s)	516
EcoT38I	GRGCYC	2 cut(s)	2284, 2309
ErhI	CCWWGG	2 cut(s)	1405, 1722
FaeI	CATG	7 cut(s)	518, 804, 1409, 1621, 2110, 2267, 2321
FaqI	GGGAC	2 cut(s)	1246, 2174
FatI	CATG	7 cut(s)	514, 800, 1405, 1617, 2106, 2263, 2317
FauI	CCCGC	1 cut(s)	115
FbaI	TGATCA	1 cut(s)	1876
Fnu4HI	GCNGC	8 cut(s)	159, 477, 729, 798, 1615, 1622, 1679, 1910
FokI	GGATG	5 cut(s)	485, 881, 1128, 1229, 2095
FriOI	GRGCYC	2 cut(s)	2284, 2309
Fsp4HI	GCNGC	8 cut(s)	159, 477, 729, 798, 1615, 1622, 1679, 1910
FspBI	CTAG	3 cut(s)	1026, 1044, 1682
GlaI	GCGC	1 cut(s)	1120
GluI	GCNGC	8 cut(s)	159, 477, 729, 798, 1615, 1622, 1679, 1910
GsaI	CCCAGC	2 cut(s)	492, 648
GsuI	CTGGAG	3 cut(s)	267, 1742, 1882
HaeIII	GGCC	3 cut(s)	1080, 1260, 1389
HapII	CCGG	4 cut(s)	1077, 1081, 1530, 2080
HgaI	GACGC	2 cut(s)	621, 1093
HhaI	GCGC	1 cut(s)	1121
Hin1II	CATG	7 cut(s)	518, 804, 1409, 1621, 2110, 2267, 2321
Hin6I	GCGC	1 cut(s)	1119
HinP1I	GCGC	1 cut(s)	1119
HincII	GTYRAC	1 cut(s)	2200
HindII	GTYRAC	1 cut(s)	2200
HindIII	AAGCTT	4 cut(s)	682, 960, 1844, 1864
HpaII	CCGG	4 cut(s)	1077, 1081, 1530, 2080
HphI	GGTGA	5 cut(s)	64, 137, 211, 1498, 1658
Hpy166II	GTNNAC	3 cut(s)	763, 2067, 2200
Hpy188I	TCNGA	7 cut(s)	277, 415, 1160, 1738, 1989, 2273, 2330
Hpy188III	TCNNGA	9 cut(s)	284, 398, 1066, 1175, 1364, 1745, 1880, 2253, 2291
Hpy8I	GTNNAC	3 cut(s)	763, 2067, 2200
Hpy99I	CGWCG	1 cut(s)	1299
HpyAV	CCTTC	1 cut(s)	260
HpyCH4III	ACNGT	6 cut(s)	190, 484, 521, 611, 735, 1460
HpyCH4IV	ACGT	6 cut(s)	97, 244, 460, 1111, 1289, 1824
HpyF10VI	GCNNNNNNNGC	6 cut(s)	158, 164, 485, 1328, 1407, 1843
HpyF3I	CTNAG	6 cut(s)	195, 228, 536, 1139, 1584, 2156
HpySE526I	ACGT	6 cut(s)	97, 244, 460, 1111, 1289, 1824
Hsp92II	CATG	7 cut(s)	518, 804, 1409, 1621, 2110, 2267, 2321
HspAI	GCGC	1 cut(s)	1119
Ksp22I	TGATCA	1 cut(s)	1876
Kzo9I	GATC	9 cut(s)	379, 708, 1450, 1495, 1876, 1981, 1999, 2053, 2273
LguI	GCTCTTC	1 cut(s)	1128
LmnI	GCTCC	9 cut(s)	286, 742, 1006, 1222, 1406, 1902, 2146, 2229, 2287
Lsp1109I	GCAGC	7 cut(s)	145, 463, 715, 809, 1626, 1633, 1921
LweI	GCATC	9 cut(s)	463, 501, 903, 961, 1106, 1251, 1824, 1902, 2246
MaeI	CTAG	3 cut(s)	1026, 1044, 1682
MaeII	ACGT	6 cut(s)	97, 244, 460, 1111, 1289, 1824
MaeIII	GTNAC	5 cut(s)	245, 349, 605, 789, 1564
MalI	GATC	9 cut(s)	381, 710, 1452, 1497, 1878, 1983, 2001, 2055, 2275
MboI	GATC	9 cut(s)	379, 708, 1450, 1495, 1876, 1981, 1999, 2053, 2273
MfeI	CAATTG	1 cut(s)	2004
MflI	RGATCY	7 cut(s)	379, 708, 1450, 1981, 1999, 2053, 2273
MhlI	GDGCHC	3 cut(s)	2069, 2284, 2309
MlyI	GAGTC	6 cut(s)	319, 1164, 1172, 1502, 1826, 2174
MmeI	TCCRAC	5 cut(s)	4, 602, 912, 1428, 1794
Mph1103I	ATGCAT	1 cut(s)	516
MroXI	GAANNNNTTC	2 cut(s)	1322, 1983
MslI	CAYNNNNRTG	3 cut(s)	1114, 1503, 1641
MspA1I	CMGCKG	2 cut(s)	110, 1037
MspI	CCGG	4 cut(s)	1077, 1081, 1530, 2080
MspR9I	CCNGG	4 cut(s)	1077, 1675, 1900, 2278
MunI	CAATTG	1 cut(s)	2004
Mva1269I	GAATGC	5 cut(s)	838, 1096, 1199, 1322, 1813
MvaI	CCWGG	3 cut(s)	1675, 1900, 2278
MvnI	CGCG	1 cut(s)	2292
MwoI	GCNNNNNNNGC	6 cut(s)	158, 164, 485, 1328, 1407, 1843
NciI	CCSGG	1 cut(s)	1077
NcoI	CCATGG	1 cut(s)	1405
NdeII	GATC	9 cut(s)	379, 708, 1450, 1495, 1876, 1981, 1999, 2053, 2273
NlaIII	CATG	7 cut(s)	518, 804, 1409, 1621, 2110, 2267, 2321
NlaIV	GGNNCC	4 cut(s)	843, 1545, 1904, 2283
NmuCI	GTSAC	2 cut(s)	245, 605
NruI	TCGCGA	1 cut(s)	2292
NsiI	ATGCAT	1 cut(s)	516
NspI	RCATGY	2 cut(s)	804, 1621
PaeI	GCATGC	1 cut(s)	1621
PciSI	GCTCTTC	1 cut(s)	1128
PcsI	WCGNNNNNNNCGW	1 cut(s)	1091
PctI	GAATGC	5 cut(s)	838, 1096, 1199, 1322, 1813
PdmI	GAANNNNTTC	2 cut(s)	1322, 1983
PfeI	GAWTC	7 cut(s)	89, 301, 394, 994, 1030, 1475, 2117
PflFI	GACNNNGTC	1 cut(s)	2204
PflMI	CCANNNNNTGG	1 cut(s)	290
PfoI	TCCNGGA	1 cut(s)	1898
PkrI	GCNGC	8 cut(s)	160, 478, 730, 799, 1616, 1623, 1680, 1911
PleI	GAGTC	6 cut(s)	319, 1163, 1172, 1502, 1825, 2174
PmaCI	CACGTG	1 cut(s)	1112
PmlI	CACGTG	1 cut(s)	1112
PpsI	GAGTC	6 cut(s)	319, 1163, 1172, 1502, 1825, 2174
Ppu21I	YACGTR	1 cut(s)	1112
PshAI	GACNNNNGTC	1 cut(s)	603
Psp6I	CCWGG	3 cut(s)	1673, 1898, 2276
PspCI	CACGTG	1 cut(s)	1112
PspFI	CCCAGC	2 cut(s)	488, 644
PspGI	CCWGG	3 cut(s)	1673, 1898, 2276
PspN4I	GGNNCC	4 cut(s)	843, 1545, 1904, 2283
PspPI	GGNCC	3 cut(s)	575, 1388, 2296
PstI	CTGCAG	1 cut(s)	1911
PstNI	CAGNNNCTG	2 cut(s)	488, 1993
PsuI	RGATCY	7 cut(s)	379, 708, 1450, 1981, 1999, 2053, 2273
PsyI	GACNNNGTC	1 cut(s)	2204
RruI	TCGCGA	1 cut(s)	2292
RsaI	GTAC	5 cut(s)	314, 440, 523, 749, 1284
RsaNI	GTAC	5 cut(s)	313, 439, 522, 748, 1283
RseI	CAYNNNNRTG	3 cut(s)	1114, 1503, 1641
SapI	GCTCTTC	1 cut(s)	1128
SatI	GCNGC	8 cut(s)	159, 477, 729, 798, 1615, 1622, 1679, 1910
Sau3AI	GATC	9 cut(s)	379, 708, 1450, 1495, 1876, 1981, 1999, 2053, 2273
Sau96I	GGNCC	3 cut(s)	575, 1388, 2296
ScaI	AGTACT	2 cut(s)	314, 1284
SchI	GAGTC	6 cut(s)	319, 1164, 1172, 1502, 1826, 2174
ScrFI	CCNGG	4 cut(s)	1077, 1675, 1900, 2278
SduI	GDGCHC	3 cut(s)	2069, 2284, 2309
SfaNI	GCATC	9 cut(s)	463, 501, 903, 961, 1106, 1251, 1824, 1902, 2246
SfcI	CTRYAG	1 cut(s)	1907
SinI	GGWCC	2 cut(s)	575, 2296
SmiMI	CAYNNNNRTG	3 cut(s)	1114, 1503, 1641
SmlI	CTYRAG	1 cut(s)	1743
SmoI	CTYRAG	1 cut(s)	1743
SphI	GCATGC	1 cut(s)	1621
SsiI	CCGC	5 cut(s)	47, 108, 836, 1037, 1678
SspI	AATATT	2 cut(s)	853, 1050
SspMI	CTAG	3 cut(s)	1026, 1044, 1682
StyD4I	CCNGG	4 cut(s)	1075, 1673, 1898, 2276
StyI	CCWWGG	2 cut(s)	1405, 1722
TaaI	ACNGT	6 cut(s)	190, 484, 521, 611, 735, 1460
TaiI	ACGT	6 cut(s)	100, 247, 463, 1114, 1292, 1827
TaqI	TCGA	7 cut(s)	92, 702, 1153, 1294, 1714, 1861, 2252
TatI	WGTACW	4 cut(s)	312, 521, 747, 1282
TauI	GCSGC	1 cut(s)	1681
TfiI	GAWTC	7 cut(s)	89, 301, 394, 994, 1030, 1475, 2117
TscAI	CASTG	5 cut(s)	614, 1345, 1561, 2074, 2156
TseFI	GTSAC	2 cut(s)	245, 605
TseI	GCWGC	7 cut(s)	158, 476, 728, 797, 1614, 1621, 1909
Tsp45I	GTSAC	2 cut(s)	245, 605
TspDTI	ATGAA	9 cut(s)	102, 188, 293, 668, 885, 986, 1227, 1301, 1962
TspGWI	ACGGA	1 cut(s)	1515
TspRI	CASTG	5 cut(s)	614, 1345, 1561, 2074, 2156
Tth111I	GACNNNGTC	1 cut(s)	2204
Van91I	CCANNNNNTGG	1 cut(s)	290
VneI	GTGCAC	1 cut(s)	2065
VpaK11BI	GGWCC	2 cut(s)	575, 2296
XapI	RAATTY	4 cut(s)	221, 255, 429, 2092
XceI	RCATGY	2 cut(s)	804, 1621
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1403
XmnI	GAANNNNTTC	2 cut(s)	1322, 1983
XspI	CTAG	3 cut(s)	1026, 1044, 1682
ZrmI	AGTACT	2 cut(s)	314, 1284
Zsp2I	ATGCAT	1 cut(s)	516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.