Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005489

Physical Location & Seq

Genomic Coordinates Forward (+)

58237559 .. 58243815

Gene Structure 6257 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00040520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2304 bp

ATGAGTGTGGTGGGGATTGATATCGGAAATGAAAACTGTGTGATTGCGGTGGTGAAGCAACGGGGGATTGATGTTTTGTTGAATGAGGAATCGAAACGTGAAACCCCGGCTGTGGTTTGCTTCGGCGAGAAGCAAAGGTTTCTGGGGTCTGCTGCTTCTGCCTCTGCAATGATGCACCCAAAATCGACGGTTTCGCAGGTAAAGAGACTTATTGGTAGGAGATTCACGGAGCCTGATGTTCAAAAGGACCTTCGAATGTTGCCTTTTCAAACTTCTGAAGGCCCGGACGGTGGCATTTTGATACATTTGAGTTACTTGGGTGGAATACATACATTTACTCCGGTTCAAATTACGGCGATGCTTTTTGCACATTTGAGAGAGATTATAGAGAAGAATCTGGAAATGCCTGTTTCAGATTGTGTGATCGGTATTCCATCATACTTTACAGACTTGCAGAGACGCGCATATTTGGATGCTGCGATAGTTGCTGGGTTGAAGCCTTTGAGGTTGATGCATGACTGTACTGCAACTGCTCTCAGTTATGGGATTTACAAAACAGATTTCCCCAGTTCTGGTCCGACTTACGTGGCATTTGTTGACATTGGCCATTGTGATACCCAGGTCTCTGTTGCATCTTTTGAGTCTGGGCATATGACGATGCGATCTCACGCTTTTGATAGAAGCTTGGGAGGGAGAGACTTTGATGAGGTCTTGTTTAGTCATTTTGCTGCACAGTTCAAGGAGCAGTATAGAATTGATGTGTACTCTAACGTCAAAGCATGTGTCAGGCTAAGGGCAGCATGTGAGAAGCTGAAAAAGGTTTTGAGTGCAAATGCGGAGGCACCTCTTAATATTGAGTGTTTAATGGATGAGAAAGATGTTAAGGGTTTCATTAAAAGGGAAGAATTTGAAATGCTGGCTTCAAGTTTGTTGGAGAGGATTCGTGTTCCTTGTAGTAAAGCTTTAGCTGATGCAGGTTTAACCGCCGACATGATTCATTCTGTAGAGCTTGTTGGGTCAGGGTCTAGGATCCCAGCTATTGCTAGAGCATTAGTTTCTCTATTCAAGGTAGAACCACGTCGGACACTAAATGCTAGTGAATGTGTAGCACGTGGATGTGCTCTTCAATGTGCAATGCTAAGTCCTGTTTTCCGAGTCAGAGAATATGAGGTTCAAGATGCTATTCCTTTCACTATAGGATTCTGTTCAGATGAAGGTCCAATTGGCACTGGCTCGGATGGCGTCTTGTTTCCAAAAGGCCAACCCATCCCAAGTGCTAAAGTATTAACACTTCAACGAAGCAGTTTATTCCATTTGGAAGCATTGTATGCTGATCCCAGTGAACTGCCTGCTGGTGCATCTCCAAAAATTAGTTGTTTTACGATTGGTCCTTTCCAAGGCTTCCATAGTGACAAGACAAGGGTTAAAGTTAAAATTCAATTAGACCTCCATGGTATTGTCTCTGTTGTATCAGCTAGGATGGTGGAAGAACATGGAGACGATTCAAAAATGGACCCGATGGACACTGATTGTGTAACTGCTTCTGTTTCATCTGAGGCAGCTGAAGACAGGTTTCAAGAGAGTATGCAGTCCAAATCTTCACATGCTACTGGTGATGGTAGACACAATAAAGCCACTAGCAGGCTAGATATACCCATTAGTGAAAATATATACGGTGGAATGACCAAGGCTGAACTTTCAGAAGCTCAAGGAAAAGAACTTCAGTTAGCTCAACAGGACAGAGCTATGGAACAAACCAAAGACAAAAAGAATGCGTTGGAATCTTATGTCTATGAGATGCGGAATAAGCTCTTCAACACATATCGAAGCTTCGCAAGTGACCAAGAGAGGGAGGGCATCTCCAGGAGCCTACAGCAGACAGAGGACTGGCTTTATGATGATGGAGATGACGAAACTGAAAATGCCTATACTTCAAAGCTGGAAGATCTTAAGAAGCTGGTAGATCCAATTGAAAGCCGATATAAAGATGAGGAAGCAAGAGCACAGGCTACTAGAGATCTATTAAAGTGCATTGGTGATTATCGCATGGCTGCGGATTCACTTTCACTCATGGATAAAGAATCGATTGTTACCGAGTGCTTTAAGGTGGAGCAGTGGCTTAGAGAGAAAAACCAACAGCAAAACTCCATGCCAAAGAACATGGACCCAATATTATGGTCGAGTGATATTAAGAGCCGGACTGAGGAGTTGAACTCGAAATTCAAACACATATTTAGATCCAGGGCTTCTCATCGAGATGACCACAAGGGTTCAAACCAGCATGATACTTCTAGACACAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04530 ko04612 map04530 map04612

Pfam Domains

Protein Families

HSP70

Protein Analysis

767

Amino Acids

85.53

Weight (kDa)

5.9

Isoelectric Point (pI)

46.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	3 - 661	1.3e-145	Hsp70 protein
MreB_Mbl	PF06723	116 - 375	2.4e-10	MreB/Mbl protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005489)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11660 AT1G11660 AT1G11660 AT1G11660
fragaria_vesca	FvH4_3g22620 FvH4_3g22620 FvH4_3g22620 FvH4_3g22620 FvH4_3g22620
malus_domestica	MD03G1201700.v1.1 MD03G1201800.v1.1 MD11G1218900.v1.1
prunus_persica	Prupe.4G212200_v2.0.a1
pyrus_communis	pycom03g15300 pycom03g15320 pycom11g19290
rosa_chinensis	RchiOBHm_Chr5g0039371
rosa_laevigata	RLG00000033908
rosa_multiflora	Rmu_co8453851.1_g000001 Rmu_sc0006937.1_g000008 Rmu_sc0007310.1_g000013
rosa_roxburghii	Rroxscaffold_1G00040520 Rroxscaffold_1G00041830
rosa_rugosa	Rorug05G0178400
rosa_samantha	Rh5AG264600 Rh5BG269600 Rh5CG302600 Rh5DG277400
rosa_wichuraiana	Rw5G024820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	187, 965
AccB1I	GGYRCC	1 cut(s)	841
AccI	GTMKAC	1 cut(s)	1621
AccII	CGCG	1 cut(s)	462
AciI	CCGC	5 cut(s)	47, 836, 984, 1801, 2054
AclWI	GGATC	5 cut(s)	1024, 1037, 1328, 1958, 2232
AcoI	YGGCCR	1 cut(s)	604
AcsI	RAATTY	3 cut(s)	905, 1434, 2219
AcuI	CTGAAG	3 cut(s)	297, 1584, 1706
AcvI	CACGTG	1 cut(s)	1112
AcyI	GRCGYC	1 cut(s)	1242
AfaI	GTAC	2 cut(s)	523, 764
AfiI	CCNNNNNNNGG	7 cut(s)	112, 290, 572, 1397, 1641, 1849, 2203
AflII	CTTAAG	1 cut(s)	1949
AjiI	CACGTC	1 cut(s)	1079
AjnI	CCWGG	3 cut(s)	618, 1862, 2240
AjuI	GAANNNNNNNTTGG	2 cut(s)	1206, 1238
Alw21I	GWGCWC	2 cut(s)	1123, 2005
Alw26I	GTCTC	6 cut(s)	199, 451, 628, 690, 1465, 1491
AlwI	GGATC	5 cut(s)	1024, 1037, 1328, 1958, 2232
AoxI	GGCC	3 cut(s)	280, 604, 1258
ApeKI	GCWGC	6 cut(s)	152, 476, 728, 797, 1559, 2051
ApoI	RAATTY	3 cut(s)	905, 1434, 2219
ArsI	GACNNNNNNTTYG	2 cut(s)	768, 800
AspLEI	GCGC	1 cut(s)	464
AspS9I	GGNCC	7 cut(s)	247, 281, 575, 1217, 1388, 1513, 2164
AsuC2I	CCSGG	2 cut(s)	107, 284
AsuHPI	GGTGA	3 cut(s)	64, 1625, 2048
AsuII	TTCGAA	1 cut(s)	253
AvaII	GGWCC	6 cut(s)	247, 575, 1217, 1388, 1513, 2164
BaeI	ACNNNNGTAYC	2 cut(s)	606, 639
BalI	TGGCCA	1 cut(s)	606
BamHI	GGATCC	1 cut(s)	1029
BanI	GGYRCC	1 cut(s)	841
BarI	GAAGNNNNNNTAC	2 cut(s)	1275, 1307
BbrPI	CACGTG	1 cut(s)	1112
BbsI	GAAGAC	1 cut(s)	1572
Bbv12I	GWGCWC	2 cut(s)	1123, 2005
BbvI	GCAGC	6 cut(s)	139, 463, 715, 809, 1571, 2038
BccI	CCATC	7 cut(s)	442, 1232, 1274, 1474, 1513, 1610, 1895
BceAI	ACGGC	1 cut(s)	369
BciT130I	CCWGG	3 cut(s)	620, 1864, 2242
BcnI	CCSGG	2 cut(s)	107, 284
BcoDI	GTCTC	6 cut(s)	199, 451, 628, 690, 1465, 1491
BfaI	CTAG	8 cut(s)	1026, 1044, 1095, 1476, 1638, 1646, 2013, 2292
BfmI	CTRYAG	3 cut(s)	1002, 1194, 1871
BfrI	CTTAAG	1 cut(s)	1949
BfuAI	ACCTGC	2 cut(s)	187, 965
BglII	AGATCT	2 cut(s)	1945, 2017
BisI	GCNGC	6 cut(s)	153, 477, 729, 798, 1560, 2052
BlsI	GCNGC	6 cut(s)	154, 478, 730, 799, 1561, 2053
Bme1390I	CCNGG	5 cut(s)	107, 284, 620, 1864, 2242
Bme18I	GGWCC	6 cut(s)	247, 575, 1217, 1388, 1513, 2164
BmgBI	CACGTC	1 cut(s)	1079
BmgT120I	GGNCC	7 cut(s)	247, 281, 575, 1217, 1388, 1513, 2164
BmiI	GGNNCC	6 cut(s)	231, 843, 1031, 1515, 1868, 2166
BmrFI	CCNGG	5 cut(s)	107, 284, 620, 1864, 2242
BmrI	ACTGGG	2 cut(s)	561, 1332
BmuI	ACTGGG	2 cut(s)	561, 1332
BpiI	GAAGAC	1 cut(s)	1572
BplI	GAGNNNNNCTC	4 cut(s)	1844, 1876, 2198, 2230
BpmI	CTGGAG	1 cut(s)	1846
Bpu10I	CCTNAGC	1 cut(s)	791
Bpu14I	TTCGAA	1 cut(s)	253
BpuEI	CTTGAG	1 cut(s)	1692
BpuMI	CCSGG	2 cut(s)	107, 284
Bsa29I	ATCGAT	1 cut(s)	2084
BsaAI	YACGTR	2 cut(s)	586, 1112
BsaBI	GATNNNNATC	1 cut(s)	20
BsaHI	GRCGYC	1 cut(s)	1242
BsaI	GGTCTC	1 cut(s)	628
BsaJI	CCNNGG	6 cut(s)	105, 618, 1396, 1450, 1686, 2241
BsaWI	WCCGGW	1 cut(s)	340
BsaXI	ACNNNNNCTCC	2 cut(s)	322, 352
Bsc4I	CCNNNNNNNGG	7 cut(s)	112, 290, 572, 1397, 1641, 1849, 2203
Bse1I	ACTGG	5 cut(s)	567, 1234, 1338, 1615, 1892
Bse3DI	GCAATG	2 cut(s)	174, 1140
Bse8I	GATNNNNATC	1 cut(s)	20
BseBI	CCWGG	3 cut(s)	620, 1864, 2242
BseCI	ATCGAT	1 cut(s)	2084
BseDI	CCNNGG	6 cut(s)	105, 618, 1396, 1450, 1686, 2241
BseGI	GGATG	6 cut(s)	478, 874, 1121, 1243, 1266, 1485
BseJI	GATNNNNATC	1 cut(s)	20
BseLI	CCNNNNNNNGG	7 cut(s)	112, 290, 572, 1397, 1641, 1849, 2203
BseMI	GCAATG	2 cut(s)	174, 1140
BseMII	CTCAG	3 cut(s)	550, 1545, 2193
BseNI	ACTGG	5 cut(s)	567, 1234, 1338, 1615, 1892
BseRI	GAGGAG	1 cut(s)	2219
BseXI	GCAGC	6 cut(s)	139, 463, 715, 809, 1571, 2038
BseYI	CCCAGC	2 cut(s)	488, 1033
BsgI	GTGCAG	1 cut(s)	714
Bsh1236I	CGCG	1 cut(s)	462
BshFI	GGCC	3 cut(s)	282, 606, 1260
BshNI	GGYRCC	1 cut(s)	841
BshVI	ATCGAT	1 cut(s)	2084
BsiHKAI	GWGCWC	2 cut(s)	1123, 2005
BsiSI	CCGG	4 cut(s)	107, 284, 341, 2197
BslI	CCNNNNNNNGG	7 cut(s)	112, 290, 572, 1397, 1641, 1849, 2203
BsmAI	GTCTC	6 cut(s)	199, 451, 628, 690, 1465, 1491
BsmBI	CGTCTC	2 cut(s)	451, 1491
BsmI	GAATGC	1 cut(s)	1777
BsnI	GGCC	3 cut(s)	282, 606, 1260
Bso31I	GGTCTC	1 cut(s)	628
Bsp119I	TTCGAA	1 cut(s)	253
Bsp1286I	GDGCHC	2 cut(s)	1123, 2005
Bsp143I	GATC	8 cut(s)	423, 662, 1029, 1333, 1945, 1963, 2017, 2237
Bsp19I	CCATGG	1 cut(s)	1450
BspACI	CCGC	5 cut(s)	47, 836, 984, 1801, 2054
BspANI	GGCC	3 cut(s)	282, 606, 1260
BspCNI	CTCAG	3 cut(s)	549, 1546, 2194
BspDI	ATCGAT	1 cut(s)	2084
BspFNI	CGCG	1 cut(s)	462
BspLI	GGNNCC	6 cut(s)	231, 843, 1031, 1515, 1868, 2166
BspMI	ACCTGC	2 cut(s)	187, 965
BspPI	GGATC	5 cut(s)	1024, 1037, 1328, 1958, 2232
BspQI	GCTCTTC	2 cut(s)	1128, 1817
BspT104I	TTCGAA	1 cut(s)	253
BspT107I	GGYRCC	1 cut(s)	841
BspTI	CTTAAG	1 cut(s)	1949
BspTNI	GGTCTC	1 cut(s)	628
BsrDI	GCAATG	2 cut(s)	174, 1140
BsrI	ACTGG	5 cut(s)	567, 1234, 1338, 1615, 1892
BssECI	CCNNGG	6 cut(s)	105, 618, 1396, 1450, 1686, 2241
BssMI	GATC	8 cut(s)	423, 662, 1029, 1333, 1945, 1963, 2017, 2237
BssNI	GRCGYC	1 cut(s)	1242
BssT1I	CCWWGG	3 cut(s)	1396, 1450, 1686
Bst2UI	CCWGG	3 cut(s)	620, 1864, 2242
Bst4CI	ACNGT	6 cut(s)	38, 190, 290, 521, 735, 1676
Bst6I	CTCTTC	2 cut(s)	1128, 1817
BstACI	GRCGYC	1 cut(s)	1242
BstAFI	CTTAAG	1 cut(s)	1949
BstAPI	GCANNNNNTGC	1 cut(s)	1328
BstBAI	YACGTR	2 cut(s)	586, 1112
BstBI	TTCGAA	1 cut(s)	253
BstC8I	GCNNGC	3 cut(s)	918, 1350, 1643
BstDEI	CTNAG	6 cut(s)	536, 791, 1139, 1554, 2120, 2202
BstDSI	CCRYGG	1 cut(s)	1450
BstENI	CCTNNNNNAGG	1 cut(s)	1395
BstF5I	GGATG	6 cut(s)	478, 874, 1121, 1243, 1266, 1485
BstFNI	CGCG	1 cut(s)	462
BstHHI	GCGC	1 cut(s)	464
BstKTI	GATC	8 cut(s)	426, 665, 1032, 1336, 1948, 1966, 2020, 2240
BstMAI	GTCTC	6 cut(s)	199, 451, 628, 690, 1465, 1491
BstMBI	GATC	8 cut(s)	423, 662, 1029, 1333, 1945, 1963, 2017, 2237
BstMWI	GCNNNNNNNGC	5 cut(s)	158, 485, 1239, 1328, 1807
BstNI	CCWGG	3 cut(s)	620, 1864, 2242
BstNSI	RCATGY	3 cut(s)	783, 804, 1607
BstSCI	CCNGG	5 cut(s)	105, 282, 618, 1862, 2240
BstSFI	CTRYAG	3 cut(s)	1002, 1194, 1871
BstUI	CGCG	1 cut(s)	462
BstV1I	GCAGC	6 cut(s)	139, 463, 715, 809, 1571, 2038
BstV2I	GAAGAC	1 cut(s)	1572
BstX2I	RGATCY	5 cut(s)	1029, 1945, 1963, 2017, 2237
BstXI	CCANNNNNNTGG	1 cut(s)	2175
BstYI	RGATCY	5 cut(s)	1029, 1945, 1963, 2017, 2237
Bsu15I	ATCGAT	1 cut(s)	2084
BsuRI	GGCC	3 cut(s)	282, 606, 1260
BsuTUI	ATCGAT	1 cut(s)	2084
BtgI	CCRYGG	1 cut(s)	1450
BtgZI	GCGATG	1 cut(s)	371
BtrI	CACGTC	1 cut(s)	1079
BtsCI	GGATG	6 cut(s)	478, 874, 1121, 1243, 1266, 1485
BtsI	GCAGTG	1 cut(s)	2120
BtsIMutI	CAGTG	4 cut(s)	1227, 1345, 1524, 2120
BveI	ACCTGC	2 cut(s)	187, 965
Cac8I	GCNNGC	3 cut(s)	918, 1350, 1643
CfoI	GCGC	1 cut(s)	464
Cfr13I	GGNCC	7 cut(s)	247, 281, 575, 1217, 1388, 1513, 2164
ClaI	ATCGAT	1 cut(s)	2084
CseI	GACGC	2 cut(s)	468, 1231
Csp6I	GTAC	2 cut(s)	522, 763
CviQI	GTAC	2 cut(s)	522, 763
DdeI	CTNAG	6 cut(s)	536, 791, 1139, 1554, 2120, 2202
DpnI	GATC	8 cut(s)	425, 664, 1031, 1335, 1947, 1965, 2019, 2239
DpnII	GATC	8 cut(s)	423, 662, 1029, 1333, 1945, 1963, 2017, 2237
EaeI	YGGCCR	1 cut(s)	604
Eam1104I	CTCTTC	2 cut(s)	1128, 1817
EarI	CTCTTC	2 cut(s)	1128, 1817
Eco130I	CCWWGG	3 cut(s)	1396, 1450, 1686
Eco31I	GGTCTC	1 cut(s)	628
Eco32I	GATATC	1 cut(s)	22
Eco47I	GGWCC	6 cut(s)	247, 575, 1217, 1388, 1513, 2164
Eco57I	CTGAAG	3 cut(s)	297, 1584, 1706
Eco72I	CACGTG	1 cut(s)	1112
EcoNI	CCTNNNNNAGG	1 cut(s)	1395
EcoO109I	RGGNCCY	1 cut(s)	247
EcoRII	CCWGG	3 cut(s)	618, 1862, 2240
EcoRV	GATATC	1 cut(s)	22
EcoT14I	CCWWGG	3 cut(s)	1396, 1450, 1686
EcoT22I	ATGCAT	1 cut(s)	516
ErhI	CCWWGG	3 cut(s)	1396, 1450, 1686
Esp3I	CGTCTC	2 cut(s)	451, 1491
FauNDI	CATATG	1 cut(s)	651
FblI	GTMKAC	1 cut(s)	1621
Fnu4HI	GCNGC	6 cut(s)	153, 477, 729, 798, 1560, 2052
FokI	GGATG	6 cut(s)	485, 881, 1128, 1250, 1253, 1492
Fsp4HI	GCNGC	6 cut(s)	153, 477, 729, 798, 1560, 2052
FspBI	CTAG	8 cut(s)	1026, 1044, 1095, 1476, 1638, 1646, 2013, 2292
GlaI	GCGC	1 cut(s)	463
GluI	GCNGC	6 cut(s)	153, 477, 729, 798, 1560, 2052
GsaI	CCCAGC	2 cut(s)	492, 1037
GsuI	CTGGAG	1 cut(s)	1846
HaeIII	GGCC	3 cut(s)	282, 606, 1260
HapII	CCGG	4 cut(s)	107, 284, 341, 2197
HgaI	GACGC	2 cut(s)	468, 1231
HhaI	GCGC	1 cut(s)	464
Hin1I	GRCGYC	1 cut(s)	1242
Hin6I	GCGC	1 cut(s)	462
HinP1I	GCGC	1 cut(s)	462
HincII	GTYRAC	1 cut(s)	598
HindII	GTYRAC	1 cut(s)	598
HindIII	AAGCTT	3 cut(s)	682, 960, 1828
HpaII	CCGG	4 cut(s)	107, 284, 341, 2197
HphI	GGTGA	3 cut(s)	64, 1625, 2048
Hpy166II	GTNNAC	4 cut(s)	598, 763, 1343, 1622
Hpy188III	TCNNGA	5 cut(s)	398, 1175, 1577, 2255, 2292
Hpy8I	GTNNAC	4 cut(s)	598, 763, 1343, 1622
Hpy99I	CGWCG	2 cut(s)	190, 1083
HpyAV	CCTTC	3 cut(s)	260, 272, 1208
HpyCH4III	ACNGT	6 cut(s)	38, 190, 290, 521, 735, 1676
HpyCH4IV	ACGT	5 cut(s)	97, 585, 771, 1078, 1111
HpyF10VI	GCNNNNNNNGC	5 cut(s)	158, 485, 1239, 1328, 1807
HpyF3I	CTNAG	6 cut(s)	536, 791, 1139, 1554, 2120, 2202
HpySE526I	ACGT	5 cut(s)	97, 585, 771, 1078, 1111
Hsp92I	GRCGYC	1 cut(s)	1242
HspAI	GCGC	1 cut(s)	462
Kzo9I	GATC	8 cut(s)	423, 662, 1029, 1333, 1945, 1963, 2017, 2237
LguI	GCTCTTC	2 cut(s)	1128, 1817
LmnI	GCTCC	4 cut(s)	229, 742, 1866, 2110
Lsp1109I	GCAGC	6 cut(s)	139, 463, 715, 809, 1571, 2038
MaeI	CTAG	8 cut(s)	1026, 1044, 1095, 1476, 1638, 1646, 2013, 2292
MaeII	ACGT	5 cut(s)	97, 585, 771, 1078, 1111
MaeIII	GTNAC	5 cut(s)	311, 1409, 1534, 1838, 2089
MalI	GATC	8 cut(s)	425, 664, 1031, 1335, 1947, 1965, 2019, 2239
MboI	GATC	8 cut(s)	423, 662, 1029, 1333, 1945, 1963, 2017, 2237
MboII	GAAGA	8 cut(s)	403, 914, 1115, 1499, 1577, 1590, 1804, 1955
MfeI	CAATTG	2 cut(s)	1221, 1968
MflI	RGATCY	5 cut(s)	1029, 1945, 1963, 2017, 2237
MhlI	GDGCHC	2 cut(s)	1123, 2005
MlsI	TGGCCA	1 cut(s)	606
MluCI	AATT	9 cut(s)	348, 753, 905, 1221, 1368, 1434, 1439, 1968, 2219
MluNI	TGGCCA	1 cut(s)	606
MlyI	GAGTC	2 cut(s)	650, 1164
MmeI	TCCRAC	4 cut(s)	602, 912, 1061, 1758
Mox20I	TGGCCA	1 cut(s)	606
Mph1103I	ATGCAT	1 cut(s)	516
MscI	TGGCCA	1 cut(s)	606
MslI	CAYNNNNRTG	2 cut(s)	1114, 2256
Msp20I	TGGCCA	1 cut(s)	606
MspA1I	CMGCKG	1 cut(s)	1562
MspCI	CTTAAG	1 cut(s)	1949
MspI	CCGG	4 cut(s)	107, 284, 341, 2197
MspR9I	CCNGG	5 cut(s)	107, 284, 620, 1864, 2242
MunI	CAATTG	2 cut(s)	1221, 1968
Mva1269I	GAATGC	1 cut(s)	1777
MvaI	CCWGG	3 cut(s)	620, 1864, 2242
MvnI	CGCG	1 cut(s)	462
MwoI	GCNNNNNNNGC	5 cut(s)	158, 485, 1239, 1328, 1807
NciI	CCSGG	2 cut(s)	107, 284
NcoI	CCATGG	1 cut(s)	1450
NdeI	CATATG	1 cut(s)	651
NdeII	GATC	8 cut(s)	423, 662, 1029, 1333, 1945, 1963, 2017, 2237
NlaIV	GGNNCC	6 cut(s)	231, 843, 1031, 1515, 1868, 2166
NmuCI	GTSAC	2 cut(s)	1409, 1838
NsiI	ATGCAT	1 cut(s)	516
NspI	RCATGY	3 cut(s)	783, 804, 1607
NspV	TTCGAA	1 cut(s)	253
PciSI	GCTCTTC	2 cut(s)	1128, 1817
PctI	GAATGC	1 cut(s)	1777
PfoI	TCCNGGA	1 cut(s)	1862
PkrI	GCNGC	6 cut(s)	154, 478, 730, 799, 1561, 2053
PleI	GAGTC	2 cut(s)	649, 1163
PmaCI	CACGTG	1 cut(s)	1112
PmlI	CACGTG	1 cut(s)	1112
PpsI	GAGTC	2 cut(s)	649, 1163
Ppu21I	YACGTR	2 cut(s)	586, 1112
PpuMI	RGGWCCY	1 cut(s)	247
Psp5II	RGGWCCY	1 cut(s)	247
Psp6I	CCWGG	3 cut(s)	618, 1862, 2240
PspCI	CACGTG	1 cut(s)	1112
PspFI	CCCAGC	2 cut(s)	488, 1033
PspGI	CCWGG	3 cut(s)	618, 1862, 2240
PspN4I	GGNNCC	6 cut(s)	231, 843, 1031, 1515, 1868, 2166
PspPI	GGNCC	7 cut(s)	247, 281, 575, 1217, 1388, 1513, 2164
PspPPI	RGGWCCY	1 cut(s)	247
PsuI	RGATCY	5 cut(s)	1029, 1945, 1963, 2017, 2237
PvuII	CAGCTG	1 cut(s)	1562
RsaI	GTAC	2 cut(s)	523, 764
RsaNI	GTAC	2 cut(s)	522, 763
RseI	CAYNNNNRTG	2 cut(s)	1114, 2256
SapI	GCTCTTC	2 cut(s)	1128, 1817
SatI	GCNGC	6 cut(s)	153, 477, 729, 798, 1560, 2052
Sau3AI	GATC	8 cut(s)	423, 662, 1029, 1333, 1945, 1963, 2017, 2237
Sau96I	GGNCC	7 cut(s)	247, 281, 575, 1217, 1388, 1513, 2164
SchI	GAGTC	2 cut(s)	650, 1164
ScrFI	CCNGG	5 cut(s)	107, 284, 620, 1864, 2242
SduI	GDGCHC	2 cut(s)	1123, 2005
SfcI	CTRYAG	3 cut(s)	1002, 1194, 1871
SfuI	TTCGAA	1 cut(s)	253
SinI	GGWCC	6 cut(s)	247, 575, 1217, 1388, 1513, 2164
SmiMI	CAYNNNNRTG	2 cut(s)	1114, 2256
SmlI	CTYRAG	2 cut(s)	1707, 1949
SmoI	CTYRAG	2 cut(s)	1707, 1949
Sse9I	AATT	9 cut(s)	348, 753, 905, 1221, 1368, 1434, 1439, 1968, 2219
SsiI	CCGC	5 cut(s)	47, 836, 984, 1801, 2054
SspI	AATATT	2 cut(s)	853, 2172
SspMI	CTAG	8 cut(s)	1026, 1044, 1095, 1476, 1638, 1646, 2013, 2292
StyD4I	CCNGG	5 cut(s)	105, 282, 618, 1862, 2240
StyI	CCWWGG	3 cut(s)	1396, 1450, 1686
TaaI	ACNGT	6 cut(s)	38, 190, 290, 521, 735, 1676
TaiI	ACGT	5 cut(s)	100, 588, 774, 1081, 1114
TaqI	TCGA	8 cut(s)	92, 185, 253, 1825, 2084, 2180, 2216, 2254
TasI	AATT	9 cut(s)	348, 753, 905, 1221, 1368, 1434, 1439, 1968, 2219
TatI	WGTACW	2 cut(s)	521, 762
TscAI	CASTG	4 cut(s)	1234, 1345, 1531, 2120
TseFI	GTSAC	2 cut(s)	1409, 1838
TseI	GCWGC	6 cut(s)	152, 476, 728, 797, 1559, 2051
Tsp45I	GTSAC	2 cut(s)	1409, 1838
TspDTI	ATGAA	5 cut(s)	45, 880, 986, 1227, 1539
TspGWI	ACGGA	1 cut(s)	242
TspRI	CASTG	4 cut(s)	1234, 1345, 1531, 2120
Vha464I	CTTAAG	1 cut(s)	1949
VpaK11BI	GGWCC	6 cut(s)	247, 575, 1217, 1388, 1513, 2164
XagI	CCTNNNNNAGG	1 cut(s)	1395
XapI	RAATTY	3 cut(s)	905, 1434, 2219
XbaI	TCTAGA	1 cut(s)	2291
XceI	RCATGY	3 cut(s)	783, 804, 1607
XmiI	GTMKAC	1 cut(s)	1621
XspI	CTAG	8 cut(s)	1026, 1044, 1095, 1476, 1638, 1646, 2013, 2292
Zsp2I	ATGCAT	1 cut(s)	516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.