Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0015833

Physical Location & Seq

Genomic Coordinates Reverse (-)

29660247 .. 29662938

Gene Structure 2692 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1215800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGATAATAACGGTGGACATTTACAACTATGCCCTTCATTTTATTCTTGTATTTACTTTCCCATCCCCATATAACCCTACAGGTGAAAGCAAAGCCACCTCCATTTCCCTGCTCTATATAAACACCGGTTCTCCCTTTCTTTCTCCCCCACTCCTAAACCATCATCTAGTTTTCATCACACCATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATACAGTTTCAAGCCAATCTCTCTCTCTCTCTCTAATTCCAAACTACACACAAACACCATGCCTGAAGCACCGAAGAAATTTCTGAATCCCACGGAGGACTATAACCTCCAAGACAAGAACAACAAGATCCTTCACTTCATCGAAGAAGTCACAAAAACCCCCCACGAGGTTCAAAAACGAGTCCTCGCTGAAATACTCACCCGCAATGCTCACGTCGAGTACTTGCAGCGCCACGGCCTCAACGGTCACACTGACCGTGACACTTTCAAAAAAATCATGCCCGTTATCAAGTACGAAGATATCCAGTCCGATATCAACCGTATCGCCAATGGTGACACCTCCCATATCCTCTGTTCCAAGCCCATTTCTGAGTTTTTGACCAGTTCTGGCACATCCGGAGGGGAAAGAAAACTGATGCCAACAATTGAAGAGGAGCTAGAGAGGAGGTCTTTACTTTACAGTCTGCTGATGCCTGTGATGAGCCAGTATGTCCCCGGACTGGACAAGGGCAAAGGAATGTACTTTTTGTTCATAAAATCCGAGGCCAAAACCCCCGGCGGACTTGTGGCCAGGCCGGTCCTAACCAGCTACTACAAAAGCTCACATTTCAAACAAAGACCCTATGACCCTTACACTAACTACACCAGCCCCAATGAGACCATCCTCTGCCCGGACTCCTACCAAAGCATGTACTCCCAACTCCTCTGCGGCCTCTGCCAGAACAAGGAAGTCCTCCGAGTCGGCGCTGTTTTCGCCTCTGGCTTCATCCGGGCCATCAGGTTTCTTGAGAAGCACTGGACTATTTTGTGCAAGGACATAGCCTCCGGGACTCTTAACCCCCAGATTACAGACCCGTCGGTCCGCGAGGCGGTGATGAAGATCCTTAAGCCTGATCCGAAGCTTGCAAGTTTCATTGAGGGTGAGTGCCGGAGGGACTCTTGGCATGGGATTATTACCAGGCTTTGGCCAAACACAAAGTATGTGGATGTGATTGTGACCGGGACTATGTCCCAGTACATTCCGACACTGGACTATTACAGCAACGGACTGCCTCTGGTTTGCACTATGTATGCCTCTTCGGAGTGCTACTTTGGTGTTAATCTAAACCCTCTGTGCAAACCCAGTGAAGTGTCCTACACTCTTATTCCCACCATGGGATACTTTGAGTTCCTCCCGGTCCAGAGGAACAATGGGATTAATACTAATCCCGTCTCTGTCCGCAAATCACTAAATGAGAAGGAGCATCAAGAACTAGTTGATCTTGCAGATGTGAAGCTTGGACAAGAATATGAGCTTGTTGTCACAACATATGCTGGACTGTACCGCTACAGAGTGGGGGATGTGCTAAGAGTGACCTCATTCAAGAACAAGGCACCGCAGTTCAACTTCATCTGCAGGAAAAATGTTGTCCTGAGCATTGACTCGGACAAGACTGATGAGGTGGAGCTCCAAAACGCAGTGAAAAATGCCGTGAACCATTTAGTCCCGTTCGAGGCCACCGTGACGGAATATACTAGCTATGCGGACACAACAACAATCCCGGGACACTATGTGCTCTTTTGGGAACTTAGCTTCAATGGGACAACTCCAGTCCCACCCTCGGTCTTTGAGGACTGTTGCCTCACCGTCGAAGAATCCCTGAACAGCGTCTACCGGCAAGGCCGGGCCTCCGACAAGTCAATCGGACCACTCGAGATCAAGATTGTCGAGACGGGGACGTTTGACAAGCTCATGGACTATGCCATTAGCTTGGGAGCTTCCATTAACCAATACAAGACCCCTAGGTGTGTGAAATTTGCACCCATTGTGGAGCTCTTGACCACAAGGGTGGTCTCAAACTACCATAGCCCCAAGTGCCCCAAGTGGGTCCCAGGCCACAAGCAATGGTGCAACATTGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006725 GO:0006790 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009058 GO:0009314 GO:0009403 GO:0009404 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009653 GO:0009700 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009826 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010120 GO:0010252 GO:0010279 GO:0016043 GO:0016049 GO:0016874 GO:0016879 GO:0016881 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0032870 GO:0032989 GO:0034641 GO:0040007 GO:0042221 GO:0042430 GO:0042435 GO:0042592 GO:0042762 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0044237 GO:0044249 GO:0044271 GO:0044272 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0046217 GO:0046483 GO:0048518 GO:0048522 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051176 GO:0051716 GO:0052314 GO:0052315 GO:0052317 GO:0052318 GO:0052319 GO:0052320 GO:0052322 GO:0060560 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0071840 GO:1900376 GO:1900378 GO:1901182 GO:1901183 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

GH3

Protein Analysis

710

Amino Acids

80.11

Weight (kDa)

7.53

Isoelectric Point (pI)

40.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH3	PF03321	120 - 428	1.9e-124	GH3 family N-terminal domain
GH3_M	PF23571	455 - 542	1.9e-30	GH3 family central domain
GH3_C	PF23572	558 - 675	2e-38	GH3 family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015833)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27260 AT5G54510
fragaria_vesca	FvH4_3g21460
malus_domestica	MD03G1215800.v1.1 MD11G1230400.v1.1
prunus_persica	Prupe.4G197000_v2.0.a1
pyrus_communis	pycom03g16410 pycom11g20410
rosa_chinensis	RchiOBHm_Chr5g0036891
rosa_laevigata	RLG00000033732
rosa_multiflora	Rmu_sc0007663.1_g000004
rosa_roxburghii	Rroxscaffold_1G00044060
rosa_rugosa	Rorug05G0158600
rosa_samantha	Rh5CG285100
rosa_wichuraiana	Rw5G023080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1085
AccB1I	GGYRCC	1 cut(s)	1600
AccB7I	CCANNNNNTGG	1 cut(s)	1191
AccI	GTMKAC	1 cut(s)	1878
AccII	CGCG	1 cut(s)	1092
AccIII	TCCGGA	1 cut(s)	623
AciI	CCGC	9 cut(s)	430, 786, 936, 1090, 1097, 1447, 1552, 1604, 1751
AclWI	GGATC	3 cut(s)	349, 1102, 1115
AcoI	YGGCCR	2 cut(s)	795, 1193
AcsI	RAATTY	2 cut(s)	305, 2021
AcuI	CTGAAG	1 cut(s)	312
AdeI	CACNNNGTG	1 cut(s)	1780
AfaI	GTAC	6 cut(s)	449, 521, 749, 920, 1244, 1550
AflII	CTTAAG	1 cut(s)	1112
AgeI	ACCGGT	1 cut(s)	125
AgsI	TTSAA	8 cut(s)	238, 401, 496, 656, 838, 1591, 1612, 1804
AhlI	ACTAGT	1 cut(s)	1480
AjiI	CACGTC	1 cut(s)	442
AjnI	CCWGG	3 cut(s)	797, 1184, 2098
AleI	CACNNNNGTG	1 cut(s)	2054
Alw21I	GWGCWC	3 cut(s)	1677, 1785, 2043
Alw26I	GTCTC	4 cut(s)	878, 1444, 1931, 2065
AlwI	GGATC	3 cut(s)	349, 1102, 1115
Ama87I	CYCGRG	2 cut(s)	1768, 1919
Aor13HI	TCCGGA	1 cut(s)	623
ApeKI	GCWGC	1 cut(s)	454
ApoI	RAATTY	2 cut(s)	305, 2021
AseI	ATTAAT	1 cut(s)	1425
AsiGI	ACCGGT	1 cut(s)	125
AspA2I	CCTAGG	1 cut(s)	2009
AspLEI	GCGC	2 cut(s)	459, 974
AspS9I	GGNCC	7 cut(s)	805, 999, 1087, 1405, 1893, 1913, 2095
AsuHPI	GGTGA	6 cut(s)	95, 418, 572, 1111, 1160, 1843
AvaI	CYCGRG	2 cut(s)	1768, 1919
AvaII	GGWCC	5 cut(s)	805, 1087, 1405, 1913, 2095
AvrII	CCTAGG	1 cut(s)	2009
BaeGI	GKGCMC	1 cut(s)	2087
BalI	TGGCCA	2 cut(s)	797, 1195
BanI	GGYRCC	1 cut(s)	1600
BanII	GRGCYC	2 cut(s)	1677, 2043
BauI	CACGAG	1 cut(s)	392
Bbv12I	GWGCWC	3 cut(s)	1677, 1785, 2043
BbvI	GCAGC	1 cut(s)	466
BccI	CCATC	5 cut(s)	70, 168, 190, 896, 1010
BceAI	ACGGC	2 cut(s)	478, 1682
BciT130I	CCWGG	3 cut(s)	799, 1186, 2100
BciVI	GTATCC	1 cut(s)	1379
BcoDI	GTCTC	4 cut(s)	878, 1444, 1931, 2065
BcuI	ACTAGT	1 cut(s)	1480
BfaI	CTAG	5 cut(s)	167, 665, 1481, 1743, 2010
BfmI	CTRYAG	3 cut(s)	78, 1555, 1621
BfoI	RGCGCY	2 cut(s)	460, 975
BfrI	CTTAAG	1 cut(s)	1112
BfuI	GTATCC	1 cut(s)	1379
BisI	GCNGC	2 cut(s)	455, 937
BlnI	CCTAGG	1 cut(s)	2009
BlsI	GCNGC	2 cut(s)	456, 938
BmcAI	AGTACT	1 cut(s)	449
Bme18I	GGWCC	5 cut(s)	805, 1087, 1405, 1913, 2095
BmeT110I	CYCGRG	2 cut(s)	1768, 1919
BmgBI	CACGTC	1 cut(s)	442
BmgT120I	GGNCC	7 cut(s)	805, 999, 1087, 1405, 1893, 1913, 2095
BmiI	GGNNCC	3 cut(s)	1602, 2096, 2097
BmrI	ACTGGG	2 cut(s)	1234, 1344
BmsI	GCATC	3 cut(s)	633, 687, 1480
BmuI	ACTGGG	2 cut(s)	1234, 1344
BpmI	CTGGAG	1 cut(s)	1800
Bpu10I	CCTNAGC	1 cut(s)	1640
BpuEI	CTTGAG	1 cut(s)	1034
BsaBI	GATNNNNATC	1 cut(s)	1106
BsaI	GGTCTC	2 cut(s)	878, 2065
BsaWI	WCCGGW	2 cut(s)	125, 623
BsaXI	ACNNNNNCTCC	4 cut(s)	115, 145, 1034, 1064
Bse118I	RCCGGY	3 cut(s)	125, 802, 1881
Bse1I	ACTGG	9 cut(s)	532, 609, 712, 732, 1028, 1240, 1260, 1350, 1817
Bse3DI	GCAATG	2 cut(s)	439, 2117
Bse8I	GATNNNNATC	1 cut(s)	1106
BseAI	TCCGGA	1 cut(s)	623
BseBI	CCWGG	3 cut(s)	799, 1186, 2100
BseGI	GGATG	6 cut(s)	62, 620, 888, 993, 1219, 1573
BseJI	GATNNNNATC	1 cut(s)	1106
BseMI	GCAATG	2 cut(s)	439, 2117
BseMII	CTCAG	2 cut(s)	588, 1631
BseNI	ACTGG	9 cut(s)	532, 609, 712, 732, 1028, 1240, 1260, 1350, 1817
BseRI	GAGGAG	3 cut(s)	674, 685, 920
BseSI	GKGCMC	1 cut(s)	2087
BseXI	GCAGC	1 cut(s)	466
Bsh1236I	CGCG	1 cut(s)	1092
BshNI	GGYRCC	1 cut(s)	1600
BshTI	ACCGGT	1 cut(s)	125
BsiHKAI	GWGCWC	3 cut(s)	1677, 1785, 2043
BsiHKCI	CYCGRG	2 cut(s)	1768, 1919
BsmAI	GTCTC	4 cut(s)	878, 1444, 1931, 2065
BsmBI	CGTCTC	2 cut(s)	1444, 1931
Bso31I	GGTCTC	2 cut(s)	878, 2065
BsoBI	CYCGRG	2 cut(s)	1768, 1919
Bsp1286I	GDGCHC	4 cut(s)	1677, 1785, 2043, 2087
Bsp13I	TCCGGA	1 cut(s)	623
Bsp143I	GATC	5 cut(s)	354, 1107, 1120, 1486, 1923
Bsp19I	CCATGG	1 cut(s)	1380
BspACI	CCGC	9 cut(s)	430, 786, 936, 1090, 1097, 1447, 1552, 1604, 1751
BspCNI	CTCAG	2 cut(s)	589, 1632
BspEI	TCCGGA	1 cut(s)	623
BspFNI	CGCG	1 cut(s)	1092
BspLI	GGNNCC	3 cut(s)	1602, 2096, 2097
BspMAI	CTGCAG	1 cut(s)	1625
BspPI	GGATC	3 cut(s)	349, 1102, 1115
BspT107I	GGYRCC	1 cut(s)	1600
BspTI	CTTAAG	1 cut(s)	1112
BspTNI	GGTCTC	2 cut(s)	878, 2065
BsrDI	GCAATG	2 cut(s)	439, 2117
BsrFI	RCCGGY	3 cut(s)	125, 802, 1881
BsrI	ACTGG	9 cut(s)	532, 609, 712, 732, 1028, 1240, 1260, 1350, 1817
BssAI	RCCGGY	3 cut(s)	125, 802, 1881
BssMI	GATC	5 cut(s)	354, 1107, 1120, 1486, 1923
BssSI	CACGAG	1 cut(s)	392
BssT1I	CCWWGG	2 cut(s)	1380, 2009
Bst2BI	CACGAG	1 cut(s)	392
Bst2UI	CCWGG	3 cut(s)	799, 1186, 2100
Bst6I	CTCTTC	2 cut(s)	651, 1309
BstAFI	CTTAAG	1 cut(s)	1112
BstC8I	GCNNGC	1 cut(s)	1131
BstDEI	CTNAG	4 cut(s)	597, 1574, 1640, 1796
BstDSI	CCRYGG	3 cut(s)	318, 460, 1380
BstF5I	GGATG	6 cut(s)	62, 620, 888, 993, 1219, 1573
BstFNI	CGCG	1 cut(s)	1092
BstH2I	RGCGCY	2 cut(s)	460, 975
BstHHI	GCGC	2 cut(s)	459, 974
BstKTI	GATC	5 cut(s)	357, 1110, 1123, 1489, 1926
BstMAI	GTCTC	4 cut(s)	878, 1444, 1931, 2065
BstMBI	GATC	5 cut(s)	354, 1107, 1120, 1486, 1923
BstMWI	GCNNNNNNNGC	3 cut(s)	942, 980, 2082
BstNI	CCWGG	3 cut(s)	799, 1186, 2100
BstNSI	RCATGY	1 cut(s)	919
BstSFI	CTRYAG	3 cut(s)	78, 1555, 1621
BstSLI	GKGCMC	1 cut(s)	2087
BstUI	CGCG	1 cut(s)	1092
BstV1I	GCAGC	1 cut(s)	466
BstX2I	RGATCY	2 cut(s)	354, 1107
BstXI	CCANNNNNNTGG	2 cut(s)	1978, 2056
BstYI	RGATCY	2 cut(s)	354, 1107
BsuI	GTATCC	1 cut(s)	1379
BtgI	CCRYGG	3 cut(s)	318, 460, 1380
BtrI	CACGTC	1 cut(s)	442
BtsCI	GGATG	6 cut(s)	62, 620, 888, 993, 1219, 1573
BtsI	GCAGTG	1 cut(s)	1692
BtsIMutI	CAGTG	5 cut(s)	477, 1021, 1253, 1357, 1692
Cac8I	GCNNGC	1 cut(s)	1131
CfoI	GCGC	2 cut(s)	459, 974
Cfr10I	RCCGGY	3 cut(s)	125, 802, 1881
Cfr13I	GGNCC	7 cut(s)	805, 999, 1087, 1405, 1893, 1913, 2095
Cfr9I	CCCGGG	1 cut(s)	1768
CpoI	CGGWCCG	1 cut(s)	1087
CseI	GACGC	1 cut(s)	1864
Csp6I	GTAC	6 cut(s)	448, 520, 748, 919, 1243, 1549
CspAI	ACCGGT	1 cut(s)	125
CspCI	CAANNNNNGTGG	2 cut(s)	1191, 1226
CspI	CGGWCCG	1 cut(s)	1087
CviAII	CATG	6 cut(s)	286, 505, 916, 1172, 1381, 1960
CviQI	GTAC	6 cut(s)	448, 520, 748, 919, 1243, 1549
DdeI	CTNAG	4 cut(s)	597, 1574, 1640, 1796
DpnI	GATC	5 cut(s)	356, 1109, 1122, 1488, 1925
DpnII	GATC	5 cut(s)	354, 1107, 1120, 1486, 1923
DraIII	CACNNNGTG	1 cut(s)	1780
DrdI	GACNNNNNNGTC	1 cut(s)	1085
DseDI	GACNNNNNNGTC	1 cut(s)	1085
EaeI	YGGCCR	2 cut(s)	795, 1193
Eam1104I	CTCTTC	2 cut(s)	651, 1309
EarI	CTCTTC	2 cut(s)	651, 1309
EciI	GGCGGA	1 cut(s)	801
Ecl136II	GAGCTC	2 cut(s)	1675, 2041
Eco130I	CCWWGG	2 cut(s)	1380, 2009
Eco24I	GRGCYC	2 cut(s)	1677, 2043
Eco31I	GGTCTC	2 cut(s)	878, 2065
Eco32I	GATATC	2 cut(s)	529, 541
Eco47I	GGWCC	5 cut(s)	805, 1087, 1405, 1913, 2095
Eco53kI	GAGCTC	2 cut(s)	1675, 2041
Eco57I	CTGAAG	1 cut(s)	312
Eco88I	CYCGRG	2 cut(s)	1768, 1919
EcoICRI	GAGCTC	2 cut(s)	1675, 2041
EcoO109I	RGGNCCY	1 cut(s)	2095
EcoRII	CCWGG	3 cut(s)	797, 1184, 2098
EcoRV	GATATC	2 cut(s)	529, 541
EcoT14I	CCWWGG	2 cut(s)	1380, 2009
EcoT38I	GRGCYC	2 cut(s)	1677, 2043
ErhI	CCWWGG	2 cut(s)	1380, 2009
Esp3I	CGTCTC	2 cut(s)	1444, 1931
FaeI	CATG	6 cut(s)	289, 508, 919, 1175, 1384, 1963
FatI	CATG	6 cut(s)	285, 504, 915, 1171, 1380, 1959
FauI	CCCGC	1 cut(s)	437
FauNDI	CATATG	1 cut(s)	1537
FblI	GTMKAC	1 cut(s)	1878
Fnu4HI	GCNGC	2 cut(s)	455, 937
FokI	GGATG	6 cut(s)	49, 607, 875, 980, 1226, 1580
FriOI	GRGCYC	2 cut(s)	1677, 2043
Fsp4HI	GCNGC	2 cut(s)	455, 937
FspBI	CTAG	5 cut(s)	167, 665, 1481, 1743, 2010
GlaI	GCGC	2 cut(s)	458, 973
GluI	GCNGC	2 cut(s)	455, 937
GsuI	CTGGAG	1 cut(s)	1800
HaeII	RGCGCY	2 cut(s)	460, 975
HgaI	GACGC	1 cut(s)	1864
HhaI	GCGC	2 cut(s)	459, 974
Hin1II	CATG	6 cut(s)	289, 508, 919, 1175, 1384, 1963
Hin6I	GCGC	2 cut(s)	457, 972
HinP1I	GCGC	2 cut(s)	457, 972
HindIII	AAGCTT	2 cut(s)	1127, 1502
HinfI	GANTC	8 cut(s)	313, 408, 902, 966, 1057, 1163, 1649, 1862
HphI	GGTGA	6 cut(s)	95, 418, 572, 1111, 1160, 1843
Hpy166II	GTNNAC	3 cut(s)	16, 1702, 1879
Hpy8I	GTNNAC	3 cut(s)	16, 1702, 1879
Hpy99I	CGWCG	3 cut(s)	446, 1087, 1859
HpyAV	CCTTC	3 cut(s)	44, 368, 1459
HpyCH4IV	ACGT	2 cut(s)	441, 1946
HpyCH4V	TGCA	9 cut(s)	454, 1038, 1133, 1290, 1344, 1493, 1623, 2027, 2118
HpyF10VI	GCNNNNNNNGC	3 cut(s)	942, 980, 2082
HpyF3I	CTNAG	4 cut(s)	597, 1574, 1640, 1796
HpySE526I	ACGT	2 cut(s)	441, 1946
Hsp92II	CATG	6 cut(s)	289, 508, 919, 1175, 1384, 1963
HspAI	GCGC	2 cut(s)	457, 972
KflI	GGGWCCC	1 cut(s)	2095
Kpn2I	TCCGGA	1 cut(s)	623
Kzo9I	GATC	5 cut(s)	354, 1107, 1120, 1486, 1923
LmnI	GCTCC	6 cut(s)	661, 1468, 1672, 1680, 1982, 2038
Lsp1109I	GCAGC	1 cut(s)	466
LweI	GCATC	3 cut(s)	633, 687, 1480
MaeI	CTAG	5 cut(s)	167, 665, 1481, 1743, 2010
MaeII	ACGT	2 cut(s)	441, 1946
MaeIII	GTNAC	8 cut(s)	376, 473, 485, 560, 1222, 1528, 1579, 1729
MalI	GATC	5 cut(s)	356, 1109, 1122, 1488, 1925
MboI	GATC	5 cut(s)	354, 1107, 1120, 1486, 1923
MboII	GAAGA	7 cut(s)	313, 383, 536, 668, 1117, 1296, 1871
MfeI	CAATTG	1 cut(s)	651
MflI	RGATCY	2 cut(s)	354, 1107
MhlI	GDGCHC	4 cut(s)	1677, 1785, 2043, 2087
MlsI	TGGCCA	2 cut(s)	797, 1195
MluCI	AATT	4 cut(s)	262, 305, 651, 2021
MluNI	TGGCCA	2 cut(s)	797, 1195
MlyI	GAGTC	6 cut(s)	417, 896, 975, 1051, 1157, 1643
MmeI	TCCRAC	2 cut(s)	1274, 1923
Mox20I	TGGCCA	2 cut(s)	797, 1195
MroI	TCCGGA	1 cut(s)	623
MscI	TGGCCA	2 cut(s)	797, 1195
MseI	TTAA	5 cut(s)	1062, 1113, 1326, 1425, 1992
MslI	CAYNNNNRTG	1 cut(s)	2054
Msp20I	TGGCCA	2 cut(s)	797, 1195
MspCI	CTTAAG	1 cut(s)	1112
MunI	CAATTG	1 cut(s)	651
MvaI	CCWGG	3 cut(s)	799, 1186, 2100
MvnI	CGCG	1 cut(s)	1092
MwoI	GCNNNNNNNGC	3 cut(s)	942, 980, 2082
NcoI	CCATGG	1 cut(s)	1380
NdeI	CATATG	1 cut(s)	1537
NdeII	GATC	5 cut(s)	354, 1107, 1120, 1486, 1923
NlaIII	CATG	6 cut(s)	289, 508, 919, 1175, 1384, 1963
NlaIV	GGNNCC	3 cut(s)	1602, 2096, 2097
NmuCI	GTSAC	8 cut(s)	376, 473, 485, 560, 1222, 1528, 1579, 1729
NspI	RCATGY	1 cut(s)	919
OliI	CACNNNNGTG	1 cut(s)	2054
PaeR7I	CTCGAG	1 cut(s)	1919
PcsI	WCGNNNNNNNCGW	2 cut(s)	1725, 1917
PfeI	GAWTC	2 cut(s)	313, 1862
PflFI	GACNNNGTC	1 cut(s)	1234
PflMI	CCANNNNNTGG	1 cut(s)	1191
PfoI	TCCNGGA	1 cut(s)	1052
PinAI	ACCGGT	1 cut(s)	125
PkrI	GCNGC	2 cut(s)	456, 938
PleI	GAGTC	6 cut(s)	416, 896, 974, 1051, 1157, 1643
PpsI	GAGTC	6 cut(s)	416, 896, 974, 1051, 1157, 1643
PpuMI	RGGWCCY	1 cut(s)	2095
PshBI	ATTAAT	1 cut(s)	1425
Psp124BI	GAGCTC	2 cut(s)	1677, 2043
Psp5II	RGGWCCY	1 cut(s)	2095
Psp6I	CCWGG	3 cut(s)	797, 1184, 2098
PspGI	CCWGG	3 cut(s)	797, 1184, 2098
PspN4I	GGNNCC	3 cut(s)	1602, 2096, 2097
PspPI	GGNCC	7 cut(s)	805, 999, 1087, 1405, 1893, 1913, 2095
PspPPI	RGGWCCY	1 cut(s)	2095
PsrI	GAACNNNNNNTAC	4 cut(s)	740, 772, 1862, 1894
PstI	CTGCAG	1 cut(s)	1625
PsuI	RGATCY	2 cut(s)	354, 1107
PsyI	GACNNNGTC	1 cut(s)	1234
RsaI	GTAC	6 cut(s)	449, 521, 749, 920, 1244, 1550
RsaNI	GTAC	6 cut(s)	448, 520, 748, 919, 1243, 1549
RseI	CAYNNNNRTG	1 cut(s)	2054
Rsr2I	CGGWCCG	1 cut(s)	1087
RsrII	CGGWCCG	1 cut(s)	1087
SacI	GAGCTC	2 cut(s)	1677, 2043
SaqAI	TTAA	5 cut(s)	1062, 1113, 1326, 1425, 1992
SatI	GCNGC	2 cut(s)	455, 937
Sau3AI	GATC	5 cut(s)	354, 1107, 1120, 1486, 1923
Sau96I	GGNCC	7 cut(s)	805, 999, 1087, 1405, 1893, 1913, 2095
ScaI	AGTACT	1 cut(s)	449
SchI	GAGTC	6 cut(s)	417, 896, 975, 1051, 1157, 1643
SduI	GDGCHC	4 cut(s)	1677, 1785, 2043, 2087
SfaNI	GCATC	3 cut(s)	633, 687, 1480
SfcI	CTRYAG	3 cut(s)	78, 1555, 1621
Sfr274I	CTCGAG	1 cut(s)	1919
SinI	GGWCC	5 cut(s)	805, 1087, 1405, 1913, 2095
SlaI	CTCGAG	1 cut(s)	1919
SmaI	CCCGGG	1 cut(s)	1770
SmiMI	CAYNNNNRTG	1 cut(s)	2054
SmlI	CTYRAG	3 cut(s)	1013, 1112, 1919
SmoI	CTYRAG	3 cut(s)	1013, 1112, 1919
SpeI	ACTAGT	1 cut(s)	1480
Sse9I	AATT	4 cut(s)	262, 305, 651, 2021
SsiI	CCGC	9 cut(s)	430, 786, 936, 1090, 1097, 1447, 1552, 1604, 1751
SspMI	CTAG	5 cut(s)	167, 665, 1481, 1743, 2010
SstI	GAGCTC	2 cut(s)	1677, 2043
StyI	CCWWGG	2 cut(s)	1380, 2009
TaiI	ACGT	2 cut(s)	444, 1949
TaqI	TCGA	6 cut(s)	369, 444, 1719, 1857, 1920, 1935
TaqII	GACCGA	2 cut(s)	1075, 1819
TasI	AATT	4 cut(s)	262, 305, 651, 2021
TatI	WGTACW	4 cut(s)	447, 747, 918, 1242
TauI	GCSGC	1 cut(s)	939
TfiI	GAWTC	2 cut(s)	313, 1862
Tru1I	TTAA	5 cut(s)	1062, 1113, 1326, 1425, 1992
Tru9I	TTAA	5 cut(s)	1062, 1113, 1326, 1425, 1992
TscAI	CASTG	5 cut(s)	484, 1028, 1260, 1357, 1692
TseFI	GTSAC	8 cut(s)	376, 473, 485, 560, 1222, 1528, 1579, 1729
TseI	GCWGC	1 cut(s)	454
Tsp45I	GTSAC	8 cut(s)	376, 473, 485, 560, 1222, 1528, 1579, 1729
TspDTI	ATGAA	8 cut(s)	26, 163, 355, 748, 982, 1118, 1129, 1606
TspGWI	ACGGA	3 cut(s)	335, 1287, 1748
TspMI	CCCGGG	1 cut(s)	1768
TspRI	CASTG	5 cut(s)	484, 1028, 1260, 1357, 1692
Tth111I	GACNNNGTC	1 cut(s)	1234
Van91I	CCANNNNNTGG	1 cut(s)	1191
Vha464I	CTTAAG	1 cut(s)	1112
VpaK11BI	GGWCC	5 cut(s)	805, 1087, 1405, 1913, 2095
VspI	ATTAAT	1 cut(s)	1425
XapI	RAATTY	2 cut(s)	305, 2021
XceI	RCATGY	1 cut(s)	919
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1415
XhoI	CTCGAG	1 cut(s)	1919
XmaI	CCCGGG	1 cut(s)	1768
XmaJI	CCTAGG	1 cut(s)	2009
XmiI	GTMKAC	1 cut(s)	1878
XspI	CTAG	5 cut(s)	167, 665, 1481, 1743, 2010
ZrmI	AGTACT	1 cut(s)	449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.