MD03G1264500.v1.1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0019765
Physical Location & Seq
Forward (+)
35001587 .. 35003331
1745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1264500.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGTAAAAGCCTGTGCCTTTGCACAGAATCATCACACACCTGCCATGAACACGCCTCTCACTGCCAGCGACAGTATGTCTTCAAGAGAAATCGTACCAATTTACATACCAAACCGTACTCTTGAGATGAACCAAGAGATCTACTCACCACATGAAACTGTCACGACAGAAGGACGGGATCGTATAAGGGACTGCATTGCGCGAGCTTTGTCCCTCTTGTCTGAGGAAGATTATGAAAGATATAAGGTTTCTTTCAAGGCATCTGACCCTTCTAAAGTTGCTGCTCAAGTGGAGGCTGCGCTGTTTCAATTTGAACACTCGCCTCCCGATGATGACAAACCATTGGAAGTAAAGCGTCCAGTTCTGTTAGATACTCTTCGGCGCAACAAAGATTTGCGCAGACAAGTTCTTTTTGGAGAGATCACGCCAAAGATGTTGCTCAACATGCCCATAGCCGAACTGCGGCAGAAGTACCCAGCAAACTCACGGCAAATCTTGCTGCCTCCTTCCCGAGAGCGACGTGTTATAAGGCTTTGTATTGCCGGTGGTTTGTCCAAGGTGTCTGAGGAAGTTGATGAACGATATATGGATTCTGTCAATGCATCTGACCCTTCTAAAGTTGGTGCTCAAGTGGAGGCTGCGCTGTTTAAGCACTGGGGTCCCGATGAAACGATGGAACCAGTGGCTTATAGGTATTCACTTCTAATAACTCTTATTAACAACCCTTGGAACAAAGATTTGCGCAGACAAGTTCTTCTTGGGGAGATCACGCCAGAGATGTTGGCCAACATGTCCCTAGCCGAAATGCAACAGTTGAACACGTGGCCGGAGGACGACCCTGTTATCTTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05016 map05016
Pfam Domains
Protein Families
Med26 TFIIS_C TFIIS_M

Protein Analysis

285

Amino Acids

32.4

Weight (kDa)

5.54

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIS_M PF07500 58 - 158 8.4e-11 Transcription factor S-II (TFIIS), central domain
TFIIS_M PF07500 177 - 270 1.3e-11 Transcription factor S-II (TFIIS), central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019765)

Species Orthologous Gene IDs
malus_domestica MD03G1264500.v1.1 MD03G1264600.v1.1 MD04G1039000.v1.1 MD11G1243600.v1.1 MD11G1250500.v1.1 MD11G1285500.v1.1
pyrus_communis pycom11g25300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
AarI CACCTGC 1 cut(s) 49
Acc16I TGCGCA 2 cut(s) 398, 743
Acc36I ACCTGC 1 cut(s) 49
AccII CGCG 1 cut(s) 202
AciI CCGC 1 cut(s) 463
AclWI GGATC 1 cut(s) 186
AcoI YGGCCR 2 cut(s) 783, 824
AcvI CACGTG 1 cut(s) 822
AfaI GTAC 3 cut(s) 96, 118, 473
AfiI CCNNNNNNNGG 1 cut(s) 462
AflIII ACRYGT 3 cut(s) 520, 789, 819
AgsI TTSAA 5 cut(s) 84, 256, 308, 314, 817
AhdI GACNNNNNGTC 1 cut(s) 76
AjiI CACGTC 1 cut(s) 521
AluBI AGCT 1 cut(s) 206
AluI AGCT 1 cut(s) 206
Alw21I GWGCWC 1 cut(s) 628
AlwI GGATC 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 2 cut(s) 783, 824
ApeKI GCWGC 4 cut(s) 281, 296, 499, 638
AspLEI GCGC 6 cut(s) 202, 301, 384, 399, 643, 744
AspS9I GGNCC 1 cut(s) 659
AsuHPI GGTGA 1 cut(s) 138
AvaI CYCGRG 1 cut(s) 510
AvaII GGWCC 1 cut(s) 659
BalI TGGCCA 1 cut(s) 785
BbrPI CACGTG 1 cut(s) 822
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 628
BbvI GCAGC 4 cut(s) 268, 283, 486, 625
BccI CCATC 1 cut(s) 667
BceAI ACGGC 1 cut(s) 503
BfaI CTAG 1 cut(s) 797
BfuAI ACCTGC 1 cut(s) 49
BglII AGATCT 1 cut(s) 138
BisI GCNGC 5 cut(s) 282, 297, 464, 500, 639
BlsI GCNGC 5 cut(s) 283, 298, 465, 501, 640
Bme18I GGWCC 1 cut(s) 659
BmeRI GACNNNNNGTC 1 cut(s) 76
BmeT110I CYCGRG 1 cut(s) 510
BmgBI CACGTC 1 cut(s) 521
BmgT120I GGNCC 1 cut(s) 659
BmiI GGNNCC 3 cut(s) 660, 661, 678
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 2 cut(s) 269, 611
BmuI ACTGGG 1 cut(s) 664
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpuEI CTTGAG 3 cut(s) 143, 270, 612
BsaAI YACGTR 1 cut(s) 822
BsaJI CCNNGG 2 cut(s) 555, 725
Bsc4I CCNNNNNNNGG 1 cut(s) 462
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 3 cut(s) 359, 659, 680
Bse3DI GCAATG 1 cut(s) 195
BseDI CCNNGG 2 cut(s) 555, 725
BseLI CCNNNNNNNGG 1 cut(s) 462
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 2 cut(s) 213, 555
BseNI ACTGG 3 cut(s) 359, 659, 680
BseXI GCAGC 4 cut(s) 268, 283, 486, 625
BseYI CCCAGC 1 cut(s) 475
Bsh1236I CGCG 1 cut(s) 202
BshFI GGCC 2 cut(s) 785, 826
BsiHKAI GWGCWC 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 2 cut(s) 543, 827
BslFI GGGAC 4 cut(s) 196, 203, 645, 778
BslI CCNNNNNNNGG 1 cut(s) 462
BsmFI GGGAC 4 cut(s) 196, 203, 645, 778
BsnI GGCC 2 cut(s) 785, 826
BsoBI CYCGRG 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 628
Bsp143I GATC 4 cut(s) 138, 178, 420, 765
BspACI CCGC 1 cut(s) 463
BspANI GGCC 2 cut(s) 785, 826
BspCNI CTCAG 2 cut(s) 214, 556
BspFNI CGCG 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 660, 661, 678
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 3 cut(s) 359, 659, 680
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 2 cut(s) 555, 725
BssMI GATC 4 cut(s) 138, 178, 420, 765
BssT1I CCWWGG 2 cut(s) 555, 725
Bst4CI ACNGT 4 cut(s) 74, 116, 160, 813
Bst6I CTCTTC 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 496
BstBAI YACGTR 1 cut(s) 822
BstC8I GCNNGC 2 cut(s) 67, 204
BstDEI CTNAG 2 cut(s) 222, 564
BstFNI CGCG 1 cut(s) 202
BstHHI GCGC 6 cut(s) 202, 301, 384, 399, 643, 744
BstKTI GATC 4 cut(s) 141, 181, 423, 768
BstMBI GATC 4 cut(s) 138, 178, 420, 765
BstMWI GCNNNNNNNGC 3 cut(s) 445, 496, 649
BstNSI RCATGY 2 cut(s) 448, 793
BstUI CGCG 1 cut(s) 202
BstV1I GCAGC 4 cut(s) 268, 283, 486, 625
BstV2I GAAGAC 1 cut(s) 72
BstX2I RGATCY 1 cut(s) 138
BstYI RGATCY 1 cut(s) 138
BsuRI GGCC 2 cut(s) 785, 826
BtrI CACGTC 1 cut(s) 521
BtsI GCAGTG 1 cut(s) 60
BtsIMutI CAGTG 3 cut(s) 60, 652, 687
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 2 cut(s) 67, 204
CfoI GCGC 6 cut(s) 202, 301, 384, 399, 643, 744
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 659
CseI GACGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 95, 117, 472
CviAII CATG 4 cut(s) 46, 152, 445, 790
CviQI GTAC 3 cut(s) 95, 117, 472
DdeI CTNAG 2 cut(s) 222, 564
DpnI GATC 4 cut(s) 140, 180, 422, 767
DpnII GATC 4 cut(s) 138, 178, 420, 765
DriI GACNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 2 cut(s) 783, 824
Eam1104I CTCTTC 1 cut(s) 381
Eam1105I GACNNNNNGTC 1 cut(s) 76
EarI CTCTTC 1 cut(s) 381
Eco130I CCWWGG 2 cut(s) 555, 725
Eco47I GGWCC 1 cut(s) 659
Eco72I CACGTG 1 cut(s) 822
Eco88I CYCGRG 1 cut(s) 510
EcoO109I RGGNCCY 1 cut(s) 659
EcoT14I CCWWGG 2 cut(s) 555, 725
EcoT22I ATGCAT 1 cut(s) 604
ErhI CCWWGG 2 cut(s) 555, 725
FaeI CATG 4 cut(s) 49, 155, 448, 793
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FaqI GGGAC 4 cut(s) 196, 203, 645, 778
FatI CATG 4 cut(s) 45, 151, 444, 789
Fnu4HI GCNGC 5 cut(s) 282, 297, 464, 500, 639
Fsp4HI GCNGC 5 cut(s) 282, 297, 464, 500, 639
FspBI CTAG 1 cut(s) 797
FspI TGCGCA 2 cut(s) 398, 743
GlaI GCGC 6 cut(s) 201, 300, 383, 398, 642, 743
GluI GCNGC 5 cut(s) 282, 297, 464, 500, 639
GsaI CCCAGC 1 cut(s) 479
HaeIII GGCC 2 cut(s) 785, 826
HapII CCGG 2 cut(s) 543, 827
HgaI GACGC 1 cut(s) 344
HhaI GCGC 6 cut(s) 202, 301, 384, 399, 643, 744
Hin1II CATG 4 cut(s) 49, 155, 448, 793
Hin6I GCGC 6 cut(s) 200, 299, 382, 397, 641, 742
HinP1I GCGC 6 cut(s) 200, 299, 382, 397, 641, 742
HinfI GANTC 2 cut(s) 28, 590
HpaII CCGG 2 cut(s) 543, 827
HphI GGTGA 1 cut(s) 138
Hpy188I TCNGA 4 cut(s) 223, 265, 565, 607
Hpy188III TCNNGA 7 cut(s) 84, 122, 163, 326, 510, 662, 847
Hpy99I CGWCG 1 cut(s) 522
HpyAV CCTTC 4 cut(s) 164, 279, 516, 621
HpyCH4III ACNGT 4 cut(s) 74, 116, 160, 813
HpyCH4IV ACGT 2 cut(s) 520, 821
HpyCH4V TGCA 4 cut(s) 23, 195, 602, 808
HpyF10VI GCNNNNNNNGC 3 cut(s) 445, 496, 649
HpyF3I CTNAG 2 cut(s) 222, 564
HpySE526I ACGT 2 cut(s) 520, 821
Hsp92II CATG 4 cut(s) 49, 155, 448, 793
HspAI GCGC 6 cut(s) 200, 299, 382, 397, 641, 742
KflI GGGWCCC 1 cut(s) 659
Kzo9I GATC 4 cut(s) 138, 178, 420, 765
Lsp1109I GCAGC 4 cut(s) 268, 283, 486, 625
LweI GCATC 2 cut(s) 269, 611
MaeI CTAG 1 cut(s) 797
MaeII ACGT 2 cut(s) 520, 821
MaeIII GTNAC 1 cut(s) 160
MalI GATC 4 cut(s) 140, 180, 422, 767
MboI GATC 4 cut(s) 138, 178, 420, 765
MboII GAAGA 4 cut(s) 72, 239, 368, 746
MflI RGATCY 1 cut(s) 138
MhlI GDGCHC 1 cut(s) 628
MlsI TGGCCA 1 cut(s) 785
MluCI AATT 2 cut(s) 99, 308
MluNI TGGCCA 1 cut(s) 785
MnlI CCTC 9 cut(s) 66, 217, 224, 286, 333, 513, 559, 628, 823
Mox20I TGGCCA 1 cut(s) 785
Mph1103I ATGCAT 1 cut(s) 604
MscI TGGCCA 1 cut(s) 785
MseI TTAA 2 cut(s) 648, 717
Msp20I TGGCCA 1 cut(s) 785
MspI CCGG 2 cut(s) 543, 827
MvnI CGCG 1 cut(s) 202
MwoI GCNNNNNNNGC 3 cut(s) 445, 496, 649
NdeII GATC 4 cut(s) 138, 178, 420, 765
NlaIII CATG 4 cut(s) 49, 155, 448, 793
NlaIV GGNNCC 3 cut(s) 660, 661, 678
NmuCI GTSAC 1 cut(s) 160
NsbI TGCGCA 2 cut(s) 398, 743
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 2 cut(s) 448, 793
PaqCI CACCTGC 1 cut(s) 49
PciI ACATGT 1 cut(s) 789
PfeI GAWTC 2 cut(s) 28, 590
PkrI GCNGC 5 cut(s) 283, 298, 465, 501, 640
PmaCI CACGTG 1 cut(s) 822
PmlI CACGTG 1 cut(s) 822
Ppu21I YACGTR 1 cut(s) 822
PpuMI RGGWCCY 1 cut(s) 659
PscI ACATGT 1 cut(s) 789
PsiI TTATAA 1 cut(s) 527
Psp5II RGGWCCY 1 cut(s) 659
PspCI CACGTG 1 cut(s) 822
PspFI CCCAGC 1 cut(s) 475
PspN4I GGNNCC 3 cut(s) 660, 661, 678
PspPI GGNCC 1 cut(s) 659
PspPPI RGGWCCY 1 cut(s) 659
PsuI RGATCY 1 cut(s) 138
RsaI GTAC 3 cut(s) 96, 118, 473
RsaNI GTAC 3 cut(s) 95, 117, 472
SaqAI TTAA 2 cut(s) 648, 717
SatI GCNGC 5 cut(s) 282, 297, 464, 500, 639
Sau3AI GATC 4 cut(s) 138, 178, 420, 765
Sau96I GGNCC 1 cut(s) 659
SduI GDGCHC 1 cut(s) 628
SetI ASST 7 cut(s) 43, 208, 249, 523, 561, 695, 824
SfaNI GCATC 2 cut(s) 269, 611
SinI GGWCC 1 cut(s) 659
SmlI CTYRAG 3 cut(s) 122, 285, 627
SmoI CTYRAG 3 cut(s) 122, 285, 627
Sse9I AATT 2 cut(s) 99, 308
SsiI CCGC 1 cut(s) 463
SspMI CTAG 1 cut(s) 797
StyI CCWWGG 2 cut(s) 555, 725
TaaI ACNGT 4 cut(s) 74, 116, 160, 813
TaiI ACGT 2 cut(s) 523, 824
TasI AATT 2 cut(s) 99, 308
TauI GCSGC 1 cut(s) 466
TfiI GAWTC 2 cut(s) 28, 590
Tru1I TTAA 2 cut(s) 648, 717
Tru9I TTAA 2 cut(s) 648, 717
TscAI CASTG 3 cut(s) 67, 659, 687
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 4 cut(s) 281, 296, 499, 638
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 6 cut(s) 62, 143, 168, 249, 591, 681
TspRI CASTG 3 cut(s) 67, 659, 687
VpaK11BI GGWCC 1 cut(s) 659
XceI RCATGY 2 cut(s) 448, 793
XspI CTAG 1 cut(s) 797
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.