MD11G1285500.v1.1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0019765
Physical Location & Seq
Reverse (-)
40395376 .. 40397125
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1285500.v1.1.491

Sequence Viewer

Length: 660 bp
ATGGAGCGTGATCGATCTGCAATCTCAGAGAGAGAGATAAAGAATATGGCGTCGGCGTCATCGTCATCGTCATCGTCGGCTTCGGCGTCAGCTTCAGGGTCAAAGTTAAGGGCAGACGCGAAACCCTACATACGGGGGTCGAACATCGGGTTTTCGTCGGAGGTACCTCTACCTGCGACTCTGTGCGCTTTCCCGGTTTGCGACGGTTCGAGGATGCCAGTACCGGTGTTCAAACCTTTTCCGGATTCTCCTATAGAAGAAGTAGCTCCTGGAGGATATACGCCAGGGCTTCTCCCCCCAGATGAAAACTCAGCGCGGGCTTTTGTAAAGAATTTCATTTCGGTCCAGTTGTCCAAGGTATCTGAGGAAGTTGATGAAAGTTTAGTTGATTATGTCAATGCATTGGACCCTTCTAAAGTTGCTGCTCAAGTGGAGGCTGTGCTATTTGAACGGTGGGGTTTCTTTGATCTGTCGAAACCAGTGAGTAGAAACTATTCGGTGGTTTTAAGTCGCCTGAGCAACGATGGTATCAAAGATTTCCGCAGACACATTCTTCTTGGAGAGATCTCCCCGCAGACATTGTTCAACATGTCCTTAGATGAACTGTGGCAGAAGTACCTGCCCCCAAACAGAATGGGCCCTATGGTTTGGGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05016 map05016
Pfam Domains
Protein Families
Med26 TFIIS_C TFIIS_M

Protein Analysis

220

Amino Acids

24.07

Weight (kDa)

5.19

Isoelectric Point (pI)

61.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIS_M PF07500 103 - 204 3.2e-08 Transcription factor S-II (TFIIS), central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019765)

Species Orthologous Gene IDs
malus_domestica MD03G1264500.v1.1 MD03G1264600.v1.1 MD04G1039000.v1.1 MD11G1243600.v1.1 MD11G1250500.v1.1 MD11G1285500.v1.1
pyrus_communis pycom11g25300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 181, 627
Acc65I GGTACC 1 cut(s) 163
AccB1I GGYRCC 1 cut(s) 163
AccII CGCG 2 cut(s) 119, 316
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 3 cut(s) 316, 541, 572
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 3 cut(s) 50, 56, 86
AfaI GTAC 3 cut(s) 165, 222, 617
AfiI CCNNNNNNNGG 1 cut(s) 132
AflIII ACRYGT 1 cut(s) 588
AgeI ACCGGT 1 cut(s) 223
AgsI TTSAA 3 cut(s) 232, 449, 586
AjnI CCWGG 2 cut(s) 268, 283
AluBI AGCT 2 cut(s) 92, 266
AluI AGCT 2 cut(s) 92, 266
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 1 cut(s) 637
ApaI GGGCCC 1 cut(s) 641
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 1 cut(s) 331
AsiGI ACCGGT 1 cut(s) 223
Asp700I GAANNNNTTC 1 cut(s) 493
Asp718I GGTACC 1 cut(s) 163
AspLEI GCGC 2 cut(s) 188, 316
AspS9I GGNCC 4 cut(s) 343, 406, 637, 638
AsuC2I CCSGG 1 cut(s) 194
AvaII GGWCC 2 cut(s) 343, 406
BaeGI GKGCMC 1 cut(s) 641
BaeI ACNNNNGTAYC 2 cut(s) 511, 544
BanI GGYRCC 1 cut(s) 163
BanII GRGCYC 1 cut(s) 641
BbvI GCAGC 1 cut(s) 409
BccI CCATC 1 cut(s) 518
BciT130I CCWGG 2 cut(s) 270, 285
BcnI CCSGG 1 cut(s) 194
BfmI CTRYAG 1 cut(s) 252
BfuAI ACCTGC 2 cut(s) 181, 627
BglII AGATCT 1 cut(s) 564
BisI GCNGC 1 cut(s) 423
BlsI GCNGC 1 cut(s) 424
Bme1390I CCNGG 3 cut(s) 194, 270, 285
Bme18I GGWCC 2 cut(s) 343, 406
BmgT120I GGNCC 4 cut(s) 343, 406, 637, 638
BmiI GGNNCC 3 cut(s) 165, 408, 639
BmrFI CCNGG 3 cut(s) 194, 270, 285
BmsI GCATC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 411
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 13
BsaHI GRCGYC 3 cut(s) 50, 56, 86
BsaJI CCNNGG 2 cut(s) 284, 354
BsaWI WCCGGW 2 cut(s) 223, 241
Bsc4I CCNNNNNNNGG 1 cut(s) 132
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 3 cut(s) 218, 346, 479
BseAI TCCGGA 1 cut(s) 241
BseBI CCWGG 2 cut(s) 270, 285
BseCI ATCGAT 1 cut(s) 13
BseDI CCNNGG 2 cut(s) 284, 354
BseGI GGATG 1 cut(s) 219
BseLI CCNNNNNNNGG 1 cut(s) 132
BseMII CTCAG 4 cut(s) 39, 324, 354, 506
BseNI ACTGG 3 cut(s) 218, 346, 479
BseSI GKGCMC 1 cut(s) 641
BseXI GCAGC 1 cut(s) 409
Bsh1236I CGCG 2 cut(s) 119, 316
BshFI GGCC 1 cut(s) 639
BshNI GGYRCC 1 cut(s) 163
BshTI ACCGGT 1 cut(s) 223
BshVI ATCGAT 1 cut(s) 13
BsiSI CCGG 3 cut(s) 194, 224, 242
BslI CCNNNNNNNGG 1 cut(s) 132
BsnI GGCC 1 cut(s) 639
Bsp120I GGGCCC 1 cut(s) 637
Bsp1286I GDGCHC 1 cut(s) 641
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 4 cut(s) 10, 14, 466, 564
BspACI CCGC 3 cut(s) 316, 541, 572
BspANI GGCC 1 cut(s) 639
BspCNI CTCAG 4 cut(s) 38, 323, 355, 507
BspDI ATCGAT 1 cut(s) 13
BspEI TCCGGA 1 cut(s) 241
BspFNI CGCG 2 cut(s) 119, 316
BspLI GGNNCC 3 cut(s) 165, 408, 639
BspMI ACCTGC 2 cut(s) 181, 627
BspT107I GGYRCC 1 cut(s) 163
BsrFI RCCGGY 1 cut(s) 223
BsrI ACTGG 3 cut(s) 218, 346, 479
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 2 cut(s) 284, 354
BssMI GATC 4 cut(s) 10, 14, 466, 564
BssNI GRCGYC 3 cut(s) 50, 56, 86
BssT1I CCWWGG 1 cut(s) 354
Bst2UI CCWGG 2 cut(s) 270, 285
Bst4CI ACNGT 3 cut(s) 206, 453, 606
BstACI GRCGYC 3 cut(s) 50, 56, 86
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 5 cut(s) 25, 310, 363, 515, 595
BstF5I GGATG 1 cut(s) 219
BstFNI CGCG 2 cut(s) 119, 316
BstHHI GCGC 2 cut(s) 188, 316
BstKTI GATC 4 cut(s) 13, 17, 469, 567
BstMBI GATC 4 cut(s) 10, 14, 466, 564
BstNI CCWGG 2 cut(s) 270, 285
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 3 cut(s) 192, 268, 283
BstSFI CTRYAG 1 cut(s) 252
BstSLI GKGCMC 1 cut(s) 641
BstUI CGCG 2 cut(s) 119, 316
BstV1I GCAGC 1 cut(s) 409
BstX2I RGATCY 1 cut(s) 564
BstYI RGATCY 1 cut(s) 564
Bsu15I ATCGAT 1 cut(s) 13
BsuRI GGCC 1 cut(s) 639
BsuTUI ATCGAT 1 cut(s) 13
BtsCI GGATG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 486
BveI ACCTGC 2 cut(s) 181, 627
Cac8I GCNNGC 1 cut(s) 318
CfoI GCGC 2 cut(s) 188, 316
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 4 cut(s) 343, 406, 637, 638
ClaI ATCGAT 1 cut(s) 13
CseI GACGC 4 cut(s) 39, 45, 75, 125
Csp6I GTAC 3 cut(s) 164, 221, 616
CspAI ACCGGT 1 cut(s) 223
CviAII CATG 1 cut(s) 589
CviJI RGCY 7 cut(s) 80, 92, 266, 289, 320, 437, 639
CviKI_1 RGCY 7 cut(s) 80, 92, 266, 289, 320, 437, 639
CviQI GTAC 3 cut(s) 164, 221, 616
DdeI CTNAG 5 cut(s) 25, 310, 363, 515, 595
DpnI GATC 4 cut(s) 12, 16, 468, 566
DpnII GATC 4 cut(s) 10, 14, 466, 564
Eco130I CCWWGG 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 641
Eco47I GGWCC 2 cut(s) 343, 406
Eco57I CTGAAG 1 cut(s) 78
EcoO109I RGGNCCY 1 cut(s) 638
EcoRII CCWGG 2 cut(s) 268, 283
EcoT14I CCWWGG 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 641
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 1 cut(s) 592
FaiI YATR 7 cut(s) 47, 131, 254, 279, 393, 590, 644
FatI CATG 1 cut(s) 588
FauI CCCGC 2 cut(s) 309, 579
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 1 cut(s) 226
FriOI GRGCYC 1 cut(s) 641
Fsp4HI GCNGC 1 cut(s) 423
GlaI GCGC 2 cut(s) 187, 315
GluI GCNGC 1 cut(s) 423
GsuI CTGGAG 1 cut(s) 291
HaeIII GGCC 1 cut(s) 639
HapII CCGG 3 cut(s) 194, 224, 242
HgaI GACGC 4 cut(s) 39, 45, 75, 125
HhaI GCGC 2 cut(s) 188, 316
Hin1I GRCGYC 3 cut(s) 50, 56, 86
Hin1II CATG 1 cut(s) 592
Hin6I GCGC 2 cut(s) 186, 314
HinP1I GCGC 2 cut(s) 186, 314
HinfI GANTC 2 cut(s) 178, 245
HpaII CCGG 3 cut(s) 194, 224, 242
Hpy188I TCNGA 3 cut(s) 28, 160, 364
Hpy188III TCNNGA 1 cut(s) 242
Hpy99I CGWCG 4 cut(s) 55, 79, 160, 206
HpyAV CCTTC 1 cut(s) 420
HpyCH4III ACNGT 3 cut(s) 206, 453, 606
HpyCH4V TGCA 2 cut(s) 20, 401
HpyF3I CTNAG 5 cut(s) 25, 310, 363, 515, 595
Hsp92I GRCGYC 3 cut(s) 50, 56, 86
Hsp92II CATG 1 cut(s) 592
HspAI GCGC 2 cut(s) 186, 314
Kpn2I TCCGGA 1 cut(s) 241
KpnI GGTACC 1 cut(s) 167
Kzo9I GATC 4 cut(s) 10, 14, 466, 564
LmnI GCTCC 2 cut(s) 4, 271
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 1 cut(s) 204
MalI GATC 4 cut(s) 12, 16, 468, 566
MboI GATC 4 cut(s) 10, 14, 466, 564
MboII GAAGA 2 cut(s) 269, 545
MflI RGATCY 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 641
MluCI AATT 1 cut(s) 331
MlyI GAGTC 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 138
MnlI CCTC 6 cut(s) 154, 177, 204, 266, 358, 427
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 493
MseI TTAA 2 cut(s) 107, 506
MspI CCGG 3 cut(s) 194, 224, 242
MspR9I CCNGG 3 cut(s) 194, 270, 285
MvaI CCWGG 2 cut(s) 270, 285
MvnI CGCG 2 cut(s) 119, 316
NciI CCSGG 1 cut(s) 194
NdeII GATC 4 cut(s) 10, 14, 466, 564
NlaIII CATG 1 cut(s) 592
NlaIV GGNNCC 3 cut(s) 165, 408, 639
NsiI ATGCAT 1 cut(s) 403
NspI RCATGY 1 cut(s) 592
PciI ACATGT 1 cut(s) 588
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 83
PdmI GAANNNNTTC 1 cut(s) 493
PfeI GAWTC 1 cut(s) 245
PfoI TCCNGGA 1 cut(s) 268
PinAI ACCGGT 1 cut(s) 223
PkrI GCNGC 1 cut(s) 424
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PscI ACATGT 1 cut(s) 588
Psp6I CCWGG 2 cut(s) 268, 283
PspGI CCWGG 2 cut(s) 268, 283
PspN4I GGNNCC 3 cut(s) 165, 408, 639
PspOMI GGGCCC 1 cut(s) 637
PspPI GGNCC 4 cut(s) 343, 406, 637, 638
PsuI RGATCY 1 cut(s) 564
RsaI GTAC 3 cut(s) 165, 222, 617
RsaNI GTAC 3 cut(s) 164, 221, 616
SaqAI TTAA 2 cut(s) 107, 506
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 4 cut(s) 10, 14, 466, 564
Sau96I GGNCC 4 cut(s) 343, 406, 637, 638
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 3 cut(s) 194, 270, 285
SduI GDGCHC 1 cut(s) 641
SetI ASST 8 cut(s) 94, 165, 169, 175, 238, 268, 360, 621
SfaNI GCATC 1 cut(s) 204
SfcI CTRYAG 1 cut(s) 252
SinI GGWCC 2 cut(s) 343, 406
SmlI CTYRAG 1 cut(s) 426
SmoI CTYRAG 1 cut(s) 426
Sse9I AATT 1 cut(s) 331
SsiI CCGC 3 cut(s) 316, 541, 572
StyD4I CCNGG 3 cut(s) 192, 268, 283
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 3 cut(s) 206, 453, 606
TaqI TCGA 4 cut(s) 13, 140, 209, 473
TaqII GACCGA 1 cut(s) 331
TasI AATT 1 cut(s) 331
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 107, 506
Tru9I TTAA 2 cut(s) 107, 506
TscAI CASTG 1 cut(s) 486
TseI GCWGC 1 cut(s) 422
TspDTI ATGAA 4 cut(s) 318, 325, 390, 615
TspRI CASTG 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 343, 406
XapI RAATTY 1 cut(s) 331
XceI RCATGY 1 cut(s) 592
XmnI GAANNNNTTC 1 cut(s) 493
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.