MD03G1264600.v1.1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0019765
Physical Location & Seq
Forward (+)
35009793 .. 35011157
1365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1264600.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGCGTCGGGGTCAAGGGCAGACGCCAAACCCTGCATCCCTTTTCCGATACCTAAAAACCGTTCAATCGCCCAGGTAACCCCGGGGCTGATTGATATCCCCATAGAAGGAGATGTAACTCCTGCACAATTTACGCCAGCGCCTGTCTCCCTAGAAACTGTCACAACAGAAGGACGGGATCGTATAAGGGACTGCATTGCGCGAGCTTTGTCCCTCTTGTCTGAGGAAGATTATGAAAGATATAAGGTTTCTTTTAAGGCATCTGACTCTTCTAAAGTTGCTGCTCAAGTGGAGGCTGCGCTGTTTCAATTTGAACACTCGCCTCCCGATGATGACAAACCATTGGAAGTAAAGCGTCCAGTTCTGTTAGATACTCTTCGGCGCAACAAAGATTTGCGCAGACAAGTTCTTTTTGGAGAGATCACGCCAGAGATGTTGCTCAACATGCCCATAGCCGAACTGCGGCAGAAGTACCCAGCAAACTCACGGCAAATCTTGCTGCCTCCTTCCCGAGAGCGACGTGTTATAAGGCTTTGTATTGCCGGTGGTTTGTCCAAGGTGTCTGAGGAAGTTGATGAACGATATATGGATTCTGTCAATGCATCTGACCCTTCTAAAGTTGCTGCTCAAGTGGAGGCTGCGCTGTTTAAGCACTGGGGTCCCGATGAAACGATGGAACCAGTGGCTTATAGGTATTCACTTCTAATAACTCTTATTAACAACCCTTGGAACAAAGATTTGCGCAGACAAGTTCTTCTTGGGGAGATCACGCCAGAGATGTTGGCCAACATGTCCCTAGCCGAAATGCAACAGTTGAACACGTGGCCGGAGGACGACCCTGTTATCTTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05016 map05016
Pfam Domains
Protein Families
Med26 TFIIS_C TFIIS_M

Protein Analysis

285

Amino Acids

31.95

Weight (kDa)

5.15

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIS_M PF07500 58 - 158 1.3e-10 Transcription factor S-II (TFIIS), central domain
TFIIS_M PF07500 177 - 270 1.3e-12 Transcription factor S-II (TFIIS), central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019765)

Species Orthologous Gene IDs
malus_domestica MD03G1264500.v1.1 MD03G1264600.v1.1 MD04G1039000.v1.1 MD11G1243600.v1.1 MD11G1250500.v1.1 MD11G1285500.v1.1
pyrus_communis pycom11g25300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
Acc16I TGCGCA 2 cut(s) 398, 743
AccII CGCG 1 cut(s) 202
AciI CCGC 1 cut(s) 463
AclWI GGATC 1 cut(s) 186
AcoI YGGCCR 2 cut(s) 783, 824
AcvI CACGTG 1 cut(s) 822
AcyI GRCGYC 2 cut(s) 5, 24
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 2 cut(s) 107, 462
AflIII ACRYGT 3 cut(s) 520, 789, 819
AgsI TTSAA 4 cut(s) 66, 308, 314, 817
AjiI CACGTC 1 cut(s) 521
AjnI CCWGG 1 cut(s) 72
AluBI AGCT 1 cut(s) 206
AluI AGCT 1 cut(s) 206
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 1 cut(s) 186
AlwNI CAGNNNCTG 1 cut(s) 143
Ama87I CYCGRG 2 cut(s) 82, 510
AoxI GGCC 2 cut(s) 783, 824
ApeKI GCWGC 5 cut(s) 281, 296, 499, 623, 638
AspLEI GCGC 7 cut(s) 142, 202, 301, 384, 399, 643, 744
AspS9I GGNCC 1 cut(s) 659
AsuC2I CCSGG 2 cut(s) 83, 84
AvaI CYCGRG 2 cut(s) 82, 510
AvaII GGWCC 1 cut(s) 659
BalI TGGCCA 1 cut(s) 785
BbrPI CACGTG 1 cut(s) 822
BbvI GCAGC 5 cut(s) 268, 283, 486, 610, 625
BccI CCATC 1 cut(s) 667
BceAI ACGGC 1 cut(s) 503
BciT130I CCWGG 1 cut(s) 74
BcnI CCSGG 2 cut(s) 83, 84
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 2 cut(s) 152, 797
BfoI RGCGCY 1 cut(s) 143
BisI GCNGC 6 cut(s) 282, 297, 464, 500, 624, 639
BlsI GCNGC 6 cut(s) 283, 298, 465, 501, 625, 640
Bme1390I CCNGG 3 cut(s) 74, 83, 84
Bme18I GGWCC 1 cut(s) 659
BmeT110I CYCGRG 2 cut(s) 82, 510
BmgBI CACGTC 1 cut(s) 521
BmgT120I GGNCC 1 cut(s) 659
BmiI GGNNCC 3 cut(s) 660, 661, 678
BmrFI CCNGG 3 cut(s) 74, 83, 84
BmrI ACTGGG 1 cut(s) 664
BmsI GCATC 3 cut(s) 45, 269, 611
BmuI ACTGGG 1 cut(s) 664
BpuEI CTTGAG 2 cut(s) 270, 612
BpuMI CCSGG 2 cut(s) 83, 84
BsaAI YACGTR 1 cut(s) 822
BsaBI GATNNNNATC 1 cut(s) 95
BsaHI GRCGYC 2 cut(s) 5, 24
BsaJI CCNNGG 6 cut(s) 72, 81, 82, 83, 555, 725
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 462
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 3 cut(s) 359, 659, 680
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 95
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 6 cut(s) 72, 81, 82, 83, 555, 725
BseGI GGATG 1 cut(s) 36
BseJI GATNNNNATC 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 107, 462
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 2 cut(s) 213, 555
BseNI ACTGG 3 cut(s) 359, 659, 680
BseXI GCAGC 5 cut(s) 268, 283, 486, 610, 625
BseYI CCCAGC 1 cut(s) 475
BsgI GTGCAG 1 cut(s) 108
Bsh1236I CGCG 1 cut(s) 202
BshFI GGCC 2 cut(s) 785, 826
BsiHKCI CYCGRG 2 cut(s) 82, 510
BsiSI CCGG 3 cut(s) 83, 543, 827
BslFI GGGAC 4 cut(s) 196, 203, 645, 778
BslI CCNNNNNNNGG 2 cut(s) 107, 462
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 4 cut(s) 196, 203, 645, 778
BsnI GGCC 2 cut(s) 785, 826
BsoBI CYCGRG 2 cut(s) 82, 510
Bsp143I GATC 3 cut(s) 178, 420, 765
BspACI CCGC 1 cut(s) 463
BspANI GGCC 2 cut(s) 785, 826
BspCNI CTCAG 2 cut(s) 214, 556
BspFNI CGCG 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 660, 661, 678
BspPI GGATC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 542
BsrI ACTGG 3 cut(s) 359, 659, 680
BssAI RCCGGY 1 cut(s) 542
BssECI CCNNGG 6 cut(s) 72, 81, 82, 83, 555, 725
BssMI GATC 3 cut(s) 178, 420, 765
BssNI GRCGYC 2 cut(s) 5, 24
BssT1I CCWWGG 2 cut(s) 555, 725
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 3 cut(s) 62, 160, 813
Bst6I CTCTTC 2 cut(s) 274, 381
BstACI GRCGYC 2 cut(s) 5, 24
BstAPI GCANNNNNTGC 1 cut(s) 496
BstBAI YACGTR 1 cut(s) 822
BstC8I GCNNGC 2 cut(s) 138, 204
BstDEI CTNAG 2 cut(s) 222, 564
BstEII GGTNACC 1 cut(s) 76
BstF5I GGATG 1 cut(s) 36
BstFNI CGCG 1 cut(s) 202
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 7 cut(s) 142, 202, 301, 384, 399, 643, 744
BstKTI GATC 3 cut(s) 181, 423, 768
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 3 cut(s) 178, 420, 765
BstMWI GCNNNNNNNGC 3 cut(s) 445, 496, 649
BstNI CCWGG 1 cut(s) 74
BstNSI RCATGY 2 cut(s) 448, 793
BstPI GGTNACC 1 cut(s) 76
BstSCI CCNGG 3 cut(s) 72, 81, 82
BstUI CGCG 1 cut(s) 202
BstV1I GCAGC 5 cut(s) 268, 283, 486, 610, 625
BsuRI GGCC 2 cut(s) 785, 826
BtrI CACGTC 1 cut(s) 521
BtsCI GGATG 1 cut(s) 36
BtsIMutI CAGTG 2 cut(s) 652, 687
Cac8I GCNNGC 2 cut(s) 138, 204
CaiI CAGNNNCTG 1 cut(s) 143
CfoI GCGC 7 cut(s) 142, 202, 301, 384, 399, 643, 744
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 1 cut(s) 659
Cfr9I CCCGGG 1 cut(s) 82
CseI GACGC 2 cut(s) 32, 344
Csp6I GTAC 1 cut(s) 472
CviAII CATG 2 cut(s) 445, 790
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 2 cut(s) 222, 564
DpnI GATC 3 cut(s) 180, 422, 767
DpnII GATC 3 cut(s) 178, 420, 765
EaeI YGGCCR 2 cut(s) 783, 824
Eam1104I CTCTTC 2 cut(s) 274, 381
EarI CTCTTC 2 cut(s) 274, 381
Eco130I CCWWGG 2 cut(s) 555, 725
Eco32I GATATC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 659
Eco72I CACGTG 1 cut(s) 822
Eco88I CYCGRG 2 cut(s) 82, 510
Eco91I GGTNACC 1 cut(s) 76
EcoO109I RGGNCCY 1 cut(s) 659
EcoO65I GGTNACC 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 72
EcoRV GATATC 1 cut(s) 97
EcoT14I CCWWGG 2 cut(s) 555, 725
EcoT22I ATGCAT 1 cut(s) 604
ErhI CCWWGG 2 cut(s) 555, 725
FaeI CATG 2 cut(s) 448, 793
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FaqI GGGAC 4 cut(s) 196, 203, 645, 778
FatI CATG 2 cut(s) 444, 789
Fnu4HI GCNGC 6 cut(s) 282, 297, 464, 500, 624, 639
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 6 cut(s) 282, 297, 464, 500, 624, 639
FspBI CTAG 2 cut(s) 152, 797
FspI TGCGCA 2 cut(s) 398, 743
GlaI GCGC 7 cut(s) 141, 201, 300, 383, 398, 642, 743
GluI GCNGC 6 cut(s) 282, 297, 464, 500, 624, 639
GsaI CCCAGC 1 cut(s) 479
HaeII RGCGCY 1 cut(s) 143
HaeIII GGCC 2 cut(s) 785, 826
HapII CCGG 3 cut(s) 83, 543, 827
HgaI GACGC 2 cut(s) 32, 344
HhaI GCGC 7 cut(s) 142, 202, 301, 384, 399, 643, 744
Hin1I GRCGYC 2 cut(s) 5, 24
Hin1II CATG 2 cut(s) 448, 793
Hin6I GCGC 7 cut(s) 140, 200, 299, 382, 397, 641, 742
HinP1I GCGC 7 cut(s) 140, 200, 299, 382, 397, 641, 742
HinfI GANTC 2 cut(s) 266, 590
HpaII CCGG 3 cut(s) 83, 543, 827
Hpy188I TCNGA 5 cut(s) 48, 223, 265, 565, 607
Hpy188III TCNNGA 4 cut(s) 326, 510, 662, 847
Hpy99I CGWCG 2 cut(s) 10, 522
HpyAV CCTTC 4 cut(s) 101, 164, 516, 621
HpyCH4III ACNGT 3 cut(s) 62, 160, 813
HpyCH4IV ACGT 2 cut(s) 520, 821
HpyCH4V TGCA 5 cut(s) 36, 125, 195, 602, 808
HpyF10VI GCNNNNNNNGC 3 cut(s) 445, 496, 649
HpyF3I CTNAG 2 cut(s) 222, 564
HpySE526I ACGT 2 cut(s) 520, 821
Hsp92I GRCGYC 2 cut(s) 5, 24
Hsp92II CATG 2 cut(s) 448, 793
HspAI GCGC 7 cut(s) 140, 200, 299, 382, 397, 641, 742
KflI GGGWCCC 1 cut(s) 659
Kzo9I GATC 3 cut(s) 178, 420, 765
Lsp1109I GCAGC 5 cut(s) 268, 283, 486, 610, 625
LweI GCATC 3 cut(s) 45, 269, 611
MaeI CTAG 2 cut(s) 152, 797
MaeII ACGT 2 cut(s) 520, 821
MaeIII GTNAC 3 cut(s) 76, 115, 160
MalI GATC 3 cut(s) 180, 422, 767
MboI GATC 3 cut(s) 178, 420, 765
MboII GAAGA 4 cut(s) 239, 261, 368, 746
MlsI TGGCCA 1 cut(s) 785
MluCI AATT 2 cut(s) 128, 308
MluNI TGGCCA 1 cut(s) 785
MlyI GAGTC 1 cut(s) 260
MnlI CCTC 8 cut(s) 217, 224, 286, 333, 513, 559, 628, 823
Mox20I TGGCCA 1 cut(s) 785
Mph1103I ATGCAT 1 cut(s) 604
MscI TGGCCA 1 cut(s) 785
MseI TTAA 3 cut(s) 255, 648, 717
Msp20I TGGCCA 1 cut(s) 785
MspI CCGG 3 cut(s) 83, 543, 827
MspR9I CCNGG 3 cut(s) 74, 83, 84
MvaI CCWGG 1 cut(s) 74
MvnI CGCG 1 cut(s) 202
MwoI GCNNNNNNNGC 3 cut(s) 445, 496, 649
NciI CCSGG 2 cut(s) 83, 84
NdeII GATC 3 cut(s) 178, 420, 765
NlaIII CATG 2 cut(s) 448, 793
NlaIV GGNNCC 3 cut(s) 660, 661, 678
NmuCI GTSAC 1 cut(s) 160
NsbI TGCGCA 2 cut(s) 398, 743
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 2 cut(s) 448, 793
PciI ACATGT 1 cut(s) 789
PfeI GAWTC 1 cut(s) 590
PkrI GCNGC 6 cut(s) 283, 298, 465, 501, 625, 640
PleI GAGTC 1 cut(s) 260
PmaCI CACGTG 1 cut(s) 822
PmlI CACGTG 1 cut(s) 822
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 822
PpuMI RGGWCCY 1 cut(s) 659
PscI ACATGT 1 cut(s) 789
PsiI TTATAA 1 cut(s) 527
Psp5II RGGWCCY 1 cut(s) 659
Psp6I CCWGG 1 cut(s) 72
PspCI CACGTG 1 cut(s) 822
PspEI GGTNACC 1 cut(s) 76
PspFI CCCAGC 1 cut(s) 475
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 3 cut(s) 660, 661, 678
PspPI GGNCC 1 cut(s) 659
PspPPI RGGWCCY 1 cut(s) 659
PstNI CAGNNNCTG 1 cut(s) 143
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
SaqAI TTAA 3 cut(s) 255, 648, 717
SatI GCNGC 6 cut(s) 282, 297, 464, 500, 624, 639
Sau3AI GATC 3 cut(s) 178, 420, 765
Sau96I GGNCC 1 cut(s) 659
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 3 cut(s) 74, 83, 84
SetI ASST 8 cut(s) 55, 78, 208, 249, 523, 561, 695, 824
SfaNI GCATC 3 cut(s) 45, 269, 611
SinI GGWCC 1 cut(s) 659
SmaI CCCGGG 1 cut(s) 84
SmlI CTYRAG 2 cut(s) 285, 627
SmoI CTYRAG 2 cut(s) 285, 627
Sse9I AATT 2 cut(s) 128, 308
SsiI CCGC 1 cut(s) 463
SspMI CTAG 2 cut(s) 152, 797
StyD4I CCNGG 3 cut(s) 72, 81, 82
StyI CCWWGG 2 cut(s) 555, 725
TaaI ACNGT 3 cut(s) 62, 160, 813
TaiI ACGT 2 cut(s) 523, 824
TasI AATT 2 cut(s) 128, 308
TauI GCSGC 1 cut(s) 466
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 3 cut(s) 255, 648, 717
Tru9I TTAA 3 cut(s) 255, 648, 717
TscAI CASTG 2 cut(s) 659, 687
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 5 cut(s) 281, 296, 499, 623, 638
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 3 cut(s) 249, 591, 681
TspMI CCCGGG 1 cut(s) 82
TspRI CASTG 2 cut(s) 659, 687
VpaK11BI GGWCC 1 cut(s) 659
XceI RCATGY 2 cut(s) 448, 793
XmaI CCCGGG 1 cut(s) 82
XspI CTAG 2 cut(s) 152, 797
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.