MD11G1250500.v1.1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0019765
Physical Location & Seq
Reverse (-)
36257614 .. 36258708
1095 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1250500.v1.1.491

Sequence Viewer

Length: 435 bp
ATGCAGAAGGAGCACCTTCCACTTGGGTTGGAACCTCGTGGTACTTCAGTATCGGAAGTTTTACCCCAAGAAGAAGAAAAGGAACGGTCTTTTGCAAGGAGGTTCATTACGTTCTGTTTGTCCAAGGTATCTAAGGAAACTGATGAAAAATATATGGATTCTGTCAATGCATGTGACCCTTCTAAAGTTGCTGCTCAACTGGAGGCCGTGCTGTTTGAAAAATGGGGTTTCAATGATCAGACGCTGCTATCGACCAAAAAATATTCAGCTGTGTTTTCTTGCCTCCATAATTCTGCGATCAAAGACTTCCGTAGGCAAGTTCTTCTGGGAGAGATCTCCCCAAAACAGTTGGTCAGCATGTCTCCAGATGAGGTTTATGAGATGTGCAAGCCGCGTAGTGATGATACTCAGAGATGCATTACTCGTTTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05016 map05016
Pfam Domains
Protein Families
Med26 TFIIS_C TFIIS_M

Protein Analysis

145

Amino Acids

16.57

Weight (kDa)

6.31

Isoelectric Point (pI)

56.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIS_M PF07500 26 - 125 5.4e-12 Transcription factor S-II (TFIIS), central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019765)

Species Orthologous Gene IDs
malus_domestica MD03G1264500.v1.1 MD03G1264600.v1.1 MD04G1039000.v1.1 MD11G1243600.v1.1 MD11G1250500.v1.1 MD11G1285500.v1.1
pyrus_communis pycom11g25300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 394
AciI CCGC 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 1 cut(s) 43
AgsI TTSAA 2 cut(s) 218, 232
AluBI AGCT 1 cut(s) 269
AluI AGCT 1 cut(s) 269
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 366
AlwNI CAGNNNCTG 1 cut(s) 244
AoxI GGCC 1 cut(s) 204
ApeKI GCWGC 2 cut(s) 191, 244
BaeI ACNNNNGTAYC 4 cut(s) 33, 33, 66, 66
BauI CACGAG 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 2 cut(s) 178, 231
BceAI ACGGC 1 cut(s) 191
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 1 cut(s) 366
BglII AGATCT 1 cut(s) 333
BisI GCNGC 3 cut(s) 192, 245, 392
BlsI GCNGC 3 cut(s) 193, 246, 393
BmiI GGNNCC 1 cut(s) 33
BmsI GCATC 1 cut(s) 404
BpmI CTGGAG 2 cut(s) 221, 348
BsaJI CCNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 422
BseNI ACTGG 1 cut(s) 204
BseXI GCAGC 2 cut(s) 178, 231
Bsh1236I CGCG 1 cut(s) 394
BshFI GGCC 1 cut(s) 206
BsiHKAI GWGCWC 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 366
BsnI GGCC 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 15
Bsp143I GATC 3 cut(s) 235, 297, 333
BspACI CCGC 1 cut(s) 392
BspANI GGCC 1 cut(s) 206
BspCNI CTCAG 1 cut(s) 421
BspFNI CGCG 1 cut(s) 394
BspLI GGNNCC 1 cut(s) 33
BsrI ACTGG 1 cut(s) 204
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 3 cut(s) 235, 297, 333
BssSI CACGAG 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 123
Bst2BI CACGAG 1 cut(s) 36
Bst4CI ACNGT 2 cut(s) 87, 348
BstC8I GCNNGC 1 cut(s) 389
BstDEI CTNAG 2 cut(s) 132, 408
BstFNI CGCG 1 cut(s) 394
BstKTI GATC 3 cut(s) 238, 300, 336
BstMAI GTCTC 1 cut(s) 366
BstMBI GATC 3 cut(s) 235, 297, 333
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNSI RCATGY 2 cut(s) 174, 361
BstUI CGCG 1 cut(s) 394
BstV1I GCAGC 2 cut(s) 178, 231
BstX2I RGATCY 1 cut(s) 333
BstYI RGATCY 1 cut(s) 333
BsuRI GGCC 1 cut(s) 206
Cac8I GCNNGC 1 cut(s) 389
CaiI CAGNNNCTG 1 cut(s) 244
CseI GACGC 1 cut(s) 250
Csp6I GTAC 1 cut(s) 42
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 2 cut(s) 171, 358
CviJI RGCY 3 cut(s) 206, 269, 391
CviKI_1 RGCY 3 cut(s) 206, 269, 391
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 2 cut(s) 132, 408
DpnI GATC 3 cut(s) 237, 299, 335
DpnII GATC 3 cut(s) 235, 297, 333
Eco130I CCWWGG 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 30
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 2 cut(s) 172, 419
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 2 cut(s) 174, 361
FaiI YATR 6 cut(s) 153, 155, 172, 288, 359, 378
FatI CATG 2 cut(s) 170, 357
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 3 cut(s) 192, 245, 392
Fsp4HI GCNGC 3 cut(s) 192, 245, 392
GluI GCNGC 3 cut(s) 192, 245, 392
GsuI CTGGAG 2 cut(s) 221, 348
HaeIII GGCC 1 cut(s) 206
HgaI GACGC 1 cut(s) 250
Hin1II CATG 2 cut(s) 174, 361
HinfI GANTC 1 cut(s) 158
Hpy188I TCNGA 3 cut(s) 55, 240, 411
Hpy188III TCNNGA 1 cut(s) 365
HpyAV CCTTC 2 cut(s) 26, 189
HpyCH4III ACNGT 2 cut(s) 87, 348
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 5 cut(s) 4, 95, 170, 387, 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 2 cut(s) 132, 408
HpySE526I ACGT 1 cut(s) 110
Hsp92II CATG 2 cut(s) 174, 361
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 3 cut(s) 235, 297, 333
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 3 cut(s) 185, 311, 378
Lsp1109I GCAGC 2 cut(s) 178, 231
LweI GCATC 1 cut(s) 404
MaeII ACGT 1 cut(s) 110
MaeIII GTNAC 1 cut(s) 173
MalI GATC 3 cut(s) 237, 299, 335
MboI GATC 3 cut(s) 235, 297, 333
MboII GAAGA 3 cut(s) 83, 86, 314
MflI RGATCY 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 15
MluCI AATT 1 cut(s) 289
MmeI TCCRAC 1 cut(s) 9
MnlI CCTC 5 cut(s) 45, 93, 196, 293, 364
Mph1103I ATGCAT 2 cut(s) 172, 419
MspA1I CMGCKG 1 cut(s) 269
MvnI CGCG 1 cut(s) 394
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 3 cut(s) 235, 297, 333
NlaIII CATG 2 cut(s) 174, 361
NlaIV GGNNCC 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 173
NsiI ATGCAT 2 cut(s) 172, 419
NspI RCATGY 2 cut(s) 174, 361
PcsI WCGNNNNNNNCGW 1 cut(s) 248
PfeI GAWTC 1 cut(s) 158
PkrI GCNGC 3 cut(s) 193, 246, 393
PspN4I GGNNCC 1 cut(s) 33
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 333
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
SatI GCNGC 3 cut(s) 192, 245, 392
Sau3AI GATC 3 cut(s) 235, 297, 333
SduI GDGCHC 1 cut(s) 15
SetI ASST 7 cut(s) 18, 37, 104, 113, 129, 271, 375
SfaNI GCATC 1 cut(s) 404
Sse9I AATT 1 cut(s) 289
SsiI CCGC 1 cut(s) 392
SspI AATATT 1 cut(s) 263
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 2 cut(s) 87, 348
TaiI ACGT 1 cut(s) 113
TaqI TCGA 1 cut(s) 251
TasI AATT 1 cut(s) 289
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 1 cut(s) 158
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 2 cut(s) 191, 244
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 2 cut(s) 94, 159
TspGWI ACGGA 1 cut(s) 299
XceI RCATGY 2 cut(s) 174, 361
Zsp2I ATGCAT 2 cut(s) 172, 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.