MD11G1243600.v1.1

transcription, DNA-templated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0019765
Physical Location & Seq
Forward (+)
35372522 .. 35376191
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1243600.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGGCCATGCACATGGAGGCTGAAGTACCTGGAATCGGCGCCGTTGGGTGTTATGCGGAGAAGCAGAGCTCGCTTTGTGGCAAGAGGAAGAGGCAGCAGAAGCCGCTGGAGGAGGAAGACCGTCAACCTAGGTCAGTTACTCCAGGAGCGTTGCGAGTTTTACCCCAAGATGAAGAAAAGGAATGGGCTTTTACAAGGAATTTCATTTCGTTCCTTTTGTCCAAGGTATCTAAGGAAACTGATGCACAATATATGGATTCTGTCAATTCATGTGACCCTTCTAAAGTTGCTGCTCAACTGGAGTCCGTGCTGTTTGAAAACTGGGGTTTCAATGATCAGTCGCTGGAATCGACTGAAAAGTATTCTACTCTATTTTATTTCCTCCATGCTCCTGCGAGAAAAGATTTCCGCAGACAGGTTCTTCTCGGCGAGATCTCACCAACACAGTTGGTGCACATGTCTTTGGATGAACCGCATTCGAAGTACCCCAATCATATGGACGACCTTTGCGAGAATTGCTATGAAGTTTGGTTTGAGAAACTTCGAAACAGCTTAATTGAGTGTGGTATGATGAAACGTGTAACTGTGGAGCCGCTGGGGACTAAGCAGCGGCAGCAGCTGGACCCTTCACCTCGGTTGGACCCTCCTGGTACTGCAGTATGGGGAGTTTTACGCCAAGTTTTACCCCAAGATATTGAAAAGGAACGGGCTTATGAAAGGAATTTCATTTCGTTCCTTTTGTCCAAGGTATCTAAGGAAACTGATGCACGATATATGGATTCTGTCAATGCATGTGACCCTTCTAAAGTTGCTGCTCAACTGGAGTCCGTGCTGTTTGAAAACTGGGGTTTCAAAGGTCAGTCGCCGGAATCGAATGAAAAGTATTCATCTGTGTTTTCTTGCCTCCAGAATCCTGCGAACAAAGACTTGCGTAGGCAAGTTCTTCTGGGAGAGATCTCCTCAAAACAGTTGGTCAACATGTCTCGAGATGAGGTTTATGATTACTTGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05016 map05016
Pfam Domains
Protein Families
Med26 TFIIS_C TFIIS_M

Protein Analysis

338

Amino Acids

38.69

Weight (kDa)

5.39

Isoelectric Point (pI)

65.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIS_M PF07500 67 - 157 1.2e-10 Transcription factor S-II (TFIIS), central domain
TFIIS_M PF07500 240 - 332 1.8e-14 Transcription factor S-II (TFIIS), central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019765)

Species Orthologous Gene IDs
malus_domestica MD03G1264500.v1.1 MD03G1264600.v1.1 MD04G1039000.v1.1 MD11G1243600.v1.1 MD11G1250500.v1.1 MD11G1285500.v1.1
pyrus_communis pycom11g25300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 38
AciI CCGC 6 cut(s) 56, 104, 409, 473, 593, 610
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 199, 721
AcuI CTGAAG 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 3 cut(s) 27, 485, 652
AfiI CCNNNNNNNGG 2 cut(s) 35, 415
AflIII ACRYGT 3 cut(s) 456, 577, 978
AgsI TTSAA 5 cut(s) 317, 331, 698, 839, 853
AjnI CCWGG 3 cut(s) 28, 142, 646
AluBI AGCT 3 cut(s) 69, 552, 619
AluI AGCT 3 cut(s) 69, 552, 619
Alw21I GWGCWC 2 cut(s) 71, 456
Alw26I GTCTC 1 cut(s) 987
Alw44I GTGCAC 1 cut(s) 452
AlwNI CAGNNNCTG 2 cut(s) 343, 619
Ama87I CYCGRG 1 cut(s) 984
AoxI GGCC 1 cut(s) 3
ApaLI GTGCAC 1 cut(s) 452
ApeKI GCWGC 6 cut(s) 94, 290, 607, 613, 616, 812
ApoI RAATTY 2 cut(s) 199, 721
AspA2I CCTAGG 1 cut(s) 128
AspLEI GCGC 1 cut(s) 41
AspS9I GGNCC 2 cut(s) 622, 640
AsuHPI GGTGA 2 cut(s) 429, 621
AsuII TTCGAA 2 cut(s) 479, 544
AvaI CYCGRG 1 cut(s) 984
AvaII GGWCC 2 cut(s) 622, 640
AvrII CCTAGG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 456
BaeI ACNNNNGTAYC 2 cut(s) 642, 675
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 2 cut(s) 71, 456
BbvI GCAGC 6 cut(s) 106, 277, 619, 625, 628, 799
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 3 cut(s) 30, 144, 648
BclI TGATCA 1 cut(s) 334
BcoDI GTCTC 1 cut(s) 987
BfaI CTAG 1 cut(s) 129
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 42
BglII AGATCT 2 cut(s) 432, 954
BisI GCNGC 9 cut(s) 95, 104, 291, 593, 608, 611, 614, 617, 813
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 9 cut(s) 96, 105, 292, 594, 609, 612, 615, 618, 814
Bme1390I CCNGG 3 cut(s) 30, 144, 648
Bme18I GGWCC 2 cut(s) 622, 640
BmeT110I CYCGRG 1 cut(s) 984
BmgT120I GGNCC 2 cut(s) 622, 640
BmiI GGNNCC 4 cut(s) 40, 591, 624, 642
BmrFI CCNGG 3 cut(s) 30, 144, 648
BmrI ACTGGG 2 cut(s) 331, 853
BmsI GCATC 2 cut(s) 232, 754
BmuI ACTGGG 2 cut(s) 331, 853
BpiI GAAGAC 1 cut(s) 123
BplI GAGNNNNNCTC 2 cut(s) 944, 976
BpmI CTGGAG 5 cut(s) 126, 128, 320, 842, 890
Bpu14I TTCGAA 2 cut(s) 479, 544
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 128, 222, 632, 744
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 415
Bse1I ACTGG 4 cut(s) 303, 326, 825, 848
BseBI CCWGG 3 cut(s) 30, 144, 648
BseDI CCNNGG 4 cut(s) 128, 222, 632, 744
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 2 cut(s) 35, 415
BseNI ACTGG 4 cut(s) 303, 326, 825, 848
BseRI GAGGAG 2 cut(s) 125, 949
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 6 cut(s) 106, 277, 619, 625, 628, 799
BseYI CCCAGC 1 cut(s) 595
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 71, 456
BsiHKCI CYCGRG 1 cut(s) 984
BsiSI CCGG 1 cut(s) 866
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 2 cut(s) 35, 415
BsmAI GTCTC 1 cut(s) 987
BsmFI GGGAC 1 cut(s) 613
BsmI GAATGC 1 cut(s) 475
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 984
Bsp119I TTCGAA 2 cut(s) 479, 544
Bsp1286I GDGCHC 2 cut(s) 71, 456
Bsp143I GATC 3 cut(s) 334, 432, 954
BspACI CCGC 6 cut(s) 56, 104, 409, 473, 593, 610
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 4 cut(s) 40, 591, 624, 642
BspMAI CTGCAG 1 cut(s) 658
BspT104I TTCGAA 2 cut(s) 479, 544
BspT107I GGYRCC 1 cut(s) 38
BsrI ACTGG 4 cut(s) 303, 326, 825, 848
BssECI CCNNGG 4 cut(s) 128, 222, 632, 744
BssMI GATC 3 cut(s) 334, 432, 954
BssNI GRCGYC 1 cut(s) 39
BssT1I CCWWGG 3 cut(s) 128, 222, 744
Bst2UI CCWGG 3 cut(s) 30, 144, 648
Bst4CI ACNGT 4 cut(s) 122, 447, 586, 969
Bst6I CTCTTC 1 cut(s) 83
BstACI GRCGYC 1 cut(s) 39
BstBI TTCGAA 2 cut(s) 479, 544
BstC8I GCNNGC 1 cut(s) 71
BstDEI CTNAG 3 cut(s) 231, 603, 753
BstF5I GGATG 1 cut(s) 472
BstH2I RGCGCY 1 cut(s) 42
BstHHI GCGC 1 cut(s) 41
BstKTI GATC 3 cut(s) 337, 435, 957
BstMAI GTCTC 1 cut(s) 987
BstMBI GATC 3 cut(s) 334, 432, 954
BstMWI GCNNNNNNNGC 6 cut(s) 70, 100, 103, 516, 613, 616
BstNI CCWGG 3 cut(s) 30, 144, 648
BstNSI RCATGY 3 cut(s) 460, 795, 982
BstSCI CCNGG 3 cut(s) 28, 142, 646
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 1 cut(s) 456
BstV1I GCAGC 6 cut(s) 106, 277, 619, 625, 628, 799
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 2 cut(s) 432, 954
BstXI CCANNNNNNTGG 2 cut(s) 13, 496
BstYI RGATCY 2 cut(s) 432, 954
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 472
Cac8I GCNNGC 1 cut(s) 71
CaiI CAGNNNCTG 2 cut(s) 343, 619
CfoI GCGC 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 622, 640
Csp6I GTAC 3 cut(s) 26, 484, 651
CviAII CATG 7 cut(s) 7, 13, 270, 386, 457, 792, 979
CviJI RGCY 9 cut(s) 5, 20, 69, 103, 188, 552, 592, 619, 710
CviKI_1 RGCY 9 cut(s) 5, 20, 69, 103, 188, 552, 592, 619, 710
CviQI GTAC 3 cut(s) 26, 484, 651
DdeI CTNAG 3 cut(s) 231, 603, 753
DinI GGCGCC 1 cut(s) 40
DpnI GATC 3 cut(s) 336, 434, 956
DpnII GATC 3 cut(s) 334, 432, 954
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 69
Eco130I CCWWGG 3 cut(s) 128, 222, 744
Eco24I GRGCYC 1 cut(s) 71
Eco47I GGWCC 2 cut(s) 622, 640
Eco53kI GAGCTC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 984
EcoICRI GAGCTC 1 cut(s) 69
EcoRII CCWGG 3 cut(s) 28, 142, 646
EcoT14I CCWWGG 3 cut(s) 128, 222, 744
EcoT22I ATGCAT 1 cut(s) 793
EcoT38I GRGCYC 1 cut(s) 71
EgeI GGCGCC 1 cut(s) 40
EheI GGCGCC 1 cut(s) 40
ErhI CCWWGG 3 cut(s) 128, 222, 744
FaeI CATG 7 cut(s) 10, 16, 273, 389, 460, 795, 982
FaqI GGGAC 1 cut(s) 613
FatI CATG 7 cut(s) 6, 12, 269, 385, 456, 791, 978
FauNDI CATATG 1 cut(s) 495
FbaI TGATCA 1 cut(s) 334
Fnu4HI GCNGC 9 cut(s) 95, 104, 291, 593, 608, 611, 614, 617, 813
FokI GGATG 1 cut(s) 479
FriOI GRGCYC 1 cut(s) 71
Fsp4HI GCNGC 9 cut(s) 95, 104, 291, 593, 608, 611, 614, 617, 813
FspBI CTAG 1 cut(s) 129
GlaI GCGC 1 cut(s) 40
GluI GCNGC 9 cut(s) 95, 104, 291, 593, 608, 611, 614, 617, 813
GsaI CCCAGC 1 cut(s) 599
GsuI CTGGAG 5 cut(s) 126, 128, 320, 842, 890
HaeII RGCGCY 1 cut(s) 42
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 866
HhaI GCGC 1 cut(s) 41
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 7 cut(s) 10, 16, 273, 389, 460, 795, 982
Hin6I GCGC 1 cut(s) 39
HinP1I GCGC 1 cut(s) 39
HincII GTYRAC 2 cut(s) 125, 976
HindII GTYRAC 2 cut(s) 125, 976
HinfI GANTC 8 cut(s) 33, 257, 302, 347, 779, 824, 869, 910
HpaII CCGG 1 cut(s) 866
HphI GGTGA 2 cut(s) 429, 621
Hpy166II GTNNAC 3 cut(s) 125, 454, 976
Hpy188III TCNNGA 3 cut(s) 907, 984, 986
Hpy8I GTNNAC 3 cut(s) 125, 454, 976
HpyAV CCTTC 3 cut(s) 288, 636, 810
HpyCH4III ACNGT 4 cut(s) 122, 447, 586, 969
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 6 cut(s) 10, 245, 454, 656, 767, 791
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 100, 103, 516, 613, 616
HpyF3I CTNAG 3 cut(s) 231, 603, 753
HpySE526I ACGT 1 cut(s) 577
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 7 cut(s) 10, 16, 273, 389, 460, 795, 982
HspAI GCGC 1 cut(s) 39
KasI GGCGCC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 3 cut(s) 334, 432, 954
LmnI GCTCC 3 cut(s) 146, 394, 589
Lsp1109I GCAGC 6 cut(s) 106, 277, 619, 625, 628, 799
LweI GCATC 2 cut(s) 232, 754
MaeI CTAG 1 cut(s) 129
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 4 cut(s) 136, 272, 580, 794
MalI GATC 3 cut(s) 336, 434, 956
MboI GATC 3 cut(s) 334, 432, 954
MboII GAAGA 5 cut(s) 100, 128, 185, 413, 935
MflI RGATCY 2 cut(s) 432, 954
MhlI GDGCHC 2 cut(s) 71, 456
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 199, 265, 514, 555, 721
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 39
MlyI GAGTC 2 cut(s) 311, 833
MmeI TCCRAC 1 cut(s) 618
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 793
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 554
MslI CAYNNNNRTG 1 cut(s) 11
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 4 cut(s) 106, 595, 610, 619
MspI CCGG 1 cut(s) 866
MspR9I CCNGG 3 cut(s) 30, 144, 648
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 3 cut(s) 30, 144, 648
MwoI GCNNNNNNNGC 6 cut(s) 70, 100, 103, 516, 613, 616
NarI GGCGCC 1 cut(s) 39
NdeI CATATG 1 cut(s) 495
NdeII GATC 3 cut(s) 334, 432, 954
NlaIII CATG 7 cut(s) 10, 16, 273, 389, 460, 795, 982
NlaIV GGNNCC 4 cut(s) 40, 591, 624, 642
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 2 cut(s) 272, 794
NsiI ATGCAT 1 cut(s) 793
NspI RCATGY 3 cut(s) 460, 795, 982
NspV TTCGAA 2 cut(s) 479, 544
PaeR7I CTCGAG 1 cut(s) 984
PciI ACATGT 2 cut(s) 456, 978
PcsI WCGNNNNNNNCGW 3 cut(s) 347, 507, 869
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 6 cut(s) 33, 257, 347, 779, 869, 910
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 9 cut(s) 96, 105, 292, 594, 609, 612, 615, 618, 814
PleI GAGTC 2 cut(s) 310, 832
PluTI GGCGCC 1 cut(s) 42
PpsI GAGTC 2 cut(s) 310, 832
PscI ACATGT 2 cut(s) 456, 978
Psp124BI GAGCTC 1 cut(s) 71
Psp6I CCWGG 3 cut(s) 28, 142, 646
PspFI CCCAGC 1 cut(s) 595
PspGI CCWGG 3 cut(s) 28, 142, 646
PspN4I GGNNCC 4 cut(s) 40, 591, 624, 642
PspPI GGNCC 2 cut(s) 622, 640
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 2 cut(s) 343, 619
PsuI RGATCY 2 cut(s) 432, 954
PvuII CAGCTG 1 cut(s) 619
RsaI GTAC 3 cut(s) 27, 485, 652
RsaNI GTAC 3 cut(s) 26, 484, 651
RseI CAYNNNNRTG 1 cut(s) 11
SacI GAGCTC 1 cut(s) 71
SaqAI TTAA 1 cut(s) 554
SatI GCNGC 9 cut(s) 95, 104, 291, 593, 608, 611, 614, 617, 813
Sau3AI GATC 3 cut(s) 334, 432, 954
Sau96I GGNCC 2 cut(s) 622, 640
SchI GAGTC 2 cut(s) 311, 833
ScrFI CCNGG 3 cut(s) 30, 144, 648
SduI GDGCHC 2 cut(s) 71, 456
SfaNI GCATC 2 cut(s) 232, 754
SfcI CTRYAG 1 cut(s) 654
SfoI GGCGCC 1 cut(s) 40
Sfr274I CTCGAG 1 cut(s) 984
SfuI TTCGAA 2 cut(s) 479, 544
SinI GGWCC 2 cut(s) 622, 640
SlaI CTCGAG 1 cut(s) 984
SmiMI CAYNNNNRTG 1 cut(s) 11
SmlI CTYRAG 1 cut(s) 984
SmoI CTYRAG 1 cut(s) 984
Sse9I AATT 5 cut(s) 199, 265, 514, 555, 721
SsiI CCGC 6 cut(s) 56, 104, 409, 473, 593, 610
SspDI GGCGCC 1 cut(s) 38
SspMI CTAG 1 cut(s) 129
SstI GAGCTC 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 28, 142, 646
StyI CCWWGG 3 cut(s) 128, 222, 744
TaaI ACNGT 4 cut(s) 122, 447, 586, 969
TaiI ACGT 1 cut(s) 580
TaqI TCGA 5 cut(s) 350, 479, 544, 872, 985
TasI AATT 5 cut(s) 199, 265, 514, 555, 721
TauI GCSGC 3 cut(s) 106, 595, 613
TfiI GAWTC 6 cut(s) 33, 257, 347, 779, 869, 910
Tru1I TTAA 1 cut(s) 554
Tru9I TTAA 1 cut(s) 554
TseFI GTSAC 2 cut(s) 272, 794
TseI GCWGC 6 cut(s) 94, 290, 607, 613, 616, 812
Tsp45I GTSAC 2 cut(s) 272, 794
TspGWI ACGGA 2 cut(s) 295, 817
VneI GTGCAC 1 cut(s) 452
VpaK11BI GGWCC 2 cut(s) 622, 640
XapI RAATTY 2 cut(s) 199, 721
XceI RCATGY 3 cut(s) 460, 795, 982
XhoI CTCGAG 1 cut(s) 984
XmaJI CCTAGG 1 cut(s) 128
XspI CTAG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.