MD03G1265800.v1.1

Mitochondrial Rho GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012816
Physical Location & Seq
Reverse (-)
35111068 .. 35116358
5291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1265800.v1.1.491

Sequence Viewer

Length: 1950 bp
ATGTCAGCACTTATGTACACCGGTCAACGCACCGGCGTCCGAGTCGTCGTCGCTGGCGACCGCGGCACCGGCAAGTCGACCTTGATTGCCGCCGTGGCCACCGACGCGTTCGACCCCAACGTCCCTCCCGTGCTTCCCCCAACGCGCCTCGCCGCCGATATGTTTCCAGATTTCGTTCCACTCACCATCATCGACACCTCTTCCAGCTTGGAGAATTGCAGTAAGAGGAACGAAGAACTGAAGCGAGCGGATGTGGTGGTGATAACGTACGCCTGTGATCAGCCGATGACGCTCACTCGTCTCACTACTTACTGGCTGCTCGAGCTTCGCCACTTGGAGGTGAAGGTGCCGGTAATTCTGGTTGGATGCAAGTTAGATTTGCGAAATCCGCAGGAGCAGATGAGCATGGAACAGGTGATGTCACCAATCATGCATCAATTTAGGGAAATTCAGACTTGCATTGAGTGTTCCGCATCCGCTCCAGTTCAGGTTCCCGAAGTGTTTTATTATGCTTCGAAGGCAGTCCTTCATCCAACAGCTCCGTTGTTTGATCAAGAAACGCAAGGTCTGCAACCCCGATGTATTAGAGCTTTGAAAAGGATTTTTACTCTTTGTGATCGTGACATGGACGGTGCCCTTAATGATGAGGAGCTGAATGATTTCCAGGTTAAATGTTTTAATGCTCCGCTACAGCCTGAGGAAATTGTGCGAGTTAAAAGGGTTGTACATGAGAGGAAACCAGAAGGTGTCAATGATATTGGTCTTACCCCCAAGGGGTTCTATTATCTTCATACTCTTTTCATTGAGAGAGAACGTATTGAGACAACTTGGTCTGTCCTCAGAAAATTTGGATATGATGATGATCTAAAACTCAGAGAAGACATTCTCGTACTTCCATCCAAGCGTTCTCCAGATCAGAGCATGGAGCTGACGAATGAGGCTGTTGAGCACCTGAAGGGGATTTTTCGTATAAATGATCATGATAATGATGGAGCCCTTCAACCACGTGAGCTTGATAACCTGTTTTCTACTGCTCCAGAAAATCCTTGGAGTGAAGCTCCTTATAAGGATGCTGCTGAGAGAACGGCATCTGGGAATTTACCTCTTAATGCATTTTTGTCTGAGTGGGCCCTCATGACACTGTTAAATCCAAAGCAAAGTTTGGCGAATTTGATATATGTTGGATACAATGGCAGTCCTGCTTCAGCTATCCATGTTACTAGAAGAAGATCAGTAGATCGTAAAAGGCAAAAAACGGAAAGAAATGTTTTCAGCTGCTTCGTTTTTGGTCCTAAAAATGCAGGAAAATCCACTCTTTTAAATTCATTCATAGGAAGGCCTTTCCCAAAGAGCGAGACTATACCTACTGGTGAGCGTTATGCAGTGAATGTCGTTAACCAGATTGGGGGGAATAAGACTCTTGTACTGAGAGAGATACCAGAAGACAAAGTGAAAACATATTTGTCCGATAAGACCTTTTTGACAGCCTGTGATGTTGCTGTATTTGTCCATGACAGTTCAAGCGAACATTCATGGAAGAGATCAAGAGAACTCCTTGTGGATGTTGCTAAGCAAGTAGAACAAAGTGGCTATGGAGTACCTTGCCTCCTTATTGCTGCAAAGGATGATCTCGGTGCATATCCAATGGCAGTACGAGATTCACTCGTGATAAGTCACGAATTGGGCATTGGGGCACCTGTTCGTGTGAGCATGAAGTTGAGCACTATGAATGATGTGTTTTATAAAATCGTAAATGCAGCTGAGCACCCTCACTTGAGCATTCCTGAAACTGAGGCTATGAGGAACCGCAAGAAGTATCTCCAACTTGTTAATCGATCTCTTATGTGTGTTTCAGATGTACTGTATCAAATTACAGTTGGGGCGGCTGCTGCTGTTGTCGGACTGGCAGCTTATCGTGTCTATGCAGCAAGGAGAAACGCTTCTGGTTAG

Protein Analysis

650

Amino Acids

72.63

Weight (kDa)

6.9

Isoelectric Point (pI)

50.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 14 - 175 1.1e-13 Ras family
EF_assoc_2 PF08356 229 - 313 3.7e-26 EF hand associated
EF_assoc_1 PF08355 350 - 418 3.6e-22 EF hand associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63150
fragaria_vesca FvH4_2g23630
malus_domestica MD03G1265800.v1.1 MD11G1286300.v1.1
prunus_persica Prupe.8G241500_v2.0.a1 Prupe.8G241500_v2.0.a1
pyrus_communis pycom03g21230 pycom11g25380
rosa_chinensis RchiOBHm_Chr6g0291101
rosa_laevigata RLG00000012144
rosa_multiflora Rmu_sc0004642.1_g000016
rosa_roxburghii Rroxscaffold_7G00176380
rosa_rugosa Rorug06G0217500 Rorug06G0217600
rosa_samantha Rh6AG327800 Rh6CG341500 Rh6DG328700
rosa_wichuraiana Rw6G028510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1065, 1743
AasI GACNNNNNNGTC 2 cut(s) 119, 829
AccB1I GGYRCC 4 cut(s) 65, 346, 632, 1693
AccBSI CCGCTC 2 cut(s) 248, 479
AccI GTMKAC 1 cut(s) 77
AccII CGCG 3 cut(s) 63, 107, 145
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 5 cut(s) 447, 845, 1096, 1168, 1321
AcuI CTGAAG 3 cut(s) 260, 974, 1188
AcvI CACGTG 1 cut(s) 1007
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 8 cut(s) 17, 269, 726, 891, 1425, 1599, 1653, 1860
AfiI CCNNNNNNNGG 3 cut(s) 337, 774, 1405
AflIII ACRYGT 1 cut(s) 105
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 3 cut(s) 595, 1001, 1521
AjnI CCWGG 1 cut(s) 663
AjuI GAANNNNNNNTTGG 2 cut(s) 1671, 1703
Alw21I GWGCWC 3 cut(s) 951, 1724, 1767
Alw26I GTCTC 3 cut(s) 305, 815, 1349
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 3 cut(s) 96, 1128, 1337
ApaI GGGCCC 1 cut(s) 1132
ApeKI GCWGC 9 cut(s) 316, 1073, 1275, 1616, 1757, 1886, 1889, 1907, 1925
ApoI RAATTY 5 cut(s) 447, 845, 1096, 1168, 1321
AsiGI ACCGGT 1 cut(s) 20
Asp700I GAANNNNTTC 3 cut(s) 659, 882, 1939
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 3 cut(s) 1128, 1129, 1289
AsuHPI GGTGA 6 cut(s) 175, 271, 352, 414, 427, 1382
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 1 cut(s) 1289
AxyI CCTNAGG 1 cut(s) 696
BaeGI GKGCMC 3 cut(s) 637, 1132, 1696
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 4 cut(s) 65, 346, 632, 1693
BanII GRGCYC 2 cut(s) 997, 1132
BauI CACGAG 1 cut(s) 1664
BbrPI CACGTG 1 cut(s) 1007
BbsI GAAGAC 2 cut(s) 885, 1449
Bbv12I GWGCWC 3 cut(s) 951, 1724, 1767
BbvI GCAGC 9 cut(s) 303, 1060, 1262, 1603, 1769, 1873, 1876, 1919, 1937
BccI CCATC 3 cut(s) 194, 904, 983
BceAI ACGGC 2 cut(s) 77, 1101
BciT130I CCWGG 1 cut(s) 665
BciVI GTATCC 1 cut(s) 1178
BclI TGATCA 3 cut(s) 277, 550, 976
BcoDI GTCTC 3 cut(s) 305, 815, 1349
BfaI CTAG 1 cut(s) 1221
BfmI CTRYAG 1 cut(s) 689
BfuI GTATCC 1 cut(s) 1178
BglI GCCNNNNNGGC 1 cut(s) 95
BlpI GCTNAGC 2 cut(s) 1569, 1761
Bme1390I CCNGG 1 cut(s) 665
Bme18I GGWCC 1 cut(s) 1289
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 3 cut(s) 1128, 1129, 1289
BmiI GGNNCC 8 cut(s) 67, 348, 492, 634, 994, 1130, 1695, 1805
BmrFI CCNGG 1 cut(s) 665
BmsI GCATC 5 cut(s) 356, 442, 482, 1060, 1097
BpiI GAAGAC 2 cut(s) 885, 1449
BplI GAGNNNNNCTC 4 cut(s) 1042, 1074, 1647, 1679
BpmI CTGGAG 3 cut(s) 465, 894, 1020
Bpu1102I GCTNAGC 2 cut(s) 1569, 1761
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 1795
Bsa29I ATCGAT 1 cut(s) 1834
BsaAI YACGTR 1 cut(s) 1007
BsaBI GATNNNNATC 1 cut(s) 861
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 4 cut(s) 61, 93, 771, 1046
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 2 cut(s) 1590, 1620
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 774, 1405
Bse118I RCCGGY 4 cut(s) 20, 32, 68, 349
Bse1I ACTGG 4 cut(s) 317, 482, 1372, 1908
Bse21I CCTNAGG 1 cut(s) 696
Bse8I GATNNNNATC 1 cut(s) 861
BseBI CCWGG 1 cut(s) 665
BseCI ATCGAT 1 cut(s) 1834
BseDI CCNNGG 4 cut(s) 61, 93, 771, 1046
BseGI GGATG 8 cut(s) 256, 371, 473, 529, 896, 1075, 1567, 1630
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 3 cut(s) 337, 774, 1405
BseMII CTCAG 8 cut(s) 687, 853, 886, 1068, 1113, 1418, 1752, 1782
BseNI ACTGG 4 cut(s) 317, 482, 1372, 1908
BseRI GAGGAG 1 cut(s) 662
BseSI GKGCMC 3 cut(s) 637, 1132, 1696
BseXI GCAGC 9 cut(s) 303, 1060, 1262, 1603, 1769, 1873, 1876, 1919, 1937
Bsh1236I CGCG 3 cut(s) 63, 107, 145
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 3 cut(s) 98, 1130, 1339
BshNI GGYRCC 4 cut(s) 65, 346, 632, 1693
BshTI ACCGGT 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 1834
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 3 cut(s) 951, 1724, 1767
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 4 cut(s) 21, 33, 69, 350
BsiWI CGTACG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 3 cut(s) 337, 774, 1405
BsmAI GTCTC 3 cut(s) 305, 815, 1349
BsmBI CGTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 107
BsmI GAATGC 1 cut(s) 1779
BsnI GGCC 3 cut(s) 98, 1130, 1339
BsoBI CYCGRG 1 cut(s) 320
Bsp119I TTCGAA 1 cut(s) 515
Bsp120I GGGCCC 1 cut(s) 1128
Bsp1286I GDGCHC 7 cut(s) 637, 951, 997, 1132, 1696, 1724, 1767
Bsp1407I TGTACA 2 cut(s) 15, 724
Bsp1720I GCTNAGC 2 cut(s) 1569, 1761
BspANI GGCC 3 cut(s) 98, 1130, 1339
BspCNI CTCAG 8 cut(s) 688, 852, 885, 1069, 1114, 1419, 1753, 1783
BspDI ATCGAT 1 cut(s) 1834
BspFNI CGCG 3 cut(s) 63, 107, 145
BspHI TCATGA 2 cut(s) 979, 1134
BspLI GGNNCC 8 cut(s) 67, 348, 492, 634, 994, 1130, 1695, 1805
BspT104I TTCGAA 1 cut(s) 515
BspT107I GGYRCC 4 cut(s) 65, 346, 632, 1693
BsrBI CCGCTC 2 cut(s) 248, 479
BsrFI RCCGGY 4 cut(s) 20, 32, 68, 349
BsrGI TGTACA 2 cut(s) 15, 724
BsrI ACTGG 4 cut(s) 317, 482, 1372, 1908
BssAI RCCGGY 4 cut(s) 20, 32, 68, 349
BssECI CCNNGG 4 cut(s) 61, 93, 771, 1046
BssNI GRCGYC 1 cut(s) 36
BssSI CACGAG 1 cut(s) 1664
BssT1I CCWWGG 2 cut(s) 771, 1046
Bst2BI CACGAG 1 cut(s) 1664
Bst2UI CCWGG 1 cut(s) 665
Bst4CI ACNGT 5 cut(s) 632, 1143, 1517, 1863, 1876
Bst6I CTCTTC 2 cut(s) 205, 1532
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 568
BstAUI TGTACA 2 cut(s) 15, 724
BstBAI YACGTR 1 cut(s) 1007
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 55, 246
BstDEI CTNAG 9 cut(s) 696, 839, 872, 1077, 1122, 1427, 1569, 1761, 1791
BstDSI CCRYGG 2 cut(s) 61, 93
BstF5I GGATG 8 cut(s) 256, 371, 473, 529, 896, 1075, 1567, 1630
BstFNI CGCG 3 cut(s) 63, 107, 145
BstHHI GCGC 1 cut(s) 147
BstMAI GTCTC 3 cut(s) 305, 815, 1349
BstMCI CGRYCG 1 cut(s) 61
BstNI CCWGG 1 cut(s) 665
BstSCI CCNGG 1 cut(s) 663
BstSFI CTRYAG 1 cut(s) 689
BstSLI GKGCMC 3 cut(s) 637, 1132, 1696
BstUI CGCG 3 cut(s) 63, 107, 145
BstV1I GCAGC 9 cut(s) 303, 1060, 1262, 1603, 1769, 1873, 1876, 1919, 1937
BstV2I GAAGAC 2 cut(s) 885, 1449
Bsu15I ATCGAT 1 cut(s) 1834
Bsu36I CCTNAGG 1 cut(s) 696
BsuI GTATCC 1 cut(s) 1178
BsuRI GGCC 3 cut(s) 98, 1130, 1339
BsuTUI ATCGAT 1 cut(s) 1834
BtgI CCRYGG 2 cut(s) 61, 93
BtsCI GGATG 8 cut(s) 256, 371, 473, 529, 896, 1075, 1567, 1630
BtsI GCAGTG 1 cut(s) 1389
BtsIMutI CAGTG 2 cut(s) 1139, 1389
Cac8I GCNNGC 2 cut(s) 55, 246
CciI TCATGA 2 cut(s) 979, 1134
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 4 cut(s) 20, 32, 68, 349
Cfr13I GGNCC 3 cut(s) 1128, 1129, 1289
Cfr42I CCGCGG 1 cut(s) 64
ClaI ATCGAT 1 cut(s) 1834
CseI GACGC 3 cut(s) 25, 113, 298
Csp6I GTAC 8 cut(s) 16, 268, 725, 890, 1424, 1598, 1652, 1859
CspAI ACCGGT 1 cut(s) 20
CviQI GTAC 8 cut(s) 16, 268, 725, 890, 1424, 1598, 1652, 1859
DdeI CTNAG 9 cut(s) 696, 839, 872, 1077, 1122, 1427, 1569, 1761, 1791
DraI TTTAAA 1 cut(s) 1320
DrdI GACNNNNNNGTC 2 cut(s) 119, 829
DseDI GACNNNNNNGTC 2 cut(s) 119, 829
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 205, 1532
EarI CTCTTC 2 cut(s) 205, 1532
Eco130I CCWWGG 2 cut(s) 771, 1046
Eco147I AGGCCT 1 cut(s) 1339
Eco24I GRGCYC 2 cut(s) 997, 1132
Eco47I GGWCC 1 cut(s) 1289
Eco57I CTGAAG 3 cut(s) 260, 974, 1188
Eco72I CACGTG 1 cut(s) 1007
Eco81I CCTNAGG 1 cut(s) 696
Eco88I CYCGRG 1 cut(s) 320
EcoO109I RGGNCCY 1 cut(s) 1129
EcoRII CCWGG 1 cut(s) 663
EcoT14I CCWWGG 2 cut(s) 771, 1046
EcoT22I ATGCAT 2 cut(s) 435, 1114
EcoT38I GRGCYC 2 cut(s) 997, 1132
ErhI CCWWGG 2 cut(s) 771, 1046
Esp3I CGTCTC 1 cut(s) 305
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FaqI GGGAC 1 cut(s) 107
FbaI TGATCA 3 cut(s) 277, 550, 976
FblI GTMKAC 1 cut(s) 77
FokI GGATG 8 cut(s) 263, 378, 460, 516, 883, 1082, 1574, 1637
FriOI GRGCYC 2 cut(s) 997, 1132
FspBI CTAG 1 cut(s) 1221
GlaI GCGC 1 cut(s) 146
GsuI CTGGAG 3 cut(s) 465, 894, 1020
HaeIII GGCC 3 cut(s) 98, 1130, 1339
HapII CCGG 4 cut(s) 21, 33, 69, 350
HgaI GACGC 3 cut(s) 25, 113, 298
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 36
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 3 cut(s) 26, 78, 1396
HindII GTYRAC 3 cut(s) 26, 78, 1396
HinfI GANTC 3 cut(s) 42, 1417, 1658
HpaI GTTAAC 1 cut(s) 1396
HpaII CCGG 4 cut(s) 21, 33, 69, 350
HphI GGTGA 6 cut(s) 175, 271, 352, 414, 427, 1382
Hpy166II GTNNAC 4 cut(s) 18, 26, 78, 1396
Hpy188I TCNGA 9 cut(s) 41, 453, 842, 875, 918, 1123, 1468, 1855, 1901
Hpy8I GTNNAC 4 cut(s) 18, 26, 78, 1396
Hpy99I CGWCG 3 cut(s) 50, 53, 107
HpyAV CCTTC 7 cut(s) 337, 511, 536, 737, 949, 1007, 1329
HpyCH4III ACNGT 5 cut(s) 632, 1143, 1517, 1863, 1876
HpyCH4IV ACGT 4 cut(s) 120, 266, 814, 1006
HpyF3I CTNAG 9 cut(s) 696, 839, 872, 1077, 1122, 1427, 1569, 1761, 1791
HpySE526I ACGT 4 cut(s) 120, 266, 814, 1006
Hsp92I GRCGYC 1 cut(s) 36
HspAI GCGC 1 cut(s) 145
Ksp22I TGATCA 3 cut(s) 277, 550, 976
KspAI GTTAAC 1 cut(s) 1396
KspI CCGCGG 1 cut(s) 64
LmnI GCTCC 9 cut(s) 394, 484, 544, 649, 688, 925, 992, 1039, 1063
Lsp1109I GCAGC 9 cut(s) 303, 1060, 1262, 1603, 1769, 1873, 1876, 1919, 1937
LweI GCATC 5 cut(s) 356, 442, 482, 1060, 1097
MaeI CTAG 1 cut(s) 1221
MaeII ACGT 4 cut(s) 120, 266, 814, 1006
MaeIII GTNAC 4 cut(s) 420, 620, 1216, 1673
MbiI CCGCTC 2 cut(s) 248, 479
MboII GAAGA 8 cut(s) 192, 245, 779, 890, 1236, 1239, 1454, 1549
MhlI GDGCHC 7 cut(s) 637, 951, 997, 1132, 1696, 1724, 1767
MlsI TGGCCA 1 cut(s) 98
MluI ACGCGT 1 cut(s) 105
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 2 cut(s) 51, 1411
MmeI TCCRAC 5 cut(s) 343, 557, 1162, 1846, 1879
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 2 cut(s) 435, 1114
MroXI GAANNNNTTC 3 cut(s) 659, 882, 1939
MscI TGGCCA 1 cut(s) 98
MseI TTAA 9 cut(s) 639, 669, 678, 714, 1107, 1145, 1319, 1395, 1830
MslI CAYNNNNRTG 1 cut(s) 984
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 3 cut(s) 63, 1275, 1760
MspI CCGG 4 cut(s) 21, 33, 69, 350
MspR9I CCNGG 1 cut(s) 665
Mva1269I GAATGC 1 cut(s) 1779
MvaI CCWGG 1 cut(s) 665
MvnI CGCG 3 cut(s) 63, 107, 145
NlaIV GGNNCC 8 cut(s) 67, 348, 492, 634, 994, 1130, 1695, 1805
NmuCI GTSAC 3 cut(s) 420, 620, 1673
NsiI ATGCAT 2 cut(s) 435, 1114
NspV TTCGAA 1 cut(s) 515
PaeR7I CTCGAG 1 cut(s) 320
PagI TCATGA 2 cut(s) 979, 1134
PceI AGGCCT 1 cut(s) 1339
PcsI WCGNNNNNNNCGW 3 cut(s) 54, 117, 126
PctI GAATGC 1 cut(s) 1779
PdmI GAANNNNTTC 3 cut(s) 659, 882, 1939
PfeI GAWTC 1 cut(s) 1658
Pfl23II CGTACG 1 cut(s) 267
PinAI ACCGGT 1 cut(s) 20
PleI GAGTC 2 cut(s) 50, 1411
PmaCI CACGTG 1 cut(s) 1007
PmlI CACGTG 1 cut(s) 1007
PpsI GAGTC 2 cut(s) 50, 1411
Ppu21I YACGTR 1 cut(s) 1007
PsiI TTATAA 2 cut(s) 1065, 1743
Psp6I CCWGG 1 cut(s) 663
PspCI CACGTG 1 cut(s) 1007
PspGI CCWGG 1 cut(s) 663
PspLI CGTACG 1 cut(s) 267
PspN4I GGNNCC 8 cut(s) 67, 348, 492, 634, 994, 1130, 1695, 1805
PspOMI GGGCCC 1 cut(s) 1128
PspPI GGNCC 3 cut(s) 1128, 1129, 1289
PspXI VCTCGAGB 1 cut(s) 320
PvuII CAGCTG 2 cut(s) 1275, 1760
RsaI GTAC 8 cut(s) 17, 269, 726, 891, 1425, 1599, 1653, 1860
RsaNI GTAC 8 cut(s) 16, 268, 725, 890, 1424, 1598, 1652, 1859
RseI CAYNNNNRTG 1 cut(s) 984
SacII CCGCGG 1 cut(s) 64
SalI GTCGAC 1 cut(s) 76
SaqAI TTAA 9 cut(s) 639, 669, 678, 714, 1107, 1145, 1319, 1395, 1830
Sau96I GGNCC 3 cut(s) 1128, 1129, 1289
SchI GAGTC 2 cut(s) 51, 1411
ScrFI CCNGG 1 cut(s) 665
SduI GDGCHC 7 cut(s) 637, 951, 997, 1132, 1696, 1724, 1767
SfaNI GCATC 5 cut(s) 356, 442, 482, 1060, 1097
SfcI CTRYAG 1 cut(s) 689
Sfr274I CTCGAG 1 cut(s) 320
Sfr303I CCGCGG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 515
SgrAI CRCCGGYG 1 cut(s) 32
SgrBI CCGCGG 1 cut(s) 64
SinI GGWCC 1 cut(s) 1289
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 2 cut(s) 320, 1774
SmoI CTYRAG 2 cut(s) 320, 1774
SseBI AGGCCT 1 cut(s) 1339
SspMI CTAG 1 cut(s) 1221
StuI AGGCCT 1 cut(s) 1339
StyD4I CCNGG 1 cut(s) 663
StyI CCWWGG 2 cut(s) 771, 1046
TaaI ACNGT 5 cut(s) 632, 1143, 1517, 1863, 1876
TaiI ACGT 4 cut(s) 123, 269, 817, 1009
TaqI TCGA 6 cut(s) 77, 111, 192, 321, 515, 1834
TatI WGTACW 4 cut(s) 15, 724, 1423, 1858
TauI GCSGC 4 cut(s) 66, 92, 155, 1886
TfiI GAWTC 1 cut(s) 1658
Tru1I TTAA 9 cut(s) 639, 669, 678, 714, 1107, 1145, 1319, 1395, 1830
Tru9I TTAA 9 cut(s) 639, 669, 678, 714, 1107, 1145, 1319, 1395, 1830
TscAI CASTG 2 cut(s) 1146, 1389
TseFI GTSAC 3 cut(s) 420, 620, 1673
TseI GCWGC 9 cut(s) 316, 1073, 1275, 1616, 1757, 1886, 1889, 1907, 1925
Tsp45I GTSAC 3 cut(s) 420, 620, 1673
TspDTI ATGAA 8 cut(s) 518, 779, 790, 1314, 1318, 1521, 1727, 1742
TspGWI ACGGA 2 cut(s) 531, 1271
TspRI CASTG 2 cut(s) 1146, 1389
VpaK11BI GGWCC 1 cut(s) 1289
XapI RAATTY 5 cut(s) 447, 845, 1096, 1168, 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1044
XhoI CTCGAG 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 77
XmnI GAANNNNTTC 3 cut(s) 659, 882, 1939
XspI CTAG 1 cut(s) 1221
Zsp2I ATGCAT 2 cut(s) 435, 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.