Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0012816

Physical Location & Seq

Genomic Coordinates Reverse (-)

40589614 .. 40595217

Gene Structure 5604 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1286300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1929 bp

ATGTCAGCTCTTATGTACACCGGAAAGCGCACCGGCGTCCGAGTCGTCGTCTCCGGCGAACGCGGCACAGGCAAGTCGACCTTGATTGCCGCCGTGGCCACCGACGCTTTCGACCCCGCCGTCCCTCCCGTGCTTCCCCCAACGCGCCTCGCCGCCGATATGTTTCCAGATCACGTTCCCCTCACCATCATCGACACCTCCTCCAGCTTGGAGAATTGCAGTAAGAGGAACGAAGAATTGAAGCGAGCGGATGTGGTGGTGCTAACGTACGCCTGCGATCAGCCGATGACGCTCACTCGTCTGACTACTTACTGGCTGCTCGAGCTTCGGCACTTGGAGGTGAGGGTGCCGGTAATTCTGGTTGGATGCAAGTTAGATTTGCGAAATCCGCAGGAGCAGATGAGCATGGAGCAGGTGATGGCACCAATCATGCATCGGTTTAGGGAAATTCAGACTTGCATTGAGTGTTCCGCATCCGCTCAAGTTCAGGTTCCCGAAGTGTTTCATTATGCTTCAAAGGCAGTCCTTCATCCAATAACTCCATTGTTTGATCAAGAAACGCAAAGTCTGCAACCCCGATGTATTAGAGCTTTGAAAAGGATTTTTACTCTTTGTGATTGCGACATGGACGGTGCCCTTAATGATGAGGAGCTGAATAAATTCCAGGTTGAGTGTTTTAATGCTCCACTACAGCCTGAGGAAATTGTGGGAGTTAAGAGGGTTGTACATGAGAGGAAACCAGAAGGTGTCAATGATATTGGTCTTACGCCCGAGGGGTTCTATTATCTTCATACTCTTTTCATTGAGAGAGAACGTATTGAGACAACTTGGTCTGTCCTCAGAAAATTTGGATATGATGATGGTCTAAAACTCAGGGAAGACCTTCTCGTACTTCCATCCAAGCGTTTTCCAGATCAGAGCATGGAGCTGACGAGTGAGGCTATTGAGCACCTGAAGGGGATCTTTCGTATAAATGATCGTGATAATGATGGAGCCCTTCAACCACATGAGCTTGATAACCTATTTTCTACGGCTCCACAAAATCCTTGGAGTGAAGCTCCTTACGAGGATGCTGCAGAGACAACGGCATCAGGGAATTTACCTCTTAATGCGTTTTTGTCTGAGTGGGCCCTCATGACACTGATAGATCCGAAGCAAAGTTTGGCGAATTTGATATACGTTGGGTTCAATGGCAGTCCTGCTTCAGCGATCCATGTTACTAGAAGAAGAGCAGTAGATCGTAAAAAGCAAAAAACGGAAAGAAATGTTTTCAATTGCTTCGTTTTTGGTCCTAAAAATGCAGGAAAATCCGCTCTTTTAAATTCATTCATAGGAAGGCCTTTCTCAAAGAGCGAGACTATACCTACTGGTGAACGTTATGCAGTGAATGTCATTAACCAGATTGGGGGGAATAAGACTCTTGTACTGAGAGAGATACCAGAAGATGAAGTGAAAACACATTTGTCTGATAAGACCTTTTTGGCAGCCTGTGATGTTGCTGTCTTTGTACATGCCAGTTCGAGCGAACATTCATGGAAGAGATCAAGAGAACTCCTTGTGGAGGTTGCTAAGCAAGGAGAAGAAAGTGGTTTTGGAGTACCTTGCCTCCTTGTTGCTGCAAAGGATGATCTCGGTGCATATCCAATGGCAGTACGAGATTCAGTCGCGATAAGTCACGAACTGGGCATTGAGGCACCTGTTCGTGTGAGCATGAAGTTGAGCACTATGAATGATGTGTTCTATAAAATTGTAAATGTAGCTGAGCACCCTCACTTGAGCATTCCCGAAACTGAGGCTAGGAGGAATCGCAAGAAGTACCTCCAGGTTGTTAATCAATCTCTTTTGTGCGTTTCAGTTGGGGCGGCTATTGCTGTTGTCGGACTGGCAGCTTATCGTGCCTATGCAGCAAGGAGAAACGCTTCTGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008150 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017111 GO:0033993 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046872 GO:0050896 GO:0097305 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04137 ko04214 map04137 map04214

Pfam Domains

Protein Families

EF_assoc_1 EF_assoc_2 Ras

Protein Analysis

643

Amino Acids

71.52

Weight (kDa)

6.19

Isoelectric Point (pI)

53.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ras	PF00071	14 - 175	8.2e-14	Ras family
EF_assoc_2	PF08356	229 - 313	8e-25	EF hand associated
EF_assoc_1	PF08355	350 - 418	2e-22	EF hand associated

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012816)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G63150
fragaria_vesca	FvH4_2g23630
malus_domestica	MD03G1265800.v1.1 MD11G1286300.v1.1
prunus_persica	Prupe.8G241500_v2.0.a1 Prupe.8G241500_v2.0.a1
pyrus_communis	pycom03g21230 pycom11g25380
rosa_chinensis	RchiOBHm_Chr6g0291101
rosa_laevigata	RLG00000012144
rosa_multiflora	Rmu_sc0004642.1_g000016
rosa_roxburghii	Rroxscaffold_7G00176380
rosa_rugosa	Rorug06G0217500 Rorug06G0217600
rosa_samantha	Rh6AG327800 Rh6CG341500 Rh6DG328700
rosa_wichuraiana	Rw6G028510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	403
AasI	GACNNNNNNGTC	2 cut(s)	119, 829
Acc36I	ACCTGC	1 cut(s)	403
AccB1I	GGYRCC	4 cut(s)	346, 421, 632, 1693
AccBSI	CCGCTC	3 cut(s)	248, 479, 1313
AccI	GTMKAC	1 cut(s)	77
AccII	CGCG	3 cut(s)	63, 145, 1667
AclI	AACGTT	1 cut(s)	1375
AclWI	GGATC	3 cut(s)	968, 1142, 1204
AcoI	YGGCCR	1 cut(s)	96
AcsI	RAATTY	6 cut(s)	447, 659, 845, 1096, 1168, 1321
AcuI	CTGAAG	2 cut(s)	974, 1188
AcyI	GRCGYC	1 cut(s)	36
AfaI	GTAC	9 cut(s)	17, 269, 726, 891, 1425, 1509, 1599, 1653, 1817
AfiI	CCNNNNNNNGG	2 cut(s)	1405, 1561
AgsI	TTSAA	6 cut(s)	241, 516, 595, 1001, 1189, 1273
AjnI	CCWGG	2 cut(s)	663, 1821
AjuI	GAANNNNNNNTTGG	4 cut(s)	1145, 1177, 1575, 1607
Alw21I	GWGCWC	3 cut(s)	951, 1724, 1767
Alw26I	GTCTC	4 cut(s)	55, 815, 1073, 1349
AlwI	GGATC	3 cut(s)	968, 1142, 1204
Ama87I	CYCGRG	2 cut(s)	320, 770
AoxI	GGCC	3 cut(s)	96, 1128, 1337
ApaI	GGGCCC	1 cut(s)	1132
ApeKI	GCWGC	6 cut(s)	316, 1073, 1484, 1616, 1886, 1904
ApoI	RAATTY	6 cut(s)	447, 659, 845, 1096, 1168, 1321
Asp700I	GAANNNNTTC	4 cut(s)	501, 659, 882, 1918
AspLEI	GCGC	2 cut(s)	30, 147
AspS9I	GGNCC	3 cut(s)	1128, 1129, 1289
AsuHPI	GGTGA	4 cut(s)	175, 352, 427, 1382
AvaI	CYCGRG	2 cut(s)	320, 770
AvaII	GGWCC	1 cut(s)	1289
AxyI	CCTNAGG	1 cut(s)	696
BaeGI	GKGCMC	2 cut(s)	637, 1132
BalI	TGGCCA	1 cut(s)	98
BanI	GGYRCC	4 cut(s)	346, 421, 632, 1693
BanII	GRGCYC	2 cut(s)	997, 1132
BbsI	GAAGAC	1 cut(s)	885
Bbv12I	GWGCWC	3 cut(s)	951, 1724, 1767
BbvI	GCAGC	6 cut(s)	303, 1060, 1496, 1603, 1898, 1916
BccI	CCATC	5 cut(s)	194, 412, 854, 904, 983
BceAI	ACGGC	4 cut(s)	77, 104, 1047, 1101
BcgI	CGANNNNNNTGC	2 cut(s)	1055, 1089
BciT130I	CCWGG	2 cut(s)	665, 1823
BclI	TGATCA	1 cut(s)	550
BcoDI	GTCTC	4 cut(s)	55, 815, 1073, 1349
BfaI	CTAG	2 cut(s)	1221, 1797
BfmI	CTRYAG	2 cut(s)	689, 1074
BfuAI	ACCTGC	1 cut(s)	403
BglI	GCCNNNNNGGC	1 cut(s)	95
BlpI	GCTNAGC	2 cut(s)	1569, 1761
Bme1390I	CCNGG	2 cut(s)	665, 1823
Bme18I	GGWCC	1 cut(s)	1289
BmeT110I	CYCGRG	2 cut(s)	320, 770
BmgT120I	GGNCC	3 cut(s)	1128, 1129, 1289
BmiI	GGNNCC	8 cut(s)	348, 423, 492, 634, 994, 1035, 1130, 1695
BmrFI	CCNGG	2 cut(s)	665, 1823
BmrI	ACTGGG	1 cut(s)	1691
BmsI	GCATC	5 cut(s)	356, 442, 482, 1060, 1097
BmuI	ACTGGG	1 cut(s)	1691
BpiI	GAAGAC	1 cut(s)	885
BplI	GAGNNNNNCTC	2 cut(s)	1042, 1074
BpmI	CTGGAG	2 cut(s)	187, 1805
Bpu1102I	GCTNAGC	2 cut(s)	1569, 1761
BpuEI	CTTGAG	2 cut(s)	465, 1795
BsaHI	GRCGYC	1 cut(s)	36
BsaJI	CCNNGG	3 cut(s)	93, 771, 1046
BsaWI	WCCGGW	1 cut(s)	20
BsaXI	ACNNNNNCTCC	4 cut(s)	185, 215, 1590, 1620
Bsc4I	CCNNNNNNNGG	2 cut(s)	1405, 1561
Bse118I	RCCGGY	2 cut(s)	32, 349
Bse1I	ACTGG	5 cut(s)	317, 1372, 1515, 1686, 1887
Bse21I	CCTNAGG	1 cut(s)	696
BseBI	CCWGG	2 cut(s)	665, 1823
BseDI	CCNNGG	3 cut(s)	93, 771, 1046
BseGI	GGATG	7 cut(s)	256, 371, 473, 529, 896, 1075, 1630
BseLI	CCNNNNNNNGG	2 cut(s)	1405, 1561
BseMII	CTCAG	7 cut(s)	687, 853, 886, 1113, 1418, 1752, 1782
BseNI	ACTGG	5 cut(s)	317, 1372, 1515, 1686, 1887
BseRI	GAGGAG	2 cut(s)	190, 662
BseSI	GKGCMC	2 cut(s)	637, 1132
BseXI	GCAGC	6 cut(s)	303, 1060, 1496, 1603, 1898, 1916
Bsh1236I	CGCG	3 cut(s)	63, 145, 1667
BshFI	GGCC	3 cut(s)	98, 1130, 1339
BshNI	GGYRCC	4 cut(s)	346, 421, 632, 1693
BsiHKAI	GWGCWC	3 cut(s)	951, 1724, 1767
BsiHKCI	CYCGRG	2 cut(s)	320, 770
BsiSI	CCGG	4 cut(s)	21, 33, 54, 350
BsiWI	CGTACG	1 cut(s)	267
BslFI	GGGAC	1 cut(s)	107
BslI	CCNNNNNNNGG	2 cut(s)	1405, 1561
BsmAI	GTCTC	4 cut(s)	55, 815, 1073, 1349
BsmBI	CGTCTC	1 cut(s)	55
BsmFI	GGGAC	1 cut(s)	107
BsmI	GAATGC	1 cut(s)	1779
BsnI	GGCC	3 cut(s)	98, 1130, 1339
BsoBI	CYCGRG	2 cut(s)	320, 770
Bsp120I	GGGCCC	1 cut(s)	1128
Bsp1286I	GDGCHC	6 cut(s)	637, 951, 997, 1132, 1724, 1767
Bsp1407I	TGTACA	3 cut(s)	15, 724, 1507
Bsp1720I	GCTNAGC	2 cut(s)	1569, 1761
Bsp68I	TCGCGA	1 cut(s)	1667
BspANI	GGCC	3 cut(s)	98, 1130, 1339
BspCNI	CTCAG	7 cut(s)	688, 852, 885, 1114, 1419, 1753, 1783
BspFNI	CGCG	3 cut(s)	63, 145, 1667
BspHI	TCATGA	1 cut(s)	1134
BspLI	GGNNCC	8 cut(s)	348, 423, 492, 634, 994, 1035, 1130, 1695
BspMAI	CTGCAG	1 cut(s)	1078
BspMI	ACCTGC	1 cut(s)	403
BspPI	GGATC	3 cut(s)	968, 1142, 1204
BspQI	GCTCTTC	1 cut(s)	1222
BspT107I	GGYRCC	4 cut(s)	346, 421, 632, 1693
BsrBI	CCGCTC	3 cut(s)	248, 479, 1313
BsrFI	RCCGGY	2 cut(s)	32, 349
BsrGI	TGTACA	3 cut(s)	15, 724, 1507
BsrI	ACTGG	5 cut(s)	317, 1372, 1515, 1686, 1887
BssAI	RCCGGY	2 cut(s)	32, 349
BssECI	CCNNGG	3 cut(s)	93, 771, 1046
BssNI	GRCGYC	1 cut(s)	36
BssT1I	CCWWGG	1 cut(s)	1046
Bst2UI	CCWGG	2 cut(s)	665, 1823
Bst4CI	ACNGT	1 cut(s)	632
Bst6I	CTCTTC	2 cut(s)	1222, 1532
BstACI	GRCGYC	1 cut(s)	36
BstAPI	GCANNNNNTGC	1 cut(s)	568
BstAUI	TGTACA	3 cut(s)	15, 724, 1507
BstC8I	GCNNGC	2 cut(s)	246, 274
BstDEI	CTNAG	8 cut(s)	696, 839, 872, 1122, 1427, 1569, 1761, 1791
BstDSI	CCRYGG	1 cut(s)	93
BstENI	CCTNNNNNAGG	1 cut(s)	1559
BstF5I	GGATG	7 cut(s)	256, 371, 473, 529, 896, 1075, 1630
BstFNI	CGCG	3 cut(s)	63, 145, 1667
BstHHI	GCGC	2 cut(s)	30, 147
BstMAI	GTCTC	4 cut(s)	55, 815, 1073, 1349
BstNI	CCWGG	2 cut(s)	665, 1823
BstNSI	RCATGY	1 cut(s)	1514
BstSCI	CCNGG	2 cut(s)	663, 1821
BstSFI	CTRYAG	2 cut(s)	689, 1074
BstSLI	GKGCMC	2 cut(s)	637, 1132
BstUI	CGCG	3 cut(s)	63, 145, 1667
BstV1I	GCAGC	6 cut(s)	303, 1060, 1496, 1603, 1898, 1916
BstV2I	GAAGAC	1 cut(s)	885
BstX2I	RGATCY	2 cut(s)	960, 1147
BstYI	RGATCY	2 cut(s)	960, 1147
Bsu36I	CCTNAGG	1 cut(s)	696
BsuRI	GGCC	3 cut(s)	98, 1130, 1339
BtgI	CCRYGG	1 cut(s)	93
BtsCI	GGATG	7 cut(s)	256, 371, 473, 529, 896, 1075, 1630
BtsI	GCAGTG	1 cut(s)	1389
BtsIMutI	CAGTG	2 cut(s)	1139, 1389
BtuMI	TCGCGA	1 cut(s)	1667
BveI	ACCTGC	1 cut(s)	403
Cac8I	GCNNGC	2 cut(s)	246, 274
CciI	TCATGA	1 cut(s)	1134
CfoI	GCGC	2 cut(s)	30, 147
Cfr10I	RCCGGY	2 cut(s)	32, 349
Cfr13I	GGNCC	3 cut(s)	1128, 1129, 1289
CseI	GACGC	3 cut(s)	25, 113, 298
Csp6I	GTAC	9 cut(s)	16, 268, 725, 890, 1424, 1508, 1598, 1652, 1816
CviQI	GTAC	9 cut(s)	16, 268, 725, 890, 1424, 1508, 1598, 1652, 1816
DdeI	CTNAG	8 cut(s)	696, 839, 872, 1122, 1427, 1569, 1761, 1791
DraI	TTTAAA	1 cut(s)	1320
DrdI	GACNNNNNNGTC	2 cut(s)	119, 829
DseDI	GACNNNNNNGTC	2 cut(s)	119, 829
EaeI	YGGCCR	1 cut(s)	96
Eam1104I	CTCTTC	2 cut(s)	1222, 1532
EarI	CTCTTC	2 cut(s)	1222, 1532
Eco130I	CCWWGG	1 cut(s)	1046
Eco147I	AGGCCT	1 cut(s)	1339
Eco24I	GRGCYC	2 cut(s)	997, 1132
Eco47I	GGWCC	1 cut(s)	1289
Eco57I	CTGAAG	2 cut(s)	974, 1188
Eco81I	CCTNAGG	1 cut(s)	696
Eco88I	CYCGRG	2 cut(s)	320, 770
EcoNI	CCTNNNNNAGG	1 cut(s)	1559
EcoO109I	RGGNCCY	1 cut(s)	1129
EcoRII	CCWGG	2 cut(s)	663, 1821
EcoT14I	CCWWGG	1 cut(s)	1046
EcoT22I	ATGCAT	1 cut(s)	435
EcoT38I	GRGCYC	2 cut(s)	997, 1132
ErhI	CCWWGG	1 cut(s)	1046
Esp3I	CGTCTC	1 cut(s)	55
FalI	AAGNNNNNCTT	4 cut(s)	65, 97, 947, 979
FaqI	GGGAC	1 cut(s)	107
FauI	CCCGC	1 cut(s)	124
FbaI	TGATCA	1 cut(s)	550
FblI	GTMKAC	1 cut(s)	77
FokI	GGATG	7 cut(s)	263, 378, 460, 516, 883, 1082, 1637
FriOI	GRGCYC	2 cut(s)	997, 1132
FspBI	CTAG	2 cut(s)	1221, 1797
GlaI	GCGC	2 cut(s)	29, 146
GsuI	CTGGAG	2 cut(s)	187, 1805
HaeIII	GGCC	3 cut(s)	98, 1130, 1339
HapII	CCGG	4 cut(s)	21, 33, 54, 350
HgaI	GACGC	3 cut(s)	25, 113, 298
HhaI	GCGC	2 cut(s)	30, 147
Hin1I	GRCGYC	1 cut(s)	36
Hin6I	GCGC	2 cut(s)	28, 145
HinP1I	GCGC	2 cut(s)	28, 145
HincII	GTYRAC	1 cut(s)	78
HindII	GTYRAC	1 cut(s)	78
HinfI	GANTC	4 cut(s)	42, 1417, 1658, 1804
HpaII	CCGG	4 cut(s)	21, 33, 54, 350
HphI	GGTGA	4 cut(s)	175, 352, 427, 1382
Hpy166II	GTNNAC	3 cut(s)	18, 78, 1373
Hpy188I	TCNGA	9 cut(s)	41, 303, 453, 842, 918, 1123, 1152, 1468, 1880
Hpy8I	GTNNAC	3 cut(s)	18, 78, 1373
Hpy99I	CGWCG	2 cut(s)	50, 107
HpyAV	CCTTC	6 cut(s)	536, 737, 893, 949, 1007, 1329
HpyCH4III	ACNGT	1 cut(s)	632
HpyCH4IV	ACGT	5 cut(s)	174, 266, 814, 1179, 1375
HpyF3I	CTNAG	8 cut(s)	696, 839, 872, 1122, 1427, 1569, 1761, 1791
HpySE526I	ACGT	5 cut(s)	174, 266, 814, 1179, 1375
Hsp92I	GRCGYC	1 cut(s)	36
HspAI	GCGC	2 cut(s)	28, 145
Ksp22I	TGATCA	1 cut(s)	550
LguI	GCTCTTC	1 cut(s)	1222
LmnI	GCTCC	8 cut(s)	394, 409, 649, 688, 925, 992, 1039, 1063
Lsp1109I	GCAGC	6 cut(s)	303, 1060, 1496, 1603, 1898, 1916
LweI	GCATC	5 cut(s)	356, 442, 482, 1060, 1097
MaeI	CTAG	2 cut(s)	1221, 1797
MaeII	ACGT	5 cut(s)	174, 266, 814, 1179, 1375
MaeIII	GTNAC	2 cut(s)	1216, 1673
MbiI	CCGCTC	3 cut(s)	248, 479, 1313
MboII	GAAGA	8 cut(s)	245, 779, 890, 1236, 1239, 1454, 1549, 1592
MfeI	CAATTG	1 cut(s)	1273
MflI	RGATCY	2 cut(s)	960, 1147
MhlI	GDGCHC	6 cut(s)	637, 951, 997, 1132, 1724, 1767
MlsI	TGGCCA	1 cut(s)	98
MluNI	TGGCCA	1 cut(s)	98
MlyI	GAGTC	2 cut(s)	51, 1411
MmeI	TCCRAC	2 cut(s)	343, 1858
Mox20I	TGGCCA	1 cut(s)	98
Mph1103I	ATGCAT	1 cut(s)	435
MroXI	GAANNNNTTC	4 cut(s)	501, 659, 882, 1918
MscI	TGGCCA	1 cut(s)	98
MseI	TTAA	7 cut(s)	639, 678, 714, 1107, 1319, 1395, 1830
Msp20I	TGGCCA	1 cut(s)	98
MspI	CCGG	4 cut(s)	21, 33, 54, 350
MspR9I	CCNGG	2 cut(s)	665, 1823
MunI	CAATTG	1 cut(s)	1273
Mva1269I	GAATGC	1 cut(s)	1779
MvaI	CCWGG	2 cut(s)	665, 1823
MvnI	CGCG	3 cut(s)	63, 145, 1667
NlaIV	GGNNCC	8 cut(s)	348, 423, 492, 634, 994, 1035, 1130, 1695
NmuCI	GTSAC	1 cut(s)	1673
NruI	TCGCGA	1 cut(s)	1667
NsiI	ATGCAT	1 cut(s)	435
NspI	RCATGY	1 cut(s)	1514
PaeR7I	CTCGAG	1 cut(s)	320
PagI	TCATGA	1 cut(s)	1134
PaqCI	CACCTGC	1 cut(s)	403
PceI	AGGCCT	1 cut(s)	1339
PciSI	GCTCTTC	1 cut(s)	1222
PcsI	WCGNNNNNNNCGW	2 cut(s)	54, 117
PctI	GAATGC	1 cut(s)	1779
PdmI	GAANNNNTTC	4 cut(s)	501, 659, 882, 1918
PfeI	GAWTC	2 cut(s)	1658, 1804
Pfl23II	CGTACG	1 cut(s)	267
PleI	GAGTC	2 cut(s)	50, 1411
PpsI	GAGTC	2 cut(s)	50, 1411
Psp1406I	AACGTT	1 cut(s)	1375
Psp6I	CCWGG	2 cut(s)	663, 1821
PspGI	CCWGG	2 cut(s)	663, 1821
PspLI	CGTACG	1 cut(s)	267
PspN4I	GGNNCC	8 cut(s)	348, 423, 492, 634, 994, 1035, 1130, 1695
PspOMI	GGGCCC	1 cut(s)	1128
PspPI	GGNCC	3 cut(s)	1128, 1129, 1289
PspXI	VCTCGAGB	1 cut(s)	320
PstI	CTGCAG	1 cut(s)	1078
PsuI	RGATCY	2 cut(s)	960, 1147
RruI	TCGCGA	1 cut(s)	1667
RsaI	GTAC	9 cut(s)	17, 269, 726, 891, 1425, 1509, 1599, 1653, 1817
RsaNI	GTAC	9 cut(s)	16, 268, 725, 890, 1424, 1508, 1598, 1652, 1816
SalI	GTCGAC	1 cut(s)	76
SapI	GCTCTTC	1 cut(s)	1222
SaqAI	TTAA	7 cut(s)	639, 678, 714, 1107, 1319, 1395, 1830
Sau96I	GGNCC	3 cut(s)	1128, 1129, 1289
SchI	GAGTC	2 cut(s)	51, 1411
ScrFI	CCNGG	2 cut(s)	665, 1823
SduI	GDGCHC	6 cut(s)	637, 951, 997, 1132, 1724, 1767
SfaNI	GCATC	5 cut(s)	356, 442, 482, 1060, 1097
SfcI	CTRYAG	2 cut(s)	689, 1074
Sfr274I	CTCGAG	1 cut(s)	320
SgrAI	CRCCGGYG	1 cut(s)	32
SinI	GGWCC	1 cut(s)	1289
SlaI	CTCGAG	1 cut(s)	320
SmlI	CTYRAG	3 cut(s)	320, 480, 1774
SmoI	CTYRAG	3 cut(s)	320, 480, 1774
SseBI	AGGCCT	1 cut(s)	1339
SspMI	CTAG	2 cut(s)	1221, 1797
StuI	AGGCCT	1 cut(s)	1339
StyD4I	CCNGG	2 cut(s)	663, 1821
StyI	CCWWGG	1 cut(s)	1046
TaaI	ACNGT	1 cut(s)	632
TaiI	ACGT	5 cut(s)	177, 269, 817, 1182, 1378
TaqI	TCGA	5 cut(s)	77, 111, 192, 321, 1520
TatI	WGTACW	4 cut(s)	15, 724, 1423, 1507
TauI	GCSGC	4 cut(s)	66, 92, 155, 1865
TfiI	GAWTC	2 cut(s)	1658, 1804
Tru1I	TTAA	7 cut(s)	639, 678, 714, 1107, 1319, 1395, 1830
Tru9I	TTAA	7 cut(s)	639, 678, 714, 1107, 1319, 1395, 1830
TscAI	CASTG	2 cut(s)	1146, 1389
TseFI	GTSAC	1 cut(s)	1673
TseI	GCWGC	6 cut(s)	316, 1073, 1484, 1616, 1886, 1904
Tsp45I	GTSAC	1 cut(s)	1673
TspGWI	ACGGA	1 cut(s)	1271
TspRI	CASTG	2 cut(s)	1146, 1389
VpaK11BI	GGWCC	1 cut(s)	1289
XagI	CCTNNNNNAGG	1 cut(s)	1559
XapI	RAATTY	6 cut(s)	447, 659, 845, 1096, 1168, 1321
XceI	RCATGY	1 cut(s)	1514
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1044
XhoI	CTCGAG	1 cut(s)	320
XmiI	GTMKAC	1 cut(s)	77
XmnI	GAANNNNTTC	4 cut(s)	501, 659, 882, 1918
XspI	CTAG	2 cut(s)	1221, 1797
Zsp2I	ATGCAT	1 cut(s)	435

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.