pycom03g21230

Mitochondrial Rho GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012816
Physical Location & Seq
Reverse (-)
21557287 .. 21561946
4660 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21230.8

Sequence Viewer

Length: 1734 bp
ATGTCAGCACTTATGTACACCGGTCAACGCACCGGCGTCCGAGTCGTCGTCGCTGGCGACCGCGGCACCGGCAAGTCGACCTTGATTACCGCCGTGGCCACCGACGCGTTCGACCCCAACGTCCCTCCCGTGCTTCCCCCAACGCGCCTCGCCGCCGATATGTTTCCAGATTTCGTTCCACTCACCATCATCGACACCTCTTCCAGCTTGGAGAATTGCAGTAAGAGGAACGAAGAACTGAAGCGAGCGGATGTGGTGGTGCTAACGTACGCCTGCGATCAGCCGATGACGCTCACTCGTCTCACTACTTACTGGCTGCTCGAGCTTCGCCACTTGGAGGTGAAGGTGCCAGTAATTCTGGTTGGATGCAAGTTAGATTTGCGAAATCCGCAGGAGCAGATGAGCATGGAGCAGGTGATGTCACCAATCATGCATCAATTTAGAGAAATTCAGACTTGCATTGAGTGTTCCGCATCCGCTCAAGTTCAGGTTCCCGAAGTGTTTTATTATGCTTCAAAGGCAGTCCTTCATCCAACAACTCCGTTGTTTGATCGAGAAACGCAAGGTCTGCAACCCCGATGTATTAGAGCTTTGAAACGGATTTTTACTCTTTGTGATCGTGACATGGACGGTGCCCTTAATGATGAGGAGCTGAATGATTTCCAGGTTAAGTGTTTTAATGCTCCACTACAGCCCGAGGAAATTGTGCGAGTTAAAAGGGTTGTACATGAGAGGAAACCAGAAGGTGTCAATGATATTGGTCTTACCCCCAAGGGGTTCTATTATCTTCATACTCTTTTCATTGAGAGAGAACATATTGAAACAACTTGGTCTGTCCTCAGAAAATTTGGATATGATGATGATCTAAAACTCAGGGAAGACATTCTCGTACTTCCATCCAAGCGTTCTCCAGATCAGAGCATGGAGCTGACGAATGAGGCTGTTGAGCACCTGAAGGGGATCTTTCGTATAAATGATCATGATAATGATGGAGCCCTTCAACCACGTGAGCTTGATAACCTGTTTTCTACTGCTCCAGAATATCCTTGGAGTGAAGCTCCTTATGAGGATGCTGCAGAGAGAACGGCATCAGGGAATTTACCTCTTAATGCATTTTTGTCTGAGTGGGCCCTCATGACACTGTTAAATCCAAAGCAAAGTTTGGCGAATTTGATATATGTTGGATACAATGGCAGTCCTGCTTCAGCGATCGATGTTACTAGGAGAAGATCAGTAGATCGTAAAAAGCAAAAAACGGAAAGAAATGTTTTCAACTGCTTCGTTTTTGGTCCTAAAAATGCAGGAAAATCCGCTCTTTTAAATTCATTCATGGGAAGGCCTTTCTCAAAGAGTGAGACTATACCTACTGGTGAGCGTTATGCAGTGAATGTCGTTAACCAGATTGGGGGGTGTAAGACTCTTGTACTGAGAGAGATACCAGAAGACAAAGTGAAAACATATTTGTCTGATAAGACCTTTTTGACAGCCTGTGATGTTGCTGTATTTGTCCATGACAGTTCGAGCGAACATTCATGGAAGAGAACAAGAGAACTCCTTGTGGATGTTGCTAAGCAAGGAGAAGAAAGTGGCTATGGAGTACCCTGCCTCCTTATTGCTGCAAAGGATCGATCTCGGTGCATATCCAATGGCAGTGCGAGATTCACTCGTGATAAGTCACGAATTGGGCATTGGGGCACCTGTTCGTGTGAGCATGAAGTTGAGCACTATGAATGA

Protein Analysis

578

Amino Acids

65.13

Weight (kDa)

5.99

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012816)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63150
fragaria_vesca FvH4_2g23630
malus_domestica MD03G1265800.v1.1 MD11G1286300.v1.1
prunus_persica Prupe.8G241500_v2.0.a1 Prupe.8G241500_v2.0.a1
pyrus_communis pycom03g21230 pycom11g25380
rosa_chinensis RchiOBHm_Chr6g0291101
rosa_laevigata RLG00000012144
rosa_multiflora Rmu_sc0004642.1_g000016
rosa_roxburghii Rroxscaffold_7G00176380
rosa_rugosa Rorug06G0217500 Rorug06G0217600
rosa_samantha Rh6AG327800 Rh6CG341500 Rh6DG328700
rosa_wichuraiana Rw6G028510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 403
AasI GACNNNNNNGTC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 403
AccB1I GGYRCC 4 cut(s) 65, 346, 632, 1694
AccBSI CCGCTC 3 cut(s) 248, 479, 1313
AccI GTMKAC 1 cut(s) 77
AccII CGCG 3 cut(s) 63, 107, 145
AciI CCGC 9 cut(s) 61, 63, 90, 153, 248, 389, 471, 477, 1311
AclWI GGATC 2 cut(s) 968, 1632
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 5 cut(s) 447, 845, 1096, 1168, 1321
AcuI CTGAAG 3 cut(s) 260, 974, 1188
AcvI CACGTG 1 cut(s) 1007
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 6 cut(s) 17, 269, 726, 891, 1425, 1599
AfiI CCNNNNNNNGG 3 cut(s) 337, 774, 1405
AflIII ACRYGT 1 cut(s) 105
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 5 cut(s) 516, 595, 821, 1001, 1273
AjnI CCWGG 1 cut(s) 663
AjuI GAANNNNNNNTTGG 2 cut(s) 1672, 1704
AluBI AGCT 7 cut(s) 207, 325, 590, 652, 928, 1012, 1058
AluI AGCT 7 cut(s) 207, 325, 590, 652, 928, 1012, 1058
Alw21I GWGCWC 2 cut(s) 951, 1725
Alw26I GTCTC 2 cut(s) 305, 1349
AlwI GGATC 2 cut(s) 968, 1632
Ama87I CYCGRG 2 cut(s) 320, 695
AoxI GGCC 3 cut(s) 96, 1128, 1337
ApaI GGGCCC 1 cut(s) 1132
ApeKI GCWGC 3 cut(s) 316, 1073, 1616
ApoI RAATTY 5 cut(s) 447, 845, 1096, 1168, 1321
AsiGI ACCGGT 1 cut(s) 20
Asp700I GAANNNNTTC 2 cut(s) 659, 882
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 3 cut(s) 1128, 1129, 1289
AsuHPI GGTGA 5 cut(s) 175, 352, 414, 427, 1382
AvaI CYCGRG 2 cut(s) 320, 695
AvaII GGWCC 1 cut(s) 1289
BaeGI GKGCMC 3 cut(s) 637, 1132, 1697
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 4 cut(s) 65, 346, 632, 1694
BanII GRGCYC 2 cut(s) 997, 1132
BauI CACGAG 1 cut(s) 1665
BbrPI CACGTG 1 cut(s) 1007
BbsI GAAGAC 2 cut(s) 885, 1449
Bbv12I GWGCWC 2 cut(s) 951, 1725
BbvI GCAGC 3 cut(s) 303, 1060, 1603
BccI CCATC 3 cut(s) 194, 904, 983
BceAI ACGGC 2 cut(s) 77, 1101
BcgI CGANNNNNNTGC 2 cut(s) 1617, 1651
BciT130I CCWGG 1 cut(s) 665
BciVI GTATCC 1 cut(s) 1178
BclI TGATCA 1 cut(s) 976
BcoDI GTCTC 2 cut(s) 305, 1349
BfaI CTAG 1 cut(s) 1221
BfmI CTRYAG 2 cut(s) 689, 1074
BfuAI ACCTGC 1 cut(s) 403
BfuI GTATCC 1 cut(s) 1178
BisI GCNGC 5 cut(s) 64, 153, 317, 1074, 1617
BlpI GCTNAGC 1 cut(s) 1569
BlsI GCNGC 5 cut(s) 65, 154, 318, 1075, 1618
Bme1390I CCNGG 1 cut(s) 665
Bme18I GGWCC 1 cut(s) 1289
BmeT110I CYCGRG 2 cut(s) 320, 695
BmgT120I GGNCC 3 cut(s) 1128, 1129, 1289
BmiI GGNNCC 7 cut(s) 67, 348, 492, 634, 994, 1130, 1696
BmrFI CCNGG 1 cut(s) 665
BmsI GCATC 5 cut(s) 356, 442, 482, 1060, 1097
BpiI GAAGAC 2 cut(s) 885, 1449
BplI GAGNNNNNCTC 4 cut(s) 1042, 1074, 1648, 1680
BpmI CTGGAG 2 cut(s) 894, 1020
Bpu1102I GCTNAGC 1 cut(s) 1569
BpuEI CTTGAG 1 cut(s) 465
Bsa29I ATCGAT 2 cut(s) 1212, 1627
BsaAI YACGTR 1 cut(s) 1007
BsaBI GATNNNNATC 1 cut(s) 861
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 5 cut(s) 61, 93, 696, 771, 1046
BsaWI WCCGGW 1 cut(s) 20
BsaXI ACNNNNNCTCC 2 cut(s) 1590, 1620
Bsc4I CCNNNNNNNGG 3 cut(s) 337, 774, 1405
Bse118I RCCGGY 3 cut(s) 20, 32, 68
Bse1I ACTGG 3 cut(s) 317, 350, 1372
Bse8I GATNNNNATC 1 cut(s) 861
BseBI CCWGG 1 cut(s) 665
BseCI ATCGAT 2 cut(s) 1212, 1627
BseDI CCNNGG 5 cut(s) 61, 93, 696, 771, 1046
BseGI GGATG 7 cut(s) 256, 371, 473, 529, 896, 1075, 1567
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 3 cut(s) 337, 774, 1405
BseMII CTCAG 4 cut(s) 853, 886, 1113, 1418
BseNI ACTGG 3 cut(s) 317, 350, 1372
BseRI GAGGAG 1 cut(s) 662
BseSI GKGCMC 3 cut(s) 637, 1132, 1697
BseXI GCAGC 3 cut(s) 303, 1060, 1603
Bsh1236I CGCG 3 cut(s) 63, 107, 145
Bsh1285I CGRYCG 2 cut(s) 61, 1212
BshFI GGCC 3 cut(s) 98, 1130, 1339
BshNI GGYRCC 4 cut(s) 65, 346, 632, 1694
BshTI ACCGGT 1 cut(s) 20
BshVI ATCGAT 2 cut(s) 1212, 1627
BsiEI CGRYCG 2 cut(s) 61, 1212
BsiHKAI GWGCWC 2 cut(s) 951, 1725
BsiHKCI CYCGRG 2 cut(s) 320, 695
BsiSI CCGG 3 cut(s) 21, 33, 69
BsiWI CGTACG 1 cut(s) 267
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 3 cut(s) 337, 774, 1405
BsmAI GTCTC 2 cut(s) 305, 1349
BsmBI CGTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 3 cut(s) 98, 1130, 1339
BsoBI CYCGRG 2 cut(s) 320, 695
Bsp120I GGGCCC 1 cut(s) 1128
Bsp1286I GDGCHC 6 cut(s) 637, 951, 997, 1132, 1697, 1725
Bsp1407I TGTACA 2 cut(s) 15, 724
Bsp1720I GCTNAGC 1 cut(s) 1569
BspACI CCGC 9 cut(s) 61, 63, 90, 153, 248, 389, 471, 477, 1311
BspANI GGCC 3 cut(s) 98, 1130, 1339
BspCNI CTCAG 4 cut(s) 852, 885, 1114, 1419
BspDI ATCGAT 2 cut(s) 1212, 1627
BspFNI CGCG 3 cut(s) 63, 107, 145
BspHI TCATGA 2 cut(s) 979, 1134
BspLI GGNNCC 7 cut(s) 67, 348, 492, 634, 994, 1130, 1696
BspMAI CTGCAG 1 cut(s) 1078
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 2 cut(s) 968, 1632
BspT107I GGYRCC 4 cut(s) 65, 346, 632, 1694
BsrBI CCGCTC 3 cut(s) 248, 479, 1313
BsrFI RCCGGY 3 cut(s) 20, 32, 68
BsrGI TGTACA 2 cut(s) 15, 724
BsrI ACTGG 3 cut(s) 317, 350, 1372
BssAI RCCGGY 3 cut(s) 20, 32, 68
BssECI CCNNGG 5 cut(s) 61, 93, 696, 771, 1046
BssNI GRCGYC 1 cut(s) 36
BssSI CACGAG 1 cut(s) 1665
BssT1I CCWWGG 2 cut(s) 771, 1046
Bst2BI CACGAG 1 cut(s) 1665
Bst2UI CCWGG 1 cut(s) 665
Bst4CI ACNGT 3 cut(s) 632, 1143, 1517
Bst6I CTCTTC 2 cut(s) 205, 1532
BstACI GRCGYC 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 568
BstAUI TGTACA 2 cut(s) 15, 724
BstBAI YACGTR 1 cut(s) 1007
BstC8I GCNNGC 3 cut(s) 55, 246, 274
BstDEI CTNAG 5 cut(s) 839, 872, 1122, 1427, 1569
BstDSI CCRYGG 2 cut(s) 61, 93
BstF5I GGATG 7 cut(s) 256, 371, 473, 529, 896, 1075, 1567
BstFNI CGCG 3 cut(s) 63, 107, 145
BstHHI GCGC 1 cut(s) 147
BstMAI GTCTC 2 cut(s) 305, 1349
BstMCI CGRYCG 2 cut(s) 61, 1212
BstMWI GCNNNNNNNGC 8 cut(s) 63, 69, 104, 289, 322, 388, 518, 568
BstNI CCWGG 1 cut(s) 665
BstSCI CCNGG 1 cut(s) 663
BstSFI CTRYAG 2 cut(s) 689, 1074
BstSLI GKGCMC 3 cut(s) 637, 1132, 1697
BstUI CGCG 3 cut(s) 63, 107, 145
BstV1I GCAGC 3 cut(s) 303, 1060, 1603
BstV2I GAAGAC 2 cut(s) 885, 1449
BstX2I RGATCY 1 cut(s) 960
BstYI RGATCY 1 cut(s) 960
Bsu15I ATCGAT 2 cut(s) 1212, 1627
BsuI GTATCC 1 cut(s) 1178
BsuRI GGCC 3 cut(s) 98, 1130, 1339
BsuTUI ATCGAT 2 cut(s) 1212, 1627
BtgI CCRYGG 2 cut(s) 61, 93
BtsCI GGATG 7 cut(s) 256, 371, 473, 529, 896, 1075, 1567
BtsI GCAGTG 2 cut(s) 1389, 1657
BtsIMutI CAGTG 3 cut(s) 1139, 1389, 1657
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 3 cut(s) 55, 246, 274
CciI TCATGA 2 cut(s) 979, 1134
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 3 cut(s) 20, 32, 68
Cfr13I GGNCC 3 cut(s) 1128, 1129, 1289
Cfr42I CCGCGG 1 cut(s) 64
ClaI ATCGAT 2 cut(s) 1212, 1627
CseI GACGC 3 cut(s) 25, 113, 298
Csp6I GTAC 6 cut(s) 16, 268, 725, 890, 1424, 1598
CspAI ACCGGT 1 cut(s) 20
CviQI GTAC 6 cut(s) 16, 268, 725, 890, 1424, 1598
DdeI CTNAG 5 cut(s) 839, 872, 1122, 1427, 1569
DraI TTTAAA 1 cut(s) 1320
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 205, 1532
EarI CTCTTC 2 cut(s) 205, 1532
Eco130I CCWWGG 2 cut(s) 771, 1046
Eco147I AGGCCT 1 cut(s) 1339
Eco24I GRGCYC 2 cut(s) 997, 1132
Eco47I GGWCC 1 cut(s) 1289
Eco57I CTGAAG 3 cut(s) 260, 974, 1188
Eco72I CACGTG 1 cut(s) 1007
Eco88I CYCGRG 2 cut(s) 320, 695
EcoO109I RGGNCCY 1 cut(s) 1129
EcoRII CCWGG 1 cut(s) 663
EcoT14I CCWWGG 2 cut(s) 771, 1046
EcoT22I ATGCAT 2 cut(s) 435, 1114
EcoT38I GRGCYC 2 cut(s) 997, 1132
ErhI CCWWGG 2 cut(s) 771, 1046
Esp3I CGTCTC 1 cut(s) 305
FalI AAGNNNNNCTT 4 cut(s) 65, 97, 947, 979
FaqI GGGAC 1 cut(s) 107
FbaI TGATCA 1 cut(s) 976
FblI GTMKAC 1 cut(s) 77
Fnu4HI GCNGC 5 cut(s) 64, 153, 317, 1074, 1617
FokI GGATG 7 cut(s) 263, 378, 460, 516, 883, 1082, 1574
FriOI GRGCYC 2 cut(s) 997, 1132
Fsp4HI GCNGC 5 cut(s) 64, 153, 317, 1074, 1617
FspBI CTAG 1 cut(s) 1221
GlaI GCGC 1 cut(s) 146
GluI GCNGC 5 cut(s) 64, 153, 317, 1074, 1617
GsuI CTGGAG 2 cut(s) 894, 1020
HaeIII GGCC 3 cut(s) 98, 1130, 1339
HapII CCGG 3 cut(s) 21, 33, 69
HgaI GACGC 3 cut(s) 25, 113, 298
HhaI GCGC 1 cut(s) 147
Hin1I GRCGYC 1 cut(s) 36
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 3 cut(s) 26, 78, 1396
HindII GTYRAC 3 cut(s) 26, 78, 1396
HinfI GANTC 3 cut(s) 42, 1417, 1659
HpaI GTTAAC 1 cut(s) 1396
HpaII CCGG 3 cut(s) 21, 33, 69
HphI GGTGA 5 cut(s) 175, 352, 414, 427, 1382
Hpy166II GTNNAC 4 cut(s) 18, 26, 78, 1396
Hpy188I TCNGA 6 cut(s) 41, 453, 842, 918, 1123, 1468
Hpy8I GTNNAC 4 cut(s) 18, 26, 78, 1396
Hpy99I CGWCG 3 cut(s) 50, 53, 107
HpyAV CCTTC 6 cut(s) 337, 536, 737, 949, 1007, 1329
HpyCH4III ACNGT 3 cut(s) 632, 1143, 1517
HpyCH4IV ACGT 3 cut(s) 120, 266, 1006
HpyF10VI GCNNNNNNNGC 8 cut(s) 63, 69, 104, 289, 322, 388, 518, 568
HpyF3I CTNAG 5 cut(s) 839, 872, 1122, 1427, 1569
HpySE526I ACGT 3 cut(s) 120, 266, 1006
Hsp92I GRCGYC 1 cut(s) 36
HspAI GCGC 1 cut(s) 145
Ksp22I TGATCA 1 cut(s) 976
KspAI GTTAAC 1 cut(s) 1396
KspI CCGCGG 1 cut(s) 64
LmnI GCTCC 8 cut(s) 394, 409, 649, 688, 925, 992, 1039, 1063
Lsp1109I GCAGC 3 cut(s) 303, 1060, 1603
LweI GCATC 5 cut(s) 356, 442, 482, 1060, 1097
MaeI CTAG 1 cut(s) 1221
MaeII ACGT 3 cut(s) 120, 266, 1006
MaeIII GTNAC 4 cut(s) 420, 620, 1216, 1674
MbiI CCGCTC 3 cut(s) 248, 479, 1313
MboII GAAGA 8 cut(s) 192, 245, 779, 890, 1239, 1454, 1549, 1592
MflI RGATCY 1 cut(s) 960
MhlI GDGCHC 6 cut(s) 637, 951, 997, 1132, 1697, 1725
MlsI TGGCCA 1 cut(s) 98
MluI ACGCGT 1 cut(s) 105
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 2 cut(s) 51, 1411
MmeI TCCRAC 3 cut(s) 343, 557, 1162
Mox20I TGGCCA 1 cut(s) 98
Mph1103I ATGCAT 2 cut(s) 435, 1114
MroXI GAANNNNTTC 2 cut(s) 659, 882
MscI TGGCCA 1 cut(s) 98
MseI TTAA 8 cut(s) 639, 669, 678, 714, 1107, 1145, 1319, 1395
MslI CAYNNNNRTG 1 cut(s) 984
Msp20I TGGCCA 1 cut(s) 98
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 3 cut(s) 21, 33, 69
MspR9I CCNGG 1 cut(s) 665
MvaI CCWGG 1 cut(s) 665
MvnI CGCG 3 cut(s) 63, 107, 145
MwoI GCNNNNNNNGC 8 cut(s) 63, 69, 104, 289, 322, 388, 518, 568
NlaIV GGNNCC 7 cut(s) 67, 348, 492, 634, 994, 1130, 1696
NmuCI GTSAC 3 cut(s) 420, 620, 1674
NsiI ATGCAT 2 cut(s) 435, 1114
PaeR7I CTCGAG 1 cut(s) 320
PagI TCATGA 2 cut(s) 979, 1134
PaqCI CACCTGC 1 cut(s) 403
PceI AGGCCT 1 cut(s) 1339
PcsI WCGNNNNNNNCGW 3 cut(s) 54, 117, 126
PdmI GAANNNNTTC 2 cut(s) 659, 882
PfeI GAWTC 1 cut(s) 1659
Pfl23II CGTACG 1 cut(s) 267
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 5 cut(s) 65, 154, 318, 1075, 1618
Ple19I CGATCG 1 cut(s) 1212
PleI GAGTC 2 cut(s) 50, 1411
PmaCI CACGTG 1 cut(s) 1007
PmlI CACGTG 1 cut(s) 1007
PpsI GAGTC 2 cut(s) 50, 1411
Ppu21I YACGTR 1 cut(s) 1007
Psp6I CCWGG 1 cut(s) 663
PspCI CACGTG 1 cut(s) 1007
PspGI CCWGG 1 cut(s) 663
PspLI CGTACG 1 cut(s) 267
PspN4I GGNNCC 7 cut(s) 67, 348, 492, 634, 994, 1130, 1696
PspOMI GGGCCC 1 cut(s) 1128
PspPI GGNCC 3 cut(s) 1128, 1129, 1289
PspXI VCTCGAGB 1 cut(s) 320
PstI CTGCAG 1 cut(s) 1078
PsuI RGATCY 1 cut(s) 960
PvuI CGATCG 1 cut(s) 1212
RsaI GTAC 6 cut(s) 17, 269, 726, 891, 1425, 1599
RsaNI GTAC 6 cut(s) 16, 268, 725, 890, 1424, 1598
RseI CAYNNNNRTG 1 cut(s) 984
SacII CCGCGG 1 cut(s) 64
SalI GTCGAC 1 cut(s) 76
SaqAI TTAA 8 cut(s) 639, 669, 678, 714, 1107, 1145, 1319, 1395
SatI GCNGC 5 cut(s) 64, 153, 317, 1074, 1617
Sau96I GGNCC 3 cut(s) 1128, 1129, 1289
SchI GAGTC 2 cut(s) 51, 1411
ScrFI CCNGG 1 cut(s) 665
SduI GDGCHC 6 cut(s) 637, 951, 997, 1132, 1697, 1725
SfaNI GCATC 5 cut(s) 356, 442, 482, 1060, 1097
SfcI CTRYAG 2 cut(s) 689, 1074
Sfr274I CTCGAG 1 cut(s) 320
Sfr303I CCGCGG 1 cut(s) 64
SgrAI CRCCGGYG 1 cut(s) 32
SgrBI CCGCGG 1 cut(s) 64
SinI GGWCC 1 cut(s) 1289
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 984
SmlI CTYRAG 2 cut(s) 320, 480
SmoI CTYRAG 2 cut(s) 320, 480
SseBI AGGCCT 1 cut(s) 1339
SsiI CCGC 9 cut(s) 61, 63, 90, 153, 248, 389, 471, 477, 1311
SspMI CTAG 1 cut(s) 1221
StuI AGGCCT 1 cut(s) 1339
StyD4I CCNGG 1 cut(s) 663
StyI CCWWGG 2 cut(s) 771, 1046
TaaI ACNGT 3 cut(s) 632, 1143, 1517
TaiI ACGT 3 cut(s) 123, 269, 1009
TaqI TCGA 8 cut(s) 77, 111, 192, 321, 553, 1212, 1520, 1627
TatI WGTACW 3 cut(s) 15, 724, 1423
TauI GCSGC 2 cut(s) 66, 155
TfiI GAWTC 1 cut(s) 1659
Tru1I TTAA 8 cut(s) 639, 669, 678, 714, 1107, 1145, 1319, 1395
Tru9I TTAA 8 cut(s) 639, 669, 678, 714, 1107, 1145, 1319, 1395
TscAI CASTG 3 cut(s) 1146, 1389, 1657
TseFI GTSAC 3 cut(s) 420, 620, 1674
TseI GCWGC 3 cut(s) 316, 1073, 1616
Tsp45I GTSAC 3 cut(s) 420, 620, 1674
TspDTI ATGAA 7 cut(s) 518, 779, 790, 1314, 1318, 1521, 1728
TspGWI ACGGA 3 cut(s) 531, 613, 1271
TspRI CASTG 3 cut(s) 1146, 1389, 1657
VpaK11BI GGWCC 1 cut(s) 1289
XapI RAATTY 5 cut(s) 447, 845, 1096, 1168, 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1044
XhoI CTCGAG 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 77
XmnI GAANNNNTTC 2 cut(s) 659, 882
XspI CTAG 1 cut(s) 1221
Zsp2I ATGCAT 2 cut(s) 435, 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.