MD04G1001400.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0019626
Physical Location & Seq
Reverse (-)
169895 .. 171159
1265 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1001400.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGTACTTCTACACATACATTTATGTGTTGATGTTCAATATCTCAAAGGAGGGGTTCATTGAATTAGTGAGAAATTCGGGCAACTTCAACTATGCTGCCATTGATTTTCAAAAGCGAGTGCTCAAGAGTTCCGGCATAGGCGAAGAGACGTACATGCCCCGAGTGGTATTCTGCCCAGATTACAAGATAAATCTGAAGGACGGAAGAGAAGAGGCAGCTATGGTGATGTTCGGGGCCATAAATGACCTTTTCGGAGCCACCAAAGTCCGCCCAAAGGACATTAGGATCCTCGTGGTGAACTGTGGAGTTCTGAATACGACCCCATCGCTGTCATCCATTGTGATAAACCATTTCAAGCTGAGGCCTAACGTACAGAGCTTCAACCTTGGTGGAATGGGTTGTGCTGCAGGAATCGTAGCCCTTGATCTTGCCAAAGACCTTTTAAATGCTTATCCTGGCTCATATGCACTGGTGGTCAGCACGGAGGCTGTGTGCTATACATGGTACAAAGGCAATGATTTCGATCTGCTCCTTTCTAATTGCTTCCTTCGAATGGGGGCGGCCGCCATGCTGCTCTCTAGCTCCAGTCTCGATCGATGGCATGCCAAATATGAACTCAAACAGCTGGTTCGAACTCACAAAGGCATGGATGAGAGGAGCTTCAACAGCATACATTTGAAGGAAGATGCAGAGGGCACACAAGGAGTGTCGATGAGCAAAGACGTGATTCAGGTTGGCGGCCGCGCACTCAAGGCCAACATCACCACTATCGGCCCCCTCGTTCTCCCCGTCAGCGAACAATTCCATTTCCTCACCAATTTGCTCTTCAGGAAGAAGAAAACTAATTACAAGCCTTACATTCCTGACTACAAGCTTGCCTTCGAGCACGTGTGCATATTGGCAACTAGCGAGAAGGTGCTCAATGAAATACAGAAGAACCTAGAGCTTACGGAGGACTACATGGAGGCATCCAGGAAAACCCTGGAGCGATTTGGAGACACTTTTAGTAGTAGTGTTTGGTACGAACTCGCTTACTTAGAGGCAACTTCAAAGATCAAGAGGGGTGGTCGGATTTGGCAGCTCACCTTCGGCTCAGGATTCAAGTGTAACAGCGTCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

374

Amino Acids

42.04

Weight (kDa)

8.86

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 13 - 278 3.7e-101 FAE1/Type III polyketide synthase-like protein
Chal_sti_synt_N PF00195 115 - 192 5.6e-06 Chalcone and stilbene synthases, N-terminal domain
ACP_syn_III_C PF08541 296 - 373 2.6e-06 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019626)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52160 AT3G52160
malus_domestica MD04G1001400.v1.1 MD13G1285900.v1.1
prunus_persica Prupe.1G001400_v2.0.a1
pyrus_communis pycom04g00200 pycom420g00270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 744
AciI CCGC 5 cut(s) 268, 560, 564, 738, 742
AclWI GGATC 2 cut(s) 280, 293
AcoI YGGCCR 2 cut(s) 561, 739
AcsI RAATTY 1 cut(s) 73
AcuI CTGAAG 2 cut(s) 215, 811
AcvI CACGTG 1 cut(s) 889
AfaI GTAC 5 cut(s) 5, 152, 372, 506, 1022
AfiI CCNNNNNNNGG 2 cut(s) 274, 553
AflIII ACRYGT 1 cut(s) 888
AjiI CACGTC 1 cut(s) 724
AjnI CCWGG 3 cut(s) 454, 971, 981
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AluBI AGCT 9 cut(s) 218, 358, 378, 582, 625, 660, 874, 946, 1081
AluI AGCT 9 cut(s) 218, 358, 378, 582, 625, 660, 874, 946, 1081
Alw21I GWGCWC 3 cut(s) 123, 888, 921
Alw26I GTCTC 3 cut(s) 140, 593, 990
AlwI GGATC 2 cut(s) 280, 293
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 6 cut(s) 234, 362, 561, 739, 753, 772
ApeKI GCWGC 5 cut(s) 95, 215, 404, 571, 1078
ApoI RAATTY 1 cut(s) 73
AspLEI GCGC 1 cut(s) 746
AspS9I GGNCC 2 cut(s) 234, 773
AsuHPI GGTGA 5 cut(s) 235, 307, 754, 805, 1075
AsuII TTCGAA 2 cut(s) 550, 631
AvaI CYCGRG 1 cut(s) 159
BaeGI GKGCMC 1 cut(s) 698
BamHI GGATCC 1 cut(s) 285
BauI CACGAG 1 cut(s) 290
BbrPI CACGTG 1 cut(s) 889
Bbv12I GWGCWC 3 cut(s) 123, 888, 921
BbvCI CCTCAGC 1 cut(s) 359
BbvI GCAGC 5 cut(s) 82, 227, 391, 558, 1090
BccI CCATC 2 cut(s) 331, 591
BcgI CGANNNNNNTGC 2 cut(s) 502, 536
BciT130I CCWGG 3 cut(s) 456, 973, 983
BcoDI GTCTC 3 cut(s) 140, 593, 990
BfaI CTAG 3 cut(s) 579, 906, 941
BfmI CTRYAG 1 cut(s) 405
BisI GCNGC 9 cut(s) 96, 216, 405, 561, 564, 572, 739, 742, 1079
BlsI GCNGC 9 cut(s) 97, 217, 406, 562, 565, 573, 740, 743, 1080
Bme1390I CCNGG 3 cut(s) 456, 973, 983
BmeT110I CYCGRG 1 cut(s) 159
BmgBI CACGTC 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 234, 773
BmiI GGNNCC 4 cut(s) 235, 256, 287, 775
BmrFI CCNGG 3 cut(s) 456, 973, 983
BmsI GCATC 2 cut(s) 676, 977
BpmI CTGGAG 2 cut(s) 568, 1004
Bpu10I CCTNAGC 2 cut(s) 359, 1093
Bpu14I TTCGAA 2 cut(s) 550, 631
BpuEI CTTGAG 2 cut(s) 107, 734
Bsa29I ATCGAT 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 889
BsaBI GATNNNNATC 1 cut(s) 522
BsaJI CCNNGG 2 cut(s) 385, 981
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 2 cut(s) 274, 553
Bse1I ACTGG 2 cut(s) 474, 585
Bse3DI GCAATG 1 cut(s) 520
Bse8I GATNNNNATC 1 cut(s) 522
BseBI CCWGG 3 cut(s) 456, 973, 983
BseCI ATCGAT 1 cut(s) 595
BseDI CCNNGG 2 cut(s) 385, 981
BseGI GGATG 3 cut(s) 332, 655, 968
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 2 cut(s) 274, 553
BseMI GCAATG 1 cut(s) 520
BseMII CTCAG 2 cut(s) 350, 1107
BseNI ACTGG 2 cut(s) 474, 585
BseRI GAGGAG 1 cut(s) 670
BseSI GKGCMC 1 cut(s) 698
BseX3I CGGCCG 2 cut(s) 561, 739
BseXI GCAGC 5 cut(s) 82, 227, 391, 558, 1090
Bsh1236I CGCG 1 cut(s) 744
Bsh1285I CGRYCG 3 cut(s) 564, 595, 742
BshFI GGCC 6 cut(s) 236, 364, 563, 741, 755, 774
BshVI ATCGAT 1 cut(s) 595
BsiEI CGRYCG 3 cut(s) 564, 595, 742
BsiHKAI GWGCWC 3 cut(s) 123, 888, 921
BsiHKCI CYCGRG 1 cut(s) 159
BsiSI CCGG 1 cut(s) 132
BslI CCNNNNNNNGG 2 cut(s) 274, 553
BsmAI GTCTC 3 cut(s) 140, 593, 990
BsmBI CGTCTC 1 cut(s) 140
BsnI GGCC 6 cut(s) 236, 364, 563, 741, 755, 774
BsoBI CYCGRG 1 cut(s) 159
Bsp119I TTCGAA 2 cut(s) 550, 631
Bsp1286I GDGCHC 4 cut(s) 123, 698, 888, 921
Bsp143I GATC 5 cut(s) 285, 424, 523, 592, 1053
BspACI CCGC 5 cut(s) 268, 560, 564, 738, 742
BspANI GGCC 6 cut(s) 236, 364, 563, 741, 755, 774
BspCNI CTCAG 2 cut(s) 351, 1106
BspDI ATCGAT 1 cut(s) 595
BspFNI CGCG 1 cut(s) 744
BspLI GGNNCC 4 cut(s) 235, 256, 287, 775
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 2 cut(s) 280, 293
BspQI GCTCTTC 1 cut(s) 830
BspT104I TTCGAA 2 cut(s) 550, 631
BsrDI GCAATG 1 cut(s) 520
BsrI ACTGG 2 cut(s) 474, 585
BssECI CCNNGG 2 cut(s) 385, 981
BssMI GATC 5 cut(s) 285, 424, 523, 592, 1053
BssSI CACGAG 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 385
Bst2BI CACGAG 1 cut(s) 290
Bst2UI CCWGG 3 cut(s) 456, 973, 983
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 4 cut(s) 138, 199, 204, 830
BstBAI YACGTR 1 cut(s) 889
BstBI TTCGAA 2 cut(s) 550, 631
BstC8I GCNNGC 2 cut(s) 603, 876
BstDEI CTNAG 3 cut(s) 359, 1036, 1093
BstF5I GGATG 3 cut(s) 332, 655, 968
BstFNI CGCG 1 cut(s) 744
BstHHI GCGC 1 cut(s) 746
BstKTI GATC 5 cut(s) 288, 427, 526, 595, 1056
BstMAI GTCTC 3 cut(s) 140, 593, 990
BstMBI GATC 5 cut(s) 285, 424, 523, 592, 1053
BstMCI CGRYCG 3 cut(s) 564, 595, 742
BstMWI GCNNNNNNNGC 2 cut(s) 666, 752
BstNI CCWGG 3 cut(s) 456, 973, 983
BstNSI RCATGY 2 cut(s) 157, 605
BstSCI CCNGG 3 cut(s) 454, 971, 981
BstSFI CTRYAG 1 cut(s) 405
BstSLI GKGCMC 1 cut(s) 698
BstUI CGCG 1 cut(s) 744
BstV1I GCAGC 5 cut(s) 82, 227, 391, 558, 1090
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BstZI CGGCCG 2 cut(s) 561, 739
Bsu15I ATCGAT 1 cut(s) 595
BsuRI GGCC 6 cut(s) 236, 364, 563, 741, 755, 774
BsuTUI ATCGAT 1 cut(s) 595
BtgZI GCGATG 1 cut(s) 309
BtrI CACGTC 1 cut(s) 724
BtsCI GGATG 3 cut(s) 332, 655, 968
BtsIMutI CAGTG 1 cut(s) 467
Cac8I GCNNGC 2 cut(s) 603, 876
CciNI GCGGCCGC 2 cut(s) 561, 739
CfoI GCGC 1 cut(s) 746
Cfr13I GGNCC 2 cut(s) 234, 773
ClaI ATCGAT 1 cut(s) 595
CseI GACGC 1 cut(s) 1102
Csp6I GTAC 5 cut(s) 4, 151, 371, 505, 1021
CspCI CAANNNNNGTGG 4 cut(s) 370, 405, 1045, 1080
CviAII CATG 6 cut(s) 154, 501, 568, 602, 646, 961
CviQI GTAC 5 cut(s) 4, 151, 371, 505, 1021
DdeI CTNAG 3 cut(s) 359, 1036, 1093
DpnI GATC 5 cut(s) 287, 426, 525, 594, 1055
DpnII GATC 5 cut(s) 285, 424, 523, 592, 1053
DraI TTTAAA 1 cut(s) 444
EaeI YGGCCR 2 cut(s) 561, 739
EagI CGGCCG 2 cut(s) 561, 739
Eam1104I CTCTTC 4 cut(s) 138, 199, 204, 830
EarI CTCTTC 4 cut(s) 138, 199, 204, 830
EciI GGCGGA 1 cut(s) 257
EclXI CGGCCG 2 cut(s) 561, 739
Eco130I CCWWGG 1 cut(s) 385
Eco147I AGGCCT 1 cut(s) 364
Eco52I CGGCCG 2 cut(s) 561, 739
Eco57I CTGAAG 2 cut(s) 215, 811
Eco72I CACGTG 1 cut(s) 889
Eco88I CYCGRG 1 cut(s) 159
EcoRII CCWGG 3 cut(s) 454, 971, 981
EcoT14I CCWWGG 1 cut(s) 385
ErhI CCWWGG 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 6 cut(s) 157, 504, 571, 605, 649, 964
FalI AAGNNNNNCTT 2 cut(s) 863, 895
FatI CATG 6 cut(s) 153, 500, 567, 601, 645, 960
FauNDI CATATG 1 cut(s) 463
Fnu4HI GCNGC 9 cut(s) 96, 216, 405, 561, 564, 572, 739, 742, 1079
FokI GGATG 3 cut(s) 319, 662, 955
Fsp4HI GCNGC 9 cut(s) 96, 216, 405, 561, 564, 572, 739, 742, 1079
FspBI CTAG 3 cut(s) 579, 906, 941
GlaI GCGC 1 cut(s) 745
GluI GCNGC 9 cut(s) 96, 216, 405, 561, 564, 572, 739, 742, 1079
GsuI CTGGAG 2 cut(s) 568, 1004
HaeIII GGCC 6 cut(s) 236, 364, 563, 741, 755, 774
HapII CCGG 1 cut(s) 132
HgaI GACGC 1 cut(s) 1102
HhaI GCGC 1 cut(s) 746
Hin1II CATG 6 cut(s) 157, 504, 571, 605, 649, 964
Hin6I GCGC 1 cut(s) 744
HinP1I GCGC 1 cut(s) 744
HindIII AAGCTT 1 cut(s) 872
HinfI GANTC 3 cut(s) 411, 727, 1098
HpaII CCGG 1 cut(s) 132
HphI GGTGA 5 cut(s) 235, 307, 754, 805, 1075
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 4 cut(s) 195, 254, 312, 1071
Hpy188III TCNNGA 6 cut(s) 124, 590, 829, 863, 1057, 1095
Hpy8I GTNNAC 1 cut(s) 298
Hpy99I CGWCG 1 cut(s) 1118
HpyAV CCTTC 6 cut(s) 190, 557, 673, 889, 907, 1096
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 4 cut(s) 149, 369, 723, 888
HpyCH4V TGCA 4 cut(s) 407, 467, 689, 894
HpyF10VI GCNNNNNNNGC 2 cut(s) 666, 752
HpyF3I CTNAG 3 cut(s) 359, 1036, 1093
HpySE526I ACGT 4 cut(s) 149, 369, 723, 888
Hsp92II CATG 6 cut(s) 157, 504, 571, 605, 649, 964
HspAI GCGC 1 cut(s) 744
Kzo9I GATC 5 cut(s) 285, 424, 523, 592, 1053
LguI GCTCTTC 1 cut(s) 830
LmnI GCTCC 5 cut(s) 254, 534, 587, 657, 985
Lsp1109I GCAGC 5 cut(s) 82, 227, 391, 558, 1090
LweI GCATC 2 cut(s) 676, 977
MaeI CTAG 3 cut(s) 579, 906, 941
MaeII ACGT 4 cut(s) 149, 369, 723, 888
MaeIII GTNAC 1 cut(s) 1106
MalI GATC 5 cut(s) 287, 426, 525, 594, 1055
MboI GATC 5 cut(s) 285, 424, 523, 592, 1053
MboII GAAGA 8 cut(s) 155, 216, 221, 695, 817, 844, 847, 946
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 4 cut(s) 123, 698, 888, 921
MluCI AATT 6 cut(s) 62, 73, 538, 800, 817, 844
MmeI TCCRAC 1 cut(s) 1049
MseI TTAA 1 cut(s) 443
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 456, 973, 983
MvaI CCWGG 3 cut(s) 456, 973, 983
MvnI CGCG 1 cut(s) 744
MwoI GCNNNNNNNGC 2 cut(s) 666, 752
NdeI CATATG 1 cut(s) 463
NdeII GATC 5 cut(s) 285, 424, 523, 592, 1053
NlaIII CATG 6 cut(s) 157, 504, 571, 605, 649, 964
NlaIV GGNNCC 4 cut(s) 235, 256, 287, 775
NotI GCGGCCGC 2 cut(s) 561, 739
NspI RCATGY 2 cut(s) 157, 605
NspV TTCGAA 2 cut(s) 550, 631
PaeI GCATGC 1 cut(s) 605
PceI AGGCCT 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 830
PcsI WCGNNNNNNNCGW 2 cut(s) 777, 786
PfeI GAWTC 3 cut(s) 411, 727, 1098
PfoI TCCNGGA 1 cut(s) 971
PkrI GCNGC 9 cut(s) 97, 217, 406, 562, 565, 573, 740, 743, 1080
Ple19I CGATCG 1 cut(s) 595
PmaCI CACGTG 1 cut(s) 889
PmlI CACGTG 1 cut(s) 889
Ppu21I YACGTR 1 cut(s) 889
Psp6I CCWGG 3 cut(s) 454, 971, 981
PspCI CACGTG 1 cut(s) 889
PspGI CCWGG 3 cut(s) 454, 971, 981
PspN4I GGNNCC 4 cut(s) 235, 256, 287, 775
PspPI GGNCC 2 cut(s) 234, 773
PstI CTGCAG 1 cut(s) 409
PsuI RGATCY 1 cut(s) 285
PvuI CGATCG 1 cut(s) 595
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 5 cut(s) 5, 152, 372, 506, 1022
RsaNI GTAC 5 cut(s) 4, 151, 371, 505, 1021
RseI CAYNNNNRTG 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 830
SaqAI TTAA 1 cut(s) 443
SatI GCNGC 9 cut(s) 96, 216, 405, 561, 564, 572, 739, 742, 1079
Sau3AI GATC 5 cut(s) 285, 424, 523, 592, 1053
Sau96I GGNCC 2 cut(s) 234, 773
ScrFI CCNGG 3 cut(s) 456, 973, 983
SduI GDGCHC 4 cut(s) 123, 698, 888, 921
SfaNI GCATC 2 cut(s) 676, 977
SfcI CTRYAG 1 cut(s) 405
SfuI TTCGAA 2 cut(s) 550, 631
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 122, 749
SmoI CTYRAG 2 cut(s) 122, 749
SphI GCATGC 1 cut(s) 605
Sse9I AATT 6 cut(s) 62, 73, 538, 800, 817, 844
SseBI AGGCCT 1 cut(s) 364
SsiI CCGC 5 cut(s) 268, 560, 564, 738, 742
SspMI CTAG 3 cut(s) 579, 906, 941
StuI AGGCCT 1 cut(s) 364
StyD4I CCNGG 3 cut(s) 454, 971, 981
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 4 cut(s) 152, 372, 726, 891
TaqI TCGA 7 cut(s) 522, 550, 591, 595, 631, 710, 882
TasI AATT 6 cut(s) 62, 73, 538, 800, 817, 844
TatI WGTACW 1 cut(s) 3
TauI GCSGC 4 cut(s) 563, 566, 741, 744
TfiI GAWTC 3 cut(s) 411, 727, 1098
Tru1I TTAA 1 cut(s) 443
Tru9I TTAA 1 cut(s) 443
TscAI CASTG 1 cut(s) 474
TseI GCWGC 5 cut(s) 95, 215, 404, 571, 1078
TspDTI ATGAA 3 cut(s) 46, 627, 939
TspGWI ACGGA 3 cut(s) 216, 497, 965
TspRI CASTG 1 cut(s) 474
XapI RAATTY 1 cut(s) 73
XceI RCATGY 2 cut(s) 157, 605
XcmI CCANNNNNNNNNTGG 1 cut(s) 979
XspI CTAG 3 cut(s) 579, 906, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.