MD04G1013600.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0021185
Physical Location & Seq
Forward (+)
1554014 .. 1555185
1172 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1013600.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGGGAAGGCGAAGCAGTTAGCAAAGGTACTAGGATCACTAGAGGGTTTCACAACCAAAGAGAATTGGGACAGGTTCTTCATCGCCATCCAAGACGGCAACTATGCCTTCGAGTGGTACGAGGACTGGCCTGAGCTCCAAGGCCCACTGCTGTCCAATCTCCCAAAGCCCGAGTCCCAAACCCTACCGCTGCAATTACTCGTGCCCGGTTGCGGTACTTCCCACCTGTCTGAGCACCTCTATGATGCCGGATTTAAGGCCATCACCAACATTGACCTATCTGAGGTCGCCATTTCGGACATGCTGCGCCGCAATGCGCGTATACGCCCTGGTATGAAGTGGCAAGTCATGGACGTCACTGCCATGCAGTTCGAGGATGAGAGCTTTGACGTTGTTGTTGATAAAGGCGGCTTAGATGCTTTGATGGACCCAGAGTTTGGCGTGCAGATGGGCAATCAATTTTTACAAGAGGTTAACAGGGTTCTAAAGTTTGGGGGCAAATTTATTTGCTTTACTTGGGCGTGGTCTGATGTTCTAGGTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

182

Amino Acids

20.41

Weight (kDa)

4.67

Isoelectric Point (pI)

30.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 69 - 170 2.3e-15 Methyltransferase domain
Methyltransf_31 PF13847 69 - 169 1.2e-11 Methyltransferase domain
Methyltransf_25 PF13649 70 - 166 3.7e-13 Methyltransferase domain
Methyltransf_12 PF08242 70 - 168 5.4e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021185)

Species Orthologous Gene IDs
malus_domestica MD04G1013600.v1.1
prunus_persica Prupe.1G159800_v2.0.a1
pyrus_communis pycom04g01060
rosa_multiflora Rmu_sc0005933.1_g000001
rosa_wichuraiana Rw0G005030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 437
AccI GTMKAC 1 cut(s) 322
AccII CGCG 1 cut(s) 319
AciI CCGC 4 cut(s) 188, 213, 310, 408
AclWI GGATC 1 cut(s) 43
AcsI RAATTY 1 cut(s) 500
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 3 cut(s) 30, 119, 217
AfiI CCNNNNNNNGG 4 cut(s) 114, 212, 283, 437
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 2 cut(s) 136, 384
AluI AGCT 2 cut(s) 136, 384
Alw21I GWGCWC 2 cut(s) 138, 237
AlwI GGATC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 3 cut(s) 128, 142, 258
ApeKI GCWGC 2 cut(s) 190, 304
ApoI RAATTY 1 cut(s) 500
ArsI GACNNNNNNTTYG 4 cut(s) 158, 190, 419, 451
AspLEI GCGC 2 cut(s) 309, 319
AspS9I GGNCC 2 cut(s) 143, 427
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 427
BaeGI GKGCMC 1 cut(s) 207
BanII GRGCYC 1 cut(s) 138
BauI CACGAG 1 cut(s) 200
Bbv12I GWGCWC 2 cut(s) 138, 237
BbvI GCAGC 2 cut(s) 177, 291
BccI CCATC 4 cut(s) 95, 269, 418, 442
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 330
BcnI CCSGG 1 cut(s) 207
BfaI CTAG 3 cut(s) 32, 41, 536
BisI GCNGC 4 cut(s) 191, 305, 310, 409
BlsI GCNGC 4 cut(s) 192, 306, 311, 410
Bme1390I CCNGG 2 cut(s) 207, 330
Bme18I GGWCC 1 cut(s) 427
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 2 cut(s) 143, 427
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 2 cut(s) 207, 330
BmsI GCATC 2 cut(s) 235, 406
Bpu10I CCTNAGC 1 cut(s) 132
BpuMI CCSGG 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 2 cut(s) 139, 328
Bsc4I CCNNNNNNNGG 4 cut(s) 114, 212, 283, 437
Bse1I ACTGG 1 cut(s) 131
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 2 cut(s) 139, 328
BseGI GGATG 2 cut(s) 87, 382
BseLI CCNNNNNNNGG 4 cut(s) 114, 212, 283, 437
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 3 cut(s) 123, 222, 273
BseNI ACTGG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 2 cut(s) 177, 291
BsgI GTGCAG 1 cut(s) 464
Bsh1236I CGCG 1 cut(s) 319
BshFI GGCC 3 cut(s) 130, 144, 260
BsiHKAI GWGCWC 2 cut(s) 138, 237
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 207, 249
BslFI GGGAC 2 cut(s) 83, 160
BslI CCNNNNNNNGG 4 cut(s) 114, 212, 283, 437
BsmFI GGGAC 2 cut(s) 83, 160
BsnI GGCC 3 cut(s) 130, 144, 260
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 138, 207, 237
Bsp143I GATC 1 cut(s) 35
BspACI CCGC 4 cut(s) 188, 213, 310, 408
BspANI GGCC 3 cut(s) 130, 144, 260
BspCNI CTCAG 3 cut(s) 124, 223, 274
BspFNI CGCG 1 cut(s) 319
BspLI GGNNCC 1 cut(s) 429
BspPI GGATC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 319
BsrI ACTGG 1 cut(s) 131
BssECI CCNNGG 2 cut(s) 139, 328
BssMI GATC 1 cut(s) 35
BssNAI GTATAC 1 cut(s) 323
BssNI GRCGYC 1 cut(s) 354
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 139
Bst1107I GTATAC 1 cut(s) 323
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 354
BstC8I GCNNGC 1 cut(s) 443
BstDEI CTNAG 4 cut(s) 132, 231, 282, 412
BstENI CCTNNNNNAGG 1 cut(s) 281
BstF5I GGATG 2 cut(s) 87, 382
BstFNI CGCG 1 cut(s) 319
BstHHI GCGC 2 cut(s) 309, 319
BstKTI GATC 1 cut(s) 38
BstMBI GATC 1 cut(s) 35
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 2 cut(s) 205, 328
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 1 cut(s) 319
BstV1I GCAGC 2 cut(s) 177, 291
BstZ17I GTATAC 1 cut(s) 323
BsuRI GGCC 3 cut(s) 130, 144, 260
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 2 cut(s) 87, 382
BtsI GCAGTG 2 cut(s) 146, 357
BtsIMutI CAGTG 2 cut(s) 146, 357
Cac8I GCNNGC 1 cut(s) 443
CfoI GCGC 2 cut(s) 309, 319
Cfr13I GGNCC 2 cut(s) 143, 427
Csp6I GTAC 3 cut(s) 29, 118, 216
CviAII CATG 3 cut(s) 301, 349, 364
CviJI RGCY 7 cut(s) 130, 136, 144, 169, 260, 384, 411
CviKI_1 RGCY 7 cut(s) 130, 136, 144, 169, 260, 384, 411
CviQI GTAC 3 cut(s) 29, 118, 216
DdeI CTNAG 4 cut(s) 132, 231, 282, 412
DpnI GATC 1 cut(s) 37
DpnII GATC 1 cut(s) 35
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 136
EcoNI CCTNNNNNAGG 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 3 cut(s) 304, 352, 367
FaiI YATR 7 cut(s) 105, 243, 302, 323, 335, 350, 365
FaqI GGGAC 2 cut(s) 83, 160
FatI CATG 3 cut(s) 300, 348, 363
FblI GTMKAC 1 cut(s) 322
Fnu4HI GCNGC 4 cut(s) 191, 305, 310, 409
FokI GGATG 2 cut(s) 74, 389
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 4 cut(s) 191, 305, 310, 409
FspBI CTAG 3 cut(s) 32, 41, 536
GlaI GCGC 2 cut(s) 308, 318
GluI GCNGC 4 cut(s) 191, 305, 310, 409
HaeIII GGCC 3 cut(s) 130, 144, 260
HapII CCGG 2 cut(s) 207, 249
HhaI GCGC 2 cut(s) 309, 319
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 3 cut(s) 304, 352, 367
Hin6I GCGC 2 cut(s) 307, 317
HinP1I GCGC 2 cut(s) 307, 317
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HinfI GANTC 1 cut(s) 173
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 2 cut(s) 207, 249
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 2 cut(s) 323, 475
Hpy188I TCNGA 4 cut(s) 232, 283, 298, 529
Hpy8I GTNNAC 2 cut(s) 323, 475
HpyAV CCTTC 1 cut(s) 118
HpyCH4IV ACGT 2 cut(s) 354, 390
HpyCH4V TGCA 3 cut(s) 193, 367, 445
HpyF3I CTNAG 4 cut(s) 132, 231, 282, 412
HpySE526I ACGT 2 cut(s) 354, 390
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 3 cut(s) 304, 352, 367
HspAI GCGC 2 cut(s) 307, 317
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 2 cut(s) 177, 291
LweI GCATC 2 cut(s) 235, 406
MaeI CTAG 3 cut(s) 32, 41, 536
MaeII ACGT 2 cut(s) 354, 390
MaeIII GTNAC 1 cut(s) 355
MalI GATC 1 cut(s) 37
MboI GATC 1 cut(s) 35
MboII GAAGA 1 cut(s) 70
MhlI GDGCHC 3 cut(s) 138, 207, 237
MluCI AATT 4 cut(s) 64, 194, 458, 500
MlyI GAGTC 1 cut(s) 182
MnlI CCTC 6 cut(s) 37, 115, 248, 277, 367, 463
MseI TTAA 2 cut(s) 255, 474
MslI CAYNNNNRTG 2 cut(s) 240, 362
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 2 cut(s) 207, 249
MspR9I CCNGG 2 cut(s) 207, 330
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 1 cut(s) 319
NciI CCSGG 1 cut(s) 207
NdeII GATC 1 cut(s) 35
NlaIII CATG 3 cut(s) 304, 352, 367
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 4 cut(s) 192, 306, 311, 410
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 2 cut(s) 143, 427
RsaI GTAC 3 cut(s) 30, 119, 217
RsaNI GTAC 3 cut(s) 29, 118, 216
RseI CAYNNNNRTG 2 cut(s) 240, 362
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 2 cut(s) 255, 474
SatI GCNGC 4 cut(s) 191, 305, 310, 409
Sau3AI GATC 1 cut(s) 35
Sau96I GGNCC 2 cut(s) 143, 427
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 2 cut(s) 207, 330
SduI GDGCHC 3 cut(s) 138, 207, 237
SfaNI GCATC 2 cut(s) 235, 406
SinI GGWCC 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 240, 362
Sse9I AATT 4 cut(s) 64, 194, 458, 500
SsiI CCGC 4 cut(s) 188, 213, 310, 408
SspMI CTAG 3 cut(s) 32, 41, 536
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 2 cut(s) 205, 328
StyI CCWWGG 1 cut(s) 139
TaiI ACGT 2 cut(s) 357, 393
TaqI TCGA 2 cut(s) 111, 372
TasI AATT 4 cut(s) 64, 194, 458, 500
TauI GCSGC 2 cut(s) 312, 411
Tru1I TTAA 2 cut(s) 255, 474
Tru9I TTAA 2 cut(s) 255, 474
TscAI CASTG 2 cut(s) 153, 364
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 2 cut(s) 190, 304
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 2 cut(s) 70, 350
TspRI CASTG 2 cut(s) 153, 364
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 427
XagI CCTNNNNNAGG 1 cut(s) 281
XapI RAATTY 1 cut(s) 500
XceI RCATGY 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 322
XspI CTAG 3 cut(s) 32, 41, 536
ZraI GACGTC 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.