Rw0G005030

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00196
OG0021185
Physical Location & Seq
Reverse (-)
350 .. 1773
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G005030.1

Sequence Viewer

Length: 888 bp
ATGGCCAAGGAGGAGCAGCTAGCGGAGCTTCTGGAAACCCTAGGCGACTTCACAAGCAAAGAGAACTGGGACAAGTTCTTCACAATCCGAGGCACCGAAGACGCCTTCGAGTGGTACGCCGAGTGGTCCGAGCTCCGAGACCCTTTGCTCTCCCACCTCCCGCCAAAACCCCAAATCCTCGTCCCCGGCTGCGGCAGCTCCCGCCTCTCCGAGAACCTCTACGACGCCGGCTTCACCTCCATCACCAACATCGACTTCTCCAAGGTCGCCATTTCCGATTGCCTCCGCCGCAATGTGCGCCTCAGGCCCCTTATGCGGTGGCGCGTCATGGACATGACCGCCATGCAGCTTGAGGATGAGGCATTTGATGCTGTGGTTGACAAAGGTGGATTGGATGCGTTAATGGAGCCGGAGCTTGGCCCTAAGTTAGGGGATCAATATTTATCAGAGGTTAGAAGAGTTTTGAAGTCTGGAGGGAAATTTATTTGCCTTACATTGGCGGAGGCGCATGTTCTGGCTCTGCTTTTCCCGAAGTTCCGGTTTGGGTGGAAAATTAGTGTCCATGCAATACCTCACAAACCGTCTAGTAAGCCAAGTCTTCAAGCGTTTATGGTGGTTGCTGTAAAAGAGATGTCCTCTGAGTTGCAGGAGATCACATCCTCATTCAATAAGTCTTCTCTTGCTTGTAGAGGAAGTCAGGGTCGTGGACTTCTTGAAGCTGTTGAAAATGAGAATCAAATTCGTAGAGAATACTCCTCTGCTTCAGATATACAGCACTCTCTTGAAGACTTAAAACTTGGAGCTAGAGGAGATTTAACTAAGCTTCGCCCGGGTCGTCGTTTTCAGCTTACTTTTGTGGGGGCTCCCACTTCAGTTGCAGAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

295

Amino Acids

33.11

Weight (kDa)

5.85

Isoelectric Point (pI)

47.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_25 PF13649 59 - 159 1.6e-11 Methyltransferase domain
Methyltransf_31 PF13847 60 - 167 5.7e-09 Methyltransferase domain
Methyltransf_11 PF08241 62 - 163 1.4e-13 Methyltransferase domain
Methyltransf_12 PF08242 63 - 161 8e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021185)

Species Orthologous Gene IDs
malus_domestica MD04G1013600.v1.1
prunus_persica Prupe.1G159800_v2.0.a1
pyrus_communis pycom04g01060
rosa_multiflora Rmu_sc0005933.1_g000001
rosa_wichuraiana Rw0G005030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 92
AccII CGCG 1 cut(s) 324
AciI CCGC 9 cut(s) 23, 161, 192, 202, 286, 289, 316, 339, 500
AclWI GGATC 1 cut(s) 441
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 479, 738
AcuI CTGAAG 2 cut(s) 747, 855
AcyI GRCGYC 2 cut(s) 102, 225
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 6 cut(s) 111, 191, 315, 416, 428, 496
AgsI TTSAA 6 cut(s) 466, 602, 667, 716, 725, 785
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 1 cut(s) 441
Ama87I CYCGRG 1 cut(s) 829
AoxI GGCC 3 cut(s) 3, 305, 418
ApeKI GCWGC 4 cut(s) 16, 189, 195, 346
ApoI RAATTY 2 cut(s) 479, 738
AspA2I CCTAGG 1 cut(s) 40
AspLEI GCGC 3 cut(s) 300, 324, 508
AspS9I GGNCC 3 cut(s) 126, 306, 419
AsuC2I CCSGG 3 cut(s) 186, 830, 831
AsuHPI GGTGA 2 cut(s) 226, 235
AsuNHI GCTAGC 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 829
AvaII GGWCC 1 cut(s) 126
AvrII CCTAGG 1 cut(s) 40
AxyI CCTNAGG 1 cut(s) 302
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 2 cut(s) 135, 865
BbsI GAAGAC 4 cut(s) 105, 590, 666, 792
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 4 cut(s) 28, 176, 207, 358
BccI CCATC 1 cut(s) 248
BcnI CCSGG 3 cut(s) 186, 830, 831
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 4 cut(s) 20, 41, 585, 804
BfmI CTRYAG 1 cut(s) 884
BisI GCNGC 6 cut(s) 17, 190, 193, 196, 289, 347
BlnI CCTAGG 1 cut(s) 40
BlsI GCNGC 6 cut(s) 18, 191, 194, 197, 290, 348
Bme1390I CCNGG 3 cut(s) 186, 830, 831
Bme18I GGWCC 1 cut(s) 126
BmeT110I CYCGRG 1 cut(s) 829
BmgT120I GGNCC 3 cut(s) 126, 306, 419
BmiI GGNNCC 4 cut(s) 94, 308, 408, 864
BmrFI CCNGG 3 cut(s) 186, 830, 831
BmrI ACTGGG 1 cut(s) 76
BmsI GCATC 2 cut(s) 358, 385
BmtI GCTAGC 1 cut(s) 23
BmuI ACTGGG 1 cut(s) 76
BpiI GAAGAC 4 cut(s) 105, 590, 666, 792
BplI GAGNNNNNCTC 2 cut(s) 620, 652
BpmI CTGGAG 1 cut(s) 492
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 3 cut(s) 186, 830, 831
BsaHI GRCGYC 2 cut(s) 102, 225
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 6 cut(s) 6, 40, 88, 184, 261, 829
BsaWI WCCGGW 1 cut(s) 537
Bsc4I CCNNNNNNNGG 6 cut(s) 111, 191, 315, 416, 428, 496
Bse118I RCCGGY 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 71
Bse21I CCTNAGG 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 298
BseDI CCNNGG 6 cut(s) 6, 40, 88, 184, 261, 829
BseGI GGATG 3 cut(s) 361, 400, 656
BseLI CCNNNNNNNGG 6 cut(s) 111, 191, 315, 416, 428, 496
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 2 cut(s) 316, 630
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 3 cut(s) 26, 745, 822
BseXI GCAGC 4 cut(s) 28, 176, 207, 358
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 3 cut(s) 5, 307, 420
BshNI GGYRCC 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 135
BsiHKCI CYCGRG 1 cut(s) 829
BsiSI CCGG 5 cut(s) 186, 228, 410, 538, 830
BslFI GGGAC 2 cut(s) 83, 167
BslI CCNNNNNNNGG 6 cut(s) 111, 191, 315, 416, 428, 496
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 2 cut(s) 83, 167
BsnI GGCC 3 cut(s) 5, 307, 420
Bso31I GGTCTC 1 cut(s) 132
BsoBI CYCGRG 1 cut(s) 829
Bsp1286I GDGCHC 2 cut(s) 135, 865
Bsp143I GATC 2 cut(s) 433, 651
BspACI CCGC 9 cut(s) 23, 161, 192, 202, 286, 289, 316, 339, 500
BspANI GGCC 3 cut(s) 5, 307, 420
BspCNI CTCAG 2 cut(s) 315, 631
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 4 cut(s) 94, 308, 408, 864
BspOI GCTAGC 1 cut(s) 23
BspPI GGATC 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 298
BsrFI RCCGGY 1 cut(s) 227
BsrI ACTGG 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 227
BssECI CCNNGG 6 cut(s) 6, 40, 88, 184, 261, 829
BssMI GATC 2 cut(s) 433, 651
BssNI GRCGYC 2 cut(s) 102, 225
BssT1I CCWWGG 3 cut(s) 6, 40, 261
Bst4CI ACNGT 1 cut(s) 582
Bst6I CTCTTC 1 cut(s) 451
BstACI GRCGYC 2 cut(s) 102, 225
BstAPI GCANNNNNTGC 1 cut(s) 368
BstC8I GCNNGC 2 cut(s) 21, 229
BstDEI CTNAG 4 cut(s) 302, 423, 639, 819
BstENI CCTNNNNNAGG 1 cut(s) 426
BstF5I GGATG 3 cut(s) 361, 400, 656
BstFNI CGCG 1 cut(s) 324
BstHHI GCGC 3 cut(s) 300, 324, 508
BstKTI GATC 2 cut(s) 436, 654
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 433, 651
BstMWI GCNNNNNNNGC 8 cut(s) 25, 195, 201, 288, 297, 304, 313, 368
BstNSI RCATGY 1 cut(s) 512
BstSCI CCNGG 3 cut(s) 184, 828, 829
BstSFI CTRYAG 1 cut(s) 884
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 4 cut(s) 28, 176, 207, 358
BstV2I GAAGAC 4 cut(s) 105, 590, 666, 792
Bsu36I CCTNAGG 1 cut(s) 302
BsuRI GGCC 3 cut(s) 5, 307, 420
BtsCI GGATG 3 cut(s) 361, 400, 656
Cac8I GCNNGC 2 cut(s) 21, 229
CfoI GCGC 3 cut(s) 300, 324, 508
Cfr10I RCCGGY 1 cut(s) 227
Cfr13I GGNCC 3 cut(s) 126, 306, 419
Cfr9I CCCGGG 1 cut(s) 829
CseI GACGC 3 cut(s) 110, 233, 313
Csp6I GTAC 1 cut(s) 115
CspCI CAANNNNNGTGG 1 cut(s) 856
CviAII CATG 5 cut(s) 328, 334, 343, 509, 563
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 4 cut(s) 302, 423, 639, 819
DpnI GATC 2 cut(s) 435, 653
DpnII GATC 2 cut(s) 433, 651
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 451
EarI CTCTTC 1 cut(s) 451
EciI GGCGGA 2 cut(s) 275, 515
Ecl136II GAGCTC 1 cut(s) 133
Eco130I CCWWGG 3 cut(s) 6, 40, 261
Eco24I GRGCYC 2 cut(s) 135, 865
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 126
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 2 cut(s) 747, 855
Eco81I CCTNAGG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 829
EcoICRI GAGCTC 1 cut(s) 133
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoO109I RGGNCCY 1 cut(s) 306
EcoT14I CCWWGG 3 cut(s) 6, 40, 261
EcoT38I GRGCYC 2 cut(s) 135, 865
ErhI CCWWGG 3 cut(s) 6, 40, 261
FaeI CATG 5 cut(s) 331, 337, 346, 512, 566
FaiI YATR 8 cut(s) 314, 329, 335, 344, 510, 564, 611, 770
FaqI GGGAC 2 cut(s) 83, 167
FatI CATG 5 cut(s) 327, 333, 342, 508, 562
FauI CCCGC 2 cut(s) 168, 209
Fnu4HI GCNGC 6 cut(s) 17, 190, 193, 196, 289, 347
FokI GGATG 3 cut(s) 368, 407, 643
FriOI GRGCYC 2 cut(s) 135, 865
Fsp4HI GCNGC 6 cut(s) 17, 190, 193, 196, 289, 347
FspBI CTAG 4 cut(s) 20, 41, 585, 804
GlaI GCGC 3 cut(s) 299, 323, 507
GluI GCNGC 6 cut(s) 17, 190, 193, 196, 289, 347
GsuI CTGGAG 1 cut(s) 492
HaeIII GGCC 3 cut(s) 5, 307, 420
HapII CCGG 5 cut(s) 186, 228, 410, 538, 830
HgaI GACGC 3 cut(s) 110, 233, 313
HhaI GCGC 3 cut(s) 300, 324, 508
Hin1I GRCGYC 2 cut(s) 102, 225
Hin1II CATG 5 cut(s) 331, 337, 346, 512, 566
Hin6I GCGC 3 cut(s) 298, 322, 506
HinP1I GCGC 3 cut(s) 298, 322, 506
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 821
HinfI GANTC 1 cut(s) 733
HpaII CCGG 5 cut(s) 186, 228, 410, 538, 830
HphI GGTGA 2 cut(s) 226, 235
Hpy166II GTNNAC 2 cut(s) 379, 707
Hpy188I TCNGA 8 cut(s) 89, 130, 137, 211, 277, 448, 640, 766
Hpy188III TCNNGA 5 cut(s) 32, 471, 529, 713, 782
Hpy8I GTNNAC 2 cut(s) 379, 707
Hpy99I CGWCG 2 cut(s) 227, 840
HpyAV CCTTC 1 cut(s) 115
HpyCH4III ACNGT 1 cut(s) 582
HpyCH4V TGCA 4 cut(s) 346, 566, 646, 878
HpyF10VI GCNNNNNNNGC 8 cut(s) 25, 195, 201, 288, 297, 304, 313, 368
HpyF3I CTNAG 4 cut(s) 302, 423, 639, 819
Hsp92I GRCGYC 2 cut(s) 102, 225
Hsp92II CATG 5 cut(s) 331, 337, 346, 512, 566
HspAI GCGC 3 cut(s) 298, 322, 506
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Kzo9I GATC 2 cut(s) 433, 651
LmnI GCTCC 8 cut(s) 13, 25, 138, 203, 406, 412, 800, 868
Lsp1109I GCAGC 4 cut(s) 28, 176, 207, 358
LweI GCATC 2 cut(s) 358, 385
MaeI CTAG 4 cut(s) 20, 41, 585, 804
MalI GATC 2 cut(s) 435, 653
MboI GATC 2 cut(s) 433, 651
MboII GAAGA 6 cut(s) 70, 110, 468, 590, 666, 797
MhlI GDGCHC 2 cut(s) 135, 865
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 479, 552, 738
MluNI TGGCCA 1 cut(s) 5
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 227
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 401, 791, 815
MslI CAYNNNNRTG 1 cut(s) 332
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 5 cut(s) 186, 228, 410, 538, 830
MspR9I CCNGG 3 cut(s) 186, 830, 831
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 8 cut(s) 25, 195, 201, 288, 297, 304, 313, 368
NaeI GCCGGC 1 cut(s) 229
NciI CCSGG 3 cut(s) 186, 830, 831
NdeII GATC 2 cut(s) 433, 651
NgoMIV GCCGGC 1 cut(s) 227
NheI GCTAGC 1 cut(s) 19
NlaIII CATG 5 cut(s) 331, 337, 346, 512, 566
NlaIV GGNNCC 4 cut(s) 94, 308, 408, 864
NmeAIII GCCGAG 1 cut(s) 145
NspI RCATGY 1 cut(s) 512
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 832
PdiI GCCGGC 1 cut(s) 229
PfeI GAWTC 1 cut(s) 733
PkrI GCNGC 6 cut(s) 18, 191, 194, 197, 290, 348
Psp124BI GAGCTC 1 cut(s) 135
PspN4I GGNNCC 4 cut(s) 94, 308, 408, 864
PspPI GGNCC 3 cut(s) 126, 306, 419
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 332
SacI GAGCTC 1 cut(s) 135
SaqAI TTAA 3 cut(s) 401, 791, 815
SatI GCNGC 6 cut(s) 17, 190, 193, 196, 289, 347
Sau3AI GATC 2 cut(s) 433, 651
Sau96I GGNCC 3 cut(s) 126, 306, 419
ScrFI CCNGG 3 cut(s) 186, 830, 831
SduI GDGCHC 2 cut(s) 135, 865
SfaNI GCATC 2 cut(s) 358, 385
SfcI CTRYAG 1 cut(s) 884
SinI GGWCC 1 cut(s) 126
SmaI CCCGGG 1 cut(s) 831
SmiMI CAYNNNNRTG 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 3 cut(s) 479, 552, 738
SsiI CCGC 9 cut(s) 23, 161, 192, 202, 286, 289, 316, 339, 500
SspI AATATT 1 cut(s) 440
SspMI CTAG 4 cut(s) 20, 41, 585, 804
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 3 cut(s) 184, 828, 829
StyI CCWWGG 3 cut(s) 6, 40, 261
TaaI ACNGT 1 cut(s) 582
TaqI TCGA 2 cut(s) 108, 252
TasI AATT 3 cut(s) 479, 552, 738
TauI GCSGC 2 cut(s) 195, 291
TfiI GAWTC 1 cut(s) 733
Tru1I TTAA 3 cut(s) 401, 791, 815
Tru9I TTAA 3 cut(s) 401, 791, 815
TseI GCWGC 4 cut(s) 16, 189, 195, 346
TspMI CCCGGG 1 cut(s) 829
VpaK11BI GGWCC 1 cut(s) 126
XagI CCTNNNNNAGG 1 cut(s) 426
XapI RAATTY 2 cut(s) 479, 738
XceI RCATGY 1 cut(s) 512
XmaI CCCGGG 1 cut(s) 829
XmaJI CCTAGG 1 cut(s) 40
XspI CTAG 4 cut(s) 20, 41, 585, 804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.