pycom04g01060

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0021185
Physical Location & Seq
Forward (+)
961376 .. 964064
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g01060.1

Sequence Viewer

Length: 828 bp
ATGGGGAAGGCGAAGCAGTTAGCACAGGTACTAGGATCACTAGAGGGTTTCACAACCAAAGAGAATTGGGACAGGTTCATCATCGCCATCCGAGACGGCAACTATGCCTTCGAGTGGTACGAGGACTGGTCCGAGCTCCAAGGCCCACTGCTGTCCAATCTCCCGAAGCCCGAGTCCCAAACCCTACCGCTGCAAGTACTCGTGCCCGGTTGCGGTACTTCCCACCTGTCTGAGCACCTCTATGATGCCGGATTTAAGGCCATCACCAACATTGACTTATCTGAGGTCGCCATTTCCGACATGCTGCACCGCAATGCGCGTATACGCCCTGGTATGAAGTGGCAAGTCATGGACGTCACTGCCATGCAGTTCGAGGATGAGAGCTTTGACGTTGTTGTTGATAAAGGCGGCCTAGATGCTTTGATGGACCCAGAGTTTGGCGTGCAGATGGGCAATCAATATTTACAAGAGGTTAACAGAGTTCTAAAGTTTGGGGGCAAATTTATTTGCTTTACTTGGGCCTGGTCTGATGTTCTAGGTTCGCTTTTCTCCACCTTCCGCTTCGGCTGGAAAATGGGCTTTCATGCCATTCCTCTGAACCTGTCTACCAAGCCTAAGGGCCATAACCTTCAAACCTTTATGATGGTGGCTGAAAAAGAGATGCCTACTGCACTGCATCAAATTGCAGTATCTTCACTCAAAACTGCTTCTCATGAAAATCAGGGCTGTAGCTTCCAGCTTGAAGCTTTTGAAACTGAGAACCGAATTCGTAGAGAATGCTCTACTTCTGGTACTACTGGCGGTTCTGATGCTGCTCTGCGCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11 Methyltransf_25 Spermine_synth

Protein Analysis

276

Amino Acids

30.77

Weight (kDa)

5.41

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 65 - 170 7.4e-12 Methyltransferase domain
Methyltransf_25 PF13649 66 - 166 1.2e-12 Methyltransferase domain
Methyltransf_11 PF08241 69 - 170 2.4e-14 Methyltransferase domain
Methyltransf_12 PF08242 70 - 168 5.5e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021185)

Species Orthologous Gene IDs
malus_domestica MD04G1013600.v1.1
prunus_persica Prupe.1G159800_v2.0.a1
pyrus_communis pycom04g01060
rosa_multiflora Rmu_sc0005933.1_g000001
rosa_wichuraiana Rw0G005030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 357
AccB7I CCANNNNNTGG 1 cut(s) 437
AccI GTMKAC 2 cut(s) 322, 605
AccII CGCG 2 cut(s) 319, 822
AciI CCGC 6 cut(s) 188, 213, 310, 408, 559, 801
AclWI GGATC 1 cut(s) 43
AcsI RAATTY 2 cut(s) 500, 765
AcyI GRCGYC 1 cut(s) 354
AfaI GTAC 5 cut(s) 30, 119, 198, 217, 793
AfiI CCNNNNNNNGG 3 cut(s) 114, 212, 437
AgsI TTSAA 3 cut(s) 632, 743, 752
AjnI CCWGG 2 cut(s) 328, 521
AluBI AGCT 5 cut(s) 136, 384, 732, 739, 746
AluI AGCT 5 cut(s) 136, 384, 732, 739, 746
Alw21I GWGCWC 2 cut(s) 138, 237
Alw26I GTCTC 1 cut(s) 87
AlwI GGATC 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 5 cut(s) 142, 258, 409, 519, 619
ApeKI GCWGC 3 cut(s) 190, 304, 812
ApoI RAATTY 2 cut(s) 500, 765
ArsI GACNNNNNNTTYG 4 cut(s) 158, 190, 419, 451
AspLEI GCGC 3 cut(s) 319, 822, 824
AspS9I GGNCC 5 cut(s) 129, 143, 427, 519, 619
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 129, 427
AxyI CCTNAGG 1 cut(s) 615
BaeGI GKGCMC 1 cut(s) 207
BanII GRGCYC 1 cut(s) 138
BauI CACGAG 1 cut(s) 200
Bbv12I GWGCWC 2 cut(s) 138, 237
BbvI GCAGC 3 cut(s) 177, 291, 799
BccI CCATC 5 cut(s) 95, 269, 418, 442, 637
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 2 cut(s) 330, 523
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 87
BfaI CTAG 4 cut(s) 32, 41, 413, 536
BfmI CTRYAG 1 cut(s) 727
BisI GCNGC 4 cut(s) 191, 305, 409, 813
BlsI GCNGC 4 cut(s) 192, 306, 410, 814
BmcAI AGTACT 1 cut(s) 198
Bme1390I CCNGG 3 cut(s) 207, 330, 523
Bme18I GGWCC 2 cut(s) 129, 427
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 5 cut(s) 129, 143, 427, 519, 619
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 3 cut(s) 207, 330, 523
BmsI GCATC 5 cut(s) 235, 406, 651, 685, 799
BpuMI CCSGG 1 cut(s) 207
BsaHI GRCGYC 1 cut(s) 354
BsaJI CCNNGG 2 cut(s) 139, 328
Bsc4I CCNNNNNNNGG 3 cut(s) 114, 212, 437
Bse1I ACTGG 2 cut(s) 131, 802
Bse21I CCTNAGG 1 cut(s) 615
Bse3DI GCAATG 1 cut(s) 319
BseBI CCWGG 2 cut(s) 330, 523
BseDI CCNNGG 2 cut(s) 139, 328
BseGI GGATG 2 cut(s) 87, 382
BseLI CCNNNNNNNGG 3 cut(s) 114, 212, 437
BseMI GCAATG 1 cut(s) 319
BseMII CTCAG 3 cut(s) 222, 273, 747
BseNI ACTGG 2 cut(s) 131, 802
BsePI GCGCGC 1 cut(s) 820
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 3 cut(s) 177, 291, 799
BsgI GTGCAG 3 cut(s) 290, 464, 654
Bsh1236I CGCG 2 cut(s) 319, 822
BshFI GGCC 5 cut(s) 144, 260, 411, 521, 621
BsiHKAI GWGCWC 2 cut(s) 138, 237
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 207, 249
BslFI GGGAC 2 cut(s) 83, 160
BslI CCNNNNNNNGG 3 cut(s) 114, 212, 437
BsmAI GTCTC 1 cut(s) 87
BsmBI CGTCTC 1 cut(s) 87
BsmFI GGGAC 2 cut(s) 83, 160
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 5 cut(s) 144, 260, 411, 521, 621
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 138, 207, 237
Bsp143I GATC 1 cut(s) 35
BspACI CCGC 6 cut(s) 188, 213, 310, 408, 559, 801
BspANI GGCC 5 cut(s) 144, 260, 411, 521, 621
BspCNI CTCAG 3 cut(s) 223, 274, 748
BspFNI CGCG 2 cut(s) 319, 822
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 1 cut(s) 429
BspPI GGATC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 319
BsrI ACTGG 2 cut(s) 131, 802
BssECI CCNNGG 2 cut(s) 139, 328
BssHII GCGCGC 1 cut(s) 820
BssMI GATC 1 cut(s) 35
BssNAI GTATAC 1 cut(s) 323
BssNI GRCGYC 1 cut(s) 354
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 139
Bst1107I GTATAC 1 cut(s) 323
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 2 cut(s) 330, 523
BstACI GRCGYC 1 cut(s) 354
BstC8I GCNNGC 2 cut(s) 443, 822
BstDEI CTNAG 4 cut(s) 231, 282, 615, 756
BstF5I GGATG 2 cut(s) 87, 382
BstFNI CGCG 2 cut(s) 319, 822
BstHHI GCGC 3 cut(s) 319, 822, 824
BstKTI GATC 1 cut(s) 38
BstMAI GTCTC 1 cut(s) 87
BstMBI GATC 1 cut(s) 35
BstMWI GCNNNNNNNGC 1 cut(s) 821
BstNI CCWGG 2 cut(s) 330, 523
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 3 cut(s) 205, 328, 521
BstSFI CTRYAG 1 cut(s) 727
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 2 cut(s) 319, 822
BstV1I GCAGC 3 cut(s) 177, 291, 799
BstZ17I GTATAC 1 cut(s) 323
Bsu36I CCTNAGG 1 cut(s) 615
BsuRI GGCC 5 cut(s) 144, 260, 411, 521, 621
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 2 cut(s) 87, 382
BtsI GCAGTG 3 cut(s) 146, 357, 671
BtsIMutI CAGTG 3 cut(s) 146, 357, 671
Cac8I GCNNGC 2 cut(s) 443, 822
CciI TCATGA 1 cut(s) 712
CfoI GCGC 3 cut(s) 319, 822, 824
Cfr13I GGNCC 5 cut(s) 129, 143, 427, 519, 619
Csp6I GTAC 5 cut(s) 29, 118, 197, 216, 792
CviAII CATG 5 cut(s) 301, 349, 364, 584, 713
CviQI GTAC 5 cut(s) 29, 118, 197, 216, 792
DdeI CTNAG 4 cut(s) 231, 282, 615, 756
DpnI GATC 1 cut(s) 37
DpnII GATC 1 cut(s) 35
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 129, 427
Eco53kI GAGCTC 1 cut(s) 136
Eco81I CCTNAGG 1 cut(s) 615
Eco88I CYCGRG 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 765
EcoRII CCWGG 2 cut(s) 328, 521
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 139
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 5 cut(s) 304, 352, 367, 587, 716
FaqI GGGAC 2 cut(s) 83, 160
FatI CATG 5 cut(s) 300, 348, 363, 583, 712
FblI GTMKAC 2 cut(s) 322, 605
Fnu4HI GCNGC 4 cut(s) 191, 305, 409, 813
FokI GGATG 2 cut(s) 74, 389
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 4 cut(s) 191, 305, 409, 813
FspBI CTAG 4 cut(s) 32, 41, 413, 536
GlaI GCGC 3 cut(s) 318, 821, 823
GluI GCNGC 4 cut(s) 191, 305, 409, 813
HaeIII GGCC 5 cut(s) 144, 260, 411, 521, 621
HapII CCGG 2 cut(s) 207, 249
HhaI GCGC 3 cut(s) 319, 822, 824
Hin1I GRCGYC 1 cut(s) 354
Hin1II CATG 5 cut(s) 304, 352, 367, 587, 716
Hin6I GCGC 3 cut(s) 317, 820, 822
HinP1I GCGC 3 cut(s) 317, 820, 822
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HindIII AAGCTT 1 cut(s) 744
HinfI GANTC 1 cut(s) 173
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 2 cut(s) 207, 249
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 3 cut(s) 323, 475, 606
Hpy188I TCNGA 8 cut(s) 92, 133, 232, 283, 298, 529, 597, 808
Hpy188III TCNNGA 2 cut(s) 163, 713
Hpy8I GTNNAC 3 cut(s) 323, 475, 606
HpyAV CCTTC 3 cut(s) 118, 565, 638
HpyCH4IV ACGT 2 cut(s) 354, 390
HpyCH4V TGCA 7 cut(s) 193, 307, 367, 445, 671, 676, 686
HpyF10VI GCNNNNNNNGC 1 cut(s) 821
HpyF3I CTNAG 4 cut(s) 231, 282, 615, 756
HpySE526I ACGT 2 cut(s) 354, 390
Hsp92I GRCGYC 1 cut(s) 354
Hsp92II CATG 5 cut(s) 304, 352, 367, 587, 716
HspAI GCGC 3 cut(s) 317, 820, 822
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 3 cut(s) 177, 291, 799
LweI GCATC 5 cut(s) 235, 406, 651, 685, 799
MaeI CTAG 4 cut(s) 32, 41, 413, 536
MaeII ACGT 2 cut(s) 354, 390
MaeIII GTNAC 1 cut(s) 355
MalI GATC 1 cut(s) 37
MboI GATC 1 cut(s) 35
MboII GAAGA 1 cut(s) 684
MhlI GDGCHC 3 cut(s) 138, 207, 237
MluCI AATT 4 cut(s) 64, 500, 681, 765
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 7 cut(s) 37, 115, 248, 277, 367, 463, 603
MseI TTAA 2 cut(s) 255, 474
MslI CAYNNNNRTG 3 cut(s) 240, 312, 362
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 2 cut(s) 207, 249
MspR9I CCNGG 3 cut(s) 207, 330, 523
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 2 cut(s) 330, 523
MvnI CGCG 2 cut(s) 319, 822
MwoI GCNNNNNNNGC 1 cut(s) 821
NciI CCSGG 1 cut(s) 207
NdeII GATC 1 cut(s) 35
NlaIII CATG 5 cut(s) 304, 352, 367, 587, 716
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 1 cut(s) 355
NspI RCATGY 1 cut(s) 304
PagI TCATGA 1 cut(s) 712
PauI GCGCGC 1 cut(s) 820
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 294
PctI GAATGC 1 cut(s) 782
PflMI CCANNNNNTGG 1 cut(s) 437
PkrI GCNGC 4 cut(s) 192, 306, 410, 814
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 138
Psp6I CCWGG 2 cut(s) 328, 521
PspGI CCWGG 2 cut(s) 328, 521
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 5 cut(s) 129, 143, 427, 519, 619
PteI GCGCGC 1 cut(s) 820
RsaI GTAC 5 cut(s) 30, 119, 198, 217, 793
RsaNI GTAC 5 cut(s) 29, 118, 197, 216, 792
RseI CAYNNNNRTG 3 cut(s) 240, 312, 362
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 2 cut(s) 255, 474
SatI GCNGC 4 cut(s) 191, 305, 409, 813
Sau3AI GATC 1 cut(s) 35
Sau96I GGNCC 5 cut(s) 129, 143, 427, 519, 619
ScaI AGTACT 1 cut(s) 198
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 3 cut(s) 207, 330, 523
SduI GDGCHC 3 cut(s) 138, 207, 237
SfaNI GCATC 5 cut(s) 235, 406, 651, 685, 799
SfcI CTRYAG 1 cut(s) 727
SinI GGWCC 2 cut(s) 129, 427
SmiMI CAYNNNNRTG 3 cut(s) 240, 312, 362
Sse9I AATT 4 cut(s) 64, 500, 681, 765
SsiI CCGC 6 cut(s) 188, 213, 310, 408, 559, 801
SspI AATATT 1 cut(s) 461
SspMI CTAG 4 cut(s) 32, 41, 413, 536
SstI GAGCTC 1 cut(s) 138
StyD4I CCNGG 3 cut(s) 205, 328, 521
StyI CCWWGG 1 cut(s) 139
TaiI ACGT 2 cut(s) 357, 393
TaqI TCGA 2 cut(s) 111, 372
TasI AATT 4 cut(s) 64, 500, 681, 765
TatI WGTACW 1 cut(s) 196
TauI GCSGC 1 cut(s) 411
Tru1I TTAA 2 cut(s) 255, 474
Tru9I TTAA 2 cut(s) 255, 474
TscAI CASTG 3 cut(s) 153, 364, 678
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 3 cut(s) 190, 304, 812
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 4 cut(s) 67, 350, 572, 729
TspRI CASTG 3 cut(s) 153, 364, 678
Van91I CCANNNNNTGG 1 cut(s) 437
VpaK11BI GGWCC 2 cut(s) 129, 427
XapI RAATTY 2 cut(s) 500, 765
XceI RCATGY 1 cut(s) 304
XmiI GTMKAC 2 cut(s) 322, 605
XspI CTAG 4 cut(s) 32, 41, 413, 536
ZraI GACGTC 1 cut(s) 355
ZrmI AGTACT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.