MD04G1040500.v1.1

Serine threonine-protein kinase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016108
Physical Location & Seq
Reverse (-)
4748615 .. 4751353
2739 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1040500.v1.1.491

Sequence Viewer

Length: 2739 bp
ATGGGGTTTTCAGTAAATGATTTCCTGATCTGGGTTCTTAATATCAGCATACTGAGAGCTGGATTTCTTGCTGAACTTGTAGTTTTATCAGATTTATGGTGTTTAGCTTCAGGACTAGGTTCCATGTCAGCCATTGCAGTTTCTTATGGAGAGAAAGGTTCAGTTTTCTGTGGGTTAAAATTAGATGGGTCTCATCTTGTGACCTGCTATGGATCAAACTCAGCAATTACATACGGGGCTCCTAATCGATTCCCATTTATCGGTTTAACTGCTGGTGATGGATTTGTTTGTGGACTTCTCATGGATTCCAACCAGCCATATTGTTGGGGAAGCAGCGGTTATATCCAAATGGGTGTCCCTCAACCCATTGTTAAAGAAGCTCAATATTTAGAAATCAGTGCTGGTGATTATCATATGTGTGGATTACGAAAACCTTTAACTGGCAGGCTCAGAAACACCTCTTTTGTTGATTGTTGGGGATATAACATGACCAAAAACTATGTGTTTGATGGGCAGATTCAGTCGATTTCAGCTGGCTCAGAATTCAATTGTGGATTGTTTTCCCAGAACAGAACTGTCTTTTGCTGGGGTGATGAGACTAGTAGCAGGGTTATCAGCTTGATCCCCAAAGATATGAGGTTTCGGAAGATTTCAGCTGGTGGGTATCATGTTTGTGGGATTCTGGAGCCCGTGAATTCCAGAACCGTTTGTTGGGGAAGAAGCTTGGTCATGGAAGAAGAAATTTCAGTGGCATATTCAGGTCAGGGCAATGTTGATATGGCTCCAAATGACCCAATGCTTTCAGTTGTTGGAGGGAAGTTCCATGCTTGCGGAATTAAGAGCTATGACCGTGAGGTGATTTGTTGGGGGTTTATTGTGAAACAAAGTACACCTGCTCCTAATGGTACCAAGGTTTATGAGATTGCTGCTGGAAATTACTTCACTTGTGGAATTCTTGCTGACAAATCATTCTTACCGGTTTGCTGGGGACATGGATTCCCAACTTCTCTTCCATTACCTGTGGCACCTGGATTCTGCAAGTCCACTCCGTGCGCTCCTGGTTTCTATGAGCTCAGTAATGAGAGTGCCTCTTGCAAGTTACCTAACTCTCATATTTGCATGCCGTGCAGCAATGATTGTCCAGATGAAATGTACCAAAAAACCGAATGTACTTTGAAATCTGATAGACAGTGTGAATATAACTGTTCTATCTGTTACTCTGCTGAATGTTTCTCAAATTGTTCATCTTCATATGGCAAGAAGAATGAAAGGTTTTGGTCCATGCAATTGCCTATCATTATTGCGGAGGTTGCTTTTGCTCTGTTCTTGGTGAGTGTTGTGTCTATAACTGCAATTTTATATGTTCGCTACAGATTACGCAACTGTCAGTGCACAGGAAAAGATTTGAATTCGAAAAAGAATGGTCAGGATGCATCTTTCCAGAAGGACAATGGTAAGATTCGTCCCGAGTTGGATGACCTGAAGATAAGGAGAGCTCAGATGTTCACGTATGAGGAGCTTGAAAGGGCCACAGCCGGCTTTGGAGAGGAGTCTGTTGTTGGAAAGGGAAGCTTTTCCTGTGTTTTCAGAGGTGTTCTAAAAGATGGGACAGTTGTTGCTGTCAAAAAGGCTATAATGTCTCCTAACATGCAAAAGAATTCGAAAGAGTTCCATACAGAGCTAGACTTGCTCTCACGGTTAAACCACGCACATTTACTCAATCTGCTCGGATATTGTGAAGAAGGCGGACAAAGTCTTCTTGTTTACGAGTTCATGGCTCATGGATCATTGCATCAGCATCTCCACGGAAAGAACAAAGCCTTGAAAGAGCAATTGGATTGGGTTCGAAGGGTCACTATTGCAGTCCAAGCAGCTCGGGGAATTGAATACTTGCACGGTTATGCTTGCCCACCGGTGATTCATCGAGACATTAAGTCTTCAAACATTCTCATTGATGAGGAGCACAATGCTCGAGTTGCTGATTTTGGTTTGTCGTTACTGGGACCTGCAGACAGTGGCTCCCCATTGGCGGAGCTACCAGCCGGAACTCTAGGGTATCTTGATCCTGAGTACTACAGACTTCATTACCTTACAACCAAATCTGATGTTTACAGTTTTGGTGTTTTACTTTTGGAGATTCTTAGTGGCAGAAAAGCCATCGATATGCAGTACGAAGACGGAAATATAGTTGAATGGGCAGTGCCTCTGATCAAGTCAGGAGACATAAGTACAATTTTGGATCCAGTTTTGAAACCCCCACCTGACCTCGAAGCCTTGAAAAGAATTGCCAATGTAGCCTGCAAATGTGTGAGAATGAGAGGGAAGGAGAGGCCATCAATGGACAAGGTGACGACAGCGCTAGAACGAGCTCTTGCCATGCTAATGGGCAGCCCCTGTAACGAGCAACCTATTCTGCCAACCGAGGTGGTATTGGGAAGCAGTAGAATGCACAAAAAATCCTCACAAAGATCTTCAAACAGGTCAGTCGACACCGATGTGGTAGAACTGGAGGACGCGCAAAGGTTTGAATTCCGAGCTCCTTCATGGATCACATTTCCGAGTGTTGCTTCTTCCCAGAGGAGGAAGTCCTCGGCCTCAGATGCCGATGCCGATGGGAAGACCACAGAGGGTAGAAATTTGGGCAACTGTGGGAGTGGTGGTGATGGTTTGAGGAGCTTGGAAGAAGAGATTGGACCTGCATCACCTCAAGAGAAGTTATTCTTGAGGCATAATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009653 GO:0009786 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009986 GO:0009987 GO:0010008 GO:0010015 GO:0010016 GO:0010101 GO:0010102 GO:0010154 GO:0010311 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0030054 GO:0030139 GO:0030154 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0032501 GO:0032502 GO:0032585 GO:0036211 GO:0038023 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046983 GO:0048316 GO:0048364 GO:0048367 GO:0048439 GO:0048527 GO:0048528 GO:0048608 GO:0048646 GO:0048731 GO:0048829 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051302 GO:0051716 GO:0055044 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr RCC1_2 TNFR_c6

Protein Analysis

913

Amino Acids

100.32

Weight (kDa)

6.47

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 516 - 783 1.9e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 518 - 786 7.2e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59420
fragaria_vesca FvH4_4g10810
malus_domestica MD04G1040500.v1.1
prunus_persica Prupe.1G194300_v2.0.a1
pyrus_communis pycom04g03540
rosa_chinensis RchiOBHm_Chr4g0406691
rosa_laevigata RLG00000008760
rosa_multiflora Rmu_sc0017281.1_g000002
rosa_roxburghii Rroxscaffold_5G00351530
rosa_rugosa Rorug04G0072900
rosa_samantha Rh4AG140300 Rh4BG137300 Rh4CG147200 Rh4DG134300
rosa_wichuraiana Rw4G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 901
Acc36I ACCTGC 4 cut(s) 212, 901, 2014, 2704
Acc65I GGTACC 1 cut(s) 905
AccB1I GGYRCC 2 cut(s) 905, 1024
AccI GTMKAC 1 cut(s) 2487
AccII CGCG 1 cut(s) 2516
AciI CCGC 5 cut(s) 336, 831, 1304, 1746, 2030
AclWI GGATC 7 cut(s) 220, 616, 1792, 2057, 2234, 2247, 2555
AcsI RAATTY 8 cut(s) 542, 694, 741, 951, 1408, 1657, 2528, 2635
AcuI CTGAAG 2 cut(s) 93, 1502
AdeI CACNNNGTG 1 cut(s) 1050
AfaI GTAC 7 cut(s) 889, 907, 1154, 1171, 2072, 2171, 2230
AfeI AGCGCT 1 cut(s) 2358
AfiI CCNNNNNNNGG 6 cut(s) 31, 260, 440, 711, 2029, 2580
AgeI ACCGGT 2 cut(s) 976, 1912
AhdI GACNNNNNGTC 1 cut(s) 1933
AhlI ACTAGT 1 cut(s) 599
AjnI CCWGG 2 cut(s) 1027, 1057
AjuI GAANNNNNNNTTGG 6 cut(s) 694, 726, 1817, 1849, 2673, 2705
AleI CACNNNNGTG 1 cut(s) 2495
Alw21I GWGCWC 6 cut(s) 1074, 1394, 1498, 1965, 2371, 2539
Alw26I GTCTC 5 cut(s) 195, 590, 1644, 1920, 2214
Alw44I GTGCAC 1 cut(s) 1390
AlwI GGATC 7 cut(s) 220, 616, 1792, 2057, 2234, 2247, 2555
AlwNI CAGNNNCTG 1 cut(s) 2394
Ama87I CYCGRG 3 cut(s) 1466, 1875, 1971
Aor51HI AGCGCT 1 cut(s) 2358
AoxI GGCC 3 cut(s) 1527, 2330, 2592
ApaLI GTGCAC 1 cut(s) 1390
ApeKI GCWGC 5 cut(s) 333, 926, 1128, 1871, 2388
ApoI RAATTY 8 cut(s) 542, 694, 741, 951, 1408, 1657, 2528, 2635
AsiGI ACCGGT 2 cut(s) 976, 1912
Asp700I GAANNNNTTC 3 cut(s) 1573, 1667, 2717
Asp718I GGTACC 1 cut(s) 905
AspLEI GCGC 3 cut(s) 1055, 2359, 2518
AspS9I GGNCC 4 cut(s) 1278, 1527, 2003, 2693
AsuHPI GGTGA 9 cut(s) 287, 416, 602, 868, 1342, 1927, 2359, 2672, 2694
AsuII TTCGAA 3 cut(s) 1412, 1661, 1846
AvaI CYCGRG 3 cut(s) 1466, 1875, 1971
AvaII GGWCC 3 cut(s) 1278, 2003, 2693
BaeGI GKGCMC 1 cut(s) 1394
BaeI ACNNNNGTAYC 2 cut(s) 897, 930
BamHI GGATCC 1 cut(s) 2239
BanI GGYRCC 2 cut(s) 905, 1024
BanII GRGCYC 6 cut(s) 241, 690, 1074, 1498, 2371, 2539
BbsI GAAGAC 4 cut(s) 1748, 1929, 2181, 2624
Bbv12I GWGCWC 6 cut(s) 1074, 1394, 1498, 1965, 2371, 2539
BbvI GCAGC 5 cut(s) 345, 913, 1140, 1883, 2400
BccI CCATC 8 cut(s) 179, 272, 503, 1598, 2165, 2341, 2606, 2657
BceAI ACGGC 1 cut(s) 1108
BcgI CGANNNNNNTGC 2 cut(s) 1952, 1986
BciT130I CCWGG 2 cut(s) 1029, 1059
BclI TGATCA 1 cut(s) 2208
BcoDI GTCTC 5 cut(s) 195, 590, 1644, 1920, 2214
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 5 cut(s) 116, 600, 1682, 2051, 2360
BfmI CTRYAG 3 cut(s) 1369, 2007, 2074
BfoI RGCGCY 1 cut(s) 2360
BfuAI ACCTGC 4 cut(s) 212, 901, 2014, 2704
BglII AGATCT 1 cut(s) 2468
BisI GCNGC 5 cut(s) 334, 927, 1129, 1872, 2389
BlsI GCNGC 5 cut(s) 335, 928, 1130, 1873, 2390
BmcAI AGTACT 1 cut(s) 2072
Bme1390I CCNGG 2 cut(s) 1029, 1059
Bme18I GGWCC 3 cut(s) 1278, 2003, 2693
BmeRI GACNNNNNGTC 1 cut(s) 1933
BmeT110I CYCGRG 3 cut(s) 1466, 1875, 1971
BmgT120I GGNCC 4 cut(s) 1278, 1527, 2003, 2693
BmiI GGNNCC 9 cut(s) 121, 240, 687, 783, 907, 1026, 2004, 2020, 2241
BmrFI CCNGG 2 cut(s) 1029, 1059
BmrI ACTGGG 1 cut(s) 2009
BmsI GCATC 7 cut(s) 1420, 1442, 1801, 1807, 2590, 2596, 2708
BmuI ACTGGG 1 cut(s) 2009
BpiI GAAGAC 4 cut(s) 1748, 1929, 2181, 2624
BplI GAGNNNNNCTC 2 cut(s) 1073, 1105
BpmI CTGGAG 2 cut(s) 704, 2528
Bpu14I TTCGAA 3 cut(s) 1412, 1661, 1846
BpuEI CTTGAG 1 cut(s) 2691
Bsa29I ATCGAT 2 cut(s) 247, 2160
BsaAI YACGTR 1 cut(s) 1509
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 909, 1804, 2421, 2589
BsaWI WCCGGW 2 cut(s) 976, 1912
BsaXI ACNNNNNCTCC 6 cut(s) 880, 910, 2003, 2033, 2644, 2674
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 260, 440, 711, 2029, 2580
Bse118I RCCGGY 3 cut(s) 976, 1535, 1912
Bse1I ACTGG 4 cut(s) 445, 2004, 2243, 2511
Bse3DI GCAATG 4 cut(s) 132, 775, 1138, 1787
BseBI CCWGG 2 cut(s) 1029, 1059
BseCI ATCGAT 2 cut(s) 247, 2160
BseDI CCNNGG 4 cut(s) 909, 1804, 2421, 2589
BseGI GGATG 2 cut(s) 1435, 1480
BseLI CCNNNNNNNGG 6 cut(s) 31, 260, 440, 711, 2029, 2580
BseMI GCAATG 4 cut(s) 132, 775, 1138, 1787
BseMII CTCAG 8 cut(s) 44, 234, 463, 552, 1087, 1511, 2058, 2610
BseNI ACTGG 4 cut(s) 445, 2004, 2243, 2511
BseRI GAGGAG 5 cut(s) 1529, 1562, 1973, 2593, 2686
BseSI GKGCMC 1 cut(s) 1394
BseXI GCAGC 5 cut(s) 345, 913, 1140, 1883, 2400
BseYI CCCAGC 2 cut(s) 585, 984
BsgI GTGCAG 1 cut(s) 1147
Bsh1236I CGCG 1 cut(s) 2516
BshFI GGCC 3 cut(s) 1529, 2332, 2594
BshNI GGYRCC 2 cut(s) 905, 1024
BshTI ACCGGT 2 cut(s) 976, 1912
BshVI ATCGAT 2 cut(s) 247, 2160
BsiHKAI GWGCWC 6 cut(s) 1074, 1394, 1498, 1965, 2371, 2539
BsiHKCI CYCGRG 3 cut(s) 1466, 1875, 1971
BsiSI CCGG 4 cut(s) 977, 1536, 1913, 2043
BslFI GGGAC 5 cut(s) 341, 1002, 1449, 1621, 2016
BslI CCNNNNNNNGG 6 cut(s) 31, 260, 440, 711, 2029, 2580
BsmAI GTCTC 5 cut(s) 195, 590, 1644, 1920, 2214
BsmFI GGGAC 5 cut(s) 341, 1002, 1449, 1621, 2016
BsmI GAATGC 1 cut(s) 2451
BsnI GGCC 3 cut(s) 1529, 2332, 2594
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 3 cut(s) 1466, 1875, 1971
Bsp119I TTCGAA 3 cut(s) 1412, 1661, 1846
Bsp1286I GDGCHC 8 cut(s) 241, 690, 1074, 1394, 1498, 1965, 2371, 2539
Bsp143I GATC 9 cut(s) 27, 212, 621, 1784, 2062, 2208, 2239, 2468, 2547
BspACI CCGC 5 cut(s) 336, 831, 1304, 1746, 2030
BspANI GGCC 3 cut(s) 1529, 2332, 2594
BspCNI CTCAG 8 cut(s) 45, 233, 462, 551, 1086, 1510, 2059, 2609
BspDI ATCGAT 2 cut(s) 247, 2160
BspFNI CGCG 1 cut(s) 2516
BspLI GGNNCC 9 cut(s) 121, 240, 687, 783, 907, 1026, 2004, 2020, 2241
BspMAI CTGCAG 1 cut(s) 2011
BspMI ACCTGC 4 cut(s) 212, 901, 2014, 2704
BspPI GGATC 7 cut(s) 220, 616, 1792, 2057, 2234, 2247, 2555
BspT104I TTCGAA 3 cut(s) 1412, 1661, 1846
BspT107I GGYRCC 2 cut(s) 905, 1024
BspTNI GGTCTC 1 cut(s) 195
BsrDI GCAATG 4 cut(s) 132, 775, 1138, 1787
BsrFI RCCGGY 3 cut(s) 976, 1535, 1912
BsrI ACTGG 4 cut(s) 445, 2004, 2243, 2511
BssAI RCCGGY 3 cut(s) 976, 1535, 1912
BssECI CCNNGG 4 cut(s) 909, 1804, 2421, 2589
BssMI GATC 9 cut(s) 27, 212, 621, 1784, 2062, 2208, 2239, 2468, 2547
BssT1I CCWWGG 1 cut(s) 909
Bst2UI CCWGG 2 cut(s) 1029, 1059
Bst6I CTCTTC 2 cut(s) 1014, 2679
BstAPI GCANNNNNTGC 1 cut(s) 1125
BstBAI YACGTR 1 cut(s) 1509
BstBI TTCGAA 3 cut(s) 1412, 1661, 1846
BstC8I GCNNGC 7 cut(s) 446, 535, 829, 1121, 1537, 1906, 2299
BstDEI CTNAG 9 cut(s) 53, 220, 449, 538, 1073, 1497, 2067, 2141, 2596
BstDSI CCRYGG 1 cut(s) 1804
BstF5I GGATG 2 cut(s) 1435, 1480
BstFNI CGCG 1 cut(s) 2516
BstH2I RGCGCY 1 cut(s) 2360
BstHHI GCGC 3 cut(s) 1055, 2359, 2518
BstKTI GATC 9 cut(s) 30, 215, 624, 1787, 2065, 2211, 2242, 2471, 2550
BstMAI GTCTC 5 cut(s) 195, 590, 1644, 1920, 2214
BstMBI GATC 9 cut(s) 27, 212, 621, 1784, 2062, 2208, 2239, 2468, 2547
BstMWI GCNNNNNNNGC 7 cut(s) 1125, 1310, 1687, 1868, 1976, 2294, 2600
BstNI CCWGG 2 cut(s) 1029, 1059
BstNSI RCATGY 2 cut(s) 1123, 1651
BstSCI CCNGG 2 cut(s) 1027, 1057
BstSFI CTRYAG 3 cut(s) 1369, 2007, 2074
BstSLI GKGCMC 1 cut(s) 1394
BstUI CGCG 1 cut(s) 2516
BstV1I GCAGC 5 cut(s) 345, 913, 1140, 1883, 2400
BstV2I GAAGAC 4 cut(s) 1748, 1929, 2181, 2624
BstX2I RGATCY 2 cut(s) 2239, 2468
BstXI CCANNNNNNTGG 2 cut(s) 324, 2383
BstYI RGATCY 2 cut(s) 2239, 2468
Bsu15I ATCGAT 2 cut(s) 247, 2160
BsuRI GGCC 3 cut(s) 1529, 2332, 2594
BsuTUI ATCGAT 2 cut(s) 247, 2160
BtgI CCRYGG 1 cut(s) 1804
BtsCI GGATG 2 cut(s) 1435, 1480
BtsI GCAGTG 1 cut(s) 2205
BtsIMutI CAGTG 6 cut(s) 403, 753, 1196, 1394, 2020, 2205
BveI ACCTGC 4 cut(s) 212, 901, 2014, 2704
Cac8I GCNNGC 7 cut(s) 446, 535, 829, 1121, 1537, 1906, 2299
CaiI CAGNNNCTG 1 cut(s) 2394
CfoI GCGC 3 cut(s) 1055, 2359, 2518
Cfr10I RCCGGY 3 cut(s) 976, 1535, 1912
Cfr13I GGNCC 4 cut(s) 1278, 1527, 2003, 2693
ClaI ATCGAT 2 cut(s) 247, 2160
CseI GACGC 1 cut(s) 2522
Csp6I GTAC 7 cut(s) 888, 906, 1153, 1170, 2071, 2170, 2229
CspAI ACCGGT 2 cut(s) 976, 1912
CspCI CAANNNNNGTGG 2 cut(s) 2406, 2441
CviQI GTAC 7 cut(s) 888, 906, 1153, 1170, 2071, 2170, 2229
DdeI CTNAG 9 cut(s) 53, 220, 449, 538, 1073, 1497, 2067, 2141, 2596
DpnI GATC 9 cut(s) 29, 214, 623, 1786, 2064, 2210, 2241, 2470, 2549
DpnII GATC 9 cut(s) 27, 212, 621, 1784, 2062, 2208, 2239, 2468, 2547
DraIII CACNNNGTG 1 cut(s) 1050
DriI GACNNNNNGTC 1 cut(s) 1933
Eam1104I CTCTTC 2 cut(s) 1014, 2679
Eam1105I GACNNNNNGTC 1 cut(s) 1933
EarI CTCTTC 2 cut(s) 1014, 2679
EciI GGCGGA 2 cut(s) 1761, 2045
Ecl136II GAGCTC 4 cut(s) 1072, 1496, 2369, 2537
Eco130I CCWWGG 1 cut(s) 909
Eco24I GRGCYC 6 cut(s) 241, 690, 1074, 1498, 2371, 2539
Eco31I GGTCTC 1 cut(s) 195
Eco47I GGWCC 3 cut(s) 1278, 2003, 2693
Eco47III AGCGCT 1 cut(s) 2358
Eco53kI GAGCTC 4 cut(s) 1072, 1496, 2369, 2537
Eco57I CTGAAG 2 cut(s) 93, 1502
Eco88I CYCGRG 3 cut(s) 1466, 1875, 1971
EcoICRI GAGCTC 4 cut(s) 1072, 1496, 2369, 2537
EcoO109I RGGNCCY 1 cut(s) 2003
EcoRI GAATTC 6 cut(s) 542, 694, 951, 1408, 1657, 2528
EcoRII CCWGG 2 cut(s) 1027, 1057
EcoT14I CCWWGG 1 cut(s) 909
EcoT22I ATGCAT 1 cut(s) 1435
EcoT38I GRGCYC 6 cut(s) 241, 690, 1074, 1498, 2371, 2539
ErhI CCWWGG 1 cut(s) 909
FalI AAGNNNNNCTT 4 cut(s) 1556, 1588, 2705, 2737
FaqI GGGAC 5 cut(s) 341, 1002, 1449, 1621, 2016
FauNDI CATATG 2 cut(s) 414, 1252
FbaI TGATCA 1 cut(s) 2208
FblI GTMKAC 1 cut(s) 2487
Fnu4HI GCNGC 5 cut(s) 334, 927, 1129, 1872, 2389
FokI GGATG 2 cut(s) 1442, 1487
FriOI GRGCYC 6 cut(s) 241, 690, 1074, 1498, 2371, 2539
Fsp4HI GCNGC 5 cut(s) 334, 927, 1129, 1872, 2389
FspBI CTAG 5 cut(s) 116, 600, 1682, 2051, 2360
GlaI GCGC 3 cut(s) 1054, 2358, 2517
GluI GCNGC 5 cut(s) 334, 927, 1129, 1872, 2389
GsaI CCCAGC 2 cut(s) 589, 988
GsuI CTGGAG 2 cut(s) 704, 2528
HaeII RGCGCY 1 cut(s) 2360
HaeIII GGCC 3 cut(s) 1529, 2332, 2594
HapII CCGG 4 cut(s) 977, 1536, 1913, 2043
HgaI GACGC 1 cut(s) 2522
HhaI GCGC 3 cut(s) 1055, 2359, 2518
Hin6I GCGC 3 cut(s) 1053, 2357, 2516
HinP1I GCGC 3 cut(s) 1053, 2357, 2516
HincII GTYRAC 1 cut(s) 2488
HindII GTYRAC 1 cut(s) 2488
HindIII AAGCTT 2 cut(s) 721, 1570
HpaII CCGG 4 cut(s) 977, 1536, 1913, 2043
HphI GGTGA 9 cut(s) 287, 416, 602, 868, 1342, 1927, 2359, 2672, 2694
Hpy166II GTNNAC 8 cut(s) 293, 890, 1044, 1392, 1506, 1765, 2110, 2488
Hpy8I GTNNAC 8 cut(s) 293, 890, 1044, 1392, 1506, 1765, 2110, 2488
HpyAV CCTTC 5 cut(s) 1438, 1736, 1842, 2317, 2550
HpyCH4IV ACGT 1 cut(s) 1508
HpyF10VI GCNNNNNNNGC 7 cut(s) 1125, 1310, 1687, 1868, 1976, 2294, 2600
HpyF3I CTNAG 9 cut(s) 53, 220, 449, 538, 1073, 1497, 2067, 2141, 2596
HpySE526I ACGT 1 cut(s) 1508
HspAI GCGC 3 cut(s) 1053, 2357, 2516
KpnI GGTACC 1 cut(s) 909
KroI GCCGGC 1 cut(s) 1535
KroNI GCCGGC 1 cut(s) 1537
Ksp22I TGATCA 1 cut(s) 2208
Kzo9I GATC 9 cut(s) 27, 212, 621, 1784, 2062, 2208, 2239, 2468, 2547
Lsp1109I GCAGC 5 cut(s) 345, 913, 1140, 1883, 2400
LweI GCATC 7 cut(s) 1420, 1442, 1801, 1807, 2590, 2596, 2708
MaeI CTAG 5 cut(s) 116, 600, 1682, 2051, 2360
MaeII ACGT 1 cut(s) 1508
MaeIII GTNAC 7 cut(s) 199, 1098, 1214, 1852, 1995, 2347, 2396
MalI GATC 9 cut(s) 29, 214, 623, 1786, 2064, 2210, 2241, 2470, 2549
MboI GATC 9 cut(s) 27, 212, 621, 1784, 2062, 2208, 2239, 2468, 2547
MfeI CAATTG 3 cut(s) 547, 1286, 1832
MflI RGATCY 2 cut(s) 2239, 2468
MhlI GDGCHC 8 cut(s) 241, 690, 1074, 1394, 1498, 1965, 2371, 2539
MlyI GAGTC 1 cut(s) 1559
MmeI TCCRAC 4 cut(s) 333, 790, 1452, 1540
Mph1103I ATGCAT 1 cut(s) 1435
MroNI GCCGGC 1 cut(s) 1535
MroXI GAANNNNTTC 3 cut(s) 1573, 1667, 2717
MseI TTAA 9 cut(s) 39, 176, 266, 372, 437, 837, 1700, 1932, 2737
MslI CAYNNNNRTG 6 cut(s) 417, 672, 1899, 2162, 2381, 2495
MspA1I CMGCKG 3 cut(s) 336, 533, 656
MspI CCGG 4 cut(s) 977, 1536, 1913, 2043
MspR9I CCNGG 2 cut(s) 1029, 1059
MunI CAATTG 3 cut(s) 547, 1286, 1832
Mva1269I GAATGC 1 cut(s) 2451
MvaI CCWGG 2 cut(s) 1029, 1059
MvnI CGCG 1 cut(s) 2516
MwoI GCNNNNNNNGC 7 cut(s) 1125, 1310, 1687, 1868, 1976, 2294, 2600
NaeI GCCGGC 1 cut(s) 1537
NdeI CATATG 2 cut(s) 414, 1252
NdeII GATC 9 cut(s) 27, 212, 621, 1784, 2062, 2208, 2239, 2468, 2547
NgoMIV GCCGGC 1 cut(s) 1535
NlaIV GGNNCC 9 cut(s) 121, 240, 687, 783, 907, 1026, 2004, 2020, 2241
NmeAIII GCCGAG 1 cut(s) 2570
NmuCI GTSAC 3 cut(s) 199, 1852, 2347
NsiI ATGCAT 1 cut(s) 1435
NspI RCATGY 2 cut(s) 1123, 1651
NspV TTCGAA 3 cut(s) 1412, 1661, 1846
OliI CACNNNNGTG 1 cut(s) 2495
PaeI GCATGC 1 cut(s) 1123
PaeR7I CTCGAG 1 cut(s) 1971
PaqCI CACCTGC 1 cut(s) 901
PctI GAATGC 1 cut(s) 2451
PdiI GCCGGC 1 cut(s) 1537
PdmI GAANNNNTTC 3 cut(s) 1573, 1667, 2717
PfeI GAWTC 9 cut(s) 249, 305, 517, 679, 996, 1032, 1459, 1918, 2137
PflFI GACNNNGTC 1 cut(s) 1752
PinAI ACCGGT 2 cut(s) 976, 1912
PkrI GCNGC 5 cut(s) 335, 928, 1130, 1873, 2390
PleI GAGTC 1 cut(s) 1558
PpsI GAGTC 1 cut(s) 1558
Ppu21I YACGTR 1 cut(s) 1509
PpuMI RGGWCCY 1 cut(s) 2003
Psp124BI GAGCTC 4 cut(s) 1074, 1498, 2371, 2539
Psp5II RGGWCCY 1 cut(s) 2003
Psp6I CCWGG 2 cut(s) 1027, 1057
PspFI CCCAGC 2 cut(s) 585, 984
PspGI CCWGG 2 cut(s) 1027, 1057
PspN4I GGNNCC 9 cut(s) 121, 240, 687, 783, 907, 1026, 2004, 2020, 2241
PspPI GGNCC 4 cut(s) 1278, 1527, 2003, 2693
PspPPI RGGWCCY 1 cut(s) 2003
PspXI VCTCGAGB 1 cut(s) 1971
PstI CTGCAG 1 cut(s) 2011
PstNI CAGNNNCTG 1 cut(s) 2394
PsuI RGATCY 2 cut(s) 2239, 2468
PsyI GACNNNGTC 1 cut(s) 1752
PvuII CAGCTG 2 cut(s) 533, 656
RsaI GTAC 7 cut(s) 889, 907, 1154, 1171, 2072, 2171, 2230
RsaNI GTAC 7 cut(s) 888, 906, 1153, 1170, 2071, 2170, 2229
RseI CAYNNNNRTG 6 cut(s) 417, 672, 1899, 2162, 2381, 2495
SacI GAGCTC 4 cut(s) 1074, 1498, 2371, 2539
SalI GTCGAC 1 cut(s) 2486
SaqAI TTAA 9 cut(s) 39, 176, 266, 372, 437, 837, 1700, 1932, 2737
SatI GCNGC 5 cut(s) 334, 927, 1129, 1872, 2389
Sau3AI GATC 9 cut(s) 27, 212, 621, 1784, 2062, 2208, 2239, 2468, 2547
Sau96I GGNCC 4 cut(s) 1278, 1527, 2003, 2693
ScaI AGTACT 1 cut(s) 2072
SchI GAGTC 1 cut(s) 1559
ScrFI CCNGG 2 cut(s) 1029, 1059
SduI GDGCHC 8 cut(s) 241, 690, 1074, 1394, 1498, 1965, 2371, 2539
SfaNI GCATC 7 cut(s) 1420, 1442, 1801, 1807, 2590, 2596, 2708
SfcI CTRYAG 3 cut(s) 1369, 2007, 2074
Sfr274I CTCGAG 1 cut(s) 1971
SfuI TTCGAA 3 cut(s) 1412, 1661, 1846
SgrAI CRCCGGYG 1 cut(s) 1912
SinI GGWCC 3 cut(s) 1278, 2003, 2693
SlaI CTCGAG 1 cut(s) 1971
SmiMI CAYNNNNRTG 6 cut(s) 417, 672, 1899, 2162, 2381, 2495
SmlI CTYRAG 3 cut(s) 1971, 2706, 2722
SmoI CTYRAG 3 cut(s) 1971, 2706, 2722
SpeI ACTAGT 1 cut(s) 599
SphI GCATGC 1 cut(s) 1123
SsiI CCGC 5 cut(s) 336, 831, 1304, 1746, 2030
SspI AATATT 1 cut(s) 386
SspMI CTAG 5 cut(s) 116, 600, 1682, 2051, 2360
SstI GAGCTC 4 cut(s) 1074, 1498, 2371, 2539
StyD4I CCNGG 2 cut(s) 1027, 1057
StyI CCWWGG 1 cut(s) 909
TaiI ACGT 1 cut(s) 1511
TatI WGTACW 4 cut(s) 887, 1169, 2070, 2228
TfiI GAWTC 9 cut(s) 249, 305, 517, 679, 996, 1032, 1459, 1918, 2137
Tru1I TTAA 9 cut(s) 39, 176, 266, 372, 437, 837, 1700, 1932, 2737
Tru9I TTAA 9 cut(s) 39, 176, 266, 372, 437, 837, 1700, 1932, 2737
TscAI CASTG 6 cut(s) 403, 753, 1196, 1394, 2020, 2205
TseFI GTSAC 3 cut(s) 199, 1852, 2347
TseI GCWGC 5 cut(s) 333, 926, 1128, 1871, 2388
Tsp45I GTSAC 3 cut(s) 199, 1852, 2347
TspDTI ATGAA 8 cut(s) 1161, 1233, 1239, 1281, 1762, 1910, 2072, 2532
TspGWI ACGGA 3 cut(s) 1038, 1821, 2193
TspRI CASTG 6 cut(s) 403, 753, 1196, 1394, 2020, 2205
Tth111I GACNNNGTC 1 cut(s) 1752
VneI GTGCAC 1 cut(s) 1390
VpaK11BI GGWCC 3 cut(s) 1278, 2003, 2693
XapI RAATTY 8 cut(s) 542, 694, 741, 951, 1408, 1657, 2528, 2635
XceI RCATGY 2 cut(s) 1123, 1651
XcmI CCANNNNNNNNNTGG 1 cut(s) 1448
XhoI CTCGAG 1 cut(s) 1971
XmiI GTMKAC 1 cut(s) 2487
XmnI GAANNNNTTC 3 cut(s) 1573, 1667, 2717
XspI CTAG 5 cut(s) 116, 600, 1682, 2051, 2360
ZrmI AGTACT 1 cut(s) 2072
Zsp2I ATGCAT 1 cut(s) 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.