Rmu_sc0017281.1_g000002

Serine threonine-protein kinase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0017281.1
OG0016108
Physical Location & Seq
Reverse (-)
4371 .. 6989
2619 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0017281.1_g000002.1.cds

Sequence Viewer

Length: 2619 bp
atgtcctccgttgcagtttcttatggtgagaaaggtgcggtttactgtggcttaaacttgaatgggtctaatcttgtgagctgttatggatcaaactcggccataacatatggaaccccaattcacttcccatttattggtctgactgctggtgatggctttgtttgtggtctgctcatggattctaaccaaccatattgttggggcagcagtggttatattcaaatgggtgtccctcaacccattattaaagatgcccaatatgtagaaatcagtgctggtgattaccatctctgtggattgagaaaatctctaacagggagcctcagaaacatgtcttttgtcgattgttggggctataacatgaccaaaaactatgtgtttgatggtcagatgcagtccattacagcagggtcagaattcaattgtggattgttttcccaaaacaggactgtgttttgctggggtgatgagactagtagtagggttatccatttgatccccgcagacatgaggtttcggaagattgcagctggtgggtatcatgtctgtggcatttcagagggtgtgagttctagaaccttttgttggggaagaagcttggacattgaagaagaaatctcagtggcatattcgggtcaaggcaatgttgatttggctccgaaggacccaatgctttcaattgtgggagggaagtttcatgcctgcggaatcaagagctatgaccatggtgtcatctgctggggatttattatgaagccaagcacaccggttcccaagggtatcaaggtttatgacattgctgctggaaattacttcacttgtgggattcgtgctgacaaatcattcttgcctgtttgttggggtcttggatttccgacttctcttccattacctgttccccccaggtcctgcagctctactccatgtgctcctggcttctatgaactcagccatgacagcgcctcttgcaaagatcccaactctcacatttgcatgccctgcagcactggctgtcctgctgaaatgtaccaaaaaactgaatgtacgttgaaatctgatagacagtgtgattataactgttctatttgttcctcagctggatgctcctcaaactgttcttcattgtacgccaattcaaagaggaacgaaaggttctggtctctgcaattacctgtcattattgctgagattgcctttgctatgatattggtcagtgttgtgtctctaactgcagttttatatgtccgctacaagttacgtgactgtcgttgcacagaaaaatacttaaagtctacaaagaatggtcgcagtggttctccttaccagaaagacattggcaagattcgtccagacttggatgatctgaagataaggcgggctcagatgtttacttatgaggaactggaaagagcaacttcggggtttgaagaagagtctgttgtgggcaagggaagcttctcctcagtttttagaggtgttttgagagatggaacagttgtggctgtcaaaagggctataatgtctcctaacatgcaaaagaattcaaaagagttccatactgagctagacttgctctccagattaaaccatgctcatttactcaacctgcttggctattgtgaagaaggtggagagaggcttcttgtgtacgagttcatggctcatggatcattgcaccagcacctccatggaaagaacaaagccttgagagagcaattggactgggttcgaagagtcacaattgcagtccaggcagctaggggaattgaatacttgcatgggtacgcttgcccaccagtgatccatcgagatattaagtcttctaatatcttgatcgatgaggaacacaatgctagagtagctgattttggcttgtcactattgggacctgcagatagtagttccccattggccgagctaccagcgggaactctagggtatcttgatcctgagtactacaggcttcactacctgacaaccaaatctgatgtttatagttttggtgttatgctgttggagattcttagtggcagaaaagccattgatatgcaatatgaagaatcaggaaatatagttgaatgggcagtgcctctgatcaaggcaggggacatatttacacttttggatccagttttgaaacccccacctgatgttgaagccttgaaaagaattgcaaatgttgcttgcaaatgtgtgaggatgagaggcaaggagaggccgtcaatggataaagtgactacagcacttgaacgagctcttgcgctgctaatgggcagcccctgtaatgagcaacccattctgcctactgaggtggtattgggaagcagtagattgcacaagaagtcatcgcagagatcttcaaacaggtcagtggaaactgatgttatagatgccgaggatcagaggtttgaattcagagctccttcatggatcacatttccgagtgttgcttcttcccagaggaggaaatcctcggtctcagatgccgatgctgatgggaagagctccatggaagctagaaatatggggaatggtggtgatgggttgaggagtttggaggaagagattgggccagcttcacctcgagagaagctattcttgcagcataatttttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009653 GO:0009786 GO:0009790 GO:0009791 GO:0009793 GO:0009908 GO:0009986 GO:0009987 GO:0010008 GO:0010015 GO:0010016 GO:0010101 GO:0010102 GO:0010154 GO:0010311 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0022414 GO:0022622 GO:0023052 GO:0030054 GO:0030139 GO:0030154 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0032501 GO:0032502 GO:0032585 GO:0036211 GO:0038023 GO:0042802 GO:0042803 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0046777 GO:0046983 GO:0048316 GO:0048364 GO:0048367 GO:0048439 GO:0048527 GO:0048528 GO:0048608 GO:0048646 GO:0048731 GO:0048829 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051302 GO:0051716 GO:0055044 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0090567 GO:0090627 GO:0090696 GO:0090697 GO:0090698 GO:0097708 GO:0098588 GO:0098805 GO:0099402 GO:0140096 GO:1901564 GO:1905392 GO:1905393
KEGG Pathways
Metabolic & Signaling
ko04010 ko04064 ko04620 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05169 map04010 map04064 map04620 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05169
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr RCC1_2 TNFR_c6

Protein Analysis

872

Amino Acids

96.01

Weight (kDa)

7.02

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59420
fragaria_vesca FvH4_4g10810
malus_domestica MD04G1040500.v1.1
prunus_persica Prupe.1G194300_v2.0.a1
pyrus_communis pycom04g03540
rosa_chinensis RchiOBHm_Chr4g0406691
rosa_laevigata RLG00000008760
rosa_multiflora Rmu_sc0017281.1_g000002
rosa_roxburghii Rroxscaffold_5G00351530
rosa_rugosa Rorug04G0072900
rosa_samantha Rh4AG140300 Rh4BG137300 Rh4CG147200 Rh4DG134300
rosa_wichuraiana Rw4G011430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1077
AasI GACNNNNNNGTC 1 cut(s) 731
Acc36I ACCTGC 2 cut(s) 1617, 1900
AccB7I CCANNNNNTGG 1 cut(s) 137
AccI GTMKAC 1 cut(s) 1295
AciI CCGC 6 cut(s) 38, 504, 708, 1249, 1378, 1928
AcoI YGGCCR 2 cut(s) 99, 1914
AcsI RAATTY 3 cut(s) 419, 1543, 2414
AcuI CTGAAG 1 cut(s) 1388
AfaI GTAC 6 cut(s) 1031, 1048, 1130, 1652, 1787, 1958
AfiI CCNNNNNNNGG 4 cut(s) 137, 447, 588, 2466
AflIII ACRYGT 1 cut(s) 333
AgeI ACCGGT 1 cut(s) 769
AhlI ACTAGT 1 cut(s) 476
AjnI CCWGG 3 cut(s) 905, 934, 1752
AjuI GAANNNNNNNTTGG 2 cut(s) 571, 603
Alw21I GWGCWC 4 cut(s) 934, 2260, 2425, 2510
Alw26I GTCTC 5 cut(s) 467, 1167, 1230, 1530, 2485
AlwNI CAGNNNCTG 2 cut(s) 912, 2283
Ama87I CYCGRG 1 cut(s) 2586
AoxI GGCC 4 cut(s) 99, 1914, 2219, 2573
ApeKI GCWGC 9 cut(s) 207, 530, 803, 915, 1005, 1757, 2266, 2277, 2605
ApoI RAATTY 3 cut(s) 419, 1543, 2414
AsiGI ACCGGT 1 cut(s) 769
Asp700I GAANNNNTTC 1 cut(s) 2597
AspLEI GCGC 2 cut(s) 965, 2266
AspS9I GGNCC 4 cut(s) 667, 909, 1889, 2573
AsuHPI GGTGA 6 cut(s) 38, 164, 293, 479, 2552, 2574
AsuII TTCGAA 1 cut(s) 1732
AvaI CYCGRG 1 cut(s) 2586
AvaII GGWCC 3 cut(s) 667, 909, 1889
BamHI GGATCC 1 cut(s) 2128
BanII GRGCYC 4 cut(s) 1384, 2260, 2425, 2510
BbsI GAAGAC 1 cut(s) 1815
Bbv12I GWGCWC 4 cut(s) 934, 2260, 2425, 2510
BbvCI CCTCAGC 1 cut(s) 1096
BbvI GCAGC 8 cut(s) 219, 542, 790, 927, 1017, 1769, 2253, 2289
BccI CCATC 7 cut(s) 149, 297, 380, 1484, 1815, 2492, 2537
BceAI ACGGC 1 cut(s) 2206
BciT130I CCWGG 3 cut(s) 907, 936, 1754
BclI TGATCA 1 cut(s) 2097
BcoDI GTCTC 5 cut(s) 467, 1167, 1230, 1530, 2485
BcuI ACTAGT 1 cut(s) 476
BfaI CTAG 7 cut(s) 477, 576, 1568, 1761, 1857, 1937, 2520
BfmI CTRYAG 6 cut(s) 913, 1003, 1233, 1893, 1960, 2241
BfoI RGCGCY 1 cut(s) 966
BfuAI ACCTGC 2 cut(s) 1617, 1900
BglII AGATCT 1 cut(s) 2357
BisI GCNGC 9 cut(s) 208, 531, 804, 916, 1006, 1758, 2267, 2278, 2606
BlsI GCNGC 9 cut(s) 209, 532, 805, 917, 1007, 1759, 2268, 2279, 2607
BmcAI AGTACT 1 cut(s) 1958
Bme1390I CCNGG 3 cut(s) 907, 936, 1754
Bme18I GGWCC 3 cut(s) 667, 909, 1889
BmeT110I CYCGRG 1 cut(s) 2586
BmgT120I GGNCC 4 cut(s) 667, 909, 1889, 2573
BmiI GGNNCC 7 cut(s) 115, 323, 660, 669, 774, 1890, 2130
BmrFI CCNGG 3 cut(s) 907, 936, 1754
BmrI ACTGGG 1 cut(s) 1735
BmsI GCATC 6 cut(s) 244, 384, 1094, 2383, 2476, 2482
BmuI ACTGGG 1 cut(s) 1735
BpiI GAAGAC 1 cut(s) 1815
BplI GAGNNNNNCTC 2 cut(s) 295, 327
BpmI CTGGAG 1 cut(s) 1564
Bpu10I CCTNAGC 1 cut(s) 1096
Bpu14I TTCGAA 1 cut(s) 1732
BpuEI CTTGAG 1 cut(s) 1729
Bsa29I ATCGAT 1 cut(s) 1839
BsaAI YACGTR 1 cut(s) 1262
BsaBI GATNNNNATC 1 cut(s) 288
BsaI GGTCTC 2 cut(s) 1167, 2485
BsaJI CCNNGG 7 cut(s) 727, 777, 905, 1690, 2397, 2475, 2511
BsaWI WCCGGW 1 cut(s) 769
BsaXI ACNNNNNCTCC 4 cut(s) 1562, 1592, 2207, 2237
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 447, 588, 2466
Bse118I RCCGGY 1 cut(s) 769
Bse1I ACTGG 5 cut(s) 1015, 1410, 1730, 1799, 2132
Bse3DI GCAATG 3 cut(s) 652, 798, 1673
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 3 cut(s) 907, 936, 1754
BseCI ATCGAT 1 cut(s) 1839
BseDI CCNNGG 7 cut(s) 727, 777, 905, 1690, 2397, 2475, 2511
BseGI GGATG 3 cut(s) 1109, 1366, 2208
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 4 cut(s) 137, 447, 588, 2466
BseMI GCAATG 3 cut(s) 652, 798, 1673
BseNI ACTGG 5 cut(s) 1015, 1410, 1730, 1799, 2132
BseRI GAGGAG 4 cut(s) 1099, 1453, 2479, 2566
BseXI GCAGC 8 cut(s) 219, 542, 790, 927, 1017, 1769, 2253, 2289
BseYI CCCAGC 2 cut(s) 462, 741
BshFI GGCC 4 cut(s) 101, 1916, 2221, 2575
BshTI ACCGGT 1 cut(s) 769
BshVI ATCGAT 1 cut(s) 1839
BsiHKAI GWGCWC 4 cut(s) 934, 2260, 2425, 2510
BsiHKCI CYCGRG 1 cut(s) 2586
BsiSI CCGG 1 cut(s) 770
BslFI GGGAC 3 cut(s) 218, 1902, 2123
BslI CCNNNNNNNGG 4 cut(s) 137, 447, 588, 2466
BsmAI GTCTC 5 cut(s) 467, 1167, 1230, 1530, 2485
BsmFI GGGAC 3 cut(s) 218, 1902, 2123
BsnI GGCC 4 cut(s) 101, 1916, 2221, 2575
Bso31I GGTCTC 2 cut(s) 1167, 2485
BsoBI CYCGRG 1 cut(s) 2586
Bsp119I TTCGAA 1 cut(s) 1732
Bsp1286I GDGCHC 5 cut(s) 934, 1384, 2260, 2425, 2510
Bsp19I CCATGG 3 cut(s) 727, 1690, 2511
BspACI CCGC 6 cut(s) 38, 504, 708, 1249, 1378, 1928
BspANI GGCC 4 cut(s) 101, 1916, 2221, 2575
BspDI ATCGAT 1 cut(s) 1839
BspLI GGNNCC 7 cut(s) 115, 323, 660, 669, 774, 1890, 2130
BspMAI CTGCAG 4 cut(s) 917, 1007, 1237, 1897
BspMI ACCTGC 2 cut(s) 1617, 1900
BspQI GCTCTTC 1 cut(s) 2498
BspT104I TTCGAA 1 cut(s) 1732
BspTNI GGTCTC 2 cut(s) 1167, 2485
BsrDI GCAATG 3 cut(s) 652, 798, 1673
BsrFI RCCGGY 1 cut(s) 769
BsrI ACTGG 5 cut(s) 1015, 1410, 1730, 1799, 2132
BssAI RCCGGY 1 cut(s) 769
BssECI CCNNGG 7 cut(s) 727, 777, 905, 1690, 2397, 2475, 2511
BssT1I CCWWGG 4 cut(s) 727, 777, 1690, 2511
Bst2UI CCWGG 3 cut(s) 907, 936, 1754
Bst4CI ACNGT 7 cut(s) 47, 454, 1068, 1082, 1118, 1268, 1498
Bst6I CTCTTC 5 cut(s) 891, 1428, 1729, 2498, 2559
BstAPI GCANNNNNTGC 2 cut(s) 1002, 2183
BstBAI YACGTR 1 cut(s) 1262
BstBI TTCGAA 1 cut(s) 1732
BstC8I GCNNGC 6 cut(s) 706, 998, 1380, 1792, 2188, 2577
BstDSI CCRYGG 3 cut(s) 727, 1690, 2511
BstF5I GGATG 3 cut(s) 1109, 1366, 2208
BstH2I RGCGCY 1 cut(s) 966
BstHHI GCGC 2 cut(s) 965, 2266
BstMAI GTCTC 5 cut(s) 467, 1167, 1230, 1530, 2485
BstNI CCWGG 3 cut(s) 907, 936, 1754
BstNSI RCATGY 3 cut(s) 337, 1000, 1537
BstSCI CCNGG 3 cut(s) 905, 934, 1752
BstSFI CTRYAG 6 cut(s) 913, 1003, 1233, 1893, 1960, 2241
BstV1I GCAGC 8 cut(s) 219, 542, 790, 927, 1017, 1769, 2253, 2289
BstV2I GAAGAC 1 cut(s) 1815
BstX2I RGATCY 3 cut(s) 976, 2128, 2357
BstXI CCANNNNNNTGG 2 cut(s) 201, 296
BstYI RGATCY 3 cut(s) 976, 2128, 2357
Bsu15I ATCGAT 1 cut(s) 1839
BsuRI GGCC 4 cut(s) 101, 1916, 2221, 2575
BsuTUI ATCGAT 1 cut(s) 1839
BtgI CCRYGG 3 cut(s) 727, 1690, 2511
BtgZI GCGATG 1 cut(s) 2334
BtsCI GGATG 3 cut(s) 1109, 1366, 2208
BtsI GCAGTG 3 cut(s) 217, 1318, 2094
BveI ACCTGC 2 cut(s) 1617, 1900
Cac8I GCNNGC 6 cut(s) 706, 998, 1380, 1792, 2188, 2577
CaiI CAGNNNCTG 2 cut(s) 912, 2283
CfoI GCGC 2 cut(s) 965, 2266
Cfr10I RCCGGY 1 cut(s) 769
Cfr13I GGNCC 4 cut(s) 667, 909, 1889, 2573
ClaI ATCGAT 1 cut(s) 1839
Csp6I GTAC 6 cut(s) 1030, 1047, 1129, 1651, 1786, 1957
CspAI ACCGGT 1 cut(s) 769
CviQI GTAC 6 cut(s) 1030, 1047, 1129, 1651, 1786, 1957
DrdI GACNNNNNNGTC 1 cut(s) 731
DseDI GACNNNNNNGTC 1 cut(s) 731
EaeI YGGCCR 2 cut(s) 99, 1914
Eam1104I CTCTTC 5 cut(s) 891, 1428, 1729, 2498, 2559
EarI CTCTTC 5 cut(s) 891, 1428, 1729, 2498, 2559
Ecl136II GAGCTC 3 cut(s) 2258, 2423, 2508
Eco130I CCWWGG 4 cut(s) 727, 777, 1690, 2511
Eco24I GRGCYC 4 cut(s) 1384, 2260, 2425, 2510
Eco31I GGTCTC 2 cut(s) 1167, 2485
Eco47I GGWCC 3 cut(s) 667, 909, 1889
Eco53kI GAGCTC 3 cut(s) 2258, 2423, 2508
Eco57I CTGAAG 1 cut(s) 1388
Eco88I CYCGRG 1 cut(s) 2586
EcoICRI GAGCTC 3 cut(s) 2258, 2423, 2508
EcoO109I RGGNCCY 3 cut(s) 667, 909, 1889
EcoRI GAATTC 3 cut(s) 419, 1543, 2414
EcoRII CCWGG 3 cut(s) 905, 934, 1752
EcoT14I CCWWGG 4 cut(s) 727, 777, 1690, 2511
EcoT38I GRGCYC 4 cut(s) 1384, 2260, 2425, 2510
ErhI CCWWGG 4 cut(s) 727, 777, 1690, 2511
FalI AAGNNNNNCTT 6 cut(s) 1402, 1434, 1442, 1474, 2585, 2617
FaqI GGGAC 3 cut(s) 218, 1902, 2123
FauI CCCGC 3 cut(s) 511, 1371, 1921
FauNDI CATATG 1 cut(s) 109
FbaI TGATCA 1 cut(s) 2097
FblI GTMKAC 1 cut(s) 1295
Fnu4HI GCNGC 9 cut(s) 208, 531, 804, 916, 1006, 1758, 2267, 2278, 2606
FokI GGATG 3 cut(s) 1116, 1373, 2215
FriOI GRGCYC 4 cut(s) 1384, 2260, 2425, 2510
Fsp4HI GCNGC 9 cut(s) 208, 531, 804, 916, 1006, 1758, 2267, 2278, 2606
FspBI CTAG 7 cut(s) 477, 576, 1568, 1761, 1857, 1937, 2520
GlaI GCGC 2 cut(s) 964, 2265
GluI GCNGC 9 cut(s) 208, 531, 804, 916, 1006, 1758, 2267, 2278, 2606
GsaI CCCAGC 2 cut(s) 466, 745
GsuI CTGGAG 1 cut(s) 1564
HaeII RGCGCY 1 cut(s) 966
HaeIII GGCC 4 cut(s) 101, 1916, 2221, 2575
HapII CCGG 1 cut(s) 770
HhaI GCGC 2 cut(s) 965, 2266
Hin6I GCGC 2 cut(s) 963, 2264
HinP1I GCGC 2 cut(s) 963, 2264
HindIII AAGCTT 2 cut(s) 598, 1456
HinfI GANTC 8 cut(s) 182, 711, 829, 1345, 1436, 1737, 2023, 2063
HpaII CCGG 1 cut(s) 770
HphI GGTGA 6 cut(s) 38, 164, 293, 479, 2552, 2574
Hpy166II GTNNAC 4 cut(s) 43, 1296, 1392, 1651
Hpy8I GTNNAC 4 cut(s) 43, 1296, 1392, 1651
HpyAV CCTTC 3 cut(s) 658, 1622, 2436
HpyCH4III ACNGT 7 cut(s) 47, 454, 1068, 1082, 1118, 1268, 1498
HpyCH4IV ACGT 2 cut(s) 1049, 1261
HpySE526I ACGT 2 cut(s) 1049, 1261
HspAI GCGC 2 cut(s) 963, 2264
Ksp22I TGATCA 1 cut(s) 2097
LguI GCTCTTC 1 cut(s) 2498
LmnI GCTCC 6 cut(s) 321, 664, 937, 1112, 2428, 2513
Lsp1109I GCAGC 8 cut(s) 219, 542, 790, 927, 1017, 1769, 2253, 2289
LweI GCATC 6 cut(s) 244, 384, 1094, 2383, 2476, 2482
MaeI CTAG 7 cut(s) 477, 576, 1568, 1761, 1857, 1937, 2520
MaeII ACGT 2 cut(s) 1049, 1261
MaeIII GTNAC 5 cut(s) 1257, 1262, 1738, 1878, 2236
MfeI CAATTG 4 cut(s) 424, 681, 1718, 1743
MflI RGATCY 3 cut(s) 976, 2128, 2357
MhlI GDGCHC 5 cut(s) 934, 1384, 2260, 2425, 2510
MlyI GAGTC 2 cut(s) 1445, 1746
MmeI TCCRAC 2 cut(s) 902, 1998
MroXI GAANNNNTTC 1 cut(s) 2597
MseI TTAA 6 cut(s) 53, 249, 1289, 1586, 1818, 2617
MslI CAYNNNNRTG 5 cut(s) 294, 549, 995, 1689, 2048
MspA1I CMGCKG 3 cut(s) 533, 1100, 1928
MspI CCGG 1 cut(s) 770
MspR9I CCNGG 3 cut(s) 907, 936, 1754
MunI CAATTG 4 cut(s) 424, 681, 1718, 1743
MvaI CCWGG 3 cut(s) 907, 936, 1754
NcoI CCATGG 3 cut(s) 727, 1690, 2511
NdeI CATATG 1 cut(s) 109
NlaIV GGNNCC 7 cut(s) 115, 323, 660, 669, 774, 1890, 2130
NmeAIII GCCGAG 3 cut(s) 77, 1942, 2422
NmuCI GTSAC 4 cut(s) 1262, 1738, 1878, 2236
NspI RCATGY 3 cut(s) 337, 1000, 1537
NspV TTCGAA 1 cut(s) 1732
PaeI GCATGC 1 cut(s) 1000
PaeR7I CTCGAG 1 cut(s) 2586
PciI ACATGT 1 cut(s) 333
PciSI GCTCTTC 1 cut(s) 2498
PcsI WCGNNNNNNNCGW 1 cut(s) 1267
PdmI GAANNNNTTC 1 cut(s) 2597
PfeI GAWTC 6 cut(s) 182, 711, 829, 1345, 2023, 2063
PflMI CCANNNNNTGG 1 cut(s) 137
PinAI ACCGGT 1 cut(s) 769
PkrI GCNGC 9 cut(s) 209, 532, 805, 917, 1007, 1759, 2268, 2279, 2607
PleI GAGTC 2 cut(s) 1444, 1745
PpsI GAGTC 2 cut(s) 1444, 1745
Ppu21I YACGTR 1 cut(s) 1262
PpuMI RGGWCCY 3 cut(s) 667, 909, 1889
PscI ACATGT 1 cut(s) 333
PsiI TTATAA 1 cut(s) 1077
Psp124BI GAGCTC 3 cut(s) 2260, 2425, 2510
Psp5II RGGWCCY 3 cut(s) 667, 909, 1889
Psp6I CCWGG 3 cut(s) 905, 934, 1752
PspFI CCCAGC 2 cut(s) 462, 741
PspGI CCWGG 3 cut(s) 905, 934, 1752
PspN4I GGNNCC 7 cut(s) 115, 323, 660, 669, 774, 1890, 2130
PspPI GGNCC 4 cut(s) 667, 909, 1889, 2573
PspPPI RGGWCCY 3 cut(s) 667, 909, 1889
PstI CTGCAG 4 cut(s) 917, 1007, 1237, 1897
PstNI CAGNNNCTG 2 cut(s) 912, 2283
PsuI RGATCY 3 cut(s) 976, 2128, 2357
PvuII CAGCTG 2 cut(s) 533, 1100
RsaI GTAC 6 cut(s) 1031, 1048, 1130, 1652, 1787, 1958
RsaNI GTAC 6 cut(s) 1030, 1047, 1129, 1651, 1786, 1957
RseI CAYNNNNRTG 5 cut(s) 294, 549, 995, 1689, 2048
SacI GAGCTC 3 cut(s) 2260, 2425, 2510
SapI GCTCTTC 1 cut(s) 2498
SaqAI TTAA 6 cut(s) 53, 249, 1289, 1586, 1818, 2617
SatI GCNGC 9 cut(s) 208, 531, 804, 916, 1006, 1758, 2267, 2278, 2606
Sau96I GGNCC 4 cut(s) 667, 909, 1889, 2573
ScaI AGTACT 1 cut(s) 1958
SchI GAGTC 2 cut(s) 1445, 1746
ScrFI CCNGG 3 cut(s) 907, 936, 1754
SduI GDGCHC 5 cut(s) 934, 1384, 2260, 2425, 2510
SfaNI GCATC 6 cut(s) 244, 384, 1094, 2383, 2476, 2482
SfcI CTRYAG 6 cut(s) 913, 1003, 1233, 1893, 1960, 2241
Sfr274I CTCGAG 1 cut(s) 2586
SfuI TTCGAA 1 cut(s) 1732
SinI GGWCC 3 cut(s) 667, 909, 1889
SlaI CTCGAG 1 cut(s) 2586
SmiMI CAYNNNNRTG 5 cut(s) 294, 549, 995, 1689, 2048
SmlI CTYRAG 2 cut(s) 1708, 2586
SmoI CTYRAG 2 cut(s) 1708, 2586
SpeI ACTAGT 1 cut(s) 476
SphI GCATGC 1 cut(s) 1000
SsiI CCGC 6 cut(s) 38, 504, 708, 1249, 1378, 1928
SspMI CTAG 7 cut(s) 477, 576, 1568, 1761, 1857, 1937, 2520
SstI GAGCTC 3 cut(s) 2260, 2425, 2510
StyD4I CCNGG 3 cut(s) 905, 934, 1752
StyI CCWWGG 4 cut(s) 727, 777, 1690, 2511
TaaI ACNGT 7 cut(s) 47, 454, 1068, 1082, 1118, 1268, 1498
TaiI ACGT 2 cut(s) 1052, 1264
TaqI TCGA 5 cut(s) 345, 1732, 1810, 1839, 2587
TaqII GACCGA 1 cut(s) 2467
TatI WGTACW 1 cut(s) 1956
TfiI GAWTC 6 cut(s) 182, 711, 829, 1345, 2023, 2063
Tru1I TTAA 6 cut(s) 53, 249, 1289, 1586, 1818, 2617
Tru9I TTAA 6 cut(s) 53, 249, 1289, 1586, 1818, 2617
TseFI GTSAC 4 cut(s) 1262, 1738, 1878, 2236
TseI GCWGC 9 cut(s) 207, 530, 803, 915, 1005, 1757, 2266, 2277, 2605
Tsp45I GTSAC 4 cut(s) 1262, 1738, 1878, 2236
TspDTI ATGAA 7 cut(s) 689, 770, 960, 1113, 1648, 2073, 2418
Van91I CCANNNNNTGG 1 cut(s) 137
VpaK11BI GGWCC 3 cut(s) 667, 909, 1889
XapI RAATTY 3 cut(s) 419, 1543, 2414
XbaI TCTAGA 1 cut(s) 575
XceI RCATGY 3 cut(s) 337, 1000, 1537
XcmI CCANNNNNNNNNTGG 2 cut(s) 1334, 1688
XhoI CTCGAG 1 cut(s) 2586
XmiI GTMKAC 1 cut(s) 1295
XmnI GAANNNNTTC 1 cut(s) 2597
XspI CTAG 7 cut(s) 477, 576, 1568, 1761, 1857, 1937, 2520
ZrmI AGTACT 1 cut(s) 1958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.