Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006182

Physical Location & Seq

Genomic Coordinates Forward (+)

5784950 .. 5790524

Gene Structure 5575 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1049400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1728 bp

ATGGGAAAGAAAGGGAGTTGGTTTTCCGCCATAAGGAGGGTTTTCTCTCCTCATTCCAAGGACAAGGACAAGGACAAGGCAGTCAATGGTTCGGAGAGGAAAAACACAAAAGAAAAGAAGAAGGGGCTTGGGAAACTAAATCGTGGAGAGACCAATTCATTCATTCCGCTGTTCAGAGAACCAAGCAGCGTTGAGAAAATTTTTGGGGATTTTGAGAGGGAGCAACAAAGAGTAACTTTTAGGCCTCCTACACCTGATGAGCAACCAAAGACCCCACCATTTGTGCCTCCTAGAGTTGCTTCTCCGAGGGTTGGTTCACCAAGGGCTGCTTCTCCAAGGTCTTCTTCCCCAAGAGCTCCTTCTCCAAGGGCTCTTTCCCCAAGAGCTCCTTCTCCAAGGGCTCTTTCCCCAAGAGCTCCACCTCCAAGAGCTCCCCCTCCTCCAAGAGCTCCTCCTCCAAGAGCTCCTCCTCCAAGAGCTCAATCTCCTAGCACCTTTCATCAACATAAGGAGATTAGCTACAGACCTGAACCAACTCTAAGGAACCACCATGCCTCAGCTACTAAAATTCAGGCAGTCTATAGAGGTTATATGGCAAGAAGAAGCTTTAGAGCTCTAAAGGGTCTAGTGAGGCTTCAAGGAGTAGTAAGAGGCCAGAACGTGAAGCGTCAGACGATGAATGCCATGAAATACATGCAACTCTCGGTGCGTGTTCAGTCTCAAATTCAGTCACGGAGGATTCAGATGTTAGAAAACCAAGCACGACATCAAGTCCAAAACAAGTATGACAAAGATGTGGAAAGCACCTTCGGCAAATGGAACTTGAGCCAGCAATCTGAGACGGCCAATAACGAGGAATGGGATGATAGCTTGCTAACTAAGGAGGAAGTGGAGGCAAGGTTGCAGAAAAAGGTTGAGGCAGTCATGAAAAGAGAGAGAGCAATGGCTTACGCATACTCCAACCAGCTGTGGAAGGCTACTCCTAAATCAGGTCAAACACCTATAGCTGATATCCGGTCTGGGGGATTTCCGTGGTGGTGGAACTGGTTGGAACGTCAGATCCCTCCAGTAGACCCTCCTGTGCCAGCAACCAATGCATTGAAAACTTTTCAGTTTACTCCTCCAAGACTAGGATCAGAGCGAAAGCCAAGCCCCCTGGCCCAGTCAAGCAATCAAGGGGAACATCCTTTTCCATTTGATAACAATTTTGACACACCAAAATCGTCAGCAACAACCATTAATCTGACAACAATAAAACCAGCACGAACCCCGCCTCCTACTAATATGAGTAGGACTCCACAAGCAAACAGGTCTGGGCTATCGAAGTACAGAAGAACACCAGCTGATGGAGCTGAATCCCCTTTCGGTTTTCCACTCAAGGATGATGCCAGCCTCACAAGCTGCCCCCCATTTTCGGTTCCAAACTACATGGCTCCCACGGCATCAGCCAAAGCGAAAGCACGAGCTAGTAGCAATCCCAGGGATAGATTTGTGGGGACACCGACCAGTGAGTCCAAAAGGAGGCTTTCCTTCCCTTTGACACAAGGTATTGGGTCTTTCAAGTGGAACAAAGCTTCTTTTTTCTCTAACAACAAGGATTCCAGCTCTCCAATGAATTTAGATAAGAACCGGTCTTTGCAATCTATCGGAAACACGAGCATAGATTCCACGGTTTCGATGCCTGCGGCTCCACGAGCTGGGAGGAAGCCGTTTAAACGATTTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4005 IQ

Protein Analysis

576

Amino Acids

64.39

Weight (kDa)

10.94

Isoelectric Point (pI)

65.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IQ	PF00612	184 - 204	2.4e-06	IQ calmodulin-binding motif
DUF4005	PF13178	433 - 535	5.3e-12	Protein of unknown function (DUF4005)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006182)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G59690 AT3G59690
fragaria_vesca	FvH4_4g10360 FvH4_4g10360 FvH4_4g10360 FvH4_4g10360 FvH4_4g10360 FvH4_4g10360
malus_domestica	MD04G1049400.v1.1 MD13G1257200.v1.1
prunus_persica	Prupe.1G207600_v2.0.a1 Prupe.1G207600_v2.0.a1
pyrus_communis	pycom04g04360 pycom13g26240
rosa_chinensis	RchiOBHm_Chr4g0405911
rosa_laevigata	RLG00000008817
rosa_multiflora	Rmu_sc0001200.1_g000002
rosa_roxburghii	Rroxscaffold_5G00350570
rosa_rugosa	Rorug04G0063300 Rorug04G0063400.1 Rorug04G0063500 Rorug04G0063600
rosa_samantha	Rh4AG134200 Rh4BG129500 Rh4CG142100 Rh4DG128700
rosa_wichuraiana	Rw4G010940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	80, 1510
AccB7I	CCANNNNNTGG	2 cut(s)	1346, 1697
AccI	GTMKAC	1 cut(s)	1071
AciI	CCGC	4 cut(s)	27, 167, 1271, 1685
AclWI	GGATC	2 cut(s)	1054, 1141
AcoI	YGGCCR	1 cut(s)	843
AcsI	RAATTY	4 cut(s)	198, 567, 723, 1615
AfaI	GTAC	1 cut(s)	1328
AgeI	ACCGGT	1 cut(s)	1629
AgsI	TTSAA	3 cut(s)	638, 1102, 1561
AhdI	GACNNNNNGTC	1 cut(s)	770
AjnI	CCWGG	2 cut(s)	1155, 1478
AloI	GAACNNNNNNTCC	4 cut(s)	1044, 1076, 1258, 1290
Alw21I	GWGCWC	8 cut(s)	358, 388, 418, 433, 451, 466, 481, 616
Alw26I	GTCTC	3 cut(s)	143, 723, 833
AlwI	GGATC	2 cut(s)	1054, 1141
AoxI	GGCC	4 cut(s)	242, 652, 843, 1158
ApeKI	GCWGC	3 cut(s)	186, 326, 1401
ApoI	RAATTY	4 cut(s)	198, 567, 723, 1615
AseI	ATTAAT	1 cut(s)	1239
AsiGI	ACCGGT	1 cut(s)	1629
AspS9I	GGNCC	1 cut(s)	1159
AsuHPI	GGTGA	1 cut(s)	309
BauI	CACGAG	3 cut(s)	1461, 1654, 1692
BbsI	GAAGAC	1 cut(s)	333
Bbv12I	GWGCWC	8 cut(s)	358, 388, 418, 433, 451, 466, 481, 616
BbvCI	CCTCAGC	1 cut(s)	556
BbvI	GCAGC	3 cut(s)	198, 313, 1388
BccI	CCATC	1 cut(s)	1340
BceAI	ACGGC	3 cut(s)	858, 1455, 1693
BciT130I	CCWGG	2 cut(s)	1157, 1480
BcoDI	GTCTC	3 cut(s)	143, 723, 833
BfaI	CTAG	5 cut(s)	291, 489, 626, 1130, 1467
BfmI	CTRYAG	3 cut(s)	520, 580, 1002
BisI	GCNGC	4 cut(s)	187, 327, 1402, 1686
BlsI	GCNGC	4 cut(s)	188, 328, 1403, 1687
Bme1390I	CCNGG	2 cut(s)	1157, 1480
BmeRI	GACNNNNNGTC	1 cut(s)	770
BmgT120I	GGNCC	1 cut(s)	1159
BmiI	GGNNCC	4 cut(s)	545, 1419, 1434, 1689
BmrFI	CCNGG	2 cut(s)	1157, 1480
BmrI	ACTGGG	1 cut(s)	1156
BmsI	GCATC	3 cut(s)	1375, 1451, 1668
BmuI	ACTGGG	1 cut(s)	1156
BpiI	GAAGAC	1 cut(s)	333
BplI	GAGNNNNNCTC	2 cut(s)	1279, 1311
BpmI	CTGGAG	1 cut(s)	1050
Bpu10I	CCTNAGC	1 cut(s)	556
BpuEI	CTTGAG	2 cut(s)	844, 1361
BsaI	GGTCTC	1 cut(s)	143
BsaWI	WCCGGW	2 cut(s)	1014, 1629
BsaXI	ACNNNNNCTCC	6 cut(s)	884, 914, 941, 971, 1258, 1288
Bse118I	RCCGGY	1 cut(s)	1629
Bse1I	ACTGG	4 cut(s)	1049, 1067, 1162, 1506
Bse3DI	GCAATG	1 cut(s)	948
BseBI	CCWGG	2 cut(s)	1157, 1480
BseGI	GGATG	3 cut(s)	868, 1183, 1387
BseMI	GCAATG	1 cut(s)	948
BseMII	CTCAG	2 cut(s)	570, 828
BseNI	ACTGG	4 cut(s)	1049, 1067, 1162, 1506
BseRI	GAGGAG	7 cut(s)	39, 429, 441, 444, 456, 459, 1110
BseXI	GCAGC	3 cut(s)	198, 313, 1388
BseYI	CCCAGC	1 cut(s)	1697
BshFI	GGCC	4 cut(s)	244, 654, 845, 1160
BshTI	ACCGGT	1 cut(s)	1629
BsiHKAI	GWGCWC	8 cut(s)	358, 388, 418, 433, 451, 466, 481, 616
BsiSI	CCGG	2 cut(s)	1015, 1630
BslFI	GGGAC	1 cut(s)	1510
BsmAI	GTCTC	3 cut(s)	143, 723, 833
BsmBI	CGTCTC	1 cut(s)	833
BsmFI	GGGAC	1 cut(s)	1510
BsmI	GAATGC	1 cut(s)	685
BsnI	GGCC	4 cut(s)	244, 654, 845, 1160
Bso31I	GGTCTC	1 cut(s)	143
Bsp143I	GATC	2 cut(s)	1059, 1133
BspACI	CCGC	4 cut(s)	27, 167, 1271, 1685
BspANI	GGCC	4 cut(s)	244, 654, 845, 1160
BspCNI	CTCAG	2 cut(s)	569, 829
BspHI	TCATGA	1 cut(s)	924
BspLI	GGNNCC	4 cut(s)	545, 1419, 1434, 1689
BspPI	GGATC	2 cut(s)	1054, 1141
BspTNI	GGTCTC	1 cut(s)	143
BsrDI	GCAATG	1 cut(s)	948
BsrFI	RCCGGY	1 cut(s)	1629
BsrI	ACTGG	4 cut(s)	1049, 1067, 1162, 1506
BssAI	RCCGGY	1 cut(s)	1629
BssMI	GATC	2 cut(s)	1059, 1133
BssSI	CACGAG	3 cut(s)	1461, 1654, 1692
BssT1I	CCWWGG	5 cut(s)	57, 320, 335, 365, 395
Bst2BI	CACGAG	3 cut(s)	1461, 1654, 1692
Bst2UI	CCWGG	2 cut(s)	1157, 1480
Bst4CI	ACNGT	1 cut(s)	1672
BstAPI	GCANNNNNTGC	1 cut(s)	1094
BstC8I	GCNNGC	5 cut(s)	830, 872, 1086, 1390, 1683
BstDEI	CTNAG	4 cut(s)	539, 556, 837, 879
BstDSI	CCRYGG	3 cut(s)	1031, 1437, 1668
BstENI	CCTNNNNNAGG	1 cut(s)	987
BstF5I	GGATG	3 cut(s)	868, 1183, 1387
BstKTI	GATC	2 cut(s)	1062, 1136
BstMAI	GTCTC	3 cut(s)	143, 723, 833
BstMBI	GATC	2 cut(s)	1059, 1133
BstMWI	GCNNNNNNNGC	6 cut(s)	810, 1094, 1349, 1398, 1439, 1694
BstNI	CCWGG	2 cut(s)	1157, 1480
BstNSI	RCATGY	1 cut(s)	697
BstSCI	CCNGG	2 cut(s)	1155, 1478
BstSFI	CTRYAG	3 cut(s)	520, 580, 1002
BstV1I	GCAGC	3 cut(s)	198, 313, 1388
BstV2I	GAAGAC	1 cut(s)	333
BstX2I	RGATCY	1 cut(s)	1059
BstYI	RGATCY	1 cut(s)	1059
BsuRI	GGCC	4 cut(s)	244, 654, 845, 1160
BtgI	CCRYGG	3 cut(s)	1031, 1437, 1668
BtsCI	GGATG	3 cut(s)	868, 1183, 1387
BtsIMutI	CAGTG	1 cut(s)	1513
Cac8I	GCNNGC	5 cut(s)	830, 872, 1086, 1390, 1683
CciI	TCATGA	1 cut(s)	924
Cfr10I	RCCGGY	1 cut(s)	1629
Cfr13I	GGNCC	1 cut(s)	1159
CseI	GACGC	1 cut(s)	656
Csp6I	GTAC	1 cut(s)	1327
CspAI	ACCGGT	1 cut(s)	1629
CviAII	CATG	5 cut(s)	551, 685, 694, 925, 1429
CviQI	GTAC	1 cut(s)	1327
DdeI	CTNAG	4 cut(s)	539, 556, 837, 879
DpnI	GATC	2 cut(s)	1061, 1135
DpnII	GATC	2 cut(s)	1059, 1133
DraI	TTTAAA	1 cut(s)	1714
DrdI	GACNNNNNNGTC	2 cut(s)	80, 1510
DriI	GACNNNNNGTC	1 cut(s)	770
DseDI	GACNNNNNNGTC	2 cut(s)	80, 1510
EaeI	YGGCCR	1 cut(s)	843
Eam1105I	GACNNNNNGTC	1 cut(s)	770
EciI	GGCGGA	1 cut(s)	16
Ecl136II	GAGCTC	8 cut(s)	356, 386, 416, 431, 449, 464, 479, 614
Eco130I	CCWWGG	5 cut(s)	57, 320, 335, 365, 395
Eco147I	AGGCCT	1 cut(s)	244
Eco31I	GGTCTC	1 cut(s)	143
Eco32I	GATATC	1 cut(s)	1012
Eco53kI	GAGCTC	8 cut(s)	356, 386, 416, 431, 449, 464, 479, 614
EcoICRI	GAGCTC	8 cut(s)	356, 386, 416, 431, 449, 464, 479, 614
EcoNI	CCTNNNNNAGG	1 cut(s)	987
EcoRII	CCWGG	2 cut(s)	1155, 1478
EcoRV	GATATC	1 cut(s)	1012
EcoT14I	CCWWGG	5 cut(s)	57, 320, 335, 365, 395
EcoT22I	ATGCAT	1 cut(s)	1099
ErhI	CCWWGG	5 cut(s)	57, 320, 335, 365, 395
Esp3I	CGTCTC	1 cut(s)	833
FaeI	CATG	5 cut(s)	554, 688, 697, 928, 1432
FaqI	GGGAC	1 cut(s)	1510
FatI	CATG	5 cut(s)	550, 684, 693, 924, 1428
FauI	CCCGC	1 cut(s)	1278
FblI	GTMKAC	1 cut(s)	1071
Fnu4HI	GCNGC	4 cut(s)	187, 327, 1402, 1686
FokI	GGATG	3 cut(s)	875, 1170, 1394
Fsp4HI	GCNGC	4 cut(s)	187, 327, 1402, 1686
FspBI	CTAG	5 cut(s)	291, 489, 626, 1130, 1467
GluI	GCNGC	4 cut(s)	187, 327, 1402, 1686
GsaI	CCCAGC	1 cut(s)	1701
GsuI	CTGGAG	1 cut(s)	1050
HaeIII	GGCC	4 cut(s)	244, 654, 845, 1160
HapII	CCGG	2 cut(s)	1015, 1630
HgaI	GACGC	1 cut(s)	656
Hin1II	CATG	5 cut(s)	554, 688, 697, 928, 1432
HindIII	AAGCTT	2 cut(s)	604, 1572
HinfI	GANTC	6 cut(s)	739, 1294, 1355, 1511, 1598, 1664
HpaII	CCGG	2 cut(s)	1015, 1630
HphI	GGTGA	1 cut(s)	309
Hpy166II	GTNNAC	3 cut(s)	317, 1072, 1116
Hpy188III	TCNNGA	1 cut(s)	925
Hpy8I	GTNNAC	3 cut(s)	317, 1072, 1116
HpyAV	CCTTC	6 cut(s)	115, 369, 399, 817, 967, 1540
HpyCH4III	ACNGT	1 cut(s)	1672
HpyCH4IV	ACGT	2 cut(s)	660, 1054
HpyCH4V	TGCA	4 cut(s)	697, 904, 1097, 1639
HpyF10VI	GCNNNNNNNGC	6 cut(s)	810, 1094, 1349, 1398, 1439, 1694
HpyF3I	CTNAG	4 cut(s)	539, 556, 837, 879
HpySE526I	ACGT	2 cut(s)	660, 1054
Hsp92II	CATG	5 cut(s)	554, 688, 697, 928, 1432
Kzo9I	GATC	2 cut(s)	1059, 1133
Lsp1109I	GCAGC	3 cut(s)	198, 313, 1388
LweI	GCATC	3 cut(s)	1375, 1451, 1668
MaeI	CTAG	5 cut(s)	291, 489, 626, 1130, 1467
MaeII	ACGT	2 cut(s)	660, 1054
MaeIII	GTNAC	2 cut(s)	232, 729
MalI	GATC	2 cut(s)	1061, 1135
MboI	GATC	2 cut(s)	1059, 1133
MboII	GAAGA	5 cut(s)	130, 333, 336, 612, 1344
MflI	RGATCY	1 cut(s)	1059
MluCI	AATT	6 cut(s)	154, 198, 567, 723, 1204, 1615
MlyI	GAGTC	2 cut(s)	1288, 1520
MmeI	TCCRAC	2 cut(s)	984, 1029
Mph1103I	ATGCAT	1 cut(s)	1099
MseI	TTAA	2 cut(s)	1239, 1713
MspA1I	CMGCKG	3 cut(s)	169, 967, 1343
MspI	CCGG	2 cut(s)	1015, 1630
MspR9I	CCNGG	2 cut(s)	1157, 1480
MssI	GTTTAAAC	1 cut(s)	1714
Mva1269I	GAATGC	1 cut(s)	685
MvaI	CCWGG	2 cut(s)	1157, 1480
MwoI	GCNNNNNNNGC	6 cut(s)	810, 1094, 1349, 1398, 1439, 1694
NdeII	GATC	2 cut(s)	1059, 1133
NlaIII	CATG	5 cut(s)	554, 688, 697, 928, 1432
NlaIV	GGNNCC	4 cut(s)	545, 1419, 1434, 1689
NmuCI	GTSAC	1 cut(s)	729
NsiI	ATGCAT	1 cut(s)	1099
NspI	RCATGY	1 cut(s)	697
PagI	TCATGA	1 cut(s)	924
PasI	CCCWGGG	1 cut(s)	1479
PceI	AGGCCT	1 cut(s)	244
PctI	GAATGC	1 cut(s)	685
PfeI	GAWTC	4 cut(s)	739, 1355, 1598, 1664
PflMI	CCANNNNNTGG	2 cut(s)	1346, 1697
PinAI	ACCGGT	1 cut(s)	1629
PkrI	GCNGC	4 cut(s)	188, 328, 1403, 1687
PleI	GAGTC	2 cut(s)	1288, 1519
PmeI	GTTTAAAC	1 cut(s)	1714
PpsI	GAGTC	2 cut(s)	1288, 1519
PshBI	ATTAAT	1 cut(s)	1239
Psp124BI	GAGCTC	8 cut(s)	358, 388, 418, 433, 451, 466, 481, 616
Psp6I	CCWGG	2 cut(s)	1155, 1478
PspFI	CCCAGC	1 cut(s)	1697
PspGI	CCWGG	2 cut(s)	1155, 1478
PspN4I	GGNNCC	4 cut(s)	545, 1419, 1434, 1689
PspPI	GGNCC	1 cut(s)	1159
PsuI	RGATCY	1 cut(s)	1059
PvuII	CAGCTG	2 cut(s)	967, 1343
RsaI	GTAC	1 cut(s)	1328
RsaNI	GTAC	1 cut(s)	1327
SacI	GAGCTC	8 cut(s)	358, 388, 418, 433, 451, 466, 481, 616
SaqAI	TTAA	2 cut(s)	1239, 1713
SatI	GCNGC	4 cut(s)	187, 327, 1402, 1686
Sau3AI	GATC	2 cut(s)	1059, 1133
Sau96I	GGNCC	1 cut(s)	1159
SchI	GAGTC	2 cut(s)	1288, 1520
ScrFI	CCNGG	2 cut(s)	1157, 1480
SfaNI	GCATC	3 cut(s)	1375, 1451, 1668
SfcI	CTRYAG	3 cut(s)	520, 580, 1002
SmlI	CTYRAG	2 cut(s)	823, 1376
SmoI	CTYRAG	2 cut(s)	823, 1376
Sse9I	AATT	6 cut(s)	154, 198, 567, 723, 1204, 1615
SseBI	AGGCCT	1 cut(s)	244
SsiI	CCGC	4 cut(s)	27, 167, 1271, 1685
SspMI	CTAG	5 cut(s)	291, 489, 626, 1130, 1467
SstI	GAGCTC	8 cut(s)	358, 388, 418, 433, 451, 466, 481, 616
StuI	AGGCCT	1 cut(s)	244
StyD4I	CCNGG	2 cut(s)	1155, 1478
StyI	CCWWGG	5 cut(s)	57, 320, 335, 365, 395
TaaI	ACNGT	1 cut(s)	1672
TaiI	ACGT	2 cut(s)	663, 1057
TaqI	TCGA	2 cut(s)	1322, 1676
TasI	AATT	6 cut(s)	154, 198, 567, 723, 1204, 1615
TatI	WGTACW	1 cut(s)	1326
TauI	GCSGC	1 cut(s)	1688
TfiI	GAWTC	4 cut(s)	739, 1355, 1598, 1664
Tru1I	TTAA	2 cut(s)	1239, 1713
Tru9I	TTAA	2 cut(s)	1239, 1713
TscAI	CASTG	1 cut(s)	1513
TseFI	GTSAC	1 cut(s)	729
TseI	GCWGC	3 cut(s)	186, 326, 1401
Tsp45I	GTSAC	1 cut(s)	729
TspDTI	ATGAA	7 cut(s)	147, 151, 488, 692, 701, 941, 1628
TspGWI	ACGGA	2 cut(s)	748, 1020
TspRI	CASTG	1 cut(s)	1513
Van91I	CCANNNNNTGG	2 cut(s)	1346, 1697
VspI	ATTAAT	1 cut(s)	1239
XagI	CCTNNNNNAGG	1 cut(s)	987
XapI	RAATTY	4 cut(s)	198, 567, 723, 1615
XceI	RCATGY	1 cut(s)	697
XmiI	GTMKAC	1 cut(s)	1071
XspI	CTAG	5 cut(s)	291, 489, 626, 1130, 1467
Zsp2I	ATGCAT	1 cut(s)	1099

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.