MD04G1062400.v1.1

Oxidoreductase family, C-terminal alpha/beta domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014074
Physical Location & Seq
Reverse (-)
8206702 .. 8211760
5059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1062400.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGTCAGTGATGGCAGCTTCGAATACAGTCAAGTATGGGATTGTAGGGGTGGGCATGATGGGCAGAGAGCACCTCATCAATTTGTACCACCTTCGCTCCGAAGGCGTGGCCGTGGTCGCCATAGCCGACCCCCATCTTCCTTCCCAGAAGCTTGCCCTTGACTTGGCACACTCTTTTAATTGGCCCATCAAGGTTTTTTCAGGACACAGGGAGCTATTGGACAGCTTGCTCTGTGATGTGGTGGTTGTGTCAACTCCAAATATGACCCATTATCAAATTTTGATGGATATCATCAACCACCGTAAACCCCATCATGTATTAGTGGAGAAGCCATTGTGTACCACGGTTGCTCACTGCAAGGAGGTCGTGAATTCTGCTAGAAAGAGGCAGGATATGCTGGTACAAGTTGGACTGGAGTATAGATACATGCCACCTGTTGCTAAACTGATAGAAATAGTAAAGGGTGGAAGTCTTGGACAGGTCAAAATGGTGGCAATCCGCGAACACCGGTTTCCTTTCTTGGTTAAGGTCAACAATTGGAACCGGTTCAACATTAACTCTGGGGGGACTTTGGTAGAGAAGTGCTGCCACTTCTTTGATCTGATGAGGCTGTTTGCTGGTGCCAACCCAGTTCGTGTCATGGCTTCTGGAGCCATTGACGTTAACCACAAAGATGAAATATACGATGGAAAGGTCCCAGACATAATTGACAATGCATATGTTATCGTTGAATTTGACAATGGTTCTCGCGGGATGCTTGACCTTTGTATGTTTGCTGAAGGAACTAAAAATGAGCAAGAAATATCTGTTGTTGGTGAGATAGGAAAGGGGGAGGCCTTTGTTCCTGAGAGCATTGTACGTTTTGGTACTCGAGTCGATGGGAGAAATGGTGTCCAGACTTTAAAAGCTGAGAATGATCAAATAAAATATGATGGACTGCATCATGGATCTAGCTATTTGGAACACCTGCACTTCTTATCTGCAATTAGGGCCAAAGCTAAAGCTCCAGCAGTAGATTTGCACGACGGATTAGTTTCAGTTGCCATCGGAGTTGCAGCACAGCTTTCAATAGAGAAGGGCCGATTTGTGACGATTGAAGAAGTCATGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA GFO_IDH_MocA_C

Protein Analysis

371

Amino Acids

40.98

Weight (kDa)

6.81

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 11 - 137 1.9e-27 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 147 - 275 1.6e-15 GFO/IDH/MocA C-terminal domain
GFO_IDH_MocA_C PF02894 151 - 364 9.2e-38 Oxidoreductase family, C-terminal alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17370
fragaria_vesca FvH4_5g19170 FvH4_5g19170
malus_domestica MD04G1062400.v1.1
prunus_persica Prupe.5G066000_v2.0.a1 Prupe.5G066000_v2.0.a1 Prupe.5G066000_v2.0.a1
pyrus_communis pycom04g05590
rosa_chinensis RchiOBHm_Chr7g0203711
rosa_laevigata RLG00000003511
rosa_multiflora Rmu_sc0001714.1_g000005 Rmu_sc0005354.1_g000002
rosa_roxburghii Rroxscaffold_3G00253600
rosa_rugosa Rorug07G0078100.1
rosa_samantha Rh7BG207000 Rh7DG214900
rosa_wichuraiana Rw7G018120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 975
Acc36I ACCTGC 1 cut(s) 975
AccB1I GGYRCC 1 cut(s) 620
AccII CGCG 2 cut(s) 501, 750
AciI CCGC 2 cut(s) 499, 750
AclWI GGATC 1 cut(s) 955
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 3 cut(s) 276, 370, 731
AcuI CTGAAG 1 cut(s) 798
AfaI GTAC 5 cut(s) 86, 340, 402, 858, 868
AfiI CCNNNNNNNGG 1 cut(s) 163
AgeI ACCGGT 2 cut(s) 507, 543
AgsI TTSAA 4 cut(s) 550, 731, 1068, 1097
AluBI AGCT 9 cut(s) 17, 151, 214, 225, 908, 954, 998, 1004, 1063
AluI AGCT 9 cut(s) 17, 151, 214, 225, 908, 954, 998, 1004, 1063
Alw21I GWGCWC 1 cut(s) 72
AlwI GGATC 1 cut(s) 955
Ama87I CYCGRG 1 cut(s) 870
AoxI GGCC 5 cut(s) 108, 182, 834, 990, 1078
ApeKI GCWGC 3 cut(s) 14, 585, 1055
ApoI RAATTY 3 cut(s) 276, 370, 731
AsiGI ACCGGT 2 cut(s) 507, 543
Asp700I GAANNNNTTC 1 cut(s) 545
AspS9I GGNCC 4 cut(s) 183, 694, 990, 1078
AsuHPI GGTGA 1 cut(s) 827
AsuII TTCGAA 1 cut(s) 20
AvaI CYCGRG 1 cut(s) 870
AvaII GGWCC 1 cut(s) 694
BaeI ACNNNNGTAYC 2 cut(s) 858, 891
BanI GGYRCC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 3 cut(s) 26, 572, 1067
BceAI ACGGC 1 cut(s) 95
BcgI CGANNNNNNTGC 2 cut(s) 346, 380
BclI TGATCA 1 cut(s) 916
BfaI CTAG 2 cut(s) 378, 951
BfuAI ACCTGC 1 cut(s) 975
BisI GCNGC 3 cut(s) 15, 586, 1056
BlsI GCNGC 3 cut(s) 16, 587, 1057
Bme18I GGWCC 1 cut(s) 694
BmeT110I CYCGRG 1 cut(s) 870
BmgT120I GGNCC 4 cut(s) 183, 694, 990, 1078
BmiI GGNNCC 4 cut(s) 542, 622, 652, 696
BmrI ACTGGG 1 cut(s) 623
BmsI GCATC 2 cut(s) 744, 949
BmuI ACTGGG 1 cut(s) 623
BplI GAGNNNNNCTC 2 cut(s) 57, 89
BpmI CTGGAG 3 cut(s) 434, 669, 990
Bpu14I TTCGAA 1 cut(s) 20
BsaBI GATNNNNATC 1 cut(s) 287
BsaJI CCNNGG 2 cut(s) 111, 342
BsaWI WCCGGW 2 cut(s) 507, 543
BsaXI ACNNNNNCTCC 4 cut(s) 80, 110, 874, 904
Bsc4I CCNNNNNNNGG 1 cut(s) 163
Bse118I RCCGGY 2 cut(s) 507, 543
Bse1I ACTGG 2 cut(s) 417, 629
Bse8I GATNNNNATC 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 111, 342
BseGI GGATG 1 cut(s) 759
BseJI GATNNNNATC 1 cut(s) 287
BseLI CCNNNNNNNGG 1 cut(s) 163
BseMII CTCAG 2 cut(s) 837, 900
BseNI ACTGG 2 cut(s) 417, 629
BseXI GCAGC 3 cut(s) 26, 572, 1067
BsgI GTGCAG 1 cut(s) 953
Bsh1236I CGCG 2 cut(s) 501, 750
BshFI GGCC 5 cut(s) 110, 184, 836, 992, 1080
BshNI GGYRCC 1 cut(s) 620
BshTI ACCGGT 2 cut(s) 507, 543
BsiHKAI GWGCWC 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 870
BsiSI CCGG 2 cut(s) 508, 544
BslFI GGGAC 2 cut(s) 580, 680
BslI CCNNNNNNNGG 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 580, 680
BsnI GGCC 5 cut(s) 110, 184, 836, 992, 1080
BsoBI CYCGRG 1 cut(s) 870
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 3 cut(s) 598, 916, 947
BspACI CCGC 2 cut(s) 499, 750
BspANI GGCC 5 cut(s) 110, 184, 836, 992, 1080
BspCNI CTCAG 2 cut(s) 838, 901
BspFNI CGCG 2 cut(s) 501, 750
BspHI TCATGA 1 cut(s) 1104
BspLI GGNNCC 4 cut(s) 542, 622, 652, 696
BspMI ACCTGC 1 cut(s) 975
BspPI GGATC 1 cut(s) 955
BspT104I TTCGAA 1 cut(s) 20
BspT107I GGYRCC 1 cut(s) 620
BsrFI RCCGGY 2 cut(s) 507, 543
BsrI ACTGG 2 cut(s) 417, 629
BssAI RCCGGY 2 cut(s) 507, 543
BssECI CCNNGG 2 cut(s) 111, 342
BssMI GATC 3 cut(s) 598, 916, 947
Bst4CI ACNGT 3 cut(s) 28, 302, 346
BstAPI GCANNNNNTGC 1 cut(s) 394
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 153, 227
BstDEI CTNAG 2 cut(s) 846, 909
BstDSI CCRYGG 2 cut(s) 111, 342
BstF5I GGATG 1 cut(s) 759
BstFNI CGCG 2 cut(s) 501, 750
BstKTI GATC 3 cut(s) 601, 919, 950
BstMBI GATC 3 cut(s) 598, 916, 947
BstMWI GCNNNNNNNGC 6 cut(s) 60, 102, 116, 394, 650, 989
BstNSI RCATGY 1 cut(s) 430
BstUI CGCG 2 cut(s) 501, 750
BstV1I GCAGC 3 cut(s) 26, 572, 1067
BstX2I RGATCY 1 cut(s) 947
BstYI RGATCY 1 cut(s) 947
BsuRI GGCC 5 cut(s) 110, 184, 836, 992, 1080
BtgI CCRYGG 2 cut(s) 111, 342
BtsCI GGATG 1 cut(s) 759
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 2 cut(s) 12, 352
BveI ACCTGC 1 cut(s) 975
Cac8I GCNNGC 2 cut(s) 153, 227
CciI TCATGA 1 cut(s) 1104
Cfr10I RCCGGY 2 cut(s) 507, 543
Cfr13I GGNCC 4 cut(s) 183, 694, 990, 1078
Csp6I GTAC 5 cut(s) 85, 339, 401, 857, 867
CspAI ACCGGT 2 cut(s) 507, 543
CviAII CATG 6 cut(s) 55, 314, 427, 640, 944, 1105
CviQI GTAC 5 cut(s) 85, 339, 401, 857, 867
DdeI CTNAG 2 cut(s) 846, 909
DpnI GATC 3 cut(s) 600, 918, 949
DpnII GATC 3 cut(s) 598, 916, 947
DraI TTTAAA 1 cut(s) 903
EaeI YGGCCR 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 836
Eco32I GATATC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 694
Eco57I CTGAAG 1 cut(s) 798
Eco88I CYCGRG 1 cut(s) 870
EcoO109I RGGNCCY 1 cut(s) 694
EcoRI GAATTC 1 cut(s) 370
EcoRV GATATC 1 cut(s) 289
EcoT22I ATGCAT 1 cut(s) 718
FaeI CATG 6 cut(s) 58, 317, 430, 643, 947, 1108
FaqI GGGAC 2 cut(s) 580, 680
FatI CATG 6 cut(s) 54, 313, 426, 639, 943, 1104
FauI CCCGC 1 cut(s) 743
FauNDI CATATG 1 cut(s) 718
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 3 cut(s) 15, 586, 1056
FokI GGATG 1 cut(s) 766
Fsp4HI GCNGC 3 cut(s) 15, 586, 1056
FspBI CTAG 2 cut(s) 378, 951
GluI GCNGC 3 cut(s) 15, 586, 1056
GsuI CTGGAG 3 cut(s) 434, 669, 990
HaeIII GGCC 5 cut(s) 110, 184, 836, 992, 1080
HapII CCGG 2 cut(s) 508, 544
Hin1II CATG 6 cut(s) 58, 317, 430, 643, 947, 1108
HincII GTYRAC 3 cut(s) 252, 532, 664
HindII GTYRAC 3 cut(s) 252, 532, 664
HindIII AAGCTT 1 cut(s) 149
HinfI GANTC 1 cut(s) 873
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 2 cut(s) 508, 544
HphI GGTGA 1 cut(s) 827
Hpy166II GTNNAC 5 cut(s) 252, 305, 339, 532, 664
Hpy188I TCNGA 3 cut(s) 100, 603, 1049
Hpy188III TCNNGA 6 cut(s) 201, 367, 648, 845, 895, 1105
Hpy8I GTNNAC 5 cut(s) 252, 305, 339, 532, 664
Hpy99I CGWCG 1 cut(s) 1028
HpyAV CCTTC 5 cut(s) 95, 101, 150, 773, 1069
HpyCH4III ACNGT 3 cut(s) 28, 302, 346
HpyCH4IV ACGT 2 cut(s) 660, 859
HpyCH4V TGCA 7 cut(s) 357, 716, 940, 970, 983, 1021, 1055
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 102, 116, 394, 650, 989
HpyF3I CTNAG 2 cut(s) 846, 909
HpySE526I ACGT 2 cut(s) 660, 859
Hsp92II CATG 6 cut(s) 58, 317, 430, 643, 947, 1108
Ksp22I TGATCA 1 cut(s) 916
KspAI GTTAAC 1 cut(s) 664
Kzo9I GATC 3 cut(s) 598, 916, 947
LmnI GCTCC 4 cut(s) 101, 211, 650, 1009
Lsp1109I GCAGC 3 cut(s) 26, 572, 1067
LweI GCATC 2 cut(s) 744, 949
MaeI CTAG 2 cut(s) 378, 951
MaeII ACGT 2 cut(s) 660, 859
MaeIII GTNAC 1 cut(s) 1087
MalI GATC 3 cut(s) 600, 918, 949
MboI GATC 3 cut(s) 598, 916, 947
MboII GAAGA 2 cut(s) 128, 1109
MfeI CAATTG 1 cut(s) 535
MflI RGATCY 1 cut(s) 947
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 9 cut(s) 79, 178, 276, 370, 535, 705, 731, 984, 1108
MlyI GAGTC 1 cut(s) 882
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 5 cut(s) 83, 355, 378, 600, 826
Mph1103I ATGCAT 1 cut(s) 718
MroXI GAANNNNTTC 1 cut(s) 545
MseI TTAA 6 cut(s) 177, 525, 555, 663, 902, 1111
MslI CAYNNNNRTG 1 cut(s) 672
MspI CCGG 2 cut(s) 508, 544
MunI CAATTG 1 cut(s) 535
MvnI CGCG 2 cut(s) 501, 750
MwoI GCNNNNNNNGC 6 cut(s) 60, 102, 116, 394, 650, 989
NdeI CATATG 1 cut(s) 718
NdeII GATC 3 cut(s) 598, 916, 947
NlaIII CATG 6 cut(s) 58, 317, 430, 643, 947, 1108
NlaIV GGNNCC 4 cut(s) 542, 622, 652, 696
NmuCI GTSAC 1 cut(s) 1087
NsiI ATGCAT 1 cut(s) 718
NspI RCATGY 1 cut(s) 430
NspV TTCGAA 1 cut(s) 20
PaeR7I CTCGAG 1 cut(s) 870
PagI TCATGA 1 cut(s) 1104
PaqCI CACCTGC 1 cut(s) 975
PceI AGGCCT 1 cut(s) 836
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 545
PinAI ACCGGT 2 cut(s) 507, 543
PkrI GCNGC 3 cut(s) 16, 587, 1057
PleI GAGTC 1 cut(s) 881
PpsI GAGTC 1 cut(s) 881
PpuMI RGGWCCY 1 cut(s) 694
Psp5II RGGWCCY 1 cut(s) 694
PspN4I GGNNCC 4 cut(s) 542, 622, 652, 696
PspPI GGNCC 4 cut(s) 183, 694, 990, 1078
PspPPI RGGWCCY 1 cut(s) 694
PspXI VCTCGAGB 1 cut(s) 870
PsuI RGATCY 1 cut(s) 947
RsaI GTAC 5 cut(s) 86, 340, 402, 858, 868
RsaNI GTAC 5 cut(s) 85, 339, 401, 857, 867
RseI CAYNNNNRTG 1 cut(s) 672
SaqAI TTAA 6 cut(s) 177, 525, 555, 663, 902, 1111
SatI GCNGC 3 cut(s) 15, 586, 1056
Sau3AI GATC 3 cut(s) 598, 916, 947
Sau96I GGNCC 4 cut(s) 183, 694, 990, 1078
SchI GAGTC 1 cut(s) 882
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 2 cut(s) 744, 949
Sfr274I CTCGAG 1 cut(s) 870
SfuI TTCGAA 1 cut(s) 20
SinI GGWCC 1 cut(s) 694
SlaI CTCGAG 1 cut(s) 870
SmiMI CAYNNNNRTG 1 cut(s) 672
SmlI CTYRAG 1 cut(s) 870
SmoI CTYRAG 1 cut(s) 870
Sse9I AATT 9 cut(s) 79, 178, 276, 370, 535, 705, 731, 984, 1108
SseBI AGGCCT 1 cut(s) 836
SsiI CCGC 2 cut(s) 499, 750
SspMI CTAG 2 cut(s) 378, 951
StuI AGGCCT 1 cut(s) 836
TaaI ACNGT 3 cut(s) 28, 302, 346
TaiI ACGT 2 cut(s) 663, 862
TaqI TCGA 3 cut(s) 20, 871, 876
TasI AATT 9 cut(s) 79, 178, 276, 370, 535, 705, 731, 984, 1108
Tru1I TTAA 6 cut(s) 177, 525, 555, 663, 902, 1111
Tru9I TTAA 6 cut(s) 177, 525, 555, 663, 902, 1111
TscAI CASTG 2 cut(s) 12, 359
TseFI GTSAC 1 cut(s) 1087
TseI GCWGC 3 cut(s) 14, 585, 1055
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 1 cut(s) 690
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 2 cut(s) 12, 359
VpaK11BI GGWCC 1 cut(s) 694
XapI RAATTY 3 cut(s) 276, 370, 731
XceI RCATGY 1 cut(s) 430
XhoI CTCGAG 1 cut(s) 870
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 2 cut(s) 378, 951
Zsp2I ATGCAT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.