Prupe.5G066000_v2.0.a1

Oxidoreductase family, C-terminal alpha/beta domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0014074
Physical Location & Seq
Forward (+)
8064410 .. 8068009
3600 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G066000.1

Sequence Viewer

Length: 1104 bp
ATGGCTGCTTCAAATATAGTCAAGTATGGGATTGTAGGGGTGGGCATGATGGGCAGAGAGCACCTCATCAACTTGCACCATCTTCGCACCGAAGGCGTGGCGGTGGTAGCCATAGCTGACCCCCATGTTCCTTCCCAAAAGCTTGCTCTTGATTTGGCACAGTCTTTTAATTGGCCTATAAAGGTTTTTTCAGCACACAAGGAACTATTGGACAGCCTGCTCTGTGATGTAGTGGTTGTGTCATCTCCAAATATGACCCATTATCAAATTCTGATGGATATCATCAACCACCCCAAACCCCACCATGTACTAGTGGAGAAGCCATTGTGTACAACAGTAGCTCACTGCAAAGAGGTTGTAAATGCTGCCAGAAAGAGGCAGGATATTCTGGTTCAAGTTGGACTGGAGTATAGATACATGCCACCTGTTGCTAAACTGATAGAAATAGTGAAGGGCGGAAGCCTTGGACAGGTCAAAATGGTGGCAATCCGTGAACATCGTTTTCCTTTCTTGGTTAAGGTCAACAATTGGAACCGGTTCAACATTAACTCTGGAGGGACTCTGGTAGAAAAGTGCTGCCACTTCTTTGATCTGATGAGGCTGTTTGCTGGTGCTAACCCTGTTCGTGTGATGGCTTCTGGAGCGATTGATGTTAACCACAAAGATGAAATATATGATGGAAAGGTTCCAGACATAATTGACAATGCATATGTTATTGTCGAATTTGACAATGGTTCTCGCGGGATGCTTGACCTTTGTATGTTTGCTGAAGGGACTAAAAATGAACAAGAAATATCTGTTGTTGGTGACATAGGAAAGGGGGAGGCCTTTGTTCCTGAGAGTATTGTTCGTCATGGCACTCGAGTGGAAGGGAGAGATGGTGTCCAGACTTTAAAAGCTGAGAATGATCAAATAAAATATGATGGGCTGCATCATGGATCAAGCTATTTGGAACACCTGCACTTCTTATCTGCAATAAGGGCTAAAGCAAAAGCGCCTGCTGTAGATTTGCATGATGGATTAGTTTCGGTTGCCATTGGAGTTGCAGCACAGCTTTCCATCGAGAAGGGCCGATTTGTGACGATTGAAGAAGTCATGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA GFO_IDH_MocA_C

Protein Analysis

368

Amino Acids

40.48

Weight (kDa)

6.55

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17370
fragaria_vesca FvH4_5g19170 FvH4_5g19170
malus_domestica MD04G1062400.v1.1
prunus_persica Prupe.5G066000_v2.0.a1 Prupe.5G066000_v2.0.a1 Prupe.5G066000_v2.0.a1
pyrus_communis pycom04g05590
rosa_chinensis RchiOBHm_Chr7g0203711
rosa_laevigata RLG00000003511
rosa_multiflora Rmu_sc0001714.1_g000005 Rmu_sc0005354.1_g000002
rosa_roxburghii Rroxscaffold_3G00253600
rosa_rugosa Rorug07G0078100.1
rosa_samantha Rh7BG207000 Rh7DG214900
rosa_wichuraiana Rw7G018120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 966
Acc36I ACCTGC 1 cut(s) 966
AccII CGCG 1 cut(s) 741
AciI CCGC 3 cut(s) 101, 456, 741
AclWI GGATC 1 cut(s) 946
AcsI RAATTY 2 cut(s) 267, 722
AcuI CTGAAG 1 cut(s) 789
AfaI GTAC 2 cut(s) 309, 331
AfiI CCNNNNNNNGG 2 cut(s) 375, 469
AgeI ACCGGT 1 cut(s) 534
AgsI TTSAA 4 cut(s) 12, 395, 541, 1088
AhlI ACTAGT 1 cut(s) 310
AleI CACNNNNGTG 1 cut(s) 863
AluBI AGCT 6 cut(s) 116, 142, 341, 899, 945, 1054
AluI AGCT 6 cut(s) 116, 142, 341, 899, 945, 1054
Alw21I GWGCWC 1 cut(s) 63
AlwI GGATC 1 cut(s) 946
Ama87I CYCGRG 1 cut(s) 861
AoxI GGCC 3 cut(s) 173, 825, 1069
ApeKI GCWGC 5 cut(s) 5, 365, 576, 928, 1046
ApoI RAATTY 2 cut(s) 267, 722
AsiGI ACCGGT 1 cut(s) 534
Asp700I GAANNNNTTC 1 cut(s) 536
AspLEI GCGC 1 cut(s) 997
AspS9I GGNCC 1 cut(s) 1069
AsuHPI GGTGA 1 cut(s) 818
AvaI CYCGRG 1 cut(s) 861
Bbv12I GWGCWC 1 cut(s) 63
BbvI GCAGC 4 cut(s) 352, 563, 915, 1058
BccI CCATC 9 cut(s) 43, 87, 268, 625, 671, 872, 917, 1010, 1067
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BclI TGATCA 1 cut(s) 907
BcuI ACTAGT 1 cut(s) 310
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 1002
BfoI RGCGCY 1 cut(s) 998
BfuAI ACCTGC 1 cut(s) 966
BisI GCNGC 5 cut(s) 6, 366, 577, 929, 1047
BlsI GCNGC 5 cut(s) 7, 367, 578, 930, 1048
BmeT110I CYCGRG 1 cut(s) 861
BmgT120I GGNCC 1 cut(s) 1069
BmiI GGNNCC 2 cut(s) 533, 687
BmsI GCATC 2 cut(s) 735, 940
BplI GAGNNNNNCTC 2 cut(s) 48, 80
BpmI CTGGAG 3 cut(s) 425, 573, 660
BsaBI GATNNNNATC 1 cut(s) 278
BsaJI CCNNGG 1 cut(s) 463
BsaWI WCCGGW 1 cut(s) 534
BsaXI ACNNNNNCTCC 2 cut(s) 865, 895
Bsc4I CCNNNNNNNGG 2 cut(s) 375, 469
Bse118I RCCGGY 1 cut(s) 534
Bse1I ACTGG 1 cut(s) 408
Bse8I GATNNNNATC 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 463
BseGI GGATG 1 cut(s) 750
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 2 cut(s) 375, 469
BseMII CTCAG 2 cut(s) 828, 891
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 4 cut(s) 352, 563, 915, 1058
BsgI GTGCAG 1 cut(s) 944
Bsh1236I CGCG 1 cut(s) 741
BshFI GGCC 3 cut(s) 175, 827, 1071
BshTI ACCGGT 1 cut(s) 534
BsiHKAI GWGCWC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 861
BsiSI CCGG 1 cut(s) 535
BslFI GGGAC 2 cut(s) 571, 787
BslI CCNNNNNNNGG 2 cut(s) 375, 469
BsmFI GGGAC 2 cut(s) 571, 787
BsnI GGCC 3 cut(s) 175, 827, 1071
BsoBI CYCGRG 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 63
Bsp1407I TGTACA 1 cut(s) 329
Bsp143I GATC 3 cut(s) 589, 907, 938
BspACI CCGC 3 cut(s) 101, 456, 741
BspANI GGCC 3 cut(s) 175, 827, 1071
BspCNI CTCAG 2 cut(s) 829, 892
BspFNI CGCG 1 cut(s) 741
BspHI TCATGA 1 cut(s) 1095
BspLI GGNNCC 2 cut(s) 533, 687
BspMI ACCTGC 1 cut(s) 966
BspPI GGATC 1 cut(s) 946
BsrFI RCCGGY 1 cut(s) 534
BsrGI TGTACA 1 cut(s) 329
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 534
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 3 cut(s) 589, 907, 938
BssT1I CCWWGG 1 cut(s) 463
Bst4CI ACNGT 2 cut(s) 162, 337
BstAUI TGTACA 1 cut(s) 329
BstC8I GCNNGC 3 cut(s) 144, 218, 999
BstDEI CTNAG 2 cut(s) 837, 900
BstENI CCTNNNNNAGG 1 cut(s) 467
BstF5I GGATG 1 cut(s) 750
BstFNI CGCG 1 cut(s) 741
BstH2I RGCGCY 1 cut(s) 998
BstHHI GCGC 1 cut(s) 997
BstKTI GATC 3 cut(s) 592, 910, 941
BstMBI GATC 3 cut(s) 589, 907, 938
BstMWI GCNNNNNNNGC 5 cut(s) 51, 93, 107, 641, 980
BstNSI RCATGY 1 cut(s) 421
BstSFI CTRYAG 1 cut(s) 1002
BstUI CGCG 1 cut(s) 741
BstV1I GCAGC 4 cut(s) 352, 563, 915, 1058
BsuRI GGCC 3 cut(s) 175, 827, 1071
BtsCI GGATG 1 cut(s) 750
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 1 cut(s) 343
BveI ACCTGC 1 cut(s) 966
Cac8I GCNNGC 3 cut(s) 144, 218, 999
CciI TCATGA 1 cut(s) 1095
CfoI GCGC 1 cut(s) 997
Cfr10I RCCGGY 1 cut(s) 534
Cfr13I GGNCC 1 cut(s) 1069
Csp6I GTAC 2 cut(s) 308, 330
CspAI ACCGGT 1 cut(s) 534
CviAII CATG 8 cut(s) 46, 125, 305, 418, 854, 935, 1013, 1096
CviQI GTAC 2 cut(s) 308, 330
DdeI CTNAG 2 cut(s) 837, 900
DpnI GATC 3 cut(s) 591, 909, 940
DpnII GATC 3 cut(s) 589, 907, 938
DraI TTTAAA 1 cut(s) 894
EciI GGCGGA 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 463
Eco147I AGGCCT 1 cut(s) 827
Eco32I GATATC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 789
Eco88I CYCGRG 1 cut(s) 861
EcoNI CCTNNNNNAGG 1 cut(s) 467
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 463
EcoT22I ATGCAT 1 cut(s) 709
ErhI CCWWGG 1 cut(s) 463
FaeI CATG 8 cut(s) 49, 128, 308, 421, 857, 938, 1016, 1099
FaqI GGGAC 2 cut(s) 571, 787
FatI CATG 8 cut(s) 45, 124, 304, 417, 853, 934, 1012, 1095
FauI CCCGC 1 cut(s) 734
FauNDI CATATG 1 cut(s) 709
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 5 cut(s) 6, 366, 577, 929, 1047
FokI GGATG 1 cut(s) 757
Fsp4HI GCNGC 5 cut(s) 6, 366, 577, 929, 1047
FspBI CTAG 1 cut(s) 311
GlaI GCGC 1 cut(s) 996
GluI GCNGC 5 cut(s) 6, 366, 577, 929, 1047
GsuI CTGGAG 3 cut(s) 425, 573, 660
HaeII RGCGCY 1 cut(s) 998
HaeIII GGCC 3 cut(s) 175, 827, 1071
HapII CCGG 1 cut(s) 535
HhaI GCGC 1 cut(s) 997
Hin1II CATG 8 cut(s) 49, 128, 308, 421, 857, 938, 1016, 1099
Hin6I GCGC 1 cut(s) 995
HinP1I GCGC 1 cut(s) 995
HincII GTYRAC 2 cut(s) 523, 655
HindII GTYRAC 2 cut(s) 523, 655
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 1 cut(s) 559
HpaI GTTAAC 1 cut(s) 655
HpaII CCGG 1 cut(s) 535
HphI GGTGA 1 cut(s) 818
Hpy166II GTNNAC 4 cut(s) 330, 494, 523, 655
Hpy188I TCNGA 2 cut(s) 273, 594
Hpy188III TCNNGA 8 cut(s) 149, 552, 639, 689, 836, 886, 1063, 1096
Hpy8I GTNNAC 4 cut(s) 330, 494, 523, 655
HpyAV CCTTC 6 cut(s) 86, 141, 445, 764, 863, 1060
HpyCH4III ACNGT 2 cut(s) 162, 337
HpyCH4V TGCA 8 cut(s) 76, 348, 707, 931, 961, 974, 1012, 1046
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 93, 107, 641, 980
HpyF3I CTNAG 2 cut(s) 837, 900
Hsp92II CATG 8 cut(s) 49, 128, 308, 421, 857, 938, 1016, 1099
HspAI GCGC 1 cut(s) 995
Ksp22I TGATCA 1 cut(s) 907
KspAI GTTAAC 1 cut(s) 655
Kzo9I GATC 3 cut(s) 589, 907, 938
LmnI GCTCC 1 cut(s) 641
Lsp1109I GCAGC 4 cut(s) 352, 563, 915, 1058
LweI GCATC 2 cut(s) 735, 940
MaeI CTAG 1 cut(s) 311
MaeIII GTNAC 2 cut(s) 806, 1078
MalI GATC 3 cut(s) 591, 909, 940
MboI GATC 3 cut(s) 589, 907, 938
MboII GAAGA 2 cut(s) 74, 1100
MfeI CAATTG 1 cut(s) 526
MhlI GDGCHC 1 cut(s) 63
MluCI AATT 5 cut(s) 169, 267, 526, 696, 722
MlyI GAGTC 1 cut(s) 553
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 6 cut(s) 74, 346, 369, 548, 591, 817
Mph1103I ATGCAT 1 cut(s) 709
MroXI GAANNNNTTC 1 cut(s) 536
MseI TTAA 5 cut(s) 168, 516, 546, 654, 893
MslI CAYNNNNRTG 2 cut(s) 663, 863
MspI CCGG 1 cut(s) 535
MunI CAATTG 1 cut(s) 526
MvnI CGCG 1 cut(s) 741
MwoI GCNNNNNNNGC 5 cut(s) 51, 93, 107, 641, 980
NdeI CATATG 1 cut(s) 709
NdeII GATC 3 cut(s) 589, 907, 938
NlaIII CATG 8 cut(s) 49, 128, 308, 421, 857, 938, 1016, 1099
NlaIV GGNNCC 2 cut(s) 533, 687
NmuCI GTSAC 2 cut(s) 806, 1078
NsiI ATGCAT 1 cut(s) 709
NspI RCATGY 1 cut(s) 421
OliI CACNNNNGTG 1 cut(s) 863
PaeR7I CTCGAG 1 cut(s) 861
PagI TCATGA 1 cut(s) 1095
PaqCI CACCTGC 1 cut(s) 966
PceI AGGCCT 1 cut(s) 827
PdmI GAANNNNTTC 1 cut(s) 536
PinAI ACCGGT 1 cut(s) 534
PkrI GCNGC 5 cut(s) 7, 367, 578, 930, 1048
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
PspN4I GGNNCC 2 cut(s) 533, 687
PspPI GGNCC 1 cut(s) 1069
PspXI VCTCGAGB 1 cut(s) 861
RsaI GTAC 2 cut(s) 309, 331
RsaNI GTAC 2 cut(s) 308, 330
RseI CAYNNNNRTG 2 cut(s) 663, 863
SaqAI TTAA 5 cut(s) 168, 516, 546, 654, 893
SatI GCNGC 5 cut(s) 6, 366, 577, 929, 1047
Sau3AI GATC 3 cut(s) 589, 907, 938
Sau96I GGNCC 1 cut(s) 1069
SchI GAGTC 1 cut(s) 553
SduI GDGCHC 1 cut(s) 63
SfaNI GCATC 2 cut(s) 735, 940
SfcI CTRYAG 1 cut(s) 1002
Sfr274I CTCGAG 1 cut(s) 861
SlaI CTCGAG 1 cut(s) 861
SmiMI CAYNNNNRTG 2 cut(s) 663, 863
SmlI CTYRAG 1 cut(s) 861
SmoI CTYRAG 1 cut(s) 861
SpeI ACTAGT 1 cut(s) 310
Sse9I AATT 5 cut(s) 169, 267, 526, 696, 722
SseBI AGGCCT 1 cut(s) 827
SsiI CCGC 3 cut(s) 101, 456, 741
SspMI CTAG 1 cut(s) 311
StuI AGGCCT 1 cut(s) 827
StyI CCWWGG 1 cut(s) 463
TaaI ACNGT 2 cut(s) 162, 337
TaqI TCGA 3 cut(s) 720, 862, 1062
TasI AATT 5 cut(s) 169, 267, 526, 696, 722
TatI WGTACW 2 cut(s) 307, 329
Tru1I TTAA 5 cut(s) 168, 516, 546, 654, 893
Tru9I TTAA 5 cut(s) 168, 516, 546, 654, 893
TscAI CASTG 1 cut(s) 350
TseFI GTSAC 2 cut(s) 806, 1078
TseI GCWGC 5 cut(s) 5, 365, 576, 928, 1046
Tsp45I GTSAC 2 cut(s) 806, 1078
TspDTI ATGAA 2 cut(s) 681, 798
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 1 cut(s) 350
XagI CCTNNNNNAGG 1 cut(s) 467
XapI RAATTY 2 cut(s) 267, 722
XceI RCATGY 1 cut(s) 421
XhoI CTCGAG 1 cut(s) 861
XmnI GAANNNNTTC 1 cut(s) 536
XspI CTAG 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.