Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011071

Physical Location & Seq

Genomic Coordinates Reverse (-)

18475193 .. 18486381

Gene Structure 11189 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1099900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGATCAGTACGGCCAACGGTATGATGCAAACGGCGTCGTCTAGCAACGCTCAGTCGCCGGGTCTCAAGACCTATTTCAAAACCCCTGAGGGAAAGTATAAGCTTCAGTACGAGAAGACGCATCCTTCGGGGCTCCTTCACTACGCCCATGGAAAAACCGTCACTCAGGCAACATTAGCAAATCTTAAGGATAAACCTGCTCCATCAACCCCGACTGCGCCATCTTCGAGCTTCAGCACTGGAAGTGGCGTACGTTCGGCTGCAGCAAGGTTGTTGGGTGGTGGTAATGGAACCCGGGCGCTTAGCTTTGTTGCAGGGAATGGAGGGGGTAAGAGTGTCAGTGCGAGTAGTAGAGTTGGTTCTTTGGTGGCTTCCAGTTCGAGTACTTCGACTTCTACTTCGAATTTTGATGGGAAAGGGAATTATTTGATCTTCAATGTGGGGGATGCGATCTTCATCAGTGATTTGAATTCTCAAGACAAGGATCCAATAAAGTCTATACATTTCAGTAACTCAAACCCTGTCTGCCATGCATTTGATCAAGACGCGAAGGATGGTCATGATTTGATTATTGGGTTGAGTTGCGGTGATGTCTACTCAGTGTCACTGAGACAGCAATTGCAGGATGTTGGAAAGAAGCTTGTAGGTGCTCAGCATTATAACAAAGATGGCTCTGTTAGTAACAGCCGTTGTACTAGTATTGCATGGGTTCCTGGTGGTGACGGTGCTTTTGTTGTTGCTCATGCCGATGGGAATCTGTATAGCAAAGATGGGGCAGGCGATTCCTCATTCCCTGTTATCAAAGATCCAGCTCAATTTTCAGTTTCGCATGCACGTTACAATAAGAGTAACCCAATTGCCAGATGGCATATCTGCCAAGGTTCAGTAAATGGTATTGCTTTCTCGACTGATGGGTCCTATTTAGCGACTGTTGGAAGAGATGGTTATCTACGTGTTTTTGACTACTCAAAAGAGCAACTTATATGTGGTGGCAAAAGTTACTATGGGGCCCTTTTATGTTGCGCTTGGAGCATGGATGGAAAATACATACTGACAGGAGGTGAAGATGATCTAGTTCAAGTTTGGAGCATGGAAGATCGGAAAGTTGTAGCATGGGGAGAGGGGCACAACTCTTGGGTTAGTGGAGTGGCATTTGATTCGTATTGGTCGTCACCAAATTCAGATGGTATGGGGGAGACTGTCATGTACCGGTTTGGCTCCGTCGGTCAGGACACACAGTTGCTTCTGTGGGACCTGGAAATGGATGAGATTGTTGTGCCATTGCGGCGATGTCCTCCTGGCGGGTCCCCCACATATAGTGCTGGAAGCCAGTCATCTCATTGGGACAATGCACTTCCAGTAGGTACTCTGCAGCCTGCTCCAAGCTTGCGAGATGTTCCAAAAATATCACCGCTGGTTGCTCACCGGGTACACACTGAACCCCTCTCTGGCGTGATGTTTACCCAAGAATCCGTTCTTACTGTCTGCCGAGAGGGGCATATAAAAGTTTGGGTGAGACCTGGGGTTTCAGAAACCCAATCAAACAACTCGTCCGAATTAAGTACCAGCTCGAAGGAAAAGCCTTTGTCCTCAGTCAAGGTTGGCAGTTCGAGTTACAAGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006355 GO:0007154 GO:0007584 GO:0008150 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031670 GO:0042221 GO:0045013 GO:0045892 GO:0045934 GO:0045990 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0061984 GO:0061985 GO:0065007 GO:0070887 GO:0071496 GO:0080090 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

542

Amino Acids

57.57

Weight (kDa)

7.22

Isoelectric Point (pI)

41.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	281 - 424	7.8e-12	WDR3 first beta-propeller domain
WDR55	PF24796	283 - 386	1.7e-07	WDR55
WD40_Prp19	PF24814	289 - 419	2.4e-09	Prp19 WD40 domain
Beta-prop_TEP1_2nd	PF25047	292 - 384	1.9e-08	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	293 - 391	1.4e-13	WDR5 beta-propeller domain
Beta-prop_EML_2	PF23414	297 - 386	8.3e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3	PF25174	340 - 424	2.5e-07	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011071)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37160 AT2G37160 AT3G53390
fragaria_vesca	FvH4_6g15710 FvH4_6g15710
malus_domestica	MD04G1099900.v1.1 MD12G1120000.v1.1
prunus_persica	Prupe.6G238200_v2.0.a1
pyrus_communis	pycom04g09500 pycom12g11890
rosa_chinensis	RchiOBHm_Chr3g0469411
rosa_laevigata	RLG00000024351
rosa_multiflora	Rmu_sc0005274.1_g000009
rosa_roxburghii	Rroxscaffold_6G00411910
rosa_rugosa	Rorug03G0101600
rosa_samantha	Rh3AG150600 Rh3BG174300 Rh3CG165300 Rh3DG201100
rosa_wichuraiana	Rw3G014200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	660
AasI	GACNNNNNNGTC	1 cut(s)	913
Acc36I	ACCTGC	1 cut(s)	205
AccI	GTMKAC	1 cut(s)	594
AccII	CGCG	1 cut(s)	548
AciI	CCGC	4 cut(s)	585, 1285, 1302, 1412
AclWI	GGATC	3 cut(s)	479, 492, 800
AcoI	YGGCCR	1 cut(s)	12
AcsI	RAATTY	3 cut(s)	403, 469, 1177
AcuI	CTGAAG	2 cut(s)	89, 217
AcyI	GRCGYC	1 cut(s)	35
AfaI	GTAC	9 cut(s)	10, 110, 252, 385, 694, 1208, 1366, 1431, 1564
AfiI	CCNNNNNNNGG	3 cut(s)	1261, 1301, 1448
AflII	CTTAAG	1 cut(s)	185
AflIII	ACRYGT	1 cut(s)	952
AgeI	ACCGGT	1 cut(s)	1209
AgsI	TTSAA	4 cut(s)	79, 436, 469, 1079
AhlI	ACTAGT	1 cut(s)	695
AjnI	CCWGG	4 cut(s)	712, 1254, 1297, 1519
AjuI	GAANNNNNNNTTGG	2 cut(s)	1458, 1490
AluBI	AGCT	7 cut(s)	103, 231, 306, 640, 812, 1386, 1569
AluI	AGCT	7 cut(s)	103, 231, 306, 640, 812, 1386, 1569
Alw21I	GWGCWC	1 cut(s)	652
Alw26I	GTCTC	4 cut(s)	68, 604, 1190, 1510
AlwI	GGATC	3 cut(s)	479, 492, 800
Ama87I	CYCGRG	1 cut(s)	294
AoxI	GGCC	2 cut(s)	12, 1008
ApaI	GGGCCC	1 cut(s)	1012
ApeKI	GCWGC	3 cut(s)	260, 263, 1372
ApoI	RAATTY	3 cut(s)	403, 469, 1177
ArsI	GACNNNNNNTTYG	8 cut(s)	109, 141, 382, 414, 713, 745, 1535, 1567
AsiGI	ACCGGT	1 cut(s)	1209
Asp700I	GAANNNNTTC	1 cut(s)	1473
AspLEI	GCGC	3 cut(s)	220, 301, 1025
AspS9I	GGNCC	5 cut(s)	915, 1008, 1009, 1252, 1305
AsuC2I	CCSGG	4 cut(s)	60, 295, 296, 1427
AsuHPI	GGTGA	7 cut(s)	599, 731, 1073, 1164, 1401, 1415, 1525
AsuII	TTCGAA	1 cut(s)	401
AvaI	CYCGRG	1 cut(s)	294
AvaII	GGWCC	3 cut(s)	915, 1252, 1305
AxyI	CCTNAGG	1 cut(s)	87
BaeGI	GKGCMC	2 cut(s)	1012, 1128
BamHI	GGATCC	1 cut(s)	484
BanII	GRGCYC	2 cut(s)	135, 1012
BarI	GAAGNNNNNNTAC	2 cut(s)	376, 408
BbsI	GAAGAC	1 cut(s)	122
Bbv12I	GWGCWC	1 cut(s)	652
BbvI	GCAGC	3 cut(s)	247, 275, 1384
BceAI	ACGGC	3 cut(s)	27, 48, 672
BcgI	CGANNNNNNTGC	2 cut(s)	1140, 1174
BciT130I	CCWGG	4 cut(s)	714, 1256, 1299, 1521
BclI	TGATCA	2 cut(s)	3, 538
BcnI	CCSGG	4 cut(s)	60, 295, 296, 1427
BcoDI	GTCTC	4 cut(s)	68, 604, 1190, 1510
BcuI	ACTAGT	1 cut(s)	695
BfaI	CTAG	3 cut(s)	42, 696, 1073
BfmI	CTRYAG	2 cut(s)	261, 1370
BfoI	RGCGCY	1 cut(s)	302
BfrI	CTTAAG	1 cut(s)	185
BfuAI	ACCTGC	1 cut(s)	205
BglI	GCCNNNNNGGC	1 cut(s)	1285
BisI	GCNGC	4 cut(s)	261, 264, 1286, 1373
BlpI	GCTNAGC	2 cut(s)	302, 651
BlsI	GCNGC	4 cut(s)	262, 265, 1287, 1374
BmcAI	AGTACT	1 cut(s)	385
Bme1390I	CCNGG	8 cut(s)	60, 295, 296, 714, 1256, 1299, 1427, 1521
Bme18I	GGWCC	3 cut(s)	915, 1252, 1305
BmeT110I	CYCGRG	1 cut(s)	294
BmgT120I	GGNCC	5 cut(s)	915, 1008, 1009, 1252, 1305
BmrFI	CCNGG	8 cut(s)	60, 295, 296, 714, 1256, 1299, 1427, 1521
BmsI	GCATC	3 cut(s)	15, 130, 436
BpiI	GAAGAC	1 cut(s)	122
Bpu1102I	GCTNAGC	2 cut(s)	302, 651
Bpu14I	TTCGAA	1 cut(s)	401
BpuEI	CTTGAG	2 cut(s)	50, 459
BpuMI	CCSGG	4 cut(s)	60, 295, 296, 1427
BsaAI	YACGTR	1 cut(s)	953
BsaBI	GATNNNNATC	3 cut(s)	455, 753, 945
BsaHI	GRCGYC	1 cut(s)	35
BsaI	GGTCTC	2 cut(s)	68, 1510
BsaJI	CCNNGG	4 cut(s)	148, 294, 877, 1520
BsaWI	WCCGGW	1 cut(s)	1209
Bsc4I	CCNNNNNNNGG	3 cut(s)	1261, 1301, 1448
Bse118I	RCCGGY	1 cut(s)	1209
Bse1I	ACTGG	4 cut(s)	244, 375, 1330, 1358
Bse21I	CCTNAGG	1 cut(s)	87
Bse3DI	GCAATG	1 cut(s)	1280
Bse8I	GATNNNNATC	3 cut(s)	455, 753, 945
BseBI	CCWGG	4 cut(s)	714, 1256, 1299, 1521
BseDI	CCNNGG	4 cut(s)	148, 294, 877, 1520
BseGI	GGATG	6 cut(s)	121, 451, 559, 631, 1042, 1270
BseJI	GATNNNNATC	3 cut(s)	455, 753, 945
BseLI	CCNNNNNNNGG	3 cut(s)	1261, 1301, 1448
BseMI	GCAATG	1 cut(s)	1280
BseMII	CTCAG	7 cut(s)	65, 78, 179, 599, 612, 665, 1605
BseNI	ACTGG	4 cut(s)	244, 375, 1330, 1358
BseSI	GKGCMC	2 cut(s)	1012, 1128
BseXI	GCAGC	3 cut(s)	247, 275, 1384
Bsh1236I	CGCG	1 cut(s)	548
BshFI	GGCC	2 cut(s)	14, 1010
BshTI	ACCGGT	1 cut(s)	1209
BsiHKAI	GWGCWC	1 cut(s)	652
BsiHKCI	CYCGRG	1 cut(s)	294
BsiSI	CCGG	4 cut(s)	59, 295, 1210, 1426
BsiWI	CGTACG	1 cut(s)	250
BslFI	GGGAC	3 cut(s)	1265, 1291, 1358
BslI	CCNNNNNNNGG	3 cut(s)	1261, 1301, 1448
BsmAI	GTCTC	4 cut(s)	68, 604, 1190, 1510
BsmFI	GGGAC	3 cut(s)	1265, 1291, 1358
BsnI	GGCC	2 cut(s)	14, 1010
Bso31I	GGTCTC	2 cut(s)	68, 1510
BsoBI	CYCGRG	1 cut(s)	294
Bsp119I	TTCGAA	1 cut(s)	401
Bsp120I	GGGCCC	1 cut(s)	1008
Bsp1286I	GDGCHC	4 cut(s)	135, 652, 1012, 1128
Bsp143I	GATC	8 cut(s)	3, 429, 450, 484, 538, 805, 1069, 1096
Bsp1720I	GCTNAGC	2 cut(s)	302, 651
Bsp19I	CCATGG	1 cut(s)	148
BspACI	CCGC	4 cut(s)	585, 1285, 1302, 1412
BspANI	GGCC	2 cut(s)	14, 1010
BspCNI	CTCAG	7 cut(s)	64, 79, 178, 600, 611, 664, 1604
BspFNI	CGCG	1 cut(s)	548
BspHI	TCATGA	1 cut(s)	559
BspMAI	CTGCAG	2 cut(s)	265, 1374
BspMI	ACCTGC	1 cut(s)	205
BspPI	GGATC	3 cut(s)	479, 492, 800
BspT104I	TTCGAA	1 cut(s)	401
BspTI	CTTAAG	1 cut(s)	185
BspTNI	GGTCTC	2 cut(s)	68, 1510
BsrDI	GCAATG	1 cut(s)	1280
BsrFI	RCCGGY	1 cut(s)	1209
BsrI	ACTGG	4 cut(s)	244, 375, 1330, 1358
BssAI	RCCGGY	1 cut(s)	1209
BssECI	CCNNGG	4 cut(s)	148, 294, 877, 1520
BssMI	GATC	8 cut(s)	3, 429, 450, 484, 538, 805, 1069, 1096
BssNI	GRCGYC	1 cut(s)	35
BssT1I	CCWWGG	2 cut(s)	148, 877
Bst2UI	CCWGG	4 cut(s)	714, 1256, 1299, 1521
Bst4CI	ACNGT	7 cut(s)	20, 160, 725, 931, 1201, 1239, 1483
Bst6I	CTCTTC	1 cut(s)	931
BstACI	GRCGYC	1 cut(s)	35
BstAFI	CTTAAG	1 cut(s)	185
BstBAI	YACGTR	1 cut(s)	953
BstBI	TTCGAA	1 cut(s)	401
BstC8I	GCNNGC	4 cut(s)	778, 831, 1377, 1388
BstDEI	CTNAG	8 cut(s)	51, 87, 165, 302, 598, 608, 651, 1591
BstDSI	CCRYGG	1 cut(s)	148
BstF5I	GGATG	6 cut(s)	121, 451, 559, 631, 1042, 1270
BstFNI	CGCG	1 cut(s)	548
BstH2I	RGCGCY	1 cut(s)	302
BstHHI	GCGC	3 cut(s)	220, 301, 1025
BstKTI	GATC	8 cut(s)	6, 432, 453, 487, 541, 808, 1072, 1099
BstMAI	GTCTC	4 cut(s)	68, 604, 1190, 1510
BstMBI	GATC	8 cut(s)	3, 429, 450, 484, 538, 805, 1069, 1096
BstMWI	GCNNNNNNNGC	3 cut(s)	176, 1029, 1285
BstNI	CCWGG	4 cut(s)	714, 1256, 1299, 1521
BstNSI	RCATGY	1 cut(s)	833
BstSCI	CCNGG	8 cut(s)	58, 293, 294, 712, 1254, 1297, 1425, 1519
BstSFI	CTRYAG	2 cut(s)	261, 1370
BstSLI	GKGCMC	2 cut(s)	1012, 1128
BstUI	CGCG	1 cut(s)	548
BstV1I	GCAGC	3 cut(s)	247, 275, 1384
BstV2I	GAAGAC	1 cut(s)	122
BstX2I	RGATCY	2 cut(s)	484, 805
BstYI	RGATCY	2 cut(s)	484, 805
Bsu36I	CCTNAGG	1 cut(s)	87
BsuRI	GGCC	2 cut(s)	14, 1010
BtgI	CCRYGG	1 cut(s)	148
BtgZI	GCGATG	1 cut(s)	1303
BtsCI	GGATG	6 cut(s)	121, 451, 559, 631, 1042, 1270
BtsIMutI	CAGTG	6 cut(s)	237, 346, 466, 605, 606, 1434
BveI	ACCTGC	1 cut(s)	205
Cac8I	GCNNGC	4 cut(s)	778, 831, 1377, 1388
CciI	TCATGA	1 cut(s)	559
CfoI	GCGC	3 cut(s)	220, 301, 1025
Cfr10I	RCCGGY	1 cut(s)	1209
Cfr13I	GGNCC	5 cut(s)	915, 1008, 1009, 1252, 1305
Cfr9I	CCCGGG	1 cut(s)	294
CseI	GACGC	3 cut(s)	24, 127, 554
Csp6I	GTAC	9 cut(s)	9, 109, 251, 384, 693, 1207, 1365, 1430, 1563
CspAI	ACCGGT	1 cut(s)	1209
CviQI	GTAC	9 cut(s)	9, 109, 251, 384, 693, 1207, 1365, 1430, 1563
DdeI	CTNAG	8 cut(s)	51, 87, 165, 302, 598, 608, 651, 1591
DpnI	GATC	8 cut(s)	5, 431, 452, 486, 540, 807, 1071, 1098
DpnII	GATC	8 cut(s)	3, 429, 450, 484, 538, 805, 1069, 1096
DrdI	GACNNNNNNGTC	1 cut(s)	913
DseDI	GACNNNNNNGTC	1 cut(s)	913
EaeI	YGGCCR	1 cut(s)	12
Eam1104I	CTCTTC	1 cut(s)	931
EarI	CTCTTC	1 cut(s)	931
Eco130I	CCWWGG	2 cut(s)	148, 877
Eco24I	GRGCYC	2 cut(s)	135, 1012
Eco31I	GGTCTC	2 cut(s)	68, 1510
Eco47I	GGWCC	3 cut(s)	915, 1252, 1305
Eco57I	CTGAAG	2 cut(s)	89, 217
Eco81I	CCTNAGG	1 cut(s)	87
Eco88I	CYCGRG	1 cut(s)	294
EcoO109I	RGGNCCY	5 cut(s)	915, 1008, 1009, 1252, 1305
EcoRI	GAATTC	1 cut(s)	469
EcoRII	CCWGG	4 cut(s)	712, 1254, 1297, 1519
EcoT14I	CCWWGG	2 cut(s)	148, 877
EcoT22I	ATGCAT	1 cut(s)	535
EcoT38I	GRGCYC	2 cut(s)	135, 1012
ErhI	CCWWGG	2 cut(s)	148, 877
FalI	AAGNNNNNCTT	2 cut(s)	963, 995
FaqI	GGGAC	3 cut(s)	1265, 1291, 1358
FauI	CCCGC	1 cut(s)	1295
FbaI	TGATCA	2 cut(s)	3, 538
FblI	GTMKAC	1 cut(s)	594
Fnu4HI	GCNGC	4 cut(s)	261, 264, 1286, 1373
FokI	GGATG	6 cut(s)	108, 458, 566, 638, 1049, 1277
FriOI	GRGCYC	2 cut(s)	135, 1012
Fsp4HI	GCNGC	4 cut(s)	261, 264, 1286, 1373
FspBI	CTAG	3 cut(s)	42, 696, 1073
GlaI	GCGC	3 cut(s)	219, 300, 1024
GluI	GCNGC	4 cut(s)	261, 264, 1286, 1373
HaeII	RGCGCY	1 cut(s)	302
HaeIII	GGCC	2 cut(s)	14, 1010
HapII	CCGG	4 cut(s)	59, 295, 1210, 1426
HgaI	GACGC	3 cut(s)	24, 127, 554
HhaI	GCGC	3 cut(s)	220, 301, 1025
Hin1I	GRCGYC	1 cut(s)	35
Hin6I	GCGC	3 cut(s)	218, 299, 1023
HinP1I	GCGC	3 cut(s)	218, 299, 1023
HindIII	AAGCTT	3 cut(s)	101, 638, 1384
HinfI	GANTC	4 cut(s)	754, 782, 1157, 1469
HpaII	CCGG	4 cut(s)	59, 295, 1210, 1426
HphI	GGTGA	7 cut(s)	599, 731, 1073, 1164, 1401, 1415, 1525
Hpy166II	GTNNAC	3 cut(s)	595, 1432, 1461
Hpy188I	TCNGA	4 cut(s)	1101, 1183, 1531, 1555
Hpy188III	TCNNGA	6 cut(s)	67, 476, 542, 560, 904, 1229
Hpy8I	GTNNAC	3 cut(s)	595, 1432, 1461
Hpy99I	CGWCG	2 cut(s)	40, 1226
HpyAV	CCTTC	4 cut(s)	135, 146, 544, 1567
HpyCH4III	ACNGT	7 cut(s)	20, 160, 725, 931, 1201, 1239, 1483
HpyCH4IV	ACGT	3 cut(s)	253, 835, 952
HpyCH4V	TGCA	9 cut(s)	28, 263, 314, 533, 622, 704, 833, 1352, 1372
HpyF10VI	GCNNNNNNNGC	3 cut(s)	176, 1029, 1285
HpyF3I	CTNAG	8 cut(s)	51, 87, 165, 302, 598, 608, 651, 1591
HpySE526I	ACGT	3 cut(s)	253, 835, 952
Hsp92I	GRCGYC	1 cut(s)	35
HspAI	GCGC	3 cut(s)	218, 299, 1023
KflI	GGGWCCC	1 cut(s)	1305
Ksp22I	TGATCA	2 cut(s)	3, 538
Kzo9I	GATC	8 cut(s)	3, 429, 450, 484, 538, 805, 1069, 1096
LmnI	GCTCC	6 cut(s)	138, 205, 1029, 1086, 1223, 1384
Lsp1109I	GCAGC	3 cut(s)	247, 275, 1384
LweI	GCATC	3 cut(s)	15, 130, 436
MaeI	CTAG	3 cut(s)	42, 696, 1073
MaeII	ACGT	3 cut(s)	253, 835, 952
MalI	GATC	8 cut(s)	5, 431, 452, 486, 540, 807, 1071, 1098
MboI	GATC	8 cut(s)	3, 429, 450, 484, 538, 805, 1069, 1096
MboII	GAAGA	7 cut(s)	127, 216, 424, 445, 948, 1076, 1106
MfeI	CAATTG	2 cut(s)	617, 855
MflI	RGATCY	2 cut(s)	484, 805
MhlI	GDGCHC	4 cut(s)	135, 652, 1012, 1128
MluCI	AATT	8 cut(s)	403, 421, 469, 617, 815, 855, 1177, 1556
MmeI	TCCRAC	2 cut(s)	610, 913
MnlI	CCTC	9 cut(s)	82, 317, 796, 1052, 1114, 1305, 1454, 1486, 1600
Mph1103I	ATGCAT	1 cut(s)	535
MroXI	GAANNNNTTC	1 cut(s)	1473
MseI	TTAA	2 cut(s)	186, 1559
MslI	CAYNNNNRTG	1 cut(s)	747
MspA1I	CMGCKG	1 cut(s)	1414
MspCI	CTTAAG	1 cut(s)	185
MspI	CCGG	4 cut(s)	59, 295, 1210, 1426
MspR9I	CCNGG	8 cut(s)	60, 295, 296, 714, 1256, 1299, 1427, 1521
MunI	CAATTG	2 cut(s)	617, 855
MvaI	CCWGG	4 cut(s)	714, 1256, 1299, 1521
MvnI	CGCG	1 cut(s)	548
MwoI	GCNNNNNNNGC	3 cut(s)	176, 1029, 1285
NciI	CCSGG	4 cut(s)	60, 295, 296, 1427
NcoI	CCATGG	1 cut(s)	148
NdeII	GATC	8 cut(s)	3, 429, 450, 484, 538, 805, 1069, 1096
NmeAIII	GCCGAG	1 cut(s)	1514
NmuCI	GTSAC	4 cut(s)	160, 603, 719, 1170
NsiI	ATGCAT	1 cut(s)	535
NspI	RCATGY	1 cut(s)	833
NspV	TTCGAA	1 cut(s)	401
PaeI	GCATGC	1 cut(s)	833
PagI	TCATGA	1 cut(s)	559
PcsI	WCGNNNNNNNCGW	2 cut(s)	386, 1166
PdmI	GAANNNNTTC	1 cut(s)	1473
PfeI	GAWTC	4 cut(s)	754, 782, 1157, 1469
Pfl23II	CGTACG	1 cut(s)	250
PinAI	ACCGGT	1 cut(s)	1209
PkrI	GCNGC	4 cut(s)	262, 265, 1287, 1374
Ppu21I	YACGTR	1 cut(s)	953
PpuMI	RGGWCCY	3 cut(s)	915, 1252, 1305
PsiI	TTATAA	1 cut(s)	660
Psp5II	RGGWCCY	3 cut(s)	915, 1252, 1305
Psp6I	CCWGG	4 cut(s)	712, 1254, 1297, 1519
PspGI	CCWGG	4 cut(s)	712, 1254, 1297, 1519
PspLI	CGTACG	1 cut(s)	250
PspOMI	GGGCCC	1 cut(s)	1008
PspPI	GGNCC	5 cut(s)	915, 1008, 1009, 1252, 1305
PspPPI	RGGWCCY	3 cut(s)	915, 1252, 1305
PsrI	GAACNNNNNNTAC	2 cut(s)	343, 375
PstI	CTGCAG	2 cut(s)	265, 1374
PsuI	RGATCY	2 cut(s)	484, 805
RsaI	GTAC	9 cut(s)	10, 110, 252, 385, 694, 1208, 1366, 1431, 1564
RsaNI	GTAC	9 cut(s)	9, 109, 251, 384, 693, 1207, 1365, 1430, 1563
RseI	CAYNNNNRTG	1 cut(s)	747
SaqAI	TTAA	2 cut(s)	186, 1559
SatI	GCNGC	4 cut(s)	261, 264, 1286, 1373
Sau3AI	GATC	8 cut(s)	3, 429, 450, 484, 538, 805, 1069, 1096
Sau96I	GGNCC	5 cut(s)	915, 1008, 1009, 1252, 1305
ScaI	AGTACT	1 cut(s)	385
ScrFI	CCNGG	8 cut(s)	60, 295, 296, 714, 1256, 1299, 1427, 1521
SduI	GDGCHC	4 cut(s)	135, 652, 1012, 1128
SfaNI	GCATC	3 cut(s)	15, 130, 436
SfcI	CTRYAG	2 cut(s)	261, 1370
SfuI	TTCGAA	1 cut(s)	401
SinI	GGWCC	3 cut(s)	915, 1252, 1305
SmaI	CCCGGG	1 cut(s)	296
SmiMI	CAYNNNNRTG	1 cut(s)	747
SmlI	CTYRAG	3 cut(s)	65, 185, 474
SmoI	CTYRAG	3 cut(s)	65, 185, 474
SpeI	ACTAGT	1 cut(s)	695
SphI	GCATGC	1 cut(s)	833
Sse9I	AATT	8 cut(s)	403, 421, 469, 617, 815, 855, 1177, 1556
SsiI	CCGC	4 cut(s)	585, 1285, 1302, 1412
SspMI	CTAG	3 cut(s)	42, 696, 1073
StyD4I	CCNGG	8 cut(s)	58, 293, 294, 712, 1254, 1297, 1425, 1519
StyI	CCWWGG	2 cut(s)	148, 877
TaaI	ACNGT	7 cut(s)	20, 160, 725, 931, 1201, 1239, 1483
TaiI	ACGT	3 cut(s)	256, 838, 955
TaqI	TCGA	7 cut(s)	227, 380, 389, 401, 905, 1571, 1610
TaqII	GACCGA	1 cut(s)	1214
TasI	AATT	8 cut(s)	403, 421, 469, 617, 815, 855, 1177, 1556
TatI	WGTACW	2 cut(s)	383, 692
TauI	GCSGC	1 cut(s)	1288
TfiI	GAWTC	4 cut(s)	754, 782, 1157, 1469
Tru1I	TTAA	2 cut(s)	186, 1559
Tru9I	TTAA	2 cut(s)	186, 1559
TscAI	CASTG	6 cut(s)	244, 346, 466, 606, 612, 1441
TseFI	GTSAC	4 cut(s)	160, 603, 719, 1170
TseI	GCWGC	3 cut(s)	260, 263, 1372
Tsp45I	GTSAC	4 cut(s)	160, 603, 719, 1170
TspDTI	ATGAA	1 cut(s)	445
TspGWI	ACGGA	2 cut(s)	1210, 1462
TspMI	CCCGGG	1 cut(s)	294
TspRI	CASTG	6 cut(s)	244, 346, 466, 606, 612, 1441
Vha464I	CTTAAG	1 cut(s)	185
VpaK11BI	GGWCC	3 cut(s)	915, 1252, 1305
XapI	RAATTY	3 cut(s)	403, 469, 1177
XceI	RCATGY	1 cut(s)	833
XcmI	CCANNNNNNNNNTGG	1 cut(s)	861
XmaI	CCCGGG	1 cut(s)	294
XmiI	GTMKAC	1 cut(s)	594
XmnI	GAANNNNTTC	1 cut(s)	1473
XspI	CTAG	3 cut(s)	42, 696, 1073
ZrmI	AGTACT	1 cut(s)	385
Zsp2I	ATGCAT	1 cut(s)	535

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.