Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0012177

Physical Location & Seq

Genomic Coordinates Reverse (-)

20765611 .. 20772364

Gene Structure 6754 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1121900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGTATCTCTGCGTTCCTCAATCAGTCTCCGTCAAATTCCCAGCAATCCCTTGTTTCGGTTCGTCTAGACGGGACTTCAAGATTCCCGCTATTTCCATGTCTGCCATTGAAGTCTCCCGTCACATTCCGGCAGCCCCCATTTTTCTGCCTGAAGGCCCTTGGAAGCAGATTCCCGGTGGAGTTACTGCTGCAGAAGGATTCAAAGCTGCCGGATTATATGGCGGTCTTCGTGCCATAGGCGACAAGCCTGATCTCGCTCTTGTTACTTGTGATGTGGATGCCACATCTGCTGGATCGTTTACCATGAATGTGGTAGCAGCTGCGCCGGTGTTGTATTGCAAATCAGTATTACACACTTCAGAAACGGCGCGAGCAGTTTTGATCAATGCTGGTCAAGCCAATGCAGCAACGGGTGATGCGGGATATCAAGACGTGATAGAGTGCTCAAGCAGCCTTGCTGAGCTACTTCAATTGAGACAAGAGGAAGTGCTGATTGAATCGACTGGTGTGATTGGTCAAAGGATAAAAAAGGAAGCACTTCTAAAATCTCTTCCAAAACTGGTTAATTCACTATCATCGTCAACTGAGGGGGCAGATGCTGCAGCAGTAGCAATCACAACAACCGACCTTGTTAGCAAGAGTGTGGCAATTCAGTCTCAGGTTGGTGGGATAAATATAAGAGTCGGGGGAATGGCAAAAGGTTCCGGGATGATCCACCCAAATATGGCTACCATGTTGGGGGTAGTAACAACTGATGCGATGGTTAGTAGTGATGTTTGGAGAAAAATGGTTCAAATTGCTGTCAACCGGAGCTTCAACCAAATAACAGTAGATGGAGATACAAGCACTAATGATACTGTCATTGCGTTAGCTAGTGGATTGTCTGGAACCAAGATTTCTTCCTACAATAGTAATGAAGCAGTCCAACTTCAAGCGTGCCTTGATGTGGTAATGCAAGGTCTTGCGAAATCAATAGCTTGGGACGGAGAAGGAGCAACATGCCTAATTGAGGTCACAGTGACTGGTGCAGACATTGAGGCTGAAGCAGCAAAAATAGCACGGTCGGTGGCATCTTCTTCACTAGTAAAGGCTGCAATATATGGCAGAGACCCAAACTGGGGGCGCATTGCTGCTGCTGCAGGCTATGCAGGAGTACCCTTCGACCAAAGCAAGCTCCAAGTATTGCTAGGGGATATTCTGCTCATGGATCGTGGGGAACCACAATTATTCGACAGGAGTGCGGCTAGTGACTACCTCAAGAAGGCTGGTGAGATCCATGGCACGGTTGTACTTAGTGTATCCGTAGGTGACGGCCCTGGGCGAGGTCAAGCATGGGGTTGTGATCTAAGTTATGATTATGTTAAAATTAATGCCGAGTACACAACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004042 GO:0004358 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006525 GO:0006526 GO:0006591 GO:0006592 GO:0006807 GO:0008080 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016407 GO:0016410 GO:0016740 GO:0016746 GO:0016747 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00220 ko01100 ko01110 ko01130 ko01210 ko01230 map00220 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

ArgJ

Protein Analysis

463

Amino Acids

48.1

Weight (kDa)

5.2

Isoelectric Point (pI)

39.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ArgJ	PF01960	69 - 462	1.4e-136	ArgJ family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012177)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37500 AT2G37500
fragaria_vesca	FvH4_6g14300 FvH4_6g14300
malus_domestica	MD04G1121900.v1.1 MD12G1138500.v1.1
prunus_persica	Prupe.6G250400_v2.0.a1 Prupe.6G250400_v2.0.a1
pyrus_communis	pycom04g11050 pycom12g13250
rosa_chinensis	RchiOBHm_Chr3g0467271
rosa_multiflora	Rmu_sc0036365.1_g000001
rosa_roxburghii	Rroxscaffold_6G00413760
rosa_rugosa	Rorug03G0086400
rosa_samantha	Rh3AG134700 Rh3BG156000 Rh3CG155600 Rh3DG156800
rosa_wichuraiana	Rw3G012750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	370
AciI	CCGC	4 cut(s)	87, 222, 419, 1241
AclWI	GGATC	4 cut(s)	301, 706, 1215, 1267
AcsI	RAATTY	1 cut(s)	35
AcuI	CTGAAG	3 cut(s)	171, 342, 1062
AfaI	GTAC	3 cut(s)	1155, 1290, 1379
AgsI	TTSAA	8 cut(s)	79, 110, 202, 470, 497, 794, 817, 932
AhlI	ACTAGT	1 cut(s)	1081
AjiI	CACGTC	1 cut(s)	433
AjnI	CCWGG	1 cut(s)	1315
AluBI	AGCT	7 cut(s)	206, 320, 463, 813, 872, 977, 1174
AluI	AGCT	7 cut(s)	206, 320, 463, 813, 872, 977, 1174
Alw21I	GWGCWC	1 cut(s)	446
Alw26I	GTCTC	5 cut(s)	31, 118, 469, 660, 1101
AlwI	GGATC	4 cut(s)	301, 706, 1215, 1267
AlwNI	CAGNNNCTG	2 cut(s)	599, 1022
AoxI	GGCC	2 cut(s)	154, 1312
ApoI	RAATTY	1 cut(s)	35
AseI	ATTAAT	1 cut(s)	1368
Asp700I	GAANNNNTTC	1 cut(s)	537
AspLEI	GCGC	3 cut(s)	325, 370, 1125
AspS9I	GGNCC	2 cut(s)	155, 1313
AsuC2I	CCSGG	2 cut(s)	174, 706
AsuHPI	GGTGA	3 cut(s)	425, 1280, 1319
BbsI	GAAGAC	1 cut(s)	218
Bbv12I	GWGCWC	1 cut(s)	446
BccI	CCATC	2 cut(s)	754, 827
BceAI	ACGGC	2 cut(s)	381, 1327
BcgI	CGANNNNNNTGC	2 cut(s)	1220, 1254
BciT130I	CCWGG	1 cut(s)	1317
BciVI	GTATCC	1 cut(s)	1309
BclI	TGATCA	1 cut(s)	381
BcnI	CCSGG	2 cut(s)	174, 706
BcoDI	GTCTC	5 cut(s)	31, 118, 469, 660, 1101
BcuI	ACTAGT	1 cut(s)	1081
BfaI	CTAG	5 cut(s)	66, 873, 1082, 1187, 1245
BfmI	CTRYAG	3 cut(s)	189, 600, 1137
BfuI	GTATCC	1 cut(s)	1309
BlpI	GCTNAGC	1 cut(s)	459
Bme1390I	CCNGG	3 cut(s)	174, 706, 1317
BmgBI	CACGTC	1 cut(s)	433
BmgT120I	GGNCC	2 cut(s)	155, 1313
BmiI	GGNNCC	3 cut(s)	703, 889, 1218
BmrFI	CCNGG	3 cut(s)	174, 706, 1317
BmrI	ACTGGG	1 cut(s)	1126
BmsI	GCATC	5 cut(s)	268, 406, 586, 745, 1079
BmuI	ACTGGG	1 cut(s)	1126
BpiI	GAAGAC	1 cut(s)	218
Bpu1102I	GCTNAGC	1 cut(s)	459
BpuEI	CTTGAG	2 cut(s)	430, 1241
BpuMI	CCSGG	2 cut(s)	174, 706
BsaI	GGTCTC	1 cut(s)	1101
BsaJI	CCNNGG	4 cut(s)	158, 1276, 1315, 1316
BsaWI	WCCGGW	1 cut(s)	807
Bse118I	RCCGGY	1 cut(s)	325
Bse1I	ACTGG	4 cut(s)	508, 564, 1027, 1121
Bse3DI	GCAATG	2 cut(s)	861, 1125
BseBI	CCWGG	1 cut(s)	1317
BseDI	CCNNGG	4 cut(s)	158, 1276, 1315, 1316
BseGI	GGATG	2 cut(s)	283, 714
BseMI	GCAATG	2 cut(s)	861, 1125
BseMII	CTCAG	3 cut(s)	450, 576, 671
BseNI	ACTGG	4 cut(s)	508, 564, 1027, 1121
BseYI	CCCAGC	1 cut(s)	40
BsgI	GTGCAG	1 cut(s)	1047
Bsh1236I	CGCG	1 cut(s)	370
Bsh1285I	CGRYCG	1 cut(s)	1064
BshFI	GGCC	2 cut(s)	156, 1314
BsiEI	CGRYCG	1 cut(s)	1064
BsiHKAI	GWGCWC	1 cut(s)	446
BsiSI	CCGG	6 cut(s)	128, 174, 210, 326, 705, 808
BslFI	GGGAC	2 cut(s)	86, 995
BsmAI	GTCTC	5 cut(s)	31, 118, 469, 660, 1101
BsmFI	GGGAC	2 cut(s)	86, 995
BsnI	GGCC	2 cut(s)	156, 1314
Bso31I	GGTCTC	1 cut(s)	1101
Bsp1286I	GDGCHC	1 cut(s)	446
Bsp143I	GATC	7 cut(s)	250, 293, 381, 711, 1207, 1272, 1342
Bsp1720I	GCTNAGC	1 cut(s)	459
Bsp19I	CCATGG	1 cut(s)	1276
BspACI	CCGC	4 cut(s)	87, 222, 419, 1241
BspANI	GGCC	2 cut(s)	156, 1314
BspCNI	CTCAG	3 cut(s)	451, 577, 670
BspFNI	CGCG	1 cut(s)	370
BspLI	GGNNCC	3 cut(s)	703, 889, 1218
BspMAI	CTGCAG	3 cut(s)	193, 604, 1141
BspPI	GGATC	4 cut(s)	301, 706, 1215, 1267
BspTNI	GGTCTC	1 cut(s)	1101
BsrDI	GCAATG	2 cut(s)	861, 1125
BsrFI	RCCGGY	1 cut(s)	325
BsrI	ACTGG	4 cut(s)	508, 564, 1027, 1121
BssAI	RCCGGY	1 cut(s)	325
BssECI	CCNNGG	4 cut(s)	158, 1276, 1315, 1316
BssMI	GATC	7 cut(s)	250, 293, 381, 711, 1207, 1272, 1342
BssT1I	CCWWGG	2 cut(s)	158, 1276
Bst2UI	CCWGG	1 cut(s)	1317
Bst4CI	ACNGT	5 cut(s)	829, 859, 1018, 1062, 1285
Bst6I	CTCTTC	1 cut(s)	555
BstAPI	GCANNNNNTGC	2 cut(s)	599, 1145
BstC8I	GCNNGC	4 cut(s)	372, 937, 1141, 1172
BstDEI	CTNAG	5 cut(s)	459, 585, 657, 1292, 1346
BstDSI	CCRYGG	1 cut(s)	1276
BstENI	CCTNNNNNAGG	3 cut(s)	1007, 1259, 1320
BstF5I	GGATG	2 cut(s)	283, 714
BstFNI	CGCG	1 cut(s)	370
BstHHI	GCGC	3 cut(s)	325, 370, 1125
BstKTI	GATC	7 cut(s)	253, 296, 384, 714, 1210, 1275, 1345
BstMAI	GTCTC	5 cut(s)	31, 118, 469, 660, 1101
BstMBI	GATC	7 cut(s)	250, 293, 381, 711, 1207, 1272, 1342
BstMCI	CGRYCG	1 cut(s)	1064
BstNI	CCWGG	1 cut(s)	1317
BstNSI	RCATGY	1 cut(s)	1002
BstSCI	CCNGG	3 cut(s)	172, 704, 1315
BstSFI	CTRYAG	3 cut(s)	189, 600, 1137
BstUI	CGCG	1 cut(s)	370
BstV2I	GAAGAC	1 cut(s)	218
BstX2I	RGATCY	1 cut(s)	1272
BstXI	CCANNNNNNTGG	1 cut(s)	310
BstYI	RGATCY	1 cut(s)	1272
BsuI	GTATCC	1 cut(s)	1309
BsuRI	GGCC	2 cut(s)	156, 1314
BtgI	CCRYGG	1 cut(s)	1276
BtgZI	GCGATG	1 cut(s)	773
BtrI	CACGTC	1 cut(s)	433
BtsCI	GGATG	2 cut(s)	283, 714
BtsIMutI	CAGTG	1 cut(s)	1023
Cac8I	GCNNGC	4 cut(s)	372, 937, 1141, 1172
CaiI	CAGNNNCTG	2 cut(s)	599, 1022
CfoI	GCGC	3 cut(s)	325, 370, 1125
Cfr10I	RCCGGY	1 cut(s)	325
Cfr13I	GGNCC	2 cut(s)	155, 1313
Csp6I	GTAC	3 cut(s)	1154, 1289, 1378
CviAII	CATG	7 cut(s)	97, 304, 733, 999, 1204, 1277, 1332
CviQI	GTAC	3 cut(s)	1154, 1289, 1378
DdeI	CTNAG	5 cut(s)	459, 585, 657, 1292, 1346
DpnI	GATC	7 cut(s)	252, 295, 383, 713, 1209, 1274, 1344
DpnII	GATC	7 cut(s)	250, 293, 381, 711, 1207, 1272, 1342
Eam1104I	CTCTTC	1 cut(s)	555
EarI	CTCTTC	1 cut(s)	555
Eco130I	CCWWGG	2 cut(s)	158, 1276
Eco31I	GGTCTC	1 cut(s)	1101
Eco32I	GATATC	1 cut(s)	425
Eco57I	CTGAAG	3 cut(s)	171, 342, 1062
EcoNI	CCTNNNNNAGG	3 cut(s)	1007, 1259, 1320
EcoO109I	RGGNCCY	1 cut(s)	155
EcoRII	CCWGG	1 cut(s)	1315
EcoRV	GATATC	1 cut(s)	425
EcoT14I	CCWWGG	2 cut(s)	158, 1276
ErhI	CCWWGG	2 cut(s)	158, 1276
FaeI	CATG	7 cut(s)	100, 307, 736, 1002, 1207, 1280, 1335
FalI	AAGNNNNNCTT	2 cut(s)	924, 956
FaqI	GGGAC	2 cut(s)	86, 995
FatI	CATG	7 cut(s)	96, 303, 732, 998, 1203, 1276, 1331
FauI	CCCGC	2 cut(s)	94, 412
FbaI	TGATCA	1 cut(s)	381
FokI	GGATG	2 cut(s)	290, 721
FspBI	CTAG	5 cut(s)	66, 873, 1082, 1187, 1245
GlaI	GCGC	3 cut(s)	324, 369, 1124
GsaI	CCCAGC	1 cut(s)	44
HaeIII	GGCC	2 cut(s)	156, 1314
HapII	CCGG	6 cut(s)	128, 174, 210, 326, 705, 808
HhaI	GCGC	3 cut(s)	325, 370, 1125
Hin1II	CATG	7 cut(s)	100, 307, 736, 1002, 1207, 1280, 1335
Hin6I	GCGC	3 cut(s)	323, 368, 1123
HinP1I	GCGC	3 cut(s)	323, 368, 1123
HincII	GTYRAC	2 cut(s)	582, 805
HindII	GTYRAC	2 cut(s)	582, 805
HinfI	GANTC	5 cut(s)	82, 169, 198, 497, 681
HpaII	CCGG	6 cut(s)	128, 174, 210, 326, 705, 808
HphI	GGTGA	3 cut(s)	425, 1280, 1319
Hpy166II	GTNNAC	4 cut(s)	300, 582, 805, 1380
Hpy188I	TCNGA	1 cut(s)	361
Hpy188III	TCNNGA	5 cut(s)	66, 79, 428, 885, 1258
Hpy8I	GTNNAC	4 cut(s)	300, 582, 805, 1380
HpyAV	CCTTC	5 cut(s)	146, 188, 983, 1168, 1255
HpyCH4III	ACNGT	5 cut(s)	829, 859, 1018, 1062, 1285
HpyCH4IV	ACGT	1 cut(s)	432
HpyCH4V	TGCA	9 cut(s)	191, 339, 404, 602, 955, 1028, 1094, 1139, 1148
HpyF3I	CTNAG	5 cut(s)	459, 585, 657, 1292, 1346
HpySE526I	ACGT	1 cut(s)	432
Hsp92II	CATG	7 cut(s)	100, 307, 736, 1002, 1207, 1280, 1335
HspAI	GCGC	3 cut(s)	323, 368, 1123
Ksp22I	TGATCA	1 cut(s)	381
Kzo9I	GATC	7 cut(s)	250, 293, 381, 711, 1207, 1272, 1342
LmnI	GCTCC	3 cut(s)	810, 992, 1179
LweI	GCATC	5 cut(s)	268, 406, 586, 745, 1079
MaeI	CTAG	5 cut(s)	66, 873, 1082, 1187, 1245
MaeII	ACGT	1 cut(s)	432
MaeIII	GTNAC	8 cut(s)	119, 181, 262, 745, 1012, 1018, 1247, 1307
MalI	GATC	7 cut(s)	252, 295, 383, 713, 1209, 1274, 1344
MboI	GATC	7 cut(s)	250, 293, 381, 711, 1207, 1272, 1342
MboII	GAAGA	5 cut(s)	218, 542, 891, 1065, 1068
MfeI	CAATTG	1 cut(s)	470
MflI	RGATCY	1 cut(s)	1272
MhlI	GDGCHC	1 cut(s)	446
MluCI	AATT	8 cut(s)	35, 470, 565, 648, 795, 1005, 1223, 1365
MlyI	GAGTC	1 cut(s)	690
MmeI	TCCRAC	1 cut(s)	949
MnlI	CCTC	7 cut(s)	27, 475, 580, 1003, 1030, 1265, 1316
MroXI	GAANNNNTTC	1 cut(s)	537
MseI	TTAA	3 cut(s)	564, 1362, 1368
MslI	CAYNNNNRTG	1 cut(s)	308
MspA1I	CMGCKG	1 cut(s)	320
MspI	CCGG	6 cut(s)	128, 174, 210, 326, 705, 808
MspR9I	CCNGG	3 cut(s)	174, 706, 1317
MunI	CAATTG	1 cut(s)	470
MvaI	CCWGG	1 cut(s)	1317
MvnI	CGCG	1 cut(s)	370
NciI	CCSGG	2 cut(s)	174, 706
NcoI	CCATGG	1 cut(s)	1276
NdeII	GATC	7 cut(s)	250, 293, 381, 711, 1207, 1272, 1342
NlaIII	CATG	7 cut(s)	100, 307, 736, 1002, 1207, 1280, 1335
NlaIV	GGNNCC	3 cut(s)	703, 889, 1218
NmuCI	GTSAC	5 cut(s)	119, 1012, 1018, 1247, 1307
NspI	RCATGY	1 cut(s)	1002
PasI	CCCWGGG	1 cut(s)	1316
PdmI	GAANNNNTTC	1 cut(s)	537
PfeI	GAWTC	4 cut(s)	82, 169, 198, 497
PfoI	TCCNGGA	1 cut(s)	704
PleI	GAGTC	1 cut(s)	689
PpsI	GAGTC	1 cut(s)	689
PshBI	ATTAAT	1 cut(s)	1368
Psp6I	CCWGG	1 cut(s)	1315
PspFI	CCCAGC	1 cut(s)	40
PspGI	CCWGG	1 cut(s)	1315
PspN4I	GGNNCC	3 cut(s)	703, 889, 1218
PspPI	GGNCC	2 cut(s)	155, 1313
PstI	CTGCAG	3 cut(s)	193, 604, 1141
PstNI	CAGNNNCTG	2 cut(s)	599, 1022
PsuI	RGATCY	1 cut(s)	1272
PvuII	CAGCTG	1 cut(s)	320
RsaI	GTAC	3 cut(s)	1155, 1290, 1379
RsaNI	GTAC	3 cut(s)	1154, 1289, 1378
RseI	CAYNNNNRTG	1 cut(s)	308
SaqAI	TTAA	3 cut(s)	564, 1362, 1368
Sau3AI	GATC	7 cut(s)	250, 293, 381, 711, 1207, 1272, 1342
Sau96I	GGNCC	2 cut(s)	155, 1313
SchI	GAGTC	1 cut(s)	690
ScrFI	CCNGG	3 cut(s)	174, 706, 1317
SduI	GDGCHC	1 cut(s)	446
SfaNI	GCATC	5 cut(s)	268, 406, 586, 745, 1079
SfcI	CTRYAG	3 cut(s)	189, 600, 1137
SgrAI	CRCCGGYG	1 cut(s)	325
SmiMI	CAYNNNNRTG	1 cut(s)	308
SmlI	CTYRAG	2 cut(s)	445, 1256
SmoI	CTYRAG	2 cut(s)	445, 1256
SpeI	ACTAGT	1 cut(s)	1081
Sse9I	AATT	8 cut(s)	35, 470, 565, 648, 795, 1005, 1223, 1365
SsiI	CCGC	4 cut(s)	87, 222, 419, 1241
SspMI	CTAG	5 cut(s)	66, 873, 1082, 1187, 1245
StyD4I	CCNGG	3 cut(s)	172, 704, 1315
StyI	CCWWGG	2 cut(s)	158, 1276
TaaI	ACNGT	5 cut(s)	829, 859, 1018, 1062, 1285
TaiI	ACGT	1 cut(s)	435
TaqI	TCGA	3 cut(s)	500, 1161, 1230
TasI	AATT	8 cut(s)	35, 470, 565, 648, 795, 1005, 1223, 1365
TatI	WGTACW	2 cut(s)	1288, 1377
TauI	GCSGC	1 cut(s)	1244
TfiI	GAWTC	4 cut(s)	82, 169, 198, 497
Tru1I	TTAA	3 cut(s)	564, 1362, 1368
Tru9I	TTAA	3 cut(s)	564, 1362, 1368
TscAI	CASTG	1 cut(s)	1023
TseFI	GTSAC	5 cut(s)	119, 1012, 1018, 1247, 1307
Tsp45I	GTSAC	5 cut(s)	119, 1012, 1018, 1247, 1307
TspDTI	ATGAA	2 cut(s)	320, 930
TspGWI	ACGGA	3 cut(s)	19, 999, 1291
TspRI	CASTG	1 cut(s)	1023
VspI	ATTAAT	1 cut(s)	1368
XagI	CCTNNNNNAGG	3 cut(s)	1007, 1259, 1320
XapI	RAATTY	1 cut(s)	35
XbaI	TCTAGA	1 cut(s)	65
XceI	RCATGY	1 cut(s)	1002
XmnI	GAANNNNTTC	1 cut(s)	537
XspI	CTAG	5 cut(s)	66, 873, 1082, 1187, 1245

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.