Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0012708

Physical Location & Seq

Genomic Coordinates Reverse (-)

24261314 .. 24266879

Gene Structure 5566 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1154500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGGGAAATTGCTGCAGATCTCCGGCCGCTGTCGCCAGAGAGGACGTGAAATCGAACTTTTCCGGGCACGATCACGGCCGGAGGGACTCCAACGCCGGGAAGAAGGCTCCGACGACGGTCTTGACTGGCGTGCCGAAGGAGAATATAGAGGAGAAGTATTTGGTGGATCGGGAGCTCGGCCGGGGGGAGTTCGGCGTTACTTACCTCTGTATTGACCGCCATAGTCGTGAGCTCTTGGCCTGTAAGAGCATTTCGAAGCGGAAGCTCAGGACTGCCGTGGACGTCGAGGATGTGCGGCGCGAGGTGGCGATAATGCAGCACTTGCCGAAGAATTCGAGCATTGTGAGCTTGAAGGAGGCTTGCGAGGACGAGAACGCCGTGCATTTGGTGATGGAGTTGTGCGAGGGCGGTGAGCTCTTCGATCGGATTGTGGCGAGGGGGCATTATACGGAGCGAGCTGCGGCGGCCGTAACCAGGACCATTGTTGAGGTTGTACAGCTCTGCCACAAGCATGGAGTGATTCATAGGGACTTGAAGCCAGAGAACTTTTTGTTCGCCAACAAGAAGGAGAATTCGCCTCTCAAGGCGATTGATTTCGGCTTGTCTATATTCTTCAAACCAGGTGAGAGGTTCTCAGAAATTGTTGGAAGCCCATACTACATGGCTCCTGAGGTGCTCAAGAGGAATTACGGGCCAGAAATAGATATATGGAGTGCAGGAGTTATACTGTATATTTTGTTGTGTGGGGTGCCCCCTTTTTGGGCTGAGTCTGAACAAGGGGTTGCACAGGCAATTCTACGTGGGCTGATAGATTTCAAGCGTGATCCATGGCCCAATATTTCGGAGAGTGCCAAGAGTTTAGTTAGGCAAATGTTGGAGCCAGACCCAAAGCTACGATTGACTGCAAAGCAAGTTCTTGAGCATCCTTGGCTCCAAAATGCTAAGAAAGCACCGAACATTCCCCTTGGAGATGTTGTCAAGTCAAGGCTCAAGCAGTTTTCAATGATGAACAGATTCAAAAGAAAGGCCTTGAGGGTTATTGCAGATTTCTTTTCCGTTGATGAAGTTGAAGACAGTAAGGAGATGTTTAAGAAGATAGACACTGATAATGATGGAATTGTTTCAATTGAAGAATTGAAATCTGGACTTCGGAATTTTGGATCCCAGCTTGCCGATTCTGACATTCAGTTGCTTATTGAAGCAGTTGATACTAATGGGAAGGGTACTCTGGACTGTGGAGAATTTATTGCCGTTACCCTCCACCTACAGAGAATGGCAAATGATGAGCATCTTCACAAGGCATTTTCCTACTTTGACAAGAATAACAACGGCTATATTGAACCAGACGAGCTCAGGGATGCATTGATGGAAGATGGGGCGGATGATTGTACAGATGTAGCAAATGACATATTCCAAGAAGTGGACACAGACAAGGATGGGCGTATCAGCTATGAAGAATTTGTCGCCATGATGAAAACCGGGACAGATTGGAGAAAGGCTTCTCGTCATTACTCAAGAGGGAGATTCAACAGTCTAAGCATAAAGCTGATGAAGGATGGTTCCATAAATTTGGGGAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

528

Amino Acids

59.44

Weight (kDa)

5.95

Isoelectric Point (pI)

38.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	54 - 311	2.6e-76	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	56 - 307	2e-24	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	358 - 418	8.3e-10	EF-hand domain pair
EF-hand_1	PF00036	360 - 385	1.5e-06	EF hand domain
EF-hand_6	PF13405	360 - 387	4.9e-06	EF-hand domain
EF-hand_7	PF13499	429 - 492	2.2e-17	EF-hand domain pair
EF-hand_1	PF00036	431 - 456	9.9e-07	EF hand domain
EF-hand_6	PF13405	431 - 457	7.9e-07	EF-hand domain
EF-hand_1	PF00036	468 - 493	6e-09	EF hand domain
EF-hand_5	PF13202	469 - 491	4.9e-07	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0012708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51850 AT3G51850 AT3G51850
fragaria_vesca	FvH4_6g11340
malus_domestica	MD04G1154500.v1.1 MD12G1168200.v1.1
prunus_persica	Prupe.6G278500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0462921
rosa_laevigata	RLG00000024823
rosa_multiflora	Rmu_sc0000766.1_g000018
rosa_roxburghii	Rroxscaffold_6G00417100
rosa_rugosa	Rorug03G0059800 Rorug03G0059900
rosa_samantha	Rh3AG118100 Rh3BG123000 Rh3CG124800 Rh3DG123500
rosa_wichuraiana	Rw3G010020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	285
AccB1I	GGYRCC	1 cut(s)	748
AccB7I	CCANNNNNTGG	1 cut(s)	1420
AccII	CGCG	1 cut(s)	300
AciI	CCGC	8 cut(s)	27, 217, 259, 295, 408, 461, 464, 1379
AclWI	GGATC	4 cut(s)	174, 818, 1155, 1168
AcoI	YGGCCR	4 cut(s)	24, 76, 178, 465
AcsI	RAATTY	6 cut(s)	331, 571, 1153, 1241, 1457, 1567
AcyI	GRCGYC	1 cut(s)	282
AfaI	GTAC	3 cut(s)	495, 1225, 1390
AfiI	CCNNNNNNNGG	5 cut(s)	96, 474, 759, 760, 1420
AjiI	CACGTC	1 cut(s)	46
AjnI	CCWGG	2 cut(s)	473, 619
Alw21I	GWGCWC	5 cut(s)	177, 234, 417, 678, 1353
AlwI	GGATC	4 cut(s)	174, 818, 1155, 1168
AoxI	GGCC	8 cut(s)	24, 76, 178, 237, 465, 692, 830, 1026
ApeKI	GCWGC	3 cut(s)	12, 316, 458
ApoI	RAATTY	6 cut(s)	331, 571, 1153, 1241, 1457, 1567
Asp700I	GAANNNNTTC	3 cut(s)	1013, 1120, 1498
AspLEI	GCGC	1 cut(s)	300
AspS9I	GGNCC	3 cut(s)	477, 692, 831
AsuC2I	CCSGG	4 cut(s)	64, 97, 182, 1480
AsuHPI	GGTGA	3 cut(s)	400, 422, 635
AsuII	TTCGAA	1 cut(s)	254
AvaII	GGWCC	1 cut(s)	477
AxyI	CCTNAGG	1 cut(s)	669
BaeGI	GKGCMC	2 cut(s)	69, 753
BamHI	GGATCC	1 cut(s)	1160
BanI	GGYRCC	1 cut(s)	748
BanII	GRGCYC	4 cut(s)	177, 234, 417, 1353
BbsI	GAAGAC	1 cut(s)	1077
Bbv12I	GWGCWC	5 cut(s)	177, 234, 417, 678, 1353
BbvI	GCAGC	2 cut(s)	328, 445
BccI	CCATC	6 cut(s)	385, 1106, 1360, 1367, 1430, 1550
BceAI	ACGGC	6 cut(s)	91, 260, 362, 452, 1235, 1345
BciT130I	CCWGG	2 cut(s)	475, 621
BcnI	CCSGG	4 cut(s)	64, 97, 182, 1480
BfmI	CTRYAG	2 cut(s)	13, 1265
BglII	AGATCT	1 cut(s)	17
BisI	GCNGC	7 cut(s)	13, 27, 296, 317, 459, 462, 465
BlsI	GCNGC	7 cut(s)	14, 28, 297, 318, 460, 463, 466
Bme1390I	CCNGG	6 cut(s)	64, 97, 182, 475, 621, 1480
Bme18I	GGWCC	1 cut(s)	477
BmgBI	CACGTC	1 cut(s)	46
BmgT120I	GGNCC	3 cut(s)	477, 692, 831
BmiI	GGNNCC	7 cut(s)	108, 666, 750, 879, 932, 1162, 1561
BmrFI	CCNGG	6 cut(s)	64, 97, 182, 475, 621, 1480
BmsI	GCATC	3 cut(s)	931, 1297, 1348
BoxI	GACNNNNGTC	1 cut(s)	116
BpiI	GAAGAC	1 cut(s)	1077
BplI	GAGNNNNNCTC	2 cut(s)	617, 649
Bpu10I	CCTNAGC	2 cut(s)	266, 1352
Bpu14I	TTCGAA	1 cut(s)	254
BpuEI	CTTGAG	6 cut(s)	566, 662, 938, 974, 1051, 1498
BpuMI	CCSGG	4 cut(s)	64, 97, 182, 1480
BsaAI	YACGTR	1 cut(s)	800
BsaBI	GATNNNNATC	1 cut(s)	1287
BsaHI	GRCGYC	1 cut(s)	282
BsaJI	CCNNGG	5 cut(s)	181, 276, 827, 926, 964
BsaXI	ACNNNNNCTCC	2 cut(s)	960, 990
Bsc4I	CCNNNNNNNGG	5 cut(s)	96, 474, 759, 760, 1420
Bse1I	ACTGG	1 cut(s)	130
Bse21I	CCTNAGG	1 cut(s)	669
Bse8I	GATNNNNATC	1 cut(s)	1287
BseBI	CCWGG	2 cut(s)	475, 621
BseDI	CCNNGG	5 cut(s)	181, 276, 827, 926, 964
BseGI	GGATG	6 cut(s)	295, 922, 1363, 1387, 1441, 1561
BseJI	GATNNNNATC	1 cut(s)	1287
BseLI	CCNNNNNNNGG	5 cut(s)	96, 474, 759, 760, 1420
BseMII	CTCAG	5 cut(s)	280, 648, 660, 756, 1366
BseNI	ACTGG	1 cut(s)	130
BseRI	GAGGAG	1 cut(s)	164
BseSI	GKGCMC	2 cut(s)	69, 753
BseX3I	CGGCCG	4 cut(s)	24, 76, 178, 465
BseXI	GCAGC	2 cut(s)	328, 445
BseYI	CCCAGC	1 cut(s)	1164
BsgI	GTGCAG	1 cut(s)	735
Bsh1236I	CGCG	1 cut(s)	300
Bsh1285I	CGRYCG	5 cut(s)	27, 79, 181, 424, 468
BshFI	GGCC	8 cut(s)	26, 78, 180, 239, 467, 694, 832, 1028
BshNI	GGYRCC	1 cut(s)	748
BsiEI	CGRYCG	5 cut(s)	27, 79, 181, 424, 468
BsiHKAI	GWGCWC	5 cut(s)	177, 234, 417, 678, 1353
BsiSI	CCGG	6 cut(s)	23, 63, 79, 96, 181, 1479
BslFI	GGGAC	3 cut(s)	98, 542, 1495
BslI	CCNNNNNNNGG	5 cut(s)	96, 474, 759, 760, 1420
BsmFI	GGGAC	3 cut(s)	98, 542, 1495
BsnI	GGCC	8 cut(s)	26, 78, 180, 239, 467, 694, 832, 1028
Bsp119I	TTCGAA	1 cut(s)	254
Bsp1286I	GDGCHC	7 cut(s)	69, 177, 234, 417, 678, 753, 1353
Bsp1407I	TGTACA	2 cut(s)	493, 1388
Bsp143I	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
Bsp19I	CCATGG	1 cut(s)	827
BspACI	CCGC	8 cut(s)	27, 217, 259, 295, 408, 461, 464, 1379
BspANI	GGCC	8 cut(s)	26, 78, 180, 239, 467, 694, 832, 1028
BspCNI	CTCAG	5 cut(s)	279, 647, 661, 757, 1365
BspFNI	CGCG	1 cut(s)	300
BspLI	GGNNCC	7 cut(s)	108, 666, 750, 879, 932, 1162, 1561
BspMAI	CTGCAG	1 cut(s)	17
BspPI	GGATC	4 cut(s)	174, 818, 1155, 1168
BspQI	GCTCTTC	1 cut(s)	422
BspT104I	TTCGAA	1 cut(s)	254
BspT107I	GGYRCC	1 cut(s)	748
BsrGI	TGTACA	2 cut(s)	493, 1388
BsrI	ACTGG	1 cut(s)	130
BssECI	CCNNGG	5 cut(s)	181, 276, 827, 926, 964
BssMI	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
BssNI	GRCGYC	1 cut(s)	282
BssT1I	CCWWGG	3 cut(s)	827, 926, 964
Bst2UI	CCWGG	2 cut(s)	475, 621
Bst4CI	ACNGT	5 cut(s)	118, 729, 1076, 1235, 1532
Bst6I	CTCTTC	1 cut(s)	422
BstACI	GRCGYC	1 cut(s)	282
BstAPI	GCANNNNNTGC	1 cut(s)	322
BstAUI	TGTACA	2 cut(s)	493, 1388
BstBAI	YACGTR	1 cut(s)	800
BstBI	TTCGAA	1 cut(s)	254
BstC8I	GCNNGC	4 cut(s)	131, 361, 456, 1170
BstDEI	CTNAG	7 cut(s)	266, 634, 669, 765, 942, 1352, 1535
BstDSI	CCRYGG	2 cut(s)	276, 827
BstF5I	GGATG	6 cut(s)	295, 922, 1363, 1387, 1441, 1561
BstFNI	CGCG	1 cut(s)	300
BstHHI	GCGC	1 cut(s)	300
BstKTI	GATC	6 cut(s)	20, 73, 169, 424, 826, 1163
BstMBI	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
BstMCI	CGRYCG	5 cut(s)	27, 79, 181, 424, 468
BstMWI	GCNNNNNNNGC	6 cut(s)	32, 322, 345, 464, 928, 947
BstNI	CCWGG	2 cut(s)	475, 621
BstPAI	GACNNNNGTC	1 cut(s)	116
BstSCI	CCNGG	6 cut(s)	62, 95, 180, 473, 619, 1478
BstSFI	CTRYAG	2 cut(s)	13, 1265
BstSLI	GKGCMC	2 cut(s)	69, 753
BstUI	CGCG	1 cut(s)	300
BstV1I	GCAGC	2 cut(s)	328, 445
BstV2I	GAAGAC	1 cut(s)	1077
BstX2I	RGATCY	2 cut(s)	17, 1160
BstXI	CCANNNNNNTGG	2 cut(s)	512, 1570
BstYI	RGATCY	2 cut(s)	17, 1160
BstZI	CGGCCG	4 cut(s)	24, 76, 178, 465
Bsu36I	CCTNAGG	1 cut(s)	669
BsuRI	GGCC	8 cut(s)	26, 78, 180, 239, 467, 694, 832, 1028
BtgI	CCRYGG	2 cut(s)	276, 827
BtrI	CACGTC	1 cut(s)	46
BtsCI	GGATG	6 cut(s)	295, 922, 1363, 1387, 1441, 1561
BtsIMutI	CAGTG	1 cut(s)	1101
Cac8I	GCNNGC	4 cut(s)	131, 361, 456, 1170
CfoI	GCGC	1 cut(s)	300
Cfr13I	GGNCC	3 cut(s)	477, 692, 831
CsiI	ACCWGGT	1 cut(s)	619
Csp6I	GTAC	3 cut(s)	494, 1224, 1389
CviAII	CATG	4 cut(s)	512, 661, 828, 1468
CviQI	GTAC	3 cut(s)	494, 1224, 1389
DdeI	CTNAG	7 cut(s)	266, 634, 669, 765, 942, 1352, 1535
DpnI	GATC	6 cut(s)	19, 72, 168, 423, 825, 1162
DpnII	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
EaeI	YGGCCR	4 cut(s)	24, 76, 178, 465
EagI	CGGCCG	4 cut(s)	24, 76, 178, 465
Eam1104I	CTCTTC	1 cut(s)	422
EarI	CTCTTC	1 cut(s)	422
EciI	GGCGGA	1 cut(s)	1394
Ecl136II	GAGCTC	4 cut(s)	175, 232, 415, 1351
EclXI	CGGCCG	4 cut(s)	24, 76, 178, 465
Eco130I	CCWWGG	3 cut(s)	827, 926, 964
Eco147I	AGGCCT	1 cut(s)	1028
Eco24I	GRGCYC	4 cut(s)	177, 234, 417, 1353
Eco47I	GGWCC	1 cut(s)	477
Eco52I	CGGCCG	4 cut(s)	24, 76, 178, 465
Eco53kI	GAGCTC	4 cut(s)	175, 232, 415, 1351
Eco81I	CCTNAGG	1 cut(s)	669
EcoICRI	GAGCTC	4 cut(s)	175, 232, 415, 1351
EcoRI	GAATTC	2 cut(s)	331, 571
EcoRII	CCWGG	2 cut(s)	473, 619
EcoT14I	CCWWGG	3 cut(s)	827, 926, 964
EcoT22I	ATGCAT	1 cut(s)	1363
EcoT38I	GRGCYC	4 cut(s)	177, 234, 417, 1353
ErhI	CCWWGG	3 cut(s)	827, 926, 964
FaeI	CATG	4 cut(s)	515, 664, 831, 1471
FaqI	GGGAC	3 cut(s)	98, 542, 1495
FatI	CATG	4 cut(s)	511, 660, 827, 1467
Fnu4HI	GCNGC	7 cut(s)	13, 27, 296, 317, 459, 462, 465
FokI	GGATG	6 cut(s)	302, 909, 1370, 1394, 1448, 1568
FriOI	GRGCYC	4 cut(s)	177, 234, 417, 1353
Fsp4HI	GCNGC	7 cut(s)	13, 27, 296, 317, 459, 462, 465
GlaI	GCGC	1 cut(s)	299
GluI	GCNGC	7 cut(s)	13, 27, 296, 317, 459, 462, 465
GsaI	CCCAGC	1 cut(s)	1168
HaeIII	GGCC	8 cut(s)	26, 78, 180, 239, 467, 694, 832, 1028
HapII	CCGG	6 cut(s)	23, 63, 79, 96, 181, 1479
HhaI	GCGC	1 cut(s)	300
Hin1I	GRCGYC	1 cut(s)	282
Hin1II	CATG	4 cut(s)	515, 664, 831, 1471
Hin6I	GCGC	1 cut(s)	298
HinP1I	GCGC	1 cut(s)	298
HinfI	GANTC	6 cut(s)	86, 520, 767, 1014, 1175, 1524
HpaII	CCGG	6 cut(s)	23, 63, 79, 96, 181, 1479
HphI	GGTGA	3 cut(s)	400, 422, 635
Hpy166II	GTNNAC	2 cut(s)	280, 1423
Hpy188I	TCNGA	7 cut(s)	111, 426, 637, 772, 844, 1152, 1180
Hpy8I	GTNNAC	2 cut(s)	280, 1423
Hpy99I	CGWCG	3 cut(s)	115, 118, 287
HpyAV	CCTTC	6 cut(s)	97, 130, 346, 559, 1213, 1546
HpyCH4III	ACNGT	5 cut(s)	118, 729, 1076, 1235, 1532
HpyCH4IV	ACGT	3 cut(s)	45, 282, 799
HpyCH4V	TGCA	8 cut(s)	15, 316, 382, 716, 785, 905, 1043, 1361
HpyF10VI	GCNNNNNNNGC	6 cut(s)	32, 322, 345, 464, 928, 947
HpyF3I	CTNAG	7 cut(s)	266, 634, 669, 765, 942, 1352, 1535
HpySE526I	ACGT	3 cut(s)	45, 282, 799
Hsp92I	GRCGYC	1 cut(s)	282
Hsp92II	CATG	4 cut(s)	515, 664, 831, 1471
HspAI	GCGC	1 cut(s)	298
Kzo9I	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
LguI	GCTCTTC	1 cut(s)	422
LmnI	GCTCC	6 cut(s)	112, 172, 451, 670, 877, 936
Lsp1109I	GCAGC	2 cut(s)	328, 445
LweI	GCATC	3 cut(s)	931, 1297, 1348
MabI	ACCWGGT	1 cut(s)	619
MaeII	ACGT	3 cut(s)	45, 282, 799
MaeIII	GTNAC	3 cut(s)	196, 469, 1252
MalI	GATC	6 cut(s)	19, 72, 168, 423, 825, 1162
MboI	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
MfeI	CAATTG	1 cut(s)	1125
MflI	RGATCY	2 cut(s)	17, 1160
MhlI	GDGCHC	7 cut(s)	69, 177, 234, 417, 678, 753, 1353
MlyI	GAGTC	2 cut(s)	80, 776
MmeI	TCCRAC	4 cut(s)	114, 134, 625, 855
Mph1103I	ATGCAT	1 cut(s)	1363
MroXI	GAANNNNTTC	3 cut(s)	1013, 1120, 1498
MseI	TTAA	1 cut(s)	1089
MslI	CAYNNNNRTG	2 cut(s)	225, 510
MspA1I	CMGCKG	1 cut(s)	29
MspI	CCGG	6 cut(s)	23, 63, 79, 96, 181, 1479
MspR9I	CCNGG	6 cut(s)	64, 97, 182, 475, 621, 1480
MunI	CAATTG	1 cut(s)	1125
MvaI	CCWGG	2 cut(s)	475, 621
MvnI	CGCG	1 cut(s)	300
MwoI	GCNNNNNNNGC	6 cut(s)	32, 322, 345, 464, 928, 947
NciI	CCSGG	4 cut(s)	64, 97, 182, 1480
NcoI	CCATGG	1 cut(s)	827
NdeII	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
NlaIII	CATG	4 cut(s)	515, 664, 831, 1471
NlaIV	GGNNCC	7 cut(s)	108, 666, 750, 879, 932, 1162, 1561
NmeAIII	GCCGAG	1 cut(s)	156
NsiI	ATGCAT	1 cut(s)	1363
NspV	TTCGAA	1 cut(s)	254
PceI	AGGCCT	1 cut(s)	1028
PciSI	GCTCTTC	1 cut(s)	422
PcsI	WCGNNNNNNNCGW	1 cut(s)	375
PdmI	GAANNNNTTC	3 cut(s)	1013, 1120, 1498
PfeI	GAWTC	4 cut(s)	520, 1014, 1175, 1524
PflMI	CCANNNNNTGG	1 cut(s)	1420
PkrI	GCNGC	7 cut(s)	14, 28, 297, 318, 460, 463, 466
Ple19I	CGATCG	1 cut(s)	424
PleI	GAGTC	2 cut(s)	80, 775
PpsI	GAGTC	2 cut(s)	80, 775
Ppu21I	YACGTR	1 cut(s)	800
PshAI	GACNNNNGTC	1 cut(s)	116
Psp124BI	GAGCTC	4 cut(s)	177, 234, 417, 1353
Psp6I	CCWGG	2 cut(s)	473, 619
PspFI	CCCAGC	1 cut(s)	1164
PspGI	CCWGG	2 cut(s)	473, 619
PspN4I	GGNNCC	7 cut(s)	108, 666, 750, 879, 932, 1162, 1561
PspPI	GGNCC	3 cut(s)	477, 692, 831
PstI	CTGCAG	1 cut(s)	17
PsuI	RGATCY	2 cut(s)	17, 1160
PvuI	CGATCG	1 cut(s)	424
RsaI	GTAC	3 cut(s)	495, 1225, 1390
RsaNI	GTAC	3 cut(s)	494, 1224, 1389
RseI	CAYNNNNRTG	2 cut(s)	225, 510
SacI	GAGCTC	4 cut(s)	177, 234, 417, 1353
SapI	GCTCTTC	1 cut(s)	422
SaqAI	TTAA	1 cut(s)	1089
SatI	GCNGC	7 cut(s)	13, 27, 296, 317, 459, 462, 465
Sau3AI	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
Sau96I	GGNCC	3 cut(s)	477, 692, 831
SchI	GAGTC	2 cut(s)	80, 776
ScrFI	CCNGG	6 cut(s)	64, 97, 182, 475, 621, 1480
SduI	GDGCHC	7 cut(s)	69, 177, 234, 417, 678, 753, 1353
SexAI	ACCWGGT	1 cut(s)	619
SfaNI	GCATC	3 cut(s)	931, 1297, 1348
SfcI	CTRYAG	2 cut(s)	13, 1265
SfuI	TTCGAA	1 cut(s)	254
SinI	GGWCC	1 cut(s)	477
SmiMI	CAYNNNNRTG	2 cut(s)	225, 510
SmlI	CTYRAG	6 cut(s)	581, 677, 917, 989, 1030, 1513
SmoI	CTYRAG	6 cut(s)	581, 677, 917, 989, 1030, 1513
SseBI	AGGCCT	1 cut(s)	1028
SsiI	CCGC	8 cut(s)	27, 217, 259, 295, 408, 461, 464, 1379
SspI	AATATT	1 cut(s)	838
SstI	GAGCTC	4 cut(s)	177, 234, 417, 1353
StuI	AGGCCT	1 cut(s)	1028
StyD4I	CCNGG	6 cut(s)	62, 95, 180, 473, 619, 1478
StyI	CCWWGG	3 cut(s)	827, 926, 964
TaaI	ACNGT	5 cut(s)	118, 729, 1076, 1235, 1532
TaiI	ACGT	3 cut(s)	48, 285, 802
TaqI	TCGA	5 cut(s)	53, 254, 285, 335, 420
TatI	WGTACW	2 cut(s)	493, 1388
TauI	GCSGC	4 cut(s)	29, 298, 464, 467
TfiI	GAWTC	4 cut(s)	520, 1014, 1175, 1524
Tru1I	TTAA	1 cut(s)	1089
Tru9I	TTAA	1 cut(s)	1089
TscAI	CASTG	1 cut(s)	1108
TseI	GCWGC	3 cut(s)	12, 316, 458
TspDTI	ATGAA	6 cut(s)	512, 1022, 1077, 1467, 1487, 1565
TspGWI	ACGGA	2 cut(s)	464, 1045
TspRI	CASTG	1 cut(s)	1108
Van91I	CCANNNNNTGG	1 cut(s)	1420
VpaK11BI	GGWCC	1 cut(s)	477
XapI	RAATTY	6 cut(s)	331, 571, 1153, 1241, 1457, 1567
XmnI	GAANNNNTTC	3 cut(s)	1013, 1120, 1498
ZraI	GACGTC	1 cut(s)	283
Zsp2I	ATGCAT	1 cut(s)	1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.