Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012708

Physical Location & Seq

Genomic Coordinates Reverse (-)

24847173 .. 24852786

Gene Structure 5614 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1168200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGGGAAATTGCTGCAGATCTCCGGCCGCCGTCGCTAGAGAGGACGTGAAATCAAACTTTTCCGGGCACGATCAAGGAAGGAGGGACTCCAGCGCCGGGAAGAAGGCACCGACGACGGTGTTGACCGGCGTGCCGAAGGAGAATGTAGAGGAGAAGTACCTGGTGGATCGGGAGCTTGGCCGGGGGGAGTTCGGCGTCACGTACCTCTGTATTGACCGCCATAGTCGTGAGCTTTTGGCTTGCAAGAGCATTTCGAAGCGGAAGCTCAGGACCGCTGTGGACGTCGAGGATGTGCGGCGCGAAGTGGCGATAATGAAGCACTTGCCGAAGAATTCGAGCATTGTGAGTTTGAAGGAGGCTTGCGAGGACGAGAACGCCGTGCATTTGGTGATGGAGTTGTGCGAAGGCGGTGAGCTCTTCGATCGGATTGTGGCGAGGGGGCATTATACGGAGCGGGCCGCGGCGGCGGTAACCAGGACGATTGTTGAGGTTGTGCAGCTCTGCCACAAGCATGGAGTGATTCATAGGGACTTGAAGCCGGAGAATTTCCTGTTCGCAAACAAGAAGGAGAATTCGCCTCTCAAGGCCATTGATTTCGGCTTGTCTATATTCTTCAAGCCAGGTGAGAGGTTCTCAGAAATTGTTGGAAGTCCATACTACATGGCTCCTGAGGTGCTCAAGAGGAATTACGGGCCAGAAATAGATATATGGAGTGCAGGAGTTATACTGTATATTTTGTTGTGTGGGGTGCCCCCCTTTTGGGCTGAGTCTGAACAAGGGGTTGCACAGGCAATTCTACGTGGGCTGATAGATTTCAAGCGTGATCCATGGCCCAATATTTCGGAGAGTGCCAAGGGTTTAGTTAGGCAAATGTTGGAGCCAGACCCAAAGCTACGATTGACTGCGAAGCAAGTTCTTGAGCATCCTTGGCTCCAAAATGCTAAGAAAGCACCGAACATTCCCCTTGGAGATGTTGTCAAGTCAAGGCTTAAGCAGTTTTCAATGATGAACAGATTCAAAAGAAAAGCCTTGAGGGTTATTGCAGATTTCTTTTCCGTTGATGAAGTTGAAGACAGTAAGGAGATGTTTAAGAAGATAGACACTGATAATGATGGAATTGTTTCAATTGAAGAATTAAAATCTGGACTTCGGAATTTTGGATCCCAGCTTGCCGATTCTGACATTCAATTGCTTATTGAAGCAGTTGATACTAATGGGAAGGGTACGCTGGACTGTGGAGAATTTATTGCCGTGACCCTCCACCTACAGAGAATGGCAAATGATGAGCATCTTCACAAGGCATTTTCCTACTTTGACAAGAACAGCAACGGCTATATTGAGCCAGACGAGCTCCGGGATGCATTGATGGAAGATGGGGCAGATGATTGTACAGATGTAGCAAATGACATATTTCAAGAAGTGGACACAGACAAGGACGGGCGTATCAGCTATGAAGAATTTGTCGCCATGATGAAAACCGGGACAGATTGGAGAAAGGCTTCTCGGCATTACTCAAGAGGGAGATTCAACAGTCTAAGCATAAAGCTGATGAAGGATGGTTCCATAAATTTGGGGAATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

528

Amino Acids

59.33

Weight (kDa)

5.99

Isoelectric Point (pI)

37.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	54 - 311	6.9e-76	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	56 - 307	3e-24	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	358 - 418	8.3e-10	EF-hand domain pair
EF-hand_1	PF00036	360 - 385	1.5e-06	EF hand domain
EF-hand_6	PF13405	360 - 387	4.9e-06	EF-hand domain
EF-hand_7	PF13499	429 - 492	1.1e-16	EF-hand domain pair
EF-hand_1	PF00036	431 - 456	6.5e-06	EF hand domain
EF-hand_6	PF13405	431 - 457	4e-06	EF-hand domain
EF-hand_1	PF00036	468 - 493	6e-09	EF hand domain
EF-hand_5	PF13202	469 - 491	4.9e-07	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0012708)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51850 AT3G51850 AT3G51850
fragaria_vesca	FvH4_6g11340
malus_domestica	MD04G1154500.v1.1 MD12G1168200.v1.1
prunus_persica	Prupe.6G278500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0462921
rosa_laevigata	RLG00000024823
rosa_multiflora	Rmu_sc0000766.1_g000018
rosa_roxburghii	Rroxscaffold_6G00417100
rosa_rugosa	Rorug03G0059800 Rorug03G0059900
rosa_samantha	Rh3AG118100 Rh3BG123000 Rh3CG124800 Rh3DG123500
rosa_wichuraiana	Rw3G010020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	285
AccB1I	GGYRCC	2 cut(s)	106, 748
AccBSI	CCGCTC	1 cut(s)	454
AccII	CGCG	2 cut(s)	300, 461
AclWI	GGATC	4 cut(s)	174, 818, 1155, 1168
AcoI	YGGCCR	2 cut(s)	24, 178
AcsI	RAATTY	7 cut(s)	331, 544, 571, 1153, 1241, 1457, 1567
AcyI	GRCGYC	2 cut(s)	195, 282
AfaI	GTAC	4 cut(s)	158, 203, 1225, 1390
AfiI	CCNNNNNNNGG	3 cut(s)	96, 759, 760
AflII	CTTAAG	1 cut(s)	989
AjiI	CACGTC	1 cut(s)	46
AjnI	CCWGG	3 cut(s)	159, 473, 619
Alw21I	GWGCWC	3 cut(s)	417, 678, 1353
AlwI	GGATC	4 cut(s)	174, 818, 1155, 1168
AoxI	GGCC	6 cut(s)	24, 178, 456, 585, 692, 830
ApeKI	GCWGC	2 cut(s)	12, 496
ApoI	RAATTY	7 cut(s)	331, 544, 571, 1153, 1241, 1457, 1567
Asp700I	GAANNNNTTC	3 cut(s)	1013, 1120, 1498
AspLEI	GCGC	2 cut(s)	95, 300
AspS9I	GGNCC	4 cut(s)	270, 456, 692, 831
AsuC2I	CCSGG	5 cut(s)	64, 97, 182, 1355, 1480
AsuHPI	GGTGA	3 cut(s)	400, 422, 635
AsuII	TTCGAA	1 cut(s)	254
AvaII	GGWCC	1 cut(s)	270
AxyI	CCTNAGG	1 cut(s)	669
BaeGI	GKGCMC	2 cut(s)	69, 753
BaeI	ACNNNNGTAYC	2 cut(s)	1426, 1459
BamHI	GGATCC	1 cut(s)	1160
BanI	GGYRCC	2 cut(s)	106, 748
BanII	GRGCYC	2 cut(s)	417, 1353
BbsI	GAAGAC	1 cut(s)	1077
Bbv12I	GWGCWC	3 cut(s)	417, 678, 1353
BbvI	GCAGC	1 cut(s)	508
BccI	CCATC	5 cut(s)	385, 1106, 1360, 1367, 1550
BceAI	ACGGC	4 cut(s)	14, 362, 1235, 1345
BciT130I	CCWGG	3 cut(s)	161, 475, 621
BcnI	CCSGG	5 cut(s)	64, 97, 182, 1355, 1480
BfaI	CTAG	1 cut(s)	36
BfmI	CTRYAG	2 cut(s)	13, 1265
BfoI	RGCGCY	1 cut(s)	96
BfrI	CTTAAG	1 cut(s)	989
BglI	GCCNNNNNGGC	1 cut(s)	464
BglII	AGATCT	1 cut(s)	17
BisI	GCNGC	7 cut(s)	13, 27, 296, 459, 462, 465, 497
BlsI	GCNGC	7 cut(s)	14, 28, 297, 460, 463, 466, 498
Bme1390I	CCNGG	8 cut(s)	64, 97, 161, 182, 475, 621, 1355, 1480
Bme18I	GGWCC	1 cut(s)	270
BmgBI	CACGTC	1 cut(s)	46
BmgT120I	GGNCC	4 cut(s)	270, 456, 692, 831
BmiI	GGNNCC	7 cut(s)	108, 666, 750, 879, 932, 1162, 1561
BmrFI	CCNGG	8 cut(s)	64, 97, 161, 182, 475, 621, 1355, 1480
BmsI	GCATC	3 cut(s)	931, 1297, 1348
BpiI	GAAGAC	1 cut(s)	1077
BplI	GAGNNNNNCTC	2 cut(s)	617, 649
BpmI	CTGGAG	1 cut(s)	73
Bpu10I	CCTNAGC	1 cut(s)	266
Bpu14I	TTCGAA	1 cut(s)	254
BpuEI	CTTGAG	5 cut(s)	566, 662, 938, 1051, 1498
BpuMI	CCSGG	5 cut(s)	64, 97, 182, 1355, 1480
BsaAI	YACGTR	2 cut(s)	201, 800
BsaBI	GATNNNNATC	1 cut(s)	1287
BsaHI	GRCGYC	2 cut(s)	195, 282
BsaJI	CCNNGG	6 cut(s)	181, 459, 827, 852, 926, 964
BsaXI	ACNNNNNCTCC	2 cut(s)	960, 990
Bsc4I	CCNNNNNNNGG	3 cut(s)	96, 759, 760
Bse118I	RCCGGY	1 cut(s)	125
Bse21I	CCTNAGG	1 cut(s)	669
Bse8I	GATNNNNATC	1 cut(s)	1287
BseBI	CCWGG	3 cut(s)	161, 475, 621
BseDI	CCNNGG	6 cut(s)	181, 459, 827, 852, 926, 964
BseGI	GGATG	4 cut(s)	295, 922, 1363, 1561
BseJI	GATNNNNATC	1 cut(s)	1287
BseLI	CCNNNNNNNGG	3 cut(s)	96, 759, 760
BseMII	CTCAG	4 cut(s)	280, 648, 660, 756
BseRI	GAGGAG	1 cut(s)	164
BseSI	GKGCMC	2 cut(s)	69, 753
BseX3I	CGGCCG	1 cut(s)	24
BseXI	GCAGC	1 cut(s)	508
BseYI	CCCAGC	1 cut(s)	1164
BsgI	GTGCAG	2 cut(s)	515, 735
Bsh1236I	CGCG	2 cut(s)	300, 461
Bsh1285I	CGRYCG	2 cut(s)	27, 424
BshFI	GGCC	6 cut(s)	26, 180, 458, 587, 694, 832
BshNI	GGYRCC	2 cut(s)	106, 748
BsiEI	CGRYCG	2 cut(s)	27, 424
BsiHKAI	GWGCWC	3 cut(s)	417, 678, 1353
BsiSI	CCGG	8 cut(s)	23, 63, 96, 126, 181, 539, 1354, 1479
BslFI	GGGAC	3 cut(s)	98, 542, 1495
BslI	CCNNNNNNNGG	3 cut(s)	96, 759, 760
BsmFI	GGGAC	3 cut(s)	98, 542, 1495
BsnI	GGCC	6 cut(s)	26, 180, 458, 587, 694, 832
Bsp119I	TTCGAA	1 cut(s)	254
Bsp1286I	GDGCHC	5 cut(s)	69, 417, 678, 753, 1353
Bsp1407I	TGTACA	1 cut(s)	1388
Bsp143I	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
Bsp19I	CCATGG	1 cut(s)	827
BspANI	GGCC	6 cut(s)	26, 180, 458, 587, 694, 832
BspCNI	CTCAG	4 cut(s)	279, 647, 661, 757
BspFNI	CGCG	2 cut(s)	300, 461
BspLI	GGNNCC	7 cut(s)	108, 666, 750, 879, 932, 1162, 1561
BspMAI	CTGCAG	1 cut(s)	17
BspPI	GGATC	4 cut(s)	174, 818, 1155, 1168
BspQI	GCTCTTC	1 cut(s)	422
BspT104I	TTCGAA	1 cut(s)	254
BspT107I	GGYRCC	2 cut(s)	106, 748
BspTI	CTTAAG	1 cut(s)	989
BsrBI	CCGCTC	1 cut(s)	454
BsrFI	RCCGGY	1 cut(s)	125
BsrGI	TGTACA	1 cut(s)	1388
BssAI	RCCGGY	1 cut(s)	125
BssECI	CCNNGG	6 cut(s)	181, 459, 827, 852, 926, 964
BssMI	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
BssNI	GRCGYC	2 cut(s)	195, 282
BssT1I	CCWWGG	4 cut(s)	827, 852, 926, 964
Bst2UI	CCWGG	3 cut(s)	161, 475, 621
Bst4CI	ACNGT	5 cut(s)	118, 729, 1076, 1235, 1532
Bst6I	CTCTTC	1 cut(s)	422
BstACI	GRCGYC	2 cut(s)	195, 282
BstAFI	CTTAAG	1 cut(s)	989
BstAUI	TGTACA	1 cut(s)	1388
BstBAI	YACGTR	2 cut(s)	201, 800
BstBI	TTCGAA	1 cut(s)	254
BstC8I	GCNNGC	5 cut(s)	131, 241, 361, 456, 1170
BstDEI	CTNAG	6 cut(s)	266, 634, 669, 765, 942, 1535
BstDSI	CCRYGG	2 cut(s)	459, 827
BstEII	GGTNACC	1 cut(s)	469
BstF5I	GGATG	4 cut(s)	295, 922, 1363, 1561
BstFNI	CGCG	2 cut(s)	300, 461
BstH2I	RGCGCY	1 cut(s)	96
BstHHI	GCGC	2 cut(s)	95, 300
BstKTI	GATC	6 cut(s)	20, 73, 169, 424, 826, 1163
BstMBI	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
BstMCI	CGRYCG	2 cut(s)	27, 424
BstMWI	GCNNNNNNNGC	5 cut(s)	32, 464, 928, 947, 1348
BstNI	CCWGG	3 cut(s)	161, 475, 621
BstPI	GGTNACC	1 cut(s)	469
BstSCI	CCNGG	8 cut(s)	62, 95, 159, 180, 473, 619, 1353, 1478
BstSFI	CTRYAG	2 cut(s)	13, 1265
BstSLI	GKGCMC	2 cut(s)	69, 753
BstUI	CGCG	2 cut(s)	300, 461
BstV1I	GCAGC	1 cut(s)	508
BstV2I	GAAGAC	1 cut(s)	1077
BstX2I	RGATCY	2 cut(s)	17, 1160
BstXI	CCANNNNNNTGG	2 cut(s)	512, 1570
BstYI	RGATCY	2 cut(s)	17, 1160
BstZI	CGGCCG	1 cut(s)	24
Bsu36I	CCTNAGG	1 cut(s)	669
BsuRI	GGCC	6 cut(s)	26, 180, 458, 587, 694, 832
BtgI	CCRYGG	2 cut(s)	459, 827
BtrI	CACGTC	1 cut(s)	46
BtsCI	GGATG	4 cut(s)	295, 922, 1363, 1561
BtsIMutI	CAGTG	1 cut(s)	1101
Cac8I	GCNNGC	5 cut(s)	131, 241, 361, 456, 1170
CfoI	GCGC	2 cut(s)	95, 300
Cfr10I	RCCGGY	1 cut(s)	125
Cfr13I	GGNCC	4 cut(s)	270, 456, 692, 831
Cfr42I	CCGCGG	1 cut(s)	462
CseI	GACGC	1 cut(s)	184
CsiI	ACCWGGT	1 cut(s)	159
Csp6I	GTAC	4 cut(s)	157, 202, 1224, 1389
CviAII	CATG	4 cut(s)	512, 661, 828, 1468
CviQI	GTAC	4 cut(s)	157, 202, 1224, 1389
DdeI	CTNAG	6 cut(s)	266, 634, 669, 765, 942, 1535
DpnI	GATC	6 cut(s)	19, 72, 168, 423, 825, 1162
DpnII	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
EaeI	YGGCCR	2 cut(s)	24, 178
EagI	CGGCCG	1 cut(s)	24
Eam1104I	CTCTTC	1 cut(s)	422
EarI	CTCTTC	1 cut(s)	422
Ecl136II	GAGCTC	2 cut(s)	415, 1351
EclXI	CGGCCG	1 cut(s)	24
Eco130I	CCWWGG	4 cut(s)	827, 852, 926, 964
Eco24I	GRGCYC	2 cut(s)	417, 1353
Eco47I	GGWCC	1 cut(s)	270
Eco52I	CGGCCG	1 cut(s)	24
Eco53kI	GAGCTC	2 cut(s)	415, 1351
Eco81I	CCTNAGG	1 cut(s)	669
Eco91I	GGTNACC	1 cut(s)	469
EcoICRI	GAGCTC	2 cut(s)	415, 1351
EcoO65I	GGTNACC	1 cut(s)	469
EcoRI	GAATTC	2 cut(s)	331, 571
EcoRII	CCWGG	3 cut(s)	159, 473, 619
EcoT14I	CCWWGG	4 cut(s)	827, 852, 926, 964
EcoT22I	ATGCAT	1 cut(s)	1363
EcoT38I	GRGCYC	2 cut(s)	417, 1353
ErhI	CCWWGG	4 cut(s)	827, 852, 926, 964
FaeI	CATG	4 cut(s)	515, 664, 831, 1471
FaqI	GGGAC	3 cut(s)	98, 542, 1495
FatI	CATG	4 cut(s)	511, 660, 827, 1467
FauI	CCCGC	1 cut(s)	447
Fnu4HI	GCNGC	7 cut(s)	13, 27, 296, 459, 462, 465, 497
FokI	GGATG	4 cut(s)	302, 909, 1370, 1568
FriOI	GRGCYC	2 cut(s)	417, 1353
Fsp4HI	GCNGC	7 cut(s)	13, 27, 296, 459, 462, 465, 497
FspBI	CTAG	1 cut(s)	36
GlaI	GCGC	2 cut(s)	94, 299
GluI	GCNGC	7 cut(s)	13, 27, 296, 459, 462, 465, 497
GsaI	CCCAGC	1 cut(s)	1168
GsuI	CTGGAG	1 cut(s)	73
HaeII	RGCGCY	1 cut(s)	96
HaeIII	GGCC	6 cut(s)	26, 180, 458, 587, 694, 832
HapII	CCGG	8 cut(s)	23, 63, 96, 126, 181, 539, 1354, 1479
HgaI	GACGC	1 cut(s)	184
HhaI	GCGC	2 cut(s)	95, 300
Hin1I	GRCGYC	2 cut(s)	195, 282
Hin1II	CATG	4 cut(s)	515, 664, 831, 1471
Hin6I	GCGC	2 cut(s)	93, 298
HinP1I	GCGC	2 cut(s)	93, 298
HincII	GTYRAC	1 cut(s)	123
HindII	GTYRAC	1 cut(s)	123
HinfI	GANTC	6 cut(s)	86, 520, 767, 1014, 1175, 1524
HpaII	CCGG	8 cut(s)	23, 63, 96, 126, 181, 539, 1354, 1479
HphI	GGTGA	3 cut(s)	400, 422, 635
Hpy166II	GTNNAC	3 cut(s)	123, 280, 1423
Hpy188I	TCNGA	6 cut(s)	426, 637, 772, 844, 1152, 1180
Hpy188III	TCNNGA	9 cut(s)	170, 227, 268, 668, 679, 917, 1143, 1415, 1515
Hpy8I	GTNNAC	3 cut(s)	123, 280, 1423
Hpy99I	CGWCG	4 cut(s)	35, 115, 118, 287
HpyAV	CCTTC	8 cut(s)	72, 97, 130, 346, 398, 559, 1213, 1546
HpyCH4III	ACNGT	5 cut(s)	118, 729, 1076, 1235, 1532
HpyCH4IV	ACGT	4 cut(s)	45, 200, 282, 799
HpyCH4V	TGCA	8 cut(s)	15, 243, 382, 496, 716, 785, 1043, 1361
HpyF10VI	GCNNNNNNNGC	5 cut(s)	32, 464, 928, 947, 1348
HpyF3I	CTNAG	6 cut(s)	266, 634, 669, 765, 942, 1535
HpySE526I	ACGT	4 cut(s)	45, 200, 282, 799
Hsp92I	GRCGYC	2 cut(s)	195, 282
Hsp92II	CATG	4 cut(s)	515, 664, 831, 1471
HspAI	GCGC	2 cut(s)	93, 298
KspI	CCGCGG	1 cut(s)	462
Kzo9I	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
LguI	GCTCTTC	1 cut(s)	422
LmnI	GCTCC	6 cut(s)	172, 451, 670, 877, 936, 1356
Lsp1109I	GCAGC	1 cut(s)	508
LweI	GCATC	3 cut(s)	931, 1297, 1348
MabI	ACCWGGT	1 cut(s)	159
MaeI	CTAG	1 cut(s)	36
MaeII	ACGT	4 cut(s)	45, 200, 282, 799
MaeIII	GTNAC	3 cut(s)	196, 469, 1252
MalI	GATC	6 cut(s)	19, 72, 168, 423, 825, 1162
MbiI	CCGCTC	1 cut(s)	454
MboI	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
MfeI	CAATTG	2 cut(s)	1125, 1187
MflI	RGATCY	2 cut(s)	17, 1160
MhlI	GDGCHC	5 cut(s)	69, 417, 678, 753, 1353
MlyI	GAGTC	2 cut(s)	80, 776
MmeI	TCCRAC	2 cut(s)	625, 855
Mph1103I	ATGCAT	1 cut(s)	1363
MroXI	GAANNNNTTC	3 cut(s)	1013, 1120, 1498
MseI	TTAA	3 cut(s)	990, 1089, 1136
MslI	CAYNNNNRTG	2 cut(s)	225, 510
MspA1I	CMGCKG	2 cut(s)	275, 461
MspCI	CTTAAG	1 cut(s)	989
MspI	CCGG	8 cut(s)	23, 63, 96, 126, 181, 539, 1354, 1479
MspR9I	CCNGG	8 cut(s)	64, 97, 161, 182, 475, 621, 1355, 1480
MunI	CAATTG	2 cut(s)	1125, 1187
MvaI	CCWGG	3 cut(s)	161, 475, 621
MvnI	CGCG	2 cut(s)	300, 461
MwoI	GCNNNNNNNGC	5 cut(s)	32, 464, 928, 947, 1348
NciI	CCSGG	5 cut(s)	64, 97, 182, 1355, 1480
NcoI	CCATGG	1 cut(s)	827
NdeII	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
NlaIII	CATG	4 cut(s)	515, 664, 831, 1471
NlaIV	GGNNCC	7 cut(s)	108, 666, 750, 879, 932, 1162, 1561
NmeAIII	GCCGAG	1 cut(s)	1483
NmuCI	GTSAC	2 cut(s)	196, 1252
NsiI	ATGCAT	1 cut(s)	1363
NspV	TTCGAA	1 cut(s)	254
PciSI	GCTCTTC	1 cut(s)	422
PcsI	WCGNNNNNNNCGW	1 cut(s)	375
PdmI	GAANNNNTTC	3 cut(s)	1013, 1120, 1498
PfeI	GAWTC	4 cut(s)	520, 1014, 1175, 1524
PfoI	TCCNGGA	1 cut(s)	1353
PkrI	GCNGC	7 cut(s)	14, 28, 297, 460, 463, 466, 498
Ple19I	CGATCG	1 cut(s)	424
PleI	GAGTC	2 cut(s)	80, 775
PpsI	GAGTC	2 cut(s)	80, 775
Ppu21I	YACGTR	2 cut(s)	201, 800
Psp124BI	GAGCTC	2 cut(s)	417, 1353
Psp6I	CCWGG	3 cut(s)	159, 473, 619
PspEI	GGTNACC	1 cut(s)	469
PspFI	CCCAGC	1 cut(s)	1164
PspGI	CCWGG	3 cut(s)	159, 473, 619
PspN4I	GGNNCC	7 cut(s)	108, 666, 750, 879, 932, 1162, 1561
PspPI	GGNCC	4 cut(s)	270, 456, 692, 831
PstI	CTGCAG	1 cut(s)	17
PsuI	RGATCY	2 cut(s)	17, 1160
PvuI	CGATCG	1 cut(s)	424
RsaI	GTAC	4 cut(s)	158, 203, 1225, 1390
RsaNI	GTAC	4 cut(s)	157, 202, 1224, 1389
RseI	CAYNNNNRTG	2 cut(s)	225, 510
SacI	GAGCTC	2 cut(s)	417, 1353
SacII	CCGCGG	1 cut(s)	462
SapI	GCTCTTC	1 cut(s)	422
SaqAI	TTAA	3 cut(s)	990, 1089, 1136
SatI	GCNGC	7 cut(s)	13, 27, 296, 459, 462, 465, 497
Sau3AI	GATC	6 cut(s)	17, 70, 166, 421, 823, 1160
Sau96I	GGNCC	4 cut(s)	270, 456, 692, 831
SchI	GAGTC	2 cut(s)	80, 776
ScrFI	CCNGG	8 cut(s)	64, 97, 161, 182, 475, 621, 1355, 1480
SduI	GDGCHC	5 cut(s)	69, 417, 678, 753, 1353
SexAI	ACCWGGT	1 cut(s)	159
SfaNI	GCATC	3 cut(s)	931, 1297, 1348
SfcI	CTRYAG	2 cut(s)	13, 1265
Sfr303I	CCGCGG	1 cut(s)	462
SfuI	TTCGAA	1 cut(s)	254
SgrBI	CCGCGG	1 cut(s)	462
SinI	GGWCC	1 cut(s)	270
SmiMI	CAYNNNNRTG	2 cut(s)	225, 510
SmlI	CTYRAG	6 cut(s)	581, 677, 917, 989, 1030, 1513
SmoI	CTYRAG	6 cut(s)	581, 677, 917, 989, 1030, 1513
SspI	AATATT	1 cut(s)	838
SspMI	CTAG	1 cut(s)	36
SstI	GAGCTC	2 cut(s)	417, 1353
StyD4I	CCNGG	8 cut(s)	62, 95, 159, 180, 473, 619, 1353, 1478
StyI	CCWWGG	4 cut(s)	827, 852, 926, 964
TaaI	ACNGT	5 cut(s)	118, 729, 1076, 1235, 1532
TaiI	ACGT	4 cut(s)	48, 203, 285, 802
TaqI	TCGA	4 cut(s)	254, 285, 335, 420
TatI	WGTACW	1 cut(s)	1388
TauI	GCSGC	5 cut(s)	29, 298, 461, 464, 467
TfiI	GAWTC	4 cut(s)	520, 1014, 1175, 1524
Tru1I	TTAA	3 cut(s)	990, 1089, 1136
Tru9I	TTAA	3 cut(s)	990, 1089, 1136
TscAI	CASTG	1 cut(s)	1108
TseFI	GTSAC	2 cut(s)	196, 1252
TseI	GCWGC	2 cut(s)	12, 496
Tsp45I	GTSAC	2 cut(s)	196, 1252
TspDTI	ATGAA	7 cut(s)	329, 512, 1022, 1077, 1467, 1487, 1565
TspGWI	ACGGA	2 cut(s)	464, 1045
TspRI	CASTG	1 cut(s)	1108
Vha464I	CTTAAG	1 cut(s)	989
VpaK11BI	GGWCC	1 cut(s)	270
XapI	RAATTY	7 cut(s)	331, 544, 571, 1153, 1241, 1457, 1567
XmnI	GAANNNNTTC	3 cut(s)	1013, 1120, 1498
XspI	CTAG	1 cut(s)	36
ZraI	GACGTC	1 cut(s)	283
Zsp2I	ATGCAT	1 cut(s)	1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.