Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009355

Physical Location & Seq

Genomic Coordinates Forward (+)

26589785 .. 26593319

Gene Structure 3535 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1174400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1653 bp

ATGGGGTGCTTCACAGGTTTGAAGAGCAAGAAGAAGAAGTCTGAGCAGACTATTCATATTAAACGTGTCCCGAAGGAGCAAAAACCAACTACGTTGCCTGAACCCAAGACCCGGTCATTACAGTCTGCACCCCCGAGTTTTAGGAACAGAGTGAAACCTATTCAACCAGCTAATAGAGTCGTCAGCAATAGAGCACGGGCATTATCAGCGCCATCGACCCTTGATGCGGCAGACCAAGACGATCTCTCTGAATATGAAGAACCAGAAGACCCAAAGTACCGGTCTGGAACAGTGAAGGAACAATTGTCACCGGTTCCCCAACCTCTTCCTCTCCCGTCACCCCAGGTTGCTGCTGCACTGAGGACTACAGGAAGTTTTAAGTCGGTGACTAATAGCGGCTCTCTCTATGCTTCTGGACCATTGCCACTGCCTCCTACAGCAGCACTACCAACTGGAACACTACCTGCCGGAACACTACGGAACTTTTTGTATGAAGAAATAGCATCAGCTTGTCACAACTTTTCTTCAGACCAATGCTTGTCGGAAGGCCTTTCATCTATTATATATAAGGCTTCCTTTGGAGATGATTCATCGAGCTCAAAGAAGTTTGAAGCTACTGTTACTCGCCTTCACCCGTCCACTCAGGGTTTAAAGGAGTTCATAAATGAGATAAACACCCTTGCGACTTTGCAACATCCAAACCTTTGTAAATTGCTTGGCTTCCATGCACGTGAGGGCTCGGAACAGAAAATGTTGGTGTATGAGAGGCTCAATCATGGAAGTTTAGACCGGCTTTTGTATGGAAGATCAGACAAGCCACCAATAGACTGGAATACAAGAATGAAAATTGCTTTATGTGCTGCACAAGGTCTTACTTTCTTGCATGAAGAAGGGCCTTTCCAGGCGATGTACTATGAATTTTCAACCGCCAACGTACAGATTGATAAAGATTTTAGTGCAAAGCTTTCAGGATATGGTTGTGTTGGCCAAATTCCGGAGGCAGAGATCTCTACTAATTCCGTTGCTGTGGCAAATCTATCAGTGGAGACACTCGAGAGAGGAATGCTCACTCCAAAAAGCAACGTCTGGAGTTTCGGGATTCTTCTTCTGGAACTACTCACTGGCCGGAGGAATTTAGACAGCCGTCATTCTAAGGAAGAAAGAAATTTAGTTAAGTGGAGCCGGCCTTTCTTAGCCGATGATTGCCGACTTTCACTAATCATGGATCCTCAGCTGAAAGGCCGCTTCCCATCCCAAGCAGCCAGAAGGGTGGCAGACATTGCACAGAAATGCCTTCAGAAGGACCCATCAGAAAGACCTACCATGAGAACCGTAGTTGAGCATCTCAAGATCATACAAGACATGAAGCACTCGTGCAGGTTTCCATTGCAAGAGCCAGCATTTGCCGGAAAACACATGTCAAGATCACCCAGCCTTAACGGAATTCTTACCCCTGCACCTCGGTTAAGTTTCTCTCCTTCACCACCATCAGCCGCCCGTCCATCCATTTCACCTACCAGACGACCTGCCCTACTAATGTCTCTTCCTCCGCGTGCTTGCTCCTCCACCCTCACCTTTGAGGAGCTCGACCGGCAGGGAAGCCGGAAATCATCATCATCGACTCTTCCTAGGGCTAGTGTTGAGGGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

551

Amino Acids

60.58

Weight (kDa)

9.41

Isoelectric Point (pI)

59.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	178 - 449	7e-27	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	214 - 448	8.6e-26	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009355)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08760 AT3G08760 AT3G08760
fragaria_vesca	FvH4_6g09920
malus_domestica	MD04G1174400.v1.1 MD12G1188000.v1.1
prunus_persica	Prupe.6G293300_v2.0.a1
pyrus_communis	pycom04g15390 pycom12g17570
rosa_chinensis	RchiOBHm_Chr3g0460511
rosa_laevigata	RLG00000024998 RLG00000025004
rosa_multiflora	Rmu_sc0004267.1_g000004 Rmu_sc0006046.1_g000002 Rmu_sc0030082.1_g000001
rosa_roxburghii	Rroxscaffold_6G00419420
rosa_rugosa	Rorug03G0043300
rosa_samantha	Rh3AG101300 Rh3BG105300 Rh3CG107100 Rh3DG106600
rosa_wichuraiana	Rw3G008620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	472, 1368, 1534
AccII	CGCG	1 cut(s)	1552
AccIII	TCCGGA	1 cut(s)	994
AciI	CCGC	6 cut(s)	227, 396, 927, 1243, 1494, 1550
AclWI	GGATC	2 cut(s)	1220, 1233
AcoI	YGGCCR	2 cut(s)	985, 1123
AcsI	RAATTY	5 cut(s)	917, 990, 1132, 1165, 1443
AcuI	CTGAAG	2 cut(s)	510, 1280
AcvI	CACGTG	1 cut(s)	731
AfaI	GTAC	3 cut(s)	278, 911, 936
AfiI	CCNNNNNNNGG	4 cut(s)	111, 226, 994, 1300
AflIII	ACRYGT	2 cut(s)	64, 1416
AgeI	ACCGGT	2 cut(s)	279, 310
AgsI	TTSAA	4 cut(s)	22, 164, 611, 924
AjnI	CCWGG	2 cut(s)	342, 900
AluBI	AGCT	7 cut(s)	170, 509, 597, 614, 964, 1234, 1585
AluI	AGCT	7 cut(s)	170, 509, 597, 614, 964, 1234, 1585
Alw21I	GWGCWC	3 cut(s)	196, 599, 1587
Alw26I	GTCTC	2 cut(s)	1040, 1545
AlwI	GGATC	2 cut(s)	1220, 1233
Ama87I	CYCGRG	2 cut(s)	133, 1052
Aor13HI	TCCGGA	1 cut(s)	994
AoxI	GGCC	6 cut(s)	547, 893, 985, 1123, 1184, 1240
ApeKI	GCWGC	5 cut(s)	350, 353, 440, 860, 1259
ApoI	RAATTY	5 cut(s)	917, 990, 1132, 1165, 1443
ArsI	GACNNNNNNTTYG	2 cut(s)	266, 298
AsiGI	ACCGGT	2 cut(s)	279, 310
AspA2I	CCTAGG	1 cut(s)	1628
AspLEI	GCGC	1 cut(s)	211
AspS9I	GGNCC	3 cut(s)	416, 893, 1303
AsuC2I	CCSGG	1 cut(s)	112
AsuHPI	GGTGA	8 cut(s)	300, 330, 397, 623, 1419, 1473, 1503, 1564
AvaI	CYCGRG	2 cut(s)	133, 1052
AvaII	GGWCC	2 cut(s)	416, 1303
AvrII	CCTAGG	1 cut(s)	1628
BalI	TGGCCA	1 cut(s)	987
BamHI	GGATCC	1 cut(s)	1225
BanII	GRGCYC	3 cut(s)	599, 740, 1587
BauI	CACGAG	1 cut(s)	1372
BbrPI	CACGTG	1 cut(s)	731
BbsI	GAAGAC	1 cut(s)	273
Bbv12I	GWGCWC	3 cut(s)	196, 599, 1587
BbvCI	CCTCAGC	1 cut(s)	1230
BbvI	GCAGC	5 cut(s)	337, 340, 452, 847, 1271
BccI	CCATC	5 cut(s)	220, 1258, 1315, 1495, 1510
BceAI	ACGGC	1 cut(s)	1128
BciT130I	CCWGG	2 cut(s)	344, 902
BcnI	CCSGG	1 cut(s)	112
BcoDI	GTCTC	2 cut(s)	1040, 1545
BfaI	CTAG	2 cut(s)	1629, 1635
BfmI	CTRYAG	2 cut(s)	366, 435
BfoI	RGCGCY	1 cut(s)	212
BfuAI	ACCTGC	3 cut(s)	472, 1368, 1534
BglII	AGATCT	1 cut(s)	1005
BisI	GCNGC	9 cut(s)	228, 351, 354, 397, 441, 861, 1243, 1260, 1494
BlnI	CCTAGG	1 cut(s)	1628
BlsI	GCNGC	9 cut(s)	229, 352, 355, 398, 442, 862, 1244, 1261, 1495
Bme1390I	CCNGG	3 cut(s)	112, 344, 902
Bme18I	GGWCC	2 cut(s)	416, 1303
BmeT110I	CYCGRG	2 cut(s)	133, 1052
BmgT120I	GGNCC	3 cut(s)	416, 893, 1303
BmiI	GGNNCC	4 cut(s)	315, 1181, 1227, 1305
BmrFI	CCNGG	3 cut(s)	112, 344, 902
BmsI	GCATC	3 cut(s)	214, 512, 1351
BoxI	GACNNNNGTC	1 cut(s)	1143
BpiI	GAAGAC	1 cut(s)	273
BplI	GAGNNNNNCTC	2 cut(s)	1050, 1082
BpmI	CTGGAG	1 cut(s)	1108
Bpu10I	CCTNAGC	1 cut(s)	1230
BpuEI	CTTGAG	1 cut(s)	1331
BpuMI	CCSGG	1 cut(s)	112
BsaAI	YACGTR	1 cut(s)	731
BsaJI	CCNNGG	3 cut(s)	342, 1460, 1628
BsaWI	WCCGGW	3 cut(s)	279, 310, 994
Bsc4I	CCNNNNNNNGG	4 cut(s)	111, 226, 994, 1300
Bse118I	RCCGGY	5 cut(s)	279, 310, 789, 1182, 1590
Bse1I	ACTGG	3 cut(s)	457, 833, 1126
Bse3DI	GCAATG	3 cut(s)	419, 1278, 1385
BseAI	TCCGGA	1 cut(s)	994
BseBI	CCWGG	2 cut(s)	344, 902
BseDI	CCNNGG	3 cut(s)	342, 1460, 1628
BseGI	GGATG	3 cut(s)	694, 1250, 1502
BseLI	CCNNNNNNNGG	4 cut(s)	111, 226, 994, 1300
BseMI	GCAATG	3 cut(s)	419, 1278, 1385
BseMII	CTCAG	4 cut(s)	33, 350, 656, 1244
BseNI	ACTGG	3 cut(s)	457, 833, 1126
BseRI	GAGGAG	2 cut(s)	1552, 1595
BseXI	GCAGC	5 cut(s)	337, 340, 452, 847, 1271
BseYI	CCCAGC	1 cut(s)	1430
BsgI	GTGCAG	5 cut(s)	111, 339, 846, 1396, 1440
Bsh1236I	CGCG	1 cut(s)	1552
Bsh1285I	CGRYCG	1 cut(s)	1591
BshFI	GGCC	6 cut(s)	549, 895, 987, 1125, 1186, 1242
BshTI	ACCGGT	2 cut(s)	279, 310
BsiEI	CGRYCG	1 cut(s)	1591
BsiHKAI	GWGCWC	3 cut(s)	196, 599, 1587
BsiHKCI	CYCGRG	2 cut(s)	133, 1052
BslFI	GGGAC	1 cut(s)	53
BslI	CCNNNNNNNGG	4 cut(s)	111, 226, 994, 1300
BsmAI	GTCTC	2 cut(s)	1040, 1545
BsmFI	GGGAC	1 cut(s)	53
BsmI	GAATGC	1 cut(s)	1068
BsnI	GGCC	6 cut(s)	549, 895, 987, 1125, 1186, 1242
BsoBI	CYCGRG	2 cut(s)	133, 1052
Bsp1286I	GDGCHC	4 cut(s)	196, 599, 740, 1587
Bsp13I	TCCGGA	1 cut(s)	994
Bsp143I	GATC	6 cut(s)	241, 806, 1005, 1225, 1350, 1424
BspACI	CCGC	6 cut(s)	227, 396, 927, 1243, 1494, 1550
BspANI	GGCC	6 cut(s)	549, 895, 987, 1125, 1186, 1242
BspCNI	CTCAG	4 cut(s)	34, 351, 655, 1243
BspEI	TCCGGA	1 cut(s)	994
BspFNI	CGCG	1 cut(s)	1552
BspLI	GGNNCC	4 cut(s)	315, 1181, 1227, 1305
BspMI	ACCTGC	3 cut(s)	472, 1368, 1534
BspPI	GGATC	2 cut(s)	1220, 1233
BspQI	GCTCTTC	1 cut(s)	17
BsrDI	GCAATG	3 cut(s)	419, 1278, 1385
BsrFI	RCCGGY	5 cut(s)	279, 310, 789, 1182, 1590
BsrI	ACTGG	3 cut(s)	457, 833, 1126
BssAI	RCCGGY	5 cut(s)	279, 310, 789, 1182, 1590
BssECI	CCNNGG	3 cut(s)	342, 1460, 1628
BssMI	GATC	6 cut(s)	241, 806, 1005, 1225, 1350, 1424
BssSI	CACGAG	1 cut(s)	1372
BssT1I	CCWWGG	1 cut(s)	1628
Bst2BI	CACGAG	1 cut(s)	1372
Bst2UI	CCWGG	2 cut(s)	344, 902
Bst4CI	ACNGT	4 cut(s)	123, 292, 619, 1333
Bst6I	CTCTTC	4 cut(s)	17, 330, 1548, 1629
BstAPI	GCANNNNNTGC	1 cut(s)	1280
BstBAI	YACGTR	1 cut(s)	731
BstC8I	GCNNGC	4 cut(s)	1184, 1398, 1554, 1558
BstDEI	CTNAG	6 cut(s)	42, 359, 642, 1152, 1192, 1230
BstENI	CCTNNNNNAGG	1 cut(s)	1298
BstF5I	GGATG	3 cut(s)	694, 1250, 1502
BstFNI	CGCG	1 cut(s)	1552
BstH2I	RGCGCY	1 cut(s)	212
BstHHI	GCGC	1 cut(s)	211
BstKTI	GATC	6 cut(s)	244, 809, 1008, 1228, 1353, 1427
BstMAI	GTCTC	2 cut(s)	1040, 1545
BstMBI	GATC	6 cut(s)	241, 806, 1005, 1225, 1350, 1424
BstMCI	CGRYCG	1 cut(s)	1591
BstMWI	GCNNNNNNNGC	4 cut(s)	206, 857, 1280, 1591
BstNI	CCWGG	2 cut(s)	344, 902
BstNSI	RCATGY	1 cut(s)	1420
BstPAI	GACNNNNGTC	1 cut(s)	1143
BstSCI	CCNGG	3 cut(s)	110, 342, 900
BstSFI	CTRYAG	2 cut(s)	366, 435
BstUI	CGCG	1 cut(s)	1552
BstV1I	GCAGC	5 cut(s)	337, 340, 452, 847, 1271
BstV2I	GAAGAC	1 cut(s)	273
BstX2I	RGATCY	2 cut(s)	1005, 1225
BstXI	CCANNNNNNTGG	2 cut(s)	828, 1270
BstYI	RGATCY	2 cut(s)	1005, 1225
BsuRI	GGCC	6 cut(s)	549, 895, 987, 1125, 1186, 1242
BtgZI	GCGATG	1 cut(s)	920
BtsCI	GGATG	3 cut(s)	694, 1250, 1502
BtsI	GCAGTG	1 cut(s)	425
BtsIMutI	CAGTG	5 cut(s)	297, 356, 425, 1047, 1119
BveI	ACCTGC	3 cut(s)	472, 1368, 1534
Cac8I	GCNNGC	4 cut(s)	1184, 1398, 1554, 1558
CfoI	GCGC	1 cut(s)	211
Cfr10I	RCCGGY	5 cut(s)	279, 310, 789, 1182, 1590
Cfr13I	GGNCC	3 cut(s)	416, 893, 1303
Csp6I	GTAC	3 cut(s)	277, 910, 935
CspAI	ACCGGT	2 cut(s)	279, 310
CviAII	CATG	7 cut(s)	725, 776, 884, 1222, 1324, 1363, 1417
CviQI	GTAC	3 cut(s)	277, 910, 935
DdeI	CTNAG	6 cut(s)	42, 359, 642, 1152, 1192, 1230
DpnI	GATC	6 cut(s)	243, 808, 1007, 1227, 1352, 1426
DpnII	GATC	6 cut(s)	241, 806, 1005, 1225, 1350, 1424
DraI	TTTAAA	1 cut(s)	651
EaeI	YGGCCR	2 cut(s)	985, 1123
Eam1104I	CTCTTC	4 cut(s)	17, 330, 1548, 1629
EarI	CTCTTC	4 cut(s)	17, 330, 1548, 1629
Ecl136II	GAGCTC	2 cut(s)	597, 1585
Eco130I	CCWWGG	1 cut(s)	1628
Eco147I	AGGCCT	1 cut(s)	549
Eco24I	GRGCYC	3 cut(s)	599, 740, 1587
Eco47I	GGWCC	2 cut(s)	416, 1303
Eco53kI	GAGCTC	2 cut(s)	597, 1585
Eco57I	CTGAAG	2 cut(s)	510, 1280
Eco72I	CACGTG	1 cut(s)	731
Eco88I	CYCGRG	2 cut(s)	133, 1052
EcoICRI	GAGCTC	2 cut(s)	597, 1585
EcoNI	CCTNNNNNAGG	1 cut(s)	1298
EcoO109I	RGGNCCY	2 cut(s)	893, 1303
EcoRI	GAATTC	1 cut(s)	1443
EcoRII	CCWGG	2 cut(s)	342, 900
EcoT14I	CCWWGG	1 cut(s)	1628
EcoT38I	GRGCYC	3 cut(s)	599, 740, 1587
ErhI	CCWWGG	1 cut(s)	1628
FaeI	CATG	7 cut(s)	728, 779, 887, 1225, 1327, 1366, 1420
FalI	AAGNNNNNCTT	2 cut(s)	560, 592
FaqI	GGGAC	1 cut(s)	53
FatI	CATG	7 cut(s)	724, 775, 883, 1221, 1323, 1362, 1416
Fnu4HI	GCNGC	9 cut(s)	228, 351, 354, 397, 441, 861, 1243, 1260, 1494
FokI	GGATG	3 cut(s)	681, 1237, 1489
FriOI	GRGCYC	3 cut(s)	599, 740, 1587
Fsp4HI	GCNGC	9 cut(s)	228, 351, 354, 397, 441, 861, 1243, 1260, 1494
FspBI	CTAG	2 cut(s)	1629, 1635
GlaI	GCGC	1 cut(s)	210
GluI	GCNGC	9 cut(s)	228, 351, 354, 397, 441, 861, 1243, 1260, 1494
GsaI	CCCAGC	1 cut(s)	1434
GsuI	CTGGAG	1 cut(s)	1108
HaeII	RGCGCY	1 cut(s)	212
HaeIII	GGCC	6 cut(s)	549, 895, 987, 1125, 1186, 1242
HhaI	GCGC	1 cut(s)	211
Hin1II	CATG	7 cut(s)	728, 779, 887, 1225, 1327, 1366, 1420
Hin6I	GCGC	1 cut(s)	209
HinP1I	GCGC	1 cut(s)	209
HindIII	AAGCTT	1 cut(s)	962
HinfI	GANTC	4 cut(s)	177, 587, 1099, 1621
HphI	GGTGA	8 cut(s)	300, 330, 397, 623, 1419, 1473, 1503, 1564
Hpy166II	GTNNAC	1 cut(s)	639
Hpy188I	TCNGA	8 cut(s)	43, 250, 529, 544, 742, 811, 1299, 1312
Hpy8I	GTNNAC	1 cut(s)	639
HpyAV	CCTTC	9 cut(s)	67, 289, 539, 638, 884, 1260, 1294, 1304, 1488
HpyCH4III	ACNGT	4 cut(s)	123, 292, 619, 1333
HpyCH4IV	ACGT	5 cut(s)	64, 92, 730, 933, 1083
HpyF10VI	GCNNNNNNNGC	4 cut(s)	206, 857, 1280, 1591
HpyF3I	CTNAG	6 cut(s)	42, 359, 642, 1152, 1192, 1230
HpySE526I	ACGT	5 cut(s)	64, 92, 730, 933, 1083
Hsp92II	CATG	7 cut(s)	728, 779, 887, 1225, 1327, 1366, 1420
HspAI	GCGC	1 cut(s)	209
Kpn2I	TCCGGA	1 cut(s)	994
KroI	GCCGGC	1 cut(s)	1182
KroNI	GCCGGC	1 cut(s)	1184
Kzo9I	GATC	6 cut(s)	241, 806, 1005, 1225, 1350, 1424
LguI	GCTCTTC	1 cut(s)	17
LmnI	GCTCC	4 cut(s)	76, 1179, 1565, 1582
Lsp1109I	GCAGC	5 cut(s)	337, 340, 452, 847, 1271
LweI	GCATC	3 cut(s)	214, 512, 1351
MaeI	CTAG	2 cut(s)	1629, 1635
MaeII	ACGT	5 cut(s)	64, 92, 730, 933, 1083
MaeIII	GTNAC	5 cut(s)	306, 336, 385, 512, 619
MalI	GATC	6 cut(s)	243, 808, 1007, 1227, 1352, 1426
MboI	GATC	6 cut(s)	241, 806, 1005, 1225, 1350, 1424
MfeI	CAATTG	1 cut(s)	302
MflI	RGATCY	2 cut(s)	1005, 1225
MhlI	GDGCHC	4 cut(s)	196, 599, 740, 1587
MlsI	TGGCCA	1 cut(s)	987
MluCI	AATT	9 cut(s)	302, 710, 846, 917, 990, 1015, 1132, 1165, 1443
MluNI	TGGCCA	1 cut(s)	987
MlyI	GAGTC	2 cut(s)	186, 1615
MmeI	TCCRAC	1 cut(s)	522
Mox20I	TGGCCA	1 cut(s)	987
MroI	TCCGGA	1 cut(s)	994
MroNI	GCCGGC	1 cut(s)	1182
MscI	TGGCCA	1 cut(s)	987
MseI	TTAA	6 cut(s)	60, 378, 650, 1173, 1437, 1466
MslI	CAYNNNNRTG	2 cut(s)	729, 1288
Msp20I	TGGCCA	1 cut(s)	987
MspA1I	CMGCKG	1 cut(s)	1234
MspR9I	CCNGG	3 cut(s)	112, 344, 902
MunI	CAATTG	1 cut(s)	302
Mva1269I	GAATGC	1 cut(s)	1068
MvaI	CCWGG	2 cut(s)	344, 902
MvnI	CGCG	1 cut(s)	1552
MwoI	GCNNNNNNNGC	4 cut(s)	206, 857, 1280, 1591
NaeI	GCCGGC	1 cut(s)	1184
NciI	CCSGG	1 cut(s)	112
NdeII	GATC	6 cut(s)	241, 806, 1005, 1225, 1350, 1424
NgoMIV	GCCGGC	1 cut(s)	1182
NlaIII	CATG	7 cut(s)	728, 779, 887, 1225, 1327, 1366, 1420
NlaIV	GGNNCC	4 cut(s)	315, 1181, 1227, 1305
NmuCI	GTSAC	4 cut(s)	306, 336, 385, 512
NspI	RCATGY	1 cut(s)	1420
PaeR7I	CTCGAG	1 cut(s)	1052
PceI	AGGCCT	1 cut(s)	549
PciI	ACATGT	1 cut(s)	1416
PciSI	GCTCTTC	1 cut(s)	17
PctI	GAATGC	1 cut(s)	1068
PdiI	GCCGGC	1 cut(s)	1184
PfeI	GAWTC	2 cut(s)	587, 1099
PflFI	GACNNNGTC	1 cut(s)	112
PinAI	ACCGGT	2 cut(s)	279, 310
PkrI	GCNGC	9 cut(s)	229, 352, 355, 398, 442, 862, 1244, 1261, 1495
PleI	GAGTC	2 cut(s)	185, 1615
PmaCI	CACGTG	1 cut(s)	731
PmlI	CACGTG	1 cut(s)	731
PpsI	GAGTC	2 cut(s)	185, 1615
Ppu21I	YACGTR	1 cut(s)	731
PpuMI	RGGWCCY	1 cut(s)	1303
PscI	ACATGT	1 cut(s)	1416
PshAI	GACNNNNGTC	1 cut(s)	1143
Psp124BI	GAGCTC	2 cut(s)	599, 1587
Psp5II	RGGWCCY	1 cut(s)	1303
Psp6I	CCWGG	2 cut(s)	342, 900
PspCI	CACGTG	1 cut(s)	731
PspFI	CCCAGC	1 cut(s)	1430
PspGI	CCWGG	2 cut(s)	342, 900
PspN4I	GGNNCC	4 cut(s)	315, 1181, 1227, 1305
PspPI	GGNCC	3 cut(s)	416, 893, 1303
PspPPI	RGGWCCY	1 cut(s)	1303
PsuI	RGATCY	2 cut(s)	1005, 1225
PsyI	GACNNNGTC	1 cut(s)	112
PvuII	CAGCTG	1 cut(s)	1234
RsaI	GTAC	3 cut(s)	278, 911, 936
RsaNI	GTAC	3 cut(s)	277, 910, 935
RseI	CAYNNNNRTG	2 cut(s)	729, 1288
SacI	GAGCTC	2 cut(s)	599, 1587
SapI	GCTCTTC	1 cut(s)	17
SaqAI	TTAA	6 cut(s)	60, 378, 650, 1173, 1437, 1466
SatI	GCNGC	9 cut(s)	228, 351, 354, 397, 441, 861, 1243, 1260, 1494
Sau3AI	GATC	6 cut(s)	241, 806, 1005, 1225, 1350, 1424
Sau96I	GGNCC	3 cut(s)	416, 893, 1303
SchI	GAGTC	2 cut(s)	186, 1615
ScrFI	CCNGG	3 cut(s)	112, 344, 902
SduI	GDGCHC	4 cut(s)	196, 599, 740, 1587
SfaNI	GCATC	3 cut(s)	214, 512, 1351
SfcI	CTRYAG	2 cut(s)	366, 435
Sfr274I	CTCGAG	1 cut(s)	1052
SinI	GGWCC	2 cut(s)	416, 1303
SlaI	CTCGAG	1 cut(s)	1052
SmiMI	CAYNNNNRTG	2 cut(s)	729, 1288
SmlI	CTYRAG	2 cut(s)	1052, 1346
SmoI	CTYRAG	2 cut(s)	1052, 1346
Sse9I	AATT	9 cut(s)	302, 710, 846, 917, 990, 1015, 1132, 1165, 1443
SseBI	AGGCCT	1 cut(s)	549
SsiI	CCGC	6 cut(s)	227, 396, 927, 1243, 1494, 1550
SspMI	CTAG	2 cut(s)	1629, 1635
SstI	GAGCTC	2 cut(s)	599, 1587
StuI	AGGCCT	1 cut(s)	549
StyD4I	CCNGG	3 cut(s)	110, 342, 900
StyI	CCWWGG	1 cut(s)	1628
TaaI	ACNGT	4 cut(s)	123, 292, 619, 1333
TaiI	ACGT	5 cut(s)	67, 95, 733, 936, 1086
TaqI	TCGA	5 cut(s)	215, 593, 1053, 1587, 1619
TasI	AATT	9 cut(s)	302, 710, 846, 917, 990, 1015, 1132, 1165, 1443
TatI	WGTACW	1 cut(s)	909
TauI	GCSGC	4 cut(s)	230, 399, 1245, 1496
TfiI	GAWTC	2 cut(s)	587, 1099
Tru1I	TTAA	6 cut(s)	60, 378, 650, 1173, 1437, 1466
Tru9I	TTAA	6 cut(s)	60, 378, 650, 1173, 1437, 1466
TscAI	CASTG	5 cut(s)	297, 363, 432, 1047, 1126
TseFI	GTSAC	4 cut(s)	306, 336, 385, 512
TseI	GCWGC	5 cut(s)	350, 353, 440, 860, 1259
Tsp45I	GTSAC	4 cut(s)	306, 336, 385, 512
TspGWI	ACGGA	3 cut(s)	493, 1009, 1455
TspRI	CASTG	5 cut(s)	297, 363, 432, 1047, 1126
Tth111I	GACNNNGTC	1 cut(s)	112
VpaK11BI	GGWCC	2 cut(s)	416, 1303
XagI	CCTNNNNNAGG	1 cut(s)	1298
XapI	RAATTY	5 cut(s)	917, 990, 1132, 1165, 1443
XceI	RCATGY	1 cut(s)	1420
XcmI	CCANNNNNNNNNTGG	1 cut(s)	825
XhoI	CTCGAG	1 cut(s)	1052
XmaJI	CCTAGG	1 cut(s)	1628
XspI	CTAG	2 cut(s)	1629, 1635

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.