pycom12g17570

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009355
Physical Location & Seq
Forward (+)
19971276 .. 19973608
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g17570.3

Sequence Viewer

Length: 1446 bp
ATGGGGTGCTTCACAGGTTTGAAGTGCAAAAAGAAGAAGTCTGAGCAGACTATTTATAATAAGCGTGTCCCGAAGGAGCAAAAACCAGCTGCATTGCCTGAACCCCAAATCCAGACCCGATCGCTACAGTCTGCACCCCCGAGTTTTAGGAACAGAGTGAAACCTATTCAACCAGTTAATAGAGTCATCAGCAATAGAGCACGAGCATTATCAGCTCCATCGACCCTTGATGCAGCAGACCAAGACGATCTCTCTGAATATGAAGAACCAGAAGACTCAAAGTACCGGCCTGGAATAGTGAAAGAACAATTGTCACCGGTTCCCCAACCTCTTCCTCTCCCATCGCCCCAGGTTGCCGCTGCACTGAGAACTACGGGAAGTTTTAAGTCAGTGACTAATAGTGGCCCTCTCTATGCTTCTGGACCATTGCCACTCCCTCCTACGGCAGCACTACCTACTGGAACAATACCTACTGGAACACTACGGAACTTTTTATATGAAGAAATAGCATCAGCTTGCCACAACTTTTCTTCAGACCGATGCATGTCGGAAGGCCTTTCTTCTATTATATATAAGGCTTCCTTTGGAGATGATTCATTGAGTTCAAAAAAGTTTGAAGCTACTGTTACTCGCCTTCACCCATCCACTCAGGGTTTAAGGGAGTTCATAAATGAGATAAACACCCTTGCAACTTTGCAACATCCAAACCTCTGTAAATTGCTTGGCTTCCATGCACGTGAGGGTTCGGAACAGAAAATGCTGGTGTATGAGAGGCTCTATCATGGAAGTTTAGACCGGCTTTTGTATGGAAGATCAGAGAAGCCTCCGATAGACTGGAATACAAGAATGAAAATTGCTTTATGCGCTGCACAAGGTCTTACTTTCTTGCATGAAGAAGGGCCTTTCCAGGCGATGTACAATGAATTTTCAACCGCCAACATACAGATTGATAAAGATTTTAGTGCAAAGCTTTCAGGATATGGTTGTGTCGGCCAAATTCCCGAGGCAGAGATCTCCAATAGTTCCGTTGCAGTGGCAAATCTATCAGTGGAGACACTCGAGAGAGGAATGCTTACTCCAAAAAGCAATGTCTGGAGTTTCGGAATTGTTCTTCTGGAACTACTCACTGGCCGGAGGAATCTAGACAGCCGTCACCCCAAGGAAGAGAGGAATTTAGTTAAGTGGAGCCGGCCTTTCTTAGCCGATGACTGCCGACTTTCACTAATCATGGATCCTCAGCTGAAAGGTCGCTTCCCATCCCAAGCAGCCAGAAGGGTGTCAGACATTGCACAGAAATGCCTTCAGAAGGACCCATCAGAAAGGCCTACCATGAGAACCGTAGTTCAGCATCTCAAGATCGATAGAAGACATGAAGCACTCGTGCAGGTTTCCATTGCAAGAGCCAGCATTTGCCGGAAAATAAATGTCAAGATCACCCAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

482

Amino Acids

53.52

Weight (kDa)

9.32

Isoelectric Point (pI)

49.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 57
Acc36I ACCTGC 1 cut(s) 1375
AciI CCGC 2 cut(s) 357, 933
AclWI GGATC 2 cut(s) 1226, 1239
AcoI YGGCCR 2 cut(s) 991, 1129
AcsI RAATTY 3 cut(s) 923, 996, 1171
AcuI CTGAAG 2 cut(s) 516, 1286
AcvI CACGTG 1 cut(s) 737
AfaI GTAC 2 cut(s) 284, 917
AfiI CCNNNNNNNGG 2 cut(s) 442, 1306
AgeI ACCGGT 1 cut(s) 316
AgsI TTSAA 5 cut(s) 22, 170, 606, 617, 930
AjnI CCWGG 3 cut(s) 289, 348, 906
AloI GAACNNNNNNTCC 2 cut(s) 93, 125
AluBI AGCT 6 cut(s) 89, 215, 515, 620, 970, 1240
AluI AGCT 6 cut(s) 89, 215, 515, 620, 970, 1240
Alw21I GWGCWC 1 cut(s) 202
Alw26I GTCTC 1 cut(s) 1046
AlwI GGATC 2 cut(s) 1226, 1239
Ama87I CYCGRG 3 cut(s) 139, 1001, 1058
AoxI GGCC 8 cut(s) 287, 403, 553, 899, 991, 1129, 1190, 1322
ApeKI GCWGC 6 cut(s) 89, 233, 359, 446, 866, 1265
ApoI RAATTY 3 cut(s) 923, 996, 1171
AsiGI ACCGGT 1 cut(s) 316
AspLEI GCGC 1 cut(s) 866
AspS9I GGNCC 4 cut(s) 404, 422, 899, 1309
AsuHPI GGTGA 4 cut(s) 306, 629, 1145, 1426
AvaI CYCGRG 3 cut(s) 139, 1001, 1058
AvaII GGWCC 2 cut(s) 422, 1309
BamHI GGATCC 1 cut(s) 1231
BauI CACGAG 2 cut(s) 201, 1379
BbrPI CACGTG 1 cut(s) 737
BbsI GAAGAC 2 cut(s) 279, 1372
Bbv12I GWGCWC 1 cut(s) 202
BbvCI CCTCAGC 1 cut(s) 1236
BbvI GCAGC 6 cut(s) 76, 245, 346, 458, 853, 1277
BccI CCATC 5 cut(s) 226, 349, 649, 1264, 1321
BceAI ACGGC 2 cut(s) 459, 1134
BciT130I CCWGG 3 cut(s) 291, 350, 908
BcoDI GTCTC 1 cut(s) 1046
BfaI CTAG 1 cut(s) 1142
BfmI CTRYAG 1 cut(s) 125
BfuAI ACCTGC 1 cut(s) 1375
BglII AGATCT 1 cut(s) 1011
BisI GCNGC 7 cut(s) 90, 234, 357, 360, 447, 867, 1266
BlsI GCNGC 7 cut(s) 91, 235, 358, 361, 448, 868, 1267
Bme1390I CCNGG 3 cut(s) 291, 350, 908
Bme18I GGWCC 2 cut(s) 422, 1309
BmeT110I CYCGRG 3 cut(s) 139, 1001, 1058
BmgT120I GGNCC 4 cut(s) 404, 422, 899, 1309
BmiI GGNNCC 4 cut(s) 321, 1187, 1233, 1311
BmrFI CCNGG 3 cut(s) 291, 350, 908
BmsI GCATC 4 cut(s) 220, 518, 530, 1357
BoxI GACNNNNGTC 1 cut(s) 1149
BpiI GAAGAC 2 cut(s) 279, 1372
BpmI CTGGAG 1 cut(s) 1114
Bpu10I CCTNAGC 1 cut(s) 1236
BpuEI CTTGAG 1 cut(s) 1337
Bsa29I ATCGAT 1 cut(s) 1359
BsaAI YACGTR 1 cut(s) 737
BsaJI CCNNGG 3 cut(s) 348, 1002, 1158
BsaWI WCCGGW 1 cut(s) 316
Bsc4I CCNNNNNNNGG 2 cut(s) 442, 1306
Bse118I RCCGGY 4 cut(s) 285, 316, 795, 1188
Bse1I ACTGG 5 cut(s) 173, 463, 478, 839, 1132
Bse3DI GCAATG 5 cut(s) 92, 425, 1093, 1284, 1392
BseBI CCWGG 3 cut(s) 291, 350, 908
BseCI ATCGAT 1 cut(s) 1359
BseDI CCNNGG 3 cut(s) 348, 1002, 1158
BseGI GGATG 3 cut(s) 641, 700, 1256
BseLI CCNNNNNNNGG 2 cut(s) 442, 1306
BseMI GCAATG 5 cut(s) 92, 425, 1093, 1284, 1392
BseMII CTCAG 4 cut(s) 33, 356, 662, 1250
BseNI ACTGG 5 cut(s) 173, 463, 478, 839, 1132
BseXI GCAGC 6 cut(s) 76, 245, 346, 458, 853, 1277
BseYI CCCAGC 1 cut(s) 1437
BsgI GTGCAG 4 cut(s) 117, 345, 852, 1403
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 8 cut(s) 289, 405, 555, 901, 993, 1131, 1192, 1324
BshTI ACCGGT 1 cut(s) 316
BshVI ATCGAT 1 cut(s) 1359
BsiEI CGRYCG 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 202
BsiHKCI CYCGRG 3 cut(s) 139, 1001, 1058
BsiSI CCGG 6 cut(s) 286, 317, 796, 1132, 1189, 1414
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 2 cut(s) 442, 1306
BsmAI GTCTC 1 cut(s) 1046
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 1074
BsnI GGCC 8 cut(s) 289, 405, 555, 901, 993, 1131, 1192, 1324
BsoBI CYCGRG 3 cut(s) 139, 1001, 1058
Bsp1286I GDGCHC 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 915
Bsp143I GATC 7 cut(s) 119, 247, 812, 1011, 1231, 1356, 1431
BspACI CCGC 2 cut(s) 357, 933
BspANI GGCC 8 cut(s) 289, 405, 555, 901, 993, 1131, 1192, 1324
BspCNI CTCAG 4 cut(s) 34, 357, 661, 1249
BspDI ATCGAT 1 cut(s) 1359
BspLI GGNNCC 4 cut(s) 321, 1187, 1233, 1311
BspMI ACCTGC 1 cut(s) 1375
BspPI GGATC 2 cut(s) 1226, 1239
BsrDI GCAATG 5 cut(s) 92, 425, 1093, 1284, 1392
BsrFI RCCGGY 4 cut(s) 285, 316, 795, 1188
BsrGI TGTACA 1 cut(s) 915
BsrI ACTGG 5 cut(s) 173, 463, 478, 839, 1132
BssAI RCCGGY 4 cut(s) 285, 316, 795, 1188
BssECI CCNNGG 3 cut(s) 348, 1002, 1158
BssMI GATC 7 cut(s) 119, 247, 812, 1011, 1231, 1356, 1431
BssSI CACGAG 2 cut(s) 201, 1379
BssT1I CCWWGG 1 cut(s) 1158
Bst2BI CACGAG 2 cut(s) 201, 1379
Bst2UI CCWGG 3 cut(s) 291, 350, 908
Bst4CI ACNGT 3 cut(s) 129, 625, 1339
Bst6I CTCTTC 2 cut(s) 336, 1158
BstAUI TGTACA 1 cut(s) 915
BstBAI YACGTR 1 cut(s) 737
BstC8I GCNNGC 3 cut(s) 517, 1190, 1405
BstDEI CTNAG 5 cut(s) 42, 365, 648, 1198, 1236
BstENI CCTNNNNNAGG 1 cut(s) 1304
BstF5I GGATG 3 cut(s) 641, 700, 1256
BstHHI GCGC 1 cut(s) 866
BstKTI GATC 7 cut(s) 122, 250, 815, 1014, 1234, 1359, 1434
BstMAI GTCTC 1 cut(s) 1046
BstMBI GATC 7 cut(s) 119, 247, 812, 1011, 1231, 1356, 1431
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 2 cut(s) 212, 863
BstNI CCWGG 3 cut(s) 291, 350, 908
BstNSI RCATGY 1 cut(s) 547
BstPAI GACNNNNGTC 1 cut(s) 1149
BstSCI CCNGG 3 cut(s) 289, 348, 906
BstSFI CTRYAG 1 cut(s) 125
BstV1I GCAGC 6 cut(s) 76, 245, 346, 458, 853, 1277
BstV2I GAAGAC 2 cut(s) 279, 1372
BstX2I RGATCY 2 cut(s) 1011, 1231
BstYI RGATCY 2 cut(s) 1011, 1231
Bsu15I ATCGAT 1 cut(s) 1359
BsuRI GGCC 8 cut(s) 289, 405, 555, 901, 993, 1131, 1192, 1324
BsuTUI ATCGAT 1 cut(s) 1359
BtgZI GCGATG 2 cut(s) 327, 926
BtsCI GGATG 3 cut(s) 641, 700, 1256
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 5 cut(s) 362, 396, 1038, 1053, 1125
BveI ACCTGC 1 cut(s) 1375
Cac8I GCNNGC 3 cut(s) 517, 1190, 1405
CfoI GCGC 1 cut(s) 866
Cfr10I RCCGGY 4 cut(s) 285, 316, 795, 1188
Cfr13I GGNCC 4 cut(s) 404, 422, 899, 1309
ClaI ATCGAT 1 cut(s) 1359
Csp6I GTAC 2 cut(s) 283, 916
CspAI ACCGGT 1 cut(s) 316
CviAII CATG 7 cut(s) 544, 731, 782, 890, 1228, 1330, 1370
CviQI GTAC 2 cut(s) 283, 916
DdeI CTNAG 5 cut(s) 42, 365, 648, 1198, 1236
DpnI GATC 7 cut(s) 121, 249, 814, 1013, 1233, 1358, 1433
DpnII GATC 7 cut(s) 119, 247, 812, 1011, 1231, 1356, 1431
EaeI YGGCCR 2 cut(s) 991, 1129
Eam1104I CTCTTC 2 cut(s) 336, 1158
EarI CTCTTC 2 cut(s) 336, 1158
Eco130I CCWWGG 1 cut(s) 1158
Eco147I AGGCCT 2 cut(s) 555, 1324
Eco47I GGWCC 2 cut(s) 422, 1309
Eco57I CTGAAG 2 cut(s) 516, 1286
Eco72I CACGTG 1 cut(s) 737
Eco88I CYCGRG 3 cut(s) 139, 1001, 1058
EcoNI CCTNNNNNAGG 1 cut(s) 1304
EcoO109I RGGNCCY 2 cut(s) 899, 1309
EcoRII CCWGG 3 cut(s) 289, 348, 906
EcoT14I CCWWGG 1 cut(s) 1158
EcoT22I ATGCAT 1 cut(s) 545
ErhI CCWWGG 1 cut(s) 1158
FaeI CATG 7 cut(s) 547, 734, 785, 893, 1231, 1333, 1373
FalI AAGNNNNNCTT 2 cut(s) 566, 598
FaqI GGGAC 1 cut(s) 53
FatI CATG 7 cut(s) 543, 730, 781, 889, 1227, 1329, 1369
Fnu4HI GCNGC 7 cut(s) 90, 234, 357, 360, 447, 867, 1266
FokI GGATG 3 cut(s) 628, 687, 1243
Fsp4HI GCNGC 7 cut(s) 90, 234, 357, 360, 447, 867, 1266
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 1 cut(s) 865
GluI GCNGC 7 cut(s) 90, 234, 357, 360, 447, 867, 1266
GsaI CCCAGC 1 cut(s) 1441
GsuI CTGGAG 1 cut(s) 1114
HaeIII GGCC 8 cut(s) 289, 405, 555, 901, 993, 1131, 1192, 1324
HapII CCGG 6 cut(s) 286, 317, 796, 1132, 1189, 1414
HhaI GCGC 1 cut(s) 866
Hin1II CATG 7 cut(s) 547, 734, 785, 893, 1231, 1333, 1373
Hin6I GCGC 1 cut(s) 864
HinP1I GCGC 1 cut(s) 864
HindIII AAGCTT 1 cut(s) 968
HinfI GANTC 4 cut(s) 183, 275, 593, 1138
HpaII CCGG 6 cut(s) 286, 317, 796, 1132, 1189, 1414
HphI GGTGA 4 cut(s) 306, 629, 1145, 1426
HpyAV CCTTC 7 cut(s) 67, 545, 644, 890, 1266, 1300, 1310
HpyCH4III ACNGT 3 cut(s) 129, 625, 1339
HpyCH4IV ACGT 1 cut(s) 736
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 863
HpyF3I CTNAG 5 cut(s) 42, 365, 648, 1198, 1236
HpySE526I ACGT 1 cut(s) 736
Hsp92II CATG 7 cut(s) 547, 734, 785, 893, 1231, 1333, 1373
HspAI GCGC 1 cut(s) 864
KroI GCCGGC 1 cut(s) 1188
KroNI GCCGGC 1 cut(s) 1190
Kzo9I GATC 7 cut(s) 119, 247, 812, 1011, 1231, 1356, 1431
LmnI GCTCC 3 cut(s) 76, 220, 1185
Lsp1109I GCAGC 6 cut(s) 76, 245, 346, 458, 853, 1277
LweI GCATC 4 cut(s) 220, 518, 530, 1357
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 1 cut(s) 736
MaeIII GTNAC 4 cut(s) 312, 391, 625, 1151
MalI GATC 7 cut(s) 121, 249, 814, 1013, 1233, 1358, 1433
MboI GATC 7 cut(s) 119, 247, 812, 1011, 1231, 1356, 1431
MfeI CAATTG 1 cut(s) 308
MflI RGATCY 2 cut(s) 1011, 1231
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 7 cut(s) 308, 716, 852, 923, 996, 1104, 1171
MlyI GAGTC 2 cut(s) 192, 269
MmeI TCCRAC 1 cut(s) 528
Mph1103I ATGCAT 1 cut(s) 545
MroNI GCCGGC 1 cut(s) 1188
MseI TTAA 5 cut(s) 177, 384, 656, 1179, 1444
MslI CAYNNNNRTG 2 cut(s) 735, 1294
MspA1I CMGCKG 3 cut(s) 89, 359, 1240
MspI CCGG 6 cut(s) 286, 317, 796, 1132, 1189, 1414
MspR9I CCNGG 3 cut(s) 291, 350, 908
MunI CAATTG 1 cut(s) 308
Mva1269I GAATGC 1 cut(s) 1074
MvaI CCWGG 3 cut(s) 291, 350, 908
MwoI GCNNNNNNNGC 2 cut(s) 212, 863
NaeI GCCGGC 1 cut(s) 1190
NdeII GATC 7 cut(s) 119, 247, 812, 1011, 1231, 1356, 1431
NgoMIV GCCGGC 1 cut(s) 1188
NlaIII CATG 7 cut(s) 547, 734, 785, 893, 1231, 1333, 1373
NlaIV GGNNCC 4 cut(s) 321, 1187, 1233, 1311
NmuCI GTSAC 3 cut(s) 312, 391, 1151
NsiI ATGCAT 1 cut(s) 545
NspI RCATGY 1 cut(s) 547
PaeR7I CTCGAG 1 cut(s) 1058
PceI AGGCCT 2 cut(s) 555, 1324
PctI GAATGC 1 cut(s) 1074
PdiI GCCGGC 1 cut(s) 1190
PfeI GAWTC 2 cut(s) 593, 1138
PinAI ACCGGT 1 cut(s) 316
PkrI GCNGC 7 cut(s) 91, 235, 358, 361, 448, 868, 1267
Ple19I CGATCG 1 cut(s) 122
PleI GAGTC 2 cut(s) 191, 269
PmaCI CACGTG 1 cut(s) 737
PmlI CACGTG 1 cut(s) 737
PpsI GAGTC 2 cut(s) 191, 269
Ppu21I YACGTR 1 cut(s) 737
PpuMI RGGWCCY 1 cut(s) 1309
PshAI GACNNNNGTC 1 cut(s) 1149
PsiI TTATAA 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 1309
Psp6I CCWGG 3 cut(s) 289, 348, 906
PspCI CACGTG 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 1437
PspGI CCWGG 3 cut(s) 289, 348, 906
PspN4I GGNNCC 4 cut(s) 321, 1187, 1233, 1311
PspPI GGNCC 4 cut(s) 404, 422, 899, 1309
PspPPI RGGWCCY 1 cut(s) 1309
PsrI GAACNNNNNNTAC 2 cut(s) 454, 486
PsuI RGATCY 2 cut(s) 1011, 1231
PvuI CGATCG 1 cut(s) 122
PvuII CAGCTG 2 cut(s) 89, 1240
RsaI GTAC 2 cut(s) 284, 917
RsaNI GTAC 2 cut(s) 283, 916
RseI CAYNNNNRTG 2 cut(s) 735, 1294
SaqAI TTAA 5 cut(s) 177, 384, 656, 1179, 1444
SatI GCNGC 7 cut(s) 90, 234, 357, 360, 447, 867, 1266
Sau3AI GATC 7 cut(s) 119, 247, 812, 1011, 1231, 1356, 1431
Sau96I GGNCC 4 cut(s) 404, 422, 899, 1309
SchI GAGTC 2 cut(s) 192, 269
ScrFI CCNGG 3 cut(s) 291, 350, 908
SduI GDGCHC 1 cut(s) 202
SfaNI GCATC 4 cut(s) 220, 518, 530, 1357
SfcI CTRYAG 1 cut(s) 125
Sfr274I CTCGAG 1 cut(s) 1058
SinI GGWCC 2 cut(s) 422, 1309
SlaI CTCGAG 1 cut(s) 1058
SmiMI CAYNNNNRTG 2 cut(s) 735, 1294
SmlI CTYRAG 2 cut(s) 1058, 1352
SmoI CTYRAG 2 cut(s) 1058, 1352
Sse9I AATT 7 cut(s) 308, 716, 852, 923, 996, 1104, 1171
SseBI AGGCCT 2 cut(s) 555, 1324
SsiI CCGC 2 cut(s) 357, 933
SspMI CTAG 1 cut(s) 1142
StuI AGGCCT 2 cut(s) 555, 1324
StyD4I CCNGG 3 cut(s) 289, 348, 906
StyI CCWWGG 1 cut(s) 1158
TaaI ACNGT 3 cut(s) 129, 625, 1339
TaiI ACGT 1 cut(s) 739
TaqI TCGA 3 cut(s) 221, 1059, 1359
TaqII GACCGA 1 cut(s) 552
TasI AATT 7 cut(s) 308, 716, 852, 923, 996, 1104, 1171
TatI WGTACW 1 cut(s) 915
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 2 cut(s) 593, 1138
Tru1I TTAA 5 cut(s) 177, 384, 656, 1179, 1444
Tru9I TTAA 5 cut(s) 177, 384, 656, 1179, 1444
TscAI CASTG 5 cut(s) 369, 396, 1038, 1053, 1132
TseFI GTSAC 3 cut(s) 312, 391, 1151
TseI GCWGC 6 cut(s) 89, 233, 359, 446, 866, 1265
Tsp45I GTSAC 3 cut(s) 312, 391, 1151
TspDTI ATGAA 8 cut(s) 276, 513, 585, 655, 863, 906, 936, 1386
TspGWI ACGGA 2 cut(s) 499, 1015
TspRI CASTG 5 cut(s) 369, 396, 1038, 1053, 1132
VpaK11BI GGWCC 2 cut(s) 422, 1309
XagI CCTNNNNNAGG 1 cut(s) 1304
XapI RAATTY 3 cut(s) 923, 996, 1171
XbaI TCTAGA 1 cut(s) 1141
XceI RCATGY 1 cut(s) 547
XhoI CTCGAG 1 cut(s) 1058
XspI CTAG 1 cut(s) 1142
Zsp2I ATGCAT 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.