MD04G1189100.v1.1

NAD(P)H-quinone oxidoreductase chain 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0020222
Physical Location & Seq
Reverse (-)
27958431 .. 27959151
721 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1189100.v1.1.491

Sequence Viewer

Length: 654 bp
ATGAGGGCCTTTTTCTTTTTAACAATGATGTTATGTTTCAATATAATGTTAGGGTTTAGAGGGTTAGTTTTCTTTCTTATACAAGAAAAAATGAGTACTTGTATGCTTTTAGCCGGAATCTTATTAAAAATGGGACCGTATGGATTGGTTCAGATCAATATGGAATTATTACCCCACGCTCATTCTATATTCTCTCCCTGGTTGATTATAGTAGGCACAATTCAAATAATCTATGCAGCTTCAACATCTCCCGGAATTGGTTCTATAAGCGATACAAGACTCAACGGAGCCATTTTACAAATAATCTCTCACGGCTTTATTGGCGCTGCGCTTTTTTTCTTGGCGAGAACGAGTTATAATAGAATACATCTTGTTTATCTCAACGAAATGGGCGGAATGGCTATCGCAATTCTAAAAATATTCCCGACTTTCAGTATCTTATCAATGGCTTCTCTTGCATTACCGGGCATGAGCGGTTTTGTTGCCGAATTGTTAGTTTTTTTTGGAATACTTACTAGTCAAAAATATCTTTTAATGACAAAAATATTAATAACTTTTGTAATGGCAATTGGAATGATATTAACTCCTATTTATTCATTATCTATGTTACGCCATATGTTCTATGGATACAAGCTTTTTAAGTTCCTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Fer4 Proton_antipo_M

Protein Analysis

218

Amino Acids

24.26

Weight (kDa)

9.81

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 94 - 187 8.5e-09 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020222)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01050
malus_domestica MD04G1189100.v1.1
rosa_chinensis RchiOBHm_Chr5g0017231 RchiOBHm_Chr5g0043051 RchiOBHm_CPg0502791
rosa_samantha Rh5BG295100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 357
AccBSI CCGCTC 1 cut(s) 474
AciI CCGC 2 cut(s) 393, 474
AfaI GTAC 1 cut(s) 97
AfiI CCNNNNNNNGG 1 cut(s) 257
AgsI TTSAA 3 cut(s) 40, 224, 243
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 1 cut(s) 197
AluBI AGCT 2 cut(s) 239, 634
AluI AGCT 2 cut(s) 239, 634
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 236, 326
AseI ATTAAT 1 cut(s) 548
Asp700I GAANNNNTTC 1 cut(s) 259
AspLEI GCGC 2 cut(s) 326, 331
AspS9I GGNCC 2 cut(s) 6, 134
AsuC2I CCSGG 2 cut(s) 252, 465
AvaII GGWCC 1 cut(s) 134
BbvI GCAGC 2 cut(s) 248, 313
BceAI ACGGC 1 cut(s) 328
BciT130I CCWGG 1 cut(s) 199
BciVI GTATCC 1 cut(s) 620
BcnI CCSGG 2 cut(s) 252, 465
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BfoI RGCGCY 1 cut(s) 327
BfuI GTATCC 1 cut(s) 620
BisI GCNGC 2 cut(s) 237, 327
BlsI GCNGC 2 cut(s) 238, 328
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 3 cut(s) 199, 252, 465
Bme18I GGWCC 1 cut(s) 134
BmgT120I GGNCC 2 cut(s) 6, 134
BmiI GGNNCC 2 cut(s) 135, 289
BmrFI CCNGG 3 cut(s) 199, 252, 465
BpuMI CCSGG 2 cut(s) 252, 465
BsaJI CCNNGG 1 cut(s) 197
Bsc4I CCNNNNNNNGG 1 cut(s) 257
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 197
BseLI CCNNNNNNNGG 1 cut(s) 257
BseXI GCAGC 2 cut(s) 248, 313
BshFI GGCC 1 cut(s) 8
BsiSI CCGG 3 cut(s) 114, 252, 464
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 257
BsmFI GGGAC 1 cut(s) 147
BsnI GGCC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 153
BspACI CCGC 2 cut(s) 393, 474
BspANI GGCC 1 cut(s) 8
BspLI GGNNCC 2 cut(s) 135, 289
BsrBI CCGCTC 1 cut(s) 474
BssECI CCNNGG 1 cut(s) 197
BssMI GATC 1 cut(s) 153
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 2 cut(s) 326, 331
BstKTI GATC 1 cut(s) 156
BstMBI GATC 1 cut(s) 153
BstMWI GCNNNNNNNGC 2 cut(s) 321, 455
BstNI CCWGG 1 cut(s) 199
BstSCI CCNGG 3 cut(s) 197, 250, 463
BstV1I GCAGC 2 cut(s) 248, 313
BsuI GTATCC 1 cut(s) 620
BsuRI GGCC 1 cut(s) 8
CfoI GCGC 2 cut(s) 326, 331
Cfr13I GGNCC 2 cut(s) 6, 134
Csp6I GTAC 1 cut(s) 96
CviAII CATG 1 cut(s) 469
CviJI RGCY 8 cut(s) 8, 113, 239, 290, 315, 401, 449, 634
CviKI_1 RGCY 8 cut(s) 8, 113, 239, 290, 315, 401, 449, 634
CviQI GTAC 1 cut(s) 96
DpnI GATC 1 cut(s) 155
DpnII GATC 1 cut(s) 153
EciI GGCGGA 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 197
FaeI CATG 1 cut(s) 472
FaqI GGGAC 1 cut(s) 147
FatI CATG 1 cut(s) 468
FauNDI CATATG 1 cut(s) 615
Fnu4HI GCNGC 2 cut(s) 237, 327
Fsp4HI GCNGC 2 cut(s) 237, 327
FspBI CTAG 1 cut(s) 516
GlaI GCGC 2 cut(s) 325, 330
GluI GCNGC 2 cut(s) 237, 327
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 1 cut(s) 8
HapII CCGG 3 cut(s) 114, 252, 464
HhaI GCGC 2 cut(s) 326, 331
Hin1II CATG 1 cut(s) 472
Hin6I GCGC 2 cut(s) 324, 329
HinP1I GCGC 2 cut(s) 324, 329
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 2 cut(s) 117, 279
HpaII CCGG 3 cut(s) 114, 252, 464
Hpy188I TCNGA 1 cut(s) 153
Hpy188III TCNNGA 1 cut(s) 424
HpyCH4III ACNGT 1 cut(s) 138
HpyCH4V TGCA 2 cut(s) 236, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 321, 455
Hsp92II CATG 1 cut(s) 472
HspAI GCGC 2 cut(s) 324, 329
Kzo9I GATC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 287
LpnPI CCDG 5 cut(s) 127, 184, 211, 265, 477
Lsp1109I GCAGC 2 cut(s) 248, 313
MaeI CTAG 1 cut(s) 516
MaeIII GTNAC 1 cut(s) 606
MalI GATC 1 cut(s) 155
MbiI CCGCTC 1 cut(s) 474
MboI GATC 1 cut(s) 153
MfeI CAATTG 1 cut(s) 567
MluCI AATT 6 cut(s) 164, 219, 255, 408, 488, 567
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 1 cut(s) 53
MroXI GAANNNNTTC 1 cut(s) 259
MseI TTAA 6 cut(s) 20, 125, 533, 548, 581, 639
MspI CCGG 3 cut(s) 114, 252, 464
MspR9I CCNGG 3 cut(s) 199, 252, 465
MteI GCGCNGCGC 1 cut(s) 327
MunI CAATTG 1 cut(s) 567
MvaI CCWGG 1 cut(s) 199
MwoI GCNNNNNNNGC 2 cut(s) 321, 455
NciI CCSGG 2 cut(s) 252, 465
NdeI CATATG 1 cut(s) 615
NdeII GATC 1 cut(s) 153
NlaIII CATG 1 cut(s) 472
NlaIV GGNNCC 2 cut(s) 135, 289
PdmI GAANNNNTTC 1 cut(s) 259
PfeI GAWTC 1 cut(s) 117
PfoI TCCNGGA 1 cut(s) 250
PkrI GCNGC 2 cut(s) 238, 328
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
PshBI ATTAAT 1 cut(s) 548
PsiI TTATAA 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 197
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 135, 289
PspPI GGNCC 2 cut(s) 6, 134
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
SaqAI TTAA 6 cut(s) 20, 125, 533, 548, 581, 639
SatI GCNGC 2 cut(s) 237, 327
Sau3AI GATC 1 cut(s) 153
Sau96I GGNCC 2 cut(s) 6, 134
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 3 cut(s) 199, 252, 465
SetI ASST 2 cut(s) 241, 636
SinI GGWCC 1 cut(s) 134
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 6 cut(s) 164, 219, 255, 408, 488, 567
SsiI CCGC 2 cut(s) 393, 474
SspI AATATT 2 cut(s) 420, 546
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 3 cut(s) 197, 250, 463
TaaI ACNGT 1 cut(s) 138
TasI AATT 6 cut(s) 164, 219, 255, 408, 488, 567
TatI WGTACW 1 cut(s) 95
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 6 cut(s) 20, 125, 533, 548, 581, 639
Tru9I TTAA 6 cut(s) 20, 125, 533, 548, 581, 639
TseI GCWGC 2 cut(s) 236, 326
TspDTI ATGAA 1 cut(s) 585
TspGWI ACGGA 1 cut(s) 300
VpaK11BI GGWCC 1 cut(s) 134
VspI ATTAAT 1 cut(s) 548
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmnI GAANNNNTTC 1 cut(s) 259
XspI CTAG 1 cut(s) 516
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.