RchiOBHm_Chr5g0043051

NAD(P)H-quinone oxidoreductase chain 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020222
Physical Location & Seq
Forward (+)
37828619 .. 37830121
1503 bp
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UTR
Exon/CDS
Intron
PRQ32138

Sequence Viewer

Length: 1503 bp
ATGAATAATTTTCCTTGGTTAACGCTAGTTGTAGTTTTGCCAATAACCGCAGGTTCCTTAATTTTCTTTCTTCCTCATAGAGGAAATAAGGTAATAAGATGGTATACTATATCTATATGCATAACGGAACTCCTTCTAACAACCTATGCATTCGGTTATCATTTCCAATTGGATGATCCGTTAATGCAACTAACGGAGAATTATCAATGGATCAATTTTTTTGATTTTTACTGGAGATTGGGAATAGATGGAATTTCTATAGGACCCATTTTACTGACAGGATTTATCACAACTTTAGCTACTTTAGCGGCTTGGCCCGTTACTCGAGATTCCCTACTATTCCATTTCCTAATGTTAGCAATGTATAGCGGTCAAATAGGACCATTTTCTTCTCAAGACCTTTTACTTTTTTTCATCATGTGGGAGTTAGAATTAATTCCCGTTTATCTACTTCTATCCATGTGGGGGGGAAAGAAACGTTTGTATTCAGCTACAAAATTTATTTTGTACACTGCCGGAGGTTCCGTTTTTTTATTAATAGGAGTTCTGGGTATTGGTTTATATGGCTCCACTGAACCGACATTAAATTTTGAAACATCAGCTAATCAATCGTATCCTGTAGCCCTGGAAATAATATTCTATATTGGATTTCTTATTGCTTTTGCTGTCAAATCACCGATCATACCCCTACACACATGGTTACCAGACACCCATGGAGAGGCACATTATAGTACTTGTATGCTTTTAGCCGGAATCTTATTAAAAATGGGAGCGTATGGGTTGGTTCGGATCAATATGGAATTATTACCCCATGCCCATTCTATATTTTCTCCCTGGTTGATGATAGTGGGCACAATTCAAATTATTTATGCAGGTTTAACATCTCCTGGTCAGCGTAATTTAAAAAAAAGAATAGCCTATTCCTCTGTATCTCATATGGGTTTCATAATTATAGGAATTGGTTCTATAAGCGATACAGGGCTCAATGGGGCCATTTTACAAATAATTTCTCACGGATTTATTGGCGCTGCGCTTTTTTTCTTGGCCGGAACGAGTTATGATAGAATACGACTTATTTATCTCGACGAAATGGGCGGAATGGCTATCGCAATTCCAAAAATATTCACGACTTTCAGTATCTTATCAATGGCTTCGCTTGCATTACCGGGCATGAGCGGTTTTGTTGCCGAATTGATAATTTTTTTTGGAATACTTACTAGCCAAAAATATCTTTTAATGACAAAAGTATTAATTACTTTTGTAATGGCAATTGGAATGATATTAACTCCTATTTATTCATTATCTATGTTACGCCAGATGTTCTATGGATACAAGCTTTTTAATGCCCCAAACTCTTATTTTTTTGATTCTGGACCGCGAGAGTTAGTTATTTCTATTTCTATCCTTTTACCTGTAATAGGTATTGGTATTTATCCCGATTTCGTTTTCTCATTATCAGTTGACAAGGTCGAAGCTATTATATCAAATTTTTTTTATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

500

Amino Acids

55.86

Weight (kDa)

7.15

Isoelectric Point (pI)

29.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 131 - 421 1.3e-59 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020222)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01050
malus_domestica MD04G1189100.v1.1
rosa_chinensis RchiOBHm_Chr5g0017231 RchiOBHm_Chr5g0043051 RchiOBHm_CPg0502791
rosa_samantha Rh5BG295100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 41, 863
AccBSI CCGCTC 1 cut(s) 1176
AccI GTMKAC 1 cut(s) 104
AccII CGCG 1 cut(s) 1378
AciI CCGC 6 cut(s) 48, 308, 369, 1095, 1176, 1376
AclI AACGTT 1 cut(s) 478
AclWI GGATC 3 cut(s) 170, 218, 797
AcoI YGGCCR 1 cut(s) 1044
AcsI RAATTY 4 cut(s) 252, 497, 586, 1486
AfaI GTAC 2 cut(s) 509, 733
AfiI CCNNNNNNNGG 4 cut(s) 80, 465, 718, 1418
AgsI TTSAA 2 cut(s) 593, 860
AjnI CCWGG 3 cut(s) 624, 833, 886
AluBI AGCT 5 cut(s) 299, 491, 602, 1336, 1475
AluI AGCT 5 cut(s) 299, 491, 602, 1336, 1475
AlwI GGATC 3 cut(s) 170, 218, 797
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 3 cut(s) 314, 990, 1044
ApeKI GCWGC 1 cut(s) 1028
ApoI RAATTY 4 cut(s) 252, 497, 586, 1486
AseI ATTAAT 3 cut(s) 434, 536, 1250
Asp700I GAANNNNTTC 3 cut(s) 132, 435, 961
AspLEI GCGC 2 cut(s) 1028, 1033
AspS9I GGNCC 5 cut(s) 263, 315, 380, 990, 1373
AsuC2I CCSGG 1 cut(s) 1167
AsuHPI GGTGA 1 cut(s) 666
AvaI CYCGRG 1 cut(s) 324
AvaII GGWCC 3 cut(s) 263, 380, 1373
BaeGI GKGCMC 1 cut(s) 854
BanII GRGCYC 1 cut(s) 984
BbvI GCAGC 1 cut(s) 1015
BccI CCATC 2 cut(s) 93, 242
BciT130I CCWGG 3 cut(s) 626, 835, 888
BciVI GTATCC 2 cut(s) 624, 1322
BcnI CCSGG 1 cut(s) 1167
BfaI CTAG 2 cut(s) 26, 1218
BfmI CTRYAG 2 cut(s) 258, 618
BfoI RGCGCY 1 cut(s) 1029
BfuAI ACCTGC 2 cut(s) 41, 863
BfuI GTATCC 2 cut(s) 624, 1322
BisI GCNGC 2 cut(s) 309, 1029
BlsI GCNGC 2 cut(s) 310, 1030
BmcAI AGTACT 1 cut(s) 733
Bme1390I CCNGG 4 cut(s) 626, 835, 888, 1167
Bme18I GGWCC 3 cut(s) 263, 380, 1373
BmeT110I CYCGRG 1 cut(s) 324
BmgT120I GGNCC 5 cut(s) 263, 315, 380, 990, 1373
BmiI GGNNCC 5 cut(s) 55, 265, 523, 568, 991
BmrFI CCNGG 4 cut(s) 626, 835, 888, 1167
BpmI CTGGAG 1 cut(s) 253
BpuEI CTTGAG 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 1167
BsaJI CCNNGG 4 cut(s) 14, 624, 712, 833
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 465, 718, 1418
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 366
BseBI CCWGG 3 cut(s) 626, 835, 888
BseDI CCNNGG 4 cut(s) 14, 624, 712, 833
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 4 cut(s) 80, 465, 718, 1418
BseMI GCAATG 1 cut(s) 366
BseNI ACTGG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 854
BseXI GCAGC 1 cut(s) 1015
Bsh1236I CGCG 1 cut(s) 1378
BshFI GGCC 3 cut(s) 316, 992, 1046
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 4 cut(s) 516, 750, 1047, 1166
BslI CCNNNNNNNGG 4 cut(s) 80, 465, 718, 1418
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 316, 992, 1046
BsoBI CYCGRG 1 cut(s) 324
Bsp1286I GDGCHC 2 cut(s) 854, 984
Bsp1407I TGTACA 1 cut(s) 507
Bsp143I GATC 4 cut(s) 175, 210, 678, 789
Bsp19I CCATGG 1 cut(s) 712
BspACI CCGC 6 cut(s) 48, 308, 369, 1095, 1176, 1376
BspANI GGCC 3 cut(s) 316, 992, 1046
BspFNI CGCG 1 cut(s) 1378
BspLI GGNNCC 5 cut(s) 55, 265, 523, 568, 991
BspMI ACCTGC 2 cut(s) 41, 863
BspPI GGATC 3 cut(s) 170, 218, 797
BsrBI CCGCTC 1 cut(s) 1176
BsrDI GCAATG 1 cut(s) 366
BsrGI TGTACA 1 cut(s) 507
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 4 cut(s) 14, 624, 712, 833
BssMI GATC 4 cut(s) 175, 210, 678, 789
BssNAI GTATAC 1 cut(s) 105
BssT1I CCWWGG 2 cut(s) 14, 712
Bst1107I GTATAC 1 cut(s) 105
Bst2UI CCWGG 3 cut(s) 626, 835, 888
BstAUI TGTACA 1 cut(s) 507
BstC8I GCNNGC 1 cut(s) 1158
BstDSI CCRYGG 1 cut(s) 712
BstEII GGTNACC 1 cut(s) 699
BstENI CCTNNNNNAGG 2 cut(s) 78, 1416
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 1 cut(s) 1378
BstH2I RGCGCY 1 cut(s) 1029
BstHHI GCGC 2 cut(s) 1028, 1033
BstKTI GATC 4 cut(s) 178, 213, 681, 792
BstMBI GATC 4 cut(s) 175, 210, 678, 789
BstMWI GCNNNNNNNGC 2 cut(s) 305, 1157
BstNI CCWGG 3 cut(s) 626, 835, 888
BstPI GGTNACC 1 cut(s) 699
BstSCI CCNGG 4 cut(s) 624, 833, 886, 1165
BstSFI CTRYAG 2 cut(s) 258, 618
BstSLI GKGCMC 1 cut(s) 854
BstUI CGCG 1 cut(s) 1378
BstV1I GCAGC 1 cut(s) 1015
BstZ17I GTATAC 1 cut(s) 105
BsuI GTATCC 2 cut(s) 624, 1322
BsuRI GGCC 3 cut(s) 316, 992, 1046
BtgI CCRYGG 1 cut(s) 712
BtsCI GGATG 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 510
BtsIMutI CAGTG 2 cut(s) 510, 570
BveI ACCTGC 2 cut(s) 41, 863
Cac8I GCNNGC 1 cut(s) 1158
CfoI GCGC 2 cut(s) 1028, 1033
Cfr13I GGNCC 5 cut(s) 263, 315, 380, 990, 1373
Csp6I GTAC 2 cut(s) 508, 732
CviAII CATG 6 cut(s) 418, 460, 696, 713, 812, 1171
CviQI GTAC 2 cut(s) 508, 732
DpnI GATC 4 cut(s) 177, 212, 680, 791
DpnII GATC 4 cut(s) 175, 210, 678, 789
DraI TTTAAA 1 cut(s) 903
EaeI YGGCCR 1 cut(s) 1044
EciI GGCGGA 1 cut(s) 1110
Eco130I CCWWGG 2 cut(s) 14, 712
Eco24I GRGCYC 1 cut(s) 984
Eco47I GGWCC 3 cut(s) 263, 380, 1373
Eco88I CYCGRG 1 cut(s) 324
Eco91I GGTNACC 1 cut(s) 699
EcoNI CCTNNNNNAGG 2 cut(s) 78, 1416
EcoO109I RGGNCCY 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 699
EcoRII CCWGG 3 cut(s) 624, 833, 886
EcoT14I CCWWGG 2 cut(s) 14, 712
EcoT22I ATGCAT 2 cut(s) 122, 151
EcoT38I GRGCYC 1 cut(s) 984
ErhI CCWWGG 2 cut(s) 14, 712
FaeI CATG 6 cut(s) 421, 463, 699, 716, 815, 1174
FatI CATG 6 cut(s) 417, 459, 695, 712, 811, 1170
FauNDI CATATG 1 cut(s) 936
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 2 cut(s) 309, 1029
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 1 cut(s) 984
Fsp4HI GCNGC 2 cut(s) 309, 1029
FspBI CTAG 2 cut(s) 26, 1218
GlaI GCGC 2 cut(s) 1027, 1032
GluI GCNGC 2 cut(s) 309, 1029
GsuI CTGGAG 1 cut(s) 253
HaeII RGCGCY 1 cut(s) 1029
HaeIII GGCC 3 cut(s) 316, 992, 1046
HapII CCGG 4 cut(s) 516, 750, 1047, 1166
HhaI GCGC 2 cut(s) 1028, 1033
Hin1II CATG 6 cut(s) 421, 463, 699, 716, 815, 1174
Hin6I GCGC 2 cut(s) 1026, 1031
HinP1I GCGC 2 cut(s) 1026, 1031
HincII GTYRAC 2 cut(s) 21, 1462
HindII GTYRAC 2 cut(s) 21, 1462
HindIII AAGCTT 1 cut(s) 1334
HinfI GANTC 3 cut(s) 329, 753, 1367
HpaI GTTAAC 1 cut(s) 21
HpaII CCGG 4 cut(s) 516, 750, 1047, 1166
HphI GGTGA 1 cut(s) 666
Hpy166II GTNNAC 4 cut(s) 21, 105, 510, 1462
Hpy188I TCNGA 1 cut(s) 789
Hpy188III TCNNGA 6 cut(s) 326, 395, 1082, 1126, 1371, 1436
Hpy8I GTNNAC 4 cut(s) 21, 105, 510, 1462
Hpy99I CGWCG 1 cut(s) 1088
HpyAV CCTTC 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 5 cut(s) 120, 149, 187, 872, 1160
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 1157
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 6 cut(s) 421, 463, 699, 716, 815, 1174
HspAI GCGC 2 cut(s) 1026, 1031
KspAI GTTAAC 1 cut(s) 21
Kzo9I GATC 4 cut(s) 175, 210, 678, 789
LmnI GCTCC 2 cut(s) 572, 770
Lsp1109I GCAGC 1 cut(s) 1015
MaeI CTAG 2 cut(s) 26, 1218
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 3 cut(s) 319, 699, 1308
MalI GATC 4 cut(s) 177, 212, 680, 791
MbiI CCGCTC 1 cut(s) 1176
MboI GATC 4 cut(s) 175, 210, 678, 789
MboII GAAGA 2 cut(s) 62, 381
MfeI CAATTG 2 cut(s) 167, 1269
MhlI GDGCHC 2 cut(s) 854, 984
MnlI CCTC 5 cut(s) 74, 84, 512, 712, 934
Mph1103I ATGCAT 2 cut(s) 122, 151
MroXI GAANNNNTTC 3 cut(s) 132, 435, 961
MspI CCGG 4 cut(s) 516, 750, 1047, 1166
MspR9I CCNGG 4 cut(s) 626, 835, 888, 1167
MteI GCGCNGCGC 1 cut(s) 1029
MunI CAATTG 2 cut(s) 167, 1269
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 3 cut(s) 626, 835, 888
MvnI CGCG 1 cut(s) 1378
MwoI GCNNNNNNNGC 2 cut(s) 305, 1157
NciI CCSGG 1 cut(s) 1167
NcoI CCATGG 1 cut(s) 712
NdeI CATATG 1 cut(s) 936
NdeII GATC 4 cut(s) 175, 210, 678, 789
NlaIII CATG 6 cut(s) 421, 463, 699, 716, 815, 1174
NlaIV GGNNCC 5 cut(s) 55, 265, 523, 568, 991
NsiI ATGCAT 2 cut(s) 122, 151
PaeR7I CTCGAG 1 cut(s) 324
PctI GAATGC 1 cut(s) 149
PdmI GAANNNNTTC 3 cut(s) 132, 435, 961
PfeI GAWTC 3 cut(s) 329, 753, 1367
PflFI GACNNNGTC 1 cut(s) 1466
PkrI GCNGC 2 cut(s) 310, 1030
PpuMI RGGWCCY 1 cut(s) 263
PshBI ATTAAT 3 cut(s) 434, 536, 1250
Psp1406I AACGTT 1 cut(s) 478
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 3 cut(s) 624, 833, 886
PspEI GGTNACC 1 cut(s) 699
PspGI CCWGG 3 cut(s) 624, 833, 886
PspN4I GGNNCC 5 cut(s) 55, 265, 523, 568, 991
PspPI GGNCC 5 cut(s) 263, 315, 380, 990, 1373
PspPPI RGGWCCY 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 1466
RsaI GTAC 2 cut(s) 509, 733
RsaNI GTAC 2 cut(s) 508, 732
SatI GCNGC 2 cut(s) 309, 1029
Sau3AI GATC 4 cut(s) 175, 210, 678, 789
Sau96I GGNCC 5 cut(s) 263, 315, 380, 990, 1373
ScaI AGTACT 1 cut(s) 733
ScrFI CCNGG 4 cut(s) 626, 835, 888, 1167
SduI GDGCHC 2 cut(s) 854, 984
SfcI CTRYAG 2 cut(s) 258, 618
Sfr274I CTCGAG 1 cut(s) 324
SinI GGWCC 3 cut(s) 263, 380, 1373
SlaI CTCGAG 1 cut(s) 324
SmlI CTYRAG 2 cut(s) 324, 393
SmoI CTYRAG 2 cut(s) 324, 393
SsiI CCGC 6 cut(s) 48, 308, 369, 1095, 1176, 1376
SspI AATATT 2 cut(s) 636, 1122
SspMI CTAG 2 cut(s) 26, 1218
StyD4I CCNGG 4 cut(s) 624, 833, 886, 1165
StyI CCWWGG 2 cut(s) 14, 712
TaiI ACGT 1 cut(s) 481
TaqI TCGA 3 cut(s) 325, 1083, 1470
TatI WGTACW 2 cut(s) 507, 731
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 3 cut(s) 329, 753, 1367
TscAI CASTG 2 cut(s) 517, 577
TseI GCWGC 1 cut(s) 1028
TspDTI ATGAA 4 cut(s) 17, 403, 934, 1287
TspGWI ACGGA 5 cut(s) 140, 168, 209, 514, 1029
TspRI CASTG 2 cut(s) 517, 577
Tth111I GACNNNGTC 1 cut(s) 1466
VpaK11BI GGWCC 3 cut(s) 263, 380, 1373
VspI ATTAAT 3 cut(s) 434, 536, 1250
XagI CCTNNNNNAGG 2 cut(s) 78, 1416
XapI RAATTY 4 cut(s) 252, 497, 586, 1486
XcmI CCANNNNNNNNNTGG 1 cut(s) 1322
XhoI CTCGAG 1 cut(s) 324
XmiI GTMKAC 1 cut(s) 104
XmnI GAANNNNTTC 3 cut(s) 132, 435, 961
XspI CTAG 2 cut(s) 26, 1218
ZrmI AGTACT 1 cut(s) 733
Zsp2I ATGCAT 2 cut(s) 122, 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.